LOCUS       NZ_CP122621             8731 bp    DNA     circular CON 08-JUN-2024
DEFINITION  Escherichia coli strain ETEC4090 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP122621
VERSION     NZ_CP122621.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32549666
            Assembly: GCF_030013105.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8731)
  AUTHORS   Vereecke,N., Van Hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Virotyping and genetic antimicrobial susceptibility testing of
            porcine ETEC/STEC strains and associated plasmid types
  JOURNAL   Front Microbiol 14, 1139312 (2023)
   PUBMED   37143544
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8731)
  AUTHORS   Vereecke,N., Van hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2023) Department of Pathobiology, Pharmacology and
            Zoological Medicine, Universiteit Gent, Salisburylaan 133, Merelbeke
            9820, Belgium
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Trycycler; minimap2; medaka v. v0.5.3; v2.20;
            v1.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 107x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030013105.1-RS_2024_06_08
            Annotation Date                   :: 06/08/2024 05:03:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,702
            CDSs (total)                      :: 5,569
            Genes (coding)                    :: 5,239
            CDSs (with protein)               :: 5,239
            Genes (RNA)                       :: 133
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 99
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 330
            CDSs (without protein)            :: 330
            Pseudo Genes (ambiguous residues) :: 0 of 330
            Pseudo Genes (frameshifted)       :: 120 of 330
            Pseudo Genes (incomplete)         :: 224 of 330
            Pseudo Genes (internal stop)      :: 44 of 330
            Pseudo Genes (multiple problems)  :: 51 of 330
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP122621.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ETEC4090"
                     /isolation_source="feces"
                     /host="Sus scrofa"
                     /db_xref="taxon:562"
                     /plasmid="unnamed1"
                     /geo_loc_name="Belgium"
                     /collection_date="2012"
                     /collected_by="IMM_DI04"
     gene            1..192
                     /locus_tag="QDW69_RS26830"
                     /old_locus_tag="QDW69_26830"
     CDS             1..192
                     /locus_tag="QDW69_RS26830"
                     /old_locus_tag="QDW69_26830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            862..1023
                     /locus_tag="QDW69_RS28570"
     CDS             862..1023
                     /locus_tag="QDW69_RS28570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596883.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRARFI
                     QMLTEFLK"
                     /name="replication initiation protein"
     gene            1516..1743
                     /locus_tag="QDW69_RS26835"
                     /old_locus_tag="QDW69_26835"
     CDS             1516..1743
                     /locus_tag="QDW69_RS26835"
                     /old_locus_tag="QDW69_26835"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_230498631.1"
                     /translation="MLYAIVISTILCPYSKYAIEHIAFNFIKKDFFERRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            complement(1740..3308)
                     /gene="cea"
                     /locus_tag="QDW69_RS26840"
                     /old_locus_tag="QDW69_26840"
     CDS             complement(1740..3308)
                     /gene="cea"
                     /locus_tag="QDW69_RS26840"
                     /old_locus_tag="QDW69_26840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            3568..4005
                     /locus_tag="QDW69_RS26845"
                     /old_locus_tag="QDW69_26845"
     CDS             3568..4005
                     /locus_tag="QDW69_RS26845"
                     /old_locus_tag="QDW69_26845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WP_170997724.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTGRDGRR
                     EFETCELIRAYGELKQNETPERHSARQAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALEQEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     gene            4086..4505
                     /locus_tag="QDW69_RS26850"
                     /old_locus_tag="QDW69_26850"
     CDS             4086..4505
                     /locus_tag="QDW69_RS26850"
                     /old_locus_tag="QDW69_26850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254598.1"
                     /translation="MRRSGGTLYLIALLTAATVLTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLAETISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            4649..4843
                     /locus_tag="QDW69_RS26855"
                     /old_locus_tag="QDW69_26855"
     CDS             4649..4843
                     /locus_tag="QDW69_RS26855"
                     /old_locus_tag="QDW69_26855"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_213218436.1"
                     /translation="MQPGESRRNCQSETAETDFFHTFGSEEDGDLSRKTEVQGNERDLA
                     IKAKTQQQANTPESRPYAF"
                     /name="hypothetical protein"
     gene            complement(5068..5301)
                     /locus_tag="QDW69_RS26860"
                     /old_locus_tag="QDW69_26860"
     CDS             complement(5068..5301)
                     /locus_tag="QDW69_RS26860"
                     /old_locus_tag="QDW69_26860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001555310.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMKLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5308..5838)
                     /locus_tag="QDW69_RS26865"
                     /old_locus_tag="QDW69_26865"
     CDS             complement(5308..5838)
                     /locus_tag="QDW69_RS26865"
                     /old_locus_tag="QDW69_26865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_349239148.1"
                     /translation="MTSRSRQAAYSEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTAALKELLERYQKTTVSTMDTHWQTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            5894..6259
                     /locus_tag="QDW69_RS26875"
                     /old_locus_tag="QDW69_26875"
     CDS             5894..6259
                     /locus_tag="QDW69_RS26875"
                     /old_locus_tag="QDW69_26875"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000124098.1"
                     /translation="MARSAKPRKRKPAPQRSKLPRYVVKLHPDDFFDEEDAEVLRFDNF
                     DDAVECCADLGIPFFLDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGGMY
                     CPECGTVHWPDGVTPPV"
                     /name="hypothetical protein"
     gene            6259..7446
                     /locus_tag="QDW69_RS26880"
                     /old_locus_tag="QDW69_26880"
     CDS             6259..7446
                     /locus_tag="QDW69_RS26880"
                     /old_locus_tag="QDW69_26880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element IS91 family transposase"
                     /protein_id="WP_000937614.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGSVRPVVTESVTKIMACGTTL
                     MGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVFTL
                     PCQYWSLVFHNRWLLAEMSRIAADVILEICHQTDVEPGIFTVIHTWGRDQQWHPHIHLS
                     TTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPELVIPDELAVEGNSKRDWNR
                     FLDTHYRRGWNVNISRVMDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLRYNSHR
                     TKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPVKRRLLEEVVYVITETVRKTA
                     MQIRWRGMYQRLLKVDPLKCILCGSQMRFTGLKRGYRLAELVLMHERLARQQVCG"
                     /name="IS91-like element IS91 family transposase"
     gene            complement(<7636..8355)
                     /locus_tag="QDW69_RS26885"
                     /old_locus_tag="QDW69_26885"
                     /pseudo=""
     CDS             complement(<7636..8355)
                     /locus_tag="QDW69_RS26885"
                     /old_locus_tag="QDW69_26885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(8345..8668)
                     /locus_tag="QDW69_RS26890"
                     /old_locus_tag="QDW69_26890"
     CDS             complement(8345..8668)
                     /locus_tag="QDW69_RS26890"
                     /old_locus_tag="QDW69_26890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(279..365)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.864"
                     /coverage="100.0"
                     /evalue="1.13e-36"
     Typing          376..934
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.257"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4965..5803)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.0"
                     /coverage="99.0"
                     /evalue="3.99e-148"
     Typing          complement(7637..8355)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.167"
                     /coverage="99.0"
                     /evalue="5.75e-155"
ORIGIN
        1 gtgactaaac aggaaaaaac cgcccttaac atggcgcgct ttatcagaag ccagacatta
       61 acgctgctgg aaaagctcaa cgaactggac gcggatgaac aggcagacat ctgtgaatcg
      121 cttcacgacc acgccgatga gctttaccgc agctgcctcg cacgtttcgg ggatgacggt
      181 gaaaacctct gacacatgca gctcccggag acgctcacag cttgtctgtg agcggatgcc
      241 gggagccgac aagcccgtca gggcgcgtca gcgggtttta gcgggtgtcg gggcgcagcc
      301 atgacccagt cacgtatcga tagcggagtg tatactggct taaccatgcg gcatcagtgc
      361 ggattgtatg aaaagtgcgc catgccgggt gtgaaatgcc gcacagatgc gtaaggagaa
      421 aatgcacgtc caggcgcttt tccgcttcct cgctcactga ctcgctacgc tcggtcgttc
      481 gactgcggcg agcggtactg actcacacaa aaacggtaac acagttatcc acagaatcag
      541 gggataaggc cggaaagaac atgtgagcaa aagaccagga acaggaagaa ggccacgccg
      601 caggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      661 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
      721 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
      781 tcgggaagcg tggcgctttc tcatagctca cgctgttggt atctcagttc ggtgtaggtc
      841 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
      901 tccggtaact atcgtcttga gtccaacccg gtaagacacg ccttaacgcc actggcagca
      961 gccactggta accggattag cagagcgaga tttatccaga tgcttacaga gttcttgaag
     1021 tgatggccca actgcggcta cactgaaagg acagtatttg gtatctgcgc tctgctgaag
     1081 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgttggt
     1141 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1201 gatcctttga tcttttctac tgaaccgcga tccccgtcag tttagaagag gaggatggtg
     1261 cgatggtccc tccctgaaca tcacgtatat agttagcctg acatccaaca aggaggttta
     1321 tcgcgaatat tcccacaaaa aatcttttcc tcataactcg atccttataa aatgaaaaga
     1381 atatatggcg aggtttaatt tatgagctta agatactaca taaaaaatat tttatttggc
     1441 ctgtactgca cacttatata tatatacctt ataacaaaaa acagcgaagg gtattatttc
     1501 cttgtgtcag ataagatgct atatgcaata gtgataagca ctattctatg tccatattca
     1561 aaatatgcta ttgaacacat agcttttaac ttcataaaga aagatttttt cgaaagaaga
     1621 aaaaacctaa ataacgcccc cgtagcaaaa ttaaacctat ttatgctata taatctactt
     1681 tgtttggtcc tagcaatccc atttggattg ctaggacttt ttatatcaat aaagaataat
     1741 taaatcccta acacctcatt tatagtatta agtttattct tatcaatata ggagcataga
     1801 attcctgtaa caatagcaat accccaaata cctaatgtag ttccagcaag caagctaaaa
     1861 agtaaagcaa caacataact cacccctgca tctgcagctt tcttctctaa tgtaagaaat
     1921 agtggcttcc agtcacctgt atccttaatt tttaggatat cagataccac atcatatcca
     1981 aaagaaacat gccccgtaat cttcaagtac ttggcaaact gatctaaatg tttagcccag
     2041 tcatcatact tcaccgatgc cagcgcatta aaaatagcat cacgatcggc tttgctgaat
     2101 ttcttattta aaacatcctt gtatttttca aaagcagcga gagcttcatt cacattgccg
     2161 attttcttac ctttagactt atcagcaagt tcctgtgcca ttttcgaata tttttcacca
     2221 tatttttcag tcagcgtttg ataaaagcta actgttgcat caacagcatc cttaatctgt
     2281 gaattaagga gattattctg tgcttttttc aaattttctt cagcttcatg aacacgagcg
     2341 ataccggcat tacgattatt actgacctga gaaatagcct tctggatctg agttatatca
     2401 gcatttatcc ggttaatacg tgtttctgat gctgttacct gtttttgttt ttcttctcta
     2461 atcttaccgg ccccaacccg tcgtctggtt gcttcaaaaa aaggacggtt ctgaagcgga
     2521 tcattggctc ttggtgatag ttttttgacc agttcatcca gttctttata tttagcggat
     2581 gcctgagcca gttcatttcg ttttccagcg agcgttttca tttctgcatc acgggcatgg
     2641 atactggagc ttaaacgaga attgagagtc ttaatctctc catccatttt caccacttca
     2701 gattgtgcag cagaaagttt tttttgggcg atctcaacag ctttagcttc ttcactcaat
     2761 gcagccagtc gtttctcttc agcttcagcc agtttcaact ggcgttctgt ttcagccttc
     2821 tcccgttcaa tctctttacg tcgttgttct gcttcctgaa aagccttttc tgctgcttcc
     2881 gcttctttac gggctttttc ttctgctttc gcaaggcgca aacgctctgc ttccgcctgc
     2941 atagctgcat tattagcatg agcaagctct gttgctgaag gcgtacgtga ggcattgtga
     3001 cgaagagcct cattcacgat atccttcagg cgctgagtca gcgcatccct gtttgccttt
     3061 gctttcgcct gtgcttccgc tgcagctttt gcccgggcag cctgctctgc ctgtgttttc
     3121 tttaattgag cagtagacca tttagcagtt gcatgaatag ctgcagaact ttcactttta
     3181 ctgcctcctt ttccacctcc gccgccagag ccactcccgt caggagtacc attcaaaaga
     3241 gtaataatta cctgcccctt atcatcataa ggaacaccat ctttatagta cgctaccgct
     3301 gtttccatta taaaatcctc tttgactttt aaaacaataa gttaaaaaca aatactgtat
     3361 acataaccac tggttttata tacagcttaa aaattacgcc gctgcgtttt ccctgtcaac
     3421 cctgtggatt ttcatttttg tgaaaacgtt caaaaaaaca gccacccaca ccacgtgcta
     3481 ttcccgcccg atgccacaaa aaccagcaca aacattaccg ttctcagacc tcattatgtt
     3541 ttactgaaac tatgagatga gacatctatg ggacactgtc actttatggc atggcacaca
     3601 ctccgggacg cgctgaaaat gacgggcaga tcgcgctcac agttttaccg tgatatgcgc
     3661 tcaggccttg tcagttaccg taccggcagg gatggacgac gagagtttga aacctgtgaa
     3721 ctgatccggg catacggaga attaaagcag aatgagacac cagaaaggca cagtgcgaga
     3781 caggcagaaa atccacatga tcagcagaca gaacgcattc tccgggagct gaatgagctg
     3841 aaacaatgcc tgacgctgat gcttgaggat aaacaggcac aggatatgga tcgcagacgc
     3901 caggaagcag aacgggaaca gctacaaaat gagatagccc agctcaggca ggcactggaa
     3961 caggaaaaga aacggggatt ctggtccagg ttgttcggtc gctgaacgca gtcagagact
     4021 gatgataaaa tagtcttcgg ataataactc accgagaata aatactttaa ggtagggaga
     4081 cactcatgag acgttccggc ggcacactgt atcttatcgc cctgctgaca gcggcaacgg
     4141 tcctgacagc ctgcacgcca aagggcagtg tggaacaaca tacccggcat tacgtttatg
     4201 catcagacga tggttttgat cctaactttt ccacccagaa ggccgacaca acacgaatga
     4261 tggtgccttt tttccggcag ttctgggata tgggagcaaa ggataaagcg acaggaaaat
     4321 cacggagtga tgtgcagcaa cgcattcagc agtttcacag ccaggaattt ttaaactcac
     4381 tccggggcac aactcaattt gcgggtactg attaccgcag caaagacctt actccgaaaa
     4441 aatccaggct gctggctgag acgatttctg cggtttatct cgatggctac gagggaagac
     4501 agtaagtgga tttaccataa tccctaaatt gtacgcaccg ccagaatacg ttcatcgcga
     4561 tcacggcagc agacaggtaa aaatggcaac aaaccccccg gaaaactgcc gcgatcgcgc
     4621 ccggtaaatt ttaaccacat gcatacctat gcagccaggt gaatcacgca ggaattgcca
     4681 gtcagagacc gctgaaacag atttttttca tactttcgga agcgaagaag acggggattt
     4741 gagcaggaaa acagaagttc aggggaatga gcgcgatctg gcaataaagg caaaaacaca
     4801 gcaacaagca aacacaccag aatcgcgccc gtatgcgttt taacgcgttc ctgtgttttc
     4861 agcaaggtct gactgacgcg cgttcccgat gcccccgaac cagccattat cccaaaaaac
     4921 gcaccggaaa cgcgctgcca acattttagc ctgtctctgg ttaccatccc agcgaagggc
     4981 catccagtgt ccgttcctga atttccagct ggcgctcacg ctccagggat aacgcatgtt
     5041 cgcgttcctg tatcagccgt tcgtccctta tctcctgttc tcgctgtata actggctcaa
     5101 gcgctctgtc cgctcgctca agtgctgcac ctgctgactc aacttcatga cccgctcgtt
     5161 cagcatcgcg ttgtcccgtt gcgtaagcga aaacattttc tgcaattcca cgaaggcgct
     5221 ctcccattcg ttcagccgct gcatatagtc ctgttgcagt tgctctaatg cgttcagcaa
     5281 atgtctttcc agctccgtca tgctctgtca ctcctgcacc agtctcactg cgttcagttt
     5341 gccgttgtcc ttcgtccagt tcgtttcggc tttcatcccc ttcggcaaca ccagaaaacg
     5401 tccgttgctg tcctcgtgat aacgcacgcc ccatgtcttc gcgttcagct tctccagact
     5461 ctctttctgc tcgcggagaa tattcatatt cgcgtttatc ttcatgccct ggtaccacag
     5521 cagactcccg gtcgtggcaa acatcagtac tgagacgata atcagccaca gccagcgttt
     5581 cacgctcatt ttcagcacgg tttgccagtg tgtgtccatc gtgctgacgg tcgttttctg
     5641 atacctctcc agaagttctt tcagggctgc tgtatggtcg ttgatagcgt tcctgattaa
     5701 agattcgttt gaggtcagtt ccctgtttac agattcttcg agcttcctga actcgtcgtt
     5761 cagcattttc tctgtagatt cggctctctc tttcagcttt ttctccgagt aggcagcctg
     5821 gcggctgcgg cttgtcatgg tctggaatta ccgttataaa aaaagataat gtcattgtct
     5881 ttcaggtagt tatatggccc gttcagctaa accccgtaaa cgcaaacccg caccacaaag
     5941 aagcaaactt ccccgctatg ttgtgaaact tcatccggat gatttttttg acgaagaaga
     6001 cgctgaagtt ctgcgctttg ataattttga cgatgccgtt gagtgctgcg ctgacctggg
     6061 tattccgttc tttctggatg caggaaacaa aaagctggtc ttctggtttg ttcgtgtcga
     6121 tgacgaaggg tatccggaaa tagcccgctg tacggagcgg gagtttgcaa ccattcttgc
     6181 cggtatcagt gccggtggta tgtactgccc ggaatgcggc acagttcact ggccggatgg
     6241 cgttacccca cccgtctgat gcttccccgt tttgccgata tttttcagca gggtaaccgc
     6301 tggcttaact ggctggagaa acagccggaa ggttcagtgc gtccggtggt gactgagtca
     6361 gtgacaaaaa tcatggcatg cgggaccacg ctgatgggct acacgcaatg gtgctgttcg
     6421 tcaccggact gttgccacac caaaaaggtc tgcttccggt gtaaaagccg ctcctgtccg
     6481 cactgcgggg tgaaggctgg cgcacagtgg atacagtatc tgctgagcct ggtccccgac
     6541 tgcccgtggc agcatattgt gttcacactt ccctgccagt actggtccct ggtgttccac
     6601 aaccggtggt tactggcaga gatgagccgc attgcagcgg atgtgatact ggaaatctgc
     6661 catcagacag atgtggagcc ggggatattc acggtgatcc acacatgggg gcgtgaccag
     6721 cagtggcatc cgcatatcca tttatcgaca actgccggtg gtgtgacgtc gggccacacc
     6781 tggaaaaatc ttcattttta cgcccgtaag gtgatgagca tgtggcgtta ccggataacg
     6841 cggctactgt cccggaaata cccggagctg gtaataccgg atgaactggc agtggaagga
     6901 aacagcaaac gggactggaa tcgcttcctg gacacgcatt accgccgcgg ctggaatgtc
     6961 aacatatcca gggtgatgga taacgccaca catgtggcgg tgtacttcgg ctcttacctg
     7021 aaaaagccac cggtgccgat gagtcggctg gagcattatg ccggtcagga tgaaatcggt
     7081 ctgcgttaca acagtcaccg tacaaaacgg gaagaatacc tgttgatgag tggagatgag
     7141 ttcatggaaa ggttctcctg gcatgtagca gataaggggt tccgtatggt gaggtactac
     7201 ggtttcctga gtccggtgaa gcgccgctta ctggaagaag ttgtgtacgt cataacggag
     7261 acggtgagaa aaacggcgat gcaaatcagg tggagaggga tgtatcagag gttactgaag
     7321 gttgacccgc tgaagtgcat tctgtgcgga agtcagatgc gttttacggg gctgaagcgg
     7381 ggttaccgac tggcagagct ggtcctgatg catgagcgac tggcacgaca gcaggtgtgc
     7441 ggctgagagc cgcagagggg aagttgcgtc cattttaccg gaaacggagc aaaaaaccgc
     7501 cattcatacc ctgtatcaat cagtgtcatc ctgtttaata gtcgtttccg ctcatatggt
     7561 gcacaagggg tgttgaagaa acatccgttt tgtggtgctt ttttagtctt ttggggattt
     7621 aaattcctat cgatgaactc cgcgcccgtc tgcaaaagat tgctcataaa acgctccttt
     7681 cagtctgata ttcctcccgc cgttcgggtc tgcaatgctg atactgcttc gcgtcaccct
     7741 gaccacttca agtcccgcct ccgtgagcgc ctgaatcaca tcctgacggc cttttatctc
     7801 tccggcatgg taaagcgcgt ctatcccgcg tgtaacgccc tcagcaatcg cctgtttcgt
     7861 ttccggcaga ttatcaggga gtgtcagcgt cctgcggttc tccggggcgt tcgggtcatg
     7921 cagcccgtaa tggtgattaa ccagcgtctg ccaggcatca attctaggcc tgtctgcgcg
     7981 gtcgtagtac ggctggaggc gttttccggt ctgtagctcc atgttcggaa tgacaaaatt
     8041 cagctcaagc cgtcccttgt cctggtgctc cacccacagg atgctgtact gatttttttc
     8101 gagaccgggc atcagtacac gctcaaagct cgccatcact ttttcacgtc ctcccggcgg
     8161 cagctccttc tccgcgaacg acagaacacc ggacgtgtat ttcttcgcaa atggcgtggc
     8221 atcgatgagt tcccggactt cttccggatt accctgaagc accgttgcgc cttcgcggtt
     8281 acgctccctc cccagcaggt aatcaaccgg accactgcca ccaccttttc ccctggcatg
     8341 aaatttaact atcatcccgc gccccctgtt ccctgacggc cagacgcagc cggcgcagct
     8401 catccccgat ggccatcagt gcggccacca cctgaacccg gtcaccggaa gaccactgcc
     8461 cgctgttcac cttacgggct gtctgattca ggttatttcc gatggcggcc agctgacgca
     8521 gtaacggcgg tgccagtgtc ggcagtttcc cggaacgggc aaccggctcc cccaggcaga
     8581 cccgccgcat ccataccgcc agttgtttac cctcacagcg ttcaagtaac cgggcatgtt
     8641 catcatcagt aacccgtatt gtgagcatcc tctcgcgttt catcggtatc attaccccat
     8701 gaacagaaat cccccttaca cggaggcatc a
//
