LOCUS       NZ_CP122628             6647 bp    DNA     circular CON 08-JUN-2024
DEFINITION  Escherichia coli strain ETEC4089 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP122628
VERSION     NZ_CP122628.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32549665
            Assembly: GCF_030013125.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Vereecke,N., Van Hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Virotyping and genetic antimicrobial susceptibility testing of
            porcine ETEC/STEC strains and associated plasmid types
  JOURNAL   Front Microbiol 14, 1139312 (2023)
   PUBMED   37143544
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Vereecke,N., Van hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2023) Department of Pathobiology, Pharmacology and
            Zoological Medicine, Universiteit Gent, Salisburylaan 133, Merelbeke
            9820, Belgium
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Trycycler; minimap2; medaka v. v0.5.3; v2.20;
            v1.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 138x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030013125.1-RS_2024_06_08
            Annotation Date                   :: 06/08/2024 04:45:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,755
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,410
            CDSs (with protein)               :: 4,410
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 77 of 224
            Pseudo Genes (incomplete)         :: 145 of 224
            Pseudo Genes (internal stop)      :: 44 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP122628.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ETEC4089"
                     /isolation_source="feces"
                     /host="Sus scrofa"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="Belgium"
                     /collection_date="2012"
                     /collected_by="IMM_DI04"
     gene            1..138
                     /locus_tag="QDW64_RS23710"
                     /old_locus_tag="QDW64_23705"
     CDS             1..138
                     /locus_tag="QDW64_RS23710"
                     /old_locus_tag="QDW64_23705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(337..495)
                     /locus_tag="QDW64_RS23815"
     CDS             complement(337..495)
                     /locus_tag="QDW64_RS23815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(1165..1356)
                     /locus_tag="QDW64_RS23715"
                     /old_locus_tag="QDW64_23710"
     CDS             complement(1165..1356)
                     /locus_tag="QDW64_RS23715"
                     /old_locus_tag="QDW64_23710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1420..1743
                     /locus_tag="QDW64_RS23720"
                     /old_locus_tag="QDW64_23715"
     CDS             1420..1743
                     /locus_tag="QDW64_RS23720"
                     /old_locus_tag="QDW64_23715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1952..>2383
                     /locus_tag="QDW64_RS23725"
                     /old_locus_tag="QDW64_23720"
                     /pseudo=""
     CDS             1952..>2383
                     /locus_tag="QDW64_RS23725"
                     /old_locus_tag="QDW64_23720"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2421..2939
                     /locus_tag="QDW64_RS23730"
                     /old_locus_tag="QDW64_23725"
     CDS             2421..2939
                     /locus_tag="QDW64_RS23730"
                     /old_locus_tag="QDW64_23725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            2946..3179
                     /locus_tag="QDW64_RS23735"
                     /old_locus_tag="QDW64_23730"
     CDS             2946..3179
                     /locus_tag="QDW64_RS23735"
                     /old_locus_tag="QDW64_23730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3497..3916)
                     /locus_tag="QDW64_RS23740"
                     /old_locus_tag="QDW64_23735"
     CDS             complement(3497..3916)
                     /locus_tag="QDW64_RS23740"
                     /old_locus_tag="QDW64_23735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(3997..4434)
                     /locus_tag="QDW64_RS23745"
                     /old_locus_tag="QDW64_23740"
     CDS             complement(3997..4434)
                     /locus_tag="QDW64_RS23745"
                     /old_locus_tag="QDW64_23740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            4694..6262
                     /gene="cea"
                     /locus_tag="QDW64_RS23750"
                     /old_locus_tag="QDW64_23745"
     CDS             4694..6262
                     /gene="cea"
                     /locus_tag="QDW64_RS23750"
                     /old_locus_tag="QDW64_23745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            complement(6259..6600)
                     /locus_tag="QDW64_RS23755"
                     /old_locus_tag="QDW64_23750"
     CDS             complement(6259..6600)
                     /locus_tag="QDW64_RS23755"
                     /old_locus_tag="QDW64_23750"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_000058759.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNSEGYYFLVSDKMLYAIVI
                     STILCPYSKYAIEHIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 immunity protein"
     Typing          993..1080
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(424..979)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.878"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          1734..3283
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgaggaaaa gattttttgt gggaatattc gcgataaacc tccttgttgg atgtcaggct
       61 aactatatac gtgatgttca gggagggacc atcgcaccat cctcctcttc taaactgacg
      121 gggatcgcgg ttcagtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
      181 taatctgctg cttgcaaaca aaaaaaccac cgctaccaac ggtggtttgt ttgccggatc
      241 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
      301 ctgtccttct agtgtagccg tagtcgggcc actacttcaa gaactctgta gcaccgtttg
      361 tgccatcatc gctctgctaa tccggttacc agtggctgct gccagtggcg ttaaggcgtg
      421 ccttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
      481 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacca
      541 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
      601 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
      661 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
      721 ctcgtcaggg gggcggagcc tatggaaaaa cgcctgctac gtggccttct tcctgttcct
      781 ggtcttttgc tcacatgttc tttccggcct tatcccctga ttctgtggat aactgtgtta
      841 ccgtttttgt gtgagtcagt accgctcgcc gcagtcgaac gaccgagcgt agcgagtcag
      901 tgagcgagga agcggaaaag cgcctggacg tgcattttct ccttacgcat ctgtgcggca
      961 tttcacaccc ggcatggcgt acttttcata caatccgcac tgatgccgca tggttaagcc
     1021 agtatacact ccgctatcgc tacgtgactg ggtcagggct gcgccccgac acccgctaaa
     1081 acctgctgac gcgccctgac gggcttgtca gctcccggca tccgctcaca gacaagctgt
     1141 gaccgtctcc gggagctgca tgtatcagag gttttcaccg tcatccccga aacgtgcgag
     1201 gcagctgcgg taaagctcat cggcgtggtc gtgaagcgat tcacaaatat cggcctgttc
     1261 atctgcgtcc agttcgttga gcttctccag cagcgttaat gtctggcttc tgataaagcg
     1321 ggccatgtta agggcggttt tttcctgttt agtcactgat gcctccgtgt aagggggatt
     1381 tctgttcatg gggtaatgat accgatgaaa cgcgagagga tgctcacaat acgggttact
     1441 gatgatgaac atgcccggtt acttgaacgc tgtgagggta aacaactggc ggtatggatg
     1501 cggcgggtct gcctggggga gccggttgcc cgttccggaa aactgccgac actggcaccg
     1561 ccgttactgc gtcagctggc cgccatcgga aataacctga atcagacagc ccgtaaggtg
     1621 aacagcgggc agtggtcttc cggtgaccgg gttcaggtgg tggccgcact gatggccatc
     1681 ggggatgagc tgcgccggct gcgtctggcc gtcagggaac agggggcgcg ggatgatagt
     1741 taaatttcat gccaggggaa aaggtggtgg cagtggtccg gttgattacc tgctggggag
     1801 ggagcgtaac cgcgaaggcg caacggtgct tcagggtaat ccggaagaag tccgggaact
     1861 catcgatgcc acgccatttg cgaagaaata cacgtccggt gttctgtcgt tcgcggagaa
     1921 ggagctgccg ccgggaggac gtgaaaaagt gatggcgagc tttgagcgtg tactgatgcc
     1981 cggtctcgaa aaaaatcagt acagcatcct gtgggtggag caccaggaca agggacggct
     2041 tgagctgaat tttgtcattc cgaacatgga gctacagacc ggaaaacgcc tccagccgta
     2101 ctacgaccgc gcagacaggc ctagaattga tgcctggcag acgctggtga atcaccatta
     2161 cgggctgcat gacccgaacg ccccggagaa ccgcaggacg ctgacactcc ctgataacct
     2221 gcctgaaacg aaacaggcgc ttgctgaggg cgtcacgcga ggtatagatg cactttacca
     2281 tgccggagag ataaaaggcc gtcaggatgt gattcaggcg ctcactgagg cggggctgga
     2341 agtggtcagg gtgacgcgaa gcagtatcag cattgcagat ccgaacggcg ggaagaatat
     2401 caggctgaaa ggagcatttt atgagcaatc ttttgcagac gggcgcggag ttcgagaaaa
     2461 agctgaaaga gagagccgaa tctacagaga aaatgctgaa caacgagttc aggaggctcg
     2521 gcgaatctgt aagcgaggct gtgacatcaa acgagacgaa aatcagagac gctatagccc
     2581 tgttcacagc ctcgacagag gaatcgctgg aaaaacaccg ggaaggggtg aaagaggcga
     2641 tgatgcagca caggagggac gtgttaaagc tggcagggaa tacgggcatg atgttactgg
     2701 ggatagtctt tctcctgttt accgcgagtg gcgggacgct ctggtatctt ggagggagga
     2761 tacaggcgaa cctggaagaa atcaggaagc aggaagagac attgcagaaa ctgaacgcga
     2821 agacatgggg cgtggagttt gtgcaggacg ggaacaggaa attccttgtc cttccgtacg
     2881 ggaaatcagc ggaggtgatt ccctttcagg ggaaagagtg ggtacatctg aaggagtgac
     2941 acagagtgac agagctggaa acacatttgc tgaacgcctt agagcagctg caacaggact
     3001 atatgcagcg gctgagcgaa tgggagagcg ccttcgtgga attgcagaag atgttttcgc
     3061 ttacgcaacg ggacaacgcg atgctgaacg agcgggtcat gcagttgagt cagcaggtgc
     3121 agcacttgag cgagcagaca gaacgcttga gccagttata cagcgagaac tggagataag
     3181 agaggaacgg ctgatacagg agcgcgaaca tgtgttatcc ctggaacggg agcgtcagcc
     3241 ggaaatacag gaacgcacgc tggatggccc ttcgctggga tggtgaaacc atgaaaaatg
     3301 gcagcttcag tggattaagt gggggtaatg tggcctgtac cctctggttg cataggtatt
     3361 catacggtta aaatttatca ggcgcgatcg cggcagtttt tcgggtggtt tgttgccatt
     3421 tttacctgtc tgctgccgtg atcgcgctga acgcgtttta gcggtgcgta caattaaggg
     3481 attatggtaa atccacttac tgtctgccct cgtagccatc gagataaacc gcagaaatcg
     3541 tgtcagccag cagcctggat tttttcgggg taaggtcttt gctgcggtaa tcagtacccg
     3601 caaattgagt tgtgccccgg agtgagttta aaaattcctg gctgtgaaac tgctgaatgc
     3661 gttgttgcac atcactccgt gattttcctg tcgctttatc tttagctccc atatcccaga
     3721 actgccgaaa aaaaggcacc atcattcgtg ttgtgtcggc tttttgggtg gaaaagttgg
     3781 gatcaaaacc gtcatcagaa gcatatacgt aatgccgggt atgttgttcc acgctgccct
     3841 ttggggtaca ggctgtgaga gctactgttg ctgttatcat ggcgataaga caaagtttgt
     3901 ttccggtacg tctcatgagt gtctccctac cttaaagtat ttattctcgg tgagttatta
     3961 tccgaagact attttatcat cagtctctga cagcgttcag cgaccgaaca acctggacca
     4021 gaatccccgt ttcttttcca gttccagtgc ctgcctgagc tgggctatct cattttgtag
     4081 ctgttcccgt tctgcttcct ggcgtctgcg atccatatcc tgtgcctgtt tatcctcaag
     4141 catcagcgtc aggcattgtt tcagctcatt cagctcccgg agaatgcgtt ctgtctgctg
     4201 atcatgtgga ttttctgcat gtccctcact gtgcctttct ggtgtctcat tctgctttaa
     4261 ttcgccgtat gcccggatca gttcactggt ttcaaactcc cgtcgtccgt ccctgccggt
     4321 acggtaactg acaaggcctg cgcgcatatc acggtaaaac tgtgaacgcg atctgcctgt
     4381 catttttagt gcgtcccgga gtgtgtgcca tgccataaag tgacagtgtc ccatagatgt
     4441 ctcatctcat agtttcagta aaacataatg aggtctgaga acggtaatgt ttgtgctggt
     4501 ttttgtggca tcgggcgaga atagcgcgtg gtgtgaaaga ctgttttttt gatcgttttc
     4561 acaaaaatgg aagtccacag tcttgacagg gaaaatgcag cggcgtagct tttatgctgt
     4621 atataaaacc agtggttata tgtacagtat ttatttttaa cttattgttt taaaagtcaa
     4681 agaggatttt ataatggaaa ccgcggtagc gtactataaa gatggtgttc cttatgatga
     4741 taagggacag gtaattatta ctcttttgaa tggtactcct gacgggagtg gctctggcgg
     4801 cggaggtgga aaaggaggca gtaaaagtga aagttctgca gctattcatg caactgctaa
     4861 atggtctact gctcaattaa agaaaacaca ggcagagcag gctgcccggg caaaagctgc
     4921 agcggaagca caggcgaaag caaaggcaaa cagggatgcg ctgactcagc gcctgaagga
     4981 tatcgtgaat gaggctcttc gtcacaatgc ctcacgtacg ccttcagcaa cagagcttgc
     5041 tcatgctaat aatgcagcta tgcaggcgga agcagagcgt ttgcgccttg cgaaagcaga
     5101 agaaaaagcc cgtaaagaag cggaagcagc agaaaaggct tttcaggaag cagaacaacg
     5161 acgtaaagag attgaacggg agaaggctga aacagaacgc cagttgaaac tggctgaagc
     5221 tgaagagaaa cgactggctg cattgagtga agaagctaaa gctgttgaga tcgcccaaaa
     5281 aaaactttct gctgcacaat ctgaagtggt gaaaatggat ggagagatta agactctcaa
     5341 ttctcgttta agctccagta tccatgcccg tgatgcagaa atgaaaacgc tcgctggaaa
     5401 acgaaatgaa ctggctcagg catccgctaa atataaagaa ctggatgagc tggtcaaaaa
     5461 actatcacca agagccaatg atccgcttca gaaccgtcct ttttttgaag caaccagacg
     5521 acgggttggg gccggtaaga ttagagaaga aaaacaaaaa caggtaacag catcagaaac
     5581 acgtattaac cggataaatg ctgatataac tcagatccag aaggctattt ctcaggtcag
     5641 taataatcgt aatgccggta tcgctcgtgt tcatgaagct gaagaaaatt tgaaaaaagc
     5701 acagaataat ctccttaatt cacagattaa ggatgctgtt gatgcaacag ttagctttta
     5761 tcaaacgctg actgaaaaat atggtgaaaa atattcgaaa atggcacagg aacttgctga
     5821 taagtctaaa ggtaagaaaa tcggcaatgt gaatgaagct ctcgctgctt ttgaaaaata
     5881 caaggatgtt ttaaataaga aattcagcaa agccgatcgt gatgctattt ttaatgcgtt
     5941 ggcatcggtg aagtatgatg actgggctaa acatttagat cagtttgcca agtacttgaa
     6001 gattacgggg catgtttctt ttggatatga tgtggtatct gatatcctaa aaattaagga
     6061 tacaggtgac tggaagccac tatttcttac attagagaag aaagctgcag atgcaggggt
     6121 gagttatgtt gttgctttac tttttagctt gcttgctgga actacattag gtatttgggg
     6181 tattgctatt gttacaggaa ttctatgctc ctatattgat aagaataaac ttaatactat
     6241 aaatgaggtg ttagggattt aattattctt tattgatata aaaagtccta gcaatccaaa
     6301 tgggattgct aggaccaaac aaagtagatt atatagcata aataggttta attttgctac
     6361 gggggcgtta tttaggtttt ttcttctttc gaaaaaatct ttctttatga agttaaaagc
     6421 tatgtgttca atagcatatt ttgaatatgg acatagaata gtgcttatca ctattgcata
     6481 tagcatctta tctgacacaa ggaaataata cccttcgctg ttttttgtta taaggtatat
     6541 atatataagt gtgcagtaca ggccaaataa aatatttttt atgtagtatc ttaagctcat
     6601 aaattaaacc tcgccatata ttcttttcat tttataagga tcgagtt
//
