LOCUS       NZ_CP121565            12723 bp    DNA     circular CON 17-MAY-2024
DEFINITION  Acinetobacter baumannii strain RAB94 plasmid pRAB94-3, complete
            sequence.
ACCESSION   NZ_CP121565
VERSION     NZ_CP121565.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27010289
            Assembly: GCF_029713785.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 12723)
  AUTHORS   Alghorbi,M.F., Bawazeer,R., Okdah,L., Alswaji,A., Alzayer,M.A.,
            Alrashidi,E., Alshahrani,A.S., Al Johani,S.M., NGHA Surveillance
            Group,N.S.G., Balkhy,H. and Doumith,M.
  TITLE     Antimicrobial resistance and genomic characterisation of
            Acinetobacter baumannii clinical isolates from a nation
            representative surveillance in Saudi Arabia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12723)
  AUTHORS   Alghorbi,M.F., Bawazeer,R., Okdah,L., Alswaji,A., Alzayer,M.A.,
            Alrashidi,E., Alshahrani,A.S., Al Johani,S.M., NGHA Surveillance
            Group,N.S.G., Balkhy,H. and Doumith,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-APR-2023) Infectious Diseases Research, King Abdullah
            International Medical Research Center (KAIMRC), KAIMRC, P.O Box
            22490, Riyadh 11426, Saudi Arabia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: EToKi v. 1.2
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 142x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029713785.1-RS_2024_05_17
            Annotation Date                   :: 05/17/2024 05:10:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,916
            CDSs (total)                      :: 3,822
            Genes (coding)                    :: 3,734
            CDSs (with protein)               :: 3,734
            Genes (RNA)                       :: 94
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 27 of 88
            Pseudo Genes (incomplete)         :: 48 of 88
            Pseudo Genes (internal stop)      :: 18 of 88
            Pseudo Genes (multiple problems)  :: 5 of 88
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP121565.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12723
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="RAB94"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pRAB94-3"
                     /geo_loc_name="Saudi Arabia:Riyadh"
                     /lat_lon="24.7136 N 46.6753 E"
                     /collection_date="2019"
                     /collected_by="KAIMRC AMR WGS_Based Surveillance Program"
     gene            7..513
                     /locus_tag="MTS31_RS19505"
                     /old_locus_tag="MTS31_19505"
     CDS             7..513
                     /locus_tag="MTS31_RS19505"
                     /old_locus_tag="MTS31_19505"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019949.1"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="WP_001019957.1"
                     /translation="MNIFIRDEREEDIKEIEELTKAAFLNAEHTSHTEHFIVNSLRKHK
                     QLTVSLVAVEDNTIVGHVAISPVQISSGEKNWYGLGPISVAPNKQGQGIGSLLMNSSLE
                     KLKKSGAKGCVLLGDPKYYSRFGFKAYPDLKLPGVPPEYFQALSFQNYVPKGDVSYHEA
                     FNAQD"
                     /name="N-acetyltransferase"
     gene            complement(546..929)
                     /locus_tag="MTS31_RS19510"
                     /old_locus_tag="MTS31_19510"
     CDS             complement(546..929)
                     /locus_tag="MTS31_RS19510"
                     /old_locus_tag="MTS31_19510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000825559.1"
                     /translation="MKRLIILFLLAYATSSFAQVPFEVSKSCFVVNGRNITEPCLLSST
                     NNSTSNFERLTFANTKVFIKESNICSNNDSCVSVGSNLSNLKDATIYYRDLKTKKIIEK
                     PEKDSWTCFKQPIDKLDFCISYN"
                     /name="hypothetical protein"
     gene            complement(1104..1385)
                     /locus_tag="MTS31_RS19515"
                     /old_locus_tag="MTS31_19515"
     CDS             complement(1104..1385)
                     /locus_tag="MTS31_RS19515"
                     /old_locus_tag="MTS31_19515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000650375.1"
                     /translation="MKCSYQDLDGNIVELTCESYIHIPSGASGNKLGIDNRAEDLIHIT
                     EDFSFFKKTEADSIPLYRFAVDANYNVFNSDELPALAQIGTDWKVLNE"
                     /name="hypothetical protein"
     gene            complement(1574..2044)
                     /locus_tag="MTS31_RS19520"
                     /old_locus_tag="MTS31_19520"
     CDS             complement(1574..2044)
                     /locus_tag="MTS31_RS19520"
                     /old_locus_tag="MTS31_19520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001123845.1"
                     /translation="MPKFLIIAEKVYKKLEEDKLFSDNLIEQLNNLVSIIRKEIKGTPC
                     KLKYNFIDFEECLSKPLSECTIKLDMSLMPKYKNKGEYIMWLASFIERITVGGQPKLPP
                     LSSIVPADFNVKKEAQTIVENKKSVSEENAKMIVNYFNSEEYQKNNKKINLV"
                     /name="hypothetical protein"
     gene            complement(2457..2708)
                     /locus_tag="MTS31_RS19525"
                     /old_locus_tag="MTS31_19525"
     CDS             complement(2457..2708)
                     /locus_tag="MTS31_RS19525"
                     /old_locus_tag="MTS31_19525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002056849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000179474.1"
                     /translation="MATAIENRARQLKNAKRGGYAPTIAKDVNKHKVQKIRRALDEARR
                     YVSELNDETVIFDDQDARQKAEGAKAIIEMFEAALSGA"
                     /name="hypothetical protein"
     gene            complement(3007..4242)
                     /locus_tag="MTS31_RS19530"
                     /old_locus_tag="MTS31_19530"
     CDS             complement(3007..4242)
                     /locus_tag="MTS31_RS19530"
                     /old_locus_tag="MTS31_19530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345330.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="WP_000345328.1"
                     /translation="MDFFNFYRDLQAELSNYPWLEMLVSLTLLILFAALANFIAKRIIV
                     RGIRHLVTKIKSLNQSVFAQHSVIKRFANIVPAIVIMNGITTVPHLSEKAVALVQMGAQ
                     AFIFLTIALTISELLNIFNLIYQRNPKSRNKPIKGYLQLVKLIIFVVCGLMILGTFLKK
                     DVFTLLAGFGAMAAVLMLVFQNTILSLVASVQIASYDMVRIGDWIEMPSLNADGDVIDI
                     SLHTVTVQNFDKTYTTIPTNKLVTDTFKNWRGMSNAGCRRMKRSLYIDQTSVHFMTEEE
                     QQKLKDFLLLDQYLNAKQSEIDEFNKHLGSQSRYNKRRLTNIGTFRAYVEFYLRQHKGI
                     AQNQTLIVRQLQPTSEGLPLEIYAFTNTTAWVSYEAIQSDIFDHLIAILPEFGLRVYQA
                     PSGHDFQKLTVE"
                     /name="mechanosensitive ion channel domain-containing
                     protein"
     gene            4692..6962
                     /locus_tag="MTS31_RS19535"
                     /old_locus_tag="MTS31_19535"
     CDS             4692..6962
                     /locus_tag="MTS31_RS19535"
                     /old_locus_tag="MTS31_19535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004995771.1"
                     /GO_function="GO:0015267 - channel activity [Evidence IEA]"
                     /GO_process="GO:0015891 - siderophore transport [Evidence
                     IEA]; GO:0055085 - transmembrane transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="WP_000527017.1"
                     /translation="MGNKIKSNKLFTRKSEAKNIIPLLSLGGAIFLSNSAFAASPSETT
                     DAEKKPEALPTITINASRADELSTSAKQVTKLDEKQIELLRNGSSGNIATVLAKAVPGL
                     SDSSRTITDYGQTLRGRNALILVDGVPMNLTRDTSRGLSAIDPESIANIEVIRGSNAIY
                     GGGASGGIISITTKAAGSEPTAKTVVGLQTPLTNFRSNALSGDIHQYFTGSFNAFDYAL
                     DFGYQRIGSPYDASGDRVAPEPSQGDLYDSDGYSIGGKLGYHIDDNQYVQFAANYYNAE
                     QDSDYASDPSVKNAPAGTVPAKAIKGLKLKDQNKNENQIYNLTYNHKDFFGNKVDAQIY
                     YRDFFTRFSPFDARKNPNRGGNVDQIYQENNILGSRLTVTTPLEFLGDTSLVWGGDFSR
                     EKSEMPLDTFNPDIYDQSGGLDFVKTGKLIYLPELTTQSVGGFVQLKHRFNDQWSAEAG
                     TRYEDSYAQIDSFIPLSQFDPKTQSYKPNPYVYQGGKVKADAWLYNANVTFSPNDQHSI
                     YASFNQGFQLPDVGVIIRNADNTDKEKNYELSSSFLEPVKVDNYELGWKGNFNNFSSSL
                     AVFHSTSDLGAVQSFSNGLALTRTKEKVTGLEATFDYLDDANVWGTGGSVTWMKGREKP
                     QNGAEQDMTGFRIPPLKLTGYISYSPTETWTNRLQATYFGSEDYRLNGKNSFGRYDVKS
                     YTTADLISSFALNKKDTMTIGLENMFNRKYYPLYSQLLRTSDNTSHLVANGVTLKVTYS
                     HKW"
                     /name="TonB-dependent receptor"
     gene            complement(7025..7579)
                     /locus_tag="MTS31_RS19540"
                     /old_locus_tag="MTS31_19540"
     CDS             complement(7025..7579)
                     /locus_tag="MTS31_RS19540"
                     /old_locus_tag="MTS31_19540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016653936.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_001133110.1"
                     /translation="MPNLEASFRALRVLHTARDLFKQYGFHKVGVDRIIAESKITKATF
                     YNYFHSKERLIEMCLTFQKDGLKEEVFSIIYSYRELMVFDKLKKIFFLHANLEGLYRLP
                     LQAIFEIEKFYPTAYKVVVDYRNWLVIQIHQLLLTIKATATLEDAYMFLFVIDGAMVQL
                     LSKDRIDERDKLLDYFLIMMS"
                     /name="TetR/AcrR family transcriptional regulator"
     gene            complement(8519..8857)
                     /locus_tag="MTS31_RS19550"
                     /old_locus_tag="MTS31_19550"
     CDS             complement(8519..8857)
                     /locus_tag="MTS31_RS19550"
                     /old_locus_tag="MTS31_19550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004994769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001243511.1"
                     /translation="MRNFLVTIFTLLVGTSIYASQESRNLLEQTQCEKAVELHGFLSRA
                     QLDCNYHYYSEELKEAAAKCTKHDLGEKYGREVMKFGMKEFEERKKEDTQGHFCHKVLK
                     EFPKYIKQ"
                     /name="hypothetical protein"
     gene            9467..9952
                     /locus_tag="MTS31_RS19555"
                     /old_locus_tag="MTS31_19555"
     CDS             9467..9952
                     /locus_tag="MTS31_RS19555"
                     /old_locus_tag="MTS31_19555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004938816.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="WP_001089196.1"
                     /translation="MNSTEYALDRIDRKILSALRRNGRLTVAQLAEEVGLSSSPCWTRL
                     KRLESLKIIEGYTVNVNPKAIGIHELFFIEITLERHDDEMLENFSEALADIPEVVEAHL
                     VTGDYDYLVKVAVKDAEHYERFLRKKLYSIKGIRHTRSTFALRPLKSANTADLMLIE"
                     /name="Lrp/AsnC family transcriptional regulator"
     gene            complement(9981..10538)
                     /locus_tag="MTS31_RS19560"
                     /old_locus_tag="MTS31_19560"
     CDS             complement(9981..10538)
                     /locus_tag="MTS31_RS19560"
                     /old_locus_tag="MTS31_19560"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932078.1"
                     /GO_process="GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="WP_000932097.1"
                     /translation="MLNIKNIQQTVKVATTVAALMCFSSLAQAYQYEQTARLINERLSY
                     MKDVAGYKAEQHLPIEDLTQEKKVLDQSLSEADSFGLNSETVKPFIVVQMDVAKAIQYR
                     YRADWLSSPETNWKPQDLSEVRLKISALNTELLKNIAYELKKNNNKAPHGCSYMWPVQH
                     PQLKEADKRALCVTLNKIKLKQ"
                     /name="chorismate mutase"
     gene            complement(10616..11299)
                     /locus_tag="MTS31_RS19565"
                     /old_locus_tag="MTS31_19565"
     CDS             complement(10616..11299)
                     /locus_tag="MTS31_RS19565"
                     /old_locus_tag="MTS31_19565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004995838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_000920697.1"
                     /translation="MLKTIFQKFQKKQGCVVIAGHDIELLDQNFVILDKNEQSPLHVYQ
                     AVHDSSVQNIQVNRGNDGITSVRLNLVNTEHLKSLLKYIASHSHNIELCVFQPNFSSAP
                     NIEALSLEEIEHSWQTTGLSAVSVSQAVIKPMLKQQRGTVIFLGAPSNNSAHYDVLSQS
                     MFASIRALSQSLAREFQPKGIHVTYCMVEKWDGQNQHFISSVKQVCQHIYQQPNTAWSQ
                     ELSVS"
                     /name="SDR family NAD(P)-dependent oxidoreductase"
     gene            complement(11309..>11545)
                     /locus_tag="MTS31_RS19570"
                     /old_locus_tag="MTS31_19570"
                     /pseudo=""
     CDS             complement(11309..>11545)
                     /locus_tag="MTS31_RS19570"
                     /old_locus_tag="MTS31_19570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004995839.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /name="short-chain dehydrogenase"
     gene            complement(11563..12653)
                     /locus_tag="MTS31_RS19575"
                     /old_locus_tag="MTS31_19575"
     CDS             complement(join(11563..12113,12113..12653))
                     /locus_tag="MTS31_RS19575"
                     /old_locus_tag="MTS31_19575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612069.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISAba1 family transposase"
                     /protein_id="WP_085940413.1"
                     /translation="MTHLNELYLILNKYLKWNKSHLKCFALIMLVIILKQTCNLSSASK
                     ALPIKCLPQSFYRRMQRFFAGQYFDYRQISQLIFNMFSFDQVQLTLDRTNWKWGKRNIN
                     ILMLAIVYRGIAIPILWTLLNKRGNSDTKERIALIQRFIAIFGKDRIVNVFADREFIGE
                     QWFTWLIEQDINFCIRVKKNFIVTNHLGKNHKISDLFRHLKVGQIECRKRRILVGRVKL
                     YISALQLENGELLLVVSPQFNANAIQDYALRWEIETLFSCLKGRGFNLENTRLTDPRRV
                     KKLIAVLAISFCWCYLTGEWQHDQKKAIKIKKHGRLSMSLFRYGLDYVQMAIQRLIGFG
                     KKEEFKEILAILRRQNPDRIRVL"
                     /name="IS4-like element ISAba1 family transposase"
ORIGIN
        1 cttaagatga atatttttat tcgcgatgag cgagaagaag atattaaaga aattgaagaa
       61 ttgactaaag cggcatttct aaatgctgaa catacgagcc atactgaaca ctttattgtt
      121 aatagtttaa gaaagcacaa acaacttaca gtctctttag ttgcggttga agataatact
      181 attgttgggc atgtggcaat ttcaccagtg caaatttcat caggtgaaaa gaattggtat
      241 ggcttaggac ctatttctgt cgcgccgaat aagcaaggac aaggcattgg atcattgctt
      301 atgaatagct ctttagaaaa attaaaaaaa tcaggcgcaa aaggttgtgt tttattaggt
      361 gatccaaagt attacagtcg ctttggtttt aaggcatacc ctgacctaaa actacctgga
      421 gtgccccctg aatacttcca agccttaagt tttcaaaatt atgttcctaa aggtgatgta
      481 agctaccatg aagcatttaa tgcccaagac taatttagaa ttttctttat taaaattaga
      541 ttttattaat tataagaaat acaaaagtct aatttatcaa ttggttgctt aaaacaggtc
      601 catgaatctt tttccggttt ttcaataatt tttttagtct ttaaatcacg ataataaata
      661 gtcgcatctt ttaaattcga caaattactt cctacagaaa cacatgaatc gttatttgaa
      721 caaatatttg attctttaat aaaaactttt gtattagcaa aagttaatct ttcaaagtta
      781 gatgtcgagt tatttgtaga agaaagtaag caaggctcag taatatttct accattcact
      841 acaaaacaac ttttactgac ttcgaatggc acttgagcaa aggagcttgt agcataagct
      901 aatagaaata atataataag gcgcttcatg ttctttctct ctatataatc aacatctagt
      961 ctaaaactgc ctataaatat ttagactata ttttcagttc gctatttata atacctgtat
     1021 taagataagt taaatattta ctattcgata aattttacaa aatattaaag ctctagctta
     1081 taggctacct aacaatcgca aagttattca ttaagcactt tccaatcggt tccaatttgg
     1141 gccaaagccg gtagctcatc actattaaaa acattgtaat tagcatctac agcgaatcga
     1201 tagagtggta ttgaatctgc ctctgttttc ttaaaaaaac taaaatcttc agtgatatgt
     1261 attaagtctt ctgccctatt atctattccc aatttatttc cactggcacc tgacggtata
     1321 tgtatataac tttcacatgt taattcgact atatttccat ctagatcttg atatgaacat
     1381 ttcatgttta aacccttccg taatttttat agatgtgtag aaaaacttaa catgctatac
     1441 agataagtta tggaattttt tatgatgtaa ttgtttaagt atgtatatgg caaaagaaaa
     1501 ccgccccaga aggcggttgc tgaaattttc cagcgattat aaaagtgcgg tggacacaca
     1561 cttttatatg gttttaaacc agatttattt ttttattatt tttctgatat tcttcagaat
     1621 taaaataatt cacaatcatc tttgcatttt cttccgatac tgatttttta ttttcaacta
     1681 tcgtctgagc ttcttttttt acgttaaagt cggctggcac aatacttgaa agtggtggta
     1741 attttggctg cccgccaacc gtgattcttt caataaagct cgctagccac ataatatatt
     1801 cacccttatt cttatattta ggcattaaac tcatgtcgag tttaatcgtg cattcactta
     1861 agggtttact taggcattct tcaaaatcga taaaattgta tttgagttta cagggagttc
     1921 cctttatctc tttacgaata atactgacca agttatttag ttgttcgatc aggttatctg
     1981 aaaacaattt atcttcttct agttttttat aaaccttctc ggcaattatt agaaattttg
     2041 gcatatttcc ttccctctta tattgaccat cgcgattgat cagactttta gggaagtatg
     2101 atgaatatat tattgcaata tgtaagtgtt gaaataccaa atgtaatcta taagagtgtt
     2161 ctgaaactga cttccataac gaaccacagc aatgaataaa aatgtaaaat ccatcacatt
     2221 ttaactccga aaaattttta tagttagtaa taattttcta aaaaaattaa gctcatcaat
     2281 aatattattt gaccggtatt tttttggttt ttttgagtaa aaaatagaat atagaaatat
     2341 ttgaattatt tttattttac tgccttgctg aataaccaag ttgcctagtc aaggggctag
     2401 aacgttaacc gtgtttcagc aaggtctttt atgattatgt tttagtttga tgctgctcac
     2461 gcaccgctta aagctgcttc aaacatttca ataattgctt tggctccttc tgccttttgc
     2521 cttgcgtcct gatcgtcaaa gataaccgtt tcgtcattta gttcactcac atatcgccgg
     2581 gcttcgtcta gagctctcct tattttttgt actttatgtt tgtttacgtc tttggcaatg
     2641 gttggcgcat atcctccacg tttagcgttt ttaagttgtc tagctctgtt ctcaattgct
     2701 gtagccattc aaacactcca actcacctaa caaatcttag taaagcaact ttattgtttg
     2761 ttatttttaa acatttagta ttttctttgt tcaaagtgtc aaatttgtta cttaagcctt
     2821 actcctgcaa gttttagatt gagtttgctt aaaatctttt aagctacaat gtgcatgtag
     2881 ctttcaaagt caacttatac agctatttaa taataatttt ctaagaatag ttataaatta
     2941 atgtataaaa ttattaaaaa gatgctatct atttgaaaat aaaatagata gcacgctaac
     3001 agttgtttat tctactgtta atttctgaaa atcatggcct gaaggtgcct gatacactct
     3061 taagccaaac tcaggcaaaa tcgcaatcag atgatcaaaa atatcggact gaattgcttc
     3121 ataagaaacc catgcagtgg tattggtaaa tgcatagatt tctaatggta gaccttcact
     3181 cgttggttgt agttggcgaa caataagcgt ttgattttga gcaatcccct tatgctggcg
     3241 caaatagaat tcaacatatg ctctaaatgt tccaatattg gttaagcgtc ttttgttata
     3301 acgtgactga ctacctaaat gcttattaaa ttcgtcaatc tcagattgct tggcattgag
     3361 gtattgatct agcaataaaa aatctttgag tttttgttgc tcttcttcgg tcatgaagtg
     3421 cacgctagtt tgatcgatat aaagagaacg cttcatacgg cgacagcccg cattactcat
     3481 accacgccag tttttaaaag tgtctgtcac tagtttattg gttggaatcg tggtgtatgt
     3541 tttatcaaag ttttgaacgg tcaccgtgtg caatgaaata tcaatgacat caccatcagc
     3601 attgagcgat ggcatttcaa tccagtcccc aatacgtacc atgtcataag aggcaatttg
     3661 tacactcgcg accaatgaca aaatcgtatt ttggaaaact aacattaata cggctgccat
     3721 tgcaccgaag ccagccaaca acgtaaatac atcctttttc aggaatgtac ccaaaatcat
     3781 taatccgcaa acaacaaaaa taatgagttt aacgagttgc aaataacctt taatcggctt
     3841 gtttctagat tttggattac gctgatagat taaattaaat atatttaata attcactaat
     3901 ggtgagcgca atcgtgagga aaatgaatgc ttgtgcgccc atctgtacca aagcaaccgc
     3961 tttttccgac aaatgcggaa cggtagtaat accattcata ataacaatgg ccggcacaat
     4021 attggcaaat cgcttaatga cactatgctg ggcaaaaact gactggttga gtgacttaat
     4081 tttagtcact aaatgacgta ttccccgaac aataattctt ttggcaatga aatttgctaa
     4141 tgcagcaaaa aggatcaaaa gtgtgagcga aactagcatt tctagccaag gatagttgct
     4201 taactcagct tgaagatcac gataaaaatt aaaaaaatcc aaaacctaca ccttaaaact
     4261 ttattttcaa taattttaaa gtgttcatgt gttatagatt aagacttgtt tacatatgtc
     4321 gtcataaata caacaaggaa gtaatattca atcgtaaatt tagataaaac ctgatcacta
     4381 ctattcgact agacaataaa tagattaata agtatggaat aggtggaaaa atatttatct
     4441 caagtcactg cttctctttg tacctatata aaaaaagatt ttgactcctt aagaatgctg
     4501 catatgaaaa agtttaaggc ttttcaaata tattaaatta atcaaaactc ttaactctct
     4561 tagaagatct ttgttattta tgtttattta agagccacaa atacttctat tatatagatt
     4621 aataatatta ttaattaaaa tcattctcat ttataattcg acgaatttac aatcactctc
     4681 agggcgagaa aatgggaaat aagataaaaa gtaataaact ttttactagg aagtctgaag
     4741 caaaaaatat cattccactt ctatcacttg gtggagccat ctttctctca aactcggcct
     4801 ttgcagcctc gccttctgag acaacagatg ctgaaaaaaa gcctgaagca ctccctacaa
     4861 ttacaatcaa cgcttcacgt gcagatgaac tttctacatc tgccaaacaa gtgaccaagc
     4921 tagatgaaaa acaaattgaa cttttaagaa atggttcatc tggaaatatt gcaactgttt
     4981 tagctaaagc cgtaccgggc ctttccgact caagccgaac cattaccgat tatggtcaaa
     5041 ctttacgggg ccgtaatgcc ctcatcttgg tcgatggtgt tccaatgaac ctgacacgtg
     5101 acacatcaag aggtctttca gctatcgacc ctgaaagtat tgctaacatt gaagtcattc
     5161 gcggtagtaa cgcaatttat ggcggtggcg cttctggcgg tattatttca attaccacaa
     5221 aagcggctgg tagcgaacct acagctaaaa cagtcgttgg acttcaaaca cctttaacaa
     5281 atttccgttc taatgcttta agtggcgata ttcatcaata tttcacaggc agctttaacg
     5341 cttttgacta tgcactcgac tttggttatc agcgtattgg tagcccgtac gatgcaagtg
     5401 gcgaccgtgt tgctccagaa ccaagtcagg gcgatttata tgattctgac ggctatagca
     5461 tcggcggtaa gcttggttat catattgatg acaaccaata tgtccagttt gctgcaaact
     5521 actataatgc cgagcaagat tcagattatg catctgaccc aagtgtgaag aatgcccctg
     5581 ccggaaccgt accagccaaa gccattaaag gcttaaagct caaagaccaa aataaaaacg
     5641 aaaaccagat ctacaattta acctataacc ataaagactt ttttggcaat aaggttgatg
     5701 ctcaaattta ttaccgcgac ttctttaccc gtttttcacc atttgatgca cgtaaaaatc
     5761 cgaatcgggg tggaaacgta gaccagatct atcaagaaaa taacatactc ggtagccgtt
     5821 taactgttac cacaccgctt gagtttttag gtgacacctc actggtttgg ggtggtgact
     5881 ttagccgtga aaaaagcgaa atgcctttag atactttcaa tcctgatatt tatgaccaat
     5941 ctggtggcct agattttgta aaaactggta agctcattta tttacctgaa cttaccacac
     6001 aaagtgttgg tggctttgta cagctaaaac atcgctttaa tgaccaatgg tcggctgaag
     6061 caggtacacg ctatgaagat agctatgccc aaattgatag ttttattcca ttatcacaat
     6121 ttgatcctaa gacacaatca tataagccaa acccttatgt atatcaaggt ggaaaagtaa
     6181 aagctgatgc atggttgtac aatgctaatg taactttctc tccaaatgac cagcactcga
     6241 tttatgcatc gtttaaccaa ggttttcaat tacccgatgt tggtgtaatt attcgtaatg
     6301 cagataatac tgataaggaa aaaaattatg aattatcatc ttcattttta gaacctgtaa
     6361 aagttgataa ctatgagttg ggctggaaag ggaacttcaa taacttttca tcaagcttag
     6421 cagtcttcca ctctacttct gatctaggcg ctgtgcagtc tttcagcaat ggtctggcac
     6481 ttactagaac caaagagaaa gtcactggtc ttgaagcaac ttttgactat cttgacgatg
     6541 ccaatgtttg gggaactggt ggtagcgtta cgtggatgaa aggccgtgaa aaaccgcaaa
     6601 atggtgctga gcaagacatg acaggcttcc gtattccgcc attaaaactc acaggttata
     6661 tttcttatag cccaacagaa acttggacaa accgtttaca agccacttac ttcggttcag
     6721 aagattaccg cctaaacggc aagaatagtt ttggtcgcta cgatgtaaaa tcttatacaa
     6781 cagcagattt aatcagtagc tttgcactta ataaaaaaga caccatgacg attggtttag
     6841 agaatatgtt taaccgcaag tactatcctc tatatagcca attattaaga acaagcgata
     6901 ataccagtca cttagttgca aatggtgtga cgttaaaagt gacttatagc cataagtggt
     6961 aattgagatt ttagttagta actaacatta taaaagcccc attatcgggg cttttatttt
     7021 tagtctagga catcattatt aaaaaataat ctaataattt atctcgttca tctattctat
     7081 ctttactcaa aagctggacc attgccccat caatcacaaa caaaaacata taggcatctt
     7141 cgagtgtagc tgtggcttta atggttaaaa gtaattgatg tatttggatc accaaccagt
     7201 ttctataatc aaccaccacc ttgtaggcag ttggataaaa cttttcgatt tcaaaaatgg
     7261 cctgtaaagg taaacggtac agcccttcga ggttggcatg cagaaaaaag attttcttga
     7321 gcttatcaaa taccataagt tcacggtatg aataaataat agaaaatact tcttctttta
     7381 gcccgtcctt ttgaaaggtt aagcacattt caatgagtct ttctttagag tggaaatagt
     7441 tgtaaaaggt ggctttggta attttagact cagcaataat tcggtcgaca ccaaccttgt
     7501 gaaatccgta ttgtttaaat aaatctcttg ctgtgtgaag cactctaagt gctctaaatg
     7561 aagcttctaa atttggcata gttataccgc ttaaaataat tttttgttgt atataaaaga
     7621 gatagacatg ccgtcactct taaattgagc gctaaaaaca gacatgcaaa agccgcacaa
     7681 taaagatgtg ctcgcctatc tcgggtttat tgttgctatg atttttgagt cgtaacgatt
     7741 tttaaggtgc aaaaacctaa agtcgattca accgctttat agactgttta agtggtatag
     7801 acagaaagac tttggcaaaa accaataaat gagaagaatt agtacgcata gcgaactcct
     7861 ttggctataa agaagtttca ccacattact gctaaataat ggtggcgaaa cgaaagaggg
     7921 ttagcagact ggagccaaag aatccagcac acccgaaggt gtccccctcc cgttccgccg
     7981 cagagggggc acgcagggat tcccacaaaa aagtgattac tttttcgtgt tctttggcta
     8041 atggtctgct aaaaccaact ggcaatgtag gccagcaggc aaaggataat cggaggggtt
     8101 ttaggtgtca atgtgatatt ggctagaaat aaagctttaa aaactctata aaataacttt
     8161 acttaagatt tagtcatcca ttctagaatc ttttattttt gaaatttaaa aatataagta
     8221 aacttaaaat gtcctccgct atattaatta agactttgaa tgccgactta tgcttatttt
     8281 tcctccataa tacataacga acggctaatc caaggaagga aatgttattt ttatggttac
     8341 aataagggat tcaaactaaa aaatgattgt tgttagatct aaaacaaggt gaagttttat
     8401 attagctaat tttttaagaa ttattttcta atttaactag taaatttata gaccgataaa
     8461 atgaataatt aaatccatct tatcggcttg ctagaagagg tttttaatac cttatttttt
     8521 attgtttaat atattttgga aattctttta aaaccttatg acaaaagtgg ccttgtgtat
     8581 cctctttctt ccgctcttca aattccttca tcccaaattt cataacctct ctaccatatt
     8641 tttcgccgag atcatgttta gtacattttg cggcagcctc ttttaactct tctgaataat
     8701 agtgatagtt acagtcaagt tgagctcggc ttaaaaaacc atgaagctct actgcttttt
     8761 cgcattgtgt ttgctctaga agatttctag attcttgact cgcataaatt gatgttccta
     8821 ctaataaagt gaaaatcgtt actaaaaaat tacgcattat ttccccgaca aagttattaa
     8881 aagtattagt cataatattg cgggcgtatt aaataataat tatcactaag tgtctattaa
     8941 taataaagaa aataggataa ccttcttctc tttcgaatat ttaatcacct tattaaaatt
     9001 aggtgattaa gttttaacaa ataaataatc tttattttac ttccctattt aattgttaaa
     9061 ttagtacttt ggttagggat taaagtatgt gcttttataa atttcaataa taatatttag
     9121 ccatttagga tatttaataa gatttttgga aagatgagcg ctatgaccaa taagaatata
     9181 tttatgatta ttattgaacc aatatttgat gattctaaag tatgaatcaa aaattcctaa
     9241 tatttgattt ttgggtaaaa tccgcaaaga gcactgttta gtcaccctta ctgaaacagt
     9301 gctctttgct tcatcacaac tcctatccag tagagtccga tgcatagaaa tagtgtatgg
     9361 ctgtgtcgct caaaatgttt actgaatttt cagttttttt aaatgatatc agcataaaaa
     9421 tcacgtatat ttaaaattaa cagaagaatc ttctttggtt tatattttga acagcacaga
     9481 atacgcacta gatcgcatag atcggaaaat attatctgcc ctacgtagaa atggccgttt
     9541 aacggttgca cagctcgccg aagaagtcgg gctttcatct tcgccttgct ggactcgact
     9601 caaacgttta gaaagtctaa aaattattga aggctatacc gtaaacgtaa accccaaagc
     9661 gattggcatt catgagttgt tttttattga aattacccta gaacgccacg atgatgaaat
     9721 gttagaaaac tttagcgagg ctttggcaga cattccagaa gtggtcgaag cgcatttagt
     9781 tacgggcgac tatgattatt tggtgaaagt ggccgtaaaa gatgccgagc attacgaacg
     9841 ttttttacgt aaaaagctct actcgattaa aggaatccgc cacacgcgtt caacctttgc
     9901 tttacgacca cttaaatctg ccaatactgc cgatttaatg ttgattgaat aatttactta
     9961 tagggagggc tttgggtggt tcattgtttt aatttgattt tattcaatgt tacacataaa
    10021 gccctcttgt cagcttcttt aagttgagga tgctgaacag gccacatata gctacagcca
    10081 tgaggcgctt tgttattatt tttcttaagt tcataggcga tattttttag cagctctgtg
    10141 tttaaagcgc taatttttaa gcgtacttca cttaagtctt gaggcttcca gttggtttcc
    10201 ggactcgaca accagtcggc gcgataacgg tactggattg ctttagccac atccatttgc
    10261 accactataa atggctttac tgtttcgctg ttaagcccaa aagagtcagc ttcagaaagc
    10321 gattgatcga gtactttctt ttcttgtgtt aaatcttcaa tcggtaaatg ctgctctgcc
    10381 ttatagcctg caacatcttt catataagat aaacgttcgt taattaagcg cgcggtttgc
    10441 tcatattgat atgcttgtgc cagtgaagaa aagcacatca aagctgccac tgtagtcgct
    10501 actttaaccg tttgttgaat attcttaata tttaacattt aagtaccttc aaaattattg
    10561 ttctgttgtc attccaaagc agtactctat tttaagaaca ctcgaaagta aaatctcaag
    10621 acacgctaag ctcttgactc catgcagtgt taggttgttg atagatgtgc tgacagactt
    10681 gtttaacaga tgaaataaag tgctggtttt gcccatccca tttttccacc atgcaatatg
    10741 tcacgtgtat tcctttaggc tgaaactctc ttgccaatga ctgtgataac gctcgaatac
    10801 tcgcaaacat gctttggctt aacacatcgt agtgagccga gttattagat ggcgctccaa
    10861 gaaaaataac agtgcctcgc tgctgcttta gcattggttt aatcactgct tgtgacacgc
    10921 tcactgcgct taaacccgtt gtttgccaac tgtgctcaat ttcttctaaa gataaagctt
    10981 caatatttgg cgcagatgaa aaatttggct gaaacacgca taactcaatg ttatgactat
    11041 ggctggcaat atattttagt aagcttttta aatgctcagt attcaccaag tttaaacgga
    11101 cactcgttat gccatcattt ccacggttta cttgtatatt ctgaacgcta gaatcatgca
    11161 cagcttgata gacatgcaat ggagattgct catttttgtc taaaattaca aagttctggt
    11221 caagtagttc tatatcatga cctgcaataa ctacacagcc ctgctttttt tgaaacttct
    11281 gaaatattgt ttttagcata tgagtctctc aaaatttttc ttggtatggg cgtaaatcta
    11341 gctcgtgtgt ccaaagctca gggctttgct ggtagagcat ccaatagttg tcggctatgg
    11401 cattaatatt gagtccaccc gttccccgtg tgagtcgaat gaaacgtcct agaccaaata
    11461 aagctttatt aattctgtcg ccatccacca ttccatcaat aatgacatga gcaatatgaa
    11521 tgccttgagt tttataaatt tgagctctgt acacgacaaa tttcacagaa cccttatcct
    11581 atcagggttc tgccttctta aaattgccaa aatttcctta aactcttctt ttttcccaaa
    11641 accaattaaa cgctgaatcg ccatttgaac atagtctaaa ccatagcgaa ataaactcat
    11701 tgagagtcgt ccatgcttct ttatttttat cgcttttttt tgatcatgtt gccattcacc
    11761 cgttaagtaa caccaacaga agcttatagc taacaccgca atcaattttt tcactcgtct
    11821 agggtctgtc aagcgcgtat tttcaagatt aaacccgcgt cctttgagac aactgaataa
    11881 ggtttcaatt tcccagcgta atgcataatc ctgaatagca ttggcattaa actgaggaga
    11941 aacgacgagt aaaagctctc cattttctaa ctgtagtgca cttatatata gtttcacccg
    12001 accaaccaaa atccgtcgtt tacgacattc aatttgacca actttaagat ggcgaaataa
    12061 atcactaatt ttatgattct ttcctaaatg attggtgaca atgaagtttt tttaacacga
    12121 atgcagaagt tgatgtcttg ttcaattaac catgtaaacc actgctcacc gataaactct
    12181 ctgtctgcga acacattcac aatacggtct ttaccaaaaa tggctataaa gcgttgaatc
    12241 aaagcaatac gctctttcgt atctgaattt ccacgtttat taagcaatgt ccaaaggata
    12301 ggtatcgcta ttccacgata aacgattgcg agcatcagga tattaatatt tcgttttccc
    12361 catttccaat tggttctatc taaagtcagt tgcacttggt cgaatgaaaa catattgaaa
    12421 atcaactgag aaatttgacg ataatcaaaa tactgacctg caaagaagcg ctgcatacgt
    12481 cgataaaatg attgtggtaa gcacttgatg ggcaaggctt tagatgcaga agaaagatta
    12541 catgtttgct ttaaaataat cacaagcatg atgagcgcaa agcactttaa atgtgacttg
    12601 ttccatttta gatatttgtt taagataaga tataactcat tgagatgtgt catagtattc
    12661 gtcgttagaa aacaattatt atgacattat ttcaatgagt tatctatttt tatcgtgtac
    12721 aga
//
