LOCUS       NZ_CP121590             8730 bp    DNA     circular CON 17-MAY-2024
DEFINITION  Acinetobacter baumannii strain MAB9 plasmid pI_MAB9, complete
            sequence.
ACCESSION   NZ_CP121590
VERSION     NZ_CP121590.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27010256
            Assembly: GCF_029714165.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 8730)
  AUTHORS   Alghorbi,M.F., Bawazeer,R., Okdah,L., Alswaji,A., Alzayer,M.A.,
            Alrashidi,E., Alshahrani,A.S., Al Johani,S.M., NGHA Surveillance
            Group,N.S.G., Balkhy,H. and Doumith,M.
  TITLE     Antimicrobial resistance and genomic characterisation of
            Acinetobacter baumannii clinical isolates from a nation
            representative surveillance in Saudi Arabia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8730)
  AUTHORS   Alghorbi,M.F., Bawazeer,R., Okdah,L., Alswaji,A., Alzayer,M.A.,
            Alrashidi,E., Alshahrani,A.S., Al Johani,S.M., NGHA Surveillance
            Group,N.S.G., Balkhy,H. and Doumith,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2023) Infectious Diseases Research, King Abdullah
            International Medical Research Center (KAIMRC), KAIMRC, P.O Box
            22490, Riyadh 11426, Saudi Arabia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: EToKi v. 1.2
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 139x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029714165.1-RS_2024_05_17
            Annotation Date                   :: 05/17/2024 04:53:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,012
            CDSs (total)                      :: 3,916
            Genes (coding)                    :: 3,831
            CDSs (with protein)               :: 3,831
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 21 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 5 of 85
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP121590.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8730
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="MAB9"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pI_MAB9"
                     /geo_loc_name="Saudi Arabia:Medina"
                     /lat_lon="24.5247 N 39.5692 E"
                     /collection_date="2019"
                     /collected_by="KAIMRC AMR WGS_Based Surveillance Program"
     gene            join(8633..8730,1..2314)
                     /gene="znuD2"
                     /locus_tag="MTS46_RS20050"
                     /old_locus_tag="MTS46_20050"
     CDS             join(8633..8730,1..2314)
                     /gene="znuD2"
                     /locus_tag="MTS46_RS20050"
                     /old_locus_tag="MTS46_20050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            2618..3079
                     /locus_tag="MTS46_RS20055"
                     /old_locus_tag="MTS46_20055"
     CDS             2618..3079
                     /locus_tag="MTS46_RS20055"
                     /old_locus_tag="MTS46_20055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(3402..3575)
                     /locus_tag="MTS46_RS20060"
                     /old_locus_tag="MTS46_20060"
     CDS             complement(3402..3575)
                     /locus_tag="MTS46_RS20060"
                     /old_locus_tag="MTS46_20060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001282484.1"
                     /translation="MSDNIIQLSKPCTLCENRQNVQLFAGLMLCGSCQENIRLTNPSMF
                     SANDEIEQKAQN"
                     /name="hypothetical protein"
     gene            complement(3575..3946)
                     /locus_tag="MTS46_RS20065"
                     /old_locus_tag="MTS46_20065"
     CDS             complement(3575..3946)
                     /locus_tag="MTS46_RS20065"
                     /old_locus_tag="MTS46_20065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000504218.1"
                     /translation="MGEKVKTYAVRLDPQVAEFYDQLANSEGIRTSKLFSKILTNDFQS
                     IAVKQQADRIEDLVNRLEKRIDDFEEDNNFSEKYYEDFSGLYFMMLWLLMKNGASKDEV
                     RMIQAKGYSYADDNYKRVK"
                     /name="hypothetical protein"
     gene            complement(4218..4472)
                     /locus_tag="MTS46_RS20130"
     CDS             complement(4218..4472)
                     /locus_tag="MTS46_RS20130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001014303.1"
                     /translation="MNGQKIDFLRFSKQGNKYFFPCKTKHRPYVTLANFPIQDPAHAGI
                     FLSHTTHLTMTPLKALQDKDFSDFLGSQKRVQKNGLFLE"
                     /name="hypothetical protein"
     gene            5156..6106
                     /gene="repM"
                     /locus_tag="MTS46_RS20070"
                     /old_locus_tag="MTS46_20070"
     CDS             5156..6106
                     /gene="repM"
                     /locus_tag="MTS46_RS20070"
                     /old_locus_tag="MTS46_20070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001205343.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHADSYINQFGVHRNTAYQALKDACKDLFARQFSYQEKKANGNIRNVMSRWVSQIAY
                     NDNEATVDLIFAPAVVPFITRLEEQFTKYELQQVSSLSSAYAIRLYELLIQWRSTGKTP
                     TIELQEFRKKLGVLDNEYLRMAHLKERVLELSIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDSPSIERDPNTLELFSKMTDAQRHMFANKLSELPEMGRYSQGTESYQQFAV
                     RIAEMLQDPAQFKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            6099..6674
                     /locus_tag="MTS46_RS20075"
                     /old_locus_tag="MTS46_20075"
     CDS             6099..6674
                     /locus_tag="MTS46_RS20075"
                     /old_locus_tag="MTS46_20075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_001096616.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTSQKIESSKQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATAAPHQDATACDTKMPQHATTQQDTDNKKNNELNIAENVAVPQQETTAYHTQTLQH
                     ATLQSVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            6694..6837
                     /locus_tag="MTS46_RS20080"
                     /old_locus_tag="MTS46_20080"
     CDS             6694..6837
                     /locus_tag="MTS46_RS20080"
                     /old_locus_tag="MTS46_20080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            complement(7026..7544)
                     /locus_tag="MTS46_RS20085"
                     /old_locus_tag="MTS46_20085"
     CDS             complement(7026..7544)
                     /locus_tag="MTS46_RS20085"
                     /old_locus_tag="MTS46_20085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447193.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_000447193.1"
                     /translation="METFAEPVFSNDLLAKAENGDTSAQLELAEIYLYGHGVDSDENQA
                     EVWALKSAENGNVAAMFWLADGYVTYARLMEDDDKNDSLEHFQKAFKWFQKASENGHSE
                     SMVELADLYTRADSGIEVNINKAFELREKAAKLGNKKAMRSLSVMYRDGIGIPKNTDLA
                     QSWWDKSEN"
                     /name="tetratricopeptide repeat protein"
     gene            7917..8204
                     /locus_tag="MTS46_RS20090"
                     /old_locus_tag="MTS46_20090"
     CDS             7917..8204
                     /locus_tag="MTS46_RS20090"
                     /old_locus_tag="MTS46_20090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438826.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            8191..8505
                     /locus_tag="MTS46_RS20095"
                     /old_locus_tag="MTS46_20095"
     CDS             8191..8505
                     /locus_tag="MTS46_RS20095"
                     /old_locus_tag="MTS46_20095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     Typing          5157..6106
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agtattaact gcacaatctg atgagttagg gtctgagctg ttaggcaaaa gtctaaatgt
       61 tagtaatcag tttattgata ctagtaaact taaacagcgt tcgacaactc taggggatgc
      121 cttgggtacg gaattaggaa ttcattctaa tcaatatgga ggtggagcat ctactcctat
      181 tattcgtggg caggaaggaa aacgtattaa agttttacag aataatgctg acgtgttgga
      241 tatgtctaat atgtccccag atcacgctgt cacagttgaa ccaagtttag ctaagtctat
      301 tgaaattata cgtggtgcaa gtaccctttt gtatagttca aatagtgcgg caggggtagt
      361 caatgtcatt gactataaaa ttccaaccca aatgcctcaa gacggattag agggtaatac
      421 tacattacgt tttaatactg gcagtaatga gaagttgacc acagcaggtg taacagttgg
      481 cttaagccca catgtagctt tacgggcaga agggttatat cgtaatgctg gtaactataa
      541 aactcctcat taccagtcta gctcttataa ttctttagaa gatcttgaaa accaaaacag
      601 tatttataaa aatttaaaat atttaccaga aagctgggca gagtcgagag taggtacgct
      661 tggattatcc tggatagacg ataatactta tttaggagtt tcttatacac atcgtcatga
      721 tgaatatggt ttaccagccc atagccattt atatgaaggt tgtggtgcct ctgcaatcgg
      781 tatcgattca cgaattagtg gattaaaaaa ttatctttta tattatcctc aattgatgga
      841 tgagcaagat attaattaca taaatccacg tcctgattgt catcagcata atcatattca
      901 tgaaactaat ttctctcata atgctcctta tattgattta aatactcgac gttatgatgt
      961 gcgtggagaa ttcactcaac catttaccgg tattgacaaa attcgtacct cattgagtta
     1021 tatagattat tttcacaatg aactcgaggg agataagatt actaactttt ttaaaaatac
     1081 aggaaaagta ggccgcatag agttatcaca tcagccatta ggggaattaa ccggtatctt
     1141 ggggctacaa tatttggaac aggataatag tgctttatca ccagtccatt ctcaagaagg
     1201 ccataccacc tatctagata atcagcagct attaaatcga aatgtcacta aaaactttag
     1261 tgtatttggc ctagaaaaat ataactggaa cgactttact ttcgaactag gtgctcgtat
     1321 agaaaagcag aaagttagta tggattatga tatagaaaaa attaaagatt caatgaaacc
     1381 ttggcctaat aaatataaca gtccttatgt agaaaaaaat aataaaatca gggctcaaaa
     1441 tttaaaatct attttagaag ctgttcaacc taacaaagaa acagcttttt cttatgcagg
     1501 tacagtccac tggagatttg ctccaaatta tatcttatca ttaacaggta cacatcaaga
     1561 aagattacct aatgcacaag aaatgtatac tcatggtatg catctcgcta ctaattcttt
     1621 tgaaataggt aaccgctttc taagaaaaga gaaatccaat aatttagaaa tcagtctggc
     1681 ctataaggat gatttacttg attatcaaat cagtacttac tattatgatt ttgataacta
     1741 tatatattta cagacactca atgaagtttt aggaactaca aaagttagag atcagcatac
     1801 actacgtatt aatcattata gccagtctgc tgctaatttt tatggattag agggcaacat
     1861 agggtatcaa tttaattctg tttatcacgg ctctcttttt ggagattatg tgaaaggccg
     1921 tttaacaaat ttacctgatg ctgtaattgc atacgatatc tggaatagag aaccgacttt
     1981 agctccccaa aaggatcgtt acactcctag attacctcct gctcgtttgg gtacacgact
     2041 aaaagctgat tttgatgaat cgcttaaagg tgaaatagaa tattatcgag tctttaaaca
     2101 agataatatt tctaaatttg agcaggttac atcaggttat aatatgctta atatgacttt
     2161 agcatataaa aataagctat ctcacacaga atatgattta ttctttaaag cgaataattt
     2221 acttgatcag aaagtctatg cccatgaaac atttttgcca tatattcccc aaattggaag
     2281 aaattttagt ttaggcttaa atcttaattt ctagataaga tactgtaatc ttctaagaaa
     2341 atttcttaga agatttttct tataaaatga ttagattaat aattaaggta agcgtccgct
     2401 gaatcccata cctatttttt aattcgattt aggtttccag cagtgtggca gtaactcttc
     2461 aatctgggtc actttatgtg tcggcagcct tttcagcact gaatagccac cgattttgta
     2521 gacacctttt agctagataa aaagcaattt ttttgatctt taaagaatga ggatataagc
     2581 tgttaaaact taattctaat tggttaatag gtgaaaaatg aaattagcag aagctctctt
     2641 acttcgaagt gatcaacaaa aaaaaattat ttcactaaaa caacgtatca atgcaaatgt
     2701 attggtacaa gatggtgatc aaccatctga agatccaaat gaactactaa aacaagtatt
     2761 ttctttaatt caagaatttc aaaaattaag ttatgcaatt catgaaacta atgctttaac
     2821 aaaattaaat gatggccgtt ctctacttgc tttattgact ttgcgtgatg aatttgtaga
     2881 acagcacaaa accctaactg cagcaataag taatacatct cgtgaatctg atcgttatag
     2941 tacgcgggaa ataaagtggc ataaagttat tccagtttca tcattacaaa aacaagctga
     3001 tgatattagt cttaaattac gtgatttaaa cgtgttaatt cagtcgacta attggaaaat
     3061 tgatttgatt gaagcctaat agtcctttag atataatcaa gttgaacata ctgggcgagt
     3121 acaagatccc ttacttccac aacataggat tgtaaataaa gtgtagtggc ttgcaaagca
     3181 agtggataag cttaaaattt taagtacaaa tcaatccatc acttggcata actcaaatct
     3241 ttgtgcaaac aaagttacac gttacttata actaccatgt actgacagta tgttcacctc
     3301 aattactaaa aataaagaaa tgaacaagca tcagctcaac tacatttcta tttttaattc
     3361 agttcggcat ttaacataaa atctcttata cgaagcccag gttaattctg ggctttttgt
     3421 tcaatctcat cattggccga gaacatactt ggattcgtaa gcctgatatt ttcctgacaa
     3481 cttccacaca gcatcaatcc tgcaaaaagt tgtacatttt gtctattttc acaaagcgta
     3541 catggctttg aaagctgaat aatattatct gacactactt taccctctta tagttatcat
     3601 ctgcatatga ataaccttta gcttgtatca ttctaacctc atctttagaa gcgccatttt
     3661 tcattaagag ccacaacatc atgaaatata gtccagaaaa gtcttcatag tatttttcag
     3721 aaaaattgtt atcttcctca aaatcatcta ttcgcttttc taatcgattc accagatcct
     3781 caatacgatc ggcttgctgt ttaacagcaa tagattgaaa atcattggtc aaaattttac
     3841 tgaataattt actggttctg attccttctg aattagccaa ttgatcgtag aattctgcta
     3901 cttgtggatc taacctgact gcataagttt tgactttttc gcccataaat tacacctctt
     3961 attaaaatta gattttgtac aaaattaaat atatataatt caactaatta aataaaaaat
     4021 aataattaga ttacgtacaa acttatttgt acagaattaa tttattctaa aacaaaaact
     4081 tatttaaata taatgctttt tttatgtaca atcatgagta catatttttt cttaaattta
     4141 tcaatatctt atctaaaaaa agtgttgttt tgatgtacaa ccatgagcac agcataaaaa
     4201 atagataatt caatcactta ttctaaaaaa agaccatttt tctgtacacg tttctgagac
     4261 cctaaaaaat cgctgaaatc cttatcctgt aaggctttga ggggtgtcat cgttagatgc
     4321 gtggtgtgtg acaaaaaaat cccggcatgt gccggatctt ggattggaaa attggctaaa
     4381 gtgacgtagg gccggtgctt ggttttgcat ggaaaaaagt atttatttcc ttgtttagaa
     4441 aaacgtaaaa aatcaatttt ttgtccattc atccaggtta aaaatttcaa cccaaaactt
     4501 taattatgaa aagcttcata gaacgcgttc aaatgcgatt taagagcctt tacctagaaa
     4561 acatagatct catagtgaaa aacacaaaac agctcaaaaa acgcaaatga gagcgaaata
     4621 aagaggtatt ttgactttag atagatgcat agagcgagtg tctacgagcg aactatcaaa
     4681 atttgcgcct agaccctctg aaaaacattt ttttgtcctc tttagcctaa gaaagcgtaa
     4741 gtttcgtgca gaaatttgct cctggatcga gcgtagcgag taaaaaagtt ttatgagcga
     4801 agcgaattcc aagtttcttt tgcttttttt taaagtcacg ccagtattaa ccaaaaaatt
     4861 gccccaacga actgagcgaa agcgaagttc aatagagttt gagcgaagcg aaaaccaagg
     4921 gcaatttttc attccctggg cttttaatta ttttaaagtt tttaaatact tttaaatggc
     4981 ttgaaagcct tatgaataaa gggttttagc tcttatatgt ccacgtttac cttgcaatat
     5041 gtccacgttt accttgcaat atgtccacgt ttaccttgca atatgtccac gtttaccttg
     5101 cattagtaca caaataatat taacgtgtac ttatacacaa taaaaaatag tggctatgag
     5161 agatttagtt gtaaaggaca atgccttaat caacgcaagc tataacttag acttagtaga
     5221 acaacgttta attttattgg ctattgttga agcaagggaa agtgggaaag ggattaatgc
     5281 aaatgatcca ttagaagttc atgcagatag ttatatcaat caatttggtg tacaccgtaa
     5341 tacagcttat caagccttaa aagatgcttg taaggattta ttcgcgcgtc aatttagcta
     5401 tcaagagaaa aaagctaatg ggaatatccg aaatgttatg agtcgttggg tatctcaaat
     5461 tgcttataac gacaatgaag caactgtaga cttaatattt gcacctgctg ttgttccctt
     5521 cataacccga ctggaagaac aatttactaa atatgaatta cagcaagtta gtagtcttag
     5581 tagcgcttat gccattcgct tatatgagct tctaattcag tggcgaagca ctggtaaaac
     5641 cccaaccata gaactacaag aatttagaaa gaagttaggc gttcttgata atgaatattt
     5701 acggatggct catttaaaag agcgtgtttt agagctttca attaaacaaa taaatgagca
     5761 tacggatata actgtaaaat atgaacagca taaaagagga cgttctattt caggattttc
     5821 ttttaccttt aaacagaaga agaaggatag cccatcaata gaaagagatc cgaacacttt
     5881 ggagcttttt tcaaagatga ccgatgctca acggcatatg tttgcaaata aactttcaga
     5941 actccctgaa atgggtcgct attcacaagg aacagaaagc taccaacagt ttgctgtacg
     6001 tattgctgag atgctacaag atcccgctca attcaaagag ctatacccat acctaaaaaa
     6061 agtgggatac atgccatcaa ataaaaagga caccgtaaat ggctaagtta tcactaagtg
     6121 aagtatctaa aaaatttcat gtggatagat caaccattta cagagctgta cgtaatggac
     6181 gtttatcacg ctccagtgat ggacaattcg atctagcaga ggtcatacga tgctttggag
     6241 aacctgagca aacatctcaa aaaattgaat catctaagca agaaggtgat gaatctacaa
     6301 aaaaacttat tgcccattta gaaaatgaag tcaaaaaata ccaagaacgt gaagaacggt
     6361 taatgcaaca aattgaccgt atgcaaacac tcattgagct gaaaagtgtt gcacctgcca
     6421 cagcagcacc acaccaagat gctacggcat gcgacaccaa gatgccacag catgcgacaa
     6481 cacaacaaga cactgataac aaaaagaata atgaattaaa tattgcagaa aatgtagcag
     6541 tgccacagca agaaactacg gcataccaca cccaaacgct acagcatgcc acgttgcaaa
     6601 gtgtggcagt gccacaacac aaaaaacgtg gtttatttgg ccgtgtgctg aatgccgtct
     6661 ttgataatga ctgagcaata agggtgaaag atcatgccga aactgaaaga cattgccctg
     6721 ggaattattg tagctccgct gctgatcccg atcatgctga ttgcatcgta ccaggataaa
     6781 aaggcgctga agaaagagct ggatgaatgc caaaaagaaa aagaccaggc atcctgatta
     6841 ttcaattgag ctaatcaaaa gtgtggaaca ttgatgaaat aatagcgtgg aacgtgaaaa
     6901 gtcataaaaa aagcccgaac agggatgctc gggctataaa ctagaaacta cagcgttgaa
     6961 ttataaggat aattatacag catttcgtat aaggtgtatt atgttaattt taggaaatat
     7021 taaaactaat tttcagactt atcccaccaa ctttgagcta aatcagtatt tttaggaata
     7081 cctataccat cacgatacat aacggaaaga cttcgcattg ctttcttatt acctaacttt
     7141 gcagcctttt cacgaagttc aaaagcttta ttaatattaa cttctattcc gctatctgca
     7201 cgagtatata gatcagctaa ctcaaccatt gattcagaat ggccattttc tgaggctttc
     7261 tgaaaccact taaaagcttt ttggaaatgt tctaaagaat cgtttttgtc atcatcttcc
     7321 attaatctag cataagtaac atatccatca gctaaccaaa acattgctgc tacatttcca
     7381 ttttctgctg atttaagagc ccaaacttca gcttgatttt catctgaatc aacaccatga
     7441 ccatatagat aaatctcggc taattctaac tgagcagaag tatctccatt ttctgctttc
     7501 gctaataaat cattagaaaa aacaggttct gcaaatgttt ccattgaaag gcaaactcct
     7561 aaagccataa aaatatttgt taatttattc aatttttaac ctttaaacta aactgttagg
     7621 cttgtatatt tttatatcat acctaaaaga aaactctaaa attagagtat gtcttatctc
     7681 tatttaacat aatggcgatt atacgaagta cacttgtata ttaatataaa tatcaactac
     7741 ttaataaaaa tagaaaaatc ccaaaatgcc caaaaagccg actttgaaac aagtcggctt
     7801 ttttatgagc agttttgata cttcttgcca ataattttga ctaaaaatta cacgaatctg
     7861 gttgagtgat acaattttgt gatacattat tgtatataca aatttgaatg ttttttatgg
     7921 aacagtattt tgaatgggat gaggctaaga atcgaaagaa tcagaaaaag catgatatct
     7981 ctttcgaaac tgcaagcctt gtttttgaag atccattacg gatctcaatc caagacagac
     8041 ataccaatgg tgaagaacgt tggcaaacca ttggacgagt taaaggcgta ctaatgttat
     8101 tggtggctca caccattttt gatgaagatg actgtgaaat catccgaatc attagtgcaa
     8161 ggcaagtcac taaagcggag cgaaacaaat atgagcatgg ttagatactc acacaaagag
     8221 ctgaatgaga aatttggtga gaagcaagat gctgaaattc aacgcttgct tgctaaaggc
     8281 acggttcctg atgatcagct agatctatca gatattcctg aaattacaga ctggagcaat
     8341 gctgtacgcc aaaatcaatt ttaccgccca gtaaagcaac aaacttcaat tcggttagat
     8401 gctgatgtac ttgcttggtt caaagcacaa ggcaaaggtt atcaaacacg tatgaatgaa
     8461 attttacgag atgctatgct aaaagaatta aaaaatcatc aataagaatg ggagcttagg
     8521 ctcccatttt tgacttcatt tttagggggg attaccattc aaaaatttaa atagtagatt
     8581 ttataaattt ttaagtgtta tattataata taacattaaa tttggttgtt ttatgctaaa
     8641 taaaagtaaa ctatttttag ctttaattac tttaggggcc tcgaaaattt tattggcagc
     8701 tgaagggcct gttactacct taaatacaat
//
