LOCUS       NZ_CP122594             9359 bp    DNA     circular CON 08-JUN-2024
DEFINITION  Escherichia coli strain ETEC6335 plasmid unnamed6, complete
            sequence.
ACCESSION   NZ_CP122594
VERSION     NZ_CP122594.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32549676
            Assembly: GCF_030013005.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9359)
  AUTHORS   Vereecke,N., Van Hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Virotyping and genetic antimicrobial susceptibility testing of
            porcine ETEC/STEC strains and associated plasmid types
  JOURNAL   Front Microbiol 14, 1139312 (2023)
   PUBMED   37143544
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9359)
  AUTHORS   Vereecke,N., Van hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2023) Department of Pathobiology, Pharmacology and
            Zoological Medicine, Universiteit Gent, Salisburylaan 133, Merelbeke
            9820, Belgium
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Trycycler; minimap2; medaka v. v0.5.3; v2.20;
            v1.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 260x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030013005.1-RS_2024_06_08
            Annotation Date                   :: 06/08/2024 05:27:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,662
            CDSs (total)                      :: 5,534
            Genes (coding)                    :: 5,336
            CDSs (with protein)               :: 5,336
            Genes (RNA)                       :: 128
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 198
            CDSs (without protein)            :: 198
            Pseudo Genes (ambiguous residues) :: 0 of 198
            Pseudo Genes (frameshifted)       :: 83 of 198
            Pseudo Genes (incomplete)         :: 121 of 198
            Pseudo Genes (internal stop)      :: 29 of 198
            Pseudo Genes (multiple problems)  :: 27 of 198
            Pseudo Genes (short protein)      :: 1 of 198
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP122594.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ETEC6335"
                     /isolation_source="feces"
                     /host="Sus scrofa"
                     /db_xref="taxon:562"
                     /plasmid="unnamed6"
                     /geo_loc_name="Netherlands"
                     /collection_date="2021"
                     /collected_by="IMM_DI04"
     gene            join(9047..9359,1..11)
                     /locus_tag="QDY30_RS27065"
                     /old_locus_tag="QDY30_27065"
     CDS             join(9047..9359,1..11)
                     /locus_tag="QDY30_RS27065"
                     /old_locus_tag="QDY30_27065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_282867006.1"
                     /translation="MLTIRVNDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            220..>651
                     /locus_tag="QDY30_RS27070"
                     /old_locus_tag="QDY30_27070"
                     /pseudo=""
     CDS             220..>651
                     /locus_tag="QDY30_RS27070"
                     /old_locus_tag="QDY30_27070"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            689..1207
                     /locus_tag="QDY30_RS27075"
                     /old_locus_tag="QDY30_27075"
     CDS             689..1207
                     /locus_tag="QDY30_RS27075"
                     /old_locus_tag="QDY30_27075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            1214..1447
                     /locus_tag="QDY30_RS27080"
                     /old_locus_tag="QDY30_27080"
     CDS             1214..1447
                     /locus_tag="QDY30_RS27080"
                     /old_locus_tag="QDY30_27080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1765..2184)
                     /locus_tag="QDY30_RS27085"
                     /old_locus_tag="QDY30_27085"
     CDS             complement(1765..2184)
                     /locus_tag="QDY30_RS27085"
                     /old_locus_tag="QDY30_27085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(2265..2702)
                     /locus_tag="QDY30_RS27090"
                     /old_locus_tag="QDY30_27090"
     CDS             complement(2265..2702)
                     /locus_tag="QDY30_RS27090"
                     /old_locus_tag="QDY30_27090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            2962..>3477
                     /gene="cea"
                     /locus_tag="QDY30_RS27095"
                     /old_locus_tag="QDY30_27095"
                     /pseudo=""
     CDS             2962..>3477
                     /gene="cea"
                     /locus_tag="QDY30_RS27095"
                     /old_locus_tag="QDY30_27095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /name="cea"
     gene            complement(3509..5080)
                     /locus_tag="QDY30_RS27100"
                     /old_locus_tag="QDY30_27100"
     CDS             complement(3509..5080)
                     /locus_tag="QDY30_RS27100"
                     /old_locus_tag="QDY30_27100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="WP_000381443.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAKYNRLLSQRVAAYASEIN
                     RLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLDEQYDPVLPSAL
                     RQSSARKPLPASLPRETRVIRPEEECCPACGGDLSPLGCDVSEQLELISSAFKVIETQR
                     PKLACCRCDHIVQATVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQGVEL
                     SRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTARLWVYV
                     RDDRNAGSQMPPAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESGRITEAA
                     CMAHARRKIHDVHARVPTDITTEALQRIGELYVIEAEVRGCSAEQRLAARKARAAPLMQ
                     SLYDWIQEQMKTLSRHSDTAKAFAYLLKQWEALNVYCSNGWVEIDNNIAENALRGVAVG
                     RKNWLFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPANRVRDLLPWK
                     VDLTSQ"
                     /name="IS66 family transposase"
     gene            complement(5100..5447)
                     /gene="tnpB"
                     /locus_tag="QDY30_RS27105"
                     /old_locus_tag="QDY30_27105"
     CDS             complement(5100..5447)
                     /gene="tnpB"
                     /locus_tag="QDY30_RS27105"
                     /old_locus_tag="QDY30_27105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="WP_000624618.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLFI
                     FRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDWKH
                     PKRTERAGIRI"
                     /name="tnpB"
     gene            complement(5447..6097)
                     /gene="tnpA"
                     /locus_tag="QDY30_RS27110"
                     /old_locus_tag="QDY30_27110"
     CDS             complement(5447..6097)
                     /gene="tnpA"
                     /locus_tag="QDY30_RS27110"
                     /old_locus_tag="QDY30_27110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001690050.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="WP_000993956.1"
                     /translation="MNARKAVLADNPELILRVLQLRFDESLSYPRISAQTGVSKTAIFS
                     LVRRFHQVFTDWPLSGEYSCGQLARALFPGRYPSAPTVTQPVKAEKPRRNRFSPEFKWR
                     LVQQTLLPGACVAQIARENGINDNLLFNWRHLWRNGGLQPPGEHETSLLPVTLTPEPDN
                     KIPAPVQIPEQINTLPDSLCCELVLPAGTLRLKGELTPALLQTLIREMKGSSH"
                     /name="tnpA"
     gene            <6184..7242
                     /gene="cea"
                     /locus_tag="QDY30_RS27115"
                     /old_locus_tag="QDY30_27115"
                     /pseudo=""
     CDS             <6184..7242
                     /gene="cea"
                     /locus_tag="QDY30_RS27115"
                     /old_locus_tag="QDY30_27115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /name="cea"
     gene            complement(7239..7580)
                     /locus_tag="QDY30_RS27120"
                     /old_locus_tag="QDY30_27120"
     CDS             complement(7239..7580)
                     /locus_tag="QDY30_RS27120"
                     /old_locus_tag="QDY30_27120"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_000058759.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNSEGYYFLVSDKMLYAIVI
                     STILCPYSKYAIEHIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            7628..7765
                     /locus_tag="QDY30_RS27125"
                     /old_locus_tag="QDY30_27125"
     CDS             7628..7765
                     /locus_tag="QDY30_RS27125"
                     /old_locus_tag="QDY30_27125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(7964..8122)
                     /locus_tag="QDY30_RS28375"
     CDS             complement(7964..8122)
                     /locus_tag="QDY30_RS28375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(8792..8983)
                     /locus_tag="QDY30_RS27130"
                     /old_locus_tag="QDY30_27130"
     CDS             complement(8792..8983)
                     /locus_tag="QDY30_RS27130"
                     /old_locus_tag="QDY30_27130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          8620..8707
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(8051..8606)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.878"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2..1551
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9018..9358
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.491"
                     /coverage="97.0"
                     /evalue="1.98e-65"
ORIGIN
        1 atgatagtta aatttcatgc caggggaaaa ggtggtggca gtggtccggt tgattacctg
       61 ctggggaggg agcgtaaccg ggaaggggca acggtgcttc agggtaatcc ggaagaagtc
      121 cgggaactca tcgatgccac gccatttgcg aagaaataca cgtccggtgt tctgtcgttc
      181 gcggagaagg agctgccgcc gggaggacgt gaaaaagtga tggcgagctt tgagcgtgta
      241 ctgatgcccg gtctcgaaaa aaatcagtac agcatcctgt gggtggagca ccaggacaag
      301 ggacggcttg agctgaattt tgtcattccg aacatggagc tacagaccgg aaaacgcctc
      361 cagccgtact acgaccgcgc agacaggcct agaattgatg cctggcagac gctggtgaat
      421 caccattacg ggctgcatga cccgaacgcc ccggagaacc gcaggacgct gacactccct
      481 gataacctgc ctgaaacgaa acaggcgctt gctgagggcg tcacgcgagg tatagatgca
      541 ctttaccatg ccggagagat aaaaggccgt caggatgtga ttcaggcgct cactgaggcg
      601 gggctggaag tggtcagggt gacgcgaagc agtatcagca ttgcagatcc gaacggcggg
      661 aagaatatca ggctgaaagg agcattttat gagcaatctt ttgcagacgg gcgcggagtt
      721 cgagaaaaag ctgaaagaga gagccgaatc tacagagaaa atgctgaaca acgagttcag
      781 gaggctcggc gaatctgtaa gcgaggctgt gacatcaaac gagacgaaaa tcagagacgc
      841 tatagccctg ttcacagcct cgacagagga atcgctggaa aaacaccggg aaggggtgaa
      901 agaggcgatg atgcagcaca ggagggacgt gttaaagctg gcagggaata cgggcatgat
      961 gttactgggg atagtctttc tcctgtttac cgcgagtggc gggacgctct ggtatcttgg
     1021 agggaggata caggcgaacc tggaagaaat caggaagcag gaagagacat tgcagaaact
     1081 gaacgcgaag acatggggcg tggagtttgt gcaggacggg aacaggaaat tccttgtcct
     1141 tccgtacggg aaatcagcgg aggtgattcc ctttcagggg aaagagtggg tacatctgaa
     1201 ggagtgacac agagtgacag agctggaaac acatttgctg aacgccttag agcagctgca
     1261 acaggactat atgcagcggc tgagcgaatg ggagagcgcc ttcgtggaat tgcagaagat
     1321 gttttcgctt acgcaacggg acaacgcgat gctgaacgag cgggtcatgc agttgagtca
     1381 gcaggtgcag cacttgagcg agcagacaga acgcttgagc cagttataca gcgagaactg
     1441 gagataagag aggaacggct gatacaggag cgcgaacatg tgttatccct ggaacgggag
     1501 cgtcagccgg aaatacagga acgcacgctg gatggccctt cgctgggatg gtgaaaccat
     1561 gaaaaatggc agcttcagtg gattaagtgg gggtaatgtg gcctgtaccc tctggttgca
     1621 taggtattca tacggttaaa atttatcagg cgcgatcgcg gcagtttttc gggtggtttg
     1681 ttgccatttt tacctgtctg ctgccgtgat cgcgctgaac gcgttttagc ggtgcgtaca
     1741 attaagggat tatggtaaat ccacttactg tctgccctcg tagccatcga gataaaccgc
     1801 agaaatcgtg tcagccagca gcctggattt tttcggggta aggtctttgc tgcggtaatc
     1861 agtacccgca aattgagttg tgccccggag tgagtttaaa aattcctggc tgtgaaactg
     1921 ctgaatgcgt tgttgcacat cactccgtga ttttcctgtc gctttatctt tagctcccat
     1981 atcccagaac tgccgaaaaa aaggcaccat cattcgtgtt gtgtcggctt tttgggtgga
     2041 aaagttggga tcaaaaccgt catcagaagc atatacgtaa tgccgggtat gttgttccac
     2101 gctgcccttt ggggtacagg ctgtgagagc tactgttgct gttatcatgg cgataagaca
     2161 aagtttgttt ccggtacgtc tcatgagtgt ctccctacct taaagtattt attctcggtg
     2221 agttattatc cgaagactat tttatcatca gtctctgaca gcgttcagcg accgaacaac
     2281 ctggaccaga atccccgttt cttttccagt tccagtgcct gcctgagctg ggctatctca
     2341 ttttgtagct gttcccgttc tgcttcctgg cgtctgcgat ccatatcctg tgcctgttta
     2401 tcctcaagca tcagcgtcag gcattgtttc agctcattca gctcccggag aatgcgttct
     2461 gtctgctgat catgtggatt ttctgcatgt ccctcactgt gcctttctgg tgtctcattc
     2521 tgctttaatt cgccgtatgc ccggatcagt tcactggttt caaactcccg tcgtccgtcc
     2581 ctgccggtac ggtaactgac aaggcctgcg cgcatatcac ggtaaaactg tgaacgcgat
     2641 ctgcctgtca tttttagtgc gtcccggagt gtgtgccatg ccataaagtg acagtgtccc
     2701 atagatgtct catctcatag tttcagtaaa acataatgag gtctgagaac ggtaatgttt
     2761 gtgctggttt ttgtggcatc gggcgagaat agcgcgtggt gtgaaagact gtttttttga
     2821 tcgttttcac aaaaatggaa gtccacagtc ttgacaggga aaatgcagcg gcgtagcttt
     2881 tatgctgtat ataaaaccag tggttatatg tacagtattt atttttaact tattgtttta
     2941 aaagtcaaag aggattttat aatggaaacc gcggtagcgt actataaaga tggtgttcct
     3001 tatgatgata agggacaggt aattattact cttttgaatg gtactcctga cgggagtggc
     3061 tctggcggcg gaggtggaaa aggaggcagt aaaagtgaaa gttctgcagc tattcatgca
     3121 actgctaaat ggtctactgc tcaattaaag aaaacacagg cagagcaggc tgcccgggca
     3181 aaagctgcag cggaagcaca ggcgaaagca aaggcaaaca gggatgcgct gactcagcgc
     3241 ctgaaggata tcgtgaatga ggctcttcgt cacaatgcct cacgtacgcc ttcagcaaca
     3301 gagcttgctc atgctaataa tgcagctatg caggcggaag cagagcgttt gcgccttgcg
     3361 aaagcagaag aaaaagcccg taaagaagcg gaagcagcag aaaaggcttt tcaggaagca
     3421 gaacaacgac gtaaagagat tgaacgggag aaggctgaaa cagaacgcca gttgaaacgt
     3481 aagcggctcg ccagaaccgt attgatattt actgagaggt cagatcaact ttccagggca
     3541 acagatcgcg tacccggttt gccggccaat cctggatatg ctcaatgacg taacgcagcc
     3601 atttttctgg ttccacattg ttcagacggc atgtgccgat cagcgagtac aacaccgccg
     3661 catgttcgcc accgctgtcg gaacccgcga agagccagtt tttccggcct acggccactc
     3721 cccgtaaggc gttctctgcg atgttgttgt cgatttccac ccagccatta ctgcagtaca
     3781 cgttcagggc ctcccactgt ttcagcaggt atgcgaacgc ttttgccgta tccgagtgac
     3841 gcgacagtgt tttcatctgt tcctgtatcc agtcatacag tgactgcatc agtggagccg
     3901 ctctggcttt tcttgctgcc agacgctgtt ctgctgaaca tccccggact tctgcctcga
     3961 tgacatacag ttcaccgata cgctgcaggg cttccgtggt gatgtcggtt ggcactcttg
     4021 catgcacatc gtggattttt ctccgggcat gggccataca cgcggcttcc gttattctgc
     4081 cggattcgta taacgcccgg taaccaccgt aagcatcggc ctgaagcaca ccgctgtaac
     4141 cggccaggtg attctgtgga tggatacctt tccggtccgg gctgtacgcg aaccagaccg
     4201 ccgggggcat ctgtgaaccg gcgttacggt catcacggac atagacccac agccgggcag
     4261 ttcttgtttt accactgccc ggctcctgga ccggaacggg gatatcatcc gcatggactt
     4321 taccgggcat cagcacatac tggcgcagga cgtcatacag cggctccagc agttcagcaa
     4381 cggcacctgt ccagcgcccc agcgtggcgc ggctcagctc cacgccctgg cgacggtata
     4441 tttctgactg gcggtataac ggcagatggt ctgcatattt cccggtaaca acatgggcca
     4501 gaagccccgc tccggcataa ctgcgtgcaa tgggttttga aggtactgtt gcctgcacga
     4561 tatggtcgca ccggcaacag gccagtttcg gacgttgtgt ttcgataacc ttaaaggcgc
     4621 tgctgataag ctccagttgc tctgacacat cacaccccag aggactgagg tcaccaccac
     4681 aggccgggca gcattcctct tccggccgga tgacccgggt ttcacgggga agtgaggccg
     4741 gtaacggctt acgggctgaa gactggcgca gggcggatgg cagtaccggg tcatattgct
     4801 catccagcgt ttccgccatt tcttcctgaa gtgcgctgat tcgctcctgg gcttcctgta
     4861 tctgccgttc ggttttagcg cgaagttttt ctgagctttt accgaactgc atgcgctgca
     4921 gtttcgcaac cagcgccttc agccggttga tttcggaagc ataagccgcc acccgctgtg
     4981 agagcaggcg gttgtatttc gccatctggc ggatggtgtc ctgttgcgtc tgcaacagtg
     5041 cccgcaggcg ggcattctca tgagcaagtg aggtgtccat atcctcactt tacaacgggt
     5101 tatatgcgga tgccagcgcg ttccgttcgt ttcgggtgct tccagtcgat gccttcgaga
     5161 agcatggaca actgagccgg agtaaggtga acctttccat cgcgggtgac cggccagacg
     5221 aagcggcccc gctccaggcg tttggtgaag aggcacagtc cgtcactgtc agcccacagc
     5281 acttttatct ggtcaccccg gcgtccgcgg aagatgaaca gatgcccgga gaacgggtca
     5341 tccttcagga cgttctgaac ttttgaggcc aggccgttaa agccatttcg catatcggtg
     5401 ataccggcaa ccagccagat acgcgaaccg gcagggagag atatcatcag tggctgctcc
     5461 ctttcatttc gcggataagt gtctgtaata acgccggtgt cagttcacct ttcagcctga
     5521 gagttccggc cggcagaacc agctcgcagc acagactgtc tggcagtgta tttatctgtt
     5581 caggtatctg cactggtgcc gggattttat tatccggctc cggagttaac gtcacgggaa
     5641 gtagcgatgt ttcatgttcg ccgggcggtt gcaggccacc gttacgccag agatgccgcc
     5701 agttaaagag caggttatcg ttaattccat tctctcgagc tatttgtgcg acacaagcac
     5761 cgggtaaaag agtttgctga accagtctcc atttaaactc cggtgaaaat cggttccggc
     5821 ggggtttctc tgctttcaca ggctgagtca cggtcggggc tgaagggtat cgccccggga
     5881 aaagagcccg ggccagttgc ccgcaggaat attcaccgga aagaggccag tcagtgaata
     5941 cctggtgaaa tcgcctcacc agagaaaaaa tggcggtttt gctgacaccg gtctgcgcag
     6001 aaatgcgcgg gtacgacagt gactcgtcaa atctcagctg tagcacacgc aggatcaatt
     6061 ccggattgtc tgccagtacc gcttttcgtg cattcatctt aaatgtccct ttactgcgaa
     6121 aatggacatt agtatcggaa acaggaaagg gaggcgaaag acggtttaaa tgagacggtt
     6181 acgttgaaac tggctgaagc tgaagagaaa cgactggctg cattgagtga agaagctaaa
     6241 gctgttgaga tcgcccaaaa aaaactttct gctgcacaat ctgaagtggt gaaaatggat
     6301 ggagagatta agactctcaa ttctcgttta agctccagta tccatgcccg tgatgcagaa
     6361 atgaaaacgc tcgctggaaa acgaaatgaa ctggctcagg catccgctaa atataaagaa
     6421 ctggatgagc tggtcaaaaa actatcacca agagccaatg atccgcttca gaaccgtcct
     6481 ttttttgaag caaccagacg acgggttggg gccggtaaga ttagagaaga aaaacaaaaa
     6541 caggtaacag catcagaaac acgtattaac cggataaatg ctgatataac tcagatccag
     6601 aaggctattt ctcaggtcag taataatcgt aatgccggta tcgctcgtgt tcatgaagct
     6661 gaagaaaatt tgaaaaaagc acagaataat ctccttaatt cacagattaa ggatgctgtt
     6721 gatgcaacag ttagctttta tcaaacgctg actgaaaaat atggtgaaaa atattcgaaa
     6781 atggcacagg aacttgctga taagtctaaa ggtaagaaaa tcggcaatgt gaatgaagct
     6841 ctcgctgctt ttgaaaaata caaggatgtt ttaaataaga aattcagcaa agccgatcgt
     6901 gatgctattt ttaatgcgtt ggcatcggtg aagtatgatg actgggctaa acatttagat
     6961 cagtttgcca agtacttgaa gattacgggg catgtttctt ttggatatga tgtggtatct
     7021 gatatcctaa aaattaagga tacaggtgac tggaagccac tatttcttac attagagaag
     7081 aaagctgcag atgcaggggt gagttatgtt gttgctttac tttttagctt gcttgctgga
     7141 actacattag gtatttgggg tattgctatt gttacaggaa ttctatgctc ctatattgat
     7201 aagaataaac ttaatactat aaatgaggtg ttagggattt aattattctt tattgatata
     7261 aaaagtccta gcaatccaaa tgggattgct aggaccaaac aaagtagatt atatagcata
     7321 aataggttta attttgctac gggggcgtta tttaggtttt ttcttctttc gaaaaaatct
     7381 ttctttatga agttaaaagc tatgtgttca atagcatatt ttgaatatgg acatagaata
     7441 gtgcttatca ctattgcata tagcatctta tctgacacaa ggaaataata cccttcgctg
     7501 ttttttgtta taaggtatat atatataagt gtgcagtaca ggccaaataa aatatttttt
     7561 atgtagtatc ttaagctcat aaattaaacc tcgccatata ttcttttcat tttataagga
     7621 tcgagttatg aggaaaagat tttttgtggg aatattcgcg ataaacctcc ttgttggatg
     7681 tcaggctaac tatatacgtg atgttcaggg agggaccatc gcaccatcct cctcttctaa
     7741 actgacgggg atcgcggttc agtagaaaag atcaaaggat cttcttgaga tccttttttt
     7801 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccaacggt ggtttgtttg
     7861 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     7921 ccaaatactg tccttctagt gtagccgtag tcgggccact acttcaagaa ctctgtagca
     7981 ccgtttgtgc catcatcgct ctgctaatcc ggttaccagt ggctgctgcc agtggcgtta
     8041 aggcgtgcct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     8101 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     8161 gataccaaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     8221 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     8281 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     8341 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc ctgctacgtg gccttcttcc
     8401 tgttcctggt cttttgctca catgttcttt ccggccttat cccctgattc tgtggataac
     8461 tgtgttaccg tttttgtgtg agtcagtacc gctcgccgca gtcgaacgac cgagcgtagc
     8521 gagtcagtga gcgaggaagc ggaaaagcgc ctggacgtgc attttctcct tacgcatctg
     8581 tgcggcattt cacacccggc atggcgtact tttcatacaa tccgcactga tgccgcatgg
     8641 ttaagccagt atacactccg ctatcgctac gtgactgggt cagggctgcg ccccgacacc
     8701 cgctaaaacc tgctgacgcg ccctgacggg cttgtcagct cccggcatcc gctcacagac
     8761 aagctgtgac cgtctccggg agctgcatgt atcagaggtt ttcaccgtca tccccgaaac
     8821 gtgcgaggca gctgcggtaa agctcatcgg cgtggtcgtg aagcgattca caaatatcgg
     8881 cctgttcatc tgcgtccagt tcgttgagct tctccagcag cgttaatgtc tggcttctga
     8941 taaagcgggc catgttaagg gcggtttttt cctgtttagt cactgatgcc tccgtgtaag
     9001 ggggatttct gttcatgggg taatgatacc gatgaaacgc gagaggatgc tcacaatacg
     9061 ggttaatgat gatgaacatg cccggttact tgaacgctgt gagggtaaac aactggcggt
     9121 atggatgcgg cgggtctgcc tgggggagcc ggttgcccgt tccggaaaac tgccgacact
     9181 ggcaccgccg ttactgcgtc agctggccgc catcggaaat aacctgaatc agacagcccg
     9241 taaggtgaac agcgggcagt ggtcttccgg tgaccgggtt caggtggtgg ccgcactgat
     9301 ggccatcggg gatgagctgc gccggctgcg tctggccgtc agggaacagg gggcgcggg
//
