LOCUS       NZ_CP122632             6668 bp    DNA     circular CON 08-JUN-2024
DEFINITION  Escherichia coli strain ETEC4088 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP122632
VERSION     NZ_CP122632.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32549664
            Assembly: GCF_030013145.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6668)
  AUTHORS   Vereecke,N., Van Hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Virotyping and genetic antimicrobial susceptibility testing of
            porcine ETEC/STEC strains and associated plasmid types
  JOURNAL   Front Microbiol 14, 1139312 (2023)
   PUBMED   37143544
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6668)
  AUTHORS   Vereecke,N., Van hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2023) Department of Pathobiology, Pharmacology and
            Zoological Medicine, Universiteit Gent, Salisburylaan 133, Merelbeke
            9820, Belgium
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Trycycler; minimap2; medaka v. v0.5.3; v2.20;
            v1.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 229x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030013145.1-RS_2024_06_08
            Annotation Date                   :: 06/08/2024 04:59:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,120
            CDSs (total)                      :: 5,000
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 97 of 232
            Pseudo Genes (incomplete)         :: 149 of 232
            Pseudo Genes (internal stop)      :: 47 of 232
            Pseudo Genes (multiple problems)  :: 53 of 232
            Pseudo Genes (short protein)      :: 1 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP122632.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ETEC4088"
                     /isolation_source="feces"
                     /host="Sus scrofa"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="Belgium"
                     /collection_date="2012"
                     /collected_by="IMM_DI04"
     gene            1..1569
                     /gene="cea"
                     /locus_tag="QDW58_RS24795"
                     /old_locus_tag="QDW58_24795"
     CDS             1..1569
                     /gene="cea"
                     /locus_tag="QDW58_RS24795"
                     /old_locus_tag="QDW58_24795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            1955..2092
                     /locus_tag="QDW58_RS24800"
                     /old_locus_tag="QDW58_24800"
     CDS             1955..2092
                     /locus_tag="QDW58_RS24800"
                     /old_locus_tag="QDW58_24800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(2291..2449)
                     /locus_tag="QDW58_RS25645"
     CDS             complement(2291..2449)
                     /locus_tag="QDW58_RS25645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(3119..3310)
                     /locus_tag="QDW58_RS24805"
                     /old_locus_tag="QDW58_24805"
     CDS             complement(3119..3310)
                     /locus_tag="QDW58_RS24805"
                     /old_locus_tag="QDW58_24805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3374..3697
                     /locus_tag="QDW58_RS24810"
                     /old_locus_tag="QDW58_24810"
     CDS             3374..3697
                     /locus_tag="QDW58_RS24810"
                     /old_locus_tag="QDW58_24810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3906..>4337
                     /locus_tag="QDW58_RS24815"
                     /old_locus_tag="QDW58_24815"
                     /pseudo=""
     CDS             3906..>4337
                     /locus_tag="QDW58_RS24815"
                     /old_locus_tag="QDW58_24815"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4375..4893
                     /locus_tag="QDW58_RS24820"
                     /old_locus_tag="QDW58_24820"
     CDS             4375..4893
                     /locus_tag="QDW58_RS24820"
                     /old_locus_tag="QDW58_24820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            4900..5154
                     /locus_tag="QDW58_RS24825"
                     /old_locus_tag="QDW58_24825"
     CDS             4900..5154
                     /locus_tag="QDW58_RS24825"
                     /old_locus_tag="QDW58_24825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015059032.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5472..5891)
                     /locus_tag="QDW58_RS24830"
                     /old_locus_tag="QDW58_24830"
     CDS             complement(5472..5891)
                     /locus_tag="QDW58_RS24830"
                     /old_locus_tag="QDW58_24830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(5972..6409)
                     /locus_tag="QDW58_RS24835"
                     /old_locus_tag="QDW58_24835"
     CDS             complement(5972..6409)
                     /locus_tag="QDW58_RS24835"
                     /old_locus_tag="QDW58_24835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     Typing          2947..3034
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(2378..2933)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.699"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3688..5258
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.809"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggaaaccg cggtagcgta ctataaagat ggtgttcctt atgatgataa gggacaggta
       61 attattactc ttttgaatgg tactcctgac gggagtggct ctggcggcgg aggtggaaaa
      121 ggaggcagta aaagtgaaag ttctgcagct attcatgcaa ctgctaaatg gtctactgct
      181 caattaaaga aaacacaggc agagcaggct gcccgggcaa aagctgcagc ggaagcacag
      241 gcgaaagcaa aggcaaacag ggatgcgctg actcagcgcc tgaaggatat cgtgaatgag
      301 gctcttcgtc acaatgcctc acgtacgcct tcagcaacag agcttgctca tgctaataat
      361 gcagctatgc aggcggaagc agagcgtttg cgccttgcga aagcagaaga aaaagcccgt
      421 aaagaagcgg aagcagcaga aaaggctttt caggaagcag aacaacgacg taaagagatt
      481 gaacgggaga aggctgaaac agaacgccag ttgaaactgg ctgaagctga agagaaacga
      541 ctggctgcat tgagtgaaga agctaaagct gttgagatcg cccaaaaaaa actttctgct
      601 gcacaatctg aagtggtgaa aatggatgga gagattaaga ctctcaattc tcgtttaagc
      661 tccagtatcc atgcccgtga tgcagaaatg aaaacgctcg ctggaaaacg aaatgaactg
      721 gctcaggcat ccgctaaata taaagaactg gatgagctgg tcaaaaaact atcaccaaga
      781 gccaatgatc cgcttcagaa ccgtcctttt tttgaagcaa ccagacgacg ggttggggcc
      841 ggtaagatta gagaagaaaa acaaaaacag gtaacagcat cagaaacacg tattaaccgg
      901 ataaatgctg atataactca gatccagaag gctatttctc aggtcagtaa taatcgtaat
      961 gccggtatcg ctcgtgttca tgaagctgaa gaaaatttga aaaaagcaca gaataatctc
     1021 cttaattcac agattaagga tgctgttgat gcaacagtta gcttttatca aacgctgact
     1081 gaaaaatatg gtgaaaaata ttcgaaaatg gcacaggaac ttgctgataa gtctaaaggt
     1141 aagaaaatcg gcaatgtgaa tgaagctctc gctgcttttg aaaaatacaa ggatgtttta
     1201 aataagaaat tcagcaaagc cgatcgtgat gctattttta atgcgttggc atcggtgaag
     1261 tatgatgact gggctaaaca tttagatcag tttgccaagt acttgaagat tacggggcat
     1321 gtttcttttg gatatgatgt ggtatctgat atcctaaaaa ttaaggatac aggtgactgg
     1381 aagccactat ttcttacatt agagaagaaa gctgcagatg caggggtgag ttatgttgtt
     1441 gctttacttt ttagcttgct tgctggaact acattaggta tttggggtat tgctattgtt
     1501 acaggaattc tatgctccta tattgataag aataaactta atactataaa tgaggtgtta
     1561 gggatttaat tattctttat tgatataaaa agtcctagca atccaaatgg gattgctagg
     1621 accaaacaaa gtagattata tagcataaat aggtttaatt ttgctacggg ggcgttattt
     1681 aggttttttc ttctttcgaa aaaatctttc tttatgaagt taaaagctat gtattcaata
     1741 gcatattttg aatatggaca tagaatagtg cttatcacta ttgcatatag catcttatct
     1801 gacacaagga aataataccc ttcgctgttt tttgttataa ggtatatata tataagtgtg
     1861 cagtacaggc caaataaaat attttttatg tagtatctta agctcataaa ttaaacctcg
     1921 ccatatattc ttttcatttt ataaggatcg agttatgagg aaaagatttt ttgtgggaat
     1981 attcgcgata aacctccttg ttggatgtca ggctaactat atacgtgatg ttcagggagg
     2041 gaccatcgca ccatcctcct cttctaaact gacggggatc gcggttcagt agaaaagatc
     2101 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2161 ccaccgctac caacggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2221 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtcg
     2281 ggccactact tcaagaactc tgtagcaccg tttgtgccat catcgctctg ctaatccggt
     2341 taccagtggc tgctgccagt ggcgttaagg cgtgccttac cgggttggac tcaagacgat
     2401 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2461 tggagcgaac gacctacacc gaactgagat accaacagcg tgagctatga gaaagcgcca
     2521 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2581 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2641 gccacctctg acttgagcgt ctatttttgt gatgctcgtc aggggggcgg agcctatgga
     2701 aaaacgcctg ctacgtggcc ttcttcctgt tcctggtctt ttgctcacat gttctttccg
     2761 gccttatccc ctgattctgt ggataactgt gttaccgttt ttgtgtgagt cagtaccgct
     2821 cgccgcagtc gaacgaccga gcgtagcgag tcagtgagcg aggaagcgga aaagcgcctg
     2881 gacgtgcatt ttctccttac gcatctgtgc ggcatttcac acccggcatg gcgtactttt
     2941 catacaatcc gcactgatgc cgcatggtta agccagtata cactccgcta tcgctacgtg
     3001 actgggtcag ggctgcgccc cgacacccgc taaaacctgc tgacgcgccc tgacgggctt
     3061 gtcagctccc ggcatccgct cacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     3121 agaggttttc accgtcatcc ccgaaacgtg cgaggcagct gcggtaaagc tcatcggcgt
     3181 ggtcgtgaag cgattcacaa atatcggcct gttcatctgc gtccagttcg ttgagcttct
     3241 ccagcagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct
     3301 gtttagtcac tgatgcctcc gtgtaagggg gatttctgtt catggggtaa tgataccgat
     3361 gaaacgcgag aggatgctca caatacgggt tactgatgat gaacatgccc ggttacttga
     3421 acgctgtgag ggtaaacaac tggcggtatg gatgcggcgg gtctgcctgg gggagccggt
     3481 tgcccgttcc ggaaaactgc cgacactggc accgccgtta ctgcgtcagc tggccgccat
     3541 cggaaataac ctgaatcaga cagcccgtaa ggtgaacagc gggcagtggt cttccggtga
     3601 ccgggttcag gtggtggccg cactgatggc catcggggat gagctgcgcc ggctgcgtct
     3661 ggctgtcagg gaacaggggg cgcgggatga tagttaaatt tcatgccagg ggaaaaggtg
     3721 gtggcagtgg tccggttgat tacctgctgg ggagggagcg taaccgcgaa ggcgcaacgg
     3781 tgcttcaggg taatccggaa gaagtccggg aactcatcga tgccacgcca tttgcgaaga
     3841 aatacacgtc cggtgttctg tcgttcgcgg agaaggagct gccgccggga ggacgtgaaa
     3901 aagtgatggc gagctttgag cgtgtactga tgcccggtct cgaaaaaaat cagtacagca
     3961 tcctgtgggt ggagcaccag gacaagggac ggcttgagct gaattttgtc attccgaaca
     4021 tggagctaca gaccggaaaa cgcctccagc cgtactacga ccgcgcagac aggcctagaa
     4081 ttgatgcctg gcagacgctg gtgaatcacc attacgggct gcatgacccg aacgccccgg
     4141 agaaccgcag gacgttgaca ctccctgata acctgcctga aacgaaacag gcgcttgctg
     4201 agggcgtcac gcgaggtata gatgcacttt accatgccgg agagataaaa ggccgtcagg
     4261 atgtgattca ggcgctcact gaggcggggc tggaagtggt cagggtgacg cgaagcagta
     4321 tcagcattgc agatccgaac ggcgggaaga atatcaggct gaaaggagca ttttatgagc
     4381 aatcttttgc agacgggcgc ggagttcgag aaaaagctga aagagagagc cgaatctaca
     4441 gagaaaatgc tgaacaacga gttcaggagg ctcggcgaat ctgtaagcga ggctgtgaca
     4501 tcaaacgaga cgaaaatcag agacgctata gccctgttca cagcctcgac agaggaatcg
     4561 ctggaaaaac accgggaagg ggtgaaagag gcgatgatgc agcacaggag ggacgtgtta
     4621 aagctggcag ggaatacggg catgatgtta ctggggatag tctttctcct gtttaccgcg
     4681 agtggcggga cgctctggta tcttggaggg aggatacagg cgaacctgga agaaatcagg
     4741 aagcaggaag agacattgca gaaactgaac gcgaagacat ggggcgtgga gtttgtgcag
     4801 gacgggaaca ggaaattcct tgtccttccg tacgggaaat cagcggaggt gattcccttt
     4861 caggggaaag agtgggtaca tctgaaggag tgacacagag tgacagagct ggaaacacat
     4921 ttgctgaacg ccttagagca gctgcaacag gactatatgc agcggctgag cgaatgggag
     4981 agcgccttcg tggaattgca gaagatgttt tcgcttacgc aacgggacaa cgcgatgctg
     5041 aacgagcggg tcatgcagtt gagtcagcag gtgcagcact tgagcgagca gacagaacgc
     5101 ttgagcgagc agacagaacg cttgagccag ttatacagcg agaactggag ataagagagg
     5161 aacggctgat acaggagcgc gaacatgtgt tatccctgga acgggagcgt cagccggaaa
     5221 tacaggaacg cacgctggat ggcccttcgc tgggatggtg aaaccatgaa aaatggcagc
     5281 ttcagtggat taagtggggg taatgtggcc tgtaccctct ggttgcatag gtattcatac
     5341 ggttaaaatt tatcaggcgc gatcgcggca gtttttcggg tggtttgttg ccatttttac
     5401 ctgtctgctg ccgtgatcgc gctgaacgcg ttttagcggt gcgtacaatt aagggattat
     5461 ggtaaatcca cttactgtct gccctcgtag ccatcgagat aaaccgcaga aatcgtgtca
     5521 gccagcagcc tggatttttt cggggtaagg tctttgctgc ggtaatcagt acccgcaaat
     5581 tgagttgtgc cccggagtga gtttaaaaat tcctggctgt gaaactgctg aatgcgttgt
     5641 tgcacatcac tccgtgattt tcctgtcgct ttatctttag ctcccatatc ccagaactgc
     5701 cgaaaaaaag gcaccatcat tcgtgttgtg tcggcttttt gggtggaaaa gttgggatca
     5761 aaaccgtcat cagaagcata tacgtaatgc cgggtatgtt gttccacgct gccctttggg
     5821 gtacaggctg tgagagctac tgttgctgtt atcatggcga taagacaaag tttgtttccg
     5881 gtacgtctca tgagtgtctc cctaccttaa agtatttatt ctcggtgagt tattatccga
     5941 agactatttt atcatcagtc tctgacagcg ttcagcgacc gaacaacctg gaccagaatc
     6001 cccgtttctt ttccagttcc agtgcctgcc tgagctgggc tatctcattt tgtagctgtt
     6061 cccgttctgc ttcctggcgt ctgcgatcca tatcctgtgc ctgtttatcc tcaagcatca
     6121 gcgtcaggca ttgtttcagc tcattcagct cccggagaat gcgttctgtc tgctgatcat
     6181 gtggattttc tgcatgtccc tcactgtgcc tttctggtgt ctcattctgc tttaattcgc
     6241 cgtatgcccg gatcagttca ctggtttcaa actcccgtcg tccgtccctg ccggtacggt
     6301 aactgacaag gcctgcgcgc atatcacggt aaaactgtga acgcgatctg cctgtcattt
     6361 ttagtgcgtc ccggagtgtg tgccatgcca taaagtgaca gtgtcccata gatgtctcat
     6421 ctcatagttt cagtaaaaca taatgaggtc tgagaacggt aatgtttgtg ctggtttttg
     6481 tggcatcggg cgagaatagc gcgtggtgtg aaagactgtt tttttgatcg ttttcacaaa
     6541 aatggaagtc cacagtcttg acagggaaaa tgcagcggcg tagcttttat gctgtatata
     6601 aaaccagtgg ttatatgtac agtatttatt tttaacttat tgttttaaaa gtcaaagagg
     6661 attttata
//
