LOCUS       NZ_CP122696             4076 bp    DNA     circular CON 20-JUN-2024
DEFINITION  Escherichia coli strain ETEC4064 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP122696
VERSION     NZ_CP122696.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32549641
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Vereecke,N., Van Hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Virotyping and genetic antimicrobial susceptibility testing of
            porcine ETEC/STEC strains and associated plasmid types
  JOURNAL   Front Microbiol 14, 1139312 (2023)
   PUBMED   37143544
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Vereecke,N., Van hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2023) Department of Pathobiology, Pharmacology and
            Zoological Medicine, Universiteit Gent, Salisburylaan 133, Merelbeke
            9820, Belgium
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Trycycler; minimap2; medaka v. v0.5.3; v2.20;
            v1.5.0
            Genome Representation        :: Full
            Expected Final Version       :: Yes
            Genome Coverage              :: 323x
            Sequencing Technology        :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_030165955.1-RS_2024_06_20
            Annotation Date              :: 06/20/2024 04:20:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 3
            CDSs (total)                 :: 3
            Genes (coding)               :: 3
            CDSs (with protein)          :: 3
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP122696.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ETEC4064"
                     /isolation_source="feces"
                     /host="Sus scrofa"
                     /db_xref="taxon:562"
                     /plasmid="unnamed1"
                     /geo_loc_name="Belgium"
                     /collection_date="2021"
                     /collected_by="IMM_DI04"
     gene            1..2148
                     /gene="mobQ"
                     /locus_tag="QDW59_RS00005"
                     /old_locus_tag="QDW59_24700"
     CDS             1..2148
                     /gene="mobQ"
                     /locus_tag="QDW59_RS00005"
                     /old_locus_tag="QDW59_24700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_062873906.1"
                     /translation="MAIYHLSMKIISRSNGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWDEW
                     RREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(2406..3113)
                     /locus_tag="QDW59_RS00010"
                     /old_locus_tag="QDW59_24705"
     CDS             complement(2406..3113)
                     /locus_tag="QDW59_RS00010"
                     /old_locus_tag="QDW59_24705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296720.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001296720.1"
                     /translation="MTSNPLIAYKSNALVEASYKLTLQEQRFLLLCISRLNSGTDVASP
                     ELQKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRA
                     QYARGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYEILQQFRSTGE
                     RIIALDDFKSSLMLDGKYKDFKTLNRDLIKPCVDELNKKSDLAVTVETIKKGRTVVALH
                     FRFKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(3644..3898)
                     /gene="mobC"
                     /locus_tag="QDW59_RS00015"
                     /old_locus_tag="QDW59_24710"
     CDS             complement(3644..3898)
                     /gene="mobC"
                     /locus_tag="QDW59_RS00015"
                     /old_locus_tag="QDW59_24710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091749.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     Typing          2..359
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.214"
                     /coverage="99.0"
                     /evalue="1.43e-171"
     Typing          complement(3903..4076)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.286"
                     /coverage="98.0"
                     /evalue="2.6299999999999998e-81"
     Typing          complement(2407..3113)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..2145
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.636"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcgattt atcatctcag tatgaaaatc atctccagaa gcaacggcta cagcgccgtt
       61 gcttctgctg cctatcgttc tggttcacta atgcttgatg agcgtaccgg actgactcac
      121 gactacaccc gcaaaagcgg tgtggctgag gctgtgattc tgacccccgc gacagcccct
      181 gcctggtgta cgaaccgtgc cgaactctgg aatgccgttg agaaggccga acgccggaag
      241 aactcacaac ttgcgcggga aattgaactc gctatccccc gtgaactgcc gcaggatgcc
      301 gcacgggaaa ccgttctcgc ctttgtcaga gaaaactttg tcagtcaggg catgattgct
      361 gatgtggcgt tccatcacat ggataaaaca aatccccatg cgcacatcat gctgaccacg
      421 agggctgtcg gacctgctgg gttcggcgga aaggtcaggg actggaacga ccggacacat
      481 gccgagacgt ggcgtgcatc atgggctgac catgcaaaca gagcactggc gaacgccggt
      541 tatcaggaag agatagacca tcgttcatac gagcgacagg ggcttgagaa aacgcctggt
      601 attcacctcg gaaagtctgc ctgtgcgatg gaaacacgcg gtatcgaaac agaacgcggc
      661 gaacagaatc gtctgattaa caggcttaac ctggaaatac agatttcccg cactgaactc
      721 aggaacagcg ggctgacaga gcctgcccgc cgtgtggctg accttctgaa tatcgggctt
      781 cctgacaacg caacttcgta cacactacgt gacattatcg aagcgttgcc gaaggacagt
      841 aactcggcat ggaaactcac cagtaatgcc atgtcgatga tggcagatat gcaggcgacg
      901 cgcagacgct gggaagagct gaacaccgaa cgaaaggaag cgatgagcca tgctgccagc
      961 ctgaaaaaat catcgccgtt ttcgtccggt ttttcccgta ttccccttat gcaatgggcg
     1021 gcaccggaat accgtagtga gcagggaaaa atccagtctc tgagccagca aatggaaaaa
     1081 ttacgacagc attaccggaa ggtggagaaa caggacattc ccgcgagtca gatggcattt
     1141 gaaaaacagt ggcagcagtg gggggaatcc ggccttgcag ctctcagagc ccagttgctt
     1201 aaacgtgaag aggaattgcg ccggaaggaa caggaagata ctgaacgtag tcgcgcggag
     1261 cagctcagga aaaatgacaa tgccgttatg gattcagggg tgctgagagc agtcgttacg
     1321 gggtatggcg aggctcccat gcctggtaac ggggaaatga cctgttacct tctgcttcac
     1381 aaccggaacg gagaatatac gctctggggg aacgagctgg agaaataccg caccagaatc
     1441 tttgagagtg ttgatttaat gcgtgacagg agtggttata tctgtgaccg aagcgaaatc
     1501 ggccaacgtc cgccactgca acgtgtttat tcctctgcaa cctttgagca actgctgacg
     1561 caggtttgcc agacatggcc gcaacatacc cgcaatctcc gccagccgaa gacatggccg
     1621 gagtctttct gtcttggaga agaccgccag ccagccatgc catcactggc agcaaggaag
     1681 gtggatttta cgcagggacg tctgccaccg acgctgatgc cggtaatgag ttctgtcgac
     1741 cgggaaaccc gccagcttca gcttttgctg gtcatgggcg tggatgactc tctgggcggt
     1801 gttgtccggc ttaatggtac gctgtatccc gcgtttgccg ttccctcagc cgataacagc
     1861 cagctggtca tcagtgcact gaccgataag gggttacggt atgcagggta tggtgttgcc
     1921 gtaaatcacg atgcagacag ccacatatca cctgcgccgg aacttatgga gtttcatctg
     1981 aaaacccgtg aggcaccgct ttttgctgcc gtcaacacac cagaaaaaca gccggatcat
     2041 ctgttccgct cgctgggttt caatcgtacc tgggacgaat ggcggcgcga ggaagatgca
     2101 agaacacaca ccaccgagag gagacatgat cgcggttggt cacagtgaat gtgaacaata
     2161 ttttgttttg tttaacatcg tgttatgttc gtcgatgttc ttcgatgttc atcgacaggg
     2221 atgaatgcaa gaagcacact cacataatgc aagcatagtt ttgtttttcc gcccccaaaa
     2281 attgggggtt ctttttttgc tgcatcagag cgtttttgac gcaacttttc acttcaatgg
     2341 taccttcgta ttcctgacac aagaaaaacg cgacagtgag cgttttaggt gccttaaaat
     2401 cgattctaga tcgtcatctt gatctgtttg tcttccttga agcgaaaatg cagggctaca
     2461 accgtgcgcc cctttttgat cgtctcaacc gttacagcca ggtcgctttt tttgttcagt
     2521 tcgtcaacac agggttttat tagatccctg tttaacgttt tgaaatcttt atatttacca
     2581 tcaagcatca gtgatgattt aaaatcatcg agggcgataa tacgttcccc agtactgcgg
     2641 aattgctgga gaatttcgta tattcttatg ctgtaacttc ttgataaatt agaaatattt
     2701 ttaatgacga ctctggtgaa ttgccccttc aactgtgtca gataaggcat taccgcgtca
     2761 gaaaaagtga tttgtgcttt cccctctccc ctcgcgtact gtgcccgata ctgaatccag
     2821 cggaactcct cgcgcttctc gtcgtccttg agaatgatcg acctgtccca caaacgatcg
     2881 atcgcttcct gtagctgaac ctcggcattt ttccgcccca tatcagggaa tgaatcaaaa
     2941 tattccgcag ctgtaatggt cattgtcttt tgcaattcag gtgaagctac gtcagtaccg
     3001 gagttaagtc ggcttatgca gagcaataag aaacgctgtt cttgtaaagt tagcttgtaa
     3061 ctggcctcaa ccagcgcatt ggacttatag gctatcagag ggtttgatgt cattttaacc
     3121 accaggccaa aaatgaggtg aaataaaaac ctatcacctt tagcattggt gagcaattat
     3181 attttcaccc cccattttcc tgtgaatatt accttaaatc tcctcacctt taccttaaat
     3241 tacctcaccc aagcacctta aattatatca cctttacctt aaattccccc acctatgcac
     3301 gtgtaacgca ttgtttttaa tcaggaattt cgaccctaaa acaagtatta aaaacaagat
     3361 cttaaaaaca agatctaaaa acttaacggc gagcctgtgg ataacttggg gtgctgaaaa
     3421 taaaaatcaa ataaaaacaa tgtattgcaa aaaatcagga ttgaaaattc cgaatacacc
     3481 ccctccaacc ctgtggaaat ccagtattca tgcgggttca gcgcatatca cctgtcgaaa
     3541 aaccgtgcgt atgtgctcaa cctggcgact gattcacttc cgcttcctcg cccactcgct
     3601 cgctaacgtt cggtcgctgg gctgcggcga gcggacgata caatcactgc tctggcagcg
     3661 cctccagctc gttaatcagc ggcagtattc gcgtcacatc agcctgccgt gtcacctctg
     3721 ccttcaggaa ctggagtaac caagctcgca actgcacttc ctccggcttt ctgacctttg
     3781 ccagcaatgc tgcacccaca atgaccttct gcgctgtgtc caggctccgg ctcttcgcct
     3841 tcaggcgctg taatctggcc tcagcttcgg caatcttctg ttcgagtgtt ctgctcattt
     3901 cgtgactccg tgcgcggtga aaaatcgcat tttagcgcgt cactggtagt ttaaaaactg
     3961 agctggcata atgcacggca aatcacgaag tgcgcactta tacaatctcc actccgtttc
     4021 gattgtgtgc gccctgcggg gctgaaagaa aactgcaaaa aggcattacg gcagaa
//
