LOCUS       NZ_CP121631             2277 bp    DNA     circular CON 17-MAY-2024
DEFINITION  Acinetobacter baumannii strain JAB270 plasmid pJAB270-3, complete
            sequence.
ACCESSION   NZ_CP121631
VERSION     NZ_CP121631.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27010253
            Assembly: GCF_029714085.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Alghorbi,M.F., Bawazeer,R., Okdah,L., Alswaji,A., Alzayer,M.A.,
            Alrashidi,E., Alshahrani,A.S., Al Johani,S.M., NGHA Surveillance
            Group,N.S.G., Balkhy,H. and Doumith,M.
  TITLE     Antimicrobial resistance and genomic characterisation of
            Acinetobacter baumannii clinical isolates from a nation
            representative surveillance in Saudi Arabia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Alghorbi,M.F., Bawazeer,R., Okdah,L., Alswaji,A., Alzayer,M.A.,
            Alrashidi,E., Alshahrani,A.S., Al Johani,S.M., NGHA Surveillance
            Group,N.S.G., Balkhy,H. and Doumith,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-APR-2023) Infectious Diseases Research, King Abdullah
            International Medical Research Center (KAIMRC), KAIMRC, P.O Box
            22490, Riyadh 11426, Saudi Arabia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: EToKi v. 1.2
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029714085.1-RS_2024_05_17
            Annotation Date                   :: 05/17/2024 04:52:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,853
            CDSs (total)                      :: 3,758
            Genes (coding)                    :: 3,683
            CDSs (with protein)               :: 3,683
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 19 of 75
            Pseudo Genes (incomplete)         :: 47 of 75
            Pseudo Genes (internal stop)      :: 16 of 75
            Pseudo Genes (multiple problems)  :: 7 of 75
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP121631.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="JAB270"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pJAB270-3"
                     /geo_loc_name="Saudi Arabia:Jeddah"
                     /lat_lon="21.4858 N 39.1925 E"
                     /collection_date="2019"
                     /collected_by="KAIMRC AMR WGS_Based Surveillance Program"
     gene            complement(join(2060..2277,1..715))
                     /gene="repM"
                     /locus_tag="MTS25_RS19300"
                     /old_locus_tag="MTS25_19300"
     CDS             complement(join(2060..2277,1..715))
                     /gene="repM"
                     /locus_tag="MTS25_RS19300"
                     /old_locus_tag="MTS25_19300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005265321.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_000711927.1"
                     /translation="MKKELVVKDNALINASYNLDLSEQRLILLAILEARQSNTPNDKDL
                     TIHAESYINHFNVHRNTAYKVLKDACKSLFDRRFSYQKLTQKGNIENVISRWVQRISYV
                     ENEALVRIKFSDDVVPLITNLEKHFTSYELEQVSSLTSVYAIRLYELLIAWRSTGKVTM
                     VELEELRLKLGIEPNEYKRMGQFKEKVLHLAIDQINKYTDIKAEYEQHKRGRSIIGFSF
                     KFKQKQQPKKLDSKRDPNTPDFFIRMTDAQRHLFGNKLAHDARVQSEYSHLIGTGSYED
                     FAKLLADMLAEEQHFKMFYPLLVEHGYKA"
                     /name="repM"
     Typing          complement(2061..2277)
                     /name="rep_cluster_1280"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.1900000000000004e-109"
ORIGIN
        1 ggtcacgctt agaatctaat tttttcggtt gttgtttctg cttaaactta aatgaaaaac
       61 caataattga acgaccacgt ttgtgttgtt catactctgc ttttatatct gtgtacttat
      121 taatttgatc aatggcaagg tgtaaaactt tttctttaaa ttgccccatt ctcttgtatt
      181 cattgggttc tatgcctagt tttaatctta gctcttctag ctctaccata gtcactttgc
      241 ccgtactacg ccatgcgata agcaactcat acaagcgtat agcgtaaaca ctagttaaac
      301 tgctgacttg ctctagttca taactagtga agtgtttttc taaatttgta atcaacggta
      361 caacatcatc agaaaactta atacgaacaa gagcttcatt ctctacataa gatattcgtt
      421 gtacccatcg acttattaca ttctcaatgt tgcccttttg agtcagtttt tgatagctaa
      481 atctacgatc aaataggctt ttgcaagcat ctttaagaac tttataggct gtatttctat
      541 gaacattgaa atggttgata tagctttcag catgaatcgt caaatcttta tcattgggtg
      601 tatttgattg cctagcttca agtatggcta acagtattaa acgttgttct gataggtcta
      661 aattataact ggcatttatt agtgcattat ctttaacgac tagttctttt ttcataagca
      721 cacatgtctt ttaagtatat aagtacactc tattttatta agtgtcactt tgcaagataa
      781 aggtgtactt attgcaagat aaaggtgtac ttattgcaag ataaaggtgt acttattgca
      841 agataaaggt gtacttatcc ttatttttat tattatttat caattaatta cagaggctaa
      901 aaattaaaaa attaaaaatt attaaaaaga ggggagtgct taaaactgcc ctatgtactc
      961 gctaaagctc gtactctatt gaagctcgtt cctcgcttcg tggggcagtt tttagttaat
     1021 cctgtttgtg aaatctaaaa gcaaaagcat ttgtaatgta ttcgcttcgc tcatactttt
     1081 tgaaaagcaa aagctccaaa gttcgcaccc attcgggatg ctctgtacct accgtttaaa
     1141 atcgaaaggg agcttgtaat cagagaaaag gcgagggcga aattttgatg atgctcaatc
     1201 gctcgtagac actcgctcgg ttaattctat ctgtgctttc tatggtgctt ttattggatt
     1261 tttagtgcac cgataaaagc acagtaaaag gcacagacac ttcgcttcgc tgcgcctaaa
     1321 aacaattacc gtacaagctc gcttcgctcg tccttcctgg acgtcaggat tccttgtact
     1381 ttaagggctg gacgccctta ataatcgttt tttgttgcct ttcactttcc gctatcgctc
     1441 caagttgcag cgcacttcgt gctcgcgccc cgtcatcctc ctacggagtt gacccacccc
     1501 cttttaggtg catatttcgc ttaggctcgt atgtccttcg gattcgctga acgcttacac
     1561 taaaacgagg tgggtcaact ccccttcggg atagtttgac ggggtgggct tgccccccaa
     1621 caactaacga catctgggga tgtctaaagt tcgtgtccac tcccttttac gttgtcggct
     1681 ccccccagtc atctccgctg gggcttcgat gatgttccgc tttcgcttca caccccttcg
     1741 gggctttctt tggctaaagc caaaatgaaa agcacagata gatttttaga gcgagtgtct
     1801 acgagtgaac tgatgaaatg caaaaacaag cacaccctcc ctgattacat gctctatttg
     1861 ctcataggat gcgatatttc gagctttgct cgcagtgggt atgtgaatgt agcttgttgg
     1921 atttggatcg cttaaaacgc aatttagagg ggttggtggc tatccaccaa tattggcgta
     1981 ttggtggcgc caccttatct tgtacataga taaggaaggt cgaaatgact tatctaccat
     2041 tgatagaata cgacaagatt tatgctttgt aaccatgctc tacaagcaaa gggtaaaaca
     2101 tcttaaaatg ctgttcttct gcaagcatat ccgccagtaa ttttgcaaag tcttcatagc
     2161 ttccagtacc aattaaatgg ctgtattcgc tttgtacacg agcatcatgg gctagtttgt
     2221 tcccaaataa gtggcgttgt gcatcggtca ttctgataaa aaagtcaggt gtgtttg
//
