LOCUS       NZ_CP121784             6289 bp    DNA     circular CON 05-SEP-2023
DEFINITION  Acinetobacter johnsonii strain AYTCM plasmid pAYTCM-8, complete
            sequence.
ACCESSION   NZ_CP121784
VERSION     NZ_CP121784.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN34117139
            Assembly: GCF_030867405.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter johnsonii
  ORGANISM  Acinetobacter johnsonii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 6289)
  AUTHORS   Tian,C., Wang,S., Fan,X. and Xia,D.
  TITLE     Acinetobacter johnsonii isolate AYTCM encoding NDM-1, OXA-58 and
            PER-1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6289)
  AUTHORS   Tian,C., Wang,S., Fan,X. and Xia,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) Department of Pharmacy, Department of
            Pharmacy, Shaoxing Hospital of Traditional Chinese Medicine
            Affiliated to Zhejiang Chi, Renminzhong Road 641, Yuecheng district,
            Shaoxing, Zhejiang 312000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/25/2023 18:02:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,979
            CDSs (total)                      :: 3,866
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 113
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 57 of 142
            Pseudo Genes (incomplete)         :: 88 of 142
            Pseudo Genes (internal stop)      :: 30 of 142
            Pseudo Genes (multiple problems)  :: 29 of 142
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP121784.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6289
                     /organism="Acinetobacter johnsonii"
                     /mol_type="genomic DNA"
                     /strain="AYTCM"
                     /isolation_source="patient"
                     /db_xref="taxon:40214"
                     /plasmid="pAYTCM-8"
                     /geo_loc_name="China: Zhejiang"
                     /collection_date="2018-03-19"
     gene            113..1285
                     /gene="blaMCA"
                     /locus_tag="QBJ73_RS19810"
                     /old_locus_tag="QBJ73_19815"
     ARG             114..1282
                     /name="blaMCA"
                     /gene="blaMCA"
                     /gene_name="MCA family class C beta-lactamase"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="99.23"
                     /coverage="100.0"
     gene            complement(1470..1757)
                     /locus_tag="QBJ73_RS19815"
                     /old_locus_tag="QBJ73_19820"
     CDS             complement(1470..1757)
                     /locus_tag="QBJ73_RS19815"
                     /old_locus_tag="QBJ73_19820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004870774.1"
                     /translation="MRTKAELDAMSHQELKDYEQSLLALWTPRMAIESDIERLSTHHSE
                     LLEVFNQLKNPDAPKNSRLKDSILSLKYKIESLEGKLSDLIQDNRLNSAD"
                     /name="hypothetical protein"
     gene            complement(1754..2695)
                     /locus_tag="QBJ73_RS19820"
                     /old_locus_tag="QBJ73_19825"
     CDS             complement(1754..2695)
                     /locus_tag="QBJ73_RS19820"
                     /old_locus_tag="QBJ73_19825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004729701.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_020846814.1"
                     /translation="MDENKKTYPLSWVVMQNNVQECFKSMNIDEKRMLILASPIARTTD
                     ATEKDRIMITAEEFARECGIKTHSAYTQLELASKNLLKRNFSYNNERGKKVLSNWVIDC
                     TYEDGGIAIRFPEIVLLMLKEFDKLNPYTKYKKDIVLSLKKDYSFDLYHLAKKHQAMGQ
                     FEMSLEHIKNELGLPKSYDKLCNLKDRVMKPSLNEITTNTDIELTYENVKKGRSVVGFK
                     FIVKEKPKPKLIAPQRDQRTIDMFCNLSDAQINKYSTILSKLSELSDLSNFPDYPTFAL
                     WISGILRDPKSVREETTKRIFKALHSKTDFKP"
                     /name="replication initiation protein"
     gene            complement(3407..5059)
                     /gene="mobQ"
                     /locus_tag="QBJ73_RS19825"
                     /old_locus_tag="QBJ73_19830"
     CDS             complement(3407..5059)
                     /gene="mobQ"
                     /locus_tag="QBJ73_RS19825"
                     /old_locus_tag="QBJ73_19830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005199240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_004870770.1"
                     /translation="MAIYHFSVKTISRGNGRSAVACAAYRSSEKLVCDFYGKEQDYTRK
                     TGVEFTKIYAPENTNTELLKRQTLWNEVEKVERRKDALLAREFEIAFPSELNAEQRENM
                     LNELCRNLVKKYGVIVDAAIHAPHTDSGSDERNYHAHIMFTTRSINEQGGFSAKKYRDF
                     SRDNGTETVSHWRESFAELCNHHLEQNGFDERVDHRSYEDQESDLEATQHEGPQATYLR
                     RRGIFTEISLKNDEIKLRNLKIKKVIALDENIKVSEDLVQKVRSELQFQYSQAEYSEKT
                     SQKLYEFQNEELSKLTTKLDTMSSAISELRKKEPLFFKTKWINQINDLIDQHNTQLDIQ
                     KHISGLSEIEKKERYSDHLNKWTEENQLLQPKKSFAKFDEPNITVKQRKLNDQISNIDH
                     QISEISRRETEYQEYVRDQRLEIINSYIFEEDNYGNKYFSPQNGEKQKRLYAEEMEDLK
                     IQELHAAFTKKIETEAQQEFSQKRAIQLENDRKDRESREQQLRKEREQQEQRYAQEREQ
                     QEHLAFVRRQEQEKQQRNEPKKPENDNNHDYTP"
                     /name="mobQ"
     gene            5271..5573
                     /locus_tag="QBJ73_RS19830"
                     /old_locus_tag="QBJ73_19835"
     CDS             5271..5573
                     /locus_tag="QBJ73_RS19830"
                     /old_locus_tag="QBJ73_19835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000110201.1"
                     /translation="MSVETLEQKIAKQEERLRQLKAQKQAIAAREKKKNSDRQRKDDTR
                     RKILLGAWVLNKLKNDESFKGQLTDFEKFLSTESKTEENRQKDKDLFNGVIWNNT"
                     /name="hypothetical protein"
     gene            complement(5635..5898)
                     /locus_tag="QBJ73_RS19835"
                     /old_locus_tag="QBJ73_19840"
     CDS             complement(5635..5898)
                     /locus_tag="QBJ73_RS19835"
                     /old_locus_tag="QBJ73_19840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004674113.1"
                     /GO_process="GO:0008219 - cell death [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="WP_000184943.1"
                     /translation="MTNRNVEWTDNAWDEYIYWQTQDKKILKRINTLIKECQRTPFEGT
                     GKPEPLKANLSGFWSRRIDEKHRLVYEVTDERISIIQCRFHY"
                     /name="Txe/YoeB family addiction module toxin"
     gene            complement(5885..6148)
                     /locus_tag="QBJ73_RS19840"
                     /old_locus_tag="QBJ73_19845"
     CDS             complement(5885..6148)
                     /locus_tag="QBJ73_RS19840"
                     /old_locus_tag="QBJ73_19845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016658858.1"
                     /GO_process="GO:0008219 - cell death [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system prevent-host-death
                     family antitoxin"
                     /protein_id="WP_000557944.1"
                     /translation="MHVFTYTDARNNLKSVLDKVIDDVDVAFITRKEGGHAVVMGQDHY
                     DSLMETLYLLSSPNNASRLNESIAQLRAGKTKHRELIEDDES"
                     /name="type II toxin-antitoxin system prevent-host-death
                     family antitoxin"
     Typing          complement(3618..5059)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agctgttggg ctagatgatt tttatataag aagttattta acaaaaatta atacatatgt
       61 tatttagtat atgagattat atgtgccatc ttttttaaag aaatttaacc taatgttttt
      121 taatcaaaaa tatttttttc aagcaagctt cattacgatg gttatcggtt ttggtatgaa
      181 tttaaatgca agtgaagtca gtcaaaatga gaaaaatatt gaaaaagttg ttacccaatc
      241 atttaagcca cttatagatg agtatggtgt agctggtatg gctataggcg taatttacaa
      301 cggtaaaagt tatgaaaaat attatggtgt ccgatctaaa gatacaaatg aaagcgtaaa
      361 tagtcagaca ctttttgagt taggttcttt aagtaaaact tttactgcta tttcaggaac
      421 atatgccaat aatcaaggta aaatatcgtt caacgatcat cctagtaaat acgtccctgc
      481 tttaaaaaat tcagaaattg ataaggtaaa tttattagag ttattaactt atacaagtgg
      541 caatttgcca ttacaatttc cagataatat caaaactgat aagcaaatat tagagtattt
      601 caaaaattgg aaagtaaaga atcctcctgg tacttatcgt gaatactcaa atccaagtat
      661 aggacttttt gggtatctaa ctgcaaaatc gatgaatgtt cctttctctt cattattaga
      721 gaagactgtt tttcctcaac ttaatttgaa acatacatac gtcaatgttc cagaagcaca
      781 aaaaacgaac tatgcttttg gttatgatga aaataataag ccaattcgag ttaatcctgg
      841 tccattgtcg gatgaagcat atggcgttaa atcaacactc ccagatatgc ttaagtttgt
      901 aaattcgaat cttaatgtag atacaaatag ccctgctatg aaaaaagcta tactggatac
      961 acacaaagga tattttaaag tttctgatag cggtatgaca caagcacttg gatgggaaat
     1021 gttttcttat cccactactt ctgaaattct acaggctagt aattcaaaac aaatcttatt
     1081 gggctcaaat cctgttgtaa aagaattatc gcaaccaaaa tctaaagttt ttcataaaac
     1141 aggttcgact aatggctttg gggcgtatgt tttatttatt ccagaagaag gatttggatt
     1201 ggttatgtta atgaataaaa agataccaaa tgtagatcgt attaaggctt catataatgt
     1261 ttttgagaca ttgaaagata attaactttc tctaaaatta acataataca ccttatacga
     1321 aatgcgttat aattatcctt ataattcaac gctgtagttt caattttcta gcccagtcgt
     1381 caatgactgg gcttttttta ttacttttca cgttccacgc ctagtcttta atcaatgttc
     1441 cacgattttg attaaataaa ctgaataatt caatctgcag aatttaagcg attatcttga
     1501 ataagatcac tcaacttacc ttcaagcgat tcgattttat atttcagaga caagattgaa
     1561 tcttttaaac gtgaattttt aggtgcatcc ggattcttta attgattaaa aacctctaat
     1621 aattcgctgt gatgagtgct taacctttca atgtcacttt caatagccat tcttggtgtc
     1681 cacagagcaa gtaaactttg ctcataatct ttgagttctt gatgactcat tgcatctaat
     1741 tcagccttag ttctcatggt ttaaaatccg tcttgctatg aagagcttta aaaatccgtt
     1801 ttgttgtttc ctctcttaca cttttcggat ctcgtaagat gccactaatc cagagggcaa
     1861 atgtcgggta gtctgggaaa ttactgaggt ctgatagctc agaaagttta gataaaatag
     1921 tgctgtactt gttaatttga gcatcagata aattgcagaa catatctata gttctttgat
     1981 ctcgctgagg cgctattaat tttggcttag gtttttcttt cactataaat ttaaagccaa
     2041 ctactgaacg cccttttttt acattttcat aagtgagttc aatatctgta ttagttgtaa
     2101 tctcatttaa tgaaggcttc attactcggt cttttaaatt acagagtttg tcataagatt
     2161 tgggcaaacc cagctcattt tttatatgtt ctaaggacat ttcaaattgt cccattgctt
     2221 gatgcttttt agctaaatga taaagatcga atgagtaatc ttttttcaaa cttagtacga
     2281 tatctttttt atattttgta taagggttta atttatcaaa ctcttttaac atcagtagaa
     2341 cgatttcagg gaaacgtata gctatccctc cgtcttcata agtgcaatca atcacccaat
     2401 tcgacagcac cttttttcct ctttcattgt tataagagaa atttctttta agtagattct
     2461 tgctggctag ttctaactgc gtatatgcgg aatgcgtttt aatgccacat tctctagcaa
     2521 actcttctgc cgtaatcata attcggtctt tttcagttgc atcagtggtt ctagcaattg
     2581 ggctagcaag tattaacatt cgtttttcat caatgttcat actcttaaag cattcttgaa
     2641 cattgttttg cataacaacc cagctcagag gatatgtttt tttattttcg tccatataac
     2701 tacaacccca aaaaactaac taactacatt ggtatttatg tagcttaaac atacattgtc
     2761 gtttattttt tgactactat ttttgtagtt ttttaacaaa actacaaact ttttcttgta
     2821 gtttaatgtc cggtaaaagt agtttctatg tccggtaaaa gtagtttttt aagtccattt
     2881 tgtccggcat ttgtagttta atgtccggta aaagtagttt tttaaagtct gaaagcattg
     2941 ctaggcgtgg tttatagggg ggtctaaaag cttttaaaag ctttttaaaa gaataaaagg
     3001 gaatttattt ccaaagtgag ttattaaaac tgcccttggt actcgctaaa gctcgtactc
     3061 tattgaagct cgtttcactc gcttcgtggg gggcagtttt ttgcatatat gatcaaggat
     3121 tcaaaaagca aaacatgaat aatgcattcg ctatgctcat gctttttgac gagcaaaagc
     3181 atcaatactt cgctcgtaga cactcgctaa tcactgtccc cagtaattca ataaaaaacg
     3241 gtttttcaga gggggaataa gcaaactttc gttgtgtcgc tcgtagatac tcgctaaaag
     3301 gctctcattt gcgttctaag cgattttatt tttatcaact atagttttac tgtatttcta
     3361 tcaaaagtcg ctaaaacccc cgtttttttg cgcttaaatg ggatttctaa ggcgtgtagt
     3421 catggttatt gtcattttct ggctttttag gctcatttct ctgctgtttt tcttgttctt
     3481 ggcgtcttac aaatgccaaa tgttcttgtt gctctcgttc ttgtgcatag cgttgttctt
     3541 gttgctctcg ttctttacgt aactgctgtt ctctagattc acgatctttt cggtcgtttt
     3601 cgagctgtat tgctcttttt tgggaaaact cttgctgcgc ttcggtctca attttttttg
     3661 taaaagccgc atgcaattct tggattttta gatcttccat ttcttctgca tatagtcgct
     3721 tttgtttttc accattttga ggactaaagt atttatttcc atagttatct tcttcaaata
     3781 tataactatt tatgatctcc agtctttgat cgcgtacata ctcttgatac tcagtttctc
     3841 tgcgtgatat ttcagaaatt tgatgatcta tatttgaaat ttgatcatta agttttcgtt
     3901 gtttaacagt gatgttcggt tcgtcaaatt ttgcaaaaga ctttttaggc tggagcagct
     3961 gattttcttc tgtccattta tttaaatgat cagaatatct ctcttttttt tctatttcac
     4021 taagaccaga aatatgcttt tggatgtcta gttgggtgtt gtgttggtcg atcaggtcat
     4081 ttatttgatt gatccatttt gttttaaaaa ataaaggctc ttttttgcgt agttctgata
     4141 ttgcagaact catcgtgtct aattttgttg ttaattttga taattcttca ttttggaatt
     4201 cataaagttt ctgagaagtt ttttctgaat actcagcttg agagtattga aactgaagct
     4261 cacttcgtac tttttgaaca agatcctcag atactttgat gttttcatcc agtgcgatga
     4321 cctttttgat ttttaaatta cgtagcttaa tttcatcatt tttcagacta atttctgtaa
     4381 atatccctcg tctccggagg tatgtcgctt gaggaccttc gtgctgagtt gcttctaggt
     4441 cactttcctg gtcttcataa cttctgtgat cgacacgttc atcaaatcca ttttgttcca
     4501 ggtgatgatt gcataactct gcaaatgatt ctctccagtg gcttacagtt tctgtgccat
     4561 tgtcacgact gaagtcccta tatttttttg ctgaaaagcc accctgctca ttaatacttc
     4621 gggttgtgaa catgatgtgt gcatggtagt tgcgttcatc acttccactg tctgtatgag
     4681 gtgcatgaat agcagcatca acgatgacac cgtatttttt aacgaggttt cggcaaagtt
     4741 cattgagcat attttcacgc tgttcagcgt ttaactcact gggaaatgca atctcaaact
     4801 ctcttgcaag taatgcatct tttctacgtt caaccttttc gacttcattc caaagtgttt
     4861 ggcgcttgag taactcagta tttgtatttt caggtgcata gatttttgta aattcgacac
     4921 cagtttttct tgtgtagtct tgttcttttc cataaaaatc gcataccaat ttttcacttg
     4981 aacgatatgc agcacaagca acggccgatc gtccattacc tctactaatc gttttaactg
     5041 aaaaatgata tatcgccatt ttgcttttgc tctttatttc gatttatcga aattaccaac
     5101 atgtatatgt tggtcagggg attacaaagg ggatttaccc cttgtcgcac cattggatga
     5161 aatccaatgg ctaagtgcgc ccttcgggaa aaccacgata gcatgggctg tcttgaattt
     5221 caattcaaac agacctatgc ttgaggggga ttccttgaga tggtgatggt atgagtgttg
     5281 aaactttaga gcaaaaaatt gcaaagcaag aagaacgatt aagacaacta aaagcacaaa
     5341 aacaagctat tgctgctcgt gaaaaaaaga aaaattctga tcgacaaaga aaagatgata
     5401 ctagacgtaa aattctactg ggagcttggg ttctaaacaa actgaaaaat gatgaatctt
     5461 ttaagggtca actaaccgat tttgaaaagt ttttaagcac ggaaagtaag actgaagaaa
     5521 ataggcagaa agataaagat ctttttaatg gcgttatatg gaataacacc taaatttcgc
     5581 atatgagatt ttgtattaaa taaaaaaaca atcccctaaa aataggggat cttgttagta
     5641 atggaatcga cattgaatta tagagattcg ttcatctgtc acttcataaa ctaatctatg
     5701 cttttcatca atcctacgac tccaaaatcc tgaaagatta gcttttaaag gttctggttt
     5761 tcctgttcct tcaaaaggtg ttcgctgaca ttctttgatt aaggtattaa tacgcttaag
     5821 tatcttttta tcctgtgtct gccaatagat atattcatcc caggcattat cagtccattc
     5881 gacgttacga ttcgtcatcc tcaatcaact ctctgtgttt tgttttccct gcacgtaatt
     5941 gagcaattga ttcatttaaa cgacttgcat tatttggaga tgaaaggaga tataacgttt
     6001 ccattaagct atcataatga tcttgtccca ttactacagc atggccacct tcttttcttg
     6061 taataaaagc aacgtctaca tcatcaatta ctttatctaa aacagatttt aaattgttac
     6121 gtgcgtccgt atatgtaaat acatgcatat atctatccct catagggtaa gctgcgtcaa
     6181 aacgctgcta gctagactac ctaggcggta gtgattgaaa ctttacaatc ctaaatgtac
     6241 agaatactgt acatattgtc aatttcgtat aacagccatt atgttaaat
//
