LOCUS       NZ_CP121559             2254 bp    DNA     circular CON 17-MAY-2024
DEFINITION  Acinetobacter baumannii strain RAB9 plasmid pRAB9-3, complete
            sequence.
ACCESSION   NZ_CP121559
VERSION     NZ_CP121559.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27010269
            Assembly: GCF_029713765.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Alghorbi,M.F., Bawazeer,R., Okdah,L., Alswaji,A., Alzayer,M.A.,
            Alrashidi,E., Alshahrani,A.S., Al Johani,S.M., NGHA Surveillance
            Group,N.S.G., Balkhy,H. and Doumith,M.
  TITLE     Antimicrobial resistance and genomic characterisation of
            Acinetobacter baumannii clinical isolates from a nation
            representative surveillance in Saudi Arabia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Alghorbi,M.F., Bawazeer,R., Okdah,L., Alswaji,A., Alzayer,M.A.,
            Alrashidi,E., Alshahrani,A.S., Al Johani,S.M., NGHA Surveillance
            Group,N.S.G., Balkhy,H. and Doumith,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-APR-2023) Infectious Diseases Research, King Abdullah
            International Medical Research Center (KAIMRC), KAIMRC, P.O Box
            22490, Riyadh 11426, Saudi Arabia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: EToKi v. 1.2
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 213x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029713765.1-RS_2024_05_17
            Annotation Date                   :: 05/17/2024 05:01:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,711
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,491
            CDSs (with protein)               :: 3,491
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 60 of 125
            Pseudo Genes (incomplete)         :: 59 of 125
            Pseudo Genes (internal stop)      :: 17 of 125
            Pseudo Genes (multiple problems)  :: 11 of 125
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP121559.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="RAB9"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pRAB9-3"
                     /geo_loc_name="Saudi Arabia:Riyadh"
                     /lat_lon="24.7136 N 46.6753 E"
                     /collection_date="2018"
                     /collected_by="KAIMRC AMR WGS_Based Surveillance Program"
     gene            287..1219
                     /gene="repM"
                     /locus_tag="MTS96_RS18610"
                     /old_locus_tag="MTS96_18610"
     CDS             287..1219
                     /gene="repM"
                     /locus_tag="MTS96_RS18610"
                     /old_locus_tag="MTS96_18610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005265321.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_000711927.1"
                     /translation="MKKELVVKDNALINASYNLDLSEQRLILLAILEARQSNTPNDKDL
                     TIHAESYINHFNVHRNTAYKVLKDACKSLFDRRFSYQKLTQKGNIENVISRWVQRISYV
                     ENEALVRIKFSDDVVPLITNLEKHFTSYELEQVSSLTSVYAIRLYELLIAWRSTGKVTM
                     VELEELRLKLGIEPNEYKRMGQFKEKVLHLAIDQINKYTDIKAEYEQHKRGRSIIGFSF
                     KFKQKQQPKKLDSKRDPNTPDFFIRMTDAQRHLFGNKLAHDARVQSEYSHLIGTGSYED
                     FAKLLADMLAEEQHFKMFYPLLVEHGYKA"
                     /name="repM"
     Typing          648..1219
                     /name="rep_cluster_1280"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acgaagcgag gaacgagctt caatagagta cgagctttag cgagtacata gggcagtttt
       61 aagcactccc ctctttttaa taatttttaa ttttttaatt tttagcctct gtaattaatt
      121 gataaataat aataaaaata aggataagta cacctttatc ttgcaataag tacaccttta
      181 tcttgcaata agtacacctt tatcttgcaa taagtacacc tttatcttgc aaagtgacac
      241 ttaataaaat agagtgtact tatatactta aaagacatgt gtgcttatga aaaaagaact
      301 agtcgttaaa gataatgcac taataaatgc cagttataat ttagacctat cagaacaacg
      361 tttaatactg ttagccatac ttgaagctag gcaatcaaat acacccaatg ataaagattt
      421 gacgattcat gctgaaagct atatcaacca tttcaatgtt catagaaata cagcctataa
      481 agttcttaaa gatgcttgca aaagcctatt tgatcgtaga tttagctatc aaaaactgac
      541 tcaaaagggc aacattgaga atgtaataag tcgatgggta caacgaatat cttatgtaga
      601 gaatgaagct cttgttcgta ttaagttttc tgatgatgtt gtaccgttga ttacaaattt
      661 agaaaaacac ttcactagtt atgaactaga gcaagtcagc agtttaacta gtgtttacgc
      721 tatacgcttg tatgagttgc ttatcgcatg gcgtagtacg ggcaaagtga ctatggtaga
      781 gctagaagag ctaagattaa aactaggcat agaacccaat gaatacaaga gaatggggca
      841 atttaaagaa aaagttttac accttgccat tgatcaaatt aataagtaca cagatataaa
      901 agcagagtat gaacaacaca aacgtggtcg ttcaattatt ggtttttcat ttaagtttaa
      961 gcagaaacaa caaccgaaaa aattagattc taagcgtgac ccaaacacac ctgacttttt
     1021 tatcagaatg accgatgcac aacgccactt atttgggaac aaactagccc atgatgctcg
     1081 tgtacaaagc gaatacagcc atttaattgg tactggaagc tatgaagact ttgcaaaatt
     1141 actggcggat atgcttgcag aagaacagca ttttaagatg ttttaccctt tgcttgtaga
     1201 gcatggttac aaagcataaa tcttgtcgta ttctatcaat ggtagataag tcatttcgac
     1261 cttccttatc tatgtacaag ataaggtggc gccaccaata cgccaatatt ggtggatagc
     1321 caccaacccc tctaaattgc gttttaagcg atccaaatcc aacaagctac attcacatac
     1381 ccactgcgag caaagctcga aatatcgcat cctatgagca aatagagcat gtaatcaggg
     1441 agggtgtgct tgtttttgca tttcatcagt tcactcgtag acactcgctc taaaaatcta
     1501 tctgtgcttt tcattttggc tttagccaaa gaaagccccg aaggggtgtg aagcgaaagc
     1561 ggaacatcat cgaagcccca gcggagatga ctggggggag ccgacaacgt aaaagggagt
     1621 ggacacgaac tttagacatc cccagatgtc gttagttgtt ggggggcaag cccaccccgt
     1681 caaactatcc cgaaggggag ttgacccacc tcgttttagt gtaagcgttc agcgaatccg
     1741 aaggacatac gagcctaagc gaaatatgca cctaaaaggg ggtgggtcaa ctccgtagga
     1801 ggatgacggg gcgcgagcac gaagtgcgct gcaacttgga gcgatagcgg aaagtgaaag
     1861 gcaacaaaaa acgattatta agggcgtcca gcccttaaag tacaaggaat cctgacgtcc
     1921 aggaaggacg agcgaagcga gcttgtacgg taattgtttt taggcgcagc gaagcgaagt
     1981 gtctgtgcct tttactgtgc ttttatcggt gcactaaaaa tccaataaaa gcaccataga
     2041 aagcacagat agaattaacc gagcgagtgt ctacgagcga ttgagcatca tcaaaatttc
     2101 gccctcgcct tttctctgat tacaagctcc ctttcgattt taaacggtag gtacagagca
     2161 tcccgaatgg gtgcgaactt tggagctttt gcttttcaaa aagtatgagc gaagcgaata
     2221 cattacaaat gcttttgctt ttagatttca caaa
//
