LOCUS       NZ_CP122627            24405 bp    DNA     circular CON 08-JUN-2024
DEFINITION  Escherichia coli strain ETEC4089 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP122627
VERSION     NZ_CP122627.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32549665
            Assembly: GCF_030013125.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24405)
  AUTHORS   Vereecke,N., Van Hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Virotyping and genetic antimicrobial susceptibility testing of
            porcine ETEC/STEC strains and associated plasmid types
  JOURNAL   Front Microbiol 14, 1139312 (2023)
   PUBMED   37143544
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24405)
  AUTHORS   Vereecke,N., Van hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2023) Department of Pathobiology, Pharmacology and
            Zoological Medicine, Universiteit Gent, Salisburylaan 133, Merelbeke
            9820, Belgium
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Trycycler; minimap2; medaka v. v0.5.3; v2.20;
            v1.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 138x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030013125.1-RS_2024_06_08
            Annotation Date                   :: 06/08/2024 04:45:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,755
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,410
            CDSs (with protein)               :: 4,410
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 77 of 224
            Pseudo Genes (incomplete)         :: 145 of 224
            Pseudo Genes (internal stop)      :: 44 of 224
            Pseudo Genes (multiple problems)  :: 39 of 224
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP122627.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..24405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ETEC4089"
                     /isolation_source="feces"
                     /host="Sus scrofa"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="Belgium"
                     /collection_date="2012"
                     /collected_by="IMM_DI04"
     gene            1..2745
                     /locus_tag="QDW64_RS23530"
                     /old_locus_tag="QDW64_23525"
     CDS             1..2745
                     /locus_tag="QDW64_RS23530"
                     /old_locus_tag="QDW64_23525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="WP_001096401.1"
                     /translation="MAKLLKAMKRPAALWGVPMVPLLTVVGVTIIVAIWTSIALLLFLP
                     IEFLVMKSLTRNEPLRFNLIAVWLRVRGNPVANRLFGATAFMPVEHNAVDIQEFIDAMK
                     LNQCATIKKYIPYSSHIHPNVVRSLNSDFFCTWELTGTPFDCESDETLQFGTNQLHGLI
                     RSFEGFPVTFYVHNSREQFTDSLHKVSGNPYADTVSERYYASLNKRPFRRNRLFVTICY
                     RPFASLDKANRKRMKDGQKLKEIDSALLEVLEIKSTLDTALSRYGAHALGTFTENEAVF
                     SSQLAFYEYLLTHQWRKVRVTRTPAYNVMGNVALYFSAESGQINHVNGTQYFRGLEVKE
                     FSEETATGMTDSLLYAPCDYVITQSYTCMSREEAKKAIKRTRRLMMSADDDAVSQRLDL
                     DVALDLLTSGKIAYGKHHLSIMVFSPSLESLIADTNEISNALNNIGITPVPAEISLSAA
                     YMAQLPGNYNLRPRAGELSSLNFVELASLHNFYPGKRDKAPWGDAMALVRTPSGDGYYL
                     NLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFLQINQQKYNRDDSFSPDARTKRL
                     TTVYLDKDRGAEINIRALGGKYYRVFSGEPTGWNPFALPATKRNINFIKQLMKILCTRN
                     GATITPRDERRLNDAVNAVMSDEPQYRVYGITRMLENLPEPATREAQENGLSIRLSQWA
                     RGGEFGWVFDNEHDTFDISNCDNFGIDGTEFLDDPNVCAPISFYLLYRITSLLDGRRLV
                     IFMDEFWKWLRDPVFKDFAYNKLKTIRKLNGMLVVGTQSPAEIIKDEIAPAVIEQCGTQ
                     ILAANPSADRSHYVDGMKFEPEVFDVIKGLDPQARQYVVVKNQFRRGDTKRFAARITLD
                     LSGIGRYTKVMSGDAPNLEIFESLYQEGMKPSDWLDSYLAKAL"
                     /name="VirB3 family type IV secretion system protein"
     gene            2760..3464
                     /locus_tag="QDW64_RS23535"
                     /old_locus_tag="QDW64_23530"
     CDS             2760..3464
                     /locus_tag="QDW64_RS23535"
                     /old_locus_tag="QDW64_23530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010617328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000819778.1"
                     /translation="MKRFRPLILAVLIASPIVNAAGIPVFDATARLENIRQWTKEANQW
                     LQTVQHYKDQLQAYQKQLETVTGIRDIQAFVNQAKSLKADLDKLRKPGQALNDLLLSGG
                     SSGQFDELYNKYKIFDNCNAEQSKSYANTCRQEVINKAIQIEQTNEVQTQIGEMLGEIN
                     ELSDRVANAKDMKEAQDLANSIQLKSVMLNTLTTQWEMNVKAAEKREKMLADERRKKWN
                     QQQLNAPEIMFN"
                     /name="type IV secretion system protein"
     gene            3476..3703
                     /locus_tag="QDW64_RS23540"
                     /old_locus_tag="QDW64_23535"
     CDS             3476..3703
                     /locus_tag="QDW64_RS23540"
                     /old_locus_tag="QDW64_23535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010617327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="WP_000720509.1"
                     /translation="MKKIILFTIISGLMALTSGCKEEAKTTKWYREHPDELKVVYAECQ
                     KTGSASENCKHANEAHWQIQQLNAPEISFN"
                     /name="EexN family lipoprotein"
     gene            3713..4783
                     /locus_tag="QDW64_RS23545"
                     /old_locus_tag="QDW64_23540"
     CDS             3713..4783
                     /locus_tag="QDW64_RS23545"
                     /old_locus_tag="QDW64_23540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_024187435.1"
                     /translation="MMAGIFSSVEKNIIGGITDATNGLMMSYSSMMMGLAATSATIYIM
                     WRGYQTLAGKLSTPMEDVMWDIMRMAIILSFVANINGYLDGVIDAVNGIKEGFSSSTSI
                     WQLLDTLWNSARVLGKTLHDLDNSEFIKEEGTIAELFVWGGVFVLMIITAFVSMIAEIM
                     ILLLGITAPIFIFCLMYGFLRPMFNNWLQNIFAAILTIMFSALALRIVIGFLTSRMSEA
                     VTQASKANIVELGAQVCLAGVCAAILVYMSAKLAGALAGASATATMQGAAALGIGAAAY
                     GAGKMLKEAGKVLPKEVASPSNKGLGDPQSRIVFNAADARKHAIEQMIARNEARMLTDQ
                     SAPKLPPSIKRLPPPR"
                     /name="type IV secretion system protein"
     gene            4833..4976
                     /locus_tag="QDW64_RS23550"
                     /old_locus_tag="QDW64_23545"
     CDS             4833..4976
                     /locus_tag="QDW64_RS23550"
                     /old_locus_tag="QDW64_23545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000825480.1"
                     /translation="MKRLIALALLCVLSGCAQKSKSLPHAFSGQGSPVNTPQIIAELTT
                     HD"
                     /name="hypothetical protein"
     gene            4969..5652
                     /locus_tag="QDW64_RS23555"
                     /old_locus_tag="QDW64_23550"
     CDS             4969..5652
                     /locus_tag="QDW64_RS23555"
                     /old_locus_tag="QDW64_23550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000139290.1"
                     /translation="MTEQKLIADSRSFEQQMIDRDKRATKAGFLVGGAGLLITILSLIA
                     IVIMLPLKQTTVELYTVDNHTGRVEHVTRASKTALTAEEAYQKAMAANYVKLRERYVYP
                     ALQDDYETVQIYNSPQVNDDYLALYAGKNAPDKVYNNAAHTVKIEIISEQITNATAPDK
                     VATVRYKKIIRRMADNSIRNEYWDARFTFHSDPEKEMSDVEREINPFGFTVTSWQTDRE
                     IRGGE"
                     /name="type IV secretion system protein"
     gene            5652..6557
                     /gene="virB9"
                     /locus_tag="QDW64_RS23560"
                     /old_locus_tag="QDW64_23555"
     CDS             5652..6557
                     /gene="virB9"
                     /locus_tag="QDW64_RS23560"
                     /old_locus_tag="QDW64_23555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="WP_001230135.1"
                     /translation="MRKLTLITLIIMLNSQAWAAATPQASRYDARIQQVIYNPQNVTVV
                     NTKPGFMTTLVFDNDEAVISASPGFDEAWEARADANRVNIRPVALTQGAPGEDGNTTQV
                     VIPPNSRDWHTNMLIVTNKRLYNVELNVIDEKSPQQPAYLVSYRYPGDERNRANKEAAA
                     RQKEWEQKQEQTQIQKALRTAQTPRNWDYTKYPGKDSYNIEPDFAYDDGRFTFLGFSPN
                     KSVPSVTKEQNGQEYVVNSSIQRKGNFTVLVIQEITPRLVLRSGDAVVGLKNNGFGKVH
                     AADNSTVSDKVERVEKTDNT"
                     /name="virB9"
     gene            6599..7864
                     /gene="virB10"
                     /locus_tag="QDW64_RS23565"
                     /old_locus_tag="QDW64_23560"
     CDS             6599..7864
                     /gene="virB10"
                     /locus_tag="QDW64_RS23565"
                     /old_locus_tag="QDW64_23560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB10/TraB/TrbI family type IV secretion system
                     protein"
                     /protein_id="WP_000129037.1"
                     /translation="MTDENKSPSTEEEKPVMTSDQDDIATLEREARAKREAELQASQDD
                     EKENSLQPAVNKLKKRQKGKAAAFLAICAVALIGLTWGGNWVYQNFLKQENGEKTKEVT
                     PPKKTNDYRKRTDLGMATNEPENSTEGEQAVSQDNTGNVSGTAGQMKTFEPPQLNKASF
                     LVRRNNTASPQNQVKTRQQEMILAAENNATQPTDSTATTTQHGTASQAISPATVRRIPY
                     NPDLYIPENTVIPCSMDYRFVSDRAGKIRCTVAKDIWSASGNTKLIEKGTTATGIYQTG
                     ITQGQGRAFIMMTKLRTRQRPYLDIPLIDTNAAGELGESGVDGWIDTHFWERFGGALMV
                     GMIPDIGAWASNNAGKKDRNTDYTENSRQAMADMARTTLENSINIPPTLYKNQGEIINL
                     ITGQDIDFSNIYTLRLKNELQR"
                     /name="virB10"
     gene            7848..8039
                     /locus_tag="QDW64_RS23570"
                     /old_locus_tag="QDW64_23565"
     CDS             7848..8039
                     /locus_tag="QDW64_RS23570"
                     /old_locus_tag="QDW64_23565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000995652.1"
                     /translation="MNCKDKETWLHMLAAGHMGGLAGNPNEAAAQDAYQRLIKNKTAQA
                     VLQRCTTRITLKGANDDR"
                     /name="hypothetical protein"
     gene            8029..9054
                     /gene="virB11"
                     /locus_tag="QDW64_RS23575"
                     /old_locus_tag="QDW64_23570"
     CDS             8029..9054
                     /gene="virB11"
                     /locus_tag="QDW64_RS23575"
                     /old_locus_tag="QDW64_23570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125556.1"
                     /GO_component="GO:0043684 - type IV secretion system
                     complex [Evidence IEA]"
                     /GO_process="GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="WP_000125556.1"
                     /translation="MTAKNISLDRYKQRFFGDFLELPGLTEIAVNRPGELWTKIDGVWG
                     QHTVDLKFDDCYNFARCLAKHHNDNIEDIKPGLSATLESGERCQVVVPPACERDTISIT
                     IRKPSKIQIPHQAYIDSGFYNRVTGIEKASTHNEELIELYKSKNIPLFMEKCVEYGKTL
                     AVAGETSTGKTTYMKMLIQYIPVHLRITTIEDNPEIIFYKHKNYVHLFYPSESSDEKGS
                     VVTPAALIRWNYRMNPDRILLTEVRGGEAWDFLKITGSGHEGSMTSIHAGSAHEAIDGF
                     ITRCTENPQCAQLPYSFLLRKVLDSLDVVVSIDLDGNVRRMNDIYFRPLHRNQYYEEMK
                     V"
                     /name="virB11"
     gene            9051..9449
                     /locus_tag="QDW64_RS23580"
                     /old_locus_tag="QDW64_23575"
     CDS             9051..9449
                     /locus_tag="QDW64_RS23580"
                     /old_locus_tag="QDW64_23575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cag pathogenicity island Cag12 family protein"
                     /protein_id="WP_021533590.1"
                     /translation="MIKHILVTVLVTLITGCSSPPPPVPVEWNKKPVSLNEKLPEWNPN
                     SLIIKSPVINDKWTLSISSQDFNKEDWMPSVFYAIAHSTQITVATKSGADYFSAKNWLY
                     HHGANGVIIYHPITNCLTCNETTIYFSR"
                     /name="cag pathogenicity island Cag12 family protein"
     gene            9478..9777
                     /locus_tag="QDW64_RS23585"
                     /old_locus_tag="QDW64_23580"
     CDS             9478..9777
                     /locus_tag="QDW64_RS23585"
                     /old_locus_tag="QDW64_23580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="WP_000716758.1"
                     /translation="MKKIAAITMFVLTTATPVYSYAADPCEVVLCMYGKATGNSGGGEC
                     RSAEKAFFNILKKKKGSIRWSKTFDARKAFLNQCSSADPAAISKIMSKFGRVRG"
                     /name="TrbM/KikA/MpfK family conjugal transfer protein"
     gene            9780..9995
                     /locus_tag="QDW64_RS23590"
                     /old_locus_tag="QDW64_23585"
     CDS             9780..9995
                     /locus_tag="QDW64_RS23590"
                     /old_locus_tag="QDW64_23585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001090643.1"
                     /translation="MNTDENMVFYLTNSDLVYSYAHKIVCSIPSGMKGNFITSIGETLE
                     DLKKRYPDIILITESEALALQSKREN"
                     /name="hypothetical protein"
     gene            10023..10328
                     /locus_tag="QDW64_RS23595"
                     /old_locus_tag="QDW64_23590"
     CDS             10023..10328
                     /locus_tag="QDW64_RS23595"
                     /old_locus_tag="QDW64_23590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000910475.1"
                     /translation="MLKEDMFINNIHSRNQDRIHVKRVFDDISNKANRGCGLYYEIYQA
                     RLMRGLIEHTVKLKSSDANKLISYAASQGYEITEDRYNAAIKAEDECLTEITRAQV"
                     /name="hypothetical protein"
     gene            11138..12865
                     /locus_tag="QDW64_RS23600"
                     /old_locus_tag="QDW64_23595"
     CDS             11138..12865
                     /locus_tag="QDW64_RS23600"
                     /old_locus_tag="QDW64_23595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615560.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA transfer
                     family protein"
                     /protein_id="WP_001068080.1"
                     /translation="MNPHLMQKLNGIADAFSIFLLWLQNSPVILSLLAGLTLPFIFNLP
                     REERKNAPFWLKSVACVSIFFFIFGTMSPLSIHGLSYLFDVLDPNTLLRIPLWIITILF
                     TLAGLAFHLTARRLLAGEIDSLKHRMIKKSKLERNTRTDVRKVKELLPDSIEYNPLDYI
                     DLKKGVFIGLDKDDQPQYITIKEFQKQHAAIIGTTGSGKGITASVLLYQAILLGEAVFV
                     EDPKDDEWAVHVLREACKKAGKKFVVINLNRTNHQLDLLADISHEQLEELFNAGFSLSK
                     KGEAADFYRIADRRAARNVAAIHKTGMTLYDLFNDDNVKSIGEDAPAFLGELEEIALVN
                     SVNAKNGFSLKEIFDNGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINTTPRVV
                     AIFLDELKYHISRPTMEGLGTARDKSVHIFMAFQAIDDLRDCPSDLNGDSVIGAVIENA
                     KFKLIYKIQNPETAEWAAKMTGSILVDDEMRKVRTDLSLTEKVDTDRMIRQAESYYVDS
                     NMFLNLPERVGFVFTSKDLPKATKMSHIPANKETIELLSFETEQIEQHNNNDVPSSGL"
                     /name="type IV secretory system conjugative DNA transfer
                     family protein"
     gene            12875..13609
                     /gene="mobC"
                     /locus_tag="QDW64_RS23605"
                     /old_locus_tag="QDW64_23600"
     CDS             12875..13609
                     /gene="mobC"
                     /locus_tag="QDW64_RS23605"
                     /old_locus_tag="QDW64_23600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_000905147.1"
                     /translation="MLIHDFYERKRISANNMLCLLNFLKDETYSDFHIIKRKLGFTKKS
                     RNPVYKLLNKALKEGFIIKHEIPSSKRKLTVWGITMQGLAMVVKADDITFPSYFEPSKL
                     KYWTLEHRLLNQRVRISLESKGATEWINGDSAAFIARYSGCSHRPDGVIKQPTGAIMAI
                     ETERTMKTRARYISIINSHLTARDKGNWHYALYVMPDTKSKESLIRLFDSIKTVMRNNT
                     PVPFDAKNREMFIFRTIDELEQ"
                     /name="mobC"
     gene            13613..14077
                     /locus_tag="QDW64_RS23610"
                     /old_locus_tag="QDW64_23605"
     CDS             13613..14077
                     /locus_tag="QDW64_RS23610"
                     /old_locus_tag="QDW64_23605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="WP_000697615.1"
                     /translation="MKILIIFALYILSPSLYANAADIQGKVIRVLDGDTIEILQNKTPV
                     RIRLANIDAPEKKQAYGRWSTDQLKSLIAGQSVRVTYSHNDRYGRIIGRVFTTGGSEAN
                     RFMVQSGAAWVYEHYNTDNALADLQQEARSQKRGLWADPNPVPPWEWRHK"
                     /name="thermonuclease family protein"
     gene            14087..>14392
                     /locus_tag="QDW64_RS23615"
                     /old_locus_tag="QDW64_23610"
                     /pseudo=""
     CDS             14087..>14392
                     /locus_tag="QDW64_RS23615"
                     /old_locus_tag="QDW64_23610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            14571..14834
                     /locus_tag="QDW64_RS23620"
                     /old_locus_tag="QDW64_23615"
     CDS             14571..14834
                     /locus_tag="QDW64_RS23620"
                     /old_locus_tag="QDW64_23615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="WP_001312455.1"
                     /translation="MTIKILVVSNDGHEKLIDLSPDNDLAKITKSLRTAENRMVCVIQD
                     NNRILRWDRSYASRAKNHWRKVAPDRFEILGTVEHIHYVGKC"
                     /name="PilI type IV pilus biogenesis protein"
     gene            complement(14866..15099)
                     /locus_tag="QDW64_RS23625"
                     /old_locus_tag="QDW64_23620"
     CDS             complement(14866..15099)
                     /locus_tag="QDW64_RS23625"
                     /old_locus_tag="QDW64_23620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011998900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000024515.1"
                     /translation="MANVPPKPRNTKGEPPSLEDTQHGFNPTRKNADGRKAQLNLYVDM
                     EVKREFRSIAAANDMKHGELLEAYQEFWKKHH"
                     /name="hypothetical protein"
     gene            complement(15139..15786)
                     /locus_tag="QDW64_RS23630"
                     /old_locus_tag="QDW64_23625"
     CDS             complement(15139..15786)
                     /locus_tag="QDW64_RS23630"
                     /old_locus_tag="QDW64_23625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214917.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_000214917.1"
                     /translation="MTVAIALVNGKGGVKKSSMSRAIATEYTKADWKVLGLDMDVDQGS
                     FLDWGQRRLRYKVEPPVTIQGCGTVSQAKKHIESGIWDLVVIDSAAYATKSLLSLAEIV
                     DLLVLPSGFSVDDLRTTVTTVNALRNKGHSTNKMAVVFVGVSESESEYRTALEYLKPCG
                     VQVINGAIPFMTSFSQAQDKGLALTESPFPGPRQKADEVVQGVIDLLTKLSQ"
                     /name="ParA family protein"
     gene            16152..16502
                     /locus_tag="QDW64_RS23635"
                     /old_locus_tag="QDW64_23630"
     CDS             16152..16502
                     /locus_tag="QDW64_RS23635"
                     /old_locus_tag="QDW64_23630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000269926.1"
                     /translation="MWTVYFGRLFDEWFEEQELALKRKVLAELKHLEEFGPSLSRPHAD
                     TVKGSRHKNMKELRIQYEGHPIRAFFAFDPIRQAIILCAGDKSNDKKFYDRMIRIADEE
                     FSTHLAELEGKK"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            16499..16798
                     /locus_tag="QDW64_RS23640"
                     /old_locus_tag="QDW64_23635"
     CDS             16499..16798
                     /locus_tag="QDW64_RS23640"
                     /old_locus_tag="QDW64_23635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001259443.1"
                     /translation="MRTLEEVIAAQSPESQAIIKAMADEMILETGLQMMREELQLSQKQ
                     VADAMGISQPAVTQLEQRGNDLKLATLKRYVEAMGGKLSLDVELPTGKRVAFNI"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(16829..17074)
                     /locus_tag="QDW64_RS23645"
                     /old_locus_tag="QDW64_23640"
     CDS             complement(16829..17074)
                     /locus_tag="QDW64_RS23645"
                     /old_locus_tag="QDW64_23640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001193619.1"
                     /translation="MQTKPTPADVKEARISAGLTLKEAAEIFGYQLNTWQMKESAGKAT
                     RSLSVGEYNYLLLLAGKHPEYVLCKRDTITAEPTTV"
                     /name="hypothetical protein"
     gene            complement(17064..17321)
                     /locus_tag="QDW64_RS23650"
                     /old_locus_tag="QDW64_23645"
     CDS             complement(17064..17321)
                     /locus_tag="QDW64_RS23650"
                     /old_locus_tag="QDW64_23645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000845367.1"
                     /translation="MKTDNVFFKSKGSYSYVVTVDMDNQMYMVRKYVKTYVKSCDKEQV
                     KGKKTVKKLVLDTNLTLPELLDLDNGILADLFTRGNNNAN"
                     /name="hypothetical protein"
     gene            complement(17357..17899)
                     /locus_tag="QDW64_RS23655"
                     /old_locus_tag="QDW64_23650"
     CDS             complement(17357..17899)
                     /locus_tag="QDW64_RS23655"
                     /old_locus_tag="QDW64_23650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000206647.1"
                     /translation="MTTFSEKYQPVFEVVCRILGNGWRVNKLDNYQHRIKLTSTEIRKY
                     SIIIRLERERFFIVGSVDSLVWHGNPSVCTVSLTRNPVGIANDISRKILANALDEIELL
                     REHESALQEKQDKVRIIKGMLSQLLSISPWYGALTGFNADNGLAGKILQNGDDYEMVIR
                     GLNVDQLIKVAGFIKKL"
                     /name="hypothetical protein"
     gene            complement(18027..18560)
                     /locus_tag="QDW64_RS23660"
                     /old_locus_tag="QDW64_23655"
     CDS             complement(18027..18560)
                     /locus_tag="QDW64_RS23660"
                     /old_locus_tag="QDW64_23655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="WP_001025389.1"
                     /translation="MNIQEALNIFGLSGQLTEKDIKNAFKKAALKYHPDRNPVGAEMMK
                     AVNSAFDFLMANLDKINQYQSPEQSAIYNYGDELENVLKALYGLSGLVYEVIGNWIWIS
                     GNTIEHKEALKEIGCKWAAKKKQWFYRPEEHKSYWNREEHTIEEIRAKYGTSGKFQAGG
                     WQQVEGRARVEARA"
                     /name="J domain-containing protein"
     gene            complement(18931..19230)
                     /locus_tag="QDW64_RS23665"
                     /old_locus_tag="QDW64_23660"
     CDS             complement(18931..19230)
                     /locus_tag="QDW64_RS23665"
                     /old_locus_tag="QDW64_23660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000113288.1"
                     /translation="MARLPYIESTTQQIFIFNTLTLCTIGLIHIETGEKYIVVFTDNNK
                     IRDYKNGVIEELGEITQEDIELIEYYAKKYEKYFDDLKIGEEPLNLKEYIECMQ"
                     /name="hypothetical protein"
     gene            complement(19304..19576)
                     /locus_tag="QDW64_RS23670"
                     /old_locus_tag="QDW64_23665"
     CDS             complement(19304..19576)
                     /locus_tag="QDW64_RS23670"
                     /old_locus_tag="QDW64_23665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001272119.1"
                     /translation="MSAFIFRTTFKATGDQQKDAITQIEVMQEQAVKAFVSWMTNQEGT
                     EFLSTLQEITTELDALIDAVESHSPVFRKKATELEALLAHYKTLL"
                     /name="hypothetical protein"
     gene            complement(20527..21192)
                     /locus_tag="QDW64_RS23675"
                     /old_locus_tag="QDW64_23670"
     CDS             complement(20527..21192)
                     /locus_tag="QDW64_RS23675"
                     /old_locus_tag="QDW64_23670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371526.1"
                     /GO_process="GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA family replication protein"
                     /protein_id="WP_001350943.1"
                     /translation="MAKQSPCYNVVSRKRRRFLNRWIKSLTTVAGRIKIKNELRRRIRH
                     NRYWVNEANKFGIEALCELMLAILDDLDLRSWETRHNLETLAERAGLVTRSDAGHKSIS
                     RASRGCDRLVWLNAIITEKAPFNPYDARCACKHIEVTEDFFAILGIPLKLVYRERARLL
                     KADPDEVIYSGDARLIAIRVQNWTRKAAAGLARMKAKRDAARQRKKEYYAHSPTPIPA"
                     /name="RepA family replication protein"
     gene            complement(21808..22173)
                     /locus_tag="QDW64_RS23680"
                     /old_locus_tag="QDW64_23675"
     CDS             complement(21808..22173)
                     /locus_tag="QDW64_RS23680"
                     /old_locus_tag="QDW64_23675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248966.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000248966.1"
                     /translation="MVPKRLKEARMEAGLSQEKLSQLLGVAEGINTRSRFSSYEVGRTE
                     PPFKLVKKIAELLDYPENYFYTEDDDFAKAVLDFHRKRTSPELNPNYDSEVEARRMASK
                     LDNVKKIVAQLNEYLKE"
                     /name="helix-turn-helix transcriptional regulator"
     gene            22428..22877
                     /locus_tag="QDW64_RS23685"
                     /old_locus_tag="QDW64_23680"
     CDS             22428..22877
                     /locus_tag="QDW64_RS23685"
                     /old_locus_tag="QDW64_23680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011998906.1"
                     /GO_process="GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]; GO:0006355 - regulation of
                     DNA-templated transcription [Evidence IEA]; GO:0140673 -
                     transcription elongation-coupled chromatin remodeling
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="WP_000995159.1"
                     /translation="MNAWYLLYCNNNDLDRIAYRCKTMNVVAFCPRYIKITPRKDCNAV
                     RKEEKVLFPNYLFLHFDVNVTHTSAITSIPGAIGFVSFGSTICTVPEKVVIAIKCAKIL
                     ALNPDDDSIECRNIGSDLLAEIQRISSIISPLERQTAFSRLLQNQ"
                     /name="transcription termination/antitermination NusG
                     family protein"
     gene            22954..23166
                     /locus_tag="QDW64_RS23690"
                     /old_locus_tag="QDW64_23685"
     CDS             22954..23166
                     /locus_tag="QDW64_RS23690"
                     /old_locus_tag="QDW64_23685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001133545.1"
                     /translation="MPNPIGQAKSTAVIYIGKKRYETLAKHARDISYVAEVNIRPSAFL
                     QYMIDKLSNEAHNEMLKELMSKEDK"
                     /name="hypothetical protein"
     gene            23167..23373
                     /locus_tag="QDW64_RS23695"
                     /old_locus_tag="QDW64_23690"
     CDS             23167..23373
                     /locus_tag="QDW64_RS23695"
                     /old_locus_tag="QDW64_23690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125884.1"
                     /translation="MPKPTYNVYVTQEVPTDENGGKNTYWTKVGAAFAHNGKPGLNIML
                     VPGIAVSGKLVLLEPKEDAPQGN"
                     /name="hypothetical protein"
     gene            23389..24093
                     /locus_tag="QDW64_RS23700"
                     /old_locus_tag="QDW64_23695"
     CDS             23389..24093
                     /locus_tag="QDW64_RS23700"
                     /old_locus_tag="QDW64_23695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing protein"
                     /protein_id="WP_000953548.1"
                     /translation="MLSTVSFMAVAMQCAATVHPSTSLDVARVESGFNPYAIAEIIPKR
                     ERQPGDKGFISHMPKTKEDALSIVKQIEAKGRRYSVGLMQITSTNFNSYAVTAADLFNP
                     CTNLSVFEKIITDCYQRGGTLKRALSCYYSGNFTTGQQPEAALSRTSYIQRIGYSPEKP
                     RYVVPGTRDDIATQSAILNATPVEAPTRPRVVWPGAIVRGVPAQLRQKKADTVYYPAQV
                     IRGNRFSTEKED"
                     /name="lytic transglycosylase domain-containing protein"
     gene            24097..24393
                     /locus_tag="QDW64_RS23705"
                     /old_locus_tag="QDW64_23700"
     CDS             24097..24393
                     /locus_tag="QDW64_RS23705"
                     /old_locus_tag="QDW64_23700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012885319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="WP_000608756.1"
                     /translation="MINKLKAICSRASAYILAPLVSVVSPVFAADGFSRANTIMEKVSN
                     GLHGLAAVTITVAVIWIGYKTLWKGESLSQCGYIIIGGILIGGGSEIGALLMS"
                     /name="TrbC/VirB2 family protein"
     Typing          12876..13606
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.361"
                     /coverage="100.0"
                     /evalue="9.03e-162"
     Typing          2..2742
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="87.527"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4970..5649
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="85.463"
                     /coverage="100.0"
                     /evalue="4.2000000000000004e-119"
ORIGIN
        1 atggcaaagc tgcttaaggc gatgaaacgt ccggcggcgc tgtggggggt gccaatggtg
       61 cccctgctga ctgtcgtggg cgtgacaatt attgttgcta tctggacttc catagcccta
      121 cttctcttcc tccctattga gtttctggtt atgaaatcac taaccagaaa tgaacctctg
      181 agatttaacc tgatagctgt ctggctaagg gtaaggggta atccggtagc aaaccgttta
      241 tttggcgcaa ccgcatttat gcctgttgaa cataatgctg ttgatatcca ggagtttatc
      301 gatgcgatga agctaaacca atgtgccacc ataaaaaaat acattcctta ctcgtcacat
      361 attcacccta atgttgtccg ttcactgaat agcgattttt tctgtacctg ggaactgaca
      421 ggaacgccgt ttgattgtga atctgacgag acactacagt tcggtacaaa ccagttgcac
      481 gggctaatac gctcatttga aggctttccg gttacatttt atgtacataa cagccgtgaa
      541 cagtttacgg actcactgca taaggtttca ggaaaccctt acgcggatac cgtgagcgaa
      601 cgctattacg catcactgaa taaaaggcca ttccgacgta accgcctgtt tgtgaccatc
      661 tgctatcgtc cgtttgccag tctggataag gcaaaccgta aacgtatgaa agacggtcag
      721 aagctaaaag agatagacag cgcattactt gaagtgctgg aaatcaaaag tacgctggat
      781 acagccttat cccgttatgg tgctcacgcg ctcggtactt tcaccgaaaa cgaggctgta
      841 ttctcgtccc aactggcatt ttatgaatac cttctcacgc atcaatggcg aaaagtcaga
      901 gttacacgta cccccgctta caacgtaatg ggaaatgttg cgctgtactt ctccgccgaa
      961 tcagggcaaa taaatcatgt taatggcact caatatttcc gtggtctgga ggttaaagag
     1021 ttttcggaag aaaccgcaac gggcatgaca gactccttac tgtacgcgcc gtgcgattat
     1081 gtgattacac aatcctatac ctgcatgtcc cgtgaggaag cgaaaaaggc gattaagcgc
     1141 acccgacggc tgatgatgag tgctgatgat gatgctgtat ctcaacgttt ggatctcgat
     1201 gtggcgcttg acctgctcac ctccggtaaa atcgcctacg gtaaacatca tttatcaatt
     1261 atggtgtttt cgccgtcact ggaaagtctg attgcagaca caaacgagat cagcaatgca
     1321 ctcaacaata tagggataac ccctgttcct gctgaaatct cattgtctgc ggcctatatg
     1381 gctcaattac caggcaatta caatctgcgg ccacgagcgg gagagttgag tagcctgaac
     1441 tttgttgaac tggcatcact tcataatttc tatccaggaa aacgcgacaa agccccgtgg
     1501 ggtgacgcga tggctctggt gcgaaccccg tcaggtgatg gctactatct gaatctgcat
     1561 aacactctgg cagataaaga cgagttcaac gaaaaaaatc ctgcaagtac ctgcattctg
     1621 ggtacaaacg gctcaggtaa aacaatgctg atgacgtttt tacaaatcaa tcagcaaaaa
     1681 tataaccgtg atgacagctt ttcaccggat gccaggacta aacgactaac cacagtttac
     1741 ctcgataaag accgtggtgc tgaaatcaac attcgcgcac ttggaggaaa gtactacagg
     1801 gtattcagcg gtgagccaac aggctggaat ccgtttgctc tgccagccac taagcgaaac
     1861 atcaatttca ttaagcaact gatgaaaatc ctttgtaccc gcaacggtgc aaccataacg
     1921 ccacgtgatg aacgtcgcct caatgatgct gtaaatgccg tcatgagtga cgagcctcaa
     1981 tatcgcgtct acggcataac ccgaatgctg gagaacctac cggaaccggc aacgcgtgaa
     2041 gcgcaagaaa atggcctgag tatccgtctt tcacagtggg cgcgcggtgg tgaatttggt
     2101 tgggtgtttg ataacgaaca cgacactttc gatatcagca actgcgataa tttcggaatt
     2161 gacgggactg aatttctgga tgacccgaat gtctgcgccc ctatctcgtt ttatctgctg
     2221 taccggataa ccagcctgct ggatggcaga cgacttgtta ttttcatgga tgaattctgg
     2281 aagtggttac gcgaccctgt atttaaggat tttgcgtata acaagctgaa aactatccga
     2341 aaactgaacg gtatgctggt tgttggcacg caatctccag cggaaataat caaggatgag
     2401 atagctccgg cagttattga gcaatgtggt actcaaatac tggccgccaa cccaagcgca
     2461 gatcgttctc attacgtgga tggaatgaaa tttgaaccag aagtgtttga tgtcattaaa
     2521 ggacttgatc cacaggcgcg acagtatgtc gttgtcaaaa atcagttccg ccgtggagat
     2581 acaaaacgct ttgcagcccg tatcacgctg gatctgtctg gtatcggcag atacacaaaa
     2641 gtaatgagcg gcgacgcacc taacctggaa atattcgaat cactctatca ggagggaatg
     2701 aagcccagcg actggctgga ttcctaccta gctaaagcac tataaccaag gagatatcga
     2761 tgaaaagatt tcgccccctt attctggcgg ttcttattgc cagcccgata gtaaacgctg
     2821 ctggtatacc cgtgtttgat gcaacagcca gactagagaa tattcgccag tggacaaaag
     2881 aagccaatca atggctacag acggtacaac actataaaga ccagctacag gcgtatcaaa
     2941 aacaactgga aacagttaca ggcatccgtg acattcaggc gttcgttaat caggccaaaa
     3001 gccttaaagc tgacctggat aaactacgta agccagggca agccctgaac gatttgctct
     3061 tatccggcgg ttcttctggc caatttgatg aactgtacaa caaatacaag attttcgata
     3121 actgcaatgc tgaacaatca aaaagttatg ccaatacatg cagacaagag gtcatcaaca
     3181 aagcaataca aatcgaacaa accaatgaag tacaaaccca gattggtgag atgcttggtg
     3241 agatcaacga attatcagac cgtgtagcaa atgccaaaga tatgaaagag gcccaagact
     3301 tagcaaacag tattcagtta aaatctgtga tgctaaatac gctcacgacc caatgggaaa
     3361 tgaacgtaaa agcggcggag aagcgtgaaa agatgcttgc tgatgaacgc cgtaagaaat
     3421 ggaatcagca gcaattaaac gcaccagaaa taatgtttaa ttagcaggtg acttaatgaa
     3481 gaaaatcatt ttatttacaa ttatttcagg cctgatggcg ttaacttcag gatgtaagga
     3541 agaagctaaa acaacaaaat ggtatcgtga acatcctgat gaactcaaag ttgtttatgc
     3601 tgaatgccag aaaacaggta gcgcatctga aaactgcaaa catgccaatg aagctcattg
     3661 gcaaattcaa caattaaatg cacctgaaat aagtttcaac taaagggcgt ttatcatggc
     3721 cggtatattt tcttctgtcg aaaaaaatat catcggtggg ataactgacg cgactaatgg
     3781 tttgatgatg tcttattcgt caatgatgat ggggctggcg gctacgtcag ccactatcta
     3841 cattatgtgg cgaggatatc aaaccttagc gggaaagctg tctacaccaa tggaagatgt
     3901 tatgtgggat ataatgagga tggctatcat tttatccttt gtagcaaaca ttaacggata
     3961 tcttgatggt gtcattgatg ctgtaaatgg aataaaagaa ggtttttcca gtagcacaag
     4021 catctggcaa ttacttgata ctctatggaa cagcgcaaga gttttaggta aaacgttaca
     4081 tgatctagac aactccgaat ttatcaaaga ggaaggcaca attgctgaat tatttgtatg
     4141 gggtggtgtc tttgtcttaa tgattatcac agcattcgta tctatgattg cggagatcat
     4201 gatcttattg ttaggtatta ccgcaccaat atttatattt tgcctgatgt atggcttttt
     4261 acggccaatg tttaataact ggcttcaaaa tatatttgct gcaatactaa caattatgtt
     4321 ttcagcctta gctctgcgaa ttgtaattgg ttttcttaca tccagaatgt cagaagcagt
     4381 gacccaagcc agcaaagcaa acattgtaga acttggcgct caagtttgtc ttgccggagt
     4441 atgcgctgca atactggtgt acatgtccgc aaaactagct ggggcacttg caggagcaag
     4501 tgctacagcc actatgcaag gtgcggcggc tctcggcatt ggcgctgctg catacggcgc
     4561 tggaaagatg ttaaaggagg caggaaaagt attacctaaa gaagtagcaa gcccaagtaa
     4621 taaagggtta ggagatccac aatcgcgcat agtatttaac gctgctgatg cacgtaaaca
     4681 cgctatagaa caaatgatag cgcgtaatga agcgcggatg ctgacagatc aatcagcacc
     4741 aaaactaccg ccatcaatta aacgtttacc tcctccaaga taactataaa aatataatta
     4801 tatttttata gaaatacaaa aaggtacata aaatgaaacg cttaatcgcg ctggctttgc
     4861 tgtgcgtgct ttctggctgc gcacaaaaat ctaaatctct gccgcacgca ttcagcggtc
     4921 agggtagccc tgtaaacaca ccccaaatta tcgcggagct aaccacacat gactgaacag
     4981 aaattaattg ctgattcccg ctcgtttgag cagcaaatga ttgacaggga taaacgcgcc
     5041 acaaaagcag gctttctggt cggtggagca gggttattaa tcactatcct gtcactgatc
     5101 gcgatagtga ttatgttgcc acttaaacaa acaacagttg aactctatac cgtagataat
     5161 cataccggac gagtggaaca cgttacccgt gcatcaaaaa cagccctgac agcggaagaa
     5221 gcctaccaaa aagctatggc cgccaattac gttaaattac gtgaacggta cgtttatcct
     5281 gcattacagg acgattacga gacagtacag atctacaact caccacaggt aaacgacgac
     5341 tacctggcac tgtacgccgg aaaaaacgca ccagacaaag tttataataa cgcggctcac
     5401 accgtgaaaa ttgaaattat ttctgagcag ataacgaatg caacagcacc agacaaagta
     5461 gccaccgttc gttataaaaa aataattcgt cgcatggctg ataacagtat tcgcaatgaa
     5521 tactgggatg cacgtttcac cttccattca gaccctgaaa aagaaatgtc agatgttgag
     5581 cgggaaataa acccgttcgg attcaccgta acaagctggc agactgaccg cgaaatcagg
     5641 ggaggcgagt aatgagaaaa ctgacgttaa ttaccctgat aataatgctg aacagccagg
     5701 catgggccgc agctacacca caagcgagcc gttatgatgc acgtatccag caggtcattt
     5761 ataacccgca gaatgttacc gttgtaaaca caaaaccagg atttatgacg actctggttt
     5821 ttgataacga tgaagctgtt atcagtgcca gtcctggctt tgatgaagca tgggaagcca
     5881 gagcagatgc aaacagggta aacattcgtc cggttgcgct cacacagggc gcacctggtg
     5941 aagacggtaa tacaacacag gttgttatcc cgccgaacag ccgtgactgg cataccaata
     6001 tgctgattgt gacaaataag cgcctgtaca acgttgagct gaatgtcatt gatgaaaaat
     6061 cgccacaaca gcccgcatac ctggtgagtt accgctatcc aggagacgaa cgtaacagag
     6121 ctaataagga agcagcagcg cgtcagaaag aatgggaaca aaagcaggaa caaacccaga
     6181 ttcagaaagc actgagaacc gcacaaaccc cgcgtaactg ggactacacg aaatatccag
     6241 gaaaagacag ttacaacatt gaacctgatt ttgcctacga cgacggtcgc tttacgtttc
     6301 tcggattctc acccaacaaa tcagttccat cagttaccaa agaacagaac ggccaggaat
     6361 acgtggttaa cagcagtata cagcgtaagg gcaattttac cgttctcgtt atccaggaaa
     6421 tcacaccacg gcttgtatta cgttccggtg atgccgtagt ggggctgaaa aataacggct
     6481 ttggaaaagt acacgcggct gataattcaa ccgtatctga caaggtggag cgcgttgaaa
     6541 aaacagataa cacctaatct ataaaaatat aattatattt ttatataagg actcatatat
     6601 gactgacgaa aataaatccc cctctacgga agaagaaaag ccagtgatga catctgatca
     6661 ggatgatatc gccacgctgg aacgagaagc cagagcaaaa cgtgaggctg aattacaagc
     6721 atcacaggac gatgaaaagg agaactcatt acaaccagca gtaaacaaac tgaaaaaacg
     6781 tcagaagggg aaagcagcgg cctttctggc aatatgcgca gtagccctga ttggcctgac
     6841 atggggcggt aactgggtat atcaaaactt cctgaagcaa gaaaatggag agaaaacaaa
     6901 ggaggttaca ccacctaaaa aaacgaatga ctaccggaaa cgaaccgatt taggcatggc
     6961 caccaatgag ccggaaaatt cgactgaggg tgagcaagct gttagccagg ataacacggg
     7021 caatgtatcc ggtactgccg gacagatgaa aacatttgag ccaccacaac tgaataaggc
     7081 cagcttcctt gttcgtcgta ataatacggc cagcccccaa aatcaggtaa aaacaagaca
     7141 acaggaaatg atactggctg cggaaaacaa cgccacacag cctacagaca gtacagcaac
     7201 cacaacacaa catggcactg catcacaggc aatttccccc gcaacagtgc gaagaatccc
     7261 ctacaaccct gatttgtaca taccggaaaa cacagtcatt ccatgctcta tggattaccg
     7321 ctttgtttcc gatcgcgctg gaaaaatacg atgtacggta gctaaagata tctggagtgc
     7381 cagcggcaat acaaaactta tcgagaaggg aaccacagca acaggaatat accagacagg
     7441 aattacgcaa gggcaggggc gcgcttttat catgatgaca aaattacgca cccgacagcg
     7501 accatatctg gatattccgc tgattgatac caacgccgct ggcgagcttg gagaatcagg
     7561 tgttgatgga tggattgata cccacttctg ggagaggttt ggcggcgcat taatggtcgg
     7621 catgatccca gatattggcg catgggcatc aaacaatgcg ggtaaaaaag accgcaatac
     7681 agactataca gagaacagcc gccaggctat ggcggatatg gctcgaacca cgctggaaaa
     7741 cagtatcaat atccccccta cgctctacaa aaaccagggc gaaattatca acctgattac
     7801 agggcaggat attgactttt caaatatcta cacgctgagg ctgaaaaatg aactgcaaag
     7861 ataaagagac ttggttgcac atgctcgccg ccggacacat ggggggtctc gccgggaacc
     7921 cgaacgaagc cgccgcacag gatgcttacc agcgtctgat taaaaacaaa accgcacagg
     7981 ccgttttgca gcggtgtacc accagaatca cactgaaagg agcaaacgat gaccgctaag
     8041 aatatttccc tggaccgcta taaacagcga ttttttggtg actttctgga attaccagga
     8101 ctgacagaaa tagctgttaa tcgtccaggt gaactctgga caaagataga tggtgtctgg
     8161 gggcaacata ccgttgatct gaaatttgac gattgctata actttgctcg gtgtcttgca
     8221 aaacaccata acgataatat cgaggatatc aaaccaggac tttctgcaac cctggaatca
     8281 ggagaacgat gtcaggttgt cgtacctccg gcgtgcgaac gtgacacgat ctcaatcacg
     8341 atccgtaaac cgtcaaaaat tcaaattccg catcaggcat atattgatag cggtttttat
     8401 aaccgcgtca caggcattga aaaagcatcc acacataacg aagaattgat tgaattatac
     8461 aaatcaaaaa acattccttt gtttatggaa aaatgtgtgg agtatggaaa aacattagcc
     8521 gtcgcaggtg aaaccagtac gggtaaaaca acatacatga aaatgttgat acaatatata
     8581 cccgtccatt tacgcataac gacgatagaa gacaacccag agataatatt ctacaaacat
     8641 aagaactatg ttcatctgtt ttatccttct gagtcatccg atgaaaaagg ttctgttgta
     8701 acaccagcgg cgcttatacg ctggaattac cggatgaatc cagacagaat tttattaaca
     8761 gaagtgaggg gtggagaggc atgggatttt ctgaaaataa cggggtcagg ccatgaagga
     8821 agtatgacct ccattcatgc tggttcagcc catgaagcaa tcgacggttt tattacccgc
     8881 tgtactgaaa acccacagtg tgcgcaatta ccatattcat tcttgctacg taaagttctc
     8941 gactctctgg atgttgttgt gagcattgat ttagatggaa acgtacgccg tatgaatgat
     9001 atttatttcc gaccgttaca ccgcaatcaa tattacgagg aaatgaaagt atgataaagc
     9061 atatactggt tactgtgctt gtaacactaa ttaccggatg ttcctcaccg cctccaccag
     9121 tacccgtgga atggaataaa aagccggtat cgcttaacga gaaacttcct gaatggaacc
     9181 ccaatagctt aataataaaa tcgcctgtta ttaatgacaa atggacgtta tccattagct
     9241 cacaagattt caataaagaa gattggatgc catctgtctt ttatgccatt gctcactcaa
     9301 cacaaatcac agtagcaaca aagtcaggcg ctgattattt cagtgcaaaa aactggttgt
     9361 atcatcatgg tgcaaacggg gtaattatat atcaccccat cacaaattgt cttacgtgca
     9421 atgaaacaac catctacttt tcgcgttaac acaaaatatt aaacaaaggg ttctgacatg
     9481 aaaaaaattg cagcaataac aatgtttgtt ttaacaaccg ccacacctgt ttactcttat
     9541 gccgcagacc catgcgaggt tgttttatgt atgtacggaa aagctacagg aaacagcgga
     9601 ggtggcgaat gcagatcagc agaaaaagca ttcttcaaca ttcttaagaa aaagaaaggt
     9661 tcaatacgat ggagtaaaac atttgatgca cgtaaagcat ttttaaatca gtgttcatcc
     9721 gctgatcccg cagctatatc aaaaataatg agtaagtttg gtagagttcg agggtaaata
     9781 tgaatacaga tgaaaacatg gtattttatc tgacaaactc cgatcttgtt tatagctatg
     9841 cacacaagat agtgtgctcc attccatcag gaatgaaagg gaatttcatt acttctatcg
     9901 gcgaaacgct tgaagattta aagaaacgat atcctgatat tattctcatt acagaatcgg
     9961 aagcattagc attgcaatcg aagagggaaa attaacatat tctttcagat ctggagaatc
    10021 ctatgttaaa agaagatatg ttcattaata atattcattc aaggaatcag gacagaatcc
    10081 atgtaaaaag agtctttgat gatattagta ataaagcaaa caggggatgc gggctatatt
    10141 atgaaatata tcaagcgagg ttaatgcgag ggttaattga gcacacagta aaactgaaaa
    10201 gttctgatgc caataagctg attagttacg ctgcttcaca aggttatgaa ataacagaag
    10261 acagatataa tgctgcaata aaagccgagg atgaatgtct gactgaaata acacgggcac
    10321 aggtctgagg cagaggattg cgtagcaacc cgaagggcgg ggcaaattcg ctttgcgaat
    10381 ttgggcgagt ccgacgcagg gcgtggtgca gttcaggctt tctttatttc tggcttcaat
    10441 ttcgaagaaa ttgttaagac aggaataaag aaagtggtgc gtggacagaa aattcaattt
    10501 cgaagaaatt gttaattttc aggcaccacg cccacccgcg aacattcgcc ccaagcgaat
    10561 tgaagcgggc cggggggagc cgcaaaaatt tttccgcccc agcgagaaaa aaggcacgcc
    10621 cacgtgccgg tttttgtggg gggcttgccc cgcacaccgt cgggccaggt aatatttccc
    10681 gccccagcgg gggatattac cgattaggcg cgaccacccc ctttaaagca gcgttccaat
    10741 tttttcgagc ttgcgaagaa aaaataggct aaacgcgcgt cttaaagggg gtggtcgcgc
    10801 gtagcgcgcg acggtgtgcc gcctttgacc ttgggggttt tggtccggcg tccagacggg
    10861 ccattacccc catgtgacgt gaaggactgg cgtttcagcc aggccgaaac ggcacacgcc
    10921 gttgcccgtt cttcgggctt tcaggagcca gacgataact tatcggctgg cttctgaaag
    10981 aacggataac gggcatgatc gccttaggcg atcacaacgt ttttggcttt tattggggta
    11041 caataatggg gtacagctat ggggtacatt atatccccca ttactgtacc ccattgtcgt
    11101 accccataga ttcactttat gaatggggaa aacaaatatg aatccacatc taatgcaaaa
    11161 gctgaatggt attgctgatg ccttttcaat atttctgtta tggctacaga acagccctgt
    11221 catattgtca ttactggcag gactaacact accatttata ttcaacctac cacgagagga
    11281 aagaaaaaac gctcccttct ggttaaaatc tgttgcgtgc gtgtccattt tctttttcat
    11341 attcggcaca atgtcaccac ttagtattca tggtctgagc tacctatttg acgttctcga
    11401 tcccaataca ctacttagaa taccattatg gataatcaca attctcttta cactagctgg
    11461 attagctttt catttgaccg cgcgacgatt attagcaggt gaaatagaca gcctgaaaca
    11521 cagaatgata aagaaaagca aacttgagag aaatacaaga acagatgttc gcaaagtaaa
    11581 agaattgttg ccagacagca tagaatacaa tcccctagat tacattgatt tgaaaaaagg
    11641 ggttttcatt ggcttagaca aagacgacca accgcaatat ataacaataa aagaatttca
    11701 gaaacaacat gcagcaatca taggtacaac aggttccggt aagggcatta ccgcatcggt
    11761 tttgctttat caggcaattc tgttaggcga ggctgttttt gttgaagatc caaaagatga
    11821 tgaatgggct gtgcatgttc ttagggaagc atgtaaaaaa gcaggaaaga aatttgttgt
    11881 aattaattta aacagaacca accatcaact tgacttactt gctgacatat cacatgaaca
    11941 attagaagaa ttatttaatg caggatttag tctatcaaaa aaaggagagg ccgcagattt
    12001 ttatcgtata gctgatcgcc gcgcagccag aaatgtagcc gcaattcata aaaccggaat
    12061 gacactgtat gatttattca acgatgacaa cgtaaaatca ataggagaag atgccccagc
    12121 atttttaggt gaactagaag aaattgctct tgtaaattcc gtcaatgcaa aaaatggatt
    12181 ttctcttaaa gagatttttg ataatggagg atgctgctat atcattgggt caacccgaaa
    12241 tcaaaaaatt atttctgcac aacgcatgat acttacgcgt ttgattcaga tagctgaaac
    12301 cagagacaga ataaacacaa ctccacgtgt tgttgcaatt tttcttgatg agttgaaata
    12361 tcatatttca cgcccgacaa tggaaggatt aggtacagca cgagataaaa gtgtgcatat
    12421 atttatggcc ttccaggcaa tagatgattt aagagattgc ccttctgatc ttaatggcga
    12481 ttctgtaatt ggtgctgtta ttgaaaacgc aaaattcaag ttaatctata aaatccagaa
    12541 tccagaaaca gcagagtggg cagctaaaat gactggttca atattagttg acgatgaaat
    12601 gaggaaggtg agaaccgact tatcattaac agaaaaagtt gatactgacc gcatgataag
    12661 acaggcagaa agttattatg tagacagtaa tatgttttta aatctacctg aaagagtagg
    12721 atttgtgttc acatcaaaag atttaccaaa agcaactaaa atgtctcata taccagcaaa
    12781 caaagaaacc attgaattgc tatcttttga gacagaacaa atagaacaac acaataacaa
    12841 cgatgtgcct tccagtggtt tatgaggttt tactatgtta attcatgatt tttacgaaag
    12901 aaaaagaata tcagctaaca acatgctttg tcttttaaat ttccttaagg atgaaacgta
    12961 ttcagacttt cacattataa aaagaaaatt aggattcaca aaaaaaagca gaaacccagt
    13021 atataaatta ctaaacaagg ctcttaaaga aggttttatt atcaaacatg aaataccatc
    13081 atcaaaaaga aaattaactg tttggggtat tactatgcaa ggattagcaa tggttgtaaa
    13141 agccgatgac attacgttcc ctagttattt cgaacccagc aaactaaaat actggactct
    13201 tgagcatcgt cttttaaatc agcgagtgag aatatctctg gaaagtaaag gcgctactga
    13261 atggattaac ggtgatagtg cagccttcat cgcacgttat tccggctgtt cccatcgccc
    13321 tgatggagta ataaaacaac caactggcgc aataatggct attgaaacag aacgaacaat
    13381 gaaaactcgt gctcgctata taagcattat aaacagtcat cttacggcaa gagataaagg
    13441 aaactggcat tatgctttgt acgtcatgcc tgacaccaaa agtaaagaat cattaatcag
    13501 actgttcgac tcaatcaaaa ctgtaatgag gaataataca cctgtgcctt ttgatgcaaa
    13561 aaacagagag atgtttattt ttagaacaat tgatgaactg gaacaatagt tgatgaaaat
    13621 attaatcata ttcgcattat atatcttgtc gccttcacta tatgccaatg cagcggatat
    13681 acagggaaaa gttattcgtg tacttgacgg cgataccatc gaaattttac agaataaaac
    13741 gccagtaagg attcgtctgg ccaatattga tgcaccggaa aagaaacagg cttacggacg
    13801 ctggtcaact gaccaactta aaagcctgat tgccggacaa tccgtcaggg tgacatattc
    13861 acacaacgac cgttacggtc gcattatcgg acgtgtattc acaacaggcg gtagtgaggc
    13921 taaccgcttt atggtgcaat cgggtgctgc atgggtgtat gagcattaca acacagataa
    13981 cgctctggca gatctacaac aggaagctcg ctcacaaaaa cgaggtttgt gggctgaccc
    14041 caatcccgtt ccaccgtggg aatggcgaca taaataggag attgacatgc aaacaattac
    14101 cacacgtgaa cagttattag tgaatgggaa agtcctggaa agggttgcga cacatatcgt
    14161 aactggcgct catgggtatg aaacgctttg tacaggtggc tataacttac agtacaacca
    14221 agagcgagta ctggttcaaa attgtgaaaa gctggctgaa ggcgaactgc ccatcacctg
    14281 ccacacttgt ttcgctgtat ggcaggatgc atacaaattc aaagatagcg actttgacat
    14341 aaaatcagga aatttcactg atacaggtct gacagatatc acaattggcc agatttcaga
    14401 accagaagaa gaaagtgtga atattctcta tgcggatcaa tacgaaacat tgccaataga
    14461 acgctatatg acaaatgaag aagtaggcag attagtgttc aattttatga aacgcaccaa
    14521 cggcgaagcg ttactaatag tgtttccaga atatgataat ggagacgata atgacgataa
    14581 aaatactcgt tgtgtcaaat gacggccatg aaaaactaat tgatctaagt ccagataatg
    14641 atttagctaa aattacaaaa tcattgcgta cagcagaaaa caggatggta tgtgttatcc
    14701 aggacaataa tcgtattctc cgctgggaca gaagctatgc ctctcgtgcc aaaaatcact
    14761 ggcgaaaggt agctcccgat cgttttgaga ttttaggaac cgttgaacat attcattatg
    14821 ttggtaagtg ttaatatcag gggacagttc gtcccctatg ccattttaat gatgcttttt
    14881 ccagaattcc tggtatgcct ccagcaactc cccgtgcttc atatcattag ccgctgcaat
    14941 cgagcggaac tcccgtttaa cttccatatc cacataaagg ttaagctgag cttttctccc
    15001 atcagcgttt ttacgcgtcg gattaaatcc gtgttgtgta tcctccagac ttggcggctc
    15061 gcctttggtg ttacgcggtt ttggtggtac attagccatc ttatcacctc tataaaaata
    15121 taattataga attatatatc actgtgagag tttagtcagc aagtcaataa ctccctggac
    15181 aacttcgtca gctttctgcc gtggacctgg aaaaggggat tccgtcaatg ccagtccttt
    15241 atcctgtgcc tggctgaacg aggtcataaa aggtattgcg ccattgataa cttgaacacc
    15301 acacggttta agatattcaa gcgcggtacg atactcactt tcgctttcac tgactccgac
    15361 aaaaactaca gccatcttat ttgttgagtg tcccttgtta cgcaatgcat tcacggttgt
    15421 aaccgtcgtt ctcaaatcgt ccacactaaa accagacggt aacacgagca aatcaacaat
    15481 ttctgccagg ctcaacagcg atttagttgc ataagcagca ctatcgataa cgactaaatc
    15541 ccatatccct gattcaatat gttttttagc ctgtgatacc gttccgcatc cctgaatagt
    15601 gacaggtggt tcaactttat atctcaatcg ccgctgtccc caatccagga atgacccctg
    15661 atcaacatcc atatcaagcc ctaatacctt ccagtctgcc ttcgtgtact ctgtggcaat
    15721 agccctgctc attgaggact ttttcacccc gcctttaccg ttaaccagcg caatagctac
    15781 cgtcatacga ccccctataa aaatattttt atagaaaaat ataattatag tatccgtatt
    15841 ggtgccaaga attgcaacaa ctatttgaac tacaggcggc aagtgaagag ataacttgtg
    15901 aatataaggc tttcacaacc aaattcagcc aaaacaaaaa atgacgtttt attaacaaat
    15961 acgcaacata aaaaacggtg gaaaacgccc aggaaaacat gatcttttga gcggattttg
    16021 cagatccgac aacgaacggt gatccgatgg tgccgataac gtccattatg ttaaatagcc
    16081 ccgtttccct tgaaattata agtcgtagct tatacaatca tctataagct ataacttata
    16141 ggaaaaggga catgtggact gtttattttg gcagattatt tgatgaatgg tttgaagagc
    16201 aggaactagc cctaaaacga aaagtgctgg ctgaacttaa acatcttgaa gagtttggcc
    16261 cctctctttc acggccacat gcagatacag tgaaagggtc acgtcataag aatatgaagg
    16321 aactacgtat acaatatgag ggacacccaa tcagggcatt ctttgcattt gatccaatta
    16381 gacaagcaat cattctttgc gctggcgaca aaagtaatga taagaaattt tacgacagaa
    16441 tgattcgtat tgctgacgaa gaattttcca ctcacttagc cgaactggag ggcaaaaaat
    16501 gagaacatta gaagaagtaa ttgcggctca atcacctgaa agtcaggcga tcatcaaagc
    16561 tatggctgat gagatgattc tggaaacagg gctgcaaatg atgcgtgaag aattgcagct
    16621 ttcacaaaaa caggttgctg atgcaatggg aatcagtcag cccgctgtaa ctcaactgga
    16681 acagcgcgga aatgatttga agctggcaac gttaaagcgt tacgttgaag ctatgggagg
    16741 aaagttgagc ctagatgttg aactccctac cggaaagcga gtggccttta acatttagta
    16801 gaaaaggggc gaaaagcccc ttttggggtc agacagtggt tggttctgcg gtgatcgtat
    16861 cccttttaca aagaacatat tcagggtgtt ttccagccaa caacaataag tagttatatt
    16921 cacctacaga taaagacctt gtagctttac ctgcgctttc tttcatttgc caggtattta
    16981 actgatagcc aaaaatttcg gcagcttcct taagcgttaa acctgcggaa attctcgcct
    17041 ctttaacatc tgccggagtt ggtttagttt gcattgttat tacctcttgt aaataaatca
    17101 gccagaatac cattatcaag atccaataat tctggcaatg taagattcgt atcgagcacc
    17161 aattttttaa ctgtcttttt ccctttaact tgctctttat cacaagattt cacatatgtc
    17221 ttaacatact ttctgaccat atacatctga ttatccatat ctactgtaac aacataagag
    17281 taactaccct tacttttaaa gaaaacatta tcagttttca taaacaaccc catataaaat
    17341 tcacatcaac actttgttat agttttttta taaaacctgc cactttgata agttgatcaa
    17401 cattaagccc tctgataacc atttcataat catcaccatt ttgtagtatt tttcctgcaa
    17461 gtccattatc cgcattaaat cccgttaatg ccccatacca gggagatata gaaagcaact
    17521 gtgataacat ccctttgatt attcgcactt tatcttgttt ttcctgtaat gcgctctcat
    17581 gctccctgag caattcaatt tcatccagtg cattagcaag gattttccga gatatatcat
    17641 tagcaatacc aacaggattg cgcgtaagag atacagtaca gacggaagga ttaccgtgcc
    17701 atacaagact atcaacgcta ccaactataa aaaatctttc acgctccaga cgaataatga
    17761 tggagtattt acgaatttct gtagaagtca gtttaatacg atgctgataa ttatccagtt
    17821 tgttaacacg ccagccgttc ccaagaatac ggcatacaac ttcaaagaca ggctgatatt
    17881 tttctgaaaa tgttgtcatg ctttcaaatc ctttactgcg ctaacgtagc cagcaatcgc
    17941 agagtgtcag ctactcaatc cttttcgggt tcgatgcccg aaaaggattg ctggcgagga
    18001 tatagggggc ttacgccccc gtttcgttat gctctggctt caactcttgc cctgccttca
    18061 acttgttgcc atcctccggc ctggaatttt ccgcttgtgc catattttgc gcggatttcc
    18121 tctatcgtgt gctcttcacg gttccagtaa cttttatgtt cttctggacg ataaaaccat
    18181 tgttttttct ttgccgccca tttacaccct atttctttta atgcttcttt gtgctcgata
    18241 gtatttccac taatccagat ccagttacca ataacctcat aaactaaacc agacaaaccg
    18301 taaagagcct ttaaaacatt ttctagttca tcaccatagt tatatattgc actttgttca
    18361 ggactttggt actgattaat cttatcaaga ttcgccatta aaaaatcgaa tgctgaatta
    18421 accgccttca tcatttcagc acctaccgga ttacggtctg ggtgatattt taatgcagct
    18481 ttcttaaagg cgtttttgat gtctttttca gtcaactggc cagacaagcc aaaaatattt
    18541 aatgcttctt ggatattcat tttgtagctc ccgcccctgt ggggctaaga tgagagggaa
    18601 accatttccc tctcgatgat ttaattatac cttcaattgc aggtattgca agttttttag
    18661 ctctcttttt gaaaaaattt ataattactg tcaggacatc aacattgctt aaaacatttc
    18721 atatccctgt aaataattta accaccacct gaattgatcc caatccgtaa atttcattgc
    18781 tgggattctc gtttcccatc ctttgacaag tgaatccgca agttgtacga tctgacctgt
    18841 tgacatttga acacctctct attgttttga gtgtcgataa agtgatcagg ttgctcgacc
    18901 aacctgatca cacctttata gaatatatgg ctattgcata cattctatat attctttaag
    18961 attcaaaggc tcctcaccta tttttaaatc atcaaaatac ttttcatatt ttttagcata
    19021 atactcaatc agttcaatat cttcttgcgt aatttcccct aattcttcta ttacaccatt
    19081 tttataatct ctaatcttat tgttatctgt aaatactaca atatattttt caccagtctc
    19141 tatatgaatt aatccaatag tacaaagtgt caaagtatta aaaataaaaa tctgttgcgt
    19201 ggttgattca atataaggta agcgagccat ttttaaactc cacttgcttt agatttggat
    19261 taaataaaga gccatataac ggggcaattg ccccgttgag gacttacagc aacgttttgt
    19321 aatgggctaa aagcgcttcc agttcagtcg ctttctttct gaaaacaggg gaatgggact
    19381 ctaccgcatc aatcagtgcg tcaagttctg ttgtgatttc ctgcaaagtg cttaaaaatt
    19441 ctgtaccctc ctggttggtc atccaggaaa caaaggcttt tactgcttgt tcctgcataa
    19501 cttcaatttg ggtaatagcg tctttttgct gatcgccagt tgctttaaat gtggttctaa
    19561 aaataaatgc actcatttta atctcccgcc cctgtggggc taagtggaac ctatttccct
    19621 ttcgatgatt ttattatacc tgcatatgca ggtattgcaa ctatttttca ctattcgaac
    19681 tgaaaaattt tccaccactc acctttaatt ccttcgcgta tgtgctcaat tcagcaaggg
    19741 cactattccg cttcctcacc cactcgctca ctaacgttcg gtcgctaggg ttgcggcgag
    19801 cggatctact ttcctagggt tgtacaccgg aaacgccgaa atttccgatt tccaaactga
    19861 cccccaacag gaaccttttt gtgatctttc tcgatcatat tgttagcagt tagttaagtt
    19921 atgctcgatg gtgggaagat gccgcgttag cggcatgtaa ggcgcgaaat atagttccat
    19981 agaatcacaa atgctaaggc gcgccgatac ccctaatcaa ggacgttcat gtcgccagga
    20041 accgcgtcag ccccctgcta agccaaaatt gcacggaact tacagctact taggtggtag
    20101 gtggtcgtag accgtcgatg ttacagcgat gataggtgaa tgattgttcg tttttaatga
    20161 ccggatatgg tgtctctaat gccccgatgc ttgcgagggg tgctaccttt gaggcgcttg
    20221 cgccgatgtt tttcagttat ccacttgtaa aaggggataa cagtaatgtt tttctttttg
    20281 cttctctttt agtacgtaag taactaaaag agaagcaaaa gagtaattgc gtcatatcgt
    20341 ttgcacttca cttatgatta tccacaagct gtacatcgtt taccctctgg ccataaccta
    20401 tggctgctct ttcctgagca gggagccgct tggaaaacgg aaatatctca cgctaagact
    20461 attttttgta cagagattta ttatggacgt gacgatatac cgccataagg cggtaagtag
    20521 ccagcatcag gccggaatgg gggtaggtga atgtgcgtaa tattcttttt tgcgttgtcg
    20581 agctgcgtcg cgttttgctt tcatcctggc taatccggct gccgctttcc gcgtccagtt
    20641 ctgaactctg atagctataa gtctggcatc accggagtaa atcacttcat caggatctgc
    20701 ctttaacagc ctggcacgtt cccgataaac cagttttaac ggaatgccaa ggatggcaaa
    20761 gaaatcctcg gttacttcaa tgtgtttaca ggcacaccgt gcatcatacg ggttaaacgg
    20821 agccttctct gtaatgatgg cattcaacca aaccaggcga tcacaaccac gactggcgcg
    20881 agaaatggat ttatgtcccg catcgctgcg tgtaaccagt ccggcacgtt cggcaagcgt
    20941 ttcaaggtta tgcctggttt cccacgagcg taaatcgaga tcgtccagaa tagctaacat
    21001 caattcgcaa agagcctcta tgccaaactt gttagcctca ttaacccaat agcggttatg
    21061 ccggatacgg cggcggagtt cgtttttgat tttgatacgc cctgcgaccg tcgtcagcga
    21121 tttaatccag cgattgagga aacggcgtcg cttacgcgac acaacgttat agcatggact
    21181 ttgttttgcc acccctgacg gcgatatgcg ggggattaag gagttgttct tttcagccac
    21241 caccgcccgc acttattgcc ggacagaggt cttgtattga gaaatgcatt tgctataatc
    21301 ctttgcaggc acccagcctg ctttggaccc cctgattatc ttcttggacc ggcaaaatcg
    21361 aagagagata tcagggggtt gttgcattca ggggggctaa aatgcttccc cccttcccct
    21421 ggacgctgtt acgggaacgt cccacccaaa aatccgtaaa aaccgcacga aaatagcatg
    21481 aacccattcg aagatcaatt catccacaaa agatcccgcc gattaccttc ctatttttct
    21541 ttaaacttca tctggttata taattcttaa caaaattgag attaaatact gtacagcata
    21601 taactcacga tattaatgct aatgtacgca tacattagca ttaatatcga aaagaaatcg
    21661 gcgataacaa acggaaaaat aacggaattt aatagggtat ttaccgctcg ccacaaccct
    21721 agcgaccgaa cgttagtgag cgagtgggtg aggaagcgga aaagcgactt gaccaaatgg
    21781 agcacatacg cactgatgcc taagcactca ttcctttaga tattcattta attgagcaac
    21841 tatttttttt acgttatcca gcttagatgc cattcttctt gcttctactt ccgaatcgta
    21901 attagggttt agctctggac tcgtacgttt tcggtgaaaa tcaagaacag cctttgcaaa
    21961 atcatcgtct tctgtataga aataattttc aggataatca agaagttctg cgattttttt
    22021 tacaagttta aaaggtggtt cagttctgcc tacctcgtag cttgaaaatc gagatcgagt
    22081 gttaatacct tccgctacac caagaagttg agataatttt tcttgagata atccagcctc
    22141 catcctcgct tctttcagtc gtttaggcac cataatacaa cctccctttt tttgggaatc
    22201 ccatcttaca gggcgttaca ggcctcaaag tcaacataat gaatcggggt tgattttgaa
    22261 tcaagtaatg ctattatttc tttagattct tgatattgac acgcaagaat tgctgtataa
    22321 aatcacaact gtgtattttt acagttaaca cttaatgagt acgccagcct aagcccggca
    22381 acttaggacg gcggaaaatg ggggcttgtg tccgtaaggt gacgcttatg aatgcatggt
    22441 atcttctata ttgtaataac aacgatttag accgcattgc atatcgttgt aaaactatga
    22501 acgttgtggc tttctgtccg agatacataa agatcacacc tcggaaagat tgcaacgcag
    22561 tacgcaagga agaaaaagtt ttatttccta actacctctt cctccatttt gatgtgaatg
    22621 tcacgcacac atcagcaata acctctattc caggtgcaat tggcttcgta agtttcggtt
    22681 ccacaatatg tactgtaccg gaaaaagtcg ttatcgctat taaatgcgcc aagatactgg
    22741 cgctaaatcc tgatgatgat tccatagagt gccgtaacat cggtagtgac ctactggcag
    22801 aaattcaacg catatcttca atcatctcac cgttagaacg tcaaacggcc ttctccaggc
    22861 ttttacagaa tcagtaaacc agaccttcat tccttctcca gtcgtaccgc tgtgcttttg
    22921 cacacggata tttcatacag gagctataaa ctcatgccga accctatagg ccaggctaaa
    22981 tctacagccg ttatttacat cggcaaaaaa cgctatgaaa cactggcaaa acacgccaga
    23041 gatataagtt atgttgcgga ggtaaatatt cgtccaagtg cctttttgca gtacatgatc
    23101 gacaaattaa gtaatgaagc tcacaacgaa atgttgaaag agctaatgtc aaaagaggat
    23161 aaataaatgc ccaaacccac ctataacgta tacgtcacac aggaagttcc cactgacgaa
    23221 aacggcggta aaaataccta ctggaccaag gttggcgcgg cattcgcgca taacggaaaa
    23281 ccaggactga atattatgct cgttcctggt attgctgttt ccggcaagct ggtactactc
    23341 gaacctaaag aagacgcccc acaaggaaac taatttagcg agggtactat gctttccacc
    23401 gtttcattta tggcggtggc tatgcagtgt gccgccacag ttcacccctc cacatcactg
    23461 gatgttgcgc gggtcgagtc tggttttaat ccctacgcta ttgctgagat catccccaaa
    23521 cgtgaacgcc agccaggcga caagggcttt atctcacata tgccaaaaac taaggaggat
    23581 gcattaagca tcgtcaaaca gatagaggca aaaggtcgtc gctattctgt tggtctgatg
    23641 caaatcacaa gtacaaattt taatagttac gccgtaacgg ctgctgacct gttcaacccg
    23701 tgcacaaatc tttctgtttt tgaaaagatc atcactgatt gctaccagcg aggcggcaca
    23761 ctgaaacggg cgctgagttg ttattactct ggaaatttta ctacaggaca acaaccagaa
    23821 gctgcgttat ccagaaccag ttacatacag cgaattggtt actcacctga aaagccccgc
    23881 tatgtcgttc ctggaaccag ggatgatatc gcaactcaat cggccattct gaatgcaaca
    23941 cccgttgaag ctccgacacg ccctcgcgtt gtctggcctg gcgcaatcgt gcgcggggtt
    24001 cctgctcaac tgcgccagaa aaaggcagat accgtttatt accccgcaca agttatacgc
    24061 ggaaacagat tttcaactga aaaagaggat taaccgatga ttaacaaatt aaaggccatt
    24121 tgcagccgcg catctgctta tattctggct ccgttagtaa gtgttgtcag tcccgttttc
    24181 gcggctgacg gtttcagccg agcaaatacc atcatggaaa aggtttcgaa tggtctgcat
    24241 ggtctggcag ccgtcaccat caccgtcgct gtgatctgga ttggctataa gactctgtgg
    24301 aaaggcgaaa gcctgtcgca gtgcggttac atcatcattg gcggcatttt aattggcgga
    24361 ggcagtgaaa ttggcgctct gctaatgagc taagaggccg aacat
//
