LOCUS       NZ_CP121785             6147 bp    DNA     circular CON 05-SEP-2023
DEFINITION  Acinetobacter johnsonii strain AYTCM plasmid pAYTCM-9, complete
            sequence.
ACCESSION   NZ_CP121785
VERSION     NZ_CP121785.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN34117139
            Assembly: GCF_030867405.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter johnsonii
  ORGANISM  Acinetobacter johnsonii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   Tian,C., Wang,S., Fan,X. and Xia,D.
  TITLE     Acinetobacter johnsonii isolate AYTCM encoding NDM-1, OXA-58 and
            PER-1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6147)
  AUTHORS   Tian,C., Wang,S., Fan,X. and Xia,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) Department of Pharmacy, Department of
            Pharmacy, Shaoxing Hospital of Traditional Chinese Medicine
            Affiliated to Zhejiang Chi, Renminzhong Road 641, Yuecheng district,
            Shaoxing, Zhejiang 312000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/25/2023 18:02:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,979
            CDSs (total)                      :: 3,866
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 113
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 57 of 142
            Pseudo Genes (incomplete)         :: 88 of 142
            Pseudo Genes (internal stop)      :: 30 of 142
            Pseudo Genes (multiple problems)  :: 29 of 142
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP121785.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6147
                     /organism="Acinetobacter johnsonii"
                     /mol_type="genomic DNA"
                     /strain="AYTCM"
                     /isolation_source="patient"
                     /db_xref="taxon:40214"
                     /plasmid="pAYTCM-9"
                     /geo_loc_name="China: Zhejiang"
                     /collection_date="2018-03-19"
     gene            join(5500..6147,1..285)
                     /gene="repM"
                     /locus_tag="QBJ73_RS19845"
                     /old_locus_tag="QBJ73_19850"
     CDS             join(5500..6147,1..285)
                     /gene="repM"
                     /locus_tag="QBJ73_RS19845"
                     /old_locus_tag="QBJ73_19850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004641836.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_120376160.1"
                     /translation="MKNGLVVKDNALMNASYNLETSEQRLILLAIMNARETGQGITADS
                     KLEIHASDYAKHFNVSTDAAYKVLKDAVNNLFERKFSYVSEYKKTGKTGIVRSRWVSRV
                     FYVDTLAILEITFAPDVVPLVTRLEEHFTSYQLKQVSQLTGKYAIRLYELLIAWREVGK
                     TPQIELSEFRNRVGVEDNEYQRMELFKRRVLEPSIKQINEHTDITVTYEQHKKGRAISG
                     FSFKFKQKQQAKKIESKRDPNTPDFFIKMTDSQRHLFANKMSEMPEMGKYSQGTESYQQ
                     FAIRIADMLLQPEKFRELYPILEKAGFKE"
                     /name="repM"
     gene            282..470
                     /locus_tag="QBJ73_RS19850"
                     /old_locus_tag="QBJ73_19855"
     CDS             282..470
                     /locus_tag="QBJ73_RS19850"
                     /old_locus_tag="QBJ73_19855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010591710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_270832363.1"
                     /translation="MIEKEITKFEKEILLQDKISQLENELKEFSDLQKKAYSDRLQKSI
                     VGLENRIQRIKKSLYTN"
                     /name="hypothetical protein"
     gene            620..1753
                     /gene="mobV"
                     /locus_tag="QBJ73_RS19855"
                     /old_locus_tag="QBJ73_19860"
     CDS             620..1753
                     /gene="mobV"
                     /locus_tag="QBJ73_RS19855"
                     /old_locus_tag="QBJ73_19860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005080191.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_171530389.1"
                     /translation="MSFAILRIQKLKSFADVGGSLSHNYRNRETLNADSDRTHLNEHEL
                     DTNEKCMTAIRDRIPEKRRKDAVLCIEHLVTASPEWDGWGTEKETAFFEQSKKWLENKY
                     GKNNVVSTTIHRDETTPHLVAYVVPVDEATGRLNAKKYIGGSRHTLSQMQTDFAVEVKD
                     LGLDRGVQGSKAKHTSIKEYYEKLNNYENEPGIEKGLTYEIPEPEFLESKNTYGERVAN
                     AMIDQIFDQIAPRFDRANALASQTEKLKKELSDTKKTLDEVQQRAKPYLDIINEYNHPN
                     LEKEFNKQVAKLKDNFDSALEHHRFLKRQEEQERFNQQRELRNQLHLEQEQKKQLAEQE
                     RQEKERLALLRRQELEKQRKNEPKKPDNGNDNDYSPS"
                     /name="mobV"
     gene            2406..3344
                     /locus_tag="QBJ73_RS19860"
                     /old_locus_tag="QBJ73_19865"
     CDS             2406..3344
                     /locus_tag="QBJ73_RS19860"
                     /old_locus_tag="QBJ73_19865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005080187.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_005080187.1"
                     /translation="MDLDVKKHYPKDWIVLQNRVVECFRGMSLDEKRLFIMATPLARTT
                     KISSNDPIFISSSDFSKECGIDLSTAYTALELASERLFTRFFGYTNAEGDRVKMRWLNK
                     VIYKAGQGGSELYFTDEVLLLLREFDALNPYTKYKKEVVLRLKKDYSLDFYHLAKKHQT
                     MGGFQISLDELFQQLGLPESYQDLSNLKKRVIKPSLDEITANTDIDLSYENVKRGRSVV
                     GFKFTVKEKPKPKVIETGRDPNTPDLFHKMTESQLDTFSSKLSELPEVQKMAHAGEDMK
                     PFIARIRSMLKDSEKQKTLLPHLAKLGFKSK"
                     /name="replication initiation protein"
     gene            3341..3532
                     /locus_tag="QBJ73_RS19865"
                     /old_locus_tag="QBJ73_19870"
     CDS             3341..3532
                     /locus_tag="QBJ73_RS19865"
                     /old_locus_tag="QBJ73_19870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005080189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_180181480.1"
                     /translation="MMFEKKITQFEREVLLQEKIEQLENELKEFSDLQKKAYSERLQKS
                     MVGLENRIYRIKKMLYTT"
                     /name="hypothetical protein"
     gene            3684..4928
                     /gene="mobV"
                     /locus_tag="QBJ73_RS19870"
                     /old_locus_tag="QBJ73_19875"
     CDS             3684..4928
                     /gene="mobV"
                     /locus_tag="QBJ73_RS19870"
                     /old_locus_tag="QBJ73_19875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005080191.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_308469725.1"
                     /translation="MSFAILRLQKLKSFADVGGSLSHNYRNRETLNADSDRTHLNDHDL
                     DTNEKCMSAIRDRIPEKRRKDAVLCIEHLITASPDWDGWGTEKETAFFEQSKKWLENKY
                     GKNNVVSTTIHRDETTPHLVAYVVPVDEETGRLNAKKFIGGSRHTLSQMQTDFAVQVKD
                     LGLERGIEGSKAKHTSIREYYNDVNNYEPEPNIVLQTPEPNGMFESKTQYAERIIESVI
                     EQIEPKLKELNLLANQTRSAKKDAQNALKTASEARKALSDLQEHVKPYLDALDGLDQTW
                     KEYFDKTLRAAKEQVLEKRKQLQVEKENEELSLKLKKEERIFQMEWNNTFAGFNKEQIK
                     NYEYQLECLEQDYPVEFYRQIQIKSLQENLILNPNYKRYSKNEFHQEPEQKVQPTQKND
                     QEKRSGNDYDSPPPF"
                     /name="mobV"
     Typing          2407..3344
                     /name="rep_cluster_1223"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.823"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          594..1762
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.487"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3685..4925
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.961"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggtcgagcta tttcaggctt ttcattcaaa tttaagcaga agcaacaagc aaagaaaatc
       61 gaatctaaac gagatcctaa tacacccgat ttctttatca agatgactga ctcacaacgc
      121 catttatttg ctaataaaat gtctgaaatg cccgaaatgg ggaaatactc acaaggtaca
      181 gaaagctacc aacaatttgc tattcgcatc gccgacatgc ttttacaacc tgaaaaattc
      241 agagagcttt atccaatttt agaaaaagcc ggattcaaag aatgattgaa aaagagataa
      301 caaaatttga gaaagagatt ttgctacaag acaaaatctc tcagctcgaa aatgaactaa
      361 aagaattttc tgatcttcaa aaaaaagcat atagcgatcg tctgcagaaa agcattgtcg
      421 gtttagaaaa ccgaattcaa cgcatcaaaa aatcgcttta tacaaactga aagtttgtcg
      481 gggtcgaggg gaggattccc cttgctcatg aacttttaag accccataag gggttttaaa
      541 agtggagtga gtccacttcg ctatcgcaaa gctcaaaaag ctctgctatc gtgtgttttt
      601 tcggtatcgg atttctcaca tgtcatttgc tattttgcgt attcaaaaat tgaagtcgtt
      661 tgcagacgtt ggtggcagtc tttcgcataa ttatcgcaat cgagaaacgt taaatgccga
      721 ttctgaccgc acacatttga atgagcatga gttagataca aacgaaaaat gtatgaccgc
      781 tattcgtgat cgtattcctg aaaaaagacg caaagatgcg gttttgtgta ttgaacatct
      841 tgtgactgca tcaccagaat gggacggctg gggaactgaa aaagaaactg cattttttga
      901 gcagtcgaaa aaatggctcg aaaataaata cggtaaaaat aatgtcgtca gcactacgat
      961 tcatagagat gaaacaaccc ctcatttggt cgcttacgtt gttccagtcg atgaagctac
     1021 gggccgttta aacgcaaaaa aatatatcgg tggatctcga catacacttt cacagatgca
     1081 gactgatttt gcagttgaag taaaagattt aggattagat cggggcgtac aggggagcaa
     1141 agcaaaacat acgtctatca aagaatatta tgagaaattg aacaattacg aaaatgaacc
     1201 tggtattgaa aaaggtctca cttacgaaat acctgaacct gaatttcttg agtcaaaaaa
     1261 tacctacggt gagagagtcg caaacgctat gattgatcag atatttgatc aaattgcacc
     1321 acgatttgac cgagcgaatg cactggctag tcaaactgaa aaattaaaaa aagaactgtc
     1381 tgatacaaaa aaaacacttg atgaggttca gcaacgagca aagccatatt tagacataat
     1441 taacgagtat aaccatccaa atcttgagaa agaatttaat aagcaagttg ctaaattaaa
     1501 agataatttt gattcagcac ttgaacatca tagattccta aaaagacagg aagaacaaga
     1561 aagatttaac caacaacgtg aacttcgcaa tcaattacac ttagaacaag aacagaaaaa
     1621 acaactggct gaacaagaaa ggcaggaaaa agaacgtttg gcacttttaa gacgtcaaga
     1681 attagaaaaa cagcgaaaaa atgagcctaa aaaacctgat aacggcaatg ataacgacta
     1741 ctcaccctca taatacccat ttaaacgcaa aaaaatgggg tttttagcca tgtttggtgt
     1801 tttatgcatg aaagtatagt tgagataata aaaacctctt agaacgcaaa ttagagcgtt
     1861 ttagcgagtg tctacgagcg acacaatgaa aatttgctta ttccccctct gaaaaaccgc
     1921 ttttgctttt taaaacagat gaatcgcaga tgtagcaatg ctttcaacgc tatatctata
     1981 cgtttatcga cccaagtagt tagcgagtgt ctacgagcga agtattgatg cttttgctct
     2041 tcaaaaagca tgagcatagc gaatgcatta ttaacgcttt tgcttttgct tttgctcttg
     2101 aaaagacacg agcgaagcga gtgccatagc ctttgatttt gcctttgatt ctctttttta
     2161 gcttttaaaa agcttttaaa gacttttcgc ccctctacag accttactag acatagcttt
     2221 cagactttaa aaaagtacaa acgccaagta ttaaagtaca aacgccaagt taaaagcact
     2281 taaaaaagta caaacgccaa gttaaaaaag tacaaacgcc aagtattaaa gtacacaatt
     2341 aaactttgaa cttttattga ttaaaaggta ttgtcttaaa cgatgtactt tatttggaaa
     2401 atactatgga tttggatgtt aaaaaacact atcccaaaga ctggatagtt ttacaaaata
     2461 gagtggttga atgctttaga ggtatgtccc ttgatgaaaa gcgtttattt atcatggcaa
     2521 cccctttggc aagaaccaca aaaatatcaa gcaatgatcc tatttttata tcttcgagtg
     2581 acttttcaaa ggagtgtggc attgacttat caacggctta tacagcttta gaattggcat
     2641 ctgaacgcct atttactcgg ttctttggtt acaccaatgc tgagggtgac agggtaaaaa
     2701 tgcgttggct aaacaaggtg atttataagg caggacaagg cggttcagaa ctatatttta
     2761 ccgatgaagt actgctatta cttagagagt ttgacgccct aaacccttat accaagtata
     2821 aaaaagaggt cgttttacgg cttaaaaaag actactcgct agacttctat cacttggcta
     2881 agaaacacca aacaatgggc ggttttcaaa tcagcctaga cgaattattt cagcagttgg
     2941 gcttgcctga atcctatcaa gacctaagca acctaaaaaa acgtgtaatt aagccatcac
     3001 tggacgagat cacagccaac accgacatag acctaagcta tgagaatgtg aaacgtgggc
     3061 gttccgtggt tggctttaag ttcactgtca aagaaaaacc aaagcccaaa gttatagaaa
     3121 caggacgaga tccaaataca cctgacctat tccacaaaat gacagaatct caacttgata
     3181 cgtttagcag taaactttca gaactccctg aagtgcaaaa gatggcacat gcaggcgaag
     3241 atatgaaacc atttattgct cgtattcgtt cgatgctcaa agactctgaa aaacaaaaaa
     3301 cgttgcttcc ccatctagca aaattaggat ttaaatcgaa atgatgtttg agaaaaaaat
     3361 tactcagttt gaaagggaag ttttgctaca agaaaaaatt gagcagctcg aaaacgagct
     3421 taaagaattt tctgatcttc aaaaaaaagc atatagcgaa cggcttcaaa aaagtatggt
     3481 gggtttagaa aatagaatct atcgaatcaa gaaaatgctt tatacaacct gaaaggttgt
     3541 caggggttaa ggggaggatt ccccttactc atgaactttc aatgtacctt taggtgcttt
     3601 gaaagtggag tgagtccact ttcgctatcg caaagctcaa aaccatctgc tatcgtgtgc
     3661 tttttgcggt attggatctc tcaatgtcat ttgctatttt gcgccttcag aaattgaaat
     3721 cgtttgccga tgtcgggggc agtctttctc ataattatcg caatcgtgaa acgctcaatg
     3781 ccgattctga tcgcactcat ttaaatgatc atgacttaga cacaaacgaa aaatgtatgt
     3841 ctgctattcg tgatcgtatt cctgagaagc ggcggaaaga tgctgttttg tgcattgaac
     3901 atttgatcac ggcaagcccc gactgggacg gctggggcac agaaaaagaa actgcatttt
     3961 ttgagcagtc gaaaaaatgg ctcgaaaata aatacggtaa aaacaatgtt gtcagcacta
     4021 caattcatag agatgaaaca acgccccatt tagttgctta tgtcgttcca gtggacgaag
     4081 aaacaggacg attgaatgct aaaaaattta ttggcggatc tcgacataca ctttcacaga
     4141 tgcagactga ttttgcagtt caggtaaagg atttagggct tgaacgagga attgagggaa
     4201 gtaaggcaaa acatacgtct attcgtgagt actacaacga tgtaaacaac tatgaacctg
     4261 aacccaatat tgtacttcaa acacctgagc ccaatggaat gtttgagagt aaaacacaat
     4321 atgctgaaag aattattgaa tctgtgattg aacaaatcga accaaaatta aaagaattaa
     4381 atttattagc aaatcaaact agatcagcaa aaaaagatgc acaaaatgca cttaaaacag
     4441 cctcagaggc tcgtaaagca ctttcagatt tacaagaaca tgtaaaaccc tatctggacg
     4501 cactggacgg cctggaccaa acatggaaag aatattttga taaaactttg cgagcagcta
     4561 aagaacaagt gctagaaaaa aggaagcaat tgcaagttga aaaagaaaat gaagaactat
     4621 cactaaaact aaaaaaagaa gaaagaatat ttcaaatgga atggaacaat acatttgctg
     4681 gtttcaataa agaacaaatc aagaattatg aatatcaact tgagtgtctt gaacaagatt
     4741 atcctgttga attttataga caaattcaaa ttaaatcatt gcaagaaaat cttattttga
     4801 atccaaatta caagagatac agtaaaaatg agttccatca agaaccagaa caaaaagtac
     4861 aaccgacaca aaaaaatgat caagaaaaac gatctggcaa tgattacgat tcaccaccac
     4921 cattttaaaa tgctctagaa cgaaaaaaaa cggggttttt agccctattt ggtcttttct
     4981 gcataaaagt ataattgaaa taaaaaatgc tcttagaacg caaatgagag catttagcga
     5041 gtgtctacga gcgacacaaa aaaattcgct tattacccct ctgaaaaacc gcttttgctc
     5101 ttttttgctt gctgcaggaa tttattagcg agtgtctacg agcgaagtat tgatactttt
     5161 gctcttaaaa aagcatgagc atagcgaatg cattatcaat gcttttgact ttgcttttgc
     5221 tcttgaaacg acacgagcaa agcgagtgcc atagcctttg atttttgatg ctcttgggct
     5281 tttaatatct tttaaagctt ttaaatgctt ttaagcgagc cgaaacgatt gctacacaag
     5341 gcttgtagag gacatatagc accatttcct cttgtataaa gcaccatttc ctcttgtata
     5401 aagcaccatt tcctcttgta taaagcacca tttcctcttg acttaacacc attagcattt
     5461 taaggtgcta tcaaaccaag atacatctat ggtgctaaat tgaaaaatgg attagtagtg
     5521 aaagacaatg cgttaatgaa tgccagttat aacttagaaa cgtctgaaca gcgtttaata
     5581 ttgcttgcaa ttatgaatgc tagagaaaca gggcaaggaa ttaccgcaga cagcaagtta
     5641 gaaatacatg ctagtgatta cgcaaaacac tttaatgtgt ctacagatgc tgcatacaaa
     5701 gtattaaaag atgctgtaaa taatctgttt gaacgtaagt ttagctatgt ttcggaatat
     5761 aaaaagactg gcaaaacagg cattgtgcgc tcacgttggg ttagtcgtgt tttctatgtt
     5821 gatacgttag cgattttaga aatcactttt gcccctgacg ttgtaccact tgttactcgg
     5881 cttgaggaac actttacaag ttatcaactt aaacaggtat cacagcttac aggtaagtat
     5941 gcaattcgat tatacgaact actaattgca tggcgtgaag tgggtaaaac tccacaaatt
     6001 gaactatcag aattcagaaa tagagttggt gttgaggata atgaatatca acgtatggag
     6061 ctgtttaaaa ggcgtgtctt agaaccgtct atcaagcaga tcaatgaaca taccgatatc
     6121 actgtgacct atgaacaaca taaaaaa
//
