LOCUS       NZ_CP121562             9763 bp    DNA     circular CON 17-MAY-2024
DEFINITION  Acinetobacter baumannii strain RAB97 plasmid pI_RAB97, complete
            sequence.
ACCESSION   NZ_CP121562
VERSION     NZ_CP121562.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27010291
            Assembly: GCF_029713845.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 9763)
  AUTHORS   Alghorbi,M.F., Bawazeer,R., Okdah,L., Alswaji,A., Alzayer,M.A.,
            Alrashidi,E., Alshahrani,A.S., Al Johani,S.M., NGHA Surveillance
            Group,N.S.G., Balkhy,H. and Doumith,M.
  TITLE     Antimicrobial resistance and genomic characterisation of
            Acinetobacter baumannii clinical isolates from a nation
            representative surveillance in Saudi Arabia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9763)
  AUTHORS   Alghorbi,M.F., Bawazeer,R., Okdah,L., Alswaji,A., Alzayer,M.A.,
            Alrashidi,E., Alshahrani,A.S., Al Johani,S.M., NGHA Surveillance
            Group,N.S.G., Balkhy,H. and Doumith,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-APR-2023) Infectious Diseases Research, King Abdullah
            International Medical Research Center (KAIMRC), KAIMRC, P.O Box
            22490, Riyadh 11426, Saudi Arabia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: EToKi v. 1.2
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 462x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029713845.1-RS_2024_05_17
            Annotation Date                   :: 05/17/2024 05:07:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,019
            CDSs (total)                      :: 3,923
            Genes (coding)                    :: 3,777
            CDSs (with protein)               :: 3,777
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 146
            CDSs (without protein)            :: 146
            Pseudo Genes (ambiguous residues) :: 0 of 146
            Pseudo Genes (frameshifted)       :: 50 of 146
            Pseudo Genes (incomplete)         :: 90 of 146
            Pseudo Genes (internal stop)      :: 17 of 146
            Pseudo Genes (multiple problems)  :: 11 of 146
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP121562.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9763
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="RAB97"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pI_RAB97"
                     /geo_loc_name="Saudi Arabia:Riyadh"
                     /lat_lon="24.7136 N 46.6753 E"
                     /collection_date="2019"
                     /collected_by="KAIMRC AMR WGS_Based Surveillance Program"
     gene            complement(126..644)
                     /locus_tag="MTS88_RS20070"
                     /old_locus_tag="MTS88_20070"
     CDS             complement(126..644)
                     /locus_tag="MTS88_RS20070"
                     /old_locus_tag="MTS88_20070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447193.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_000447193.1"
                     /translation="METFAEPVFSNDLLAKAENGDTSAQLELAEIYLYGHGVDSDENQA
                     EVWALKSAENGNVAAMFWLADGYVTYARLMEDDDKNDSLEHFQKAFKWFQKASENGHSE
                     SMVELADLYTRADSGIEVNINKAFELREKAAKLGNKKAMRSLSVMYRDGIGIPKNTDLA
                     QSWWDKSEN"
                     /name="tetratricopeptide repeat protein"
     gene            1017..1304
                     /locus_tag="MTS88_RS20075"
                     /old_locus_tag="MTS88_20075"
     CDS             1017..1304
                     /locus_tag="MTS88_RS20075"
                     /old_locus_tag="MTS88_20075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438826.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            1291..1605
                     /locus_tag="MTS88_RS20080"
                     /old_locus_tag="MTS88_20080"
     CDS             1291..1605
                     /locus_tag="MTS88_RS20080"
                     /old_locus_tag="MTS88_20080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            1733..>2437
                     /gene="znuD2"
                     /locus_tag="MTS88_RS20085"
                     /old_locus_tag="MTS88_20085"
                     /pseudo=""
     CDS             1733..>2437
                     /gene="znuD2"
                     /locus_tag="MTS88_RS20085"
                     /old_locus_tag="MTS88_20085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /name="znuD2"
     gene            2463..3392
                     /locus_tag="MTS88_RS20090"
                     /old_locus_tag="MTS88_20090"
     CDS             2463..3392
                     /locus_tag="MTS88_RS20090"
                     /old_locus_tag="MTS88_20090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091151.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISAba10 family transposase"
                     /protein_id="WP_001091151.1"
                     /translation="MNTESKSRYKTTNWSEYNQALRQRGAFTIWFDPQMQWSATPTGKK
                     GRQPTYTDIAIQFALTIRNLFQLALRQTQGFIQSFLQMAHLDWNVPDFSTLSRRADKLQ
                     PLLHKSAKNPQEDLHILVDSTGIKVTGDGEWVRKKHGVNQHRQWLKLHLATDANSGEIQ
                     AVEVTTCQYGDAEMLQPLLDQIDEPIASVTADGAYDTVNCYDAILKRQANVIIPPRSNA
                     ALWAENEIGKARNHAVQGCWDKGQKQWKRDIGYHRRSRIEAKMFALKRLGQGVSSRCFN
                     RQVVDLQIRVDILNKFTQLGTAKTVAVA"
                     /name="IS5-like element ISAba10 family transposase"
     gene            <3446..5176
                     /gene="znuD2"
                     /locus_tag="MTS88_RS20095"
                     /old_locus_tag="MTS88_20095"
                     /pseudo=""
     CDS             <3446..5176
                     /gene="znuD2"
                     /locus_tag="MTS88_RS20095"
                     /old_locus_tag="MTS88_20095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /name="znuD2"
     gene            5481..5942
                     /locus_tag="MTS88_RS20100"
                     /old_locus_tag="MTS88_20100"
     CDS             5481..5942
                     /locus_tag="MTS88_RS20100"
                     /old_locus_tag="MTS88_20100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(6265..6438)
                     /locus_tag="MTS88_RS20105"
                     /old_locus_tag="MTS88_20105"
     CDS             complement(6265..6438)
                     /locus_tag="MTS88_RS20105"
                     /old_locus_tag="MTS88_20105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001282484.1"
                     /translation="MSDNIIQLSKPCTLCENRQNVQLFAGLMLCGSCQENIRLTNPSMF
                     SANDEIEQKAQN"
                     /name="hypothetical protein"
     gene            complement(6438..6809)
                     /locus_tag="MTS88_RS20110"
                     /old_locus_tag="MTS88_20110"
     CDS             complement(6438..6809)
                     /locus_tag="MTS88_RS20110"
                     /old_locus_tag="MTS88_20110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000504218.1"
                     /translation="MGEKVKTYAVRLDPQVAEFYDQLANSEGIRTSKLFSKILTNDFQS
                     IAVKQQADRIEDLVNRLEKRIDDFEEDNNFSEKYYEDFSGLYFMMLWLLMKNGASKDEV
                     RMIQAKGYSYADDNYKRVK"
                     /name="hypothetical protein"
     gene            complement(7081..7335)
                     /locus_tag="MTS88_RS20155"
     CDS             complement(7081..7335)
                     /locus_tag="MTS88_RS20155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001014303.1"
                     /translation="MNGQKIDFLRFSKQGNKYFFPCKTKHRPYVTLANFPIQDPAHAGI
                     FLSHTTHLTMTPLKALQDKDFSDFLGSQKRVQKNGLFLE"
                     /name="hypothetical protein"
     gene            8019..8969
                     /gene="repM"
                     /locus_tag="MTS88_RS20115"
                     /old_locus_tag="MTS88_20115"
     CDS             8019..8969
                     /gene="repM"
                     /locus_tag="MTS88_RS20115"
                     /old_locus_tag="MTS88_20115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001205343.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHADSYINQFGVHRNTAYQALKDACKDLFARQFSYQEKKANGNIRNVMSRWVSQIAY
                     NDNEATVDLIFAPAVVPFITRLEEQFTKYELQQVSSLSSAYAIRLYELLIQWRSTGKTP
                     TIELQEFRKKLGVLDNEYLRMAHLKERVLELSIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDSPSIERDPNTLELFSKMTDAQRHMFANKLSELPEMGRYSQGTESYQQFAV
                     RIAEMLQDPAQFKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            8962..9537
                     /locus_tag="MTS88_RS20120"
                     /old_locus_tag="MTS88_20120"
     CDS             8962..9537
                     /locus_tag="MTS88_RS20120"
                     /old_locus_tag="MTS88_20120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_243327585.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTSQKIESSKQEGDESKKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATAAPHQDATACDTKMPQHATTQQDTDNKKNNELNIAENVAVPQQETTAYHTQTLQH
                     ATLQSVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            9557..9700
                     /locus_tag="MTS88_RS20125"
                     /old_locus_tag="MTS88_20125"
     CDS             9557..9700
                     /locus_tag="MTS88_RS20125"
                     /old_locus_tag="MTS88_20125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     Typing          8020..8969
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtcataaaaa aagcccgaac agggatgctc gggctataaa ctagaaacta cagcgttgaa
       61 ttataaggat aattatacag catttcgtat aaggtgtatt atgttaattt taggaaatat
      121 taaaactaat tttcagactt atcccaccaa ctttgagcta aatcagtatt tttaggaata
      181 cctataccat cacgatacat aacggaaaga cttcgcattg ctttcttatt acctaacttt
      241 gcagcctttt cacgaagttc aaaagcttta ttaatattaa cttctattcc gctatctgca
      301 cgagtatata gatcagctaa ctcaaccatt gattcagaat ggccattttc tgaggctttc
      361 tgaaaccact taaaagcttt ttggaaatgt tctaaagaat cgtttttgtc atcatcttcc
      421 attaatctag cataagtaac atatccatca gctaaccaaa acattgctgc tacatttcca
      481 ttttctgctg atttaagagc ccaaacttca gcttgatttt catctgaatc aacaccatga
      541 ccatatagat aaatctcggc taattctaac tgagcagaag tatctccatt ttctgctttc
      601 gctaataaat cattagaaaa aacaggttct gcaaatgttt ccattgaaag gcaaactcct
      661 aaagccataa aaatatttgt taatttattc aatttttaac ctttaaacta aactgttagg
      721 cttgtatatt tttatatcat acctaaaaga aaactctaaa attagagtat gtcttatctc
      781 tatttaacat aatggcgatt atacgaagta cacttgtata ttaatataaa tatcaactac
      841 ttaataaaaa tagaaaaatc ccaaaatgcc caaaaagccg actttgaaac aagtcggctt
      901 ttttatgagc agttttgata cttcttgcca ataattttga ctaaaaatta cacgaatctg
      961 gttgagtgat acaattttgt gatacattat tgtatataca aatttgaatg ttttttatgg
     1021 aacagtattt tgaatgggat gaggctaaga atcgaaagaa tcagaaaaag catgatatct
     1081 ctttcgaaac tgcaagcctt gtttttgaag atccattacg gatctcaatc caagacagac
     1141 ataccaatgg tgaagaacgt tggcaaacca ttggacgagt taaaggcgta ctaatgttat
     1201 tggtggctca caccattttt gatgaagatg actgtgaaat catccgaatc attagtgcaa
     1261 ggcaagtcac taaagcggag cgaaacaaat atgagcatgg ttagatactc acacaaagag
     1321 ctgaatgaga aatttggtga gaagcaagat gctgaaattc aacgcttgct tgctaaaggc
     1381 acggttcctg atgatcagct agatctatca gatattcctg aaattacaga ctggagcaat
     1441 gctgtacgcc aaaatcaatt ttaccgccca gtaaagcaac aaacttcaat tcggttagat
     1501 gctgatgtac ttgcttggtt caaagcacaa ggcaaaggtt atcaaacacg tatgaatgaa
     1561 attttacgag atgctatgct aaaagaatta aaaaatcatc aataagaatg ggagcttagg
     1621 ctcccatttt tgacttcatt tttagggggg attaccattc aaaaatttaa atagtagatt
     1681 ttataaattt ttaagtgtta tattataata taacattaaa tttggttgtt ttatgctaaa
     1741 taaaagtaaa ctatttttag ctttaattac tttaggggcc tcgaaaattt tattggcagc
     1801 tgaagggcct gttactacct taaatacaat agtattaact gcacaatctg atgagttagg
     1861 gtctgagctg ttaggcaaaa gtctaaatgt tagtaatcag tttattgata ctagtaaact
     1921 taaacagcgt tcgacaactc taggggatgc cttgggtacg gaattaggaa ttcattctaa
     1981 tcaatatgga ggtggagcat ctactcctat tattcgtggg caggaaggaa aacgtattaa
     2041 agttttacag aataatgctg acgtgttgga tatgtctaat atgtccccag atcacgctgt
     2101 cacagttgaa ccaagtttag ctaagtctat tgaaattata cgtggtgcaa gtaccctttt
     2161 gtatagttca aatagtgcgg caggggtagt caatgtcatt gactataaaa ttccaaccca
     2221 aatgcctcaa gacggattag agggtaatac tacattacgt tttaatactg gcagtaatga
     2281 gaagttgacc acagcaggtg taacagttgg cttaagccca catgtagctt tacgggcaga
     2341 agggttatat cgtaatgctg gtaactataa aactcctcat taccagtcta gctcttataa
     2401 ttctttagaa gatcttgaaa aggctttgtt gcataaataa atttcatccc catacatgtt
     2461 agatgaatac agaatcaaaa agccgataca aaacaacgaa ctggtctgag tacaatcagg
     2521 ctttacgtca acggggtgct ttcaccattt ggtttgatcc ccaaatgcaa tggtctgcaa
     2581 cacctactgg aaaaaaagga cgtcaaccta cctacactga tatagcaatt caatttgccc
     2641 tgacgatacg aaacctgttc caactggctt tacgccaaac acaaggtttc attcaaagct
     2701 ttttgcaaat ggctcacctc gactggaatg ttccagactt ctcaacattg agtcggcgag
     2761 cagataagct ccagccactt ctacataaat cagcaaaaaa tccacaggaa gacttgcata
     2821 ttttagttga tagcacaggg atcaaagtta caggtgatgg ggaatgggtg cgtaaaaaac
     2881 atggcgtaaa ccaacatcgt caatggctga aattacatct tgcgacagat gcaaacagtg
     2941 gagaaataca agcggttgaa gtcacgacct gccaatatgg tgatgctgaa atgctccaac
     3001 ctcttttaga tcaaattgat gaacccattg catctgtcac ggctgatggt gcttatgaca
     3061 cagtgaattg ttatgatgcg attttgaaac ggcaagcaaa tgtaattatc cctcctcgtt
     3121 ccaatgctgc tttatgggca gaaaatgaaa ttggtaaagc aaggaatcat gcagtacaag
     3181 gctgttggga caagggtcaa aagcagtgga aacgagatat tggttatcat cgacgatccc
     3241 gaatagaggc taaaatgttt gccttgaaac gacttggaca aggtgtatct agtcgatgct
     3301 ttaatcgtca ggtggttgac ttgcaaataa gagtcgatat tttgaataaa tttactcaac
     3361 ttggtacagc taaaacggtg gctgttgcat aaatatctga agattagaac aactctacct
     3421 ttcgtctatt tgtgcaacaa agcctcttga aaaccaaaac agtatttata aaaatttaaa
     3481 atatttacca gaaagctggg cagagtcgag agtaggtacg cttggattat cctggataga
     3541 cgataatact tatttaggag tttcttatac acatcgtcat gatgaatatg gtttaccagc
     3601 ccatagccat ttatatgaag gttgtggtgc ctctgcaatc ggtatcgatt cacgaattag
     3661 tggattaaaa aattatcttt tatattatcc tcaattgatg gatgagcaag atattaatta
     3721 cataaatcca cgtcctgatt gtcatcagca taatcatatt catgaaacta atttctctca
     3781 taatgctcct tatattgatt taaatactcg acgttatgat gtgcgtggag aattcactca
     3841 accatttacc ggtattgaca aaattcgtac ctcattgagt tatatagatt attttcacaa
     3901 tgaactcgag ggagataaga ttactaactt ttttaaaaat acaggaaaag taggccgcat
     3961 agagttatca catcagccat taggggaatt aaccggtatc ttggggctac aatatttgga
     4021 acaggataat agtgctttat caccagtcca ttctcaagaa ggccatacca cctatctaga
     4081 taatcagcag ctattaaatc gaaatgtcac taaaaacttt agtgtatttg gcctagaaaa
     4141 atataactgg aacgacttta ctttcgaact aggtgctcgt atagaaaagc agaaagttag
     4201 tatggattat gatatagaaa aaattaaaga ttcaatgaaa ccttggccta ataaatataa
     4261 cagtccttat gtagaaaaaa ataataaaat cagggctcaa aatttaaaat ctattttaga
     4321 agctgttcaa cctaacaaag aaacagcttt ttcttatgca ggtacagtcc actggagatt
     4381 tgctccaaat tatatcttat cattaacagg tacacatcaa gaaagattac ctaatgcaca
     4441 agaaatgtat actcatggta tgcatctcgc tactaattct tttgaaatag gtaaccgctt
     4501 tctaagaaaa gagaaatcca ataatttaga aatcagtctg gcctataagg atgatttact
     4561 tgattatcaa atcagtactt actattatga ttttgataac tatatatatt tacagacact
     4621 caatgaagtt ttaggaacta caaaagttag agatcagcat acactacgta ttaatcatta
     4681 tagccagtct gctgctaatt tttatggatt agagggcaac atagggtatc aatttaattc
     4741 tgtttatcac ggctctcttt ttggagatta tgtgaaaggc cgtttaacaa atttacctga
     4801 tgctgtaatt gcatacgata tctggaatag agaaccgact ttagctcccc aaaaggatcg
     4861 ttacactcct agattacctc ctgctcgttt gggtacacga ctaaaagctg attttgatga
     4921 atcgcttaaa ggtgaaatag aatattatcg agtctttaaa caagataata tttctaaatt
     4981 tgagcaggtt acatcaggtt ataatatgct taatatgact ttagcatata aaaataagct
     5041 atctcacaca gaatatgatt tattctttaa agcgaataat ttacttgatc agaaagtcta
     5101 tgcccatgaa acatttttgc catatattcc ccaaattgga agaaatttta gtttaggctt
     5161 aaatcttaat ttctagataa gatactgtaa tcttctaaga aaatttctta gaagattttt
     5221 cttataaaat gattagatta ataattaagg taagcgtccg ctgaatccca tacctatttt
     5281 ttaattcgat ttaggtttcc agcagtgtgg cagtaactct tcaatctggg tcactttatg
     5341 tgtcggcagc cttttcagca ctgaatagcc accgattttg tagacacctt ttagctagat
     5401 aaaaagcaat ttttttgatc tttaaagaat gaggatataa gctgtttaaa acttaattct
     5461 aattggttaa taggtgaaaa atgaaattag cagaagctct cttacttcga agtgatcaac
     5521 aaaaaaaaat tatttcacta aaacaacgta tcaatgcaaa tgtattggta caagatggtg
     5581 atcaaccatc tgaagatcca aatgaactac taaaacaagt attttcttta attcaagaat
     5641 ttcaaaaatt aagttatgca attcatgaaa ctaatgcttt aacaaaatta aatgatggcc
     5701 gttctctact tgctttattg actttgcgtg atgaatttgt agaacagcac aaaaccctaa
     5761 ctgcagcaat aagtaataca tctcgtgaat ctgatcgtta tagtacgcgg gaaataaagt
     5821 ggcataaagt tattccagtt tcatcattac aaaaacaagc tgatgatatt agtcttaaat
     5881 tacgtgattt aaacgtgtta attcagtcga ctaattggaa aattgatttg attgaagcct
     5941 aatagtcctt tagatataat caagttgaac atactgggcg agtacaagat cccttacttc
     6001 cacaacatag gattgtaaat aaagtgtagt ggcttgcaaa gcaagtggat aagcttaaaa
     6061 ttttaagtac aaatcaatcc atcacttggc ataactcaaa tctttgtgca aacaaagtta
     6121 cacgttactt ataactacca tgtactgaca gtatgttcac ctcaattact aaaaataaag
     6181 aaatgaacaa gcatcagctc aactacattt ctatttttaa ttcagttcgg catttaacat
     6241 aaaatctctt atacgaagcc caggttaatt ctgggctttt tgttcaatct catcattggc
     6301 cgagaacata cttggattcg taagcctgat attttcctga caacttccac acagcatcaa
     6361 tcctgcaaaa agttgtacat tttgtctatt ttcacaaagc gtacatggct ttgaaagctg
     6421 aataatatta tctgacacta ctttaccctc ttatagttat catctgcata tgaataacct
     6481 ttagcttgta tcattctaac ctcatcttta gaagcgccat ttttcattaa gagccacaac
     6541 atcatgaaat atagtccaga aaagtcttca tagtattttt cagaaaaatt gttatcttcc
     6601 tcaaaatcat ctattcgctt ttctaatcga ttcaccagat cctcaatacg atcggcttgc
     6661 tgtttaacag caatagattg aaaatcattg gtcaaaattt tactgaataa tttactggtt
     6721 ctgattcctt ctgaattagc caattgatcg tagaattctg ctacttgtgg atctaacctg
     6781 actgcataag ttttgacttt ttcgcccata aattacacct cttattaaaa ttagattttg
     6841 tacaaaatta aatatatata attcaactaa ttaaataaaa aataataatt agattacgta
     6901 caaacttatt tgtacagaat taatttattc taaaacaaaa acttatttaa atataatgct
     6961 ttttttatgt acaatcatga gtacatattt tttcttaaat ttatcaatat cttatctaaa
     7021 aaaagtgttg ttttgatgta caaccatgag cacagcataa aaaatagata attcaatcac
     7081 ttattctaaa aaaagaccat ttttctgtac acgtttctga gaccctaaaa aatcgctgaa
     7141 atccttatcc tgtaaggctt tgaggggtgt catcgttaga tgcgtggtgt gtgacaaaaa
     7201 aatcccggca tgtgccggat cttggattgg aaaattggct aaagtgacgt agggccggtg
     7261 cttggttttg catggaaaaa agtatttatt tccttgttta gaaaaacgta aaaaatcaat
     7321 tttttgtcca ttcatccagg ttaaaaattt caacccaaaa ctttaattat gaaaagcttc
     7381 atagaacgcg ttcaaatgcg atttaagagc ctttacctag aaaacataga tctcatagtg
     7441 aaaaacacaa aacagctcaa aaaacgcaaa tgagagcgaa ataaagaggt attttgactt
     7501 tagatagatg catagagcga gtgtctacga gcgaactatc aaaatttgcg cctagaccct
     7561 ctgaaaaaca tttttttgtc ctctttagcc taagaaagcg taagtttcgt gcagaaattt
     7621 gctcctggat cgagcgtagc gagtaaaaaa gttttatgag cgaagcgaat tccaagtttc
     7681 ttttgctttt ttttaaagtc acgccagtat taaccaaaaa attgccccaa cgaactgagc
     7741 gaaagcgaag ttcaatagag tttgagcgaa gcgaaaacca agggcaattt ttcattccct
     7801 gggcttttaa ttattttaaa gtttttaaat acttttaaat ggcttgaaag ccttatgaat
     7861 aaagggtttt agctcttata tgtccacgtt taccttgcaa tatgtccacg tttaccttgc
     7921 aatatgtcca cgtttacctt gcaatatgtc cacgtttacc ttgcattagt acacaaataa
     7981 tattaacgtg tacttataca caataaaaaa tagtggctat gagagattta gttgtaaagg
     8041 acaatgcctt aatcaacgca agctataact tagacttagt agaacaacgt ttaattttat
     8101 tggctattgt tgaagcaagg gaaagtggga aagggattaa tgcaaatgat ccattagaag
     8161 ttcatgcaga tagttatatc aatcaatttg gtgtacaccg taatacagct tatcaagcct
     8221 taaaagatgc ttgtaaggat ttattcgcgc gtcaatttag ctatcaagag aaaaaagcta
     8281 atgggaatat ccgaaatgtt atgagtcgtt gggtatctca aattgcttat aacgacaatg
     8341 aagcaactgt agacttaata tttgcacctg ctgttgttcc cttcataacc cgactggaag
     8401 aacaatttac taaatatgaa ttacagcaag ttagtagtct tagtagcgct tatgccattc
     8461 gcttatatga gcttctaatt cagtggcgaa gcactggtaa aaccccaacc atagaactac
     8521 aagaatttag aaagaagtta ggcgttcttg ataatgaata tttacggatg gctcatttaa
     8581 aagagcgtgt tttagagctt tcaattaaac aaataaatga gcatacggat ataactgtaa
     8641 aatatgaaca gcataaaaga ggacgttcta tttcaggatt ttcttttacc tttaaacaga
     8701 agaagaagga tagcccatca atagaaagag atccgaacac tttggagctt ttttcaaaga
     8761 tgaccgatgc tcaacggcat atgtttgcaa ataaactttc agaactccct gaaatgggtc
     8821 gctattcaca aggaacagaa agctaccaac agtttgctgt acgtattgct gagatgctac
     8881 aagatcccgc tcaattcaaa gagctatacc catacctaaa aaaagtggga tacatgccat
     8941 caaataaaaa ggacaccgta aatggctaag ttatcactaa gtgaagtatc taaaaaattt
     9001 catgtggata gatcaaccat ttacagagct gtacgtaatg gacgtttatc acgctccagt
     9061 gatggacaat tcgatctagc agaggtcata cgatgctttg gagaacctga gcaaacatct
     9121 caaaaaattg aatcatctaa gcaagaaggt gatgaatcta aaaaaaaact tattgcccat
     9181 ttagaaaatg aagtcaaaaa ataccaagaa cgtgaagaac ggttaatgca acaaattgac
     9241 cgtatgcaaa cactcattga gctgaaaagt gttgcacctg ccacagcagc accacaccaa
     9301 gatgctacgg catgcgacac caagatgcca cagcatgcga caacacaaca agacactgat
     9361 aacaaaaaga ataatgaatt aaatattgca gaaaatgtag cagtgccaca gcaagaaact
     9421 acggcatacc acacccaaac gctacagcat gccacgttgc aaagtgtggc agtgccacaa
     9481 cacaaaaaac gtggtttatt tggccgtgtg ctgaatgccg tctttgataa tgactgagca
     9541 ataagggtga aagatcatgc cgaaactgaa agacattgcc ctgggaatta ttgtagctcc
     9601 gctgctgatc ccgatcatgc tgattgcatc gtaccaggat aaaaaggcgc tgaagaaaga
     9661 gctggatgaa tgccaaaaag aaaaagacca ggcatcctga ttattcaatt gagctaatca
     9721 aaagtgtgga acattgatga aataatagcg tggaacgtga aaa
//
