LOCUS       NZ_CP121000             3091 bp    DNA     linear   CON 17-AUG-2023
DEFINITION  Streptomyces sp. Mut1 plasmid unnamed3.
ACCESSION   NZ_CP121000
VERSION     NZ_CP121000.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33694643
            Assembly: GCF_030719295.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. Mut1
  ORGANISM  Streptomyces sp. Mut1
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Widen,T. and Larsbrink,J.
  TITLE     Isolation and description of six Streptomyces strains from soil
            environments, able to metabolize different microbial glucans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Widen,T. and Larsbrink,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAR-2023) DNASense, DNASense ApS, Niels Jernes Vej 10,
            Aalborg 9220, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9.1-b1780
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 469x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/15/2023 08:25:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,849
            CDSs (total)                      :: 6,758
            Genes (coding)                    :: 6,668
            CDSs (with protein)               :: 6,668
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 71 of 90
            Pseudo Genes (internal stop)      :: 7 of 90
            Pseudo Genes (multiple problems)  :: 19 of 90
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP121000.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Streptomyces sp. Mut1"
                     /mol_type="genomic DNA"
                     /strain="Mut1"
                     /isolation_source="soil [ENVO:00001998]|soil organic matter
                     [ENVO:04000008]|plant litter [ENVO:01000628]|plant matter
                     [ENVO:01001121]"
                     /db_xref="taxon:3033811"
                     /plasmid="unnamed3"
                     /geo_loc_name="Sweden: Gothenburg"
                     /lat_lon="57.689951 N 11.950568 E"
                     /collection_date="2022-08-02"
     gene            <1..295
                     /locus_tag="P8A18_RS34235"
                     /old_locus_tag="P8A18_34235"
                     /pseudo=""
     CDS             <1..295
                     /locus_tag="P8A18_RS34235"
                     /old_locus_tag="P8A18_34235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951564.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=2
                     /transl_table=11
                     /product="mob protein"
                     /name="mob protein"
     gene            complement(325..783)
                     /locus_tag="P8A18_RS34240"
                     /old_locus_tag="P8A18_34240"
     CDS             complement(325..783)
                     /locus_tag="P8A18_RS34240"
                     /old_locus_tag="P8A18_34240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125938.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_001125937.1"
                     /translation="MPKQKTENEIIYNGKSRWRNLDTGEIIETDEVIKKTPRNGFMITY
                     LTAIVQLIDSLGNKKMQVVKYILENMDKSTNTLIITTRELTEKSKVSRQTVIDTLKTLE
                     QAQIITRRTGAIMIHPSLVHRGKDTKEKYLLARFEDFNNNKAPINAVE"
                     /name="replication/maintenance protein RepL"
     gene            2073..>3091
                     /gene="mobV"
                     /locus_tag="P8A18_RS34245"
                     /old_locus_tag="P8A18_34245"
     CDS             2073..>3091
                     /gene="mobV"
                     /locus_tag="P8A18_RS34245"
                     /old_locus_tag="P8A18_34245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951564.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_306061741.1"
                     /translation="MLDQIIGFSQSSCYADYQKGGLLVAHVEKYTKGSVQGLSIHWDRK
                     TENHSNQEIDNERSNLNYDLCEKEGDTLSRMNDRLREVHCLNRKDVKVCSDWVVTLPEN
                     LKGTSEKEQREFFEKTYEFLANRYGGEKNVLSANVHKDETTPHMHFAFMPVVWDEKKQR
                     EKVSAKEVLTRKDLKTFHQDLDTFLKKEIPHIYKDGILNDKTIGVDTVKDLKKHSLEIE
                     KQKKAMDAEIKIKEKDLERKIQSLDKEFESKKKKLLDLSEQLPQEIKINAKGKEKKTEV
                     VKKGLFKTETITKNTGNWIIGTNELKRVQKMVNAAYMVKRDYERLQSTDLVEENKKLHL
                     "
                     /name="mobV"
     Typing          complement(326..783)
                     /name="rep_cluster_1950"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2002..3089
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.725"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gcaagtagag ggcttgtcta acaatttaaa agcatctcat cagattaatg cggagttgag
       61 agagagaaat aaagaattac atacaaaaat aggctcgtta caagctcata taaacgattt
      121 gaagataaac gtaaaggttt tatatcaaca aacgaaaaaa gtctttaaag agcaatttaa
      181 aacgtttaga gggcttgtta agaatgaact agttggaagg gaagtagagg attactttga
      241 gcgtgaacat aaaaacgaaa tgactaaaca acgaggatat gatatggaaa ggtaaaaccg
      301 ccttagaatc aattcttggg cggtttattc aactgcatta attggagcct tattattatt
      361 aaaatcttcg aatctagcaa gtaagtattt ctcttttgta tctttaccac gatgaacaag
      421 gcttggatgt atcattattg ctccagttct tctagtaatt atttgggctt gctccagtgt
      481 ttttaatgtg tctattacag tttgtctaga taccttgctt ttttcggtca attctcttgt
      541 agttataatt aacgtatttg ttgatttgtc catgttttct aatatgtatt ttacaacttg
      601 cattttttta ttacctaaac tatctattaa ttgaacgatt gcagttaaat atgtgatcat
      661 aaaaccattt ctaggagttt tctttatgac ttcatcagtt tctattattt cacctgtatc
      721 taggtttctc catcgacttt taccgttata tattatttcg ttttcagttt tttgttttgg
      781 catattatca cctttctcct atatttttat atttccattt tatcataatt agaccaaaag
      841 gtcaaaaaaa aatagaccaa aaggtataaa aaattagacc aaaaagtata aaaaattaga
      901 ccaaagaaaa tgcccttcaa tagctatata tcagcttaaa atctactttt taccccctgc
      961 ttactctttt ctatatattt aaggcgataa atatcgcctt aaatatattc ttttttcatt
     1021 ttttttagag ggctttccac aatgcaatag tcgagtagta ttttttgaga aaaccactca
     1081 aaaaatctcc actcttttgc tattgcatag tgaaattcgg caggtgtgag catgtcgcca
     1141 agccgaacat accaaaaacg aggatttaag ttgcacgact tggactagca attgtacctg
     1201 ccgaaccgcc ttgatctatc gttcaagaat ttcactaatg ccatataaaa ttacgttaag
     1261 ttcctggggg ctagccccca aacccccagc cagcactcac acccaaaaac aagggggata
     1321 ccaataaaaa agtcaaaatc cactatatag gattttgact tttttattgg tattgaacta
     1381 caagggtccc tacaggctgt ttttgaacac ccgtagttct ttttaaaagg gattaattta
     1441 tcagatttat taaatgaata attcataaaa tcccctttca atcactctgt tgtatggcaa
     1501 tttatcaagt aggcagttgt acataagtag tgtatgcagg ggacaaaaat gtgtatataa
     1561 ctggataagc cttttttatt tttggcttgg tatgaaaaaa gaaccctaat gttggatagg
     1621 gttcttttta ggggtaacag ttgaaggttg aaggtatatt tcatatttat tatgtacttt
     1681 actcttatat tcattcgaat aggaaacaca tatgtttgga gtaatttgca aagcgattgt
     1741 tggaaaagga aaaattagcg gtaagtgtca atggtaagcg gactggaagg ggtcagcggt
     1801 ttgtgctgat ccatgaaagg agcattaagg aattgacacg tgcaattgat tcttcaccct
     1861 cttggcctgt caaaacgtca aacgttttgg cctgccaacc ggcgagggag tccctcaagg
     1921 attgaggggt tggggagttc gattgatggg gtcaagggga agagttcccc ttgtaggttc
     1981 ggacaaagtc cgaggttttt ttgccatgca aggcatggtt tggcgaagcc aacgagcctc
     2041 gcagagcacg tccaaatgaa atttggtata acgtgctaga ccaaattatt ggattctctc
     2101 aatcttcgtg ctatgctgat taccaaaagg ggggcttact tgtggctcat gtagaaaaat
     2161 atacaaaagg cagcgtacaa ggattatcga ttcattggga cagaaaaaca gaaaatcatt
     2221 cgaatcaaga gattgataat gagcggtcaa atttaaatta tgatttatgc gaaaaagaag
     2281 gagatacact ttcccgcatg aatgatcgat tacgtgaagt acattgtttg aatcggaaag
     2341 atgtaaaagt ttgtagtgat tgggttgtta cgttaccaga aaacttgaag gggacttcag
     2401 aaaaagaaca acgtgaattt tttgaaaaaa cctatgagtt tttagcgaat cgttatggcg
     2461 gtgaaaaaaa tgtattgtcc gctaacgtac ataaggacga aacaacacct catatgcatt
     2521 ttgcttttat gcctgttgtt tgggacgaga aaaaacaacg ggaaaaagtt tcagccaaag
     2581 aggttttaac aagaaaagat ttaaagacat tccaccaaga tttagataca tttttgaaaa
     2641 aggaaatacc acacatctac aaagatggaa ttttaaatga taaaacgatt ggtgttgata
     2701 ccgttaagga tttaaaaaag cattctctag aaatagagaa acaaaaaaag gcgatggatg
     2761 cggaaataaa gataaaagaa aaagacttgg agagaaaaat ccaatcttta gataaagaat
     2821 ttgaaagtaa aaagaaaaag cttttagatc tcagtgaaca attaccacaa gaaatcaaaa
     2881 tcaatgcaaa agggaaagag aaaaaaacag aggttgtgaa aaaaggatta tttaaaacag
     2941 aaacgataac caaaaacact gggaattgga ttattggaac gaacgaattg aaaagagtac
     3001 aaaaaatggt gaacgctgcc tatatggtca aaagggatta tgaacgtttg caaagtacag
     3061 atttggtgga agaaaataaa aaattacacc t
//
