LOCUS       NZ_CP121566             8763 bp    DNA     circular CON 17-MAY-2024
DEFINITION  Acinetobacter baumannii strain RAB94 plasmid pI_RAB94, complete
            sequence.
ACCESSION   NZ_CP121566
VERSION     NZ_CP121566.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27010289
            Assembly: GCF_029713785.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 8763)
  AUTHORS   Alghorbi,M.F., Bawazeer,R., Okdah,L., Alswaji,A., Alzayer,M.A.,
            Alrashidi,E., Alshahrani,A.S., Al Johani,S.M., NGHA Surveillance
            Group,N.S.G., Balkhy,H. and Doumith,M.
  TITLE     Antimicrobial resistance and genomic characterisation of
            Acinetobacter baumannii clinical isolates from a nation
            representative surveillance in Saudi Arabia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8763)
  AUTHORS   Alghorbi,M.F., Bawazeer,R., Okdah,L., Alswaji,A., Alzayer,M.A.,
            Alrashidi,E., Alshahrani,A.S., Al Johani,S.M., NGHA Surveillance
            Group,N.S.G., Balkhy,H. and Doumith,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-APR-2023) Infectious Diseases Research, King Abdullah
            International Medical Research Center (KAIMRC), KAIMRC, P.O Box
            22490, Riyadh 11426, Saudi Arabia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: EToKi v. 1.2
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 142x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029713785.1-RS_2024_05_17
            Annotation Date                   :: 05/17/2024 05:10:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,916
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 94
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 27 of 88
            Pseudo Genes (incomplete)         :: 48 of 88
            Pseudo Genes (internal stop)      :: 18 of 88
            Pseudo Genes (multiple problems)  :: 5 of 88
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP121566.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8763
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="RAB94"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pI_RAB94"
                     /geo_loc_name="Saudi Arabia:Riyadh"
                     /lat_lon="24.7136 N 46.6753 E"
                     /collection_date="2019"
                     /collected_by="KAIMRC AMR WGS_Based Surveillance Program"
     gene            153..614
                     /locus_tag="MTS31_RS19580"
                     /old_locus_tag="MTS31_19580"
     CDS             153..614
                     /locus_tag="MTS31_RS19580"
                     /old_locus_tag="MTS31_19580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(937..1110)
                     /locus_tag="MTS31_RS19585"
                     /old_locus_tag="MTS31_19585"
     CDS             complement(937..1110)
                     /locus_tag="MTS31_RS19585"
                     /old_locus_tag="MTS31_19585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001282484.1"
                     /translation="MSDNIIQLSKPCTLCENRQNVQLFAGLMLCGSCQENIRLTNPSMF
                     SANDEIEQKAQN"
                     /name="hypothetical protein"
     gene            complement(1110..1481)
                     /locus_tag="MTS31_RS19590"
                     /old_locus_tag="MTS31_19590"
     CDS             complement(1110..1481)
                     /locus_tag="MTS31_RS19590"
                     /old_locus_tag="MTS31_19590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000504218.1"
                     /translation="MGEKVKTYAVRLDPQVAEFYDQLANSEGIRTSKLFSKILTNDFQS
                     IAVKQQADRIEDLVNRLEKRIDDFEEDNNFSEKYYEDFSGLYFMMLWLLMKNGASKDEV
                     RMIQAKGYSYADDNYKRVK"
                     /name="hypothetical protein"
     gene            complement(1753..2007)
                     /locus_tag="MTS31_RS19660"
     CDS             complement(1753..2007)
                     /locus_tag="MTS31_RS19660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001014303.1"
                     /translation="MNGQKIDFLRFSKQGNKYFFPCKTKHRPYVTLANFPIQDPAHAGI
                     FLSHTTHLTMTPLKALQDKDFSDFLGSQKRVQKNGLFLE"
                     /name="hypothetical protein"
     gene            2691..3641
                     /gene="repM"
                     /locus_tag="MTS31_RS19595"
                     /old_locus_tag="MTS31_19595"
     CDS             2691..3641
                     /gene="repM"
                     /locus_tag="MTS31_RS19595"
                     /old_locus_tag="MTS31_19595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001205343.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHADSYINQFGVHRNTAYQALKDACKDLFARQFSYQEKKANGNIRNVMSRWVSQIAY
                     NDNEATVDLIFAPAVVPFITRLEEQFTKYELQQVSSLSSAYAIRLYELLIQWRSTGKTP
                     TIELQEFRKKLGVLDNEYLRMAHLKERVLELSIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDSPSIERDPNTLELFSKMTDAQRHMFANKLSELPEMGRYSQGTESYQQFAV
                     RIAEMLQDPAQFKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            3634..4209
                     /locus_tag="MTS31_RS19600"
                     /old_locus_tag="MTS31_19600"
     CDS             3634..4209
                     /locus_tag="MTS31_RS19600"
                     /old_locus_tag="MTS31_19600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_001096616.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTSQKIESSKQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATAAPHQDATACDTKMPQHATTQQDTDNKKNNELNIAENVAVPQQETTAYHTQTLQH
                     ATLQSVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            4229..4372
                     /locus_tag="MTS31_RS19605"
                     /old_locus_tag="MTS31_19605"
     CDS             4229..4372
                     /locus_tag="MTS31_RS19605"
                     /old_locus_tag="MTS31_19605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            complement(4561..5079)
                     /locus_tag="MTS31_RS19610"
                     /old_locus_tag="MTS31_19610"
     CDS             complement(4561..5079)
                     /locus_tag="MTS31_RS19610"
                     /old_locus_tag="MTS31_19610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447193.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_000447193.1"
                     /translation="METFAEPVFSNDLLAKAENGDTSAQLELAEIYLYGHGVDSDENQA
                     EVWALKSAENGNVAAMFWLADGYVTYARLMEDDDKNDSLEHFQKAFKWFQKASENGHSE
                     SMVELADLYTRADSGIEVNINKAFELREKAAKLGNKKAMRSLSVMYRDGIGIPKNTDLA
                     QSWWDKSEN"
                     /name="tetratricopeptide repeat protein"
     gene            5452..5739
                     /locus_tag="MTS31_RS19615"
                     /old_locus_tag="MTS31_19615"
     CDS             5452..5739
                     /locus_tag="MTS31_RS19615"
                     /old_locus_tag="MTS31_19615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438826.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            5726..6040
                     /locus_tag="MTS31_RS19620"
                     /old_locus_tag="MTS31_19620"
     CDS             5726..6040
                     /locus_tag="MTS31_RS19620"
                     /old_locus_tag="MTS31_19620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            6168..8579
                     /gene="znuD2"
                     /locus_tag="MTS31_RS19625"
                     /old_locus_tag="MTS31_19625"
     CDS             6168..8579
                     /gene="znuD2"
                     /locus_tag="MTS31_RS19625"
                     /old_locus_tag="MTS31_19625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     Typing          2692..3641
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggtcacttta tgtgtcggca gccttttcag cactgaatag ccaccgattt tgtagacacc
       61 ttttagctag ataaaaagca atttttttga tctttaaaga atgaggatat aagctgttta
      121 aaacttaatt ctaattggtt aataggtgaa aaatgaaatt agcagaagct ctcttacttc
      181 gaagtgatca acaaaaaaaa attatttcac taaaacaacg tatcaatgca aatgtattgg
      241 tacaagatgg tgatcaacca tctgaagatc caaatgaact actaaaacaa gtattttctt
      301 taattcaaga atttcaaaaa ttaagttatg caattcatga aactaatgct ttaacaaaat
      361 taaatgatgg ccgttctcta cttgctttat tgactttgcg tgatgaattt gtagaacagc
      421 acaaaaccct aactgcagca ataagtaata catctcgtga atctgatcgt tatagtacgc
      481 gggaaataaa gtggcataaa gttattccag tttcatcatt acaaaaacaa gctgatgata
      541 ttagtcttaa attacgtgat ttaaacgtgt taattcagtc gactaattgg aaaattgatt
      601 tgattgaagc ctaatagtcc tttagatata atcaagttga acatactggg cgagtacaag
      661 atcccttact tccacaacat aggattgtaa ataaagtgta gtggcttgca aagcaagtgg
      721 ataagcttaa aattttaagt acaaatcaat ccatcacttg gcataactca aatctttgtg
      781 caaacaaagt tacacgttac ttataactac catgtactga cagtatgttc acctcaatta
      841 ctaaaaataa agaaatgaac aagcatcagc tcaactacat ttctattttt aattcagttc
      901 ggcatttaac ataaaatctc ttatacgaag cccaggttaa ttctgggctt tttgttcaat
      961 ctcatcattg gccgagaaca tacttggatt cgtaagcctg atattttcct gacaacttcc
     1021 acacagcatc aatcctgcaa aaagttgtac attttgtcta ttttcacaaa gcgtacatgg
     1081 ctttgaaagc tgaataatat tatctgacac tactttaccc tcttatagtt atcatctgca
     1141 tatgaataac ctttagcttg tatcattcta acctcatctt tagaagcgcc atttttcatt
     1201 aagagccaca acatcatgaa atatagtcca gaaaagtctt catagtattt ttcagaaaaa
     1261 ttgttatctt cctcaaaatc atctattcgc ttttctaatc gattcaccag atcctcaata
     1321 cgatcggctt gctgtttaac agcaatagat tgaaaatcat tggtcaaaat tttactgaat
     1381 aatttactgg ttctgattcc ttctgaatta gccaattgat cgtagaattc tgctacttgt
     1441 ggatctaacc tgactgcata agttttgact ttttcgccca taaattacac ctcttattaa
     1501 aattagattt tgtacaaaat taaatatata taattcaact aattaaataa aaaataataa
     1561 ttagattacg tacaaactta tttgtacaga attaatttat tctaaaacaa aaacttattt
     1621 aaatataatg ctttttttat gtacaatcat gagtacatat tttttcttaa atttatcaat
     1681 atcttatcta aaaaaagtgt tgttttgatg tacaaccatg agcacagcat aaaaaataga
     1741 taattcaatc acttattcta aaaaaagacc atttttctgt acacgtttct gagaccctaa
     1801 aaaatcgctg aaatccttat cctgtaaggc tttgaggggt gtcatcgtta gatgcgtggt
     1861 gtgtgacaaa aaaatcccgg catgtgccgg atcttggatt ggaaaattgg ctaaagtgac
     1921 gtagggccgg tgcttggttt tgcatggaaa aaagtattta tttccttgtt tagaaaaacg
     1981 taaaaaatca attttttgtc cattcatcca ggttaaaaat ttcaacccaa aactttaatt
     2041 atgaaaagct tcatagaacg cgttcaaatg cgatttaaga gcctttacct agaaaacata
     2101 gatctcatag tgaaaaacac aaaacagctc aaaaaacgca aatgagagcg aaataaagag
     2161 gtattttgac tttagataga tgcatagagc gagtgtctac gagcgaacta tcaaaatttg
     2221 cgcctagacc ctctgaaaaa catttttttg tcctctttag cctaagaaag cgtaagtttc
     2281 gtgcagaaat ttgctcctgg atcgagcgta gcgagtaaaa aagttttatg agcgaagcga
     2341 attccaagtt tcttttgctt ttttttaaag tcacgccagt attaaccaaa aaattgcccc
     2401 aacgaactga gcgaaagcga agttcaatag agtttgagcg aagcgaaaac caagggcaat
     2461 ttttcattcc ctgggctttt aattatttta aagtttttaa atacttttaa atggcttgaa
     2521 agccttatga ataaagggtt ttagctctta tatgtccacg tttaccttgc aatatgtcca
     2581 cgtttacctt gcaatatgtc cacgtttacc ttgcaatatg tccacgttta ccttgcatta
     2641 gtacacaaat aatattaacg tgtacttata cacaataaaa aatagtggct atgagagatt
     2701 tagttgtaaa ggacaatgcc ttaatcaacg caagctataa cttagactta gtagaacaac
     2761 gtttaatttt attggctatt gttgaagcaa gggaaagtgg gaaagggatt aatgcaaatg
     2821 atccattaga agttcatgca gatagttata tcaatcaatt tggtgtacac cgtaatacag
     2881 cttatcaagc cttaaaagat gcttgtaagg atttattcgc gcgtcaattt agctatcaag
     2941 agaaaaaagc taatgggaat atccgaaatg ttatgagtcg ttgggtatct caaattgctt
     3001 ataacgacaa tgaagcaact gtagacttaa tatttgcacc tgctgttgtt cccttcataa
     3061 cccgactgga agaacaattt actaaatatg aattacagca agttagtagt cttagtagcg
     3121 cttatgccat tcgcttatat gagcttctaa ttcagtggcg aagcactggt aaaaccccaa
     3181 ccatagaact acaagaattt agaaagaagt taggcgttct tgataatgaa tatttacgga
     3241 tggctcattt aaaagagcgt gttttagagc tttcaattaa acaaataaat gagcatacgg
     3301 atataactgt aaaatatgaa cagcataaaa gaggacgttc tatttcagga ttttctttta
     3361 cctttaaaca gaagaagaag gatagcccat caatagaaag agatccgaac actttggagc
     3421 ttttttcaaa gatgaccgat gctcaacggc atatgtttgc aaataaactt tcagaactcc
     3481 ctgaaatggg tcgctattca caaggaacag aaagctacca acagtttgct gtacgtattg
     3541 ctgagatgct acaagatccc gctcaattca aagagctata cccataccta aaaaaagtgg
     3601 gatacatgcc atcaaataaa aaggacaccg taaatggcta agttatcact aagtgaagta
     3661 tctaaaaaat ttcatgtgga tagatcaacc atttacagag ctgtacgtaa tggacgttta
     3721 tcacgctcca gtgatggaca attcgatcta gcagaggtca tacgatgctt tggagaacct
     3781 gagcaaacat ctcaaaaaat tgaatcatct aagcaagaag gtgatgaatc tacaaaaaaa
     3841 cttattgccc atttagaaaa tgaagtcaaa aaataccaag aacgtgaaga acggttaatg
     3901 caacaaattg accgtatgca aacactcatt gagctgaaaa gtgttgcacc tgccacagca
     3961 gcaccacacc aagatgctac ggcatgcgac accaagatgc cacagcatgc gacaacacaa
     4021 caagacactg ataacaaaaa gaataatgaa ttaaatattg cagaaaatgt agcagtgcca
     4081 cagcaagaaa ctacggcata ccacacccaa acgctacagc atgccacgtt gcaaagtgtg
     4141 gcagtgccac aacacaaaaa acgtggttta tttggccgtg tgctgaatgc cgtctttgat
     4201 aatgactgag caataagggt gaaagatcat gccgaaactg aaagacattg ccctgggaat
     4261 tattgtagct ccgctgctga tcccgatcat gctgattgca tcgtaccagg ataaaaaggc
     4321 gctgaagaaa gagctggatg aatgccaaaa agaaaaagac caggcatcct gattattcaa
     4381 ttgagctaat caaaagtgtg gaacattgat gaaataatag cgtggaacgt gaaaagtcat
     4441 aaaaaaagcc cgaacaggga tgctcgggct ataaactaga aactacagcg ttgaattata
     4501 aggataatta tacagcattt cgtataaggt gtattatgtt aattttagga aatattaaaa
     4561 ctaattttca gacttatccc accaactttg agctaaatca gtatttttag gaatacctat
     4621 accatcacga tacataacgg aaagacttcg cattgctttc ttattaccta actttgcagc
     4681 cttttcacga agttcaaaag ctttattaat attaacttct attccgctat ctgcacgagt
     4741 atatagatca gctaactcaa ccattgattc agaatggcca ttttctgagg ctttctgaaa
     4801 ccacttaaaa gctttttgga aatgttctaa agaatcgttt ttgtcatcat cttccattaa
     4861 tctagcataa gtaacatatc catcagctaa ccaaaacatt gctgctacat ttccattttc
     4921 tgctgattta agagcccaaa cttcagcttg attttcatct gaatcaacac catgaccata
     4981 tagataaatc tcggctaatt ctaactgagc agaagtatct ccattttctg ctttcgctaa
     5041 taaatcatta gaaaaaacag gttctgcaaa tgtttccatt gaaaggcaaa ctcctaaagc
     5101 cataaaaata tttgttaatt tattcaattt ttaaccttta aactaaactg ttaggcttgt
     5161 atatttttat atcataccta aaagaaaact ctaaaattag agtatgtctt atctctattt
     5221 aacataatgg cgattatacg aagtacactt gtatattaat ataaatatca actacttaat
     5281 aaaaatagaa aaatcccaaa atgcccaaaa agccgacttt gaaacaagtc ggctttttta
     5341 tgagcagttt tgatacttct tgccaataat tttgactaaa aattacacga atctggttga
     5401 gtgatacaat tttgtgatac attattgtat atacaaattt gaatgttttt tatggaacag
     5461 tattttgaat gggatgaggc taagaatcga aagaatcaga aaaagcatga tatctctttc
     5521 gaaactgcaa gccttgtttt tgaagatcca ttacggatct caatccaaga cagacatacc
     5581 aatggtgaag aacgttggca aaccattgga cgagttaaag gcgtactaat gttattggtg
     5641 gctcacacca tttttgatga agatgactgt gaaatcatcc gaatcattag tgcaaggcaa
     5701 gtcactaaag cggagcgaaa caaatatgag catggttaga tactcacaca aagagctgaa
     5761 tgagaaattt ggtgagaagc aagatgctga aattcaacgc ttgcttgcta aaggcacggt
     5821 tcctgatgat cagctagatc tatcagatat tcctgaaatt acagactgga gcaatgctgt
     5881 acgccaaaat caattttacc gcccagtaaa gcaacaaact tcaattcggt tagatgctga
     5941 tgtacttgct tggttcaaag cacaaggcaa aggttatcaa acacgtatga atgaaatttt
     6001 acgagatgct atgctaaaag aattaaaaaa tcatcaataa gaatgggagc ttaggctccc
     6061 atttttgact tcatttttag gggggattac cattcaaaaa tttaaatagt agattttata
     6121 aatttttaag tgttatatta taatataaca ttaaatttgg ttgttttatg ctaaataaaa
     6181 gtaaactatt tttagcttta attactttag gggcctcgaa aattttattg gcagctgaag
     6241 ggcctgttac taccttaaat acaatagtat taactgcaca atctgatgag ttagggtctg
     6301 agctgttagg caaaagtcta aatgttagta atcagtttat tgatactagt aaacttaaac
     6361 agcgttcgac aactctaggg gatgccttgg gtacggaatt aggaattcat tctaatcaat
     6421 atggaggtgg agcatctact cctattattc gtgggcagga aggaaaacgt attaaagttt
     6481 tacagaataa tgctgacgtg ttggatatgt ctaatatgtc cccagatcac gctgtcacag
     6541 ttgaaccaag tttagctaag tctattgaaa ttatacgtgg tgcaagtacc cttttgtata
     6601 gttcaaatag tgcggcaggg gtagtcaatg tcattgacta taaaattcca acccaaatgc
     6661 ctcaagacgg attagagggt aatactacat tacgttttaa tactggcagt aatgagaagt
     6721 tgaccacagc aggtgtaaca gttggcttaa gcccacatgt agctttacgg gcagaagggt
     6781 tatatcgtaa tgctggtaac tataaaactc ctcattacca gtctagctct tataattctt
     6841 tagaagatct tgaaaaccaa aacagtattt ataaaaattt aaaatattta ccagaaagct
     6901 gggcagagtc gagagtaggt acgcttggat tatcctggat agacgataat acttatttag
     6961 gagtttctta tacacatcgt catgatgaat atggtttacc agcccatagc catttatatg
     7021 aaggttgtgg tgcctctgca atcggtatcg attcacgaat tagtggatta aaaaattatc
     7081 ttttatatta tcctcaattg atggatgagc aagatattaa ttacataaat ccacgtcctg
     7141 attgtcatca gcataatcat attcatgaaa ctaatttctc tcataatgct ccttatattg
     7201 atttaaatac tcgacgttat gatgtgcgtg gagaattcac tcaaccattt accggtattg
     7261 acaaaattcg tacctcattg agttatatag attattttca caatgaactc gagggagata
     7321 agattactaa cttttttaaa aatacaggaa aagtaggccg catagagtta tcacatcagc
     7381 cattagggga attaaccggt atcttggggc tacaatattt ggaacaggat aatagtgctt
     7441 tatcaccagt ccattctcaa gaaggccata ccacctatct agataatcag cagctattaa
     7501 atcgaaatgt cactaaaaac tttagtgtat ttggcctaga aaaatataac tggaacgact
     7561 ttactttcga actaggtgct cgtatagaaa agcagaaagt tagtatggat tatgatatag
     7621 aaaaaattaa agattcaatg aaaccttggc ctaataaata taacagtcct tatgtagaaa
     7681 aaaataataa aatcagggct caaaatttaa aatctatttt agaagctgtt caacctaaca
     7741 aagaaacagc tttttcttat gcaggtacag tccactggag atttgctcca aattatatct
     7801 tatcattaac aggtacacat caagaaagat tacctaatgc acaagaaatg tatactcatg
     7861 gtatgcatct cgctactaat tcttttgaaa taggtaaccg ctttctaaga aaagagaaat
     7921 ccaataattt agaaatcagt ctggcctata aggatgattt acttgattat caaatcagta
     7981 cttactatta tgattttgat aactatatat atttacagac actcaatgaa gttttaggaa
     8041 ctacaaaagt tagagatcag catacactac gtattaatca ttatagccag tctgctgcta
     8101 atttttatgg attagagggc aacatagggt atcaatttaa ttctgtttat cacggctctc
     8161 tttttggaga ttatgtgaaa ggccgtttaa caaatttacc tgatgctgta attgcatacg
     8221 atatctggaa tagagaaccg actttagctc cccaaaagga tcgttacact cctagattac
     8281 ctcctgctcg tttgggtaca cgactaaaag ctgattttga tgaatcgctt aaaggtgaaa
     8341 tagaatatta tcgagtcttt aaacaagata atatttctaa atttgagcag gttacatcag
     8401 gttataatat gcttaatatg actttagcat ataaaaataa gctatctcac acagaatatg
     8461 atttattctt taaagcgaat aatttacttg atcagaaagt ctatgcccat gaaacatttt
     8521 tgccatatat tccccaaatt ggaagaaatt ttagtttagg cttaaatctt aatttctaga
     8581 taagatactg taatcttcta agaaaatttc ttagaagatt tttcttataa aatgattaga
     8641 ttaataatta aggtaagcgt ccgctgaatc ccatacctat tttttaattc gatttaggtt
     8701 tccagcagtg tggcagtaac tcttcaatct gggtcacttt atgtgtcggc agccttttca
     8761 gca
//
