LOCUS       NZ_CP121600             8723 bp    DNA     circular CON 17-MAY-2024
DEFINITION  Acinetobacter baumannii strain JAB77 plasmid pI_RAB77, complete
            sequence.
ACCESSION   NZ_CP121600
VERSION     NZ_CP121600.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27010219
            Assembly: GCF_029714005.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 8723)
  AUTHORS   Alghorbi,M.F., Bawazeer,R., Okdah,L., Alswaji,A., Alzayer,M.A.,
            Alrashidi,E., Alshahrani,A.S., Al Johani,S.M., NGHA Surveillance
            Group,N.S.G., Balkhy,H. and Doumith,M.
  TITLE     Antimicrobial resistance and genomic characterisation of
            Acinetobacter baumannii clinical isolates from a nation
            representative surveillance in Saudi Arabia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8723)
  AUTHORS   Alghorbi,M.F., Bawazeer,R., Okdah,L., Alswaji,A., Alzayer,M.A.,
            Alrashidi,E., Alshahrani,A.S., Al Johani,S.M., NGHA Surveillance
            Group,N.S.G., Balkhy,H. and Doumith,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-APR-2023) Infectious Diseases Research, King Abdullah
            International Medical Research Center (KAIMRC), KAIMRC, P.O Box
            22490, Riyadh 11426, Saudi Arabia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: EToKi v. 1.2
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 131x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029714005.1-RS_2024_05_17
            Annotation Date                   :: 05/17/2024 05:04:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,982
            CDSs (total)                      :: 3,887
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 39 of 93
            Pseudo Genes (incomplete)         :: 43 of 93
            Pseudo Genes (internal stop)      :: 18 of 93
            Pseudo Genes (multiple problems)  :: 7 of 93
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP121600.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8723
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="JAB77"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pI_RAB77"
                     /geo_loc_name="Saudi Arabia:Jeddah"
                     /lat_lon="21.4858 N 39.1925 E"
                     /collection_date="2018"
                     /collected_by="KAIMRC AMR WGS_Based Surveillance Program"
     gene            join(7646..8723,1..1326)
                     /gene="znuD2"
                     /locus_tag="MTS78_RS19915"
                     /old_locus_tag="MTS78_19915"
                     /pseudo=""
     CDS             join(7646..8723,1..1326)
                     /gene="znuD2"
                     /locus_tag="MTS78_RS19915"
                     /old_locus_tag="MTS78_19915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /name="znuD2"
     gene            1631..2092
                     /locus_tag="MTS78_RS19920"
                     /old_locus_tag="MTS78_19920"
     CDS             1631..2092
                     /locus_tag="MTS78_RS19920"
                     /old_locus_tag="MTS78_19920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(2415..2588)
                     /locus_tag="MTS78_RS19925"
                     /old_locus_tag="MTS78_19925"
     CDS             complement(2415..2588)
                     /locus_tag="MTS78_RS19925"
                     /old_locus_tag="MTS78_19925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001282484.1"
                     /translation="MSDNIIQLSKPCTLCENRQNVQLFAGLMLCGSCQENIRLTNPSMF
                     SANDEIEQKAQN"
                     /name="hypothetical protein"
     gene            complement(2588..2959)
                     /locus_tag="MTS78_RS19930"
                     /old_locus_tag="MTS78_19930"
     CDS             complement(2588..2959)
                     /locus_tag="MTS78_RS19930"
                     /old_locus_tag="MTS78_19930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000504218.1"
                     /translation="MGEKVKTYAVRLDPQVAEFYDQLANSEGIRTSKLFSKILTNDFQS
                     IAVKQQADRIEDLVNRLEKRIDDFEEDNNFSEKYYEDFSGLYFMMLWLLMKNGASKDEV
                     RMIQAKGYSYADDNYKRVK"
                     /name="hypothetical protein"
     gene            complement(3231..3485)
                     /locus_tag="MTS78_RS19985"
     CDS             complement(3231..3485)
                     /locus_tag="MTS78_RS19985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001014303.1"
                     /translation="MNGQKIDFLRFSKQGNKYFFPCKTKHRPYVTLANFPIQDPAHAGI
                     FLSHTTHLTMTPLKALQDKDFSDFLGSQKRVQKNGLFLE"
                     /name="hypothetical protein"
     gene            4169..5119
                     /gene="repM"
                     /locus_tag="MTS78_RS19935"
                     /old_locus_tag="MTS78_19935"
     CDS             4169..5119
                     /gene="repM"
                     /locus_tag="MTS78_RS19935"
                     /old_locus_tag="MTS78_19935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001205343.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHADSYINQFGVHRNTAYQALKDACKDLFARQFSYQEKKANGNIRNVMSRWVSQIAY
                     NDNEATVDLIFAPAVVPFITRLEEQFTKYELQQVSSLSSAYAIRLYELLIQWRSTGKTP
                     TIELQEFRKKLGVLDNEYLRMAHLKERVLELSIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDSPSIERDPNTLELFSKMTDAQRHMFANKLSELPEMGRYSQGTESYQQFAV
                     RIAEMLQDPAQFKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            5112..5687
                     /locus_tag="MTS78_RS19940"
                     /old_locus_tag="MTS78_19940"
     CDS             5112..5687
                     /locus_tag="MTS78_RS19940"
                     /old_locus_tag="MTS78_19940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_001096616.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTSQKIESSKQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATAAPHQDATACDTKMPQHATTQQDTDNKKNNELNIAENVAVPQQETTAYHTQTLQH
                     ATLQSVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            5707..5850
                     /locus_tag="MTS78_RS19945"
                     /old_locus_tag="MTS78_19945"
     CDS             5707..5850
                     /locus_tag="MTS78_RS19945"
                     /old_locus_tag="MTS78_19945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            complement(6039..6557)
                     /locus_tag="MTS78_RS19950"
                     /old_locus_tag="MTS78_19950"
     CDS             complement(6039..6557)
                     /locus_tag="MTS78_RS19950"
                     /old_locus_tag="MTS78_19950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447193.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_000447193.1"
                     /translation="METFAEPVFSNDLLAKAENGDTSAQLELAEIYLYGHGVDSDENQA
                     EVWALKSAENGNVAAMFWLADGYVTYARLMEDDDKNDSLEHFQKAFKWFQKASENGHSE
                     SMVELADLYTRADSGIEVNINKAFELREKAAKLGNKKAMRSLSVMYRDGIGIPKNTDLA
                     QSWWDKSEN"
                     /name="tetratricopeptide repeat protein"
     gene            6930..7217
                     /locus_tag="MTS78_RS19955"
                     /old_locus_tag="MTS78_19955"
     CDS             6930..7217
                     /locus_tag="MTS78_RS19955"
                     /old_locus_tag="MTS78_19955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438826.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            7204..7518
                     /locus_tag="MTS78_RS19960"
                     /old_locus_tag="MTS78_19960"
     CDS             7204..7518
                     /locus_tag="MTS78_RS19960"
                     /old_locus_tag="MTS78_19960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     Typing          4170..5119
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggtattgaca aaattcgtac ctcattgagt tatatagatt attttcacaa tgaactcgag
       61 ggagataaga ttactaactt ttttaaaaat acaggaaaag taggccgcat agagttatca
      121 catcagccat taggggaatt aaccggtatc ttggggctac aatatttgga acaggataat
      181 agtgctttat caccagtcca ttctcaagaa ggccatacca cctatctaga taatcagcag
      241 ctattaaatc gaaatgtcac taaaaacttt agtgtatttg gcctagaaaa atataactgg
      301 aacgacttta ctttcgaact aggtgctcgt atagaaaagc agaaagttag tatggattat
      361 gatatagaaa aaattaaaga ttcaatgaaa ccttggccta ataaatataa cagtccttat
      421 gtagaaaaaa ataataaaat cagggctcaa aatttaaaat ctattttaga agctgttcaa
      481 cctaacaaag aaacagcttt ttcttatgca ggtacagtcc actggagatt tgctccaaat
      541 tatatcttat cattaacagg tacacatcaa gaaagattac ctaatgcaca agaaatgtat
      601 actcatggta tgcatctcgc tactaattct tttgaaatag gtaaccgctt tctaagaaaa
      661 gagaaatcca ataatttaga aatcagtctg gcctataagg atgatttact tgattatcaa
      721 atcagtactt actattatga ttttgataac tatatatatt tacagacact caatgaagtt
      781 ttaggaacta caaaagttag agatcagcat acactacgta ttaatcatta tagccagtct
      841 gctgctaatt tttatggatt agagggcaac atagggtatc aatttaattc tgtttatcac
      901 ggctctcttt ttggagatta tgtgaaaggc cgtttaacaa atttacctga tgctgtaatt
      961 gcatacgata tctggaatag agaaccgact ttagctcccc aaaaggatcg ttacactcct
     1021 agattacctc ctgctcgttt gggtacacga ctaaaagctg attttgatga atcgcttaaa
     1081 ggtgaaatag aatattatcg agtctttaaa caagataata tttctaaatt tgagcaggtt
     1141 acatcaggtt ataatatgct taatatgact ttagcatata aaaataagct atctcacaca
     1201 gaatatgatt tattctttaa agcgaataat ttacttgatc agaaagtcta tgcccatgaa
     1261 acatttttgc catatattcc ccaaattgga agaaatttta gtttaggctt aaatcttaat
     1321 ttctagataa gatactgtaa tcttctaaga aaatttctta gaagattttt cttataaaat
     1381 gattagatta ataattaagg taagcgtccg ctgaatccca tacctatttt ttaattcgat
     1441 ttaggtttcc agcagtgtgg cagtaactct tcaatctggg tcactttatg tgtcggcagc
     1501 cttttcagca ctgaatagcc accgattttg tagacacctt ttagctagat aaaaagcaat
     1561 ttttttgatc tttaaagaat gaggatataa gctgtttaaa acttaattct aattggttaa
     1621 taggtgaaaa atgaaattag cagaagctct cttacttcga agtgatcaac aaaaaaaaat
     1681 tatttcacta aaacaacgta tcaatgcaaa tgtattggta caagatggtg atcaaccatc
     1741 tgaagatcca aatgaactac taaaacaagt attttcttta attcaagaat ttcaaaaatt
     1801 aagttatgca attcatgaaa ctaatgcttt aacaaaatta aatgatggcc gttctctact
     1861 tgctttattg actttgcgtg atgaatttgt agaacagcac aaaaccctaa ctgcagcaat
     1921 aagtaataca tctcgtgaat ctgatcgtta tagtacgcgg gaaataaagt ggcataaagt
     1981 tattccagtt tcatcattac aaaaacaagc tgatgatatt agtcttaaat tacgtgattt
     2041 aaacgtgtta attcagtcga ctaattggaa aattgatttg attgaagcct aatagtcctt
     2101 tagatataat caagttgaac atactgggcg agtacaagat cccttacttc cacaacatag
     2161 gattgtaaat aaagtgtagt ggcttgcaaa gcaagtggat aagcttaaaa ttttaagtac
     2221 aaatcaatcc atcacttggc ataactcaaa tctttgtgca aacaaagtta cacgttactt
     2281 ataactacca tgtactgaca gtatgttcac ctcaattact aaaaataaag aaatgaacaa
     2341 gcatcagctc aactacattt ctatttttaa ttcagttcgg catttaacat aaaatctctt
     2401 atacgaagcc caggttaatt ctgggctttt tgttcaatct catcattggc cgagaacata
     2461 cttggattcg taagcctgat attttcctga caacttccac acagcatcaa tcctgcaaaa
     2521 agttgtacat tttgtctatt ttcacaaagc gtacatggct ttgaaagctg aataatatta
     2581 tctgacacta ctttaccctc ttatagttat catctgcata tgaataacct ttagcttgta
     2641 tcattctaac ctcatcttta gaagcgccat ttttcattaa gagccacaac atcatgaaat
     2701 atagtccaga aaagtcttca tagtattttt cagaaaaatt gttatcttcc tcaaaatcat
     2761 ctattcgctt ttctaatcga ttcaccagat cctcaatacg atcggcttgc tgtttaacag
     2821 caatagattg aaaatcattg gtcaaaattt tactgaataa tttactggtt ctgattcctt
     2881 ctgaattagc caattgatcg tagaattctg ctacttgtgg atctaacctg actgcataag
     2941 ttttgacttt ttcgcccata aattacacct cttattaaaa ttagattttg tacaaaatta
     3001 aatatatata attcaactaa ttaaataaaa aataataatt agattacgta caaacttatt
     3061 tgtacagaat taatttattc taaaacaaaa acttatttaa atataatgct ttttttatgt
     3121 acaatcatga gtacatattt tttcttaaat ttatcaatat cttatctaaa aaaagtgttg
     3181 ttttgatgta caaccatgag cacagcataa aaaatagata attcaatcac ttattctaaa
     3241 aaaagaccat ttttctgtac acgtttctga gaccctaaaa aatcgctgaa atccttatcc
     3301 tgtaaggctt tgaggggtgt catcgttaga tgcgtggtgt gtgacaaaaa aatcccggca
     3361 tgtgccggat cttggattgg aaaattggct aaagtgacgt agggccggtg cttggttttg
     3421 catggaaaaa agtatttatt tccttgttta gaaaaacgta aaaaatcaat tttttgtcca
     3481 ttcatccagg ttaaaaattt caacccaaaa ctttaattat gaaaagcttc atagaacgcg
     3541 ttcaaatgcg atttaagagc ctttacctag aaaacataga tctcatagtg aaaaacacaa
     3601 aacagctcaa aaaacgcaaa tgagagcgaa ataaagaggt attttgactt tagatagatg
     3661 catagagcga gtgtctacga gcgaactatc aaaatttgcg cctagaccct ctgaaaaaca
     3721 tttttttgtc ctctttagcc taagaaagcg taagtttcgt gcagaaattt gctcctggat
     3781 cgagcgtagc gagtaaaaaa gttttatgag cgaagcgaat tccaagtttc ttttgctttt
     3841 ttttaaagtc acgccagtat taaccaaaaa attgccccaa cgaactgagc gaaagcgaag
     3901 ttcaatagag tttgagcgaa gcgaaaacca agggcaattt ttcattccct gggcttttaa
     3961 ttattttaaa gtttttaaat acttttaaat ggcttgaaag ccttatgaat aaagggtttt
     4021 agctcttata tgtccacgtt taccttgcaa tatgtccacg tttaccttgc aatatgtcca
     4081 cgtttacctt gcaatatgtc cacgtttacc ttgcattagt acacaaataa tattaacgtg
     4141 tacttataca caataaaaaa tagtggctat gagagattta gttgtaaagg acaatgcctt
     4201 aatcaacgca agctataact tagacttagt agaacaacgt ttaattttat tggctattgt
     4261 tgaagcaagg gaaagtggga aagggattaa tgcaaatgat ccattagaag ttcatgcaga
     4321 tagttatatc aatcaatttg gtgtacaccg taatacagct tatcaagcct taaaagatgc
     4381 ttgtaaggat ttattcgcgc gtcaatttag ctatcaagag aaaaaagcta atgggaatat
     4441 ccgaaatgtt atgagtcgtt gggtatctca aattgcttat aacgacaatg aagcaactgt
     4501 agacttaata tttgcacctg ctgttgttcc cttcataacc cgactggaag aacaatttac
     4561 taaatatgaa ttacagcaag ttagtagtct tagtagcgct tatgccattc gcttatatga
     4621 gcttctaatt cagtggcgaa gcactggtaa aaccccaacc atagaactac aagaatttag
     4681 aaagaagtta ggcgttcttg ataatgaata tttacggatg gctcatttaa aagagcgtgt
     4741 tttagagctt tcaattaaac aaataaatga gcatacggat ataactgtaa aatatgaaca
     4801 gcataaaaga ggacgttcta tttcaggatt ttcttttacc tttaaacaga agaagaagga
     4861 tagcccatca atagaaagag atccgaacac tttggagctt ttttcaaaga tgaccgatgc
     4921 tcaacggcat atgtttgcaa ataaactttc agaactccct gaaatgggtc gctattcaca
     4981 aggaacagaa agctaccaac agtttgctgt acgtattgct gagatgctac aagatcccgc
     5041 tcaattcaaa gagctatacc catacctaaa aaaagtggga tacatgccat caaataaaaa
     5101 ggacaccgta aatggctaag ttatcactaa gtgaagtatc taaaaaattt catgtggata
     5161 gatcaaccat ttacagagct gtacgtaatg gacgtttatc acgctccagt gatggacaat
     5221 tcgatctagc agaggtcata cgatgctttg gagaacctga gcaaacatct caaaaaattg
     5281 aatcatctaa gcaagaaggt gatgaatcta caaaaaaact tattgcccat ttagaaaatg
     5341 aagtcaaaaa ataccaagaa cgtgaagaac ggttaatgca acaaattgac cgtatgcaaa
     5401 cactcattga gctgaaaagt gttgcacctg ccacagcagc accacaccaa gatgctacgg
     5461 catgcgacac caagatgcca cagcatgcga caacacaaca agacactgat aacaaaaaga
     5521 ataatgaatt aaatattgca gaaaatgtag cagtgccaca gcaagaaact acggcatacc
     5581 acacccaaac gctacagcat gccacgttgc aaagtgtggc agtgccacaa cacaaaaaac
     5641 gtggtttatt tggccgtgtg ctgaatgccg tctttgataa tgactgagca ataagggtga
     5701 aagatcatgc cgaaactgaa agacattgcc ctgggaatta ttgtagctcc gctgctgatc
     5761 ccgatcatgc tgattgcatc gtaccaggat aaaaaggcgc tgaagaaaga gctggatgaa
     5821 tgccaaaaag aaaaagacca ggcatcctga ttattcaatt gagctaatca aaagtgtgga
     5881 acattgatga aataatagcg tggaacgtga aaagtcataa aaaaagcccg aacagggatg
     5941 ctcgggctat aaactagaaa ctacagcgtt gaattataag gataattata cagcatttcg
     6001 tataaggtgt attatgttaa ttttaggaaa tattaaaact aattttcaga cttatcccac
     6061 caactttgag ctaaatcagt atttttagga atacctatac catcacgata cataacggaa
     6121 agacttcgca ttgctttctt attacctaac tttgcagcct tttcacgaag ttcaaaagct
     6181 ttattaatat taacttctat tccgctatct gcacgagtat atagatcagc taactcaacc
     6241 attgattcag aatggccatt ttctgaggct ttctgaaacc acttaaaagc tttttggaaa
     6301 tgttctaaag aatcgttttt gtcatcatct tccattaatc tagcataagt aacatatcca
     6361 tcagctaacc aaaacattgc tgctacattt ccattttctg ctgatttaag agcccaaact
     6421 tcagcttgat tttcatctga atcaacacca tgaccatata gataaatctc ggctaattct
     6481 aactgagcag aagtatctcc attttctgct ttcgctaata aatcattaga aaaaacaggt
     6541 tctgcaaatg tttccattga aaggcaaact cctaaagcca taaaaatatt tgttaattta
     6601 ttcaattttt aacctttaaa ctaaactgtt aggcttgtat atttttatat catacctaaa
     6661 agaaaactct aaaattagag tatgtcttat ctctatttaa cataatggcg attatacgaa
     6721 gtacacttgt atattaatat aaatatcaac tacttaataa aaatagaaaa atcccaaaat
     6781 gcccaaaaag ccgactttga aacaagtcgg cttttttatg agcagttttg atacttcttg
     6841 ccaataattt tgactaaaaa ttacacgaat ctggttgagt gatacaattt tgtgatacat
     6901 tattgtatat acaaatttga atgtttttta tggaacagta ttttgaatgg gatgaggcta
     6961 agaatcgaaa gaatcagaaa aagcatgata tctctttcga aactgcaagc cttgtttttg
     7021 aagatccatt acggatctca atccaagaca gacataccaa tggtgaagaa cgttggcaaa
     7081 ccattggacg agttaaaggc gtactaatgt tattggtggc tcacaccatt tttgatgaag
     7141 atgactgtga aatcatccga atcattagtg caaggcaagt cactaaagcg gagcgaaaca
     7201 aatatgagca tggttagata ctcacacaaa gagctgaatg agaaatttgg tgagaagcaa
     7261 gatgctgaaa ttcaacgctt gcttgctaaa ggcacggttc ctgatgatca gctagatcta
     7321 tcagatattc ctgaaattac agactggagc aatgctgtac gccaaaatca attttaccgc
     7381 ccagtaaagc aacaaacttc aattcggtta gatgctgatg tacttgcttg gttcaaagca
     7441 caaggcaaag gttatcaaac acgtatgaat gaaattttac gagatgctat gctaaaagaa
     7501 ttaaaaaatc atcaataaga atgggagctt aggctcccat ttttgacttc atttttaggg
     7561 gggattacca ttcaaaaatt taaatagtag attttataaa tttttaagtg ttatattata
     7621 atataacatt aaatttggtt gttttatgct aaataaaagt aaactatttt tagctttaat
     7681 tactttaggg gcctcgaaaa ttttattggc agctgaaggg cctgttacta ccttaaatac
     7741 aatagtatta actgcacaat ctgatgagtt agggtctgag ctgttaggca aaagtctaaa
     7801 tgttagtaat cagtttattg atactagtaa acttaaacag cgttcgacaa ctctagggga
     7861 tgccttgggt acggaattag gaattcattc taatcaatat ggaggtggag catctactcc
     7921 tattattcgt gggcaggaag gaaaacgtat taaagtttta cagaataatg ctgacgtgtt
     7981 ggatatgtct aatatgtccc cagatcacgc tgtcacagtt gaaccaagtt tagctaagtc
     8041 tattgaaatt atacgtggtg caagtaccct tttgtatagt tcaaatagtg cggcaggggt
     8101 agtcaatgtc attgactata aaattccaac ccaaatgcct caagacggat tagagggtaa
     8161 tactacatta cgttttaata ctggcagtaa tgagaagttg accacagcag gtgtaacagt
     8221 tggcttaagc ccacatgtag ctttacgggc agaagggtta tatcgtaatg ctggtaacta
     8281 taaaactcct cattaccagt ctagctctta taattcttta gaagatcttg aaaaccaaaa
     8341 cagtatttat aaaaatttaa aatatttacc agaaagctgg gcagagtcga gagtaggtac
     8401 gcttggatta tcctggatag acgataatac ttatttagga gtttcttata cacatcgtca
     8461 tgatgaatat ggtttaccag cccatagcca tttatatgaa ggttgtggtg cctctgcaat
     8521 cggtatcgat tcacgaatta gtggattaaa aaattatctt ttatattatc ctcaattgat
     8581 ggatgagcaa gatattaatt acataaatcc acgtcctgat tgtcatcagc ataatcatat
     8641 tcatgaaact aatttctctc ataatgctcc ttatattgat ttaaatactc gacgttatga
     8701 tgtgcgtgga gaattcactc aac
//
