LOCUS       NZ_CP122388             1138 bp    DNA     linear   CON 07-JUL-2023
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain KPN-68 plasmid pU7.
ACCESSION   NZ_CP122388
VERSION     NZ_CP122388.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN34122257
            Assembly: GCF_030388445.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Al Marzooq,F. and Daoud,L.
  TITLE     First report of cefiderocol resistance in a pan-drug resistant
            Klebsiella pneumoniae strain from the UAE
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Al Marzooq,F. and Daoud,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2023) Microbiology, UAEU, uaeu, Al Ain, UAE 15551,
            United Arab Emirates
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 08-APR-2023
            Assembly Method :: SPAdes v. 2023-04-08; Unicycler v. 2023-04-08
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 30.0x
            Sequencing Technology :: Oxford Nanopore MinION; MGI DNBseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/05/2023 07:30:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,648
            CDSs (total)                      :: 5,526
            Genes (coding)                    :: 5,377
            CDSs (with protein)               :: 5,377
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 70 of 149
            Pseudo Genes (incomplete)         :: 74 of 149
            Pseudo Genes (internal stop)      :: 26 of 149
            Pseudo Genes (multiple problems)  :: 18 of 149
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP122388.1.
            Bacteria and source DNA available from Dr. Farah Al-Marzooq lab-
            CMHS-UAEU-Al Ain, UAE.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPN-68"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pU7"
                     /geo_loc_name="United Arab Emirates: Al Ain"
                     /lat_lon="24.20168402 N 55.67599108 E"
                     /collection_date="2021-03-14"
                     /collected_by="UAEU"
     gene            complement(90..1070)
                     /locus_tag="QBK98_RS28240"
                     /old_locus_tag="QBK98_28240"
     CDS             complement(90..1070)
                     /locus_tag="QBK98_RS28240"
                     /old_locus_tag="QBK98_28240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISKpn26 family transposase"
                     /protein_id="WP_000019473.1"
                     /translation="MSHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMVEVIEPFY
                     PKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDRTT
                     IMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQRDPE
                     MHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAG
                     YQGAPQREELAEVDVDWLIAERPGKVKTLKQHPRKNKTAINIEYMKASIRARVEHPFRI
                     IKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRVDQMIRQWERSQ"
                     /name="IS5-like element ISKpn26 family transposase"
ORIGIN
        1 cggatctggg ccgatttttt tcccgcaaac acatcgaatc agcctattta ggctattttt
       61 tccaccattt ctggcgttat ttccggtttt tactgagatc tctcccactg acgtatcatt
      121 tggtccaccc gaaacaggtt ggccagggtg aataacatcg ccagttggtt atcgtttttc
      181 agcagccctt tgtatctggc tttcacgaag ccgaactgcc gcttgatgat gcgaaacggg
      241 tgctccaccc tggcacggat gctggctttc atgtattcga tgttgatggc cgttttgttc
      301 ttgcgcggat gctgcttcaa ggtttttacc ttgccgggac gctcggcgat cagccagtcc
      361 acatccacct cggccagctc ctcgcgctgt ggcgctcctt ggtagccggc atcggctgag
      421 acaaattgct cctctccatg aagcagatta cccagctgat tgaggtcatg ctcgttggcc
      481 gcggtggtga ctaggctgtg ggtcaggcca ctcttggcat cgacaccaat gtgggccttc
      541 atgccaaagt gccactgatt gcctttcttg gtctgatgca tctccggatc gcgttgctgc
      601 tctttgttct tggtagagct gggtgcctca atgatggtgg catccaccaa agtgccttgg
      661 gtcatcatga cgcctgcttc ggccagccag cgattgatgg tcttgaacaa ttgacgggcc
      721 agttgatgct gctcgagcag gtggcggaaa ttcatgatgg tggtgcgatc cggcagggcg
      781 ctatccaggg ataatcgggc aaacaggcgc atggaggcga tttcgtacag ggcatcttcc
      841 atggcaccgt cgctcaggtt gtaccaatgc tgcatgcagt gaatacgcag catggtctcc
      901 agcggatagg gccgtcggcc attgcccgcc ttgggataaa acggctcgat gacttccacc
      961 atgttttgcc atggcagaat ctgctccatg cgggagagga aaatctcttt tcgggtctga
     1021 cggcgcttag tgctgaattc actatcggcg aaggtgagtt gatggctcat gatgtccctc
     1081 tgggatgcgc tccggatgaa tatgatgatc tcatatcagg aacttgttcg caccttcc
//
