LOCUS       NZ_CP121611            21454 bp    DNA     circular CON 17-MAY-2024
DEFINITION  Acinetobacter baumannii strain JAB186 plasmid pIII_JAB186, complete
            sequence.
ACCESSION   NZ_CP121611
VERSION     NZ_CP121611.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27010238
            Assembly: GCF_029713865.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 21454)
  AUTHORS   Alghorbi,M.F., Bawazeer,R., Okdah,L., Alswaji,A., Alzayer,M.A.,
            Alrashidi,E., Alshahrani,A.S., Al Johani,S.M., NGHA Surveillance
            Group,N.S.G., Balkhy,H. and Doumith,M.
  TITLE     Antimicrobial resistance and genomic characterisation of
            Acinetobacter baumannii clinical isolates from a nation
            representative surveillance in Saudi Arabia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 21454)
  AUTHORS   Alghorbi,M.F., Bawazeer,R., Okdah,L., Alswaji,A., Alzayer,M.A.,
            Alrashidi,E., Alshahrani,A.S., Al Johani,S.M., NGHA Surveillance
            Group,N.S.G., Balkhy,H. and Doumith,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-APR-2023) Infectious Diseases Research, King Abdullah
            International Medical Research Center (KAIMRC), KAIMRC, P.O Box
            22490, Riyadh 11426, Saudi Arabia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: EToKi v. 1.2
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 179x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029713865.1-RS_2024_05_17
            Annotation Date                   :: 05/17/2024 05:27:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,031
            CDSs (total)                      :: 3,935
            Genes (coding)                    :: 3,817
            CDSs (with protein)               :: 3,817
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 47 of 118
            Pseudo Genes (incomplete)         :: 69 of 118
            Pseudo Genes (internal stop)      :: 17 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP121611.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..21454
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="JAB186"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pIII_JAB186"
                     /geo_loc_name="Saudi Arabia:Jeddah"
                     /lat_lon="21.4858 N 39.1925 E"
                     /collection_date="2019"
                     /collected_by="KAIMRC AMR WGS_Based Surveillance Program"
     gene            join(21446..21454,1..411)
                     /locus_tag="MTS34_RS20255"
     CDS             join(21446..21454,1..411)
                     /locus_tag="MTS34_RS20255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_346283696.1"
                     /translation="MQHKLIRNTELFFEHHWNVDLLGKPPEWSPVHFNFGKIPNYDKQG
                     VYAFVKGDLVTYIGVGTSRGAGRYRGNGLSARVMKYCRWNEDKTEYIAIDPRLKDAGSI
                     ITIGFERDTAYLANALEVFLISKIQPVHNVVKVGM"
                     /name="hypothetical protein"
     gene            complement(443..823)
                     /locus_tag="MTS34_RS20095"
                     /old_locus_tag="MTS34_20095"
     CDS             complement(443..823)
                     /locus_tag="MTS34_RS20095"
                     /old_locus_tag="MTS34_20095"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015060210.1"
                     /translation="MKYIAQIIECNENLEFILKIKVRNEILEVFSSSIPSCIDLKKNYL
                     IDLEATIFDDYLIEKTHYSHQIKQIDNSLRYEIIGYLKEGKLDVNGLIFEDDILNRDFF
                     YLDNNTVKWTVDRINADFDDRD"
                     /name="hypothetical protein"
     gene            1018..1629
                     /locus_tag="MTS34_RS20100"
                     /old_locus_tag="MTS34_20100"
     CDS             1018..1629
                     /locus_tag="MTS34_RS20100"
                     /old_locus_tag="MTS34_20100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004889724.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_015060246.1"
                     /translation="MIEMNTRIYLRASTKDQDAERALQILQDLNQNLNLGETIVYVENY
                     SGTKLDRPELNKLLSEANQGDTLLVESIDRLSRLTQRDFQELKRRIQEKGLRLVVADLP
                     TTYQMIQTSDSITHSILELINNMLIDLLATMARLDNEKRIERIKQGLARSGYKPTGKKA
                     NEAKHKRIKELLVVGNMTKEEIAKAVNCGVATVYRVAKVI"
                     /name="recombinase family protein"
     gene            complement(1819..2703)
                     /locus_tag="MTS34_RS20105"
                     /old_locus_tag="MTS34_20105"
     CDS             complement(1819..2703)
                     /locus_tag="MTS34_RS20105"
                     /old_locus_tag="MTS34_20105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(E) family macrolide 2'-phosphotransferase"
                     /protein_id="WP_000155092.1"
                     /translation="MTIQDIQSLAEAHGLLLTDKMNFNEMGIDFKVVFALDTKGQQWLL
                     RIPRRDGMREQIKKEKRILELVKKHLSVEVPDWRISSTELVAYPILKDNPVLNLDAETY
                     EIIWNMDKDSPKYITSLAKTLFEIHSIPEKEVRENDLKIMKPSDLRPEIANNLQLVKSE
                     IGISEQLETRYRKWLDNDVLWADFTQFIHGDLYAGHVLASKDGAVSGVIDWSTAHIDDP
                     AIDFAGHVTLFGEESLKTLIIEYEKLGGKVWNKLYEQTLERAAASPLMYGLFALETQNE
                     SLIVGAKAQLGVI"
                     /name="Mph(E) family macrolide 2'-phosphotransferase"
     gene            complement(2759..4234)
                     /gene="msr(E)"
                     /locus_tag="MTS34_RS20110"
                     /old_locus_tag="MTS34_20110"
     ARG             complement(2763..4234)
                     /name="msr(E)"
                     /gene="msr(E)"
                     /gene_name="ABC-F type ribosomal protection protein Msr(E)"
                     /tool="amrfinderplus"
                     /drug_class="MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(4727..4999)
                     /gene="nadS"
                     /locus_tag="MTS34_RS20115"
                     /old_locus_tag="MTS34_20115"
     CDS             complement(4727..4999)
                     /gene="nadS"
                     /locus_tag="MTS34_RS20115"
                     /old_locus_tag="MTS34_20115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_000369781.1"
                     /translation="MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTG
                     LSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLIRILDAHPSLI"
                     /name="nadS"
     gene            complement(4992..5312)
                     /locus_tag="MTS34_RS20120"
                     /old_locus_tag="MTS34_20120"
     CDS             complement(4992..5312)
                     /locus_tag="MTS34_RS20120"
                     /old_locus_tag="MTS34_20120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000897307.1"
                     /translation="MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGI
                     RKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAYPKSVKDNLTDKETAILHQLVKEQF
                     HG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            5488..6315
                     /locus_tag="MTS34_RS20125"
                     /old_locus_tag="MTS34_20125"
     CDS             5488..6315
                     /locus_tag="MTS34_RS20125"
                     /old_locus_tag="MTS34_20125"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057689930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
                     /protein_id="WP_000713530.1"
                     /translation="MKKFILPIFSISILVSLSACSSIKTKSEDNFHISSQQHEKAIKSY
                     FDEAQTQGVIIIKEGKNLSTYGNALARANKEYVPASTFKMLNALIGLENHKATTNEIFK
                     WDGKKRTYPMWEKDMTLGEAMALSAVPVYQELARRTGLELMQKEVKRVNFGNTNIGTQV
                     DNFWLVGPLKITPVQEVNFADDLAHNRLPFKLETQEEVKKMLLIKEVNGSKIYAKSGWG
                     MDVTPQVGWLTGWVEQANGKKIPFSLNLEMKEGMSGSIRNEITYKSLENLGII"
                     /name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
     gene            complement(6498..6641)
                     /locus_tag="MTS34_RS20130"
                     /old_locus_tag="MTS34_20130"
     CDS             complement(6498..6641)
                     /locus_tag="MTS34_RS20130"
                     /old_locus_tag="MTS34_20130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            complement(6661..7236)
                     /locus_tag="MTS34_RS20135"
                     /old_locus_tag="MTS34_20135"
     CDS             complement(6661..7236)
                     /locus_tag="MTS34_RS20135"
                     /old_locus_tag="MTS34_20135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_001096616.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTSQKIESSKQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATAAPHQDATACDTKMPQHATTQQDTDNKKNNELNIAENVAVPQQETTAYHTQTLQH
                     ATLQSVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(7229..8179)
                     /gene="repM"
                     /locus_tag="MTS34_RS20140"
                     /old_locus_tag="MTS34_20140"
     CDS             complement(7229..8179)
                     /gene="repM"
                     /locus_tag="MTS34_RS20140"
                     /old_locus_tag="MTS34_20140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001205343.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHADSYINQFGVHRNTAYQALKDACKDLFARQFSYQEKKANGNIRNVMSRWVSQIAY
                     NDNEATVDLIFAPAVVPFITRLEEQFTKYELQQVSSLSSAYAIRLYELLIQWRSTGKTP
                     TIELQEFRKKLGVLDNEYLRMAHLKERVLELSIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDSPSIERDPNTLELFSKMTDAQRHMFANKLSELPEMGRYSQGTESYQQFAV
                     RIAEMLQDPAQFKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            8863..9081
                     /locus_tag="MTS34_RS20260"
     CDS             8863..9081
                     /locus_tag="MTS34_RS20260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002031357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015062952.1"
                     /translation="MNGQKIDFLRFSKQGNKYFFPCKTKHRPYVTLANFPIQDPAHAGI
                     FLSHTTHLKMMTFKALFHKVFGCFYRF"
                     /name="hypothetical protein"
     gene            9315..9506
                     /locus_tag="MTS34_RS20145"
                     /old_locus_tag="MTS34_20145"
     CDS             9315..9506
                     /locus_tag="MTS34_RS20145"
                     /old_locus_tag="MTS34_20145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000032259.1"
                     /translation="MSKELVNLANIVPFPKSCGFCESKENVQLFAGLMLCSSCQENIRT
                     TNPGMFTANKQIEKKAQD"
                     /name="hypothetical protein"
     gene            complement(9550..10377)
                     /locus_tag="MTS34_RS20150"
                     /old_locus_tag="MTS34_20150"
     CDS             complement(9550..10377)
                     /locus_tag="MTS34_RS20150"
                     /old_locus_tag="MTS34_20150"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057689930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
                     /protein_id="WP_000713530.1"
                     /translation="MKKFILPIFSISILVSLSACSSIKTKSEDNFHISSQQHEKAIKSY
                     FDEAQTQGVIIIKEGKNLSTYGNALARANKEYVPASTFKMLNALIGLENHKATTNEIFK
                     WDGKKRTYPMWEKDMTLGEAMALSAVPVYQELARRTGLELMQKEVKRVNFGNTNIGTQV
                     DNFWLVGPLKITPVQEVNFADDLAHNRLPFKLETQEEVKKMLLIKEVNGSKIYAKSGWG
                     MDVTPQVGWLTGWVEQANGKKIPFSLNLEMKEGMSGSIRNEITYKSLENLGII"
                     /name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
     gene            10986..11342
                     /locus_tag="MTS34_RS20155"
                     /old_locus_tag="MTS34_20155"
     CDS             10986..11342
                     /locus_tag="MTS34_RS20155"
                     /old_locus_tag="MTS34_20155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005194067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000269902.1"
                     /translation="MWTVITTDLFNEWLAQQDESTQEKVLAALVVLQQQGPSLGRPLVD
                     TVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAIVLCIGDKGGKKRFYKEMLDIADQQ
                     YELHLSTLGDQSNG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            11335..11637
                     /locus_tag="MTS34_RS20160"
                     /old_locus_tag="MTS34_20160"
     CDS             11335..11637
                     /locus_tag="MTS34_RS20160"
                     /old_locus_tag="MTS34_20160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001140622.1"
                     /translation="MAKTLQELLASRSPESQARIQQMADELLLETQLHLIREELEISQK
                     ELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAMGGKLRIDVELPTGKHIGFNV"
                     /name="helix-turn-helix domain-containing protein"
     gene            11630..11839
                     /locus_tag="MTS34_RS20165"
                     /old_locus_tag="MTS34_20165"
     CDS             11630..11839
                     /locus_tag="MTS34_RS20165"
                     /old_locus_tag="MTS34_20165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000069473.1"
                     /translation="MSNNKPLKNSTKLLVNLDKFIFLVNAADSLEEIEIIRDLCCDYFS
                     NCKRSSYYIDIFDDAYWIKYYEIA"
                     /name="hypothetical protein"
     gene            complement(12165..12626)
                     /locus_tag="MTS34_RS20170"
                     /old_locus_tag="MTS34_20170"
     CDS             complement(12165..12626)
                     /locus_tag="MTS34_RS20170"
                     /old_locus_tag="MTS34_20170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(12931..15345)
                     /gene="znuD2"
                     /locus_tag="MTS34_RS20175"
                     /old_locus_tag="MTS34_20175"
                     /pseudo=""
     CDS             complement(12931..15345)
                     /gene="znuD2"
                     /locus_tag="MTS34_RS20175"
                     /old_locus_tag="MTS34_20175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /name="znuD2"
     gene            complement(15473..15787)
                     /locus_tag="MTS34_RS20180"
                     /old_locus_tag="MTS34_20180"
     CDS             complement(15473..15787)
                     /locus_tag="MTS34_RS20180"
                     /old_locus_tag="MTS34_20180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            complement(15774..16061)
                     /locus_tag="MTS34_RS20185"
                     /old_locus_tag="MTS34_20185"
     CDS             complement(15774..16061)
                     /locus_tag="MTS34_RS20185"
                     /old_locus_tag="MTS34_20185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438826.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            16370..17197
                     /locus_tag="MTS34_RS20190"
                     /old_locus_tag="MTS34_20190"
     CDS             16370..17197
                     /locus_tag="MTS34_RS20190"
                     /old_locus_tag="MTS34_20190"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057689930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
                     /protein_id="WP_000713530.1"
                     /translation="MKKFILPIFSISILVSLSACSSIKTKSEDNFHISSQQHEKAIKSY
                     FDEAQTQGVIIIKEGKNLSTYGNALARANKEYVPASTFKMLNALIGLENHKATTNEIFK
                     WDGKKRTYPMWEKDMTLGEAMALSAVPVYQELARRTGLELMQKEVKRVNFGNTNIGTQV
                     DNFWLVGPLKITPVQEVNFADDLAHNRLPFKLETQEEVKKMLLIKEVNGSKIYAKSGWG
                     MDVTPQVGWLTGWVEQANGKKIPFSLNLEMKEGMSGSIRNEITYKSLENLGII"
                     /name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
     gene            complement(17455..17991)
                     /locus_tag="MTS34_RS20195"
                     /old_locus_tag="MTS34_20195"
     CDS             complement(17455..17991)
                     /locus_tag="MTS34_RS20195"
                     /old_locus_tag="MTS34_20195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_005249161.1"
                     /translation="MKKLSVSELAKLYGYSRQAVYAHIKKGNLSKGSDGLIDFSEALRV
                     FGEPQKSNSSVNQSQSTSRHNLTEVDLLKRQVDMLEKQLNQAIQRENQSLERESFYQEQ
                     IEAMQRLLETPKANMTTFTDHESEQDIATDSRPQTESNYDGLTTPENKRIPVPEHIEPT
                     PKKRGFLSRFFLPYG"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(18059..18982)
                     /gene="repM"
                     /locus_tag="MTS34_RS20200"
                     /old_locus_tag="MTS34_20200"
     CDS             complement(18059..18982)
                     /gene="repM"
                     /locus_tag="MTS34_RS20200"
                     /old_locus_tag="MTS34_20200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282204.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_004282204.1"
                     /translation="MKTELIVKDNALINASYNLDLVEQRLILLAIVEARESGKGINAND
                     PLTVHAESYINQFGVHRNTAYQALKDACDDLFARQFSYQSLSEKGNVINHKSRWVSEVA
                     YIDNEAVVRLIFAPAIVPLITRLEEQFTKYEIQQISNLTSAYAVRLYEILIAWRSTGKT
                     PLITIYDFRQKIGVLETEYKRMYDFKKYVLDIALKQVNEHTDINVKVEQHKTGRSITGF
                     SFSFKQKKSATNTANDISQDKELKINLTDAQRYLFASKLSELPEMAKLSQGNESYEQFA
                     ARIVTMLQEPNKLKEFMPLLRKVGFQ"
                     /name="repM"
     gene            complement(19508..20944)
                     /gene="mobQ"
                     /locus_tag="MTS34_RS20205"
                     /old_locus_tag="MTS34_20205"
     CDS             complement(19508..20944)
                     /gene="mobQ"
                     /locus_tag="MTS34_RS20205"
                     /old_locus_tag="MTS34_20205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005248576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_039090895.1"
                     /translation="MAIYHFSVKTVARSAGRSATAAIAYRAGEKIYCEREGREHDYSRK
                     TGVEYKEIYLPEGAPEYLKNRGKLWNEVEQRETRKNSTVAREFEIAFPSELNQEQRLAM
                     LEELCASIVDRHQVAVDACIHAPHTGSGSDERNYHAHILMSTRKLTPEGFTEKTRELDQ
                     KHSGEIEHWREHFANICNMHLDLAGSTARVDHRSYKDQENGLEATLHEGPKVTELRRKG
                     IETEISRSNDEIKQRNQAQLQYGKNMDLLIAENEIKLRKLKTEQQIQIKNSAKTPPIDE
                     KALFEEKQRETLVKLLQGEISAKEANLDLHFMQDVFKKAENTLGKMQKHKNEFNQQLAQ
                     EIVKSGLKQSHDKLQSLVDQHNQLTQNKPLLFGKKAWEAQRDAIYQEHKKLKGQHEHQK
                     KHGVKDLLENEKFKEHAWKQYQQQHPAKAKQYQNLYSTYQVIKKCVNEIKAEQQLKLRQ
                     EQQLKAQQHAPKMKSRGMSR"
                     /name="mobQ"
     gene            21155..21421
                     /locus_tag="MTS34_RS20210"
                     /old_locus_tag="MTS34_20210"
     CDS             21155..21421
                     /locus_tag="MTS34_RS20210"
                     /old_locus_tag="MTS34_20210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_088749624.1"
                     /translation="MSVTETLDSKIKAQEEKLKQLKAQRQAALARERAKEKEQARKDDT
                     RRKILIGSCMLKITEDDEQARAKLIAQMDKYLTDERDRKLFNL"
                     /name="mobilization protein"
     ARG             complement(1823..2703)
                     /name="mph(E)"
                     /gene="mph(E)"
                     /gene_name="Mph(E) family macrolide 2'-phosphotransferase"
                     /tool="amrfinderplus"
                     /drug_class="MACROLIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             16371..17194
                     /name="blaOXA-72"
                     /gene="blaOXA-72"
                     /gene_name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(9554..10377)
                     /name="blaOXA-72"
                     /gene="blaOXA-72"
                     /gene_name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             5489..6312
                     /name="blaOXA-72"
                     /gene="blaOXA-72"
                     /gene_name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(2760..4234)
                     /name="msrE"
                     /gene="msrE"
                     /gene_name="msr-type ABC-F protein"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; streptogramin
                     antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(1820..2703)
                     /name="mphE"
                     /gene="mphE"
                     /gene_name="macrolide phosphotransferase (MPH)"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(7230..8179)
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(18060..18982)
                     /name="rep_cluster_1254"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.784"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(19512..20944)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.351"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagttaatac gtaatacaga attattcttc gaacatcact ggaatgtaga cctattagga
       61 aagcctcctg aatggtcacc agtacatttt aattttggaa aaattcctaa ttacgataaa
      121 caaggtgtat atgcgtttgt caaaggggat ttagtgactt atatcggtgt tgggacttct
      181 agaggtgctg gccgttatcg tggtaatggc ttaagcgcca gggtaatgaa atattgccga
      241 tggaatgaag ataaaactga atatattgcg atagatccaa gactgaaaga tgctggatcc
      301 atcattacaa taggctttga gcgtgatacg gcttatttag caaatgccct agaagttttt
      361 cttatctcta aaatccaacc tgtccacaat gtggttaaag ttggtatgta atttcgcata
      421 agaaatttta tgttaaatgg attcaatctc tgtcatcaaa gtcagcatta attctatcga
      481 cagtccattt tactgtattg ttatctaagt aaaaaaaatc tctatttaaa atatcatctt
      541 caaaaattaa gccattaaca tctagcttcc cttcttttaa atatccaata atttcatatc
      601 ttagactatt atctatttgc ttaatttgat gggaataatg tgttttctca ataaggtaat
      661 catcaaaaat agtagcttct aaatctataa ggtaattttt tttgagatca atacatgagg
      721 gtattgaaga actaaatacc tctagaattt cattacgaac cttaattttt agaatgaatt
      781 cgagattctc attacattct atgatttgag ctatatattt cacttcgatt aagatttcct
      841 aaaattaaca taatacacgt tatacgaatt cccaaggcat ggcttttagt catgcttttt
      901 ttatcaaaag actatacctg aatgatagcg agccaaatcg catacaatat ctatcattag
      961 acttaatgca ataattaaat gatagtatta tcattaatct aatagaatta agattaaatg
     1021 atagaaatga atacacgtat ttatctacga gcttcaacta aagatcaaga tgcagaaagg
     1081 gctttgcaga ttcttcagga tctaaaccaa aacttgaatt tgggtgaaac cattgtgtac
     1141 gtggaaaact atagtggtac gaagttagac cgacctgaat tgaataagct actgtcagaa
     1201 gcaaatcaag gtgacacatt gttagttgaa agtattgacc gcttatcacg cctaacccaa
     1261 cgagattttc aagagctgaa gcgcaggatc caggagaagg gacttcgttt agtcgtagca
     1321 gatcttccta ctacctacca aatgattcaa accagtgaca gcattactca ttcaatcttg
     1381 gaactcatca acaatatgtt gattgatctt ctggcaacaa tggcccgcct agacaatgag
     1441 aaacgtatag aacgtattaa gcagggcctg gcacgttcgg gttacaaacc aacaggcaag
     1501 aaggcaaatg aggctaaaca taaacgaata aaagaattgc tagtagttgg caatatgact
     1561 aaggaagaaa ttgccaaagc agtgaattgt ggagttgcaa ctgtctatcg agttgctaaa
     1621 gttatctaaa agaggcttat ctcaacgcgc ccccaatggg gcaccgctgc attcaggtta
     1681 cttcacataa gaaattttat gttaaatgat tgttagaaat gccccttgat cgaggcattt
     1741 tctataaaga atactaaaaa ctaattaaag ccaaccaagt ttcaaaacaa ggcagttctc
     1801 agcaatcata tttttaaatt atataactcc caactgagct tttgctccaa cgataaggct
     1861 ttcattttga gtttctaagg caaataaacc atacatcaaa ggagaggccg ctgctctttc
     1921 taaagtctgt tcatatagtt tattccaaac tttaccccct agtttttcat actcgatgat
     1981 tagagttttg aggctttctt ctccaaacaa agttacatgc ccagcaaaat caatcgctgg
     2041 gtcatctata tgggctgttg accaatcaat aacgcctgaa acagctccat cctttgaagc
     2101 tagtacatgc ccagcatata aatcgccatg tataaattgg gtgaaatctg cccatagaac
     2161 atcattatcc aaccattttc tgtagcgggt ttccaattgc tcacttatac caatttcaga
     2221 ttttactaac tgcaaattgt ttgctatttc aggtcttaaa tctgaaggtt tcataatttt
     2281 caaatcattt tcccgaactt ctttttcagg aatactatgg atttcaaata aggtttttgc
     2341 caaagatgtt atgtatttcg ggctatcttt gtccatattc caaattattt cataggtttc
     2401 agcatccaaa tttaaaacag gattatcttt aagtatggga taagccacta attctgtaga
     2461 tgaaattctc caatcaggaa cctctacaga aagatgtttt tttaccaatt ctaaaatgcg
     2521 tttttctttc ttgatttgtt ccctcatgcc atcacgacga ggaatacgca gcaaccattg
     2581 ttgccccttt gtatcaagag caaaaacgac cttaaaatca atgcccattt cattgaaatt
     2641 cattttgtcc gtaagcaaca agccgtgtgc ttcagcaagt gattgaatat cttgaattgt
     2701 catttttaat ttcctttaaa gagttcaata attaatgttc ggattagatt ggctatcatt
     2761 aacaatctct ctcaaaagtc ttgatgattt tgtggtcttt gatctcgtag ataatgtcag
     2821 caatattatc gaccaattgc ttgtcatgag atacgaagat aatagttcct gcataggact
     2881 tcatcattgt ttctaatgcg gcaatacttt ttaggtcaag atagtttcct ggttcatcca
     2941 taagcaaaat attatatttt cctaaaagca ttttggataa aagcagtttg atgatttcac
     3001 ctcccgataa gtcggataag tttttttgaa tatcattcgc tccgatcccc attgaagcca
     3061 atactgcacg aatttccgca actgtgtact cgcactcttc ctgcataaag gagagcacag
     3121 atttatgcgt gttaaattta tatcctgttt gtgtaaagta gccaatttca gcttttggag
     3181 atatggttaa tccatcagca cgttctgata tcatttttaa caaggacgtt ttccctgttc
     3241 cattcgatcc agttatagcg actttagcgc caagcggtat tataaagtta gcgtcatcaa
     3301 agatagtacg gctaccaaat tttaagctca gaccatctgc cgtaatcggg aacttattgt
     3361 gcagttctag ggctgaactt tgacgaaaac gaatagaacg caaatgctct ggtgcttgaa
     3421 tatcttctaa tgcagccaaa cgcttttcca tactcttagc tgcctgatac agttttcttt
     3481 gcttggtgcc agtcattttt gcatgcccaa gtcgtccagc actttcggta gagtttttgg
     3541 atttttctcc ttttttctta ttgtctaatc gattagcttg ctggcgtttt tcttgcacag
     3601 cagattctaa tcgctcccgt tccttcatca tcagctcata ttctacggct tggtgttgtc
     3661 gctcttcttc tttttgacgc aagtaatccg agtaaccacc ccaatattcc gtaattttac
     3721 cgtcttttaa ctcccatatc ttgtctacaa ccatatcaag aaaatatcgg tcatgactga
     3781 taacaagtaa tgctccatca aatgctttaa gttgaccaat aagtagatct attccattga
     3841 gatcaaggtg gctggttggt tcatccgcta gaatgccatg tacttgttgg gaaaatgcgg
     3901 cagcaatttt tgcacgagtt tcctctccgc cactcattgt gtcgttttgt acattggaaa
     3961 caccaaggcg agataacatt gcccggtctt cgaccgtttc tatttcgatt ccgcccagtt
     4021 ggctgatatg tgcaaaatca ccaaaacgct gtaatgtcgc ttcggctaaa acaatttcgc
     4081 cattaagtac tttgagtaaa ctactctttc ctgctccgtt atcacccaca agaccaatac
     4141 ggtcataaga gtgaatttcc aattcatcaa tatccaaaac atcacgccca gcataatcca
     4201 agcgtatgtt tctcgcttta ataattaaac tcatttttat ttactcctgt ttagctcttg
     4261 aaatttttta tgcagcaaac aggatttagg tgaaaacaaa agctagcatc acagtgtcct
     4321 cccaaaaaaa aagctattca tccacagggt ggacaaatag ctagtcaatt aagttataac
     4381 tggaaactat gcactaaaag catacttata aattaagcat acttttactt tatatatccg
     4441 catatattct taaagacaca acaaaagccc accattataa aatagtgtca ctatgcaaat
     4501 agttgtgtct taacgaatgc ggatagaatg cataaactta cctaaaaaat aaaattcagt
     4561 tttatgataa ctttactgtt aaaagaagtc tagacaacct tgttttatgc ttggatataa
     4621 ggcttatttt aaaataatag tagtgaagat tttctaaaat taacataata caccttatac
     4681 gaaataaaaa tgggagcctt aagctcccat tttttaagta attttttcaa ataaggcttg
     4741 ggtgagcatc taaaattcga atcaatgtag ctgctggtcc agttggatag cgtcgtccct
     4801 gttcccaatt ttgcagggta cgggggctaa tatgtagacg tgcagcaaat tcattttggc
     4861 tcaagccagt tttttcacgt acttctttaa tatcaggcaa ttcgagttct gtaactcggc
     4921 ttgctttaac ttctttacgt ttgatagctc cagcttcttt gattgaagca accaagtcat
     4981 caaataagtt gttatccatg aaattgctcc ttcacgagtt ggtgcagaat ggcggtttcc
     5041 ttatctgtta aattatcttt tactgatttt ggataagcca ctaagaaaaa gatctgatca
     5101 tcctcggtga cccaataata aatcacccgt atcccaccac ttttcccttt gttaccttga
     5161 gcacaacgta ctttgcgaat accaccgcca ttcttgatta ggtcacctct atcaggctgt
     5221 accaaaagat cttgctgaag ttgacgatat tcctcatcag atacaaggtc tttaatttgc
     5281 ttggtaaaga tgctggtttc aataaataac atggactctt tatacgtcaa tggcgtattt
     5341 gaattttagc agtttccata acaaaaaacg agagctggta gatctcgttt cgcataacgc
     5401 ccattatgtt aaattaaaag agttttaact tttttaataa aaatattatg ctctaagccc
     5461 caaaatttcc cctaacatga atttgtaatg aaaaaattta tacttcctat attcagcatt
     5521 tctattctag tttctctcag tgcatgttca tctattaaaa ctaaatctga agataatttt
     5581 catatttctt ctcagcaaca tgaaaaagct attaaaagct attttgatga agctcaaaca
     5641 cagggtgtaa ttattattaa agagggtaaa aatcttagca cctatggtaa tgctcttgca
     5701 cgagcaaata aagaatatgt ccctgcatca acatttaaga tgctaaatgc tttaatcggg
     5761 ctagaaaatc ataaagcaac aacaaatgag attttcaaat gggatggtaa aaaaagaact
     5821 tatcctatgt gggagaaaga tatgacttta ggtgaggcaa tggcattgtc agcagttcca
     5881 gtatatcaag agcttgcaag acggactggc ctagagctaa tgcagaaaga agtaaagcgg
     5941 gttaattttg gaaatacaaa tattggaaca caggtcgata atttttggtt agttggcccc
     6001 cttaaaatta caccagtaca agaagttaat tttgccgatg accttgcaca taaccgatta
     6061 ccttttaaat tagaaactca agaagaagtt aaaaaaatgc ttctaattaa agaagtaaat
     6121 ggtagtaaga tttatgcaaa aagtggatgg ggaatggatg ttactccaca ggtaggttgg
     6181 ttgactggtt gggtggagca agctaatgga aaaaaaatcc ccttttcgct caacttagaa
     6241 atgaaagaag gaatgtctgg ttctattcgt aatgaaatta cttataagtc gctagaaaat
     6301 cttggaatca tttaataatt tctaaaatta acataataca ccttatacga aatgctgtat
     6361 aattatcctt ataattcaac gctgtagttt ctagtttata gcccgagcat ccctgttcgg
     6421 gcttttttta tgacttttca cgttccacgc tattatttca tcaatgttcc acacttttga
     6481 ttagctcaat tgaataatca ggatgcctgg tctttttctt tttggcattc atccagctct
     6541 ttcttcagcg cctttttatc ctggtacgat gcaatcagca tgatcgggat cagcagcgga
     6601 gctacaataa ttcccagggc aatgtctttc agtttcggca tgatctttca cccttattgc
     6661 tcagtcatta tcaaagacgg cattcagcac acggccaaat aaaccacgtt ttttgtgttg
     6721 tggcactgcc acactttgca acgtggcatg ctgtagcgtt tgggtgtggt atgccgtagt
     6781 ttcttgctgt ggcactgcta cattttctgc aatatttaat tcattattct ttttgttatc
     6841 agtgtcttgt tgtgttgtcg catgctgtgg catcttggtg tcgcatgccg tagcatcttg
     6901 gtgtggtgct gctgtggcag gtgcaacact tttcagctca atgagtgttt gcatacggtc
     6961 aatttgttgc attaaccgtt cttcacgttc ttggtatttt ttgacttcat tttctaaatg
     7021 ggcaataagt ttttttgtag attcatcacc ttcttgctta gatgattcaa ttttttgaga
     7081 tgtttgctca ggttctccaa agcatcgtat gacctctgct agatcgaatt gtccatcact
     7141 ggagcgtgat aaacgtccat tacgtacagc tctgtaaatg gttgatctat ccacatgaaa
     7201 ttttttagat acttcactta gtgataactt agccatttac ggtgtccttt ttatttgatg
     7261 gcatgtatcc cacttttttt aggtatgggt atagctcttt gaattgagcg ggatcttgta
     7321 gcatctcagc aatacgtaca gcaaactgtt ggtagctttc tgttccttgt gaatagcgac
     7381 ccatttcagg gagttctgaa agtttatttg caaacatatg ccgttgagca tcggtcatct
     7441 ttgaaaaaag ctccaaagtg ttcggatctc tttctattga tgggctatcc ttcttcttct
     7501 gtttaaaggt aaaagaaaat cctgaaatag aacgtcctct tttatgctgt tcatatttta
     7561 cagttatatc cgtatgctca tttatttgtt taattgaaag ctctaaaaca cgctctttta
     7621 aatgagccat ccgtaaatat tcattatcaa gaacgcctaa cttctttcta aattcttgta
     7681 gttctatggt tggggtttta ccagtgcttc gccactgaat tagaagctca tataagcgaa
     7741 tggcataagc gctactaaga ctactaactt gctgtaattc atatttagta aattgttctt
     7801 ccagtcgggt tatgaaggga acaacagcag gtgcaaatat taagtctaca gttgcttcat
     7861 tgtcgttata agcaatttga gatacccaac gactcataac atttcggata ttcccattag
     7921 cttttttctc ttgatagcta aattgacgcg cgaataaatc cttacaagca tcttttaagg
     7981 cttgataagc tgtattacgg tgtacaccaa attgattgat ataactatct gcatgaactt
     8041 ctaatggatc atttgcatta atccctttcc cactttccct tgcttcaaca atagccaata
     8101 aaattaaacg ttgttctact aagtctaagt tatagcttgc gttgattaag gcattgtcct
     8161 ttacaactaa atctctcata gccactattt tttattgtgt ataagtacac gttaatatta
     8221 tttgtgtact aatgcaaggt aaacgtggac atattgcaag gtaaacgtgg acatattgca
     8281 aggtaaacgt ggacatattg caaggtaaac gtggacatat aagagctaaa accctttatt
     8341 cataaggctt tcaagccatt taaaagtatt taaaaacttt aaaataatta aaagcccagg
     8401 gaatgaaaaa ttgcccttgg ttttcgcttc gctcaaactc tattgaactt cgctttcgct
     8461 cagttcgttg gggcaatttt ttggttaata ctggcgtgac tttaaaaaaa agcaaaagaa
     8521 acttggaatt cgcttcgctc ataaaacttt tttactcgct acgctcgatc caggagcaaa
     8581 tttctgcacg aaacttacgc tttcttaggc taaagaggac aaaaaaatgt ttttcagagg
     8641 gtctaggcgc aaattttgat agttcgctcg tagacactcg ctctatgcat ctatctaaag
     8701 tcaaaatacc tctttatttc gctctcattt gcgttttttg agctgttttg tgtttttcac
     8761 tatgagatct atgttttcta ggtaaaggct cttaaatcgc atttgaacgc gttctatgaa
     8821 gcttttcata attaaagttt tgggttgaaa tttttaacct ggatgaatgg acaaaaaatt
     8881 gattttttac gtttttctaa acaaggaaat aaatactttt ttccatgcaa aaccaagcac
     8941 cggccctacg tcactttagc caattttcca atccaagatc cggcacatgc cgggattttt
     9001 ttgtcacaca ccacgcatct aaagatgatg accttcaaag ctttatttca taaggttttt
     9061 ggttgttttt ataggttctg aattgtgtac aaaataattg atctttttaa tactaatttt
     9121 ttgatttata tagtttttag tatgtacata gtgttgtaca taaattaatg gttttttagg
     9181 cttaaatata tgaaatttat aaaaaagagt ttgtacacat ggattataca agaatgtaca
     9241 aagtttaacg tgaaattagg tataacatac tgaaaaatat tgaaattaat atgtacaagt
     9301 tgaggataaa cattatgtca aaagaattag tcaacttagc aaatatcgtt ccttttccta
     9361 aatcatgtgg attctgtgaa tcaaaggaaa atgtacaact ttttgcaggg ttaatgcttt
     9421 gtagtagctg tcaggaaaac atccgaacca ctaatcctgg aatgtttaca gctaataagc
     9481 aaattgaaaa aaaagcccag gattaatctg ggcttcgcat aaggtgtatt atgttaattt
     9541 tagaaattat taaatgattc caagattttc tagcgactta taagtaattt cattacgaat
     9601 agaaccagac attccttctt tcatttctaa gttgagcgaa aaggggattt tttttccatt
     9661 agcttgctcc acccaaccag tcaaccaacc tacctgtgga gtaacatcca ttccccatcc
     9721 actttttgca taaatcttac taccatttac ttctttaatt agaagcattt ttttaacttc
     9781 ttcttgagtt tctaatttaa aaggtaatcg gttatgtgca aggtcatcgg caaaattaac
     9841 ttcttgtact ggtgtaattt taagggggcc aactaaccaa aaattatcga cctgtgttcc
     9901 aatatttgta tttccaaaat taacccgctt tacttctttc tgcattagct ctaggccagt
     9961 ccgtcttgca agctcttgat atactggaac tgctgacaat gccattgcct cacctaaagt
    10021 catatctttc tcccacatag gataagttct ttttttacca tcccatttga aaatctcatt
    10081 tgttgttgct ttatgatttt ctagcccgat taaagcattt agcatcttaa atgttgatgc
    10141 agggacatat tctttatttg ctcgtgcaag agcattacca taggtgctaa gatttttacc
    10201 ctctttaata ataattacac cctgtgtttg agcttcatca aaatagcttt taatagcttt
    10261 ttcatgttgc tgagaagaaa tatgaaaatt atcttcagat ttagttttaa tagatgaaca
    10321 tgcactgaga gaaactagaa tagaaatgct gaatatagga agtataaatt ttttcattac
    10381 aaattcatgt taggggaaat tttggggctt agagcataat atttttatta aaaaagttaa
    10441 aactctttta atttaacata atgggcgtta tgcgaaatca atttaaatta ttttaattaa
    10501 atcaaatatt tatgtattct acataacgcc aaaaaatacc agaatgccga ctcggaaaca
    10561 agttgggtat tttttaagct tctttgtcac ttttagccaa taaaatagat taaaaaatat
    10621 tcaaaaattc agttttcttt gatttctcaa cagtcagcat tttgaaataa tccacaaaat
    10681 ttgcggataa atcttgggat aaatatacgc caattatgta cgcctaagcg cacaccgatt
    10741 gagggtatta accaatagtt agactcagct taattttcta tcatggacac atagagagca
    10801 ggatgctcca gcacacaaaa caactatcat aagtgagcgc actaggatcg tgaagcaaag
    10861 tagaagttac acttgagatg caaaaacgaa aaagccagac aggatgtcgg gcttttttat
    10921 tgtcatttga ataataattc aattaagcat aaaatataga ctgtaatcta tattatggtt
    10981 gtaatatgtg gacagtcatt acaacggatc tgtttaacga atggcttgca cagcaagatg
    11041 aatctacaca agagaaagta ctcgcagcgt tggtcgttct acagcaacag ggaccaagtt
    11101 taggtcgtcc tttagtagat accgtctatg actccaaatt taccaatatg aaagagctac
    11161 gtgttcagca tcgaggtaaa ccactgagag cctttttcgc atttgatcct ttacgacaag
    11221 ctattgtgct gtgtatcggt gataaaggcg gtaaaaagcg tttttataaa gagatgttag
    11281 atattgctga tcagcaatac gaacttcatc tctcaacttt aggagatcaa tctaatggct
    11341 aagaccctac aagaattatt ggctagtcgc tctccagaga gtcaagctcg tattcaacaa
    11401 atggctgatg aattactgtt ggaaactcag cttcacctta ttcgtgaaga gttagagatt
    11461 tctcaaaaag aactggctgc aacccttgga atcaaacagc cgtcattgtc agcaatcgaa
    11521 aatcgtggtc atgatcttaa aatttcaacc atgaaaaagt atgttgaagc aatgggcgga
    11581 aaacttcgta ttgatgtaga gcttccaaca ggaaaacaca taggatttaa tgtctaataa
    11641 taaacctttg aaaaatagta ctaaattact tgttaacttg gacaagttca tatttttagt
    11701 caatgctgcc gatagcctag aagaaattga aattattaga gatttatgct gtgactattt
    11761 ttcaaattgt aagaggtcaa gctattacat tgatattttt gacgatgctt attggataaa
    11821 atattacgaa atagcttaat ccttccagat tcgcataaga gattttatgt taaatgccga
    11881 actgaattaa aaatagaaat gtagttgagc tgatgcttgt tcatttcttt atttttagta
    11941 attgaggtga acatactgtc agtacatggt agttataagt aacgtgtaac tttgtttgca
    12001 caaagatttg agttatgcca agtgatggat tgatttgtac ttaaaatttt aagcttatcc
    12061 acttgctttg caagccacta cactttattt acaatcctat gttgtggaag taagggatct
    12121 tgtactcgcc cagtatgttc aacttgatta tatctaaagg actattaggc ttcaatcaaa
    12181 tcaattttcc aattagtcga ctgaattaac acgtttaaat cacgtaattt aagactaata
    12241 tcatcagctt gtttttgtaa tgatgaaact ggaataactt tatgccactt tatttcccgc
    12301 gtactataac gatcagattc acgagatgta ttacttattg ctgcagttag ggttttgtgc
    12361 tgttctacaa attcatcacg caaagtcaat aaagcaagta gagaacggcc atcatttaat
    12421 tttgttaaag cattagtttc atgaattgca taacttaatt tttgaaattc ttgaattaaa
    12481 gaaaatactt gttttagtag ttcatttgga tcttcagatg gttgatcacc atcttgtacc
    12541 aatacatttg cattgatacg ttgttttagt gaaataattt ttttttgttg atcacttcga
    12601 agtaagagag cttctgctaa tttcattttt cacctattaa ccaattagaa ttaagtttta
    12661 aacagcttat atcctcattc tttaaagatc aaaaaaattg ctttttatct agctaaaagg
    12721 tgtctacaaa atcggtggct attcagtgct gaaaaggctg ccgacacata aagtgaccca
    12781 gattgaagag ttactgccac actgctggaa acctaaatcg aattaaaaaa taggtatggg
    12841 attcagcgga cgcttacctt aattattaat ctaatcattt tataagaaaa atcttctaag
    12901 aaattttctt agaagattac agtatcttat ctagaaatta agatttaagc ctaaactaaa
    12961 atttcttcca atttggggaa tatatggcaa aaatgtttca tgggcataga ctttctgatc
    13021 aagtaaatta ttcgctttaa agaataaatc atattctgtg tgagatagct tatttttata
    13081 tgctaaagtc atattaagca tattataacc tgatgtaacc tgctcaaatt tagaaatatt
    13141 atcttgttta aagactcgat aatattctat ttcaccttta agcgattcat caaaatcagc
    13201 ttttagtcgt gtacccaaac gagcaggagg taatctagga gtgtaacgat ccttttgggg
    13261 agctaaagtc ggttctctat tccagatatc gtatgcaatt acagcatcag gtaaatttgt
    13321 taaacggcct ttcacataat ctccaaaaag agagccgtga taaacagaat taaattgata
    13381 ccctatgttg ccctctaatc cataaaaatt agcagcagac tggctataat gattaatacg
    13441 tagtgtatgc tgatctctaa cttttgtagt tcctaaaact tcattgagtg tctgtaaata
    13501 tatatagtta tcaaaatcat aatagtaagt actgatttga taatcaagta aatcatcctt
    13561 ataggccaga ctgatttcta aattattgga tttctctttt cttagaaagc ggttacctat
    13621 ttcaaaagaa ttagtagcga gatgcatacc atgagtatac atttcttgtg cattaggtaa
    13681 tctttcttga tgtgtacctg ttaatgataa gatataattt ggagcaaatc tccagtggac
    13741 tgtacctgca taagaaaaag ctgtttcttt gttaggttga acagcttcta aaatagattt
    13801 taaattttga gccctgattt tattattttt ttctacataa ggactgttat atttattagg
    13861 ccaaggtttc attgaatctt taattttttc tatatcataa tccatactaa ctttctgctt
    13921 ttctatacga gcacctagtt cgaaagtaaa gtcgttccag ttatattttt ctaggccaaa
    13981 tacactaaag tttttagtga catttcgatt taatagctgc tgattatcta gataggtggt
    14041 atggccttct tgagaatgga ctggtgataa agcactatta tcctgttcca aatattgtag
    14101 ccccaagata ccggttaatt cccctaatgg ctgatgtgat aactctatgc ggcctacttt
    14161 tcctgtattt ttaaaaaagt tagtaatctt atctccctcg agttcattgt gaaaataatc
    14221 tatataactc aatgaggtac gaattttgtc aataccggta aatggttgag tgaattctcc
    14281 acgcacatca taacgtcgag tatttaaatc aatataagga gcattatgag agaaattagt
    14341 ttcatgaata tgattatgct gatgacaatc aggacgtgga tttatgtaat taatatcttg
    14401 ctcatccatc aattgaggat aatataaaag ataatttttt aatccactaa ttcgtgaatc
    14461 gataccgatt gcagaggcac cacaaccatt catataaatg gctatgggct ggtaaacccc
    14521 atattcatca tgacgatgtg tataagaaac tcctaaataa gtattatcgt ctatccagga
    14581 taatccaagc gtacctactc tcgactctgc ccagctttct ggtaaatatt ttaaattttt
    14641 ataaatactg ttttggtttt caagatcttc taaagaatta taagagctag actggtaatg
    14701 aggagtttta tagttaccag cattacgata taacccttct gcccgtaaag ctacatgtgg
    14761 gcttaagcca actgttacac ctgctgtggt caacttctca ttactgccag tattaaaacg
    14821 taatgtagta ttaccctcta atccgtcttg aggcatttgg gttggaattt tatagtcaat
    14881 gacattgact acccctgccg cactatttga actatacaaa agggtacttg caccacgtat
    14941 aatttcaata gacttagcta aacttggttc aactgtgaca gcgtgatctg gggacatatt
    15001 agacatatcc aacacgtcag cattattctg taaaacttta atacgttttc cttcctgccc
    15061 acgaataata ggagtagatg ctccacctcc atattgatta gaatgaattc ctaattccgt
    15121 acccaaggca tcccctagag ttgtcgaacg ctgtttaagt ttactagtat caataaactg
    15181 attactaaca tttagacttt tgcctaacag ctcagaccct aactcatcag attgtgcagt
    15241 taatactatt gtatttaagg tagtaacagg cccttcagct gccaataaaa ttttcgaggc
    15301 ccctaaagta attaaagcta aaaatagttt acttttattt agcataaaac aaccaaattt
    15361 aatgttatat tataatataa cacttaaaaa tttataaaat ctactattta aatttttgaa
    15421 tggtaatccc ccctaaaaat gaagtcaaaa atgggagcct aagctcccat tcttattgat
    15481 gattttttaa ttcttttagc atagcatctc gtaaaatttc attcatacgt gtttgataac
    15541 ctttgccttg tgctttgaac caagcaagta catcagcatc taaccgaatt gaagtttgtt
    15601 gctttactgg gcggtaaaat tgattttggc gtacagcatt gctccagtct gtaatttcag
    15661 gaatatctga tagatctagc tgatcatcag gaaccgtgcc tttagcaagc aagcgttgaa
    15721 tttcagcatc ttgcttctca ccaaatttct cattcagctc tttgtgtgag tatctaacca
    15781 tgctcatatt tgtttcgctc cgctttagtg acttgccttg cactaatgat tcggatgatt
    15841 tcacagtcat cttcatcaaa aatggtgtga gccaccaata acattagtac gcctttaact
    15901 cgtccaatgg tttgccaacg ttcttcacca ttggtatgtc tgtcttggat tgagatccgt
    15961 aatggatctt caaaaacaag gcttgcagtt tcgaaagaga tatcatgctt tttctgattc
    16021 tttcgattct tagcctcatc ccattcaaaa tactgttcca taaaaaacat tcaaatttgt
    16081 atatacaata atgtatcaca aaattgtatc actcaaccag attcgtgtaa tttttagtca
    16141 aaattattgg caagaagtat caaaactgct cataaaaaag ccgacttgtt tcaaagtcgg
    16201 ctttttgggc attttgggat ttttctattt ttattaagta gttgatattt atattaatat
    16261 acaagtgtac ttcgtataat cgccattatg ttaaattaaa agagttttaa cttttttaat
    16321 aaaaatatta tgctctaagc cccaaaattt cccctaacat gaatttgtaa tgaaaaaatt
    16381 tatacttcct atattcagca tttctattct agtttctctc agtgcatgtt catctattaa
    16441 aactaaatct gaagataatt ttcatatttc ttctcagcaa catgaaaaag ctattaaaag
    16501 ctattttgat gaagctcaaa cacagggtgt aattattatt aaagagggta aaaatcttag
    16561 cacctatggt aatgctcttg cacgagcaaa taaagaatat gtccctgcat caacatttaa
    16621 gatgctaaat gctttaatcg ggctagaaaa tcataaagca acaacaaatg agattttcaa
    16681 atgggatggt aaaaaaagaa cttatcctat gtgggagaaa gatatgactt taggtgaggc
    16741 aatggcattg tcagcagttc cagtatatca agagcttgca agacggactg gcctagagct
    16801 aatgcagaaa gaagtaaagc gggttaattt tggaaataca aatattggaa cacaggtcga
    16861 taatttttgg ttagttggcc cccttaaaat tacaccagta caagaagtta attttgccga
    16921 tgaccttgca cataaccgat taccttttaa attagaaact caagaagaag ttaaaaaaat
    16981 gcttctaatt aaagaagtaa atggtagtaa gatttatgca aaaagtggat ggggaatgga
    17041 tgttactcca caggtaggtt ggttgactgg ttgggtggag caagctaatg gaaaaaaaat
    17101 ccccttttcg ctcaacttag aaatgaaaga aggaatgtct ggttctattc gtaatgaaat
    17161 tacttataag tcgctagaaa atcttggaat catttaataa tttctaaaat taacataata
    17221 caccttatac gaaatgcgtt ataattctct tgaaaattca acgctgtagt ttctagttga
    17281 tagcccgaac atccctgttc gggctttttt tgtgattttt cacgttccac gcctattatt
    17341 taagcaatgt ttcacgactt tattcagttt taataaacat catccggttt tttgaggagt
    17401 cattatttca tgtgcttgca ctgaaattgg ctcatcaaaa aagtggagtc ctggttaacc
    17461 gtaaggaagg aaaaaacgac tcaggaagcc tctcttttta ggtgtaggct caatatgttc
    17521 tggaacaggg atgcgtttgt tctctggagt agtcaatccg tcatagttcg attcagtctg
    17581 tggccgagaa tctgttgcta tatcctgttc agattcatga tcggtaaacg tagtcatatt
    17641 ggcttttgga gtttccagta agcgctgcat ggcctcaatc tgttcttgat aaaacgattc
    17701 acgttctaat gattgatttt ctctctgtat tgcctgattt aattgttttt ccagcatgtc
    17761 aacttgacgt tttagcaagt caacttctgt taagttatgc ctactagttg attgactttg
    17821 gttgacacta gaattgcttt tttgtggttc cccaaaaact ctcaaggctt ctgaaaagtc
    17881 aattaatcca tccgatcctt ttgataaatt tcctttcttt atatgggcat atacagcttg
    17941 tcttgaatat ccatataatt tcgctagttc tgaaactgac agttttttca ttatgtaaac
    18001 cagttttcaa atagtgttaa tagttgattg ttaactttac tatgtcaatt gacatcattc
    18061 attgaaagcc gacttttcga agtaatggca taaattcttt taatttattt ggctcttgca
    18121 gcatggttac aatccgagca gcaaactgtt catagctttc attaccttgt gaaagtttag
    18181 ccatctcagg aagttctgat aatttactag caaataaata tcgttgtgca tcagtcaaat
    18241 tgatttttag ctctttgtcc tgactaatgt cattggctgt atttgttgct gactttttct
    18301 gtttgaagct aaatgaaaag cccgtaatgg atctgccggt tttatgttgt tcaactttga
    18361 cattaatatc ggtatgttca tttacttgct ttaatgcaat gtctaaaaca tatttcttaa
    18421 aatcgtacat ccttttgtat tcagtctcga gtacacctat tttttgtctg aaatcgtaga
    18481 tagttataag aggcgttttt ccggtactac gccacgcaat caatatttca tataagcgaa
    18541 cagcataagc actcgttaaa ttgcttattt gctgtatctc atactttgta aattgttctt
    18601 ctaatcgagt tattaaaggc acaatagctg gagcaaaaat aagtctaacg acagcctcat
    18661 tatcaatata agcaacctcg cttacccatc ttgacttgtg attaatgaca ttaccttttt
    18721 cactaagact ttgataactg aattgtcttg caaataggtc atcgcaagca tcttttaagg
    18781 cttgataagc agtatttcga tgtacaccaa attgattgat ataactttca gcatgaactg
    18841 ttaatggatc attagcattt atccctttac ctgattccct ggcttcaacg atagctaaaa
    18901 gaattaatcg ttgttctact agatcaaggt tataactagc attaattaaa gcgttatctt
    18961 tgacaattag ttcggttttc atatgaaaaa tttatatttt taagtcaata agacgaggtt
    19021 atattatcaa ctcgcttaat gcaaggtaaa cctcgtttta atgcaaggta aacctcgttt
    19081 taatgcaagg taaacctcgt tttaatgcaa ggtaaacctc gttttataat ctctcaaagc
    19141 cttataaatt caggtttaca ggctatctaa aagcatttaa aagcttaaaa ataattaaaa
    19201 gcatatttca tgaaaaattg cccttggttt tcgcttcgct caaactctat tgaatctcgc
    19261 tctcgctcga ttcgttgggg caatttttgg ttaatcccta ggtaacttca ataaaaagca
    19321 aaagcaactc ggaattcgct tcgctcataa gctttttttc tcgcttcgct cggtctagga
    19381 ataaaacttg gaaagttcgc acccattcgg gatgctctgt agggtaggtg attaatggat
    19441 agggagcttg taatcaggga aaggaaaggg caaattttct cagttcgctc gtagacactc
    19501 gctctgttta acgactcatg ccacgagatt tcattttagg cgcatgttgc tgtgctttga
    19561 gttgttgttc ttgtctgagt ttcagctgtt gttctgcttt gatctcattc acacattttt
    19621 taatgacttg ataggttgag tatagattct ggtattgctt ggctttggcc ggatgttgtt
    19681 gttggtactg cttccaggca tgttctttaa acttttcatt ttccaataaa tccttcacac
    19741 catgtttttt ctggtgttca tgctgacctt tcagtttttt gtgttcttgg taaatcgcat
    19801 cacgttgggc ttcccaggct ttttttccaa acagtaaggg tttgttctgg gttaattggt
    19861 tgtgttgatc caccaggctt tgcaatttgt catgactttg cttgagtccg cttttcacga
    19921 tctcctgggc gagctgctga ttgaactcgt ttttatgctt ttgcatcttg cccagggtgt
    19981 tttctgcctt tttaaaaaca tcctgcataa aatgcaggtc tagattcgcc tcttttgcgc
    20041 ttatttcgcc ttgtagcaat ttgactaggg tttctctctg tttttcctca aacagggctt
    20101 tttcgtcaat tggtggcgtt ttagcgctat ttttgatttg gatctgctgt tcagttttca
    20161 gttttctgag ttttatctca ttttcagcaa tcagcaggtc catatttttg ccgtattgca
    20221 gctgagcctg atttctctgt ttgatttcat cattgcttcg gctgatttcg gtctcaatcc
    20281 cttttcggcg cagctcggtg actttcggtc cttcgtgcag ggtggcttcc aggccattct
    20341 cctggtcttt gtagctgcgg tggtcaaccc gggcggtgga cccggccaga tccaggtgca
    20401 tattgcagat attggcaaag tgttcgcgcc aatgttcgat ttctccactg tgtttctgat
    20461 ccagttcccg ggttttctcg gtaaaacctt cgggagtgag cttgcgtgtg ctcatcagga
    20521 tatgggcgtg ataattgcgt tcatcactgc cggatccggt atgcggtgca tggatacagg
    20581 catcgacggc cacctgatgt ctgtctacga tactggcgca tagttcttcg agcatggcca
    20641 ggcgttgttc ctggttcaac tcacttggaa aagcaatttc aaattcccgg gcgactgtcg
    20701 agttttttct ggtttctcgt tgttccactt cgttccagag ttttccccgg ttttttaaat
    20761 actctggtgc gccttcgggt aaataaattt ctttgtattc cacaccagtt tttcggctgt
    20821 agtcatgttc tcggccttca cgttcgcaat agatcttttc acctgcccga taggcaatcg
    20881 cagcagtggc ggaacgccct gctgatcttg ctacggtttt aactgaaaag tggtaaatcg
    20941 ccatgtctgt ttttcgcttt taaattttca cgtagtgaaa tacaaccgtg aatacggttg
    21001 tcggggtatt gggggagacc ccaatcgtac ttgcaaacaa agtttgcata agtgcgctct
    21061 tcgaggaaaa cattactata catatagcac cttgaatgca tattcaagtg cgcttatgtt
    21121 agggatggtt tcccaggatg atggtattgg attaatgagc gttactgaaa cactagacag
    21181 caaaatcaaa gcacaggaag aaaagctaaa gcagttaaag gctcaaagac aggcagcact
    21241 ggcaagagaa cgtgctaaag agaaagaaca agctcgaaag gatgatacca gacgcaaaat
    21301 tttaattgga tcctgtatgc taaagatcac tgaagatgat gaacaagcca gagctaaatt
    21361 aattgctcag atggataagt atttaacaga tgagcgtgac cgaaagcttt ttaatttata
    21421 aattttgagg gataagtagt ggaagatcca acac
//
