LOCUS       NZ_CP121580             8848 bp    DNA     circular CON 17-MAY-2024
DEFINITION  Acinetobacter baumannii strain RAB53 plasmid pI_RAB53, complete
            sequence.
ACCESSION   NZ_CP121580
VERSION     NZ_CP121580.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27010277
            Assembly: GCF_029713905.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 8848)
  AUTHORS   Alghorbi,M.F., Bawazeer,R., Okdah,L., Alswaji,A., Alzayer,M.A.,
            Alrashidi,E., Alshahrani,A.S., Al Johani,S.M., NGHA Surveillance
            Group,N.S.G., Balkhy,H. and Doumith,M.
  TITLE     Antimicrobial resistance and genomic characterisation of
            Acinetobacter baumannii clinical isolates from a nation
            representative surveillance in Saudi Arabia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8848)
  AUTHORS   Alghorbi,M.F., Bawazeer,R., Okdah,L., Alswaji,A., Alzayer,M.A.,
            Alrashidi,E., Alshahrani,A.S., Al Johani,S.M., NGHA Surveillance
            Group,N.S.G., Balkhy,H. and Doumith,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-APR-2023) Infectious Diseases Research, King Abdullah
            International Medical Research Center (KAIMRC), KAIMRC, P.O Box
            22490, Riyadh 11426, Saudi Arabia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: EToKi v. 1.2
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 207x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029713905.1-RS_2024_05_17
            Annotation Date                   :: 05/17/2024 04:54:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,856
            CDSs (total)                      :: 3,761
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 47 of 154
            Pseudo Genes (incomplete)         :: 96 of 154
            Pseudo Genes (internal stop)      :: 20 of 154
            Pseudo Genes (multiple problems)  :: 9 of 154
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP121580.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8848
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="RAB53"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pI_RAB53"
                     /geo_loc_name="Saudi Arabia:Riyadh"
                     /lat_lon="24.7136 N 46.6753 E"
                     /collection_date="2019"
                     /collected_by="KAIMRC AMR WGS_Based Surveillance Program"
     gene            complement(169..483)
                     /locus_tag="MTS70_RS19280"
                     /old_locus_tag="MTS70_19280"
     CDS             complement(169..483)
                     /locus_tag="MTS70_RS19280"
                     /old_locus_tag="MTS70_19280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            complement(470..757)
                     /locus_tag="MTS70_RS19285"
                     /old_locus_tag="MTS70_19285"
     CDS             complement(470..757)
                     /locus_tag="MTS70_RS19285"
                     /old_locus_tag="MTS70_19285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438826.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            1130..1648
                     /locus_tag="MTS70_RS19290"
                     /old_locus_tag="MTS70_19290"
     CDS             1130..1648
                     /locus_tag="MTS70_RS19290"
                     /old_locus_tag="MTS70_19290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447193.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_000447193.1"
                     /translation="METFAEPVFSNDLLAKAENGDTSAQLELAEIYLYGHGVDSDENQA
                     EVWALKSAENGNVAAMFWLADGYVTYARLMEDDDKNDSLEHFQKAFKWFQKASENGHSE
                     SMVELADLYTRADSGIEVNINKAFELREKAAKLGNKKAMRSLSVMYRDGIGIPKNTDLA
                     QSWWDKSEN"
                     /name="tetratricopeptide repeat protein"
     gene            complement(1837..1980)
                     /locus_tag="MTS70_RS19295"
                     /old_locus_tag="MTS70_19295"
     CDS             complement(1837..1980)
                     /locus_tag="MTS70_RS19295"
                     /old_locus_tag="MTS70_19295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            complement(2000..2575)
                     /locus_tag="MTS70_RS19300"
                     /old_locus_tag="MTS70_19300"
     CDS             complement(2000..2575)
                     /locus_tag="MTS70_RS19300"
                     /old_locus_tag="MTS70_19300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_243327585.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTSQKIESSKQEGDESKKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATAAPHQDATACDTKMPQHATTQQDTDNKKNNELNIAENVAVPQQETTAYHTQTLQH
                     ATLQSVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(2568..3518)
                     /gene="repM"
                     /locus_tag="MTS70_RS19305"
                     /old_locus_tag="MTS70_19305"
     CDS             complement(2568..3518)
                     /gene="repM"
                     /locus_tag="MTS70_RS19305"
                     /old_locus_tag="MTS70_19305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001205343.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHADSYINQFGVHRNTAYQALKDACKDLFARQFSYQEKKANGNIRNVMSRWVSQIAY
                     NDNEATVDLIFAPAVVPFITRLEEQFTKYELQQVSSLSSAYAIRLYELLIQWRSTGKTP
                     TIELQEFRKKLGVLDNEYLRMAHLKERVLELSIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDSPSIERDPNTLELFSKMTDAQRHMFANKLSELPEMGRYSQGTESYQQFAV
                     RIAEMLQDPAQFKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            4202..4456
                     /locus_tag="MTS70_RS19350"
     CDS             4202..4456
                     /locus_tag="MTS70_RS19350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001014303.1"
                     /translation="MNGQKIDFLRFSKQGNKYFFPCKTKHRPYVTLANFPIQDPAHAGI
                     FLSHTTHLTMTPLKALQDKDFSDFLGSQKRVQKNGLFLE"
                     /name="hypothetical protein"
     gene            4728..5099
                     /locus_tag="MTS70_RS19310"
                     /old_locus_tag="MTS70_19310"
     CDS             4728..5099
                     /locus_tag="MTS70_RS19310"
                     /old_locus_tag="MTS70_19310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000504218.1"
                     /translation="MGEKVKTYAVRLDPQVAEFYDQLANSEGIRTSKLFSKILTNDFQS
                     IAVKQQADRIEDLVNRLEKRIDDFEEDNNFSEKYYEDFSGLYFMMLWLLMKNGASKDEV
                     RMIQAKGYSYADDNYKRVK"
                     /name="hypothetical protein"
     gene            5099..5272
                     /locus_tag="MTS70_RS19315"
                     /old_locus_tag="MTS70_19315"
     CDS             5099..5272
                     /locus_tag="MTS70_RS19315"
                     /old_locus_tag="MTS70_19315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001282484.1"
                     /translation="MSDNIIQLSKPCTLCENRQNVQLFAGLMLCGSCQENIRLTNPSMF
                     SANDEIEQKAQN"
                     /name="hypothetical protein"
     gene            complement(5595..6056)
                     /locus_tag="MTS70_RS19320"
                     /old_locus_tag="MTS70_19320"
     CDS             complement(5595..6056)
                     /locus_tag="MTS70_RS19320"
                     /old_locus_tag="MTS70_19320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(6361..8772)
                     /gene="znuD2"
                     /locus_tag="MTS70_RS19325"
                     /old_locus_tag="MTS70_19325"
     CDS             complement(6361..8772)
                     /gene="znuD2"
                     /locus_tag="MTS70_RS19325"
                     /old_locus_tag="MTS70_19325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     Typing          complement(2569..3518)
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaagtaatta aagctaaaaa tagtttactt ttatttagca taaaacaacc aaatttaatg
       61 ttatattata atataacact taaaaattta taaaatctac tatttaaatt tttgaatggt
      121 aatcccccct aaaaatgaag tcaaaaatgg gagcctaagc tcccattctt attgatgatt
      181 ttttaattct tttagcatag catctcgtaa aatttcattc atacgtgttt gataaccttt
      241 gccttgtgct ttgaaccaag caagtacatc agcatctaac cgaattgaag tttgttgctt
      301 tactgggcgg taaaattgat tttggcgtac agcattgctc cagtctgtaa tttcaggaat
      361 atctgataga tctagctgat catcaggaac cgtgccttta gcaagcaagc gttgaatttc
      421 agcatcttgc ttctcaccaa atttctcatt cagctctttg tgtgagtatc taaccatgct
      481 catatttgtt tcgctccgct ttagtgactt gccttgcact aatgattcgg atgatttcac
      541 agtcatcttc atcaaaaatg gtgtgagcca ccaataacat tagtacgcct ttaactcgtc
      601 caatggtttg ccaacgttct tcaccattgg tatgtctgtc ttggattgag atccgtaatg
      661 gatcttcaaa aacaaggctt gcagtttcga aagagatatc atgctttttc tgattctttc
      721 gattcttagc ctcatcccat tcaaaatact gttccataaa aaacattcaa atttgtatat
      781 acaataatgt atcacaaaat tgtatcactc aaccagattc gtgtaatttt tagtcaaaat
      841 tattggcaag aagtatcaaa actgctcata aaaaagccga cttgtttcaa agtcggcttt
      901 ttgggcattt tgggattttt ctatttttat taagtagttg atatttatat taatatacaa
      961 gtgtacttcg tataatcgcc attatgttaa atagagataa gacatactct aattttagag
     1021 ttttctttta ggtatgatat aaaaatatac aagcctaaca gtttagttta aaggttaaaa
     1081 attgaataaa ttaacaaata tttttatggc tttaggagtt tgcctttcaa tggaaacatt
     1141 tgcagaacct gttttttcta atgatttatt agcgaaagca gaaaatggag atacttctgc
     1201 tcagttagaa ttagccgaga tttatctata tggtcatggt gttgattcag atgaaaatca
     1261 agctgaagtt tgggctctta aatcagcaga aaatggaaat gtagcagcaa tgttttggtt
     1321 agctgatgga tatgttactt atgctagatt aatggaagat gatgacaaaa acgattcttt
     1381 agaacatttc caaaaagctt ttaagtggtt tcagaaagcc tcagaaaatg gccattctga
     1441 atcaatggtt gagttagctg atctatatac tcgtgcagat agcggaatag aagttaatat
     1501 taataaagct tttgaacttc gtgaaaaggc tgcaaagtta ggtaataaga aagcaatgcg
     1561 aagtctttcc gttatgtatc gtgatggtat aggtattcct aaaaatactg atttagctca
     1621 aagttggtgg gataagtctg aaaattagtt ttaatatttc ctaaaattaa cataatacac
     1681 cttatacgaa atgctgtata attatcctta taattcaacg ctgtagtttc tagtttatag
     1741 cccgagcatc cctgttcggg ctttttttat gacttttcac gttccacgct attatttcat
     1801 caatgttcca cacttttgat tagctcaatt gaataatcag gatgcctggt ctttttcttt
     1861 ttggcattca tccagctctt tcttcagcgc ctttttatcc tggtacgatg caatcagcat
     1921 gatcgggatc agcagcggag ctacaataat tcccagggca atgtctttca gtttcggcat
     1981 gatctttcac ccttattgct cagtcattat caaagacggc attcagcaca cggccaaata
     2041 aaccacgttt tttgtgttgt ggcactgcca cactttgcaa cgtggcatgc tgtagcgttt
     2101 gggtgtggta tgccgtagtt tcttgctgtg gcactgctac attttctgca atatttaatt
     2161 cattattctt tttgttatca gtgtcttgtt gtgttgtcgc atgctgtggc atcttggtgt
     2221 cgcatgccgt agcatcttgg tgtggtgctg ctgtggcagg tgcaacactt ttcagctcaa
     2281 tgagtgtttg catacggtca atttgttgca ttaaccgttc ttcacgttct tggtattttt
     2341 tgacttcatt ttctaaatgg gcaataagtt tttttttaga ttcatcacct tcttgcttag
     2401 atgattcaat tttttgagat gtttgctcag gttctccaaa gcatcgtatg acctctgcta
     2461 gatcgaattg tccatcactg gagcgtgata aacgtccatt acgtacagct ctgtaaatgg
     2521 ttgatctatc cacatgaaat tttttagata cttcacttag tgataactta gccatttacg
     2581 gtgtcctttt tatttgatgg catgtatccc acttttttta ggtatgggta tagctctttg
     2641 aattgagcgg gatcttgtag catctcagca atacgtacag caaactgttg gtagctttct
     2701 gttccttgtg aatagcgacc catttcaggg agttctgaaa gtttatttgc aaacatatgc
     2761 cgttgagcat cggtcatctt tgaaaaaagc tccaaagtgt tcggatctct ttctattgat
     2821 gggctatcct tcttcttctg tttaaaggta aaagaaaatc ctgaaataga acgtcctctt
     2881 ttatgctgtt catattttac agttatatcc gtatgctcat ttatttgttt aattgaaagc
     2941 tctaaaacac gctcttttaa atgagccatc cgtaaatatt cattatcaag aacgcctaac
     3001 ttctttctaa attcttgtag ttctatggtt ggggttttac cagtgcttcg ccactgaatt
     3061 agaagctcat ataagcgaat ggcataagcg ctactaagac tactaacttg ctgtaattca
     3121 tatttagtaa attgttcttc cagtcgggtt atgaagggaa caacagcagg tgcaaatatt
     3181 aagtctacag ttgcttcatt gtcgttataa gcaatttgag atacccaacg actcataaca
     3241 tttcggatat tcccattagc ttttttctct tgatagctaa attgacgcgc gaataaatcc
     3301 ttacaagcat cttttaaggc ttgataagct gtattacggt gtacaccaaa ttgattgata
     3361 taactatctg catgaacttc taatggatca tttgcattaa tccctttccc actttccctt
     3421 gcttcaacaa tagccaataa aattaaacgt tgttctacta agtctaagtt atagcttgcg
     3481 ttgattaagg cattgtcctt tacaactaaa tctctcatag ccactatttt ttattgtgta
     3541 taagtacacg ttaatattat ttgtgtacta atgcaaggta aacgtggaca tattgcaagg
     3601 taaacgtgga catattgcaa ggtaaacgtg gacatattgc aaggtaaacg tggacatata
     3661 agagctaaaa ccctttattc ataaggcttt caagccattt aaaagtattt aaaaacttta
     3721 aaataattaa aagcccaggg aatgaaaaat tgcccttggt tttcgcttcg ctcaaactct
     3781 attgaacttc gctttcgctc agttcgttgg ggcaattttt tggttaatac tggcgtgact
     3841 ttaaaaaaaa gcaaaagaaa cttggaattc gcttcgctca taaaactttt ttactcgcta
     3901 cgctcgatcc aggagcaaat ttctgcacga aacttacgct ttcttaggct aaagaggaca
     3961 aaaaaatgtt tttcagaggg tctaggcgca aattttgata gttcgctcgt agacactcgc
     4021 tctatgcatc tatctaaagt caaaatacct ctttatttcg ctctcatttg cgttttttga
     4081 gctgttttgt gtttttcact atgagatcta tgttttctag gtaaaggctc ttaaatcgca
     4141 tttgaacgcg ttctatgaag cttttcataa ttaaagtttt gggttgaaat ttttaacctg
     4201 gatgaatgga caaaaaattg attttttacg tttttctaaa caaggaaata aatacttttt
     4261 tccatgcaaa accaagcacc ggccctacgt cactttagcc aattttccaa tccaagatcc
     4321 ggcacatgcc gggatttttt tgtcacacac cacgcatcta acgatgacac ccctcaaagc
     4381 cttacaggat aaggatttca gcgatttttt agggtctcag aaacgtgtac agaaaaatgg
     4441 tcttttttta gaataagtga ttgaattatc tattttttat gctgtgctca tggttgtaca
     4501 tcaaaacaac acttttttta gataagatat tgataaattt aagaaaaaat atgtactcat
     4561 gattgtacat aaaaaaagca ttatatttaa ataagttttt gttttagaat aaattaattc
     4621 tgtacaaata agtttgtacg taatctaatt attatttttt atttaattag ttgaattata
     4681 tatatttaat tttgtacaaa atctaatttt aataagaggt gtaatttatg ggcgaaaaag
     4741 tcaaaactta tgcagtcagg ttagatccac aagtagcaga attctacgat caattggcta
     4801 attcagaagg aatcagaacc agtaaattat tcagtaaaat tttgaccaat gattttcaat
     4861 ctattgctgt taaacagcaa gccgatcgta ttgaggatct ggtgaatcga ttagaaaagc
     4921 gaatagatga ttttgaggaa gataacaatt tttctgaaaa atactatgaa gacttttctg
     4981 gactatattt catgatgttg tggctcttaa tgaaaaatgg cgcttctaaa gatgaggtta
     5041 gaatgataca agctaaaggt tattcatatg cagatgataa ctataagagg gtaaagtagt
     5101 gtcagataat attattcagc tttcaaagcc atgtacgctt tgtgaaaata gacaaaatgt
     5161 acaacttttt gcaggattga tgctgtgtgg aagttgtcag gaaaatatca ggcttacgaa
     5221 tccaagtatg ttctcggcca atgatgagat tgaacaaaaa gcccagaatt aacctgggct
     5281 tcgtataaga gattttatgt taaatgccga actgaattaa aaatagaaat gtagttgagc
     5341 tgatgcttgt tcatttcttt atttttagta attgaggtga acatactgtc agtacatggt
     5401 agttataagt aacgtgtaac tttgtttgca caaagatttg agttatgcca agtgatggat
     5461 tgatttgtac ttaaaatttt aagcttatcc acttgctttg caagccacta cactttattt
     5521 acaatcctat gttgtggaag taagggatct tgtactcgcc cagtatgttc aacttgatta
     5581 tatctaaagg actattaggc ttcaatcaaa tcaattttcc aattagtcga ctgaattaac
     5641 acgtttaaat cacgtaattt aagactaata tcatcagctt gtttttgtaa tgatgaaact
     5701 ggaataactt tatgccactt tatttcccgc gtactataac gatcagattc acgagatgta
     5761 ttacttattg ctgcagttag ggttttgtgc tgttctacaa attcatcacg caaagtcaat
     5821 aaagcaagta gagaacggcc atcatttaat tttgttaaag cattagtttc atgaattgca
     5881 taacttaatt tttgaaattc ttgaattaaa gaaaatactt gttttagtag ttcatttgga
     5941 tcttcagatg gttgatcacc atcttgtacc aatacatttg cattgatacg ttgttttagt
     6001 gaaataattt ttttttgttg atcacttcga agtaagagag cttctgctaa tttcattttt
     6061 cacctattaa ccaattagaa ttaagtttta aacagcttat atcctcattc tttaaagatc
     6121 aaaaaaattg ctttttatct agctaaaagg tgtctacaaa atcggtggct attcagtgct
     6181 gaaaaggctg ccgacacata aagtgaccca gattgaagag ttactgccac actgctggaa
     6241 acctaaatcg aattaaaaaa taggtatggg attcagcgga cgcttacctt aattattaat
     6301 ctaatcattt tataagaaaa atcttctaag aaattttctt agaagattac agtatcttat
     6361 ctagaaatta agatttaagc ctaaactaaa atttcttcca atttggggaa tatatggcaa
     6421 aaatgtttca tgggcataga ctttctgatc aagtaaatta ttcgctttaa agaataaatc
     6481 atattctgtg tgagatagct tatttttata tgctaaagtc atattaagca tattataacc
     6541 tgatgtaacc tgctcaaatt tagaaatatt atcttgttta aagactcgat aatattctat
     6601 ttcaccttta agcgattcat caaaatcagc ttttagtcgt gtacccaaac gagcaggagg
     6661 taatctagga gtgtaacgat ccttttgggg agctaaagtc ggttctctat tccagatatc
     6721 gtatgcaatt acagcatcag gtaaatttgt taaacggcct ttcacataat ctccaaaaag
     6781 agagccgtga taaacagaat taaattgata ccctatgttg ccctctaatc cataaaaatt
     6841 agcagcagac tggctataat gattaatacg tagtgtatgc tgatctctaa cttttgtagt
     6901 tcctaaaact tcattgagtg tctgtaaata tatatagtta tcaaaatcat aatagtaagt
     6961 actgatttga taatcaagta aatcatcctt ataggccaga ctgatttcta aattattgga
     7021 tttctctttt cttagaaagc ggttacctat ttcaaaagaa ttagtagcga gatgcatacc
     7081 atgagtatac atttcttgtg cattaggtaa tctttcttga tgtgtacctg ttaatgataa
     7141 gatataattt ggagcaaatc tccagtggac tgtacctgca taagaaaaag ctgtttcttt
     7201 gttaggttga acagcttcta aaatagattt taaattttga gccctgattt tattattttt
     7261 ttctacataa ggactgttat atttattagg ccaaggtttc attgaatctt taattttttc
     7321 tatatcataa tccatactaa ctttctgctt ttctatacga gcacctagtt cgaaagtaaa
     7381 gtcgttccag ttatattttt ctaggccaaa tacactaaag tttttagtga catttcgatt
     7441 taatagctgc tgattatcta gataggtggt atggccttct tgagaatgga ctggtgataa
     7501 agcactatta tcctgttcca aatattgtag ccccaagata ccggttaatt cccctaatgg
     7561 ctgatgtgat aactctatgc ggcctacttt tcctgtattt ttaaaaaagt tagtaatctt
     7621 atctccctcg agttcattgt gaaaataatc tatataactc aatgaggtac gaattttgtc
     7681 aataccggta aatggttgag tgaattctcc acgcacatca taacgtcgag tatttaaatc
     7741 aatataagga gcattatgag agaaattagt ttcatgaata tgattatgct gatgacaatc
     7801 aggacgtgga tttatgtaat taatatcttg ctcatccatc aattgaggat aatataaaag
     7861 ataatttttt aatccactaa ttcgtgaatc gataccgatt gcagaggcac cacaaccttc
     7921 atataaatgg ctatgggctg gtaaaccata ttcatcatga cgatgtgtat aagaaactcc
     7981 taaataagta ttatcgtcta tccaggataa tccaagcgta cctactctcg actctgccca
     8041 gctttctggt aaatatttta aatttttata aatactgttt tggttttcaa gatcttctaa
     8101 agaattataa gagctagact ggtaatgagg agttttatag ttaccagcat tacgatataa
     8161 cccttctgcc cgtaaagcta catgtgggct taagccaact gttacacctg ctgtggtcaa
     8221 cttctcatta ctgccagtat taaaacgtaa tgtagtatta ccctctaatc cgtcttgagg
     8281 catttgggtt ggaattttat agtcaatgac attgactacc cctgccgcac tatttgaact
     8341 atacaaaagg gtacttgcac cacgtataat ttcaatagac ttagctaaac ttggttcaac
     8401 tgtgacagcg tgatctgggg acatattaga catatccaac acgtcagcat tattctgtaa
     8461 aactttaata cgttttcctt cctgcccacg aataatagga gtagatgctc cacctccata
     8521 ttgattagaa tgaattccta attccgtacc caaggcatcc cctagagttg tcgaacgctg
     8581 tttaagttta ctagtatcaa taaactgatt actaacattt agacttttgc ctaacagctc
     8641 agaccctaac tcatcagatt gtgcagttaa tactattgta tttaaggtag taacaggccc
     8701 ttcagctgcc aataaaattt tcgaggcccc taaagtaatt aaagctaaaa atagtttact
     8761 tttatttagc ataaaacaac caaatttaat gttatattat aatataacac ttaaaaattt
     8821 ataaaatcta ctatttaaat ttttgaat
//
