LOCUS       NZ_CP121786             4135 bp    DNA     circular CON 05-SEP-2023
DEFINITION  Acinetobacter johnsonii strain AYTCM plasmid pAYTCM-10, complete
            sequence.
ACCESSION   NZ_CP121786
VERSION     NZ_CP121786.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN34117139
            Assembly: GCF_030867405.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter johnsonii
  ORGANISM  Acinetobacter johnsonii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Tian,C., Wang,S., Fan,X. and Xia,D.
  TITLE     Acinetobacter johnsonii isolate AYTCM encoding NDM-1, OXA-58 and
            PER-1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Tian,C., Wang,S., Fan,X. and Xia,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) Department of Pharmacy, Department of
            Pharmacy, Shaoxing Hospital of Traditional Chinese Medicine
            Affiliated to Zhejiang Chi, Renminzhong Road 641, Yuecheng district,
            Shaoxing, Zhejiang 312000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/25/2023 18:02:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,979
            CDSs (total)                      :: 3,866
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 113
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 57 of 142
            Pseudo Genes (incomplete)         :: 88 of 142
            Pseudo Genes (internal stop)      :: 30 of 142
            Pseudo Genes (multiple problems)  :: 29 of 142
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP121786.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Acinetobacter johnsonii"
                     /mol_type="genomic DNA"
                     /strain="AYTCM"
                     /isolation_source="patient"
                     /db_xref="taxon:40214"
                     /plasmid="pAYTCM-10"
                     /geo_loc_name="China: Zhejiang"
                     /collection_date="2018-03-19"
     gene            399..800
                     /locus_tag="QBJ73_RS19875"
                     /old_locus_tag="QBJ73_19880"
     CDS             399..800
                     /locus_tag="QBJ73_RS19875"
                     /old_locus_tag="QBJ73_19880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004706792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_050041694.1"
                     /translation="MEIDSVTKQKAVRERTFKVRLSESEYQLLDANNPFGSIAKLLRQS
                     AVEKATQIHNSKADDDQKIKAKNVVESSYTKTERSLILELGRIGNNVNQIAKAINTDIA
                     GTGTFDKVKLLHLLISIDQQLRELRPDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            790..2604
                     /locus_tag="QBJ73_RS19880"
                     /old_locus_tag="QBJ73_19885"
     CDS             790..2604
                     /locus_tag="QBJ73_RS19880"
                     /old_locus_tag="QBJ73_19885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004706791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_176453806.1"
                     /translation="MIVDFFRHGTGLSKGCLDYLLGEDRVREHAQVLSGDVELTAQLID
                     SSPFAKKYTSGCLSFYEHDLSDQDKQQIMQNFEQCLFPGLDQDQYKVLWVQHQDKVNQV
                     TGETRLELNFVIPNVELSTGKRLQPFYAPVDLDRVDLFKQITNAEHRLYDPDDPNNQQL
                     LINKKNLPKDVRDFKAQLHQRIYRAVANGEIADRQKLVEWLESNDVKVTRQTQKSISIE
                     NPYEGATRPIRLNGEVYEQGFRATGEYRQEVQQRIATYRGTTSERYRANVTDYQRQLKH
                     KSQYHSDRYPTVRREISAEPTEQLTADRKAARPVPSLATVQIEPFKANERADTDRRTAS
                     SEQKPTETAYYFEYGTDFGRCYFDYSHYCAQFYGEKQVQRDSDAQYDDQRETNASGSPE
                     PIGGQFDVQHMQTAKQQSPTLMYSDQQECRRMAERLHDSDGVLNDDRIRNTVIENHRRT
                     TAAITATTSAIAAATAEFRRDAKENYPSLISTIKGNGERTERLSANTKVIIDNTDAISR
                     IREKYGNVYRADQWQSQRDEPNYNELRGRTGENLNRTAVSLVRSSQSAAEFARNVRTIE
                     ANIIQMKERKMEMEQPQPKQDRGMSWGM"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2650..3438)
                     /locus_tag="QBJ73_RS19885"
                     /old_locus_tag="QBJ73_19890"
     CDS             complement(2650..3438)
                     /locus_tag="QBJ73_RS19885"
                     /old_locus_tag="QBJ73_19890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004758588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-II family aminoglycoside
                     nucleotidyltransferase AadA27"
                     /protein_id="WP_050041693.1"
                     /translation="MSETLQLEQLTESLQQLLGESLFAIYLYGSAVDGGLGPESDLDVL
                     VVVNQALTLHQRQQLAETLLKISYPIGAAQRALEVTIVLKEQILSGSYPLSYELQFGEW
                     LREELNQGALLRAHTDPDLSILLKKAQMHHRSLLGPSLTQWSTAIPEQHLWQAMADTYP
                     SIVAHWDEDADERNQILALCRIYFSLITNEIVPKDQAAHWVIAQLPSLHQPILQRMIQE
                     YKGEIRKQNWQQQHQALGPVVDFLSSKIDEQFNKKSSLIK"
                     /name="ANT(3'')-II family aminoglycoside
                     nucleotidyltransferase AadA27"
     ARG             complement(2654..3438)
                     /name="aadA27"
                     /gene="aadA27"
                     /gene_name="ANT(3'')-II family aminoglycoside
                     nucleotidyltransferase AadA27"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          791..2601
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atcaggttaa atcggcaata cttaagggaa gtgtcatttt ttgaatgaca gcccttaggg
       61 agagtattgc cgaaactctc tcaacgtcca ccatctgcct gagcgaagcg aaggctgtgg
      121 tggcggtgag aaagagttga gcgtctttag ttttacaaga ttaatgccaa tactttctag
      181 agtttatata ccgagtcatt tgatttaata atttatagaa caaatttgtc cgtaattgat
      241 aagcaaggat acacagttac ccctatttca cgatgttcaa tagtgctaag ctgtgacctt
      301 gatcaggggc ttcacccccg ataccccgaa aaccggctta aagaaaaatc aaagattttc
      361 ccttaaaccg gtttcatacc gagaattgga ttgatcaaat ggaaatagat tcagtaacta
      421 aacagaaagc ggttcgggaa cggacgttta aagtcagact ttcagaatca gagtaccaat
      481 tgcttgatgc taataatcct tttggttcaa tcgcaaagtt gctacgtcaa tcggcagtag
      541 agaaagcaac acagattcat aattcaaaag cagatgatga ccaaaaaatt aaagcaaaaa
      601 acgttgttga atcttcatat accaagacag aacgttcttt aattttagag ttaggtcgca
      661 taggtaataa cgtcaatcag attgccaagg ctattaatac ggatattgca gggacaggta
      721 cttttgacaa agtgaagtta cttcatttgt tgatttccat agatcagcaa cttcgggagt
      781 tacgtccaga tgatcgttga tttttttaga catggcacag gtttatccaa aggttgtcta
      841 gattatttac ttggtgaaga tcgagtgcga gaacatgcac aggttttaag tggtgatgtg
      901 gagctgacag cacaactcat tgatagcagc ccttttgcca agaaatatac cagcggttgt
      961 ttatcctttt acgagcatga tttatctgac caggataaac agcagatcat gcagaatttt
     1021 gagcagtgtc tttttcctgg ccttgatcaa gatcagtata aagttctatg ggtacagcac
     1081 caagataagg tgaaccaagt tactggagag actcggcttg agctgaattt tgtgattcct
     1141 aatgtggaac tttcgacagg caaacggcta cagccatttt atgcaccggt agatctcgat
     1201 cgagtagatc tgtttaagca gattaccaat gcggaacata gactgtatga ccctgatgac
     1261 ccgaacaatc agcagctttt aatcaataag aagaacctcc caaaggacgt tcgggacttt
     1321 aaagcgcaat tacatcaacg tatctatcga gccgttgcca atggtgaaat tgccgatcgg
     1381 caaaaattgg ttgaatggct tgagtccaat gacgtcaaag tcacccgaca gacccaaaaa
     1441 tcaatatcaa ttgaaaatcc atatgagggc gcaacacgtc ctatccgttt gaatggagag
     1501 gtttatgagc aaggctttag agctactggc gaatatcgac aagaagtaca acagcgaatt
     1561 gcaacgtacc gaggaacaac ttctgagcgg tatcgagcaa acgtcacaga ttatcaaaga
     1621 caacttaagc acaagagcca atatcacagc gaccgctatc caacagtcag acgagaaatt
     1681 agcgcagaac ctacagaaca gctcacagca gatcgaaaag cagctcgacc agtaccaagc
     1741 cttgctacag tccaaattga gccgtttaaa gcaaatgaac gagcagatac agacagaaga
     1801 acagcaagtt cagaacaaaa gcctactgaa acagcttact actttgaata tggcactgat
     1861 tttgggcgct gttattttga ttattctcat tattgcgctc agttttatgg ggaaaagcaa
     1921 gtacagcgag attcagacgc tcaatatgac gatcaacgag aaacaaatgc gagtggatca
     1981 cctgaaccga tcggggggca gtttgacgtt cagcacatgc agacagcaaa acagcaaagc
     2041 ccaacgctta tgtattcaga tcaacaagaa tgcaggcgaa tggcagaacg gttacatgat
     2101 tccgatgggg tattaaacga tgaccgaatt agaaacacag ttattgaaaa tcatcgaaga
     2161 acaacagcag caattacagc aacaacaagc gcaattgcag cagcaacagc agagtttaga
     2221 cgtgatgcaa aagaaaatta tccaagcctt atcagcacaa tcaaaggcaa tggagagcgt
     2281 acagaaaggc ttagtgcaaa cacaaaagtc attatcgaca acacagacgc aatttcacga
     2341 attagagaga agtacggcaa cgtataccga gcagatcaat ggcaaagtca gagagatgag
     2401 ccaaattaca acgaactacg aggacgtact ggagaaaatc tcaaccgtac agcagtcagt
     2461 ctcgtcagaa gtagccaaag cgcagcagaa tttgctcgaa acgtccgaac tattgaagcg
     2521 aatatcatcc agatgaagga acggaaaatg gaaatggaac agccccaacc taagcaagat
     2581 cgagggatga gttgggggat gtagggctta tagaagcccg tcagctcaaa atgcttggtt
     2641 aaaacactat tacttgatta ggctactctt cttattaaat tgttcatcaa tttttgaact
     2701 aaggaagtca acaacagggc ctaaagcctg atgctgttgt tgccagtttt gtttgcgtat
     2761 ctcaccttta tattcttgga tcatgcgctg caaaatgggt tgatgcagcg acggtaactg
     2821 agctatcacc cagtgtgcgg cttggtcttt aggcacaatc tcattcgtta tcaagctaaa
     2881 ataaatacgg cataaggcca aaatctgatt acgttcatcg gcatcctcat cccaatgtgc
     2941 cacaatagag ggataggtgt ctgccattgc ctgccagagg tgctgttcag gaattgccgt
     3001 tgaccactgt gtcaaacttg gccccaacaa actacgatga tgcatttgtg ctttcttcag
     3061 caaaatactc agatcagggt ctgtatgtgc gcggagcaaa gcaccttggt ttaactcctc
     3121 ccgtaaccac tccccaaatt gtagttcgta gctgagtgga taactgcctg agagaatttg
     3181 ctctttaagt acgatggtga cttcaagtgc acgctgtgca gccccaattg gatacgaaat
     3241 ttttaataag gtttctgcca gttgctgtct ttgatggagt gtcagtgctt gattcaccac
     3301 aaccaaaaca tccagatcac tttctggacc tagcccgcca tcaacagctg aaccatacag
     3361 ataaatggca aagagggatt cacccaaaag ctgctgtaaa gattctgtta actgttcaag
     3421 ttgcaaggtt tcggacatgc ttgacctctt tttcgggcat gagttaggag taggagaaac
     3481 aaataaaaat cttgttaaaa aatttgaaat atataaaaaa agagtccttg caacaattct
     3541 aatgaaaagt tgtagttaaa acctaaagaa aaacccctct gagtcttgca acttagaggg
     3601 gttttgtctt aaatttggtg gtacgcagtt atttgatgtt aagagcatca agtaacgaag
     3661 atcagcagag ttagacgctc gtactgattt cacctttcgg cttgtaccgc ttttcagatt
     3721 ctatcaaatt gataaaaaat ggcaagcatt gtatcagtaa aaagtctttc gttacttgat
     3781 tctcgttcat ccaataagca atacatagac tgtattcgag ccttatcagc ttctgttcgc
     3841 cttttttaaa ggaagatatg cggtgcatgt ggagggaata agtgtcactg gtgcatcgta
     3901 gcgatagtga agatcgagag attggagcta aaccgttaca ggggaatccc acacaagcgg
     3961 agcgttgaga gtagttgtat aggttggtta agttgcatgt tggagaaatc ctcgcagaga
     4021 taccaccata cttcgacagg gagcgcgtgg gggaaggtac aaaactttta gtccttgtct
     4081 accatgtatg taaagcatgg tgcggatacg accgatgcgt ggctccgtca gccta
//
