LOCUS       NZ_CP122446             2495 bp    DNA     circular CON 20-APR-2024
DEFINITION  Enterobacter ludwigii strain I9455333cz plasmid pI9455333_Col,
            complete sequence.
ACCESSION   NZ_CP122446
VERSION     NZ_CP122446.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN34152342
            Assembly: GCF_029834565.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter ludwigii
  ORGANISM  Enterobacter ludwigii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Bitar,I.
  TITLE     mcr-10 producing Enterobacter ludwigii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Bitar,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2023) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Celera Assembler v. 8.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 35.0x
            Sequencing Technology :: hybrid, Illumina and Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029834565.1-RS_2024_04_19
            Annotation Date                   :: 04/19/2024 11:06:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,305
            CDSs (total)                      :: 5,189
            Genes (coding)                    :: 5,060
            CDSs (with protein)               :: 5,060
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 27 of 129
            Pseudo Genes (incomplete)         :: 100 of 129
            Pseudo Genes (internal stop)      :: 17 of 129
            Pseudo Genes (multiple problems)  :: 14 of 129
            Pseudo Genes (short protein)      :: 1 of 129
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP122446.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter ludwigii"
                     /mol_type="genomic DNA"
                     /strain="I9455333cz"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:299767"
                     /plasmid="pI9455333_Col"
                     /geo_loc_name="Czech Republic: Plzen"
                     /lat_lon="49.73 N 13.37 E"
                     /collection_date="2021"
     gene            1085..1312
                     /locus_tag="QCL67_RS26440"
                     /old_locus_tag="QCL67_26440"
     CDS             1085..1312
                     /locus_tag="QCL67_RS26440"
                     /old_locus_tag="QCL67_26440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1678..1863
                     /locus_tag="QCL67_RS26445"
                     /old_locus_tag="QCL67_26445"
     CDS             1678..1863
                     /locus_tag="QCL67_RS26445"
                     /old_locus_tag="QCL67_26445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2154..2372)
                     /locus_tag="QCL67_RS26450"
                     /old_locus_tag="QCL67_26450"
     CDS             complement(2154..2372)
                     /locus_tag="QCL67_RS26450"
                     /old_locus_tag="QCL67_26450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     Typing          complement(1911..1998)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          142..670
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 cagcgccgga ccagtcacgg agcactagcg gagtgtatac tggcttaaga tgtagcatgg
       61 cagtaacaca tagcattaat gccacataag acaggcgcag aaagtgaaag aaaacgcgtc
      121 aggagaaaat gtgatacaga atatattccg cttcctcgct cactgactcg ctccgctcgg
      181 tcgttcggct gcggcgagcg ggacgggctt acggatggat cagaaatttc ctgggggatg
      241 ccaggaagat acttaacgaa gaaagcataa ggccgcgcca aagccgtttt tccataggct
      301 ccgcccccct gacaagcatc acagaatctg acgctcaaat cagtggtggc gaaacccgat
      361 gcggcttaaa gataccaggc gtttccccct ggtggctccc tcgtgctccc ccgcattcct
      421 gcctttcggt ttaccggtgt cactccgctg ttatggccgc ggtttctcat tccacgcctg
      481 acactcagtt ccgggaaggc agttcgctcc aggatggact gtatgcacga accccccgtt
      541 cagtccgacc gctgcgcctt atccggtaac tatcaacttg agtccaaccc ggaaagacac
      601 gactaatcgc cattggcaac ggccattggc aaacaggatg tgcgagagat gtctcaagag
      661 ttcttgaagt ggcggcctta gtgaggctac actgaaagga caaatttggt gacatgtctc
      721 gcacaagact gttaccacgg ttaggggatc tgccaggatt acctaacctt cgaaaaacca
      781 cctgccaggg tggttttttc gtttacaggg caagagatta cgcgcggaaa aaaaggatct
      841 caagaagatc ctttataaat tagacatcca acctcacaaa gaggaatact taaatcatct
      901 tggtatatgt atcggccgga ttacatctca tataagcctc ctccccgcct gacaccacac
      961 tttgaccata gcacctcgcg cctaactcga tatacagccc accatcaagc ctcttttcac
     1021 ataaaaaatg acctatagtt agatggttag tgaccaaaac tgatatggat aaaaggagct
     1081 ggctttgaaa aagatttttg tttcagtgtt cgcagcggca gttgcgcttt cagccctcac
     1141 cggctgcacg cgcacaagct atgccataca caccaatgac ggtcgcacta ttgtcagcga
     1201 cggcaaacct aaagaatcag actcaggcct gcttggctat acagacgcga atggtgtcaa
     1261 acagcagatc aataaaaccg acgttaaaga agtctccgaa attccgcact gagcatcttc
     1321 tccgaaaaag gccgctaatg tggccttttt ctttacctgt ggttctccgc caaaatccca
     1381 gcaaattgca tcaccaaagc taaaagcttt cagggttgtc taaaaagcac aagacataag
     1441 aggaagtgcg gtattttata atcaagcccc caagaattta ccataacatc cgttgcccgc
     1501 accgcctgag acgcgttcag cgcgttcctg acgaaaccat gacaaaaacc acaacaaacc
     1561 accccggaac ccgtcagaaa cgcgcctgtt aaattttaac ggcatgcatg actatgcacc
     1621 agaatgacgc catgctcttt tcacgcaaaa atcatcacca gacggggaaa atcaccagtg
     1681 accagacagg aatccgccgc cctcaatatg gccaaattta tccgcgcaca gacacttctc
     1741 ctccttgagc ggctcgagca gatggatctg gatgaggctg ccggctgctg tgagcacctg
     1801 cacgatcagg ccgaagcgct ttacgccatg ctgaacgcac agataggcga ggaaaatgcg
     1861 tgaaaatcgg tgaacgggtg cgcaattcag tgcgcggccg tgaggcgatg gcggggtgtc
     1921 ggggcgcagc cctgaccagg gtatttgtga tgccggcgcg tgcgcggtat tacaaatgca
     1981 catcctgtcc cggaacggac accgggaaac agcaaaaaaa accgggcggc acgcccggaa
     2041 ctcaatcaag ttagattaga ttactctcac tcgtccataa cagcatcatg gaacgacgac
     2101 caccgtccgt gacggccgcc tcgtttaagt atggacagaa atacagaaaa tgctcaggac
     2161 gaaatgtaat gaatgcgaac ggattcaaga aattcgagca tgacagtcct tacggccggt
     2221 tcggtttcag acaaaatctg ccggtatgca tccagcatca tggctccggc atcccctccg
     2281 gcacgccgta gccagaccga aacaacggac acaagcaggt gtcgctcatc atcactaaga
     2341 gtcatcaggg ctccggaaga aaaaccaaac atggcttacc tactgttaac ggcaacgcag
     2401 aagaaaggtt tacagggtag cgggcaggga tgaaaaaaaa aaaattgcag tcaggccgga
     2461 acggctgctg aaccggtaca ggcgggttcc gggcg
//
