LOCUS       NZ_CP122447             3771 bp    DNA     circular CON 20-APR-2024
DEFINITION  Enterobacter ludwigii strain I9455333cz plasmid pI9455333_Col4401I,
            complete sequence.
ACCESSION   NZ_CP122447
VERSION     NZ_CP122447.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN34152342
            Assembly: GCF_029834565.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter ludwigii
  ORGANISM  Enterobacter ludwigii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Bitar,I.
  TITLE     mcr-10 producing Enterobacter ludwigii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Bitar,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2023) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Celera Assembler v. 8.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 35.0x
            Sequencing Technology :: hybrid, Illumina and Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029834565.1-RS_2024_04_19
            Annotation Date                   :: 04/19/2024 11:06:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,305
            CDSs (total)                      :: 5,189
            Genes (coding)                    :: 5,060
            CDSs (with protein)               :: 5,060
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 27 of 129
            Pseudo Genes (incomplete)         :: 100 of 129
            Pseudo Genes (internal stop)      :: 17 of 129
            Pseudo Genes (multiple problems)  :: 14 of 129
            Pseudo Genes (short protein)      :: 1 of 129
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP122447.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Enterobacter ludwigii"
                     /mol_type="genomic DNA"
                     /strain="I9455333cz"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:299767"
                     /plasmid="pI9455333_Col4401I"
                     /geo_loc_name="Czech Republic: Plzen"
                     /lat_lon="49.73 N 13.37 E"
                     /collection_date="2021"
     gene            complement(join(3351..3771,1..65))
                     /locus_tag="QCL67_RS26455"
                     /old_locus_tag="QCL67_26455"
     CDS             complement(join(3351..3771,1..65))
                     /locus_tag="QCL67_RS26455"
                     /old_locus_tag="QCL67_26455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000096578.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEKSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            complement(743..1063)
                     /locus_tag="QCL67_RS26460"
                     /old_locus_tag="QCL67_26460"
     CDS             complement(743..1063)
                     /locus_tag="QCL67_RS26460"
                     /old_locus_tag="QCL67_26460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_028120711.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRKTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAVLEKGGDD
                     DR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1704..1834
                     /locus_tag="QCL67_RS26465"
                     /old_locus_tag="QCL67_26465"
                     /pseudo=""
     CDS             1704..1834
                     /locus_tag="QCL67_RS26465"
                     /old_locus_tag="QCL67_26465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            2398..2589
                     /locus_tag="QCL67_RS26470"
                     /old_locus_tag="QCL67_26470"
     CDS             2398..2589
                     /locus_tag="QCL67_RS26470"
                     /old_locus_tag="QCL67_26470"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_028120709.1"
                     /translation="MLKKNEIILIKSCGNSKTKIFLRGMDEGLMIDASFEDIKFAINNN
                     LHDLLGIPAETTKLKLAK"
                     /name="hypothetical protein"
     gene            complement(3135..3347)
                     /locus_tag="QCL67_RS26475"
                     /old_locus_tag="QCL67_26475"
     CDS             complement(3135..3347)
                     /locus_tag="QCL67_RS26475"
                     /old_locus_tag="QCL67_26475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(1172..1351)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="88.525"
                     /coverage="83.0"
                     /evalue="1.6099999999999998e-54"
     Typing          1298..1813
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.7"
                     /coverage="99.0"
                     /evalue="1.36e-123"
     Typing          complement(2..780)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.0"
                     /coverage="93.0"
                     /evalue="3.7e-152"
ORIGIN
        1 gtactctgct gctgcgcttt cgattgctgc tccaagtcct tcgccagcgc taaaagactg
       61 ctcataaatg gctcccttca gtcggatgtt tcgccccccg tccgggtcgg caatgctgat
      121 actgtttttt gtggtgcgga ccacctcaaa acctgccgca gtgagcgccc cagtgacgtc
      181 ctcgcgcgtt ttgagttccc cggatgaggc taaggccagt aaaccccgtg taatcgcctg
      241 agcggcttcc agcttcgttt ccggcagtgc ggacggcgtg accagtaacc gccggttctc
      301 cggggcgttt ggatcatgca accccagtct gccgttcacc acggtctgcc aggcatcgat
      361 gcgcggccgg tcggcgcgat cgtaatacgg ctgcaggcgt ttcccggtca gcagttcagt
      421 gttggggatc aggaaattca gctccagccg ctccttgtcg gtgtgctcca cccacagaat
      481 gctgtactgg tctttatcaa gcccgggcat cagaacccgc tcaaagctgg ccatgatctg
      541 ctcccgctgc ccgggcggca gttcagcctc cgcgaacgac agcacaccgg aggtgtactt
      601 tttcgcatac ggcgaggcat cgataagctc ccggacttcc tccggttttc cctgcagcac
      661 ggtcgccccc tcgcgctgcc ggtcttttcc cagcaggtaa tccaccggac cggcaccacc
      721 accgcgtccc cggggatgaa atttaacgat catcatcccc ccctttttcc agcaccgcat
      781 gccgcagccg ctccagtccc gcatcgattg ccatcagggc ggccacaatc tgcacccggt
      841 catgaccggc accacctgca ttcactttcc gggcgatctg gttcaggttg ttacccatac
      901 cggcaagctg gcgcagcagc gccggcgata tcgatggcag tctgccggca cgcgccggct
      961 tctcatcgag gcaggtctta cgcatccacg ccgccagctg cttgccgtcg caccgctcga
     1021 gcagccgccg gtgttcgtct tccgtcaccc acatcgtgag catcttgttg cgtttatctg
     1081 ccagagtagc ctcctgataa caccgggacg ggcgggaaat ttttcccggg cggcacggtg
     1141 ttggacaagc cgcaggcgcg tcagtcgctt ttagcgggtt tcggggcgca gccctgaacc
     1201 agtcacgtag cgctagcgga gtgtatactg gcttagtatg ttacgacggg gtgggggttc
     1261 aggaaagtgc ttcatgtggc aaaggaaaaa tgcagctgcg gggcgtaagt gcgaaatgca
     1321 gtaggcagtg acatgacgcc tcctcgctca ctcggtcgct acgctccggc cgtgagactg
     1381 cggcgggcgt taccggctca cagataacga gaaaaagaat ggcggcctat ccggaaaggc
     1441 caaagagaag gatgcagcgt tgccgttttt ccataggctc cgccccccct gacaagcatc
     1501 acgaaatctg acgctcaaat cagtggtggc gaaacccgac aggacttaaa gatccccacc
     1561 gtttccgatg ggtcgctccc tcttgcgctc tcctgttccc gccctgcagt ttaccggtgt
     1621 ctgtccgctg ttaaaggccg cgtattctca ttccacgcct gacactcagt tctgtttagg
     1681 cagttcgctc caagctgggc tgtgtgcaag acccccccat tcagcccgac tgctgcgccc
     1741 tttccggtaa ctaacccttg agtccaaccc ggatggacac gcaaaaacgc cactggcagc
     1801 agccattggt aacaggatta gcagagcgaa ttaatgtagg tgatgctaca gagttcttga
     1861 agtggtggcc taactacagc tacactggaa ggacattatt tggtatctgc gctctgctga
     1921 agccagttac caggttaagc agttccccaa ctgacttaac cttcgaaaaa ccacctcccc
     1981 aggtggtttt ttcgtttctt ggacaagaga ctacgacgac aaatgatgga tctcaagaag
     2041 atccttttaa cgtacaaaaa tttccgttct ccacactacc caccatgagc acttaccaat
     2101 cataaaaatc atgataggcc actactgaat cggcacgggt ttaacagaca cctcagagtc
     2161 atttaagatg gcttaacgcc ccaataggcg gtgtaagtta actgtcgctg ccgcgtttta
     2221 cggcagtggt tgcagacaag cagggggggg ggcataccca caactcatac gcgttttttt
     2281 ttgtttttcc tgtatgtaca aaaaacataa ctaggaataa tctcaacaac cttcatcaac
     2341 atacgcaaaa aaacaacgat aatgcttcat ctgacgacaa cacacaatga caatgtcatg
     2401 ttaaaaaaaa atgaaattat cttaataaaa tcatgtggta actcaaaaac aaaaattttt
     2461 ttacgaggta tggatgaggg actaatgatt gatgcaagtt ttgaagatat aaaatttgct
     2521 atcaacaata acctccacga tcttctcgga atcccagccg aaaccacaaa actaaaacta
     2581 gcaaagtgat acagcccctg aagcatgaag gttcgaaata aagcaacata ttaaacataa
     2641 catccgttgc ccgcaccgct gaaacggggt tggctgcgtt tccggtgcca gacgcgagaa
     2701 agtggcgaca gacataacga aaaaacaccg gaaacgcagc cgctgaatat tacactggtg
     2761 catagtcatg cagccagatg cattgatgtt aaagtcacac taaaatccgc gccaatgcct
     2821 taaaaaccgg caaatgcctc tccagccagt cccacagccc ggccgccaga tacagacagt
     2881 taagtacagc ttcaatgacg ttaagcagtt tcataattcc ccccaggtat aggccgtgct
     2941 ctttttgatc acgaacctat ttagcgggag aaataagagc ccttcttcgt gcggcctgtc
     3001 accggatgat actgttcagc ccagcgtcgg accgttatag ctccgctgac gggaacgggc
     3061 ttccgccagc gctttttcgt gctgtcgctg ctcgtatgcc cgttcggcgg ccaccgccat
     3121 ttcatgccgc tgcactactg gctcaaacgc tgtaactgcc tgctcaagtt cgccacctgc
     3181 tgactcaagc gcgtcacgcg ctcgttcagc gccgcgttct cccgttgcat aagaccagac
     3241 atcttctgcc attccgcgaa ggcgctctcc cactcgtcca gcctttgcga gtagtcctga
     3301 tgtagctgct ctaatgcggt cagcaactgt ttttccagtt ccgtcatggg ctatttcctc
     3361 tccagtacca tccagttccc ttcctccccg taagctcccg ctttcgggtt cactttgacg
     3421 caggacagtt tcttctcccc gcagagcgac agctgcacgc cgtggttctt ctccgacagc
     3481 atggcctgcg tccgctcctg cgcccggata gtctggtaat tcccggtgat ctgctgcccc
     3541 tgccaccaca gaatgctccc gctcgtggcg atcagcagta ccgagaccag ggtgatggtc
     3601 agccacgtcc ggccgaccat acgcagcacg ctcaggcggt tcgactgcat cgccgcggtc
     3661 ataccctgct cgtgggcgct gatggcgtcg ctgattctct tcgcgcttgc gctcagttcc
     3721 gcttttacag acttctcgtg ctcgctgaat gcggctttca gcatctcgcc g
//
