LOCUS       NZ_CP121782             8636 bp    DNA     circular CON 05-SEP-2023
DEFINITION  Acinetobacter johnsonii strain AYTCM plasmid pAYTCM-6, complete
            sequence.
ACCESSION   NZ_CP121782
VERSION     NZ_CP121782.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN34117139
            Assembly: GCF_030867405.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter johnsonii
  ORGANISM  Acinetobacter johnsonii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 8636)
  AUTHORS   Tian,C., Wang,S., Fan,X. and Xia,D.
  TITLE     Acinetobacter johnsonii isolate AYTCM encoding NDM-1, OXA-58 and
            PER-1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8636)
  AUTHORS   Tian,C., Wang,S., Fan,X. and Xia,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) Department of Pharmacy, Department of
            Pharmacy, Shaoxing Hospital of Traditional Chinese Medicine
            Affiliated to Zhejiang Chi, Renminzhong Road 641, Yuecheng district,
            Shaoxing, Zhejiang 312000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/25/2023 18:02:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,979
            CDSs (total)                      :: 3,866
            Genes (coding)                    :: 3,724
            CDSs (with protein)               :: 3,724
            Genes (RNA)                       :: 113
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 57 of 142
            Pseudo Genes (incomplete)         :: 88 of 142
            Pseudo Genes (internal stop)      :: 30 of 142
            Pseudo Genes (multiple problems)  :: 29 of 142
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP121782.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8636
                     /organism="Acinetobacter johnsonii"
                     /mol_type="genomic DNA"
                     /strain="AYTCM"
                     /isolation_source="patient"
                     /db_xref="taxon:40214"
                     /plasmid="pAYTCM-6"
                     /geo_loc_name="China: Zhejiang"
                     /collection_date="2018-03-19"
     gene            complement(join(8631..8636,1..474))
                     /locus_tag="QBJ73_RS19710"
                     /old_locus_tag="QBJ73_19715"
     CDS             complement(join(8631..8636,1..474))
                     /locus_tag="QBJ73_RS19710"
                     /old_locus_tag="QBJ73_19715"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="WP_065995571.1"
                     /translation="MKPVLLIIDVQSTFNPPNWLIQKLEKLLPSVHSIATIELHDESIV
                     PFKSQLNWAPSEFDECPLKVEKVFIKYGYSPTDDLIQYLKGLDTQQVYVCGIQTDTCVL
                     AAGFRLFDAGLFPCLVTDLTVGSSLDRSGNLGIQLWKHHFRSITTSENLSSNTMI"
                     /name="cysteine hydrolase family protein"
     gene            complement(1462..2103)
                     /locus_tag="QBJ73_RS19715"
                     /old_locus_tag="QBJ73_19720"
     CDS             complement(1462..2103)
                     /locus_tag="QBJ73_RS19715"
                     /old_locus_tag="QBJ73_19720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016927662.1"
                     /GO_process="GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="WP_004787586.1"
                     /translation="MSKKDIAPQRLTREIVLRTALDMLNEEGIDSITTRKLAQRLGIKS
                     PTLYWHFKNKSLLMEAMAETIINEHHLVSLPIDGMTWQDWLLANSVSFRRALLAYRDGA
                     RLHARTSPSQGHFNTIEAQVALLSHAGFSPVEAVALLMTLGRFIVGWVLEEQQEEIRSD
                     PPFEADPTIYPLMLQGVNTLQNMNADDIFENGIRMVIIGAERQLDIKMQT"
                     /name="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
     gene            2169..3356
                     /locus_tag="QBJ73_RS19720"
                     /old_locus_tag="QBJ73_19725"
     CDS             2169..3356
                     /locus_tag="QBJ73_RS19720"
                     /old_locus_tag="QBJ73_19725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004856455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(39)"
                     /protein_id="WP_004856455.1"
                     /translation="MKKSLSVILITIFLDAVGIGLIMPILPELLRSLAGAEAGGVHYGA
                     LLAVYALMQFIFAPILGALSDRFGRRPVLIISIAGATADYLLMAAAPSLLWLYIGRIFA
                     GITGANMAVATAYVSDITPAHERAKRFGLLGAVFGIGFIAGPVIGGVLGEWNLHAPFFA
                     AAFMNGINLIMTAVLLKESKHSNKMTEKVQEQSILKKLSYLITQPNMAPLLGIFLIITL
                     VSQVPATLWVIYGQDRYGWSIFIAGVSLASYGICHSIAQAFAIAPMVKRFGEKNTLLCG
                     IACDAIGLLLLSIAVEEWVPFALLPLFALGGVAVPALQAMMSRGISDERQGELQGLLSS
                     FNSLGAIIGPVLVTSLYFMTQASAPGMVWALAAILYVITLPLLLKYRLNKYSGVP"
                     /name="tetracycline efflux MFS transporter Tet(39)"
     gene            complement(3586..4104)
                     /locus_tag="QBJ73_RS19725"
                     /old_locus_tag="QBJ73_19730"
     CDS             complement(3586..4104)
                     /locus_tag="QBJ73_RS19725"
                     /old_locus_tag="QBJ73_19730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017397202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_087090660.1"
                     /translation="MKSLSVLELSKLYNINRQTIYNQINKGILSKNSDNKIDFSEAIRV
                     FGEPARKQDVKEPVKIDSPTSAEVLLLRQQIDMLKNQVSDAKDREAFYQEQIEVMQRLL
                     EAPKPHVTTPVDPEPTQSKPETEEKEEVITIPPKEPEVIENKRILVPEHVQPEPEKRGF
                     WSRFFRPYD"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(4183..5094)
                     /gene="repM"
                     /locus_tag="QBJ73_RS19730"
                     /old_locus_tag="QBJ73_19735"
     CDS             complement(4183..5094)
                     /gene="repM"
                     /locus_tag="QBJ73_RS19730"
                     /old_locus_tag="QBJ73_19735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004650138.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_308469724.1"
                     /translation="MSKNIVVKANILIEASYKLDLVEMRLILLAIIEARESKTLIDANS
                     LIKISAKKYAEMFDISLKNSYSMLRGACKTLFDRQFSYVDWDFDLLSEDTSKIFFTSRW
                     VSKVGYKPGFVYLSFAPDVIPLISRLEKEFTRYDLLQISKFSSIYAVRIYEICMQWKIA
                     KKFYISLSDLRNTLGLTEIELSRIDNFQKKVLEVAKDQINESSDITIDYKTKKSGTKIT
                     GFEFTIRSKTSQPIKMEIALNDKQILLFSNKLVHEPSFASKYSKVGESYDDFAIRISEN
                     LTIPDKVQEYLPYLEKVGFNIA"
                     /name="repM"
     gene            complement(5745..7406)
                     /gene="mobQ"
                     /locus_tag="QBJ73_RS19735"
                     /old_locus_tag="QBJ73_19740"
     CDS             complement(5745..7406)
                     /gene="mobQ"
                     /locus_tag="QBJ73_RS19735"
                     /old_locus_tag="QBJ73_19740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005199240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_308469723.1"
                     /translation="MAIYHFSVKTISRGNGRSAVACAAYRSGEKLVCNFYGKEQDYTKK
                     TGVEFTEIYAPENTNTELLNRQTLWNKVEKAERRKDALLAREFEIAFPSELNAEQRENM
                     LNELCRNLVKKYGVIVDAAIHAPHTDSGSDERNYHAHIMFTTRSINEQGGFSAKKYRDF
                     SRDNGTETVSHWRESFAELCNHHLEQNGFDERVDHRSYEDQESDLEATQHEGPQATYLR
                     RRGIFTEISLKNDEIKLRNLKIKKVIALDENIKVSEDLVQKVRSELQFQYSQAEYSEKT
                     SQKLYEFQNEELSKLTTKLDTMSSAISELRKKEPLFFKTKWINQINDLIDQHNTQLDIQ
                     KHISGLSEIEKKERYSDHLNKWTEENQLLQPKKSFAKLDEPNITVKQRKLNDQISNIDH
                     QISEISRRETEYQEYVRDQRLEIINSYIFEEDNYGNKYFSPQNGEKQKRLYAEEMEDLK
                     IQELHAAFTKKIETEAQQEFAKKRAIQLENDRKDHELREQQLRKEREQQEQLYVYEQER
                     REQEHMAFLRRQELEKQQKNEPKKPEQDNDNDYTPW"
                     /name="mobQ"
     gene            7618..7920
                     /locus_tag="QBJ73_RS19740"
                     /old_locus_tag="QBJ73_19745"
     CDS             7618..7920
                     /locus_tag="QBJ73_RS19740"
                     /old_locus_tag="QBJ73_19745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000110201.1"
                     /translation="MSVETLEQKIAKQEERLRQLKAQKQAIAAREKKKNSDRQRKDDTR
                     RKILLGAWVLNKLKNDESFKGQLTDFEKFLSTESKTEENRQKDKDLFNGVIWNNT"
                     /name="hypothetical protein"
     gene            complement(7982..8245)
                     /locus_tag="QBJ73_RS19745"
                     /old_locus_tag="QBJ73_19750"
     CDS             complement(7982..8245)
                     /locus_tag="QBJ73_RS19745"
                     /old_locus_tag="QBJ73_19750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004674113.1"
                     /GO_process="GO:0008219 - cell death [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="WP_000184943.1"
                     /translation="MTNRNVEWTDNAWDEYIYWQTQDKKILKRINTLIKECQRTPFEGT
                     GKPEPLKANLSGFWSRRIDEKHRLVYEVTDERISIIQCRFHY"
                     /name="Txe/YoeB family addiction module toxin"
     gene            complement(8232..8495)
                     /locus_tag="QBJ73_RS19750"
                     /old_locus_tag="QBJ73_19755"
     CDS             complement(8232..8495)
                     /locus_tag="QBJ73_RS19750"
                     /old_locus_tag="QBJ73_19755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016658858.1"
                     /GO_process="GO:0008219 - cell death [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system prevent-host-death
                     family antitoxin"
                     /protein_id="WP_000557944.1"
                     /translation="MHVFTYTDARNNLKSVLDKVIDDVDVAFITRKEGGHAVVMGQDHY
                     DSLMETLYLLSSPNNASRLNESIAQLRAGKTKHRELIEDDES"
                     /name="type II toxin-antitoxin system prevent-host-death
                     family antitoxin"
     ARG             2170..3353
                     /name="tet(39)"
                     /gene="tet(39)"
                     /gene_name="tetracycline efflux MFS transporter Tet(39)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             2236..3356
                     /name="tet(39)"
                     /gene="tet(39)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="100.0"
                     /coverage="94.43"
     Typing          complement(5752..7406)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.928"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 catcgtatta gaagataaat tttcagatgt agtaattgat ctaaaatgat gtttccaaag
       61 ttgaattcct aaatttccac ttcgatctaa agaagaacct acagttaaat ctgtaactaa
      121 gcaggggaaa agaccagcat caaataatct aaaaccagca gcaagaacac acgtatccgt
      181 ttggatacca catacataaa cttgttgagt atctaaacct ttcaaatact gaatcaagtc
      241 atctgttgga gaatatccat acttaataaa tactttctct acttttagtg gacattcatc
      301 aaattccgat ggagcccaat ttagctggct tttgaaggga actattgatt catcatgtag
      361 ttctatagtc gcgattgaat gaaccgaagg cagcaatttt tctaattttt gtattaacca
      421 gttaggagga ttaaaagttg attgaacatc aattattaac aggactggtt tcataaataa
      481 taacaaatta gttttagtaa attgatttta tcaaagagaa gccttttaag agctactaaa
      541 attaatataa tatgccttat aagaaatctg aaaaaaacaa tttaaatcaa tattttaatg
      601 tgtttttaaa aaattcacat aacagccatt atgtaaaaag gcttaatcaa aataacctaa
      661 aattgctaaa gtaggtataa aaaagatcgc aaatacacca aaatattgaa tcatcttctt
      721 atgtctgaaa tctcgtttag aaatgatcaa aagcaaagct gaaaaagaaa tgactattaa
      781 tgtccatgaa attgaataca tacttaaatc ctaattaatt atgttgggct ttaaatttct
      841 ctaaaattaa cataatacac cttatacgaa aggcccataa attgggcttt ttggtcatgc
      901 atttaacata aaatctcatc tacgaaatcc agattcaaga cataaaaaag cccgacatcc
      961 tgtcgggctt ttttagattg ggttgctcgt cttgctgatc tcgtgctgcc cattccttgc
     1021 gctactttct tattcttgtt tttttggaaa catcctgttc cctgtatagc catcatagga
     1081 ttaatgcctt gaattaccaa tccgcaaaat ccttaaatct atgtaagttt tagcttacaa
     1141 agttggccta tttttatccc aagagttgtc cgcagaaatt ggggatgatt taagctctct
     1201 atggattgat aatgcacact aaagtttcat tttgattaaa aaatcatcaa aaacattggc
     1261 aagaagtatc aaatccgttc ataaaaaagc cgacttgttt ccaagtcggc tttttaggtt
     1321 ttgcgtccca attttttata gcataacttt ttgaaatata aaactattta tgagtacgtt
     1381 tcgtataagc tctattatgt taaataatta aggataaaag ctttattact aaatagaaca
     1441 gttgcttaaa gcagtataga gttaagtttg cattttaata tcaagttgtc tttcagcacc
     1501 gataatcacc atacgaatcc cattttcaaa aatgtcatcc gcattcatat tttgtaaggt
     1561 attcactcct tggagcatga gtggatatat agttggatcg gcttcgaatg gtgggtctga
     1621 tcttatttct tcttgctgct cctcaaggac ccacccaaca ataaaccttc ctaatgtcat
     1681 aagtaatgca actgcttcaa caggagaaaa cccagcatga gataacagcg caacttgggc
     1741 ctcaatcgtg ttgaaatgtc cttgactggg gctagttctt gcatgtagac gagcaccatc
     1801 acggtaagct agtagtgctc gacgaaagct tacagaatta gcgagcaacc aatcttgcca
     1861 tgtcatccca tcgatgggta aagaaactaa atggtgttca tttataatgg tttccgccat
     1921 tgcttccatt aacaaggatt tattcttgaa atgccaatat agagttggag atttaatacc
     1981 caatcgttga gctagcttgc gtgtggttat tgaatctatg ccttcttcat tcaacatatc
     2041 caatgcggta cgcaggacta tttcacgtgt cagacgttga ggtgcaatat cttttttact
     2101 cataaatcta tcactgatag ggagtgtgtg agagaatatt aattctatca ttgctagaga
     2161 aggcatttgt gaagaaatca ttgagcgtga ttttaatcac tatatttctg gatgctgttg
     2221 ggattggttt aattatgccg atcttgcctg aattattacg gtcattggct ggagctgaag
     2281 caggcggtgt tcactatggt gctttattag ctgtgtatgc tctgatgcag ttcatttttg
     2341 cacctatcct tggagcgttg agtgaccgat ttggacgtcg acctgtatta attatttcaa
     2401 ttgctggtgc aacggctgat tatctcctaa tggctgctgc tccttctcta ttgtggctat
     2461 atattggtcg tatttttgcg ggaattacag gtgccaacat ggctgttgca acagcttatg
     2521 tttcagatat tactccagcc catgagcgtg caaaaaggtt tggtctcctt ggagctgtct
     2581 ttggtattgg gtttatagcg ggtccggtaa taggtggagt tttgggtgaa tggaacttac
     2641 atgcaccgtt ctttgctgct gcttttatga atgggattaa tttaataatg acagcagtct
     2701 tattaaaaga atcaaaacac agcaataaaa tgactgagaa ggttcaggag caatcaatat
     2761 taaagaaatt atcctatttg atcactcaac ctaatatggc tccattgctt ggtatctttt
     2821 taattatcac attggtttca caagtccccg caactttatg ggttatctat gggcaggatc
     2881 gttatggctg gagtatattt attgcaggtg tttcccttgc tagttatgga atatgccatt
     2941 ctattgcaca ggcttttgct atcgccccta tggtaaagag gtttggagag aaaaatacgt
     3001 tgttatgtgg aatagcttgc gatgcaattg gtttacttct tttatctatt gctgttgaag
     3061 aatgggtgcc ttttgcgttg ttaccattgt ttgcccttgg tggagtagcc gttcctgctt
     3121 tgcaagcaat gatgtccaga ggtattagtg atgaaagaca aggtgaatta caagggctat
     3181 taagcagttt taatagtctg ggggctataa ttggtcctgt attagttact agcctctatt
     3241 ttatgactca ggcatcagct cctggaatgg tatgggcatt agctgcaata ctttatgtaa
     3301 tcaccctacc cttattgctt aagtatcgcc tgaataaata ttctggagtt ccataaaata
     3361 tttaatttat tggtttaaaa actaatggat tctatagttt atctaaaatt aacataatac
     3421 acgttatacg aaacgtgtta taatttacct tgaaattcat atacttattt caaagtttat
     3481 agcccgagca tcactgttcg ggcttttttt atgatttttc acgttccacg cttattattt
     3541 gatcaatgtt tcacgacttt gttcatatca gttaaacaat cgtagttaat catagggtct
     3601 aaaaaaacgg ctccaaaagc ctcttttttc cggttccggt tggacatgtt ccgggactag
     3661 aatgcgctta ttctcgatta cttcaggttc ttttggaggg attgtgatca cttcctcttt
     3721 ttcttctgtc tcaggcttag attgagttgg ctcaggatca acaggtgtag tgacatgagg
     3781 ttttggagct tccaatagac gttgcatgac ttcaatttgt tcttggtaga aggcttctcg
     3841 atctttggca tctgatactt gatttttcag catgtctatc tgctgtctaa gcagtaaaac
     3901 ttctgccgaa gttggactgt ctatttttac aggctctttt acatcctgtt ttcttgcagg
     3961 ttcaccaaat acacggatgg cttctgagaa atcaatctta ttatcagaat ttttacttaa
     4021 aattcctttg tttatctgat tatagatggt ctgtctattg atattgtaaa gcttggataa
     4081 ctctaaaact gaaaggcttt tcatgatgta aagtgatgcc taatcttgta aaaatgtctt
     4141 gtctattaga ctgtctaata gacaaaacga caagtctata ttttaagcaa tattgaaccc
     4201 gactttttct agatagggta agtattcctg aaccttatca ggaatagtta aattttcaga
     4261 aattcttata gcgaagtcat cataactttc accaactttg ctgtactttg aagcaaatga
     4321 aggttcatga actagcttat ttgagaacaa aagaatttgt ttatcattta aggcgatttc
     4381 cattttaatt ggctgtgatg ttttggatct tattgtgaac tcaaagccag ttatcttggt
     4441 tccagacttt ttggttttat aatcaatagt tatgtccgaa gactcattaa tttgatcttt
     4501 tgctacttca aggactttct tttgaaagtt atcaattctt gaaagttcta tttctgttaa
     4561 acctaatgta tttcgtaaat cagagagaga aatataaaac tttttagcaa tcttccattg
     4621 catacatatt tcataaatcc gaacagcata aatactagaa aacttactta tttgaagaag
     4681 gtcataacgc gtaaattctt tttctaagcg acttattaat ggtatgacat caggagcaaa
     4741 agataagtaa acaaaacctg gcttataacc taccttagat acccatcgag aagtaaaaaa
     4801 gattttagaa gtatcttcac ttaatagatc aaaatcccaa tccacatatg aaaattgtct
     4861 gtcgaacagg gttttacaag cccctcgtag catggaataa gagtttttga ggctaatatc
     4921 aaacatttct gcatactttt tagctgatat ttttatcaga gagttagcat caatcaatgt
     4981 tttactttct ctagcttcaa taattgctaa aagtattaag cgcatttcta ccaaatcaag
     5041 tttgtagctt gcctcaatca agatattcgc ttttacgact atatttttac tcattattag
     5101 accataaaat ttttattaat ttactatata tttttttttg taggaaataa agaagaaaac
     5161 gagataaaca tatactgcta aaggtaggga tatatactgc taaaggtagg gatatatact
     5221 gctaaaggta gggatatata ctgctaaagg tagggatata tactgctaaa ggtagggggt
     5281 gatcctctat aaccctttat ctacaagcct ttaaggtagg ctaaaaacat taaaaactat
     5341 taaaaacatt aaaaacatca attaagaaaa attgcccttg gttttcgcta cgctcaaact
     5401 ctattgaatc tcgctttcgc tcgattctag ggacaatttt ttggttaata catatatcaa
     5461 tttaaaaaaa tcaaaagcaa taatgcattc gctttgctca tgctttttga aaagcaaaaa
     5521 cttcattgtg acgctcgtag acacttgcta aagaaactct ctaataacac ctaaaaagcg
     5581 gtttttcaga ggggggaata agcgaacttt cattgtgacg ctcgtagaca ctcgctaaag
     5641 gctctcattt gaggtctaag agcttttact cttatagact atggtttccc tacattttta
     5701 gtaaaagtcg ctaaaaaccc cggttttttg cgtttaaaca gggtttacca aggtgtgtaa
     5761 tcgttatcat tgtcttgttc tggcttttta ggctcatttt tctgctgttt ctccaattct
     5821 tggcgtctta aaaatgccat atgttcttgt tctcgccttt cttgctcata gacatagagt
     5881 tgttcttgtt gttctcgttc tttacgcaac tgctgttccc tcaattcatg atcttttcgg
     5941 tcgttttcga gctgtatcgc tctttttttg gcaaattctt gctgcgcttc ggtctcaatt
     6001 ttttttgtaa aagccgcatg caattcttgg atttttagat cttccatttc ttctgcatat
     6061 agtcgctttt gtttttcacc attttgagga ctaaagtatt tatttccata gttatcttct
     6121 tcaaatatat aactatttat gatctccagt ctttgatcgc gtacatactc ttgatactca
     6181 gtttctctgc gtgatatttc agaaatttga tgatctatat ttgaaatttg atcattaagt
     6241 tttcgttgtt taacagtgat gttcggttcg tcaagttttg caaaagactt tttaggctgg
     6301 agcagctgat tttcttctgt ccatttattt aaatgatcag aatatctctc ttttttttct
     6361 atttcactaa gaccagaaat atgcttttgg atgtctagtt gggtgttgtg ttggtcgatc
     6421 aggtcattta tttgattgat ccattttgtt ttaaaaaata aaggctcttt tttgcgtagt
     6481 tctgatattg cagaactcat cgtgtctaat tttgttgtta attttgataa ttcttcattt
     6541 tggaattcat aaagtttctg agaagttttt tctgaatact cagcttgaga gtattgaaac
     6601 tgaagctcac ttcgtacttt ttgaacaaga tcctcagata ctttgatgtt ttcatccagt
     6661 gcgatgacct ttttgatttt taaattacgt agcttaattt catcattttt cagactaatt
     6721 tctgtaaata tccctcgtct ccggaggtat gtcgcttgag gaccttcgtg ctgagttgct
     6781 tctaggtcac tttcctggtc ttcataactt ctgtgatcga cacgttcatc aaatccattt
     6841 tgttccaggt gatgattgca taactctgca aatgattctc tccagtggct tacagtttct
     6901 gtgccattgt cacgactgaa gtccctatat ttttttgctg aaaagccacc ctgctcatta
     6961 atacttcggg ttgtgaacat gatgtgtgca tggtagttgc gttcatcact tccactgtct
     7021 gtatgaggtg catgaatagc agcatcaacg atgacaccgt attttttaac gaggtttcgg
     7081 caaagttcat tgagcatatt ttcacgctgt tcagcgttta actcactggg aaatgcaatc
     7141 tcaaactctc ttgcaagtaa tgcatctttt cttcgttcgg ctttttcgac tttgttccaa
     7201 agtgtctgac gattgagtaa ctcggtattt gtattttcgg gcgcatagat ttctgtaaat
     7261 tcgacaccag ttttttttgt gtagtcttgt tcttttccat agaaattgca taccaatttt
     7321 tcacctgaac gatatgcagc acaagcaacg gctgatcgtc cattacctct actaatcgtt
     7381 ttaactgaaa aatgatatat cgccattttg cttttgctct ttatttcgat ttatcgaaat
     7441 taccaacatg tatatgttgg tcaggggatt acaaagggga tttacccctt gtcgcaccat
     7501 tggatgaaat ccaatggcta agtgcgccct tcgggaaaac cacgatagca tgggctgtct
     7561 tgaatttcaa ttcaaacaga cctatgcttg agggggattc cttgagatgg tgatggtatg
     7621 agtgttgaaa ctttagagca aaaaattgca aagcaagaag aacgattaag acaactaaaa
     7681 gcacaaaaac aagctattgc tgctcgtgaa aaaaagaaaa attctgatcg acaaagaaaa
     7741 gatgatacta gacgtaaaat tctactggga gcttgggttc taaacaaact gaaaaatgat
     7801 gaatctttta agggtcaact aaccgatttt gaaaagtttt taagcacgga aagtaagact
     7861 gaagaaaata ggcagaaaga taaagatctt tttaatggcg ttatatggaa taacacctaa
     7921 atttcgcata tgagattttg tattaaataa aaaaacaatc ccctaaaaat aggggatctt
     7981 gttagtaatg gaatcgacat tgaattatag agattcgttc atctgtcact tcataaacta
     8041 atctatgctt ttcatcaatc ctacgactcc aaaatcctga aagattagct tttaaaggtt
     8101 ctggttttcc tgttccttca aaaggtgttc gctgacattc tttgattaag gtattaatac
     8161 gcttaagtat ctttttatcc tgtgtctgcc aatagatata ttcatcccag gcattatcag
     8221 tccattcgac gttacgattc gtcatcctca atcaactctc tgtgttttgt tttccctgca
     8281 cgtaattgag caattgattc atttaaacga cttgcattat ttggagatga aaggagatat
     8341 aacgtttcca ttaagctatc ataatgatct tgtcccatta ctacagcatg gccaccttct
     8401 tttcttgtaa taaaagcaac gtctacatca tcaattactt tatctaaaac agattttaaa
     8461 ttgttacgtg cgtccgtata tgtaaataca tgcatatatc tatccctcat agggtaagct
     8521 gcgtcaaaac gctgctagct agactaccta ggcggtagtg attgaaactt tacaatccta
     8581 aatgtacaga atactgtaca tattgtcaat ttcgtataac agccattatg ttaaat
//
