LOCUS       NZ_CP122607            19834 bp    DNA     circular CON 08-JUN-2024
DEFINITION  Escherichia coli strain ETEC6334 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP122607
VERSION     NZ_CP122607.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32549675
            Assembly: GCF_030013025.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19834)
  AUTHORS   Vereecke,N., Van Hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Virotyping and genetic antimicrobial susceptibility testing of
            porcine ETEC/STEC strains and associated plasmid types
  JOURNAL   Front Microbiol 14, 1139312 (2023)
   PUBMED   37143544
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19834)
  AUTHORS   Vereecke,N., Van hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2023) Department of Pathobiology, Pharmacology and
            Zoological Medicine, Universiteit Gent, Salisburylaan 133, Merelbeke
            9820, Belgium
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Trycycler; minimap2; medaka v. v0.5.3; v2.20;
            v1.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 169x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030013025.1-RS_2024_06_08
            Annotation Date                   :: 06/08/2024 05:20:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,374
            CDSs (total)                      :: 5,252
            Genes (coding)                    :: 4,943
            CDSs (with protein)               :: 4,943
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 98 of 309
            Pseudo Genes (incomplete)         :: 202 of 309
            Pseudo Genes (internal stop)      :: 49 of 309
            Pseudo Genes (multiple problems)  :: 35 of 309
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP122607.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..19834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ETEC6334"
                     /isolation_source="feces"
                     /host="Sus scrofa"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="Netherlands"
                     /collection_date="2021"
                     /collected_by="IMM_DI04"
     gene            1..453
                     /locus_tag="QDW51_RS26335"
                     /old_locus_tag="QDW51_26335"
     CDS             1..453
                     /locus_tag="QDW51_RS26335"
                     /old_locus_tag="QDW51_26335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000069427.1"
                     /translation="MSNNIKYGSRILDMGVLLEMKALVNANGGVSRCIMECRQLACEVR
                     HSQQLLDKLSCIDDWLCRFSSIMYHRFDKEQIQWREDVDRDYPREKVYIFSPDNVSVHN
                     NDIKDSANNTSGITVSITASKICAGQKEFDAFLKLAEKLRENPTGN"
                     /name="hypothetical protein"
     gene            481..1119
                     /gene="trbC"
                     /locus_tag="QDW51_RS26340"
                     /old_locus_tag="QDW51_26340"
     CDS             481..1119
                     /gene="trbC"
                     /locus_tag="QDW51_RS26340"
                     /old_locus_tag="QDW51_26340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin assembly
                     protein TrbC"
                     /protein_id="WP_001104248.1"
                     /translation="MNWKTFALFALTAGMHITVHASDNVNTPENRQFLKQQENLSRQLR
                     EKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPEEG
                     LKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGIRSV
                     PALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVAHDLLAGKGDSGK"
                     /name="trbC"
     gene            1116..2084
                     /locus_tag="QDW51_RS26345"
                     /old_locus_tag="QDW51_26345"
     CDS             1116..2084
                     /locus_tag="QDW51_RS26345"
                     /old_locus_tag="QDW51_26345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_258056481.1"
                     /translation="MKRILPLILALVAGMALADSNSDYRAGSDFAHQIKGQGSSSIQGF
                     KPQESIPGYNANPDETKYYGGVTAGGDSGLKNDGTTEWATGETGKTITESFMNKPKDIL
                     SPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEISRSEYTNYTCERDLQVEQYCTRTARM
                     ELQGSTTWETRTLEYEMSQLPAREVNGQYVVSITSPVKGMASVPSGGGSQDIIADHQGH
                     QAIIEQRTQDSNIRNDVKHQVDNMVTEYRGNIGDTQSSIRGEENIVGRQYSELQNHHKT
                     EALSQYNKYNEEKSGQERMPGADSPQELMKRAKEYQDKHKQ"
                     /name="hypothetical protein"
     gene            2099..2602
                     /locus_tag="QDW51_RS26350"
                     /old_locus_tag="QDW51_26350"
     CDS             2099..2602
                     /locus_tag="QDW51_RS26350"
                     /old_locus_tag="QDW51_26350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000605510.1"
                     /translation="MIMKNLAHIALVTVIQFIACYLADWGSAETVFILCFIVLWQGLFI
                     WLFSQIRKKRHVSDEFKFSKGVWYILMPVSSLLSPLLSLMVFIIGTLYELRRISGCVSV
                     REWMQSQVNEQTNEDLHLDFDDVNFDYVDFYRTNPATGLAMHGGFDSAGNTFGTCWQDY
                     DDRR"
                     /name="hypothetical protein"
     gene            2634..3365
                     /gene="traT"
                     /locus_tag="QDW51_RS26355"
                     /old_locus_tag="QDW51_26355"
     CDS             2634..3365
                     /gene="traT"
                     /locus_tag="QDW51_RS26355"
                     /old_locus_tag="QDW51_26355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="WP_000850424.1"
                     /translation="MKTKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIWL
                     EPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKADKM
                     DLRESQGWLNRGYEGAAVGAALGAGITGYNSSSAGATLGVGLAAGLVGMAADAMVEDVN
                     YTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNANKVNL
                     KFEEAKPVLEDQLAKSIANIL"
                     /name="traT"
     gene            3568..4305
                     /locus_tag="QDW51_RS26360"
                     /old_locus_tag="QDW51_26360"
     CDS             3568..4305
                     /locus_tag="QDW51_RS26360"
                     /old_locus_tag="QDW51_26360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000199900.1"
                     /translation="MTSDNIAEYVGENNQFTEKPVTEYPPVIQDNTVTTKDYLYSNNID
                     TDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILEKISFPEKSGSNLLKPEAVSCNL
                     LSLCVLAPVFCTPKKLAEVSREIIVASAQEIAWTPGSSRIDGHQAENITWAISSQSPAD
                     KYDLINKTSRVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIVTEKV
                     SSGRAAGGYHHRHKGYTWSVSVN"
                     /name="hypothetical protein"
     gene            4356..>4892
                     /locus_tag="QDW51_RS26365"
                     /old_locus_tag="QDW51_26365"
                     /pseudo=""
     CDS             4356..>4892
                     /locus_tag="QDW51_RS26365"
                     /old_locus_tag="QDW51_26365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="TraD N-terminal domain-containing protein"
                     /name="TraD N-terminal domain-containing protein"
     gene            4911..5657
                     /gene="traX"
                     /locus_tag="QDW51_RS26370"
                     /old_locus_tag="QDW51_26370"
     CDS             4911..5657
                     /gene="traX"
                     /locus_tag="QDW51_RS26370"
                     /old_locus_tag="QDW51_26370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="WP_000205711.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLMV
                     LDHINLIFQLKQDWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQFAY
                     YLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAGLLM
                     LAVSHRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSCAGKS
                     LPRFWPGDFFPVFYACHLAVLGVLAL"
                     /name="traX"
     gene            5716..6576
                     /locus_tag="QDW51_RS26375"
                     /old_locus_tag="QDW51_26375"
     CDS             5716..6576
                     /locus_tag="QDW51_RS26375"
                     /old_locus_tag="QDW51_26375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_000704522.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLIILCHGFCGIRNVLLPCF
                     ANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDIISVINWAEKQECIDNQRIGLW
                     GTSLGGGHVFSAAAQDQRVKCIVSQLAFADGDVLVTGEMNESERASFLSTLNKMAEKKK
                     NTGKEMFVGVTRVLSDDESKVFFEKIKARHPEMDIKIPFLTVMETLQYKPAESAASVQC
                     PVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGAFFERVVAVQTQW
                     FKQYL"
                     /name="alpha/beta hydrolase"
     gene            6679..7239
                     /locus_tag="QDW51_RS26380"
                     /old_locus_tag="QDW51_26380"
     CDS             6679..7239
                     /locus_tag="QDW51_RS26380"
                     /old_locus_tag="QDW51_26380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="WP_000840464.1"
                     /translation="MAEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLAE
                     KAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRDVL
                     LEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEAYAA
                     ARLDKIRRQNRIKAELQAVLDEK"
                     /name="fertility inhibition protein FinO"
     gene            7370..7579
                     /locus_tag="QDW51_RS26385"
                     /old_locus_tag="QDW51_26385"
     CDS             7370..7579
                     /locus_tag="QDW51_RS26385"
                     /old_locus_tag="QDW51_26385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001297500.1"
                     /translation="MNGFRNSSRNGQVWRWQRTGHRAVNLEIRGFWMEAAEAWCQAAGI
                     APRADWRRFARLRAERCRKYGRKE"
                     /name="hypothetical protein"
     gene            7991..8617
                     /locus_tag="QDW51_RS26390"
                     /old_locus_tag="QDW51_26390"
     CDS             7991..8617
                     /locus_tag="QDW51_RS26390"
                     /old_locus_tag="QDW51_26390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685921.1"
                     /GO_function="GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="WP_000794191.1"
                     /translation="MKNMMRNEALRIALIDYENCCNLRNISLADYTDLILFAGPLQQTV
                     VLPADTWPDNIRVSIRQIDNISKNNVDFHLVMELGRMTCCSAGNNTWHIISNDKGYDGI
                     IHTLQKRGFRCERIAPEHAESHLPVSHLIVPEGDVIIRWANRMQQASGSDVRNLPVSVE
                     GFNNYLKSHMRGEWHKERAVSIRSELVRRGVIKIQADKIVWLTRR"
                     /name="NYN domain-containing protein"
     gene            8769..9359
                     /locus_tag="QDW51_RS26395"
                     /old_locus_tag="QDW51_26395"
     CDS             8769..9359
                     /locus_tag="QDW51_RS26395"
                     /old_locus_tag="QDW51_26395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="WP_001375082.1"
                     /translation="MNLIIFIVVVIIIAVLSVRIIFRRSKNCPPALIRLHAAGIRPGEA
                     ERILVSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRPRS
                     RQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQAGI
                     PLLRSHDARKLLQMTGEWLNTTGAAQQSPEHRS"
                     /name="DUF2726 domain-containing protein"
     gene            9599..9847
                     /locus_tag="QDW51_RS26400"
                     /old_locus_tag="QDW51_26400"
     CDS             9599..9847
                     /locus_tag="QDW51_RS26400"
                     /old_locus_tag="QDW51_26400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="WP_000083839.1"
                     /translation="MSQTENAVTSSSGTKRAYRKGNPLTLAERQQASLARKRATHKELR
                     VFIPAALKAQLQVMCEAEGVTQAEMIAELIKQKSAFS"
                     /name="replication regulatory protein RepA"
     gene            10092..10166
                     /gene="tap"
                     /locus_tag="QDW51_RS26405"
                     /old_locus_tag="QDW51_26405"
     CDS             10092..10166
                     /gene="tap"
                     /locus_tag="QDW51_RS26405"
                     /old_locus_tag="QDW51_26405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /GO_process="GO:0006276 - plasmid maintenance [Evidence
                     IEA]; GO:0031556 - transcriptional attenuation by ribosome
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="WP_001365705.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
                     /name="tap"
     gene            10159..11016
                     /gene="repA"
                     /locus_tag="QDW51_RS26410"
                     /old_locus_tag="QDW51_26410"
     CDS             10159..11016
                     /gene="repA"
                     /locus_tag="QDW51_RS26410"
                     /old_locus_tag="QDW51_26410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /GO_process="GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator RepA"
                     /protein_id="WP_042634321.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSRF
                     DFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECGLA
                     TESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTLALFAALDVSED
                     AVAAARRSRVEWENKQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIKRARA
                     HRDANRERQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRNYSRLA
                     TASP"
                     /name="repA"
     gene            11718..11858
                     /locus_tag="QDW51_RS26415"
                     /old_locus_tag="QDW51_26415"
     CDS             11718..11858
                     /locus_tag="QDW51_RS26415"
                     /old_locus_tag="QDW51_26415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001384252.1"
                     /translation="MAESRYGLAGLGVGKVKSINQYRLTPGFGGFTPVSYETTECRLTC
                     R"
                     /name="hypothetical protein"
     gene            12015..13052
                     /locus_tag="QDW51_RS26420"
                     /old_locus_tag="QDW51_26420"
     CDS             12015..13052
                     /locus_tag="QDW51_RS26420"
                     /old_locus_tag="QDW51_26420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="WP_032083970.1"
                     /translation="MRPAIFEPQQIIEAGLALQAEGRNITGFALRNRVGGGNPGRLKQV
                     WDEYISSQTEVTTGPVAELPVEVAEEVKAVSSALAERISQLATELNDKAVRAAERRVAE
                     VTRAAGEQTTQAERELADAAQTVDDLEETLDTLREEHSATLAALDDSRRKEQAQAVELA
                     QLRERLAATERLLGESEKAGKEAAEQHRQQQADLQKRLDAAGNKLAEATTRHATDLQGV
                     REQYAGEVKELKAQHARNESDLQRRLDAAEKKLAEAITNHAIEIKGIKEQHTGEIKELK
                     AQHARSEKELQNRLDTELKARQAALDKALRDATDAREAKATLTGELQSLKTHNDQLAAL
                     LAGKK"
                     /name="DNA-binding protein"
     gene            13228..13497
                     /gene="yacA"
                     /locus_tag="QDW51_RS26425"
                     /old_locus_tag="QDW51_26425"
     CDS             13228..13497
                     /gene="yacA"
                     /locus_tag="QDW51_RS26425"
                     /old_locus_tag="QDW51_26425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
                     /protein_id="WP_000079937.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQH
                     NTWFRDQVAAGRQQLKHGDVLPHDMVESSAAAWRDEMSRKVAGK"
                     /name="yacA"
     gene            13497..13775
                     /gene="yacB"
                     /locus_tag="QDW51_RS26430"
                     /old_locus_tag="QDW51_26430"
     CDS             13497..13775
                     /gene="yacB"
                     /locus_tag="QDW51_RS26430"
                     /old_locus_tag="QDW51_26430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
                     /protein_id="WP_244461648.1"
                     /translation="MEIFWTMLASQDRKRIREYIAEQNLTAAIELDERIGYSASSLAGQ
                     PYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
                     /name="yacB"
     gene            14039..14539
                     /locus_tag="QDW51_RS26435"
                     /old_locus_tag="QDW51_26435"
     CDS             14039..14539
                     /locus_tag="QDW51_RS26435"
                     /old_locus_tag="QDW51_26435"
                     /inference="COORDINATES: protein motif:HMM:NF017519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="WP_282833034.1"
                     /translation="MLIKNKSEIHLGPHFMLNYELLQPGDIILERGYAWYSEKIAKHTN
                     SRYSHAMIYVGGTIIEATRGGGVYSRIPNRSTVRDISDFKVLRLKEFPGKDILQVICDH
                     ARSLCGSQYSVSQALKVKGPDFLRKFANDSRKQFCSRLVAQCYQKAGIQLTENINFCSS
                     IGI"
                     /name="YiiX/YebB-like N1pC/P60 family cysteine hydrolase"
     gene            complement(14712..15899)
                     /locus_tag="QDW51_RS26440"
                     /old_locus_tag="QDW51_26440"
     CDS             complement(14712..15899)
                     /locus_tag="QDW51_RS26440"
                     /old_locus_tag="QDW51_26440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="WP_000937591.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGSVRPVVIESVTKIMACGTTL
                     MGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVFTL
                     PCQYWSLVFHNRWLLAEMSRIAADVIQEICRQADVVPGIFTVIHTWGRDQQWHPHIHLS
                     TTAGGVTPDHTWKNLHFYARKVMSMWRYRITWLLSRKYPELVIPDALAVEGSSRRDWNR
                     FLDSHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLRYNSHR
                     TKREEYLVMSGDEFMERFSWHVADKGFRMVRYYGFLSPVKRRLLEDVVYVITETVRKTA
                     MQIRWRGMYQRLLKVDPLKCILCGCQMRFTGLKRGYRLTELVLMHEPLAQQRVCG"
                     /name="IS91 family transposase"
     gene            complement(15899..16264)
                     /locus_tag="QDW51_RS26445"
                     /old_locus_tag="QDW51_26445"
     CDS             complement(15899..16264)
                     /locus_tag="QDW51_RS26445"
                     /old_locus_tag="QDW51_26445"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_282833035.1"
                     /translation="MPRSAKPRKRKPAPQRSKLPRYVVKLHDDDFFDEEDAEALRFDNF
                     DDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGGMY
                     CPECGTVHWPDGVPPPF"
                     /name="hypothetical protein"
     gene            complement(16489..16883)
                     /locus_tag="QDW51_RS26450"
                     /old_locus_tag="QDW51_26450"
                     /pseudo=""
     CDS             complement(16489..16883)
                     /locus_tag="QDW51_RS26450"
                     /old_locus_tag="QDW51_26450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /name="plasmid partitioning/stability family protein"
     gene            complement(16883..17842)
                     /locus_tag="QDW51_RS26455"
                     /old_locus_tag="QDW51_26455"
     CDS             complement(16883..17842)
                     /locus_tag="QDW51_RS26455"
                     /old_locus_tag="QDW51_26455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /GO_function="GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="WP_000921958.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAGK
                     VYNYVVDGKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVTLP
                     VTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKKDKV
                     NKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKASTPCNY
                     FIADELIKNRHDNEYLQTLINDVAEIKNITHVIDSEVKSLAESIRQEISTFSGMNRIYL
                     TGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
                     /name="plasmid segregation protein ParM"
     gene            18257..>18382
                     /locus_tag="QDW51_RS26460"
                     /old_locus_tag="QDW51_26460"
                     /pseudo=""
     CDS             18257..>18382
                     /locus_tag="QDW51_RS26460"
                     /old_locus_tag="QDW51_26460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134314.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            <18377..18562
                     /locus_tag="QDW51_RS26465"
                     /old_locus_tag="QDW51_26465"
                     /pseudo=""
     CDS             <18377..18562
                     /locus_tag="QDW51_RS26465"
                     /old_locus_tag="QDW51_26465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /name="DUF905 family protein"
     gene            18626..>19504
                     /locus_tag="QDW51_RS26470"
                     /old_locus_tag="QDW51_26470"
                     /pseudo=""
     CDS             18626..>19504
                     /locus_tag="QDW51_RS26470"
                     /old_locus_tag="QDW51_26470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /name="ParB/RepB/Spo0J family partition protein"
     gene            <19503..19805
                     /locus_tag="QDW51_RS26475"
                     /old_locus_tag="QDW51_26475"
                     /pseudo=""
     CDS             <19503..19805
                     /locus_tag="QDW51_RS26475"
                     /old_locus_tag="QDW51_26475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /name="ATP-binding protein"
     Typing          10160..11016
                     /name="IncFIA"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.902"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          533..1116
                     /name="MPF_F"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="97.436"
                     /coverage="92.0"
                     /evalue="3.5900000000000003e-124"
ORIGIN
        1 atgagtaata atataaaata tggctccaga atacttgata tgggtgttct gctggagatg
       61 aaagcgcttg tgaatgcaaa tggtggtgtg tcacggtgca ttatggagtg tcggcagctt
      121 gcctgtgagg tccgtcattc gcaacaatta ctggataagc tgtcatgtat tgacgactgg
      181 ttgtgccggt tttcctcaat aatgtatcac cgttttgata aagaacagat tcagtggcgg
      241 gaggatgtgg acagggatta tcccagagaa aaggtttata ttttcagtcc cgataatgtc
      301 tccgtccata ataatgatat taaagactct gccaataaca catcaggaat aaccgtgtcg
      361 attacagcca gtaaaatatg cgcaggtcag aaagagtttg atgctttttt aaaactggca
      421 gaaaaattga gagaaaaccc tacgggtaat taaattgtaa caatgccatc aggatatatc
      481 atgaactgga aaacatttgc gctttttgcc ctgactgcag gaatgcatat cactgttcat
      541 gcctcagaca atgtgaacac tcctgaaaac cgccagttcc tgaagcagca ggaaaattta
      601 agcagacaac tgcgtgaaaa acctgaccat cagctgaagg cctgggcgga gaaacaggtg
      661 ctggagaacc cccttcagcg ttcagataac catttcctgg atgagctggt tcgtaaacag
      721 caggcttcgc aggacgggaa accccggcag ggggccctgt attttgtgtc gttttccatt
      781 cccgaagagg ggctgaaacg gatgctgggc gaaacccggc actacggtat acccgccaca
      841 ctgcggggca tggtgaacaa tgacctgaag accaccgctg aagccgtgct gtccctggtg
      901 aaagacggcg cgactgatgg tgttcagatc gacccgacgc tgttttcaca gtacggcatt
      961 cgcagtgtac cggcactggt ggtgttctgc agtcagggat acgacatcat ccggggaaac
     1021 ctgcgggtcg gccaggcgct ggagaaagtg gccgccacgg gtgactgccg gcaggtggct
     1081 catgatttac tggcagggaa aggagattcc gggaaatgaa acgtatttta cctctgatac
     1141 tggctctggt tgccggcatg gcactggctg acagcaacag tgattaccgg gccggctctg
     1201 attttgctca ccagatcaaa ggacagggaa gcagcagtat tcagggcttt aagccacagg
     1261 agagtatccc cggctataac gcgaatccgg acgagacaaa atattacggt ggcgtgactg
     1321 ccggcgggga tagcggcctg aaaaatgacg gcaccacgga atgggcgacc ggtgaaaccg
     1381 gaaaaaccat tacagagtca ttcatgaaca agccgaaaga cattctttca ccggatgcac
     1441 cgtttatcca gaccggcagg gatgtggtga accgggctga cagcattgtg ggaaacaccg
     1501 ggcagcagtg cagtgcgcag gagattagcc ggagtgaata cacgaattac acctgtgagc
     1561 gggatttaca ggtggagcag tactgcacac gaactgcccg tatggagctg caggggagta
     1621 ccacatggga aacccggacg ctggagtatg aaatgagtca gctacctgca cgtgaagtga
     1681 atggtcagta tgttgtctcc attacctctc ccgttaaagg aatggcgagc gtaccttcgg
     1741 ggggtggcag ccaggatatt attgctgatc atcaggggca tcaggccatt attgagcaaa
     1801 gaacgcagga cagtaatatc cgtaatgatg taaaacatca ggttgataat atggtcacag
     1861 aataccgagg caatatcgga gatactcaga gcagcatacg tggcgaagaa aatattgtag
     1921 gaagacagta ttctgaacta caaaatcacc ataaaacaga ggcattatct cagtataata
     1981 aatataatga agaaaaatcg ggtcaggaaa gaatgcccgg ggctgacagt ccacaggaac
     2041 tgatgaaaag agcaaaggag tatcaggata agcacaagca ataatattgt atggggagat
     2101 gataatgaaa aatttagctc atattgctct ggtgacggtg atacagttca ttgcctgtta
     2161 tctggctgat tgggggagtg ccgaaacagt ttttatcctg tgctttattg tactatggca
     2221 gggattgttt atatggttat tttctcaaat acgaaaaaaa cggcatgtgt cggatgagtt
     2281 taaattcagt aaaggcgttt ggtatatact gatgccagta tcttccttac tctctccgtt
     2341 attaagcctg atggttttta tcattggtac actatacgaa ttaaggcgaa ttagtggttg
     2401 tgtaagtgta agagaatgga tgcaatctca ggtaaatgaa caaactaatg aagatttgca
     2461 tctggatttt gatgatgtga attttgatta tgtggatttt tatcgtacta atccggcaac
     2521 aggattagct atgcatggag gatttgattc agcaggaaat acttttggga cttgttggca
     2581 ggactatgat gacaggcgat aagaaaactg tggtttgtaa taaaggtaat ttgatgaaaa
     2641 caaaaaaatt gatgatggtt gcactggtca gttccactct ggccctttca gggtgtggtg
     2701 cgatgagcac agcaatcaag aagcgtaacc ttgaggtgaa gactcagatg agtgagacca
     2761 tctggcttga acccgccagc gaacgcacgg tatttctgca gatcaaaaac acgtctgata
     2821 aagacatgag tgggctgcag ggcaaaattg ctgatgctgt gaaagcaaaa ggatatcagg
     2881 tggtgacttc tccggataaa gcctactact ggattcaggc gaatgtgctg aaggccgata
     2941 agatggatct gcgggaatct cagggatggc tgaaccgtgg ttatgaaggc gcagcagttg
     3001 gtgcagcgtt aggtgccggt atcaccggat ataattccag ttctgccggt gccacactcg
     3061 gtgtgggcct tgctgccggt ctggtgggta tggctgcaga tgcgatggtg gaagatgtga
     3121 actataccat gatcacggat gtgcagattg cagagcgtac taaggcaacg gtgacaacgg
     3181 acaatgttgc cgccctgcgt cagggcacat caggtgcgaa aattcagacc agtactgaaa
     3241 caggtaacca gcataaatac cagacccgtg tggtctcaaa tgcgaacaag gttaacctga
     3301 aatttgaaga ggcgaagcct gttctcgaag accagttggc caaatcaatc gcaaatattc
     3361 tctgaacctc gtcaggaggc tggtctgacc ggcctccttg ctgacgcctc gcgttgctcg
     3421 ttgtccaacc ccgggatgta acaaaataaa ttttgtcacc tcccggcgtt tccgaaataa
     3481 atatgaaaac actgagcggt tgtgcaaatt ctttgtgcga ccgtgaagtg ttgccggcga
     3541 agccgggatc ttccggagaa agtcattatg acatcagaca atatcgctga atatgttggt
     3601 gagaataatc aatttactga gaagcctgta actgaatacc ccccggtgat acaggataat
     3661 acagtgacca ctaaagacta tctgtacagc aataatattg atactgacct tttagtggtt
     3721 atcggagggc ttattgttat attgtttctc atgatgctta tcatgtactt tattaattct
     3781 ctcaggcata aggaattaac tgaaatactg gaaaaaatat cttttcctga aaaatccggg
     3841 agcaatttgc ttaaaccgga ggcggtcagc tgtaatttgt tgtccctgtg tgtgctggca
     3901 ccggtattct gtacacccaa aaagctggct gaagtttcca gggagatcat cgtggcgagt
     3961 gcgcaggaaa ttgcatggac gccggggagc agtcgtattg atggccacca ggcggagaat
     4021 atcacctggg cgatttcatc gcagtctcct gccgataagt atgatctcat caataaaacg
     4081 tcccgtgttt ctcctgacag tggccgtttt tatgaaccgg acacagttct ggatcaccgt
     4141 gaacgggtac gcctttttat tcagatccag tttccgcatg ggagtgactg gtctgaagta
     4201 ttgcgcctga ctgacattgt tacagagaag gtctcttccg gcagggctgc aggcgggtat
     4261 caccaccggc ataaagggta cacctggtct gtttctgtga attaacccca catcatcctt
     4321 ttctgccctg ttccagattt tcccggagtt tcatcatgag ttttaacgca aaggatatga
     4381 cccagggcgg tcagattgcg tccatgcgta tccgcatgtt cagccagatc gccaatatca
     4441 tgctttactg cctgtttatt tttttctgga tactcgttgg tctgatttta tgggtaaaaa
     4501 taagctggca gacgtttgtg aacggctgta tttactggtg gtgtaccacg ctggaaggca
     4561 tgcgggattt aatcaggtcc cagccagtat atgagattca gtattacggc aaaaccttcc
     4621 ggatgaacgc tgcccaggta ctgcatgata aatatatgat ctggtgcggt gagcagctat
     4681 ggtccgcatt cgttctggcg tctgtcgtgg ccctggttat ttgcctgatc accttctttg
     4741 ttgtctcctg gattctgggg cgtcagggta aacaacagag cgaaaatgaa gtcacaggtg
     4801 gtcgtcagct gacagacaat ccgaaagacg ttgcccggat gctgaaaaaa gacggcaagg
     4861 attccgatat ccggattggc gacctgccga ttacaggaag acaaacattg atgacaacgg
     4921 ataacacgaa cacgacacgt aacgattcac tggctgcacg gaccgatacc tggttgcagt
     4981 cgtttctggt ctggtcaccc ggacagcggg atatcatcaa aacggtggca ctggtgctga
     5041 tggtactgga ccacatcaat ctgatattcc agctgaagca ggattggatg tttctggccg
     5101 ggcgaggagc ctttccgctg tttgccctgg tgtgggggct gaatctgtcc cgtcatgcgc
     5161 atatccggca accggccatt aaccggctgt ggggatgggg aattattgct cagttcgcat
     5221 attatctggc cggctttccc tggtatgagg ggaatatcct gtttgccttt gcagtggcgg
     5281 cacaggtgct gacgtggtgt gaaacgcgca gtgggtggcg tacagcagcg gccattcttc
     5341 tgatggccct gtgggggcct ttgtccggca ccagttacgg cattgccggg ctgctgatgc
     5401 tggcagtcag ccaccggctg taccgggcgg aagacagagc ggaacgtctg gcgctggtcg
     5461 cctgcctgct ggctgttatt ccggcgctta accttgccac cagtgatgca gcggcggtgg
     5521 ccggtcttgt gatgacggtg ctgaccgttg gtctggtgtc gtgtgcagga aaatcattac
     5581 cccgtttctg gcccggggat tttttcccgg tattctacgc ctgtcatctg gctgtgctgg
     5641 gcgttctggc gttgtgactg gtatgatact gacgctgatt ctgattatta ataaacaaca
     5701 taacacagga tatttatgaa aattacggat cacaaactct ctgaaggtat tgccctgaca
     5761 tttcgtgttc ctgaaggaaa tataaaacat cctttgatta tcttatgcca tgggttttgc
     5821 ggtatcagga atgttctgct cccttgcttt gcgaatgcct tcacagaagc aggattcgca
     5881 acgattactt ttgattatcg aggattcggt gagagtgatg gagaacgagg gcgtctggtt
     5941 cctgcaatgc agaccgaaga tattatttca gttatcaatt gggcagaaaa gcaggaatgt
     6001 attgataatc agcgtattgg gttatgggga acatcgctgg gaggggggca tgtcttcagt
     6061 gcagctgcgc aggaccaacg cgtaaaatgt attgtcagcc agctggcttt tgctgacggt
     6121 gatgttctgg ttaccggaga gatgaatgaa tcggagagag cgtcttttct gtccacgctg
     6181 aataaaatgg cagaaaagaa gaaaaacacc gggaaagaga tgtttgtggg ggttacacgg
     6241 gtactaagtg atgatgaatc gaaggtattc ttcgagaaaa tcaaagcccg gcatcccgaa
     6301 atggatataa aaataccttt ccttacggtc atggaaacgt tgcaatataa accggcagag
     6361 agtgcagcca gcgtccagtg cccagttttg gttgttattg ccggccagga tagcgttaat
     6421 ccaccggaac agggaagagc attatatgat gctgttgctt ctggtactaa ggaattatac
     6481 gaggaagctg atgcatgcca ttatgatatt tatgaaggcg cgttttttga acgtgtagta
     6541 gctgtgcaga cacaatggtt taaacaatat ctttaattgt cagtatttat acgttaaagg
     6601 tcgttgaaat taacttcctg ttacgatgag ttgtttcaac ggcctgcagg gcatattttc
     6661 acgaaggtca gagtaagcat ggcagagcaa aaacgaccgg tactgacact gaagcggaaa
     6721 acggaagggg agacgcctgt ccgcagccgg aaaaccatca tcaatgtcac cacgccacca
     6781 aagtggaagg tgaaaaagca gaagctggcc gagaaagcag cccgggaagc agagctggcg
     6841 gcaaaaaaag cgcaggccag acaggcgctg tccatttatc tgaacctgcc cacactggat
     6901 gaggccgtga ataccctgaa accctggtgg ccgggattat ttgacggtga cacgccccgg
     6961 cttctggcct gcggtatccg ggacgtgtta ctggaagatg tggcgcagcg gaacatcccg
     7021 ctctcgcata aaaaactgcg cagggcgctg aaggccatca cccgttcaga aagctatctg
     7081 tgtgccatga aagccggtgc ctgccggtat gacacggaag ggtatgtaac ggagcatatt
     7141 tctcaggagg aggaagcgta tgcggcagcg cgtctggata aaatccgccg ccagaaccgg
     7201 ataaaggcag aacttcaggc cgtgcttgat gagaaataaa aaaagcctcc ctcagagagg
     7261 ggaggcaggg aaataaggct taaaaggaaa taacttccgt acccgaaaga aaactagtcc
     7321 actgaattat ttatcacaat aatactgaca actaaagggg gacaggaaaa tgaatggatt
     7381 cagaaacagt tcacgaaacg gccaggtctg gcgctggcag cgtaccggac acagggctgt
     7441 taatctggaa atccgtggat tctggatgga ggctgctgag gcgtggtgtc aggccgccgg
     7501 aattgctccc cgggcggact ggcgacgatt tgcccggcta cgcgcagaac gatgccggaa
     7561 atatggacgg aaggaataaa taaagaggct ggcatatttg agattaaatt cataaaactc
     7621 accagcgatg aatcattgcg gatggtgttt caggaatata aaaaaacagc ctccgtttct
     7681 ccagggtaag gaggctgaat gatgcatgag atttgctttt gtattatgtg agacaacctg
     7741 gcactctgta tttccgttct tcagaacgga ttactcatgg cgcaccggat aaccgaccgg
     7801 cttatagtga cacaaggatg tttatgaatt aagtggtggt ttattgattt gtgatatcac
     7861 tcacggaact ccgggcagcc tgactggtgg cagacaatat tttatatccc gataatgaaa
     7921 acttaaccag atcggtagat acacgacgtc ggggttgcag tttattctgt tttattatag
     7981 tgaatatata atgaaaaaca tgatgagaaa tgaagcatta cgcattgcgc tcattgatta
     8041 tgaaaactgt tgcaatctga gaaatatatc gcttgctgat tataccgatt tgattttatt
     8101 tgctggtcca cttcagcaaa cagtggtctt acctgctgat acctggccag ataatatcag
     8161 agtgagtatc agacagatag ataacatttc gaagaataat gttgactttc accttgttat
     8221 ggagctcggc aggatgacgt gctgcagtgc cggaaacaac acctggcata ttatttcaaa
     8281 cgataaaggc tatgatggaa taattcatac tctgcaaaaa cggggttttc gttgcgaacg
     8341 tattgcgcca gagcacgctg agagtcattt gccggtatct cacctgattg tccctgaggg
     8401 ggatgttatt atccggtggg ctaacagaat gcagcaggca tccggaagtg atgtgcggaa
     8461 tttaccggtg tcagtggagg ggtttaataa ttatttaaaa tcgcatatgc gaggagaatg
     8521 gcataaagaa cgggctgtga gtatacgtag tgagttggtt cgtcgtgggg taattaaaat
     8581 ccaggctgat aaaattgtct ggttaacgag gcggtgaccc cttatcacaa cagaattatc
     8641 tgttgcagat ctggtttgtc gtatttctga taacgttcag gaaattcatg ggataaatat
     8701 tgcgagtctt acttcgggtg aaaactacat tcgttcatct ggactcatat gcaattaagg
     8761 acataataat gaatttaatt attttcattg tggttgttat tattatagct gttttgtctg
     8821 tccggataat attccggagg agtaaaaact gtcctcccgc actgatacgg ttacatgcag
     8881 caggcatacg ccccggtgaa gctgagcgaa tactggtcag cggtgaatac tggcagcgtc
     8941 agaaaacgtt gctgacagaa agagaggtca gtttcatgaa aggactgttc agaattgtgg
     9001 atatgaagcg gtggtatctg tgtccgcagg tacgggtcgc agatatcgtc cagctgaacg
     9061 ggaatatccg gccacgatca cgccagtggt ggcagttatt caggatggtg tctcagtggc
     9121 atgttgatgt ggttatcgtt gagcggcgtt cgttcagtat tgttgcagca gtagagctgg
     9181 atgatgccag ccatttacga cctgaacgca gacgccggga tattcttctt gaagaggttc
     9241 tgaggcaggc tggtattccg ttgctcagaa gccacgatgc cagaaaactg ctgcagatga
     9301 ccggagaatg gctgaataca acaggggctg cccagcagtc accggaacat cgtagctgac
     9361 gccttcgcgt tgctcagttg tccaaccccg gaaacgggaa aaagcaagtt ttccccgctc
     9421 ccggcgtttc cataactgaa aaccatacta tttcacagtt taaatcacat taaacgacag
     9481 taatccccgt tgatttgtgc gccaacacag atcttcgtca caattctcaa gtcgctgatt
     9541 tcaaaaaact gtagtatcct ctgcgaaacg attcctgttt gagtattgag gaggcgagat
     9601 gtcgcagaca gaaaatgcag tgacttcctc atcaggtaca aagcgagcat acagaaaagg
     9661 taatcctctg acgcttgcag aacgtcagca ggcatccctg gcaagaaaac gagctacaca
     9721 taaagaactc agagtgttca ttcctgctgc gttaaaagcg caattgcagg tgatgtgtga
     9781 agctgagggc gttacccagg ctgagatgat tgccgaactg ataaagcaga agagtgcttt
     9841 tagctaacta aaatgtagcg acatcacatt cttgcctgtt tctggtgtgc gtgatagatt
     9901 gctgatcgtt taaggaattt tgtggctggc cacgccgtaa ggtggcaggg aactggttct
     9961 gatgtggatt tacaggagcc agaaaagtga aaaccccgat aatcttcttc aagtttggcg
    10021 acatgaaaga ttaccggggc catctaaaaa ccgtatagcc aacaattcag ctatgcgggg
    10081 agtatagtta tatgcccgga aaagttcaag acttctttct gtgctcactc cttctgcgca
    10141 ttgtaagtgc aggatggtgt gactgatctt caacaaacgt attaccgcca ggtaaagaac
    10201 ccgaatccgg tatttacacc ccgtaaaggt gccggaacgc tgaagttctg cgaaaaactg
    10261 atggaaaagg cggtgggctt cacctcccgt tttgatttcg ctattcatgt ggcgcatgcc
    10321 cgttcccgtg gtttgcgtcg gcgcatgcca ccggtgctgc gtcgacgggc tattgatgcg
    10381 ctcctgcagg ggctgtgttt ccactatgac ccgctggcca accgcgtcca gtgctccatc
    10441 accacgctgg ccattgagtg cggactggcg acggagtctg ctgccggaaa actctccatt
    10501 acccgtgcca cccgtgccct gacgttcctg tcagagctgg gactgattac ctaccagacg
    10561 gaatatgacc cgcttatcgg gtgctacatt ccgaccgaca tcacgttcac actggctctg
    10621 tttgctgccc ttgatgtgtc tgaggatgca gtggcagctg cgcgccgcag tcgtgttgaa
    10681 tgggaaaaca aacagcgcaa aaagcagggg ctggataccc tgggtatgga tgagctgata
    10741 gcgaaagcct ggcgttttgt gcgtgagcgt ttccgcagtt accagacaga acttaagtcc
    10801 cgtggaataa aacgtgcccg tgcgcatcgt gatgcgaaca gggaacgtca ggatatcgtc
    10861 accctggtga aacggcagct gacgcgtgaa atctcggaag ggcgcttcac tgccagtcgt
    10921 gaggcggtaa aacgtgaagt ggagcgtcgt gtgaaggagc gcatgattct gtcacgtaac
    10981 cgcaattaca gccggctggc caccgcttcc ccctgaaagt gacctcctca gaataatccg
    11041 gcctgcgccg gaggcatccg cacgtctgaa gcccgccagt gcacaaaaaa acagcgccac
    11101 atacaaaaaa caacctcatc atccaccttc aggtgcatcc ggtcccccct gttttcgata
    11161 caaaatacgc ctcactgacg gggaattttg cttatccaca ttaaactgca agggacttcc
    11221 cgataaggtt acaaccgttc atctcataaa gcgccagccg tcagcgttac agggtgcaat
    11281 gtatctttta aacacctgtt tatatctcct ttaaactact taattacatt catttaaaaa
    11341 gaaaacctat tcactgcctg tcctgtggac agacagatat gcacctccca ccgcaagcgg
    11401 cgggccccga ccagagctac tttagttaca acacacaaac agaaccacca ggaaaaaccc
    11461 cggtcccacg cagaactgaa gccacaaagc ccctccccca taactgaaaa gcggccccgc
    11521 cccggccctt cgggccggaa cagagtcgct ttcaattatg aatgttgtaa ctacatcatc
    11581 atcgctgtca gtcttctggc tggaagtatt gagtacacgc tcgtaagcgg ccctgacggc
    11641 ccgctaacgc ggagatacgc cccgactgcg ggtaaaccct tgtcgggacc actccgaccg
    11701 cgcacagaag ctctatcatg gctgaaagca ggtatggtct ggcagggctg ggggtgggta
    11761 aggtgaaatc tatcaatcag taccggctta cgccgggctt cggcggtttt actccggtat
    11821 catatgaaac aacagaatgc cgccttacat gccgctgacg cggcatctgt caacttaagt
    11881 caaaacaaaa aaccaacaaa tttagtcgtt aaaaaatcta aatcctgtaa cggtgtcacg
    11941 tttttagtta gtttgttagt ttatttgttt gttgtgtata atgaatgcac aatcaccaga
    12001 acaggagcag gtttatgcgt ccagcaatat ttgaaccaca gcagattatt gaggcaggtc
    12061 tggcattaca ggccgaaggg cggaatatca ccggattcgc gctacgtaac cgcgttggtg
    12121 gtggtaatcc cgggcggctt aagcaggtgt gggatgaata tatctcgtcg cagacggaag
    12181 tcaccaccgg gccggtggcc gagctgcctg ttgaagtggc cgaagaggtt aaggccgtat
    12241 cgtcagcact ggccgaacgt atcagccagt tagctaccga gttaaatgat aaagccgtca
    12301 gagccgccga acggcgggta gccgaagtga cccgcgcggc gggtgaacag acgacacagg
    12361 ccgagcgcga gctggccgac gccgcacaga ccgttgacga tttggaagaa acactggata
    12421 ccctgcgtga agaacacagc gccacactgg cggcgctgga cgacagccgg aggaaagagc
    12481 aggctcaggc cgtcgaactg gctcaactac gggaacgcct ggccgcgaca gagagactgc
    12541 tgggggaatc agagaaggca ggaaaagagg ccgcagagca gcaccggcag caacaggcag
    12601 acctgcaaaa acggcttgat gcagccggga ataaactggc cgaagccact acgcgtcatg
    12661 cgactgacct gcagggtgtc agggagcagt acgccggaga ggttaaggag cttaaggcgc
    12721 aacatgcccg gaacgaaagc gacctgcaaa ggcggcttga tgctgccgag aaaaaactgg
    12781 ccgaggccat tacgaaccat gctattgaga ttaagggtat caaagagcag cacactggcg
    12841 aaattaaaga gcttaaggca caacatgccc ggagcgaaaa agaactgcaa aataggcttg
    12901 atactgaact aaaagcgcgt caggcagcgc tggataaagc actcagagat gcaacagatg
    12961 cgcgggaagc aaaggcgact ttgactggtg aacttcagtc tctgaaaacg cataacgacc
    13021 agttagcagc gttgctggcc ggaaaaaaat aaatctgcag cggtaaaacc aaaaattcga
    13081 acaaccgtat tggcttacgc cggttttact cctgtgccat atgcaacaac ggagtgccgc
    13141 cttccatgcc gctggcgcgg catatcctgg tgacgatatc tgaatcgtta tatactgtgt
    13201 atatacgtag taataacgag gtgataaatg gcacaggtta atatgagttt aagaatcgac
    13261 gctgaactga aggatgcttt tatggccgct gcaaaaagca tggaccgtaa tggctcccag
    13321 ttaatccggg attttatgcg ccagaccgtt gaacggcagc ataatacctg gttccgtgac
    13381 caggttgcgg caggacgtca gcaactcaag cacggcgatg tgcttcctca tgacatggtc
    13441 gaatcctccg ctgctgcatg gcgggatgaa atgagcagga aggtcgccgg taaatgatgg
    13501 agatattctg gaccatgctg gccagccagg acaggaagcg cattcgtgag tacattgcgg
    13561 agcagaacct gacggccgcc attgaactgg acgaacggat tggttattca gccagcagcc
    13621 tggccgggca gccgtataaa ggtcgcaatg gtcgtgtgga aggtacccgg gaactggtta
    13681 ttcacccgca ttttgttctg gtttatgagg tcgatagcca gtggggaaaa gtgtatattc
    13741 tgcgagtgtt gcatacagca cagaagtggc cataaagaag agtatatttt taccttactg
    13801 ggggctgatt atctgttgtt actgttgtgg catacataga aaaacagttg ggtagttaaa
    13861 atagcataaa ggtttgtgaa atgattggaa aatctatatc tcgtttatgc caaaatgaac
    13921 gcaaaaacag cattgcacaa tcttgcagta ataatcgagt ataaaaggaa acactatctg
    13981 ataagtgttt taactaaaga taagagttct aatagtacag gtactaaaga taattaatat
    14041 gttaataaaa aataaatcag aaattcattt gggcccgcat tttatgctta attatgaact
    14101 cctacaacca ggagatatta ttttggagag aggttatgct tggtattcag aaaaaatagc
    14161 taaacacacc aatagtcgtt actctcatgc aatgatatac gtaggtggga caataattga
    14221 ggcaactagg ggaggtgggg tgtatagccg aattccaaac cgttctactg tacgtgatat
    14281 tagcgacttt aaggtactaa ggcttaaaga gtttccggga aaggatatct tacaagtaat
    14341 atgtgaccat gctcgtagtc tctgtggtag ccagtattca gtaagccagg ctctcaaggt
    14401 taaagggccg gattttttaa gaaaatttgc caacgacagt agaaaacaat tttgttcacg
    14461 gcttgtggct caatgttacc agaaggctgg aatacaactg acagagaata taaatttctg
    14521 ttcatcgata ggaatttaaa tcccaaaaag attaaaaaaa cacccaaaaa cggatgttta
    14581 ttcaacactg cctgcgtgcc atgagtgcac cgccatcaat taaatggatc ttatcgagtg
    14641 atactgtata tcactgatgg tttttttact ccattcccct gaaaatggac gcaacttccc
    14701 cgatgcggct ctcagccgca cacccgctgt tgcgccagtg gctcatgcat caggaccagc
    14761 tctgtcagac ggtagccccg cttcagcccc gtaaaacgca tctgacatcc gcacaggatg
    14821 cactttagcg ggtcaacctt cagtaaccgc tgatacatcc ctctccacct gatttgcatc
    14881 gccgtctttc tcaccgtctc cgttatgacg tacacaacat cttccagtaa ccggcgcttc
    14941 accggactca ggaaaccgta gtacctcacc atacggaacc ccttatccgc cacatgccag
    15001 gagaaccttt ccataaactc atcaccactc atcaccaggt attcttcccg ttttgtccgg
    15061 tgactgttgt aacgcagacc aatttcatcc tgaccagcat agtgctccag acggctcatc
    15121 ggcaccggcg gttttttcag gtaagagccg aagtacaccg ccacatgtgt ggcgttatcc
    15181 atcacccggg atacgttgac attccagccc cgccggtaat gactgtccag gaagcgattc
    15241 cagtcccgtc tgctgcttcc ttcaactgcc agcgcatccg gtatcaccag ctccgggtat
    15301 tttcgtgaca gtaaccacgt tatcctgtaa cgccacatgc tcatcacctt acgggcgtaa
    15361 aaatgaaggt ttttccaggt gtggtctggt gtcacgccgc cggccgttgt cgacaggtga
    15421 atgtgcggat gccactgctg gtcacgtccc catgtgtgga tgaccgtgaa tatccccggc
    15481 accacatctg cctggcggca gatttcctgt atcacatccg cagcaatgcg gctcatctct
    15541 gccagtaacc accggttgtg gaacaccagg gaccagtact ggcagggaag tgtgaacaca
    15601 atatgctgcc acggacagtc gggaaccaga ctcagcagat actgtatcca ctgtgcgcca
    15661 gccttcactc cgcagtgcgg gcaggagcga cttttacacc ggaagcagac cttttttgtg
    15721 tggcaacagt ccggagatga acagcaccac tgtgtgtacc ccatcagcgt ggttccgcac
    15781 gccatgattt ttgtcaccga ctcaatgact accggacgca ctgaaccttc cggttgtttc
    15841 tccagccagt taagccagcg gtttccctgc tgaaaaatgt cggcaaaacg gggaagcatc
    15901 agaagggcgg ggggactccg tccggccagt gaaccgtgcc acactccggg cagtacatgc
    15961 cgccggcgct gataccggca agaatggtcg caaactcccg ctccgtgcag cgggctattt
    16021 ccggataccc ttcgtcatcg acacgaacaa accagaagac cagctttttg tttccggcat
    16081 ccacaaagaa gggaatattc aggtctgcgc agcactcaac ggcatcgtca aaattatcaa
    16141 agcgcagagc ttctgcgtct tcttcgtcaa agaagtcatc gtcgtgaagc ttcacgacat
    16201 agcggggaag tttgcttctt tgaggggcag gttttcgttt acggggttta gctgaacggg
    16261 gcatataact acctgaaaga caatgatatt gtctgttttt ataacggtaa tttcaggcca
    16321 tgacaagccg cagccgccag gctgcctact cgattcttat ttaggaatga tagatgtaaa
    16381 aaatattaaa ctcaaagaca tttgagtttt tcaactacag ttggatgctt tacagaacat
    16441 aagtattgtg aagattagag tgggcagaat atattctgcc cctctttgtc atttttttaa
    16501 catagacaga ttgcgtctgg tttgttcttc gaaatcatct ttcggattga tgctcgctga
    16561 tttgttttct gaagatgcct ctatgatgtt ttttaacaca cttatatcaa ttgatgtcga
    16621 tgtgtatcct gttgtttgtt ctataagttt caccagattt tctgcagtga ttttctcact
    16681 gaaaaaaccg ctgattagtg ttaacagtcg ggggtcaaca gaataaagag ctgctccgca
    16741 aataagggat tgtctgtaga aatctccccg catttgtact gggagagagt ctattgtttg
    16801 ttgtgtcagg ttgtcctgcg gataaagtgc cgggtgcaga tatgccacaa ctttctttct
    16861 ttttttggat cactgctttc cattacgcat gcaccatggc ttttaccaat gcaaactgag
    16921 gttcatctac cttgttcact ttcagattcg ggaagtattg ctttatgtga gggtaaataa
    16981 gctcggctcc cccgcctgtg aggtagattc ggttcattcc tgagaatgtt gaaatttcct
    17041 gcctgatgct ttctgccaga gatttcacct cactgtctat tacatgagta atatttttta
    17101 tttcagctac atcatttatt aatgtctgga gatattcatt atcatgcctg ttttttatta
    17161 actcatcagc aataaaataa ttgcagggtg tggaggcttt tgttagtgca ttcatgacgg
    17221 tatgagttat gcgggatgtc ccgatttctg aatagccacg gatttcagaa atgccttcaa
    17281 atgcaccgag aattaaacca caatccagag tggttcctcc aagatcgata atcaggctgc
    17341 gttcaagttt gtttacttta tcttttttta gtgattcaaa tgcagccggc aaagattcag
    17401 gcattacatt aactttttta attttaaatg tttccccttt attcagagat atctcacgga
    17461 gaacatttgc cttttttctt tctattcttt cttcatttgg ttgattgtca ttatcaaaga
    17521 attcggttac aggtaatgtt actgtcagtt ctacatcctg tggctgaagg ccggatgtca
    17581 gcagtgcatg atgtatggca agcagattgg tggtgctgta ttgataactg acatgtgttg
    17641 tacctatgac agcagtgctg cccaaatcat aggtgtattt ttttccatcc acgacgtaat
    17701 tgtaaacttt gccggcatta aacagtcccg ggttccagcc ttctttaaag ctgtttcctg
    17761 acagactggt tcttacctcg ccatcttcaa gccaggccag ttttacgtta gttgaaccat
    17821 catcgcatga tacttttagc attctttttg cctcgttttg agtttgtgtt tgtctcaaat
    17881 tgagcatctt ttatgtcgat gcatcttttt gagtttttgt tatttcttat tgtgtctcat
    17941 tttgggttaa tgtcaagtaa tgtgactctt ttagagtctc cttgtgttct tgctgtaact
    18001 caatttgagt cgttgctggt ttctcatttt gcgtcacaaa ctgagacgga aactacttaa
    18061 ccaggccgtg ccggcacgtt aaccgggctg catccgatgc aagtgtgtcg ctgtcgccgg
    18121 cctcctcacc cggtcacgtt tcgtcgttcc tcctccacgc gctgcggctt cggggccgca
    18181 cctgcattcg tatgcggtcg cccggttaca ggtgcggcac ggcctgatgg aggccgcatg
    18241 tgagaggaga attcccatgc caaactggtg ctcaaatcgt atgcattttt ctggtgaacc
    18301 ggcacagatt gctgagatta aacgactggc cagcggtgca gtcacaccgt tttatcgccg
    18361 cgccacaaat gaaggtaccc gcgaacaggc tgaagccgtt gccgcacagt accggaatgt
    18421 ctttatcgag gatgatcagg gaacgcattt tcgcctggtt gtccgcaata acagtggcct
    18481 ggtctggcgt acctggaatt ttgaggacgg tgccgggtac tggatgaacc atgtcatccg
    18541 gaatttcggc attcttaagt aagagaggtg ccggacggga acgcgtccgg cagacataca
    18601 accatcccgg tcaaaggaga aatatatgtc agttacagag tctaagacaa aaacggagcg
    18661 taaatccagc cgtaaacctg caaaaacgca ggaaacagtc ctgtctgccc tgctggagca
    18721 gacggaggaa gtgagcgtgt cgctggcctc gctgattaaa tcaccgctga atgtgcgcac
    18781 ggtgccgtat tccgcagagt ccgtcagcga actggcggag tccattaagg gcgttggcct
    18841 gttgcagaat ctggttgttc atgccctgcc tggtgaccgc cacggtgtcg ccgcaggtgg
    18901 tcgccgactg gcagcactca acatgctggc agagcgtgac atcattcagg ctgactggcc
    18961 tgtccgtgtg aaggtcattc cgcaggagct ggcgactgcc gcatcgatga ccgagaacgg
    19021 tcatcgtcgg gatatgcacc ctgccgaaca gattgccgga ttccgcgcaa tggcgcagga
    19081 aggcaaaaca cctgcacaca tcggtgattt gctgggctat tcaccccgcc acgttcagcg
    19141 aatgctgaaa ctggctgacc ttgcgcctgt catcctcgat gcgctggcag aagaccgcat
    19201 caccaccgaa cactgtcagg cgctggagaa cgacaccgcg cgtcaggtgc aggtgtttga
    19261 agccgcctgt cagtcgggat ggggcggtaa accggaagta cagaccattc gtcgtctggt
    19321 gaccgaaagt gaagtggcgg tggcggggaa cagtaaattc cgcttcgtgg gggctgatgc
    19381 cttctcgcca gacgaactgc gcaccgattt gttcagcgac gacgaaggtg gctatgtgga
    19441 ctgcgtggcg ctcgatgccg ccctgctgga aaaactccag gctgtcgcag agttccttcg
    19501 ggaaccggct ttgtgatgaa aatattgata attttgattt tctgaataca cctgaaaaga
    19561 cgttaatcct tgatggagtc aggctgtgtg aaaagaaaat gacatcaaaa gttatcaggt
    19621 taattaaaca ggccagaaaa tatcataaac gcctggttgt ggttgcagac tcatgtgagt
    19681 ctgagtttat cgaactgttg tttgatggtg ttattgcatt gtccttcaat tctgacagag
    19741 aaaggagttg caatgtttat acgccatcaa gtcatcgtaa tacagataat atttatgcca
    19801 ggtaaatgtg taaacaaaaa cagaggaggg tgta
//
