LOCUS       NZ_CP122502            15625 bp    DNA     circular CON 21-JUN-2024
DEFINITION  Escherichia coli strain W409 plasmid unnamed3, complete sequence.
ACCESSION   NZ_CP122502
VERSION     NZ_CP122502.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN34133715
            Assembly: GCF_030168715.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15625)
  AUTHORS   Yang,J.
  TITLE     Genomic insights into global blaCTX-M-55-positive Escherichia coli
            epidemiology and transmission characteristics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15625)
  AUTHORS   Yang,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2023) Veterinary college, South China Agricultural
            University, Wushan, Guangzhou, Guangdong 510000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. Version 10.0.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10X
            Sequencing Technology             :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030168715.1-RS_2024_06_21
            Annotation Date                   :: 06/21/2024 04:15:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,988
            CDSs (total)                      :: 4,869
            Genes (coding)                    :: 4,602
            CDSs (with protein)               :: 4,602
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 267
            CDSs (without protein)            :: 267
            Pseudo Genes (ambiguous residues) :: 0 of 267
            Pseudo Genes (frameshifted)       :: 86 of 267
            Pseudo Genes (incomplete)         :: 190 of 267
            Pseudo Genes (internal stop)      :: 42 of 267
            Pseudo Genes (multiple problems)  :: 45 of 267
            Pseudo Genes (short protein)      :: 1 of 267
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP122502.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..15625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="W409"
                     /host="chicken"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="China:Guangdong"
                     /collection_date="2004"
     gene            join(15554..15625,1..417)
                     /locus_tag="QC809_RS24870"
     CDS             join(15554..15625,1..417)
                     /locus_tag="QC809_RS24870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_063084439.1"
                     /translation="MPCCFRPLQARTVSERQAKWLTDRVQTIRGLKSPNEPQRLLVILH
                     DKADKTAQEIKTLSLLIQAERAAEKAQEARTKVMNLIQAEKRAEAKAARKARDHALYQS
                     AGLLIMAGLVDSQTGKPVDDPAKLLGALVSLHELSRDNPKWEEWKLKGQVLLNQKKED"
                     /name="conjugal transfer protein TraD"
     gene            1016..1972
                     /locus_tag="QC809_RS24875"
     CDS             1016..1972
                     /locus_tag="QC809_RS24875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293214.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001293214.1"
                     /translation="MSELVVFKANELAVSRYDLTEHETKLILFCVAKLNPTIENPTRDE
                     LTVKFSCSEYARTMGLSYENAWGRLNSATSDLFKRSVELIYPTGAVSKRIFNWTEYAEF
                     NREEQTVTLVFSSYIQPLLFHLKKFIKYNLEHVKAFENKYSMRIYEWLLKELSQRKTHR
                     GNIEISIKEFKFMLMLEKNYPLYAELNRWILKPVTNDLNTYSNMKLTIEKRGRPADTLI
                     FQVELDKQIDLVTELAKDPASKKEDKTIRLTPENRLHEGLKTTLHDALTAKIQLTSFEA
                     KFLSDMQSKYDLNGSFTWLTQKQRTTLEKILAKYGRI"
                     /name="replication initiation protein"
     gene            1980..2069
                     /locus_tag="QC809_RS25005"
     CDS             1980..2069
                     /locus_tag="QC809_RS25005"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_325914343.1"
                     /translation="MALISVTEAARLTGKNRATIHRYIKQGKL"
                     /name="helix-turn-helix domain-containing protein"
     gene            2138..>2737
                     /locus_tag="QC809_RS24880"
                     /pseudo=""
     CDS             2138..>2737
                     /locus_tag="QC809_RS24880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612097.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            complement(2935..4512)
                     /locus_tag="QC809_RS24885"
     CDS             complement(2935..4512)
                     /locus_tag="QC809_RS24885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="WP_001323889.1"
                     /translation="MRQNLPVTGRNLELPKDANILSTTSPQSHITYVNPDFIKISGFTE
                     EELLGQPHNIVRHPDMPPAAFEHMWSTLKSGRSWMGLVKNRCKNGDHYWVSAYVTPIAK
                     NGSIVEYQSVRTKPEPEQVLAAEKLYAQLRSGKAARPKLAASFSVKILLLIWGSIISSA
                     MAAGMLTDTSISSLLLATLMSGSLSSVSVLAILSPLGRLVERARNISNNPLSQSLYTGR
                     TDEFGQIEFALRMMQAETGAIVGRIGDASNRLSEHTRGLLKDIESSNVLTVEQQAETDQ
                     IATAVNQMVASIQEVASNAQHAADAAGRADTETASGQRLVAHTSQSITALEGEIRQATQ
                     VIHELEGQSNEISKVLDVIRGIAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRSLAA
                     RTQQSTTDIQSMISALQERAQSAVTVMEQSSRQAHTSVAHAEEAATALDGIGQRVNEIT
                     DMNAQIATAVEQQGAVSEDINRSIINIRDAADTNVQTGQNNLQSAKSVAQLTSALSELA
                     KQFWEKRG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     gene            4822..5382
                     /locus_tag="QC809_RS24890"
     CDS             4822..5382
                     /locus_tag="QC809_RS24890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220837.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001161490.1"
                     /translation="MQGHRIGYVRVSSFDQNPERQLEQTQVSKVFTDKASGKDTQRPQL
                     EALLSFVREGDTVVVHSMDRLARNLDDLRRLVQKLTQRGVRIEFLKEGLVFTGEDSPMA
                     NLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKALSDEQAATLRQRATAGEP
                     KAQLAREFNISRETLYQYLRTDD"
                     /name="recombinase family protein"
     gene            5386..8351
                     /locus_tag="QC809_RS24895"
                     /pseudo=""
     CDS             5386..8351
                     /locus_tag="QC809_RS24895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /name="Tn3-like element TnAs1 family transposase"
     gene            <8388..8953
                     /locus_tag="QC809_RS24900"
                     /pseudo=""
     CDS             <8388..8953
                     /locus_tag="QC809_RS24900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            complement(9215..10348)
                     /locus_tag="QC809_RS24905"
     CDS             complement(9215..10348)
                     /locus_tag="QC809_RS24905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="WP_063084436.1"
                     /translation="MKISIIGNTANAMILFRLDLIKTLTKKGISVYAFATDYNDSSKEI
                     IKKAGAIPVDYNLSRSGINLANDLWNTYLLSKKLKKIKPDAILSFFSKPSIFGSLAGIF
                     SGVKNNNAMLEGLGFLFTEQPHGTPLKTKLLKNIQVLLYKIIFPHINSLILLNKDDYHD
                     LIDKYKIKLKSCHILGGIGLDMNNYCKSTPPTNEISFIFIARLLAEKGVNEFVAAAKKI
                     KKTHPNVEFIILGAIDKENPGGLSESDVDTLIKSGVISYPGFVSNVADWIEKSSVFVLP
                     SYYREGVPRSTQEAMAMGRPILTTNLPGCKETIIDGVNGYVVKKWSHEDLAEKMLKLIN
                     NPEKIISMGEESHKLARERFDANVNNVKLLKILGIPD"
                     /name="glycosyltransferase family 4 protein"
     gene            complement(10348..11157)
                     /locus_tag="QC809_RS24910"
     CDS             complement(10348..11157)
                     /locus_tag="QC809_RS24910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788525.1"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="WP_000788525.1"
                     /translation="MKNHKVSIIIPCFNNEKTIERCVLSALKQTHRNIEIICINDGSSD
                     NSWNILKKLSSLYGNVFAFNNEDNSGPSFSRNKGVSLSTGHFLSFLDADDYWHPKKLEL
                     QLSFINDENLDFLGSTCSIGEKNNQEINQEIKKEHLKLKIISFNMMLFKNYFQTPAVIM
                     KRDIFIPFNENQRFSEDYMSWLVIVYNKKNKCGLIYGRDLVFLDKFNFGVSGLSGNLWL
                     MEKWELKNIFNFLLKGKIMAVPAILFSLIKYGRRCALTKKNKGKGNK"
                     /name="glycosyltransferase family 2 protein"
     gene            complement(11154..12053)
                     /locus_tag="QC809_RS24915"
     CDS             complement(11154..12053)
                     /locus_tag="QC809_RS24915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_224710277.1"
                     /translation="MWFIFFFKRKPIHLSYLIALGLLFVSSFLLIKLFQLGFGDWGRLT
                     VPVFYDNSWSYFPLGYESSSDPNVLAFMLCIGSIYMFFTLPNKYTINIIFILSMMCSFL
                     TLSRSSFLSFFISFLIVSVYSFFVLKKTQLRLLALIMISLAMSFVVMKNIPAVVTENNG
                     ASFVTEPNITERIVSKASNSDRIARLKNAIVEISNLKTMFIGNGIGYSTLTQDPHNFYL
                     STIIDSGILILAIVMMVLTKPLLMVSRTPAKSNVILGCMFLTIYYMFISLFYWQVRVLY
                     FVIILLFTFVNISKKSEQ"
                     /name="hypothetical protein"
     gene            12441..13667
                     /locus_tag="QC809_RS24920"
     CDS             12441..13667
                     /locus_tag="QC809_RS24920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080803.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="WP_063084438.1"
                     /translation="MNRLFSNAFWLMASQIGGLLIPLIELPLLARRLGADEYGKVVYVL
                     AISIMASVFIEYGFNFSAVKKIAGRKDDKYISQISTDVFLAKIILSIIPIILALTLIYT
                     TSSGISIPNNWFVWIILFILSFGFTPFWYYISIGNIIFPAILDLLLRVTGLILIVIFVN
                     SPSDAKTTIAIQSMVGALNTLISTAVVFYLSGVGAINIKRALNEIKDGFSVFLYKSSQG
                     IMITMSSSLLGFFGGAKSVGFFVPSEKVTKVITTLMITMLNVAYPHFVKNLSDSKNNIR
                     HIKKTILNISAILLFLSLIFSFFVFVFSDKIIFFVFGIEYERAIPLIKTIIWVIPLRSW
                     VLSLTILWFIPINKEKFITKITLVNIMLISFTACLFIYLFGTIGIVMSILLSEIITFVI
                     LTYFFFKEN"
                     /name="oligosaccharide flippase family protein"
     gene            complement(13869..14453)
                     /locus_tag="QC809_RS24925"
     CDS             complement(13869..14453)
                     /locus_tag="QC809_RS24925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039052609.1"
                     /translation="MSDEIKKIAALIGHQQAQEKRVAALIEEFQAESAKLSRQTAQLAQ
                     VIHELDSASGKMTDTIRRSVNAALTQVEKELKQTGLAQQKPAVNALNQVVTAANESVSA
                     MRREMSRYTWKSAIYIALTIFAVMGGCLWGMYYFIDSGYDRIAKMQRMEAVWQKKAPLA
                     DISTCDGKPCVKVDTRQSYGDKKDTYLIIKQ"
                     /name="hypothetical protein"
     gene            complement(14466..15560)
                     /locus_tag="QC809_RS24930"
     CDS             complement(14466..15560)
                     /locus_tag="QC809_RS24930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_284654832.1"
                     /translation="MASYHLSVKTGGKGKAASHADYIAREGKYAREKDNDLEHQESGNM
                     PAWAAHKPSEFWKAADTFERANGCTYREIEIALPREFTPAQRLELVRDFVQQEIGDRHA
                     YQFAIHNPRAAIEGGEQPHAHIMFSERLNDGIERDPQPYFKRANTKNPERGGAKKVRFG
                     ETPTERKAYLIAQRERWADLQNKHLERYQHIDRVDARSLKAQGIEREPERHFGAGQVRQ
                     FEPAQLHALMERREAERQAQHCRDECNSVIDVTTTLREALSERDNLTLKHEQMSEPQLK
                     ATSDKAFDFEKESDKLNALVSDAMKDIQEEIDLQTLVNDAMAEFQGIHQDMVKAEQQRQ
                     QEKERQRLAEQKRQKSDKGWSFSR"
                     /name="MobA/MobL family protein"
     Typing          1017..1972
                     /name="rep_cluster_1155"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.582"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(14470..15560)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="85.714"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtgcaaacta tccgtggttt aaaatcaccg aatgagccac aacgcttatt ggtgattttg
       61 cacgataaag cggataaaac cgcccaagaa ataaaaacgc tctcattgtt aattcaggcg
      121 gagcgggcag cagaaaaagc ccaggaagcc cgcacaaagg taatgaatct tattcaagca
      181 gagaagcgtg cagaagccaa agctgcccgt aaagcacgtg accatgccct ttatcaatca
      241 gcggggttac tcatcatggc ggggttagtg gacagtcaaa cgggtaaacc ggtagatgac
      301 cccgcaaaac tactgggtgc attagtcagc ttgcatgaat tatcccgtga taatcccaaa
      361 tgggaagaat ggaaattgaa ggggcaggta ttactcaatc agaaaaagga agattagagc
      421 acagtgttaa taccctctaa aacgccacag aaagtccgtg gcgaggtttt gttggtttga
      481 taatggaatt tatcacctaa tactgaaaac atggcacaga agcgcaaaca accaccttcc
      541 tgattgagcc gtgagcgaag cgaacccaat gacacgaaaa aaacagcggc gctgtggctg
      601 gatggtcgga gacaaaagaa aggggcagcg cgttgcccga gcaaagcgag ggagctactt
      661 aggtgtttta ggtttgtctt gaaaaggagc aaacgttgtt tgcgacatcc ttttaactac
      721 ggcaaatcga cggcaatttt ctgatttatc cacagggctg ggatctattc tttttttatc
      781 ttttatttat tctttcttta ttctaataat tttaacttat tgttttttaa taaaaaaaaa
      841 agcattaaat gtaggaaaaa ttaggctaga tgtaggaaaa attaggctag cagtgggaaa
      901 gatgtaggaa aaattaggtt attcacaatc aaacccgaaa aaatgtagtt gatttaaatc
      961 gctacatcat tatgattcat gtagcgattt aaattactac ataatggtga ttagcatgtc
     1021 tgaattagtt gtgtttaaag caaatgaatt agcagtaagc cgttatgatc taactgaaca
     1081 tgaaaccaag ctaattctgt tttgcgttgc aaagttgaac cctacaattg aaaacccaac
     1141 aagggatgaa ttaacagtta aattctcatg ttctgaatat gcacgcacta tgggcttaag
     1201 ttatgaaaat gcttggggaa gattgaacag tgcaactagt gatttattta aacgctctgt
     1261 tgaattaatt tatcctacag gagcagtttc taaacgaatt ttcaattgga cggaatacgc
     1321 tgagttcaat agagaagaac aaactgtaac gttagtattt agctcatata ttcaaccact
     1381 tttatttcat ttaaaaaaat tcattaaata caaccttgag catgttaaag cctttgaaaa
     1441 taaatactca atgcgaatat atgaatggtt actaaaggag ctttcacaaa gaaaaacgca
     1501 taggggaaac attgagataa gtatcaagga atttaagttt atgctcatgc ttgaaaaaaa
     1561 ctacccttta tatgcagaat tgaatcgctg gatcctaaaa ccagttacaa atgacttaaa
     1621 cacttacagc aatatgaagt tgactattga aaaacgcggt cgtcctgctg acacactaat
     1681 ctttcaagtt gaactggata aacaaattga ccttgtgact gaactagcaa aagatcccgc
     1741 atcaaaaaaa gaagataaga caatccgttt aacgcctgaa aatcgtcttc atgaggggct
     1801 aaaaacaaca ttgcatgatg ctttaactgc aaaaattcaa ctgactagtt ttgaagcaaa
     1861 attcctgagc gatatgcaaa gcaagtacga tcttaacggc tcatttacat ggctaactca
     1921 aaaacaacga actacgctag agaaaattct ggctaaatac gggcggatat gaggtatcta
     1981 tggcactgat atcagttaca gaggcagcca gactaactgg aaaaaacaga gcaacaatac
     2041 atcgctatat taagcaagga aaactgtagt ggcatactga atttggccac ctgaatagag
     2101 gtgatatcat cacttcgcag taaaaacagg tgacattatg accggacgta acagacgcaa
     2161 ttttagcccc gggtttcgcc tcgaagctgc ccagctttta ctcgatcagc attacaccgt
     2221 tgccgccgct gctacggcaa tgaacgtcgg caaatccaca atggacaaat gggttcgaca
     2281 actgaaagga gagcgagcgg gaaaatcccc cactacttca cccatcacct gagcagattg
     2341 aaatacgcga gctaaagaaa agacttcaac gtgttgaaat ggaaagggat atattaaaaa
     2401 atgctaccga gaccttgatg tcagactccc taaacaattc tcattagttg agaaactcag
     2461 ggcgcagttt cctgttgcca ttgtgtgcaa cgtgtttggg gttcatcgca gcagttataa
     2521 atactggcgg cagcacggga agcctgacgc cacgagagtg gcattactga gccttgtgcg
     2581 tgaagtttat cgcgaaagta acggctcagc aggagcgcga agcattaccg cgatggtcac
     2641 caccaaaggt ataaaactga gccgctgacg ggcaacaaag ctaatgaaag cgctcaatat
     2701 tatcagctgt cagcaacctg tccatcgtta taagaaggcg tctaaggggg aaccgcagaa
     2761 ttcggaaaaa atcgtacgct aagctaacgg tgttctcgtg acagctcttt gactaggctt
     2821 tctaaggcca ttctgatagc cctgacttcc tgaaaagcca tggctaaaat ttgtgcggct
     2881 aaaagggata accgatggta aagtaagtta tccctgtcga gatactgaaa agcgttatcc
     2941 tcgtttttcc caaaactgtt ttgccagttc gctcagagcg ctagttaact gagcgacaga
     3001 tttcgcactt tgcaaattat tctgcccggt ctgtacattg gtatcagcag catcgcgtat
     3061 attgataata ctgcggttta tgtcttcact tactgctccc tgctgctcga ccgcagtcgc
     3121 tatttgcgcg ttcatgtcgg taatttcgtt aacgcgttgg ccaattccat caagagctgt
     3181 agctgcttcc tctgcgtgag ctacactcgt gtgcgcttgc cgactacttt gctccatgac
     3241 tgtaacagcg gattgcgctc gctcttgtag agcgctgatc atgctttgaa tatccgttgt
     3301 cgattgctgt gtgcgagcag caagactgcg aacctcatcg gcgacaacag caaaaccacg
     3361 cccctgctca ccagcacgcg cggcctcaat tgctgcgttg agtgccaaca aattcgtttg
     3421 ctcggcgatc cctcgtataa cgtcaagaac ttttgatatc tcgttacttt gaccttcaag
     3481 ctcatgaata acctgagtgg cttgcctaat ttcaccttca agggcagtga ttgactggct
     3541 tgtgtgggct accagacgct ggccagatgc cgtctcagtg tctgctcttc cggccgcatc
     3601 tgcagcatgc tgtgcattgc tcgcaacctc ttgaatgctt gccaccattt ggtttactgc
     3661 cgttgctatt tgatctgtct ctgcctgctg ctcaactgta agtacattgc ttgactcaat
     3721 atcctttagt aggcctcggg tgtgttcgct aagccgattt gatgcatcac ctatgcgacc
     3781 tactatggcg cctgtttcag cttgcatcat tcgtaaagca aactctattt ggccaaactc
     3841 atcggtgcgc ccagtgtaga gggattgact taatgggtta ttggaaatat tcctggctct
     3901 ttcaaccagt cttccaagag gagagagaat agccaaaaca ctaacagagc ttaagcttcc
     3961 tgacattaaa gtggctaaca ataagctgct tattgatgta tcagtaagca tgccggcagc
     4021 cattgcgctt gatataatac taccccatat gagcaagagt attttcacgg aaaagctagc
     4081 agccaatttc ggcctcgcgg ccttcccgct tctcaattga gcatataatt tttccgcagc
     4141 caaaacctgc tcaggttcag gcttggtcct tacagactgg tattcaacaa tcgaaccatt
     4201 cttagctatt ggcgttacat aagcacttac ccaatagtgg tcgccatttt tacagcgatt
     4261 ttttactagc cccatccatg agcggccaga ttttaatgta ctccacatat gctcaaatgc
     4321 agcaggcggc atatctgggt gtcttacgat gttgtgaggc tggcctaata gttcttcctc
     4381 agtgaaacca ctgattttaa tgaagtcagg attaacgtac gtgatatggc tttgagggga
     4441 ggtagtcgaa agaatattgg catcttttgg gagttctaag tttcgacccg tcactggtaa
     4501 attctggcgc ataagaacct caagggttgg ctgttttatt ttattgtttt cggcattaag
     4561 cccaatttct tgagcgttac gataaagcta gcatggaaac gataggtgca agcaagttaa
     4621 gggttgcatc gcgcatgtca atctaggcta taccctaact tgatgtcagg cagggccgcg
     4681 ccgcttcgtc agaatagagt ctgctttccc attttttgac acatgcccgc gaaggttata
     4741 gatttcagcc tgacagaaat gggctttgag gcacaacgga acagaaagtg cacttaagcc
     4801 gccttcaacc aaggagacat cgtgcagggg caccgcatcg gctacgtccg ggtcagcagc
     4861 tttgaccaga acccggaacg ccagctggaa caaacccagg tgagcaaggt gttcaccgac
     4921 aaggcatcgg gcaaggacac ccagcgcccc cagctcgaag cgctgctgag cttcgtccgc
     4981 gaaggcgata cagtggtggt gcacagcatg gatcggctgg cccgcaacct cgatgacctg
     5041 cgtcgcttgg tacagaagct gactcagcgc ggcgtgcgca tcgagttcct gaaggagggc
     5101 ctggtgttca ctggcgagga ctcgccgatg gccaacctga tgctgtcggt gatgggggcc
     5161 ttcgctgagt tcgagcgcgc cctgatccgc gagcggcagc gtgagggcat cgccttggcc
     5221 aagcagcgtg gcgcgtaccg gggccgcaag aaagccctgt ccgatgagca ggctgctacc
     5281 ctgcggcagc gagcgacggc cggcgagccc aaggcgcagc ttgcccgcga gttcaacatc
     5341 agccgggaaa ccctctacca gtacctccgc acggacgact gacacatgcc gcgtcgcttg
     5401 atcctctcgg ccacggagcg ggacaccctg cttgcgctgc cggaaagcca ggatgacctg
     5461 atccgctact acaccttcaa cgactccgac ctgtcgctga tccgccagcg acgcggcgac
     5521 gccaaccgcc tcggcttcgc cgtgcagctc tgcctgctgc gctaccccgg ttacgcgctg
     5581 ggaaccgaca gcgagctgcc cgagccggtc atcctgtggg tggcgaagca agtccaggcc
     5641 gagccggcga gctgggcaaa gtacggcgag cgcgacgtga cccgtcgcga gcatgccccg
     5701 gaactgcgca cctacctgca actggccccg ttcggcctgt ccgacttccg cgccctggtg
     5761 cgcgagctaa ccgagctggc ccagcagacc gacaaaggct tgctgctggc cggtcaggcc
     5821 ctggagagcc tacggcagaa acgacgcatc ctgccggcgc tgagcgtgat tgaccgggcc
     5881 tgctcggaag ccattgcgcg agccaatcgg cgggtctacc gcgccctggt cgaaccactc
     5941 acggactcgc atcgggccaa gctggacgag ctgttgaagc tcaaggccgg cagcagcatc
     6001 acctggttga cctggctgcg ccaggcaccg ctgaaaccca actctcggca catgctggaa
     6061 cacatcgagc ggctgaagac atttcagttg gtggacttgc ccgaaggcct gggccggcac
     6121 atccaccaga accgcctgct caagctggcc cgcgagggtg ggcagatgac gcccaaagac
     6181 ctcggtaagt tcgagccgca gcgccgctac gcgaccctgg ccgccgtggt gctggagagc
     6241 accgcgaccg tgatcgatga gctggtcgat ctgcatgacc gcatcctggt caagctgttc
     6301 agcggcgcga agcacaagca tcagcagcag ttccagaagc agggcaaggc gatcaacgac
     6361 aaggtgcgcc tgtactccag gatcggccag gcgctgctgg aagcgaagga aagcggcagc
     6421 gacccctatg ccgccatcga ggcggtgatt ccctgggacg agttcaccga gagcgtcagc
     6481 gaggccgagc tgctggcccg gccggaaggc ttcgaccacc tgcacctggt cggcgagaac
     6541 ttcgccaccc tgcgccgtta cacgccggcc ttgctggagg tgctggaact gcgcgccgcg
     6601 ccggccgcgc aaggcgtgct ggcagccgtg cagaccctgc gtgagatgaa cgccgacaac
     6661 ctgcgcaagg tgccggccga tgcacccacg gccttcatca agccgcgctg gaagccgctg
     6721 gtgatcaccc cggaaggcct cgaccggaaa ttctacgaaa tctgcgccct gtccgagctg
     6781 aagaacgccc tgcgctccgg cgacatctgg gtcaagggct cgcggcagtt ccgcgacttc
     6841 gacgactacc tgctgccggc cgagaagttc gccgcactca agcgcgagca ggccctgccc
     6901 ctggcgatca acccgaacag cgaccagtac ctggaagagc gtttgcagct gctggacgag
     6961 cagttggcca ccgtcacccg cctggccaag gacaacgagc tgcccgatgc catcctcacc
     7021 gagtcagggc tgaaaatcac cccgctggat gcggcggtgc cggatcgggc gcaggcgctg
     7081 atcgaccaaa ccagccagtt actgccgcgc atcaagatca ccgaactgct gatggacgtg
     7141 gacgactgga cgggcttcag ccgccacttc acccacttga aggacggggc cgaggccaaa
     7201 gacaggacgt tgctgctgtc cgcaatcctc ggtgatgcga tcaacctcgg gctgaccaag
     7261 atggccgagt cgagccccgg cctgacctac gccaagctgt cctggctgca agcctggcac
     7321 atccgcgacg aaacctattc ggcggccttg gccgagctgg tcaaccacca gtatcgccac
     7381 gcctttgccg cccactgggg cgacggcacg acctcatcct ccgatggcca gcgcttccgc
     7441 gcgggtggcc ggggcgagag caccgggcac gtcaacccga agtacggtag cgagccggga
     7501 cggctgttct atacccatat ctccgaccag tacgcgccgt tcagcacccg cgtggtgaat
     7561 gtcggcgtcc gcgattccac ctatgtgctc gacggcctgc tgtaccacga gtccgacctg
     7621 cggatcgagg agcactacac cgacacggcc ggcttcaccg atcacgtctt tgccctgatg
     7681 cacctgctag gcttccgctt cgcgccgcgc atccgcgacc tcggcgaaac caagctgtac
     7741 gtgccgcagg gcgtgcaagc ctacccgacg ttgcgcccgc tgatcggcgg caccctgaac
     7801 atcaagcacg tgcgtgccca ctgggacgac atcctgcgcc tggccagctc gatcaagcag
     7861 ggcaccgtca ccgcctcgct gatgctgcgc aagctcggca gctacccgcg ccagaacgga
     7921 ctggccgtgg ccctgcgcga gctgggccgg atcgagcgca cgctgttcat cctggactgg
     7981 ctgcaaagtg ttgaactgcg ccgccgcgtg catgccggcc tgaacaaagg tgaggcgcgc
     8041 aactcgctgg ccagggcggt gttcttcaac cgccttgggg aaatcaggat cggagcttcg
     8101 agcagcagcg ctaccgggcc agcggcctca acctggtgac ggcggctatc gtgctgtgga
     8161 acacggtgta cctggaacgc gccacccagg ggttggtcga ggccggcaag ccggtggacg
     8221 gcgagctgct gcaattcctg tcgccgctgg gctgggagca catcaaccta accggcgatt
     8281 acgtctggcg gcagagccgc agactggaag acgggaagtt tcggccctta cggatgcccg
     8341 gaaaacctta gcgtacgatt ttttccgaat tctgcgggct cccctctaag gaacacattg
     8401 agatccctaa ttatctggat cgccagtttg ctgttaccga gcctaatcac gcctggtgcg
     8461 gtgatgtgac ttatatctgg acgggaaaac gccgggctta tctggtgtag tactcgattt
     8521 gttttcccgt aaaccagctg gctgggcgat gtcatttttc cctgattcag cactgacagc
     8581 taaagccctg tcgatggcct gggaagcacg gggaaaaccg gataatttac tgtatcactc
     8641 ggatcaaggc agccactata ccagcaggaa tttcagtcag ttactgtgga gatatcagat
     8701 aaagcaaagt ctgagtcgcc ggggaaattg ctgggataac agcccaatgg aacggttctt
     8761 cagaagcctg aaaacagagt gggtaccgga taatggctac gctaatttta gccaagccag
     8821 catggcaata acgaattaca tcacaggata ttacagccag ctcagacctc atcaatataa
     8881 tggtggtttg acgccgaatg aatcagaacg attgttcttg aaaaactcta aagccgtggc
     8941 cagtttttgt tgaccactac acaatgttgt attttgcata cagcataatc gactggaata
     9001 aaattgtatt ggaataggct ctaacactga tataacggca gtttagaacc tatctcaata
     9061 gggattaatt accgcataaa aatatattat cactgtaacc aaaacctcat acagcctgac
     9121 ttttaaacat cgatatggct gggtaaggtc atgaatttag ctcctattga gataggttct
     9181 tagttccgaa tgaatcagag ccgcttttcg tttattaatc aggaattcct agtattttta
     9241 ataactttac attatttaca tttgcatcga atctttctct tgctaactta tgactttctt
     9301 ctcccatact gattattttt tcaggattat taattaactt cagcattttt tctgcaagat
     9361 cttcatgtga ccattttttt acaacatatc cattcacacc atcaataatt gtttctttgc
     9421 agcctggtaa attagtagtt aaaatcggtc tccccatagc catcgcttct tgtgtactac
     9481 gaggaactcc ctctcgataa taggaaggaa gaacaaatac gcttgatttt tcaatccaat
     9541 cagccacatt agaaacaaat ccgggataag aaataactcc tgatttaatt aaagtatcta
     9601 cgtcagattc agataaccct ccggggtttt ccttatctat agcgccaagt ataataaatt
     9661 caacattggg atgtgttttt tttatttttt ttgcggcagc aacaaactca ttgactcctt
     9721 tttctgctag caaacgagct ataaaaatga atgatatttc atttgttggt ggcgtgcttt
     9781 tacagtaatt attcatatct aaaccaatgc caccaagaat atggcaagat tttaatttta
     9841 ttttgtattt atctatcaag tcatgataat catccttgtt aagaagtatt aatgagttga
     9901 tatgtggaaa tattatttta tacaggagaa cctggatatt tttaagtaac tttgttttta
     9961 acggagttcc atgtggctgc tctgtaaata aaaaacctaa cccctcaagc atagcgttat
    10021 tatttttaac gcctgaaaaa ataccagcca acgatccaaa gatagagggc tttgaaaaaa
    10081 aagataaaat agcatctggt tttatcttct ttagtttttt acttaataag taagtattcc
    10141 ataaatcatt agcaaggtta ataccactgc gacttaaatt ataatcaaca ggaatggcgc
    10201 ctgctttttt tattatttcc ttggatgaat cattatagtc agtagcaaaa gcatagactg
    10261 aaatcccttt cttggttagt gtttttatta aatccaatct aaacaaaatc atagcatttg
    10321 ctgtgttccc tattattgag atcttcatta tttattaccc ttacctttat ttttctttgt
    10381 taaagcgcat cttcttccat attttatcaa agaaaacaag atcgcaggca ctgccattat
    10441 tttacctttc aacaagaagt taaatatatt ttttaactcc cacttctcca tcaaccataa
    10501 attaccactc aaccctgaca ctccaaagtt aaatttatcg agaaaaacca aatcccttcc
    10561 atatattaat ccacatttgt tttttttatt ataaacgata acaagccatg acatgtagtc
    10621 ctctgaaaaa cgctgattct cattaaatgg aataaaaata tctcttttca taatgacggc
    10681 tggagtctgg aaataattct taaacaacat catgttaaat gaaattattt ttaattttaa
    10741 atgttctttt ttaatttctt ggttaatttc ttggttattt ttctcaccaa tggaacacgt
    10801 tgaacctaaa aaatccaagt tttcatcatt aataaatgat agttgtaatt ctagtttttt
    10861 tggatgccag taatcatctg catccaaaaa tgacaaaaaa tgccccgtgg atagtgaaac
    10921 tcctttattc ctggaaaatg acggccctga attatcctca ttattaaaag caaaaacatt
    10981 tccatacaat gagcttaatt tctttaaaat attccagcta ttatctgacg atccatcatt
    11041 tatacaaata atctctatat ttctatgggt ttgttttaat gcggaaagaa cgcatctttc
    11101 tatagttttt tcgttattaa aacaggggat gataatacta accttatggt ttttcattgc
    11161 tcgctcttct tacttatatt tacaaaggta aataataata taataacaaa gtataggact
    11221 ctcacttgcc agtagaaaag agaaatgaac atatagtata tagtcaagaa catacacccc
    11281 aaaataacat ttgattttgc gggggttctt gaaaccatta ataatggttt tgttagcacc
    11341 atcattacaa ttgctaatat caaaataccg gaatcaataa tagttgataa gtaaaaatta
    11401 tgtggatcct gtgttaatgt actatagcca ataccattac caataaacat tgtctttaga
    11461 ttacttattt caacaatagc attttttaat ctggctattc tatcactatt acttgcttta
    11521 ctaactattc gttctgttat atttggttct gtgacaaatg aagctccatt attctcagtc
    11581 actacagcgg gaatgttttt catcactaca aaagacattg caagtgaaat cataattagt
    11641 gccaacaacc ttagctgtgt tttctttagc acaaagaatg aataaacact tacaataaga
    11701 aaggaaataa aaaaagataa aaaagaactc cgtgataagg tcaaaaatga acacatcatt
    11761 gacaatatga atataatatt aattgtgtat ttatttggca atgtgaaaaa catatagatt
    11821 gagccaatgc ataacataaa agctaacaca tttgggtcac ttgaactttc ataacccaaa
    11881 ggaaaataag accatgagtt atcataaaaa acaggaaccg ttaaacgtcc ccagtcacca
    11941 aagccaagtt gaaataattt aatcaacaaa aaagagctaa caaaaagcag gcctaaagca
    12001 attaaatatg ataaatgtat tggttttctt ttaaagaaga aaataaacca catagatatt
    12061 actatatata cagcatgact agtaattaga gtgatagatt tatgattata tacaaaaatg
    12121 ttaatgacaa ggtaagcaac tactaagcat aggagaaaaa tatgtgatct ttttataaaa
    12181 gactttcttt ttataagcca atagcaacct aatagtgtac aggaaaagag agtgtatact
    12241 gtaaccacat gatcactatt gactcccatg accacctgtt tattccctcc cataactgac
    12301 cacaatataa aaggaaaagt gcaaatgtat agataaaaac acatagtgat tcaactggtt
    12361 atttttattg taaataagat tcttctataa tatcataaac atgatgcctc actcaacatt
    12421 tttaacagat gatattgttt atgaacagac ttttttctaa tgctttctgg ttaatggcat
    12481 ctcagattgg cggccttcta atccccttga tcgaactgcc attgcttgcc agaagactgg
    12541 gggctgatga atatggaaaa gttgtatatg ttttagctat ttccataatg gcttctgttt
    12601 tcattgagta tggattcaat ttcagtgctg tgaaaaaaat cgcaggaagg aaagatgata
    12661 aatatatttc acaaatttca acagatgttt ttcttgctaa gataattctt agtattatac
    12721 ctattatttt agctctcacc ctaatataca caacatctag cggaatatct atacctaata
    12781 attggtttgt atggattatt ttatttatct tatcttttgg attcactccg ttctggtatt
    12841 acatatcaat aggaaatata attttccccg caatactcga tcttttacta agagtgacag
    12901 gtttaattct cattgttatt tttgttaact ccccgagtga cgcaaaaacc acaatagcca
    12961 ttcaatcaat ggtaggagcg ctaaatacat taatatcaac tgctgttgtt ttttatttgt
    13021 cgggagtagg agcaattaat attaaaagag cgcttaatga aattaaagat gggtttagtg
    13081 tatttttgta taaaagttca caaggaataa tgataaccat gtcatcatca ttattaggtt
    13141 tttttggggg ggctaaatct gttggttttt ttgttccatc tgaaaaagta actaaggtta
    13201 taacaacatt aatgataaca atgctaaacg tggcatatcc gcatttcgtt aaaaatctta
    13261 gtgatagcaa aaacaatata cgccacataa aaaaaacaat ccttaacata tcagcaatat
    13321 tattattttt atcattaatt ttttcttttt ttgtttttgt tttctccgat aaaataattt
    13381 tttttgtttt tggtattgaa tacgaaagag cgataccatt aattaagact attatttggg
    13441 ttataccgtt aaggtcatgg gtgttgtcac ttacaattct atggttcatt ccaataaata
    13501 aagagaaatt tatcacaaaa ataacactcg ttaatataat gctaatttcg ttcactgcgt
    13561 gtttatttat ttatctgttt ggtactatag gaatagtgat gtcaatttta ctatcagaaa
    13621 taataacttt tgtgatattg acctatttct ttttcaaaga aaactaagag cctatcccaa
    13681 aaggaattat cgtataatat aatgaattac gataatttct gagggcaacg tatggcaggc
    13741 aacaagtggc aaatcagtga tagactctgg gagaaaatgg ctcctctaat cctaaccatg
    13801 aaccgctcaa aaccacctcg ctactttgac gacttgcacg ggaaaacggc gtattcagcg
    13861 ccgttttttt attgtttgat aatcagataa gtgtcttttt tatcaccata tgactgcctt
    13921 gtatccactt tcacacaagg ttttccgtca cacgtggaga tatcagccag cggggctttt
    13981 ttttgccaga ccgcttccat gcgctgcatt tttgcaattc ggtcataacc gctgtcgata
    14041 aaataataca ttccccaaag acacccgccc atcaccgcaa atatcgtcaa tgcgatataa
    14101 atggcagact tccacgtata acgggacatt tcacgtcgca ttgcactcac ggattcatta
    14161 gctgctgtaa ccacctgatt cagcgcattg acggcgggtt tctgctgcgc caatcccgtt
    14221 tgtttgagtt ctttttcaac ctgagttaag gctgcattca ccgatcgcct gatcgtgtcg
    14281 gtcatcttac cggaggcact gtccaattca tggatcacct gagccaattg tgctgtttgg
    14341 cggcttaatt tggcggattc cgcctgaaat tcttcaatca acgccgccac acgcttttcc
    14401 tgcgcttgct gatgcccaat cagcgccgca atttttttaa tctcatcact catacaaaat
    14461 cctgttcatc gggaaaatga ccagccttta tcggactttt gtcgtttctg ctcggcgagt
    14521 cgctgccgtt ctttttcctg ctgacgctgt tgctcagcct taaccatgtc ctgatgaatg
    14581 ccctgaaact ccgccatcgc atcgttaaca agggtctgaa gatcgatttc ttcctgtatg
    14641 tccttcatgg catcactgac cagtgcgttc aacttgtcag actctttttc aaaatcaaac
    14701 gctttgtctg aagtcgcttt caactgaggc tctgacatct gctcgtgttt cagtgtcaga
    14761 ttatcacgct cgctgagggc ttcccgtagc gtggtggtaa catcaatcac gctgttacat
    14821 tcatcacgac aatgctgagc ctgtcgctcc gcttctctgc gctccatcag ggcgtgtaac
    14881 tgcgcgggtt caaactgcct gacctgtcct gcgccaaaat gtcgctccgg ctcacgctca
    14941 atcccctgtg ctttcaggga acgcgcatca acgcggtcga tatgctgata ccgttcaaga
    15001 tgcttattct gtaagtcagc ccagcgttcc cgctgggcga taaggtatgc cttgcgttcg
    15061 gtcggggttt cgccgaaccg cacctttttt gcgccgcccc gttcagggtt tttcgtgtta
    15121 gcccgtttaa aatagggctg cgggtcacgc tcgataccgt cattaagccg ttcagaaaac
    15181 ataatatgcg cgtgcggttg ttcaccgcct tcaatcgccg ctctggggtt atgaatggca
    15241 aattgataag cgtgccggtc gccaatttcc tgttgaacaa aatcgcgaac cagttcaagg
    15301 cgttgtgcgg gtgtaaattc acgcggcagg gcaatttcaa tttcgcggta ggtacagccg
    15361 ttggcacgtt caaacgtgtc agcggctttc caaaactcgg acggtttatg cgctgcccat
    15421 gcgggcatat taccggactc ttgatgctca aggtcattat ctttttcacg ggcatatttt
    15481 ccctcacgcg caatataatc ggcatgggaa gcggctttgc ctttcccgcc ggttttaacg
    15541 ctgagatgat aagatgccat gctgttttcg cccgctacaa gcgcgcaccg tttctgaacg
    15601 gcaagcaaaa tggctcacag acaga
//
