LOCUS       NZ_CP122445             4307 bp    DNA     circular CON 20-APR-2024
DEFINITION  Enterobacter ludwigii strain I9455333cz plasmid pI9455333_2,
            complete sequence.
ACCESSION   NZ_CP122445
VERSION     NZ_CP122445.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN34152342
            Assembly: GCF_029834565.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter ludwigii
  ORGANISM  Enterobacter ludwigii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Bitar,I.
  TITLE     mcr-10 producing Enterobacter ludwigii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Bitar,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2023) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Celera Assembler v. 8.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 35.0x
            Sequencing Technology :: hybrid, Illumina and Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029834565.1-RS_2024_04_19
            Annotation Date                   :: 04/19/2024 11:06:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,305
            CDSs (total)                      :: 5,189
            Genes (coding)                    :: 5,060
            CDSs (with protein)               :: 5,060
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 27 of 129
            Pseudo Genes (incomplete)         :: 100 of 129
            Pseudo Genes (internal stop)      :: 17 of 129
            Pseudo Genes (multiple problems)  :: 14 of 129
            Pseudo Genes (short protein)      :: 1 of 129
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP122445.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Enterobacter ludwigii"
                     /mol_type="genomic DNA"
                     /strain="I9455333cz"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:299767"
                     /plasmid="pI9455333_2"
                     /geo_loc_name="Czech Republic: Plzen"
                     /lat_lon="49.73 N 13.37 E"
                     /collection_date="2021"
     gene            complement(join(4159..4307,1..175))
                     /locus_tag="QCL67_RS26405"
                     /old_locus_tag="QCL67_26405"
     CDS             complement(join(4159..4307,1..175))
                     /locus_tag="QCL67_RS26405"
                     /old_locus_tag="QCL67_26405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_021243036.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            817..930
                     /locus_tag="QCL67_RS26410"
                     /old_locus_tag="QCL67_26410"
                     /pseudo=""
     CDS             817..930
                     /locus_tag="QCL67_RS26410"
                     /old_locus_tag="QCL67_26410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            1159..1596
                     /locus_tag="QCL67_RS26415"
                     /old_locus_tag="QCL67_26415"
     CDS             1159..1596
                     /locus_tag="QCL67_RS26415"
                     /old_locus_tag="QCL67_26415"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064183952.1"
                     /translation="MQQAIRMRIEADKAALDAGFTTNNFVGELGEKLAHAVYGGRILPN
                     SAKSADIQTDDGTLLQVKTRKPNKGKQFGSIRSWDFDYLIAITLGDKYEVIRALKIPVS
                     TCKELADHRAHTNSFIIKPTRKLLSHADIEDVTDKFQSVVL"
                     /name="hypothetical protein"
     gene            1848..2291
                     /locus_tag="QCL67_RS26420"
                     /old_locus_tag="QCL67_26420"
     CDS             1848..2291
                     /locus_tag="QCL67_RS26420"
                     /old_locus_tag="QCL67_26420"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="WP_064183953.1"
                     /translation="MIAVSLPDELLKRLDDAVDKTGKKRSFLIRESISMYLNNIENIHE
                     KKEIILNTSKPFYEVLIEEFKNEKKLITEARKTEFTMFSDNGKLYVINSKGNTRRLEPM
                     YVNNFFEEYKKTGSMSTSSYQDITFNSSYLLAALQYLMEKELI"
                     /name="ribbon-helix-helix protein, CopG family"
     gene            complement(2768..2983)
                     /locus_tag="QCL67_RS26425"
                     /old_locus_tag="QCL67_26425"
     CDS             complement(2768..2983)
                     /locus_tag="QCL67_RS26425"
                     /old_locus_tag="QCL67_26425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_064183954.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2990..3481)
                     /locus_tag="QCL67_RS26430"
                     /old_locus_tag="QCL67_26430"
     CDS             complement(2990..3481)
                     /locus_tag="QCL67_RS26430"
                     /old_locus_tag="QCL67_26430"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_064183955.1"
                     /translation="MSSLLTLAKDLEQQSKAQKQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRLSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<3516..4220)
                     /locus_tag="QCL67_RS26595"
                     /pseudo=""
     CDS             complement(<3516..4220)
                     /locus_tag="QCL67_RS26595"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          complement(277..463)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.158"
                     /coverage="87.0"
                     /evalue="2.62e-72"
     Typing          443..930
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.602"
                     /coverage="93.0"
                     /evalue="1.6700000000000003e-157"
     Typing          complement(3..175)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.828"
                     /coverage="98.0"
                     /evalue="1.56e-30"
     Typing          complement(2677..4169)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.8"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4166..4305)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.489"
                     /coverage="98.0"
                     /evalue="1.57e-19"
ORIGIN
        1 tattgcccat gccggcaagc tggcgcagca gcgccggcga gattgagggg agctttccgg
       61 tgcgcgcagg cttttcgtcc aggcaggtct gccgcatcca cgccgccagc tgcctgccgt
      121 cacagcgctc aagcagccgc cggtgctcgt cctccgtcac ccacatggtg agcatcttgt
      181 tccgtttctc tgacaaaccg gcctcctgat aacgccggga cgggcgggaa agcctttccc
      241 ggtcggcgcg gtgttggaca agccgcaggc gcgtcagtgg ttttagcggg tttcggggcg
      301 cagccctgaa ccagtcacgt agcgctagcg gagtgtatac tggcttaata tgttacggcg
      361 gagtggtaat gtatggaagt gcttcatgtg gctggggaaa aatgaggcta ccgggcgtaa
      421 gtgcaacatg cagataagga tgaaattacg cctcctcgct cactcggtcg ctacgctcct
      481 accgtgagac tgcggcgggc gttaccagct caaagatgac gggacacgca agcagtactc
      541 aagtcaggaa gggccggaaa aaggatgcgg cgttgccgtt tttccatagg ctccgccccc
      601 ctgacaagca tcacgaaata tgacgctcaa atcagtggtg gcgaaacccg acaggactat
      661 aaagatccca ggcgtttccc cctggtagct ccctcgtgcg ctctcctgtt cctgcctttc
      721 ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc tcattccacg cctgacactc
      781 agttccgggt aggcagttcg ctccaagctg gactgtatgc acgaaccccc cgttcagtcc
      841 gactaccgcg ccatgtaagg taactatcgt cttgagtcca acccttacag acacaaaaaa
      901 tcgccagtgg cggtagccat tggtaactga tttagaggag gagagtcttg aagtttcggg
      961 tgcgataagg ctacactgaa aggacagttt tggtatctgc gctcctccaa gccagttacc
     1021 ttggttcaaa gagttgctgc tctgagaacc ttcgaaaaac cgccttgctg ggcggttttt
     1081 tcgttttcaa agcaagagat tacgcgcaga aaaaaaggaa ctcaagaaga tcttccccca
     1141 ttaagggcta caaaaagtat gcaacaagcc atccgtatga gaatcgaagc cgataaagca
     1201 gcattagatg ctggttttac tacgaataat tttgtcggtg aactgggcga aaaattagct
     1261 catgccgtat atggaggtcg catacttcct aactcagcta aaagcgcaga catacagaca
     1321 gacgacggca ctttactcca ggtgaaaacg aggaagccga acaaaggaaa acagtttggt
     1381 agcatccgct cttgggattt tgattatctg atagcgataa cgctgggtga taaatacgaa
     1441 gtcatcaggg ccttaaaaat cccagtatct acctgtaaag aactggccga ccatcgggca
     1501 catacgaact ctttcataat aaagccgacc agaaaattac tttcacatgc ggatattgaa
     1561 gatgtgacgg ataaatttca gagtgttgtt ttataggtgg ttttaccccc tacttgaata
     1621 attctgcacg aaatcacgac cgtaaaaaaa ccgccatctt gagtagaagg gcgctattgc
     1681 acaagcatcc ggctttaccg tcttttacca taacatccct tgcacgtacc tacgaactaa
     1741 tcagaacatg caactgatta gaaaatgaaa tctcgactat tgacggccgc tctattttta
     1801 tataaattta gattagttaa ttagattata aatttagagg gctgttcatg attgctgtat
     1861 cacttccaga cgagttacta aaaagactag atgatgctgt agataaaact ggtaaaaaac
     1921 gctcattctt gatacgagaa tcaatatcta tgtatttgaa taacatagaa aacatacatg
     1981 aaaaaaaaga gatcatcctg aacacctcaa aaccgttcta tgaggtcctc attgaagaat
     2041 ttaaaaatga aaaaaaacta ataactgagg ccagaaaaac tgagttcacg atgtttagtg
     2101 ataacggaaa actctatgtc ataaattcga aagggaatac gcgcaggcta gaacccatgt
     2161 atgtgaataa tttctttgaa gagtataaaa agacaggaag tatgagtaca tctagctatc
     2221 aagatataac cttcaactca tcttatcttt tagctgcact acagtacctg atggaaaaag
     2281 aactgattta acataagcac tcattgcccg caccgccccg gacagcttcg ccacgaacgg
     2341 ggcggcagtc agccaaaacc aacaacaacc cacccggtaa accaccccga tcgcgacaga
     2401 taaattttac ccaactgcat agccatgcag ccaggtgaat gacggcagga ttcagcccaa
     2461 aaacagcgtg aaggccccga aaaccggcaa atgcttctcc agccactccc acagttctgc
     2521 cgccagatgc aggcagttaa gtacagcttc aataacctta agcagtttca taattccccc
     2581 tggtataggc cgtgatcttt gcatcacgac gtatttagcg gggaatttcg cagcccttct
     2641 tcttggggtt tgtcacggga cggcatccgt cagcccagcg tcggtccttt gtaggttttc
     2701 tgcggctggc gctgcaactc caggcttttc tgatactcat gctcccgctg ccgctgttct
     2761 accgtactca gctcccggac aagcggcgca gctgctcgct taaactcagc acctgcgtgc
     2821 tcaagtccga cacgcgctcg ttcagcaccg cgttctcccg ttgcataaga ccacacatgc
     2881 tccgccattc cgcgaaggcg ctctcccatt cgtccagcct ttgcgagtag tcctgctgta
     2941 gctgctctaa tgcgctcagc aactgctttt ccagctctgt cattttccgc tactcctgtt
     3001 tcaggcggat ccatgtcgtc ccgtcgtaag ggatggcttc ggtttgcatc cccggcggca
     3061 ggataagaaa cctccggccg tcgctgctct cctgatagcg cactccccac gtccgggcgg
     3121 tcattttcgc cagggtatct tcctgctgac gaagatgcgt gtaattgtcg gttatctgct
     3181 gcccctgcca ccacagaatg ctcccgctcg tgccgatcag tagcgctgac accatcagga
     3241 tggtgagcca tgtccgcccg gcagtccgca gtacgctcag ccggtttttc tccatcgcct
     3301 cgctcatact ctgctcgtgt gcgaggatgg cgtcgctgat tctcctcgcg cttgcgctca
     3361 gttccgctct gacagactgc tcgtgctcgc tgaatgcggc tttcagcatc tcgccggtac
     3421 tctgcttctg cgctttcgat tgctgctcta agtccttcgc cagcgttaaa agactgctca
     3481 tagatggctc ccttcagtcg gatgtttcgc cccccgtccg ggtcggcaat gctgatactg
     3541 cttttcgtgg tgcgcaccac ctcaaaacct gaccgttcca gcgcctcagt gacgtcctga
     3601 cgggttttca gctccccgga cgaagcaagg gtcagcagtc cgcgcgtgat ggcttctgcg
     3661 gcctcctgcg tcgttttcgg cagcccggac ggcgtgatca gcgcccggcg gttttccggt
     3721 gcgttcgggt cgtgcagccc cagtctgccg ttcacgatgg tctgccaggc atcgatacgc
     3781 ggccggtcgg cgcggtcgta gtacggctgg agccgcctgc cggtcagcag ctcggtattg
     3841 gggatcagaa agttcagctc cagacgcccc ttgtcctcgt gctccaccca cagaatgctg
     3901 tactgatctt tatcaagtcc gggcatcaga acccgctcga agctggccat cagtttttcc
     3961 cgctggccgg gaggcagatc ggcctccgca aacgacagga cgccggaggt gtatttcttc
     4021 acgtaaggcg acgcgtcaat cagctcccgg acttcttctg gcttcccctg caggacgctg
     4081 gcgccttcac gctgccgatc tttacccagc agatagtcca ccggcccggc gccaccgccg
     4141 cgaccgcgtg gatgaaattt aacgatcatc gtccgttcct ttctcaagaa cggcgtgccg
     4201 cagccgctca agcccggcat cgatggccat cagcgccgcc acaatctgca ccctgtcatg
     4261 gccggtgccg ccgccggcgt taacacggcg ggctatctgg ttcaggt
//
