LOCUS       NZ_CP121587             8742 bp    DNA     circular CON 17-MAY-2024
DEFINITION  Acinetobacter baumannii strain RAB11 plasmid pI_RAB11, complete
            sequence.
ACCESSION   NZ_CP121587
VERSION     NZ_CP121587.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27010270
            Assembly: GCF_029713925.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 8742)
  AUTHORS   Alghorbi,M.F., Bawazeer,R., Okdah,L., Alswaji,A., Alzayer,M.A.,
            Alrashidi,E., Alshahrani,A.S., Al Johani,S.M., NGHA Surveillance
            Group,N.S.G., Balkhy,H. and Doumith,M.
  TITLE     Antimicrobial resistance and genomic characterisation of
            Acinetobacter baumannii clinical isolates from a nation
            representative surveillance in Saudi Arabia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8742)
  AUTHORS   Alghorbi,M.F., Bawazeer,R., Okdah,L., Alswaji,A., Alzayer,M.A.,
            Alrashidi,E., Alshahrani,A.S., Al Johani,S.M., NGHA Surveillance
            Group,N.S.G., Balkhy,H. and Doumith,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-APR-2023) Infectious Diseases Research, King Abdullah
            International Medical Research Center (KAIMRC), KAIMRC, P.O Box
            22490, Riyadh 11426, Saudi Arabia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: EToKi v. 1.2
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 172x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029713925.1-RS_2024_05_17
            Annotation Date                   :: 05/17/2024 04:54:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,880
            CDSs (total)                      :: 3,784
            Genes (coding)                    :: 3,681
            CDSs (with protein)               :: 3,681
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 48 of 103
            Pseudo Genes (incomplete)         :: 41 of 103
            Pseudo Genes (internal stop)      :: 22 of 103
            Pseudo Genes (multiple problems)  :: 8 of 103
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP121587.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8742
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="RAB11"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pI_RAB11"
                     /geo_loc_name="Saudi Arabia:Riyadh"
                     /lat_lon="24.7136 N 46.6753 E"
                     /collection_date="2018"
                     /collected_by="KAIMRC AMR WGS_Based Surveillance Program"
     gene            complement(join(8595..8742,1..118))
                     /locus_tag="MTS20_RS19500"
                     /pseudo=""
     CDS             complement(join(8595..8742,1..118))
                     /locus_tag="MTS20_RS19500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014301.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            802..1752
                     /gene="repM"
                     /locus_tag="MTS20_RS19390"
                     /old_locus_tag="MTS20_19390"
     CDS             802..1752
                     /gene="repM"
                     /locus_tag="MTS20_RS19390"
                     /old_locus_tag="MTS20_19390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001205343.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHADSYINQFGVHRNTAYQALKDACKDLFARQFSYQEKKANGNIRNVMSRWVSQIAY
                     NDNEATVDLIFAPAVVPFITRLEEQFTKYELQQVSSLSSAYAIRLYELLIQWRSTGKTP
                     TIELQEFRKKLGVLDNEYLRMAHLKERVLELSIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDSPSIERDPNTLELFSKMTDAQRHMFANKLSELPEMGRYSQGTESYQQFAV
                     RIAEMLQDPAQFKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            1745..2320
                     /locus_tag="MTS20_RS19395"
                     /old_locus_tag="MTS20_19395"
     CDS             1745..2320
                     /locus_tag="MTS20_RS19395"
                     /old_locus_tag="MTS20_19395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_001096616.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTSQKIESSKQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATAAPHQDATACDTKMPQHATTQQDTDNKKNNELNIAENVAVPQQETTAYHTQTLQH
                     ATLQSVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            2340..2483
                     /locus_tag="MTS20_RS19400"
                     /old_locus_tag="MTS20_19400"
     CDS             2340..2483
                     /locus_tag="MTS20_RS19400"
                     /old_locus_tag="MTS20_19400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            complement(2672..3190)
                     /locus_tag="MTS20_RS19405"
                     /old_locus_tag="MTS20_19405"
     CDS             complement(2672..3190)
                     /locus_tag="MTS20_RS19405"
                     /old_locus_tag="MTS20_19405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447193.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_000447193.1"
                     /translation="METFAEPVFSNDLLAKAENGDTSAQLELAEIYLYGHGVDSDENQA
                     EVWALKSAENGNVAAMFWLADGYVTYARLMEDDDKNDSLEHFQKAFKWFQKASENGHSE
                     SMVELADLYTRADSGIEVNINKAFELREKAAKLGNKKAMRSLSVMYRDGIGIPKNTDLA
                     QSWWDKSEN"
                     /name="tetratricopeptide repeat protein"
     gene            3563..3850
                     /locus_tag="MTS20_RS19410"
                     /old_locus_tag="MTS20_19410"
     CDS             3563..3850
                     /locus_tag="MTS20_RS19410"
                     /old_locus_tag="MTS20_19410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438826.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            3837..4151
                     /locus_tag="MTS20_RS19415"
                     /old_locus_tag="MTS20_19415"
     CDS             3837..4151
                     /locus_tag="MTS20_RS19415"
                     /old_locus_tag="MTS20_19415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            4279..6690
                     /gene="znuD2"
                     /locus_tag="MTS20_RS19420"
                     /old_locus_tag="MTS20_19420"
     CDS             4279..6690
                     /gene="znuD2"
                     /locus_tag="MTS20_RS19420"
                     /old_locus_tag="MTS20_19420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            6995..7456
                     /locus_tag="MTS20_RS19425"
                     /old_locus_tag="MTS20_19425"
     CDS             6995..7456
                     /locus_tag="MTS20_RS19425"
                     /old_locus_tag="MTS20_19425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(7779..7952)
                     /locus_tag="MTS20_RS19430"
                     /old_locus_tag="MTS20_19430"
     CDS             complement(7779..7952)
                     /locus_tag="MTS20_RS19430"
                     /old_locus_tag="MTS20_19430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001282484.1"
                     /translation="MSDNIIQLSKPCTLCENRQNVQLFAGLMLCGSCQENIRLTNPSMF
                     SANDEIEQKAQN"
                     /name="hypothetical protein"
     gene            complement(7952..8323)
                     /locus_tag="MTS20_RS19435"
                     /old_locus_tag="MTS20_19435"
     CDS             complement(7952..8323)
                     /locus_tag="MTS20_RS19435"
                     /old_locus_tag="MTS20_19435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000504218.1"
                     /translation="MGEKVKTYAVRLDPQVAEFYDQLANSEGIRTSKLFSKILTNDFQS
                     IAVKQQADRIEDLVNRLEKRIDDFEEDNNFSEKYYEDFSGLYFMMLWLLMKNGASKDEV
                     RMIQAKGYSYADDNYKRVK"
                     /name="hypothetical protein"
     Typing          803..1752
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gatcttggat tggaaaattg gctaaagtga cgtagggccg gtgcttggtt ttgcatggaa
       61 aaaagtattt atttccttgt ttagaaaaac gtaaaaaatc aattttttgt ccattcatcc
      121 aggttaaaaa tttcaaccca aaactttaat tatgaaaagc ttcatagaac gcgttcaaat
      181 gcgatttaag agcctttacc tagaaaacat agatctcata gtgaaaaaca caaaacagct
      241 caaaaaacgc aaatgagagc gaaataaaga ggtattttga ctttagatag atgcatagag
      301 cgagtgtcta cgagcgaact atcaaaattt gcgcctagac cctctgaaaa acattttttt
      361 gtcctcttta gcctaagaaa gcgtaagttt cgtgcagaaa tttgctcctg gatcgagcgt
      421 agcgagtaaa aaagttttat gagcgaagcg aattccaagt ttcttttgct tttttttaaa
      481 gtcacgccag tattaaccaa aaaattgccc caacgaactg agcgaaagcg aagttcaata
      541 gagtttgagc gaagcgaaaa ccaagggcaa tttttcattc cctgggcttt taattatttt
      601 aaagttttta aatactttta aatggcttga aagccttatg aataaagggt tttagctctt
      661 atatgtccac gtttaccttg caatatgtcc acgtttacct tgcaatatgt ccacgtttac
      721 cttgcaatat gtccacgttt accttgcatt agtacacaaa taatattaac gtgtacttat
      781 acacaataaa aaatagtggc tatgagagat ttagttgtaa aggacaatgc cttaatcaac
      841 gcaagctata acttagactt agtagaacaa cgtttaattt tattggctat tgttgaagca
      901 agggaaagtg ggaaagggat taatgcaaat gatccattag aagttcatgc agatagttat
      961 atcaatcaat ttggtgtaca ccgtaataca gcttatcaag ccttaaaaga tgcttgtaag
     1021 gatttattcg cgcgtcaatt tagctatcaa gagaaaaaag ctaatgggaa tatccgaaat
     1081 gttatgagtc gttgggtatc tcaaattgct tataacgaca atgaagcaac tgtagactta
     1141 atatttgcac ctgctgttgt tcccttcata acccgactgg aagaacaatt tactaaatat
     1201 gaattacagc aagttagtag tcttagtagc gcttatgcca ttcgcttata tgagcttcta
     1261 attcagtggc gaagcactgg taaaacccca accatagaac tacaagaatt tagaaagaag
     1321 ttaggcgttc ttgataatga atatttacgg atggctcatt taaaagagcg tgttttagag
     1381 ctttcaatta aacaaataaa tgagcatacg gatataactg taaaatatga acagcataaa
     1441 agaggacgtt ctatttcagg attttctttt acctttaaac agaagaagaa ggatagccca
     1501 tcaatagaaa gagatccgaa cactttggag cttttttcaa agatgaccga tgctcaacgg
     1561 catatgtttg caaataaact ttcagaactc cctgaaatgg gtcgctattc acaaggaaca
     1621 gaaagctacc aacagtttgc tgtacgtatt gctgagatgc tacaagatcc cgctcaattc
     1681 aaagagctat acccatacct aaaaaaagtg ggatacatgc catcaaataa aaaggacacc
     1741 gtaaatggct aagttatcac taagtgaagt atctaaaaaa tttcatgtgg atagatcaac
     1801 catttacaga gctgtacgta atggacgttt atcacgctcc agtgatggac aattcgatct
     1861 agcagaggtc atacgatgct ttggagaacc tgagcaaaca tctcaaaaaa ttgaatcatc
     1921 taagcaagaa ggtgatgaat ctacaaaaaa acttattgcc catttagaaa atgaagtcaa
     1981 aaaataccaa gaacgtgaag aacggttaat gcaacaaatt gaccgtatgc aaacactcat
     2041 tgagctgaaa agtgttgcac ctgccacagc agcaccacac caagatgcta cggcatgcga
     2101 caccaagatg ccacagcatg cgacaacaca acaagacact gataacaaaa agaataatga
     2161 attaaatatt gcagaaaatg tagcagtgcc acagcaagaa actacggcat accacaccca
     2221 aacgctacag catgccacgt tgcaaagtgt ggcagtgcca caacacaaaa aacgtggttt
     2281 atttggccgt gtgctgaatg ccgtctttga taatgactga gcaataaggg tgaaagatca
     2341 tgccgaaact gaaagacatt gccctgggaa ttattgtagc tccgctgctg atcccgatca
     2401 tgctgattgc atcgtaccag gataaaaagg cgctgaagaa agagctggat gaatgccaaa
     2461 aagaaaaaga ccaggcatcc tgattattca attgagctaa tcaaaagtgt ggaacattga
     2521 tgaaataata gcgtggaacg tgaaaagtca taaaaaaagc ccgaacaggg atgctcgggc
     2581 tataaactag aaactacagc gttgaattat aaggataatt atacagcatt tcgtataagg
     2641 tgtattatgt taattttagg aaatattaaa actaattttc agacttatcc caccaacttt
     2701 gagctaaatc agtattttta ggaataccta taccatcacg atacataacg gaaagacttc
     2761 gcattgcttt cttattacct aactttgcag ccttttcacg aagttcaaaa gctttattaa
     2821 tattaacttc tattccgcta tctgcacgag tatatagatc agctaactca accattgatt
     2881 cagaatggcc attttctgag gctttctgaa accacttaaa agctttttgg aaatgttcta
     2941 aagaatcgtt tttgtcatca tcttccatta atctagcata agtaacatat ccatcagcta
     3001 accaaaacat tgctgctaca tttccatttt ctgctgattt aagagcccaa acttcagctt
     3061 gattttcatc tgaatcaaca ccatgaccat atagataaat ctcggctaat tctaactgag
     3121 cagaagtatc tccattttct gctttcgcta ataaatcatt agaaaaaaca ggttctgcaa
     3181 atgtttccat tgaaaggcaa actcctaaag ccataaaaat atttgttaat ttattcaatt
     3241 tttaaccttt aaactaaact gttaggcttg tatattttta tatcatacct aaaagaaaac
     3301 tctaaaatta gagtatgtct tatctctatt taacataatg gcgattatac gaagtacact
     3361 tgtatattaa tataaatatc aactacttaa taaaaataga aaaatcccaa aatgcccaaa
     3421 aagccgactt tgaaacaagt cggctttttt atgagcagtt ttgatacttc ttgccaataa
     3481 ttttgactaa aaattacacg aatctggttg agtgatacaa ttttgtgata cattattgta
     3541 tatacaaatt tgaatgtttt ttatggaaca gtattttgaa tgggatgagg ctaagaatcg
     3601 aaagaatcag aaaaagcatg atatctcttt cgaaactgca agccttgttt ttgaagatcc
     3661 attacggatc tcaatccaag acagacatac caatggtgaa gaacgttggc aaaccattgg
     3721 acgagttaaa ggcgtactaa tgttattggt ggctcacacc atttttgatg aagatgactg
     3781 tgaaatcatc cgaatcatta gtgcaaggca agtcactaaa gcggagcgaa acaaatatga
     3841 gcatggttag atactcacac aaagagctga atgagaaatt tggtgagaag caagatgctg
     3901 aaattcaacg cttgcttgct aaaggcacgg ttcctgatga tcagctagat ctatcagata
     3961 ttcctgaaat tacagactgg agcaatgctg tacgccaaaa tcaattttac cgcccagtaa
     4021 agcaacaaac ttcaattcgg ttagatgctg atgtacttgc ttggttcaaa gcacaaggca
     4081 aaggttatca aacacgtatg aatgaaattt tacgagatgc tatgctaaaa gaattaaaaa
     4141 atcatcaata agaatgggag cttaggctcc catttttgac ttcattttta ggggggatta
     4201 ccattcaaaa atttaaatag tagattttat aaatttttaa gtgttatatt ataatataac
     4261 attaaatttg gttgttttat gctaaataaa agtaaactat ttttagcttt aattacttta
     4321 ggggcctcga aaattttatt ggcagctgaa gggcctgtta ctaccttaaa tacaatagta
     4381 ttaactgcac aatctgatga gttagggtct gagctgttag gcaaaagtct aaatgttagt
     4441 aatcagttta ttgatactag taaacttaaa cagcgttcga caactctagg ggatgccttg
     4501 ggtacggaat taggaattca ttctaatcaa tatggaggtg gagcatctac tcctattatt
     4561 cgtgggcagg aaggaaaacg tattaaagtt ttacagaata atgctgacgt gttggatatg
     4621 tctaatatgt ccccagatca cgctgtcaca gttgaaccaa gtttagctaa gtctattgaa
     4681 attatacgtg gtgcaagtac ccttttgtat agttcaaata gtgcggcagg ggtagtcaat
     4741 gtcattgact ataaaattcc aacccaaatg cctcaagacg gattagaggg taatactaca
     4801 ttacgtttta atactggcag taatgagaag ttgaccacag caggtgtaac agttggctta
     4861 agcccacatg tagctttacg ggcagaaggg ttatatcgta atgctggtaa ctataaaact
     4921 cctcattacc agtctagctc ttataattct ttagaagatc ttgaaaacca aaacagtatt
     4981 tataaaaatt taaaatattt accagaaagc tgggcagagt cgagagtagg tacgcttgga
     5041 ttatcctgga tagacgataa tacttattta ggagtttctt atacacatcg tcatgatgaa
     5101 tatggtttac cagcccatag ccatttatat gaaggttgtg gtgcctctgc aatcggtatc
     5161 gattcacgaa ttagtggatt aaaaaattat cttttatatt atcctcaatt gatggatgag
     5221 caagatatta attacataaa tccacgtcct gattgtcatc agcataatca tattcatgaa
     5281 actaatttct ctcataatgc tccttatatt gatttaaata ctcgacgtta tgatgtgcgt
     5341 ggagaattca ctcaaccatt taccggtatt gacaaaattc gtacctcatt gagttatata
     5401 gattattttc acaatgaact cgagggagat aagattacta acttttttaa aaatacagga
     5461 aaagtaggcc gcatagagtt atcacatcag ccattagggg aattaaccgg tatcttgggg
     5521 ctacaatatt tggaacagga taatagtgct ttatcaccag tccattctca agaaggccat
     5581 accacctatc tagataatca gcagctatta aatcgaaatg tcactaaaaa ctttagtgta
     5641 tttggcctag aaaaatataa ctggaacgac tttactttcg aactaggtgc tcgtatagaa
     5701 aagcagaaag ttagtatgga ttatgatata gaaaaaatta aagattcaat gaaaccttgg
     5761 cctaataaat ataacagtcc ttatgtagaa aaaaataata aaatcagggc tcaaaattta
     5821 aaatctattt tagaagctgt tcaacctaac aaagaaacag ctttttctta tgcaggtaca
     5881 gtccactgga gatttgctcc aaattatatc ttatcattaa caggtacaca tcaagaaaga
     5941 ttacctaatg cacaagaaat gtatactcat ggtatgcatc tcgctactaa ttcttttgaa
     6001 ataggtaacc gctttctaag aaaagagaaa tccaataatt tagaaatcag tctggcctat
     6061 aaggatgatt tacttgatta tcaaatcagt acttactatt atgattttga taactatata
     6121 tatttacaga cactcaatga agttttagga actacaaaag ttagagatca gcatacacta
     6181 cgtattaatc attatagcca gtctgctgct aatttttatg gattagaggg caacataggg
     6241 tatcaattta attctgttta tcacggctct ctttttggag attatgtgaa aggccgttta
     6301 acaaatttac ctgatgctgt aattgcatac gatatctgga atagagaacc gactttagct
     6361 ccccaaaagg atcgttacac tcctagatta cctcctgctc gtttgggtac acgactaaaa
     6421 gctgattttg atgaatcgct taaaggtgaa atagaatatt atcgagtctt taaacaagat
     6481 aatatttcta aatttgagca ggttacatca ggttataata tgcttaatat gactttagca
     6541 tataaaaata agctatctca cacagaatat gatttattct ttaaagcgaa taatttactt
     6601 gatcagaaag tctatgccca tgaaacattt ttgccatata ttccccaaat tggaagaaat
     6661 tttagtttag gcttaaatct taatttctag ataagatact gtaatcttct aagaaaattt
     6721 cttagaagat ttttcttata aaatgattag attaataatt aaggtaagcg tccgctgaat
     6781 cccataccta ttttttaatt cgatttaggt ttccagcagt gtggcagtaa ctcttcaatc
     6841 tgggtcactt tatgtgtcgg cagccttttc agcactgaat agccaccgat tttgtagaca
     6901 ccttttagct agataaaaag caattttttt gatctttaaa gaatgaggat ataagctgtt
     6961 taaaacttaa ttctaattgg ttaataggtg aaaaatgaaa ttagcagaag ctctcttact
     7021 tcgaagtgat caacaaaaaa aaattatttc actaaaacaa cgtatcaatg caaatgtatt
     7081 ggtacaagat ggtgatcaac catctgaaga tccaaatgaa ctactaaaac aagtattttc
     7141 tttaattcaa gaatttcaaa aattaagtta tgcaattcat gaaactaatg ctttaacaaa
     7201 attaaatgat ggccgttctc tacttgcttt attgactttg cgtgatgaat ttgtagaaca
     7261 gcacaaaacc ctaactgcag caataagtaa tacatctcgt gaatctgatc gttatagtac
     7321 gcgggaaata aagtggcata aagttattcc agtttcatca ttacaaaaac aagctgatga
     7381 tattagtctt aaattacgtg atttaaacgt gttaattcag tcgactaatt ggaaaattga
     7441 tttgattgaa gcctaatagt cctttagata taatcaagtt gaacatactg ggcgagtaca
     7501 agatccctta cttccacaac ataggattgt aaataaagtg tagtggcttg caaagcaagt
     7561 ggataagctt aaaattttaa gtacaaatca atccatcact tggcataact caaatctttg
     7621 tgcaaacaaa gttacacgtt acttataact accatgtact gacagtatgt tcacctcaat
     7681 tactaaaaat aaagaaatga acaagcatca gctcaactac atttctattt ttaattcagt
     7741 tcggcattta acataaaatc tcttatacga agcccaggtt aattctgggc tttttgttca
     7801 atctcatcat tggccgagaa catacttgga ttcgtaagcc tgatattttc ctgacaactt
     7861 ccacacagca tcaatcctgc aaaaagttgt acattttgtc tattttcaca aagcgtacat
     7921 ggctttgaaa gctgaataat attatctgac actactttac cctcttatag ttatcatctg
     7981 catatgaata acctttagct tgtatcattc taacctcatc tttagaagcg ccatttttca
     8041 ttaagagcca caacatcatg aaatatagtc cagaaaagtc ttcatagtat ttttcagaaa
     8101 aattgttatc ttcctcaaaa tcatctattc gcttttctaa tcgattcacc agatcctcaa
     8161 tacgatcggc ttgctgttta acagcaatag attgaaaatc attggtcaaa attttactga
     8221 ataatttact ggttctgatt ccttctgaat tagccaattg atcgtagaat tctgctactt
     8281 gtggatctaa cctgactgca taagttttga ctttttcgcc cataaattac acctcttatt
     8341 aaaattagat tttgtacaaa attaaatata tataattcaa ctaattaaat aaaaaataat
     8401 aattagatta cgtacaaact tatttgtaca gaattaattt attctaaaac aaaaacttat
     8461 ttaaatataa tgcttttttt atgtacaatc atgagtacat attttttctt aaatttatca
     8521 atatcttatc taaaaaaagt gttgttttga tgtacaacca tgagcacagc ataaaaaata
     8581 gataattcaa tcacttattc taaaaaaaga ccatttttct gtacacgttt ctgagaccct
     8641 aaaaaatcgc tgaaatcctt atcctgtaag gctttgaggg gtgtcatcgt tagatgcgtg
     8701 gtgtgtgaca aaaaaatccc ggcatgtgcc ggatcttgga tt
//
