LOCUS       NZ_CP121220             7402 bp    DNA     circular CON 29-JUN-2023
DEFINITION  Shigella flexneri 2a strain Sflex 21-42 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP121220
VERSION     NZ_CP121220.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33861938
            Assembly: GCF_029625495.1
KEYWORDS    RefSeq.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7402)
  AUTHORS   Ritchie,G., Leung,V., Himsworth,C., Byers,K., Lee,L., Chorlton,S.,
            Stefanovic,A., Romney,M., Matic,N. and Lowe,C.
  TITLE     No isolate, no problem: Using a novel insertion sequence PCR to link
            rats to human shigellosis cases in an underserved urban community
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7402)
  AUTHORS   Ritchie,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Pathology and Laboratory Science, University
            of British Columbia, 1081 Burrard Street, Vancouver, BC V6Z1Y6,
            Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 21-SEP-2021
            Assembly Method                   :: metaFlye v. SEP-2021
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 70.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/29/2023 08:22:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,088
            CDSs (total)                      :: 4,956
            Genes (coding)                    :: 4,188
            CDSs (with protein)               :: 4,188
            Genes (RNA)                       :: 132
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 103
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 768
            CDSs (without protein)            :: 768
            Pseudo Genes (ambiguous residues) :: 0 of 768
            Pseudo Genes (frameshifted)       :: 279 of 768
            Pseudo Genes (incomplete)         :: 450 of 768
            Pseudo Genes (internal stop)      :: 173 of 768
            Pseudo Genes (multiple problems)  :: 129 of 768
            Pseudo Genes (short protein)      :: 5 of 768
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP121220.1.
            Bacteria and source DNA available from Willson Jang, St. Paul's
            Hospital Microbiology Laboratory, 1081 Burrard Street, Vancouver.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7402
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /strain="Sflex 21-42"
                     /serotype="2a"
                     /isolation_source="fecal sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /plasmid="unnamed3"
                     /geo_loc_name="Canada: Vancouver"
                     /lat_lon="49.28 N 123.12 W"
                     /collection_date="2021-03-23"
                     /collected_by="St. Paul's Hospital"
     gene            complement(join(<7402..<7402,1..751))
                     /locus_tag="P5F89_RS00030"
                     /old_locus_tag="P5F89_00030"
                     /pseudo=""
     CDS             complement(join(<7402..<7402,1..751))
                     /locus_tag="P5F89_RS00030"
                     /old_locus_tag="P5F89_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(741..1064)
                     /locus_tag="P5F89_RS00035"
                     /old_locus_tag="P5F89_00035"
     CDS             complement(741..1064)
                     /locus_tag="P5F89_RS00035"
                     /old_locus_tag="P5F89_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000956004.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1129..1320
                     /locus_tag="P5F89_RS00040"
                     /old_locus_tag="P5F89_00040"
     CDS             1129..1320
                     /locus_tag="P5F89_RS00040"
                     /old_locus_tag="P5F89_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165994.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2397..2789
                     /locus_tag="P5F89_RS00045"
                     /old_locus_tag="P5F89_00045"
     CDS             2397..2789
                     /locus_tag="P5F89_RS00045"
                     /old_locus_tag="P5F89_00045"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_128567429.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQI
                     QEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGNNA
                     ETQSTHYQPYPYDKVSIWGPFPHNLT"
                     /name="hypothetical protein"
     gene            complement(3335..3568)
                     /locus_tag="P5F89_RS00050"
                     /old_locus_tag="P5F89_00050"
     CDS             complement(3335..3568)
                     /locus_tag="P5F89_RS00050"
                     /old_locus_tag="P5F89_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137443.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3575..4105)
                     /locus_tag="P5F89_RS00055"
                     /old_locus_tag="P5F89_00055"
     CDS             complement(3575..4105)
                     /locus_tag="P5F89_RS00055"
                     /old_locus_tag="P5F89_00055"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067502.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSG
                     GTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETNWTM
                     ENGKQNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4143..4574)
                     /locus_tag="P5F89_RS00060"
                     /old_locus_tag="P5F89_00060"
                     /pseudo=""
     CDS             complement(<4143..4574)
                     /locus_tag="P5F89_RS00060"
                     /old_locus_tag="P5F89_00060"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4783..5106)
                     /locus_tag="P5F89_RS00065"
                     /old_locus_tag="P5F89_00065"
     CDS             complement(4783..5106)
                     /locus_tag="P5F89_RS00065"
                     /old_locus_tag="P5F89_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000956004.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5171..5362
                     /locus_tag="P5F89_RS00070"
                     /old_locus_tag="P5F89_00070"
     CDS             5171..5362
                     /locus_tag="P5F89_RS00070"
                     /old_locus_tag="P5F89_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165994.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6439..6831
                     /locus_tag="P5F89_RS00075"
                     /old_locus_tag="P5F89_00075"
     CDS             6439..6831
                     /locus_tag="P5F89_RS00075"
                     /old_locus_tag="P5F89_00075"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_128567429.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQI
                     QEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGNNA
                     ETQSTHYQPYPYDKVSIWGPFPHNLT"
                     /name="hypothetical protein"
     Typing          complement(1340..1426)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.43e-38"
     Typing          complement(5382..5468)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.43e-38"
     Typing          1437..1994
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.032"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5479..6036
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.032"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4..734)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.131"
                     /coverage="100.0"
                     /evalue="6.26e-154"
     Typing          complement(745..1097)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.01e-70"
     Typing          complement(3232..4793)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.313"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tagattcggc tctctctttc agctttttct cgaactccgc gcccgtctgt aaaagattgc
       61 tcatagaatg ctcctttcag tctgatattc ttcccgccgt tcgggtctgc aatgctgata
      121 ctgcttcgcg tcaccctgac cacttcaagc cccgcctctg tgagcgcctg aatcacatcc
      181 tgacggcctt ttatctcccc gacatgataa agagcgtcta tccaccgtgt aatgctctca
      241 gcaagcgcct gtttcgtttc cggcaggtta tcagggagag tcagcgtccg gcggttctcc
      301 ggggcgttcg ggtcatgcag cccgtaatgg tgattcacca gcgtctgcca ggcatcaatt
      361 ctcggcctgt ctgcgcggtc gtagtacggc tggaggcgtt ttccggtctg tagctccatg
      421 ttcggaatga caaaattcag ctcaagccgc cccttgtcct ggtgctccac ccacagaatg
      481 ctgtactgat ttttttcgag accgggcatc agtacacgct caaagctcgc catcactttt
      541 tcacgccctc ccggcggcag ctccttctcc gcgaacgaga gaacaccgga cgtgtatttc
      601 ttcgcaaatg gcgtggcatc gatgagttcc cggacttctt ccggattacc ctgaagcacc
      661 gttgcccctt cccggttacg ctcccttccc agcaggtaat caaccggacc actgccaccg
      721 ccttttccct ggcatgaaac ttaactatca tcccgcgtcc cctgttccct gacggccaga
      781 cgcagccggc gcagctcatc cccgatggcc atcagtgcgg ccaccacctg aacccggtca
      841 ccggaagacc actgcccgct gttcaccttc ctcgctgcct gattcaggtt attcccgatg
      901 gcggccagct gacgcagtaa cggcggtgcc agtgtcggca gcctcccgga acgggcaacc
      961 ggctcaccca ggcagacccg ccgcatccat accgccagct gtttaccctc acagcgttcc
     1021 agtaaccggg catgttcatc atcagtaacc cgtatcgtga gcatcctctc gcgtttcatc
     1081 ggtatcatta cccccatgaa cagaaatccc ccttacacgg aggcatcagt gaccaaacag
     1141 gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc agacattaac gctgctggag
     1201 aagctcaacg aactggacgc ggatgaacag gcagacatct gtgaatcgct ttacgaccac
     1261 gccgatgagc tttaccgcag ttgcctcgct cgcttcgggg atgacggtga aaacctctga
     1321 cgggcttgtc agcgggtgtt agcgggtgtc ggggcgcagc catgacccag tcacgtagcg
     1381 atagcggagt gtatactggc ttaaccatgc ggcatcagtg cggattgtat gaaaagtgcg
     1441 ccataccggg tgtgaaatgc cgcacagatg cgtaaggaga aaatgcacgt ccaggcgctt
     1501 ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cgactgcggc gagcggtatc
     1561 tactcactca aaaacgaaat gctgttatcc acagaatcag gggataacgc cggaaagaac
     1621 atgtgagcaa aagaccagga acaggaagaa ggccgcgccg atggcgtttt tccataggct
     1681 ccgcccccct gacgagcatc acaaaaaccg acgctcaagt cagaggtggc gaaacccgac
     1741 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     1801 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     1861 tcatagctca cgctgttggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     1921 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     1981 gtccaacccg gtaagacacg acttaacgcc actggcagca gccattggta actggacaag
     2041 gtggattgag atattaagag ttcttgaagt ggtggcctaa ctgcggctac actagaagga
     2101 cagcatttgg tatctgtgct ccaccaagcc agttaccttc ggaagaaaga gttggtagct
     2161 ctagattccg gcaaacaaac caccgttggt agcggtggtt tttttgtttg caggcagcag
     2221 attacgcgcc gcaaaaaagg atctcaagaa gatcttgaat cactctatga aaattttgtt
     2281 ttatagccgt atttgttgat gctataagcc agagaccttc tatgagaata gacatatcac
     2341 atcaaacacg gcataccccg ccaaacatgc ttcctcggga acaaaattgt gtagcaatgg
     2401 cattatctgc gtgctttaga caacaactaa accctgtggt aaacagctta ttaaaagaga
     2461 gaataataca ctcccctaaa gaattggaac atgacaacgc agtaataaga gtcctgcaaa
     2521 aactacaaat tcaagaagtt tgtaacagca ccctttggga gacagccaaa caacaactac
     2581 ttcaaaaatc ggacggaaga tattttgcca tcaattctaa acacttagcc tttccaggcc
     2641 caggagaatc tcacgccttc tgttgcatca aatacaaaaa tgcaattggc attaacggaa
     2701 acaatgccga aacccaatcg acccactacc aaccctatcc ctatgataaa gtcagtatat
     2761 ggggaccatt ccctcacaat ttaacgtaat atcttaattg tacgcaccgc tgaaacgcgt
     2821 tcggcgcgat cacggcagca gacaggtaaa aatggcaaca aaccacccga aaaactgccg
     2881 cgatcgcacc cggtaaattt taaccacatg catagctatg cagccatgtg aatcacgcag
     2941 gaattgccag ccagagacag ctgaaacgga ttttattcat gctttcggaa gcgaagaaga
     3001 cggggacctg agcgggaaaa cagaagttca ggggaatgag cgcgatctgg caatagaggc
     3061 aaaacacagc gacaaaagac acaccagaat cgcgcccgta tgcgttttaa ctcgttcctg
     3121 tgttttcagc aaggtctgac tgaagcgcgt tcccggtgcc cccgaaccaa ccattatccc
     3181 aaaaaatgca ccggaaacgc gctgacaaca ttttagcctg tctctgatta ccatcccagc
     3241 gaagggccat ccagtgtccg ttcctgtatt tccggctggc gttcccgttc caggaataac
     3301 gcatgttcgc gctcctgtat caaccgttcc tctcttatct ccagttctcg ctgtataact
     3361 ggctcaagcg ttctgtctgc tcgctcaagt gcagcacctg ctgactcaac tgcatgaccc
     3421 gctcgttcag catcgcgttg tcccgttgcg taagcgaaaa cattttctgc aattccacga
     3481 aggcgctctc ccattcgttc agccgctgca tatagtcctg ttgcagctgc tctaatgcgt
     3541 tcagcaaatg gctttccagc tccgtcatgc tctgtcactc ctgtaccagc cttacggcat
     3601 tctgtttccc gttttccatc gtccagttcg tttcggcttt catcccctcg ggcaacacca
     3661 gaaaacgtcc gttgctcccc tcgtgataac gcacgcccca tgtcttcgcg ttcagcttct
     3721 ccagactctc gttctgcttc ctgatttctt ccaggttcgc ctgtatcatc ccgccaagat
     3781 accagagcgt cccgccactc acggtaaaca ggaaaatgac catccccagt aacatcatgc
     3841 ccgtattccc tgccagcttt aacacgtcct tcctgtgctg catcatcgcc tctttcaccc
     3901 cttcccgatg ttttttcagt gattcctctg tcgaagctgt gaacagggct atagcgtctt
     3961 tgattttcgt ctcgtttgat gtcacagcct tgcttacaga ttcttcgagc ttcctgaact
     4021 cgtcgctcag cattttctct gtagattcgg ctctctcttt cagctttttc tcgaactccg
     4081 cgcccgtctg taaaagattg ctcatagaat gctcctttca gtctgatatt cttcccgccg
     4141 ttcgggtctg caatgctgat actgcttcgc gtcaccctga ccacttcaag ccccgcctct
     4201 gtgagcgcct gaatcacatc ctgacggcct tttatctccc cgacatgata aagagcgtct
     4261 atccaccgtg taatgctctc agcaagcgcc tgtttcgttt ccggcaggtt atcagggaga
     4321 gtcagcgtcc ggcggttctc cggggcgttc gggtcatgca gcccgtaatg gtgattcacc
     4381 agcgtctgcc aggcatcaat tctcggcctg tctgcgcggt cgtagtacgg ctggaggcgt
     4441 tttccggtct gtagctccat gttcggaatg acaaaattca gctcaagccg ccccttgtcc
     4501 tggtgctcca cccacagaat gctgtactga tttttttcga gaccgggcat cagtacacgc
     4561 tcaaagctcg ccatcacttt ttcacgccct cccggcggca gctccttctc cgcgaacgag
     4621 agaacaccgg acgtgtattt cttcgcaaat ggcgtggcat cgatgagttc ccggacttct
     4681 tccggattac cctgaagcac cgttgcccct tcccggttac gctcccttcc cagcaggtaa
     4741 tcaaccggac cactgccacc gccttttccc ctggcatgaa acttaactat catcccgcgt
     4801 cccctgttcc ctgacggcca gacgcagccg gcgcagctca tccccgatgg ccatcagtgc
     4861 ggccaccacc tgaacccggt caccggaaga ccactgcccg ctgttcacct tcctcgctgc
     4921 ctgattcagg ttattcccga tggcggccag ctgacgcagt aacggcggtg ccagtgtcgg
     4981 cagcctcccg gaacgggcaa ccggctcacc caggcagacc cgccgcatcc ataccgccag
     5041 ctgtttaccc tcacagcgtt ccagtaaccg ggcatgttca tcatcagtaa cccgtatcgt
     5101 gagcatcctc tcgcgtttca tcggtatcat tacccccatg aacagaaatc ccccttacac
     5161 ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac atggcccgct ttatcagaag
     5221 ccagacatta acgctgctgg agaagctcaa cgaactggac gcggatgaac aggcagacat
     5281 ctgtgaatcg ctttacgacc acgccgatga gctttaccgc agttgcctcg ctcgcttcgg
     5341 ggatgacggt gaaaacctct gacgggcttg tcagcgggtg ttagcgggtg tcggggcgca
     5401 gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaaccat gcggcatcag
     5461 tgcggattgt atgaaaagtg cgccataccg ggtgtgaaat gccgcacaga tgcgtaagga
     5521 gaaaatgcac gtccaggcgc ttttccgctt cctcgctcac tgactcgcta cgctcggtcg
     5581 ttcgactgcg gcgagcggta tctactcact caaaaacgaa atgctgttat ccacagaatc
     5641 aggggataac gccggaaaga acatgtgagc aaaagaccag gaacaggaag aaggccgcgc
     5701 cgatggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaac cgacgctcaa
     5761 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     5821 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     5881 cttcgggaag cgtggcgctt tctcatagct cacgctgttg gtatctcagt tcggtgtagg
     5941 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     6001 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttaacg ccactggcag
     6061 cagccattgg taactggaca aggtggattg agatattaag agttcttgaa gtggtggcct
     6121 aactgcggct acactagaag gacagcattt ggtatctgtg ctccaccaag ccagttacct
     6181 tcggaagaaa gagttggtag ctctagattc cggcaaacaa accaccgttg gtagcggtgg
     6241 tttttttgtt tgcaggcagc agattacgcg ccgcaaaaaa ggatctcaag aagatcttga
     6301 atcactctat gaaaattttg ttttatagcc gtatttgttg atgctataag ccagagacct
     6361 tctatgagaa tagacatatc acatcaaaca cggcataccc cgccaaacat gcttcctcgg
     6421 gaacaaaatt gtgtagcaat ggcattatct gcgtgcttta gacaacaact aaaccctgtg
     6481 gtaaacagct tattaaaaga gagaataata cactccccta aagaattgga acatgacaac
     6541 gcagtaataa gagtcctgca aaaactacaa attcaagaag tttgtaacag caccctttgg
     6601 gagacagcca aacaacaact acttcaaaaa tcggacggaa gatattttgc catcaattct
     6661 aaacacttag cctttccagg cccaggagaa tctcacgcct tctgttgcat caaatacaaa
     6721 aatgcaattg gcattaacgg aaacaatgcc gaaacccaat cgacccacta ccaaccctat
     6781 ccctatgata aagtcagtat atggggacca ttccctcaca atttaacgta atatcttaat
     6841 tgtacgcacc gctgaaacgc gttcggcgcg atcacggcag cagacaggta aaaatggcaa
     6901 caaaccaccc gaaaaactgc cgcgatcgca cccggtaaat tttaaccaca tgcatagcta
     6961 tgcagccatg tgaatcacgc aggaattgcc agccagagac agctgaaacg gattttattc
     7021 atgctttcgg aagcgaagaa gacggggacc tgagcgggaa aacagaagtt caggggaatg
     7081 agcgcgatct ggcaatagag gcaaaacaca gcgacaaaag acacaccaga atcgcgcccg
     7141 tatgcgtttt aactcgttcc tgtgttttca gcaaggtctg actgaagcgc gttcccggtg
     7201 cccccccccc gaaccaacca ttatcccaaa aaatgcaccg gaaacgcgct gacaacattt
     7261 tagcctgtct ctgattacca tcccagcgaa gggccatcca gtgtccgttc ctgtatttcc
     7321 ggctggcgtt cccgttccag gaataacgca tgttcgcgct cctgtatcaa ccgttcctct
     7381 cttatctcca gttctcgctg ta
//
