LOCUS       NZ_CP120999            11634 bp    DNA     linear   CON 17-AUG-2023
DEFINITION  Streptomyces sp. Mut1 plasmid unnamed2.
ACCESSION   NZ_CP120999
VERSION     NZ_CP120999.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33694643
            Assembly: GCF_030719295.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. Mut1
  ORGANISM  Streptomyces sp. Mut1
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 11634)
  AUTHORS   Widen,T. and Larsbrink,J.
  TITLE     Isolation and description of six Streptomyces strains from soil
            environments, able to metabolize different microbial glucans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11634)
  AUTHORS   Widen,T. and Larsbrink,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAR-2023) DNASense, DNASense ApS, Niels Jernes Vej 10,
            Aalborg 9220, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9.1-b1780
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 469x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/15/2023 08:25:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,849
            CDSs (total)                      :: 6,758
            Genes (coding)                    :: 6,668
            CDSs (with protein)               :: 6,668
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 71 of 90
            Pseudo Genes (internal stop)      :: 7 of 90
            Pseudo Genes (multiple problems)  :: 19 of 90
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP120999.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11634
                     /organism="Streptomyces sp. Mut1"
                     /mol_type="genomic DNA"
                     /strain="Mut1"
                     /isolation_source="soil [ENVO:00001998]|soil organic matter
                     [ENVO:04000008]|plant litter [ENVO:01000628]|plant matter
                     [ENVO:01001121]"
                     /db_xref="taxon:3033811"
                     /plasmid="unnamed2"
                     /geo_loc_name="Sweden: Gothenburg"
                     /lat_lon="57.689951 N 11.950568 E"
                     /collection_date="2022-08-02"
     gene            <1..561
                     /locus_tag="P8A18_RS34195"
                     /old_locus_tag="P8A18_34195"
     CDS             <1..561
                     /locus_tag="P8A18_RS34195"
                     /old_locus_tag="P8A18_34195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018560448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_306061724.1"
                     /translation="GNAQAYPIYRSFDVASVTCVASELVRCGDRQEGKTGYFDAELRDV
                     AGVLAARDLFPGAWFSHGGSDFRGADLPSDVAALAGLLPLQLDAEDLLEGETLSRAVTV
                     LGSAVWGQLAWMALTWPAVPELGLGPEHARTGVQACFNIDANHEPVAGHTVYVHVHPGD
                     EDRARHLARTVGQDLIGPPEYGW"
                     /name="hypothetical protein"
     gene            614..3286
                     /locus_tag="P8A18_RS34200"
                     /old_locus_tag="P8A18_34200"
     CDS             614..3286
                     /locus_tag="P8A18_RS34200"
                     /old_locus_tag="P8A18_34200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003978821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="WP_306061726.1"
                     /translation="MLKSSIRLREHQVDQRNAFRKWVGFPARSSVPPQGARGTIVSATG
                     SGKTITAAACALESFPDGRILVTVPTLDLLAQTAQAWRAVGHRAPMVAVCSLENDAVLN
                     SLGVRTTTNPIQLALWAGTGPVVVLATYASLVDREDITATTGQGKVRGPLEAALAGGER
                     LYGQQMDPFDLTIVDEAHGTAGELARPWAAIHDNARIPADFRLYLTATPRILAAPRPQR
                     GAGGQEAEIASMADDPDGTYGAWLAELGLSEAIEREILAGFEIDVLEIRDPSPVLGESE
                     EARRGRRLALLQTALLEHAAAYNLRTVMTFHQKVEEARTFADKLPETAAALYLNDASDA
                     DLAAADKLPKSSIDAEFYELEAGRHVPPDRVWSAWLCGDHLVSERREALRQFANGIDAA
                     GRRVHRAFLASVRVLGEGVDITGERGVDSICFADTRGSQVEIVQNIGRALRLNKDGSTK
                     VARIIVPVFLEPNEDPTDMVASASFRPLVAILQGLRSHDDRLVEQLASRALTSGKRKIH
                     VRRDEDGQIVGAGGAGDGEDQEEEDDDTQAAAEAALLHFSSPRDPATIAAFLRTRVYRP
                     ESLVWLEGYQALIRWRKENEITGLYAVPYDIEIEVGATKAFPLGRWVHQQRKALRAGEL
                     EERRKTLLDAPEAGMVWEPGEEAWENKLAALRSYRRAMGHLAPRQDAVWGEGEGEAMVP
                     IGQHLANLRRKGGLGKDAERARRRAAQLTEIDPDWDCPWPLDWQRHYRVLADLVDADGH
                     LPDIAPGVAFDGDDLGKWLARQRKAVTWAQLSTAQQERLSQLGLKPIQPPSPATAATRA
                     TKPGKAQQAFQRGLTALAQYVAREGESTVKRTHREKIVIDGQEHDLALGIWYSNQKQRR
                     DKLTAEQLDALRELGMEWA"
                     /name="DEAD/DEAH box helicase"
     gene            3465..6389
                     /locus_tag="P8A18_RS34205"
                     /old_locus_tag="P8A18_34205"
     CDS             3465..6389
                     /locus_tag="P8A18_RS34205"
                     /old_locus_tag="P8A18_34205"
                     /inference="COORDINATES: protein motif:HMM:NF018259.2"
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase associated domain-containing protein"
                     /protein_id="WP_306061728.1"
                     /translation="MAPLQGEMTQSFLSRVAARYGVRLRDLLAAIAEVGGLSNITGQTR
                     LDSEVYLNRQARDRVSQLCRVPERHLRRALPAWEQEEPRKRFASGPAAQFHHTAEKVVP
                     WGPACPECAAQSAGRAECARLYLEPQQRVCALHRRWLMQVPGTAGRVVRLPTGGEQWVQ
                     AHRRHARLLRRSSVAVEAFEVAAAVTASWWWQAWSREHVWSSRLRSLGSGRMDPEVWRV
                     LARELVTYPETVALATLLADDAFRQCLIADARGHLPFRLADLPVLLSAVARCVRRSWYR
                     EQLADETSGPLFAWAYQCVRPPRRTGHGEQALWAVAPAHRLLPLVDELAARMSVGDGGQ
                     KVEGKRRRGLNRQSDESFTAGLAHAGQYVREHGNLAVQKDTMVGSFRLGEWLHNVQTRA
                     WALSPDRVRVLTALDPWWNVPWSVQWQRSYYRARDHAAVDGWPDAAAGFAGTAVLNGEW
                     LYLQCTQYEALHPEQQRLLADIGVTAEAARTAQPRRASMRARFETGLAHARVYAAKHGH
                     LAVSGKGAVHDGYPLGAWLGAQRSKAQRAARPTERSRALAAVDPWWNPPWPLSWQRSFA
                     QVRWLVQDGHALNALRGFPGLEAESALWLSHQCAVYGSLQRDQQCLLAGIGITAEEAGK
                     VPMTAEEAKAASQAAARLGGSAGLASARSFAAAYRHLALPFDYVHEEYPLGRWLVAQRS
                     KERRHLRTTGTTWPPGLHLTELDPWWNPPWHFPWQRHYQQARTLWQEGRLLLPGQHARP
                     GGRDEDEDPLMAWLRRQCARHDVLLPAQQNLLADIGITAATASTVTASLTTPALGEIGL
                     AQARSYAAEHGNLAVTDRTRHNGQPLGSWLLRQRQRAADGRIEPSRLAALNALDPYWNP
                     SWPLTWQRAYHRARAATDSNALTRTERRWIRTQAQLWDSLHPAQQQLLAALGTAGPAAV
                     PTPVRTARRYPPGEGLPHARAYATLHGHLAVSIHTYQDGFPLGR"
                     /name="helicase associated domain-containing protein"
     gene            6393..6650
                     /locus_tag="P8A18_RS34210"
                     /old_locus_tag="P8A18_34210"
     CDS             6393..6650
                     /locus_tag="P8A18_RS34210"
                     /old_locus_tag="P8A18_34210"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_306061730.1"
                     /translation="MQQRGKARSGQLSAHTLQELTVLDPWWNPPWPYTWHRTWQQYRAT
                     ISRNEPVPDALQRWAAQQRTRRHTLHPHQQRLLTRIGITG"
                     /name="hypothetical protein"
     gene            complement(6736..7566)
                     /locus_tag="P8A18_RS34215"
                     /old_locus_tag="P8A18_34215"
     CDS             complement(6736..7566)
                     /locus_tag="P8A18_RS34215"
                     /old_locus_tag="P8A18_34215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014158402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_306061732.1"
                     /translation="MDAQQLHVVARRVLLDGLTALSDQLDALTVVGAQAVYLRTPEAAL
                     RTSPFTSDGDISIDPDRLLNAPLLDQALQEAGFELLRNNQPGLWARTQQIGDREVPVEF
                     DLLMAKGLAPRIGSRSVKIPPHGVMSARWVPGLEVAAVDRSPMAITSLEASDTRAVTVN
                     VAGPAALLVAKAFKINDRLKQADDRPARLTNKDAGDVLRIIRAIPVAQVAAIFASLSDD
                     PRVGEITREGLQLLQDIFGGPTTPGVDMAVQALSGDLPESTVRALAPAYVSRLT"
                     /name="hypothetical protein"
     gene            complement(7627..8778)
                     /locus_tag="P8A18_RS34220"
                     /old_locus_tag="P8A18_34220"
     CDS             complement(7627..8778)
                     /locus_tag="P8A18_RS34220"
                     /old_locus_tag="P8A18_34220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014158403.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_306061734.1"
                     /translation="MDVDLKSDGEESARQLFDTGLERLRGLFGEGWTVEAAPGHQQDAT
                     DWYTDRVIEIRSTDLGHTTRYVVDLKSSLTPRTVNTELLSKLHLVRQVNSFTSLMVMAP
                     WISPKTQDLLRAHGISYLDLTRNVWIRADRPAIFIYTEGQMRAPRSASPSSSAVTLSGS
                     KAERLVRILADVRPPYRASDLAEATGLSLAYVSRLLGALEDQLLIRRDGRTIHTVNWQG
                     LLRARAEHSHLLNLNPYSGFIAPNGIDYLLNQLRNAFASRHLIAITGSYAAREVAPLSV
                     GGQLMVYIRPDHFSDELVDHLGLLEVKQQADVLFLDAHDPVVFWNSRTVGGLRHVALSQ
                     LALDCLSGPGRLPAEGEAVLERMAAHEQWRNLDIHTIKDWKAA"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(9275..9895)
                     /locus_tag="P8A18_RS34225"
                     /old_locus_tag="P8A18_34225"
     CDS             complement(9275..9895)
                     /locus_tag="P8A18_RS34225"
                     /old_locus_tag="P8A18_34225"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_306061736.1"
                     /translation="MSVEPAHHDGDQPWYEEALVSVAGRLFPPRFRISLRCAATKIEMC
                     TAVEVEQGGFIEPDNATSVAGLSSGVEKDYVRASARKLALPLALATMPDDAFEQSSQGW
                     TWAVAGWSPGSTMRTSPADSRQTALLEELRRRCGPKWWSIYLPRIEKTAEIVWEAPADW
                     PTRRVITALVCPATHVGRTQAYADLQAAREIGLLPPSEDMKAR"
                     /name="hypothetical protein"
     gene            complement(10382..11565)
                     /locus_tag="P8A18_RS34230"
                     /old_locus_tag="P8A18_34230"
     CDS             complement(join(10382..11275,11278..11565))
                     /locus_tag="P8A18_RS34230"
                     /old_locus_tag="P8A18_34230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611786.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_306053333.1"
                     /translation="MPKPYPKEFREDVVRVARNREPGVTLEQIAADFGVHPITLSKWLR
                     RADTDEGGAKPAPVSGESAELREARKRIRLLEQENEVLRRAAAYLSQANLPKMMYPLVR
                     ELAAAAAPDRVPVAVTCRVLNLARQPYYRWLASPVTDTERAEAYRADALFDAHRDDPEF
                     GHRFLADEARAAGEAMAERTAWRICRDNRWWSAFGKRRGRGKNTKAGPPVHDDRVRRDF
                     TAAGPNRLWLTDITEHATGEGKLYLCAVKDVYSSRIVGYSIDARMKSSLAVKALESAVA
                     RRGQVAGCIVHSDRGSQFRSRRFVAVLARHSMIGSMGRVGAAGDNAAMESFFALLQKNV
                     LDRRTWATRQELRIAIVTWIERTYHRRRRQKRLARLTPIEYEAIMTPPAALAA"
                     /name="IS3 family transposase"
ORIGIN
        1 ggtaacgcac aggcttatcc gatctatcgg tccttcgatg tggcgagcgt tacttgtgtg
       61 gcttccgagc tggtccggtg tggcgatcgc caggaaggca agaccggata tttcgatgcg
      121 gagctgcgtg acgtcgccgg tgtgctggcg gcccgggacc tcttcccggg cgcgtggttc
      181 tcccatggcg gctccgactt tcggggcgcc gatctgccca gtgacgttgc agcattggcc
      241 gggctgctgc ccctgcagct tgatgcggag gacctcctgg agggagagac tctctccagg
      301 gctgtgacag tgctgggttc tgcggtgtgg gggcagctcg cgtggatggc gctcacatgg
      361 ccggcggtac cggagctcgg cctcgggccc gagcacgcac gcacgggtgt ccaggcctgc
      421 ttcaacatcg acgcgaacca cgaacccgtg gcgggccaca cggtctacgt ccatgtccat
      481 cccggtgacg aggacagagc acgtcacctt gcccgcaccg tcggacagga cctcatcggc
      541 ccgcccgagt acggctggta aggcccgtca tcattccgga tccggctgaa cagacgaaat
      601 gcgagaaacg ggcatgctga agagctccat ccggcttcga gagcaccagg ttgatcaaag
      661 gaacgccttc cggaagtggg tcggatttcc tgcaagatca tccgtgccac cgcagggcgc
      721 ccggggcacc atcgtgtccg cgaccggctc cggcaagacg atcacagccg ccgcatgcgc
      781 actggagtcg ttcccggacg gccggatcct cgtcaccgtg ccgacgctgg acctgctcgc
      841 gcagaccgca caggcatggc gggcggtggg ccaccgggcg ccgatggtcg cggtgtgctc
      901 cctggagaac gacgcggtcc tcaactcgct gggggtgcgc accactacga acccgattca
      961 gctcgccctg tgggccggga ccgggcccgt ggtcgtgctc gccacgtacg cctccctcgt
     1021 ggaccgcgag gacatcaccg caaccacggg ccaggggaag gtccgcgggc cactggaggc
     1081 cgctctggcg ggcggtgagc ggctctacgg gcagcagatg gacccgttcg acctcacgat
     1141 cgtggacgag gctcatggaa cggctggtga gctggcgcgg ccgtgggcgg cgatccacga
     1201 caacgcccgc atcccggcgg acttccggct gtacctgacc gcgaccccgc gcatcctggc
     1261 cgctccccgc ccgcagaggg gcgcaggcgg ccaggaggcg gagatcgcga gcatggccga
     1321 cgacccggac gggacgtacg gcgcgtggct cgcggagctc gggctgtctg aggcgatcga
     1381 gcgggagatc ctcgccgggt tcgagatcga cgtcctggag atccgcgacc cctcccccgt
     1441 tctcggggag tcggaggagg cgcggcgggg ccggcgcctg gccctgttgc agaccgcgct
     1501 cctggagcac gccgcggcgt acaacctgcg tacggtcatg acgttccacc agaaggtcga
     1561 agaggcccga acgttcgcgg acaagctgcc cgagaccgct gccgcgctgt acctgaacga
     1621 cgcctccgac gccgatctgg ctgccgccga caagctgccg aagtcctcca tcgacgcgga
     1681 gttctacgag ctggaggccg gccgccacgt ccccccggac cgcgtgtggt cggcgtggct
     1741 gtgcggcgac cacctcgtct ccgagcggcg cgaggcactg cgccagttcg ccaacggcat
     1801 cgacgcggcc ggacgccggg tccaccgtgc cttcctcgcc agcgttcgcg tcctcggaga
     1861 gggcgtggac atcaccggcg agcggggagt cgactcgatc tgcttcgcgg acacccgcgg
     1921 ctcccaggta gagatcgtgc agaacatcgg tcgcgcgctc cggctcaaca aggacggcag
     1981 caccaaggtc gccaggatca tcgtgccggt gttcctggag ccgaacgagg acccgaccga
     2041 catggtcgcc agtgcctcgt tccgccccct tgtcgccatc ctccaggggc tccgcagtca
     2101 tgatgatcga cttgtcgagc agctcgcctc ccgtgcgctc acgagcggca agcgcaagat
     2161 ccacgtccgg cgggacgagg acgggcagat cgtcggggcc ggcggcgcgg gcgacggcga
     2221 ggaccaggag gaggaggacg acgacacgca ggccgccgcc gaagcggccc tgctccactt
     2281 ctccagccca cgagacccgg caaccatcgc agccttcctc cgcacccggg tctaccggcc
     2341 cgagtccctg gtgtggctgg agggctacca ggcgctgatc cgctggcgga aggagaacga
     2401 gatcaccggc ctctacgccg ttccctacga catcgagatc gaggtgggag ccacgaaggc
     2461 atttccgctt ggccgatggg tccatcagca gcggaaggcg ctacgggccg gggaactgga
     2521 ggagcggcgc aagaccctgc tggacgcgcc cgaagccggg atggtctggg agccgggcga
     2581 ggaagcctgg gagaacaaac tcgccgcgct ccggtcctac cggcgcgcca tgggacacct
     2641 cgcaccccgt caggacgccg tgtggggcga aggcgagggt gaggcgatgg tgcccatcgg
     2701 gcagcacctc gccaacctcc gccgcaaggg cggcctcggg aaggacgcgg aacgggcccg
     2761 ccggcgcgcg gcgcagctga cggagatcga cccggactgg gactgcccgt ggccgctgga
     2821 ctggcaacgc cactaccgcg tcctcgccga cctcgtcgac gccgacggcc acctgcccga
     2881 catcgcaccc ggcgtggcct tcgacggcga cgatctaggc aaatggctgg cacggcaacg
     2941 caaggcggtc acctgggcgc agctgtccac cgcgcaacag gaacggctgt cccagctggg
     3001 cttgaagccc atccagccgc catctccggc cacagctgcc acccgcgcga cgaagccggg
     3061 caaggcgcag caggcgttcc agcgcggcct gaccgccctc gcccagtacg tcgcacggga
     3121 gggcgagtcc acggtgaagc ggacccaccg agagaagatc gtcatcgacg gccaggagca
     3181 cgatctggcc ttggggatct ggtactccaa ccagaaacaa cgccgggaca aactcaccgc
     3241 cgaacaactc gacgccctcc gggagctggg catggagtgg gcataggagg ccggggtttg
     3301 cagtttttcc gtcgacccgt ggctggtggc cccataacgg gtgaggccga ttgacggtta
     3361 tgggagttgc agatcaacag accgggtggg gtatgggcgg cggaaatcgg aagttggcgc
     3421 cgttagaggc gggggtggtt tccctcgcag gtggggtgtg gcgggtggca ccgctgcaag
     3481 gcgagatgac gcagtcgttt ctgagtcggg tggctgcacg ctatggcgtg aggctcaggg
     3541 atctgctggc ggcaatcgct gaggtaggcg ggctgtcgaa catcaccggt cagacgcggc
     3601 tggacagtga ggtttatctg aaccggcagg ctcgggatcg ggtatctcag ttgtgccgag
     3661 tgccggagcg tcatctgcgg cgggcgctgc ctgcctggga acaggaggag cctcgaaagc
     3721 ggttcgcctc agggccagcg gcgcagtttc atcacactgc ggagaaggtt gtgccctggg
     3781 ggccggcgtg tccggagtgc gcggcgcaat cagccgggcg cgccgagtgc gcgcgcttgt
     3841 atttagaacc gcagcagcgg gtgtgcgcgc tgcaccgtcg gtggctgatg caggtgccgg
     3901 gcacggccgg ccgagtggtg aggttgccga cggggggcga gcagtgggtg caggcccatc
     3961 gtcgtcatgc gaggttgctg cgtcgttcct cggtggctgt ggaggcgttc gaggtggctg
     4021 cagcggtgac tgcctcgtgg tggtggcagg cgtggtcgcg ggaacacgtg tggtcttctc
     4081 ggctgcggag cttgggcagt gggcggatgg atccggaggt gtggcgggtc ctggcgcggg
     4141 agttggttac gtatccggag acggttgcgc tcgccacgct gctggctgat gacgcgttcc
     4201 ggcagtgcct gatcgcggac gcgcggggac atctgccgtt tcggctggct gatttgccag
     4261 tgctgctttc ggcggttgcg cgctgtgtgc ggcgctcctg gtacagggag cagttggcgg
     4321 atgagacgtc ggggccttta ttcgcttggg cgtatcagtg cgtgcggccg ccgcgtcgca
     4381 ccgggcatgg ggagcaggcg ctgtgggcgg tcgctccggc gcaccgtcta cttccgctgg
     4441 ttgacgagct cgccgcgcgg atgtctgtcg gggacggcgg gcagaaggtg gagggcaaga
     4501 gacggcgggg gctcaaccgg cagtcggatg agtccttcac ggccggcttg gcgcatgcgg
     4561 gtcagtacgt gcgcgaacac gggaaccttg ctgttcagaa ggacaccatg gtggggtcct
     4621 tccggcttgg ggaatggctg cacaatgtgc agaccagggc ctgggcgctg tcgccggatc
     4681 gtgtccgggt gctgacggct ttggacccgt ggtggaacgt tccctggtcg gtgcagtggc
     4741 agcgctcgta ctaccgggcg cgcgatcacg ccgccgtgga cggttggccg gatgcagccg
     4801 caggatttgc cgggaccgct gtgctgaacg gtgagtggct gtatctgcag tgcacccagt
     4861 acgaggctct ccatcctgag cagcagcgtc tgctggcgga catcggcgtc acggctgaag
     4921 ccgcccgtac tgctcagccg cggcgggcca gcatgcgggc acgtttcgag acagggttgg
     4981 cgcacgcccg ggtctatgcc gccaagcatg gccacctggc ggtctccggt aaaggggcgg
     5041 tgcacgatgg gtatccgctg ggggcctggc tgggggccca gcgcagtaag gcgcaacggg
     5101 ctgcaaggcc caccgagcgc tcccgcgcgc ttgctgccgt cgacccgtgg tggaatccgc
     5161 cctggccgct gtcatggcag agatccttcg cacaggtccg ttggctggtg caggacggac
     5221 acgcactgaa tgcgctgcgt ggctttccgg gcctcgaagc ggagtccgcg ctgtggctgt
     5281 cgcaccagtg cgccgtgtac ggaagtctgc agcgtgatca gcagtgcctg ctggccggta
     5341 tcggcatcac agctgaggaa gccggcaaag tccccatgac ggcagaggag gccaaggccg
     5401 ccagccaggc cgcagcacgg ctgggtggct cagccggctt agccagcgcc cgttccttcg
     5461 ccgcggcata ccggcacttg gcgctgccct tcgactatgt gcatgaggaa tatccgctgg
     5521 gccgctggct ggtagcacag cgcagcaagg aacgccgcca cctgcggacg acgggaacca
     5581 cctggccgcc cgggctgcac ctgactgagc tggacccctg gtggaacccg ccatggcact
     5641 tcccgtggca gcgccactac caacaggccc gcacgctgtg gcaggagggc aggctgctcc
     5701 tgcccggcca gcacgccagg cccggcggcc gggacgagga tgaagatccg ctgatggcgt
     5761 ggttgcgtcg gcagtgcgcc cggcacgatg tcctgcttcc cgcgcagcag aaccttctcg
     5821 ccgacatcgg tatcaccgct gccaccgcca gcaccgtcac cgcgtccctc accactcccg
     5881 ccctggggga gatcgggctg gcgcaggctc gtagctacgc ggccgagcac gggaacctgg
     5941 ccgtgaccga ccgcacccgc cacaacggcc agcccctggg aagctggcta ctgcgccagc
     6001 gccaacgcgc cgccgacggg cgcatcgagc cctcccgcct cgccgcgctc aacgccctcg
     6061 acccctactg gaacccgtcc tggcccctca cctggcaacg cgcctaccac cgggcccgcg
     6121 ccgccaccga cagcaacgcc ctgaccagga ccgaacgccg ctggatcagg acacaggcac
     6181 agctgtggga cagcctccac cccgcccaac agcaactcct tgccgccctc ggcaccgctg
     6241 gccctgccgc cgtgcccacg ccggtccgga cggcacgccg ctacccgcca ggcgagggcc
     6301 taccccacgc ccgcgcttac gccaccctgc acgggcacct cgcggtatcc atccatacct
     6361 accaggatgg ttttcccctt ggacgctagc tcgtgcagca gcgcggcaaa gcccgctcag
     6421 gccagctgtc cgcccacacc ctgcaagaac tcaccgtcct ggacccgtgg tggaacccac
     6481 cctggcccta cacctggcac cgcacctggc agcaataccg cgccactatc agcaggaacg
     6541 aacccgtccc cgacgcactc caacgctggg ccgcgcagca acgcacccgc cggcacaccc
     6601 tccaccctca ccaacaacgc ctcctcaccc gcatcggcat caccggatga gatcgcggtg
     6661 ttgagcctga aggctctggc ggggacagca acgaagaggt aaggccgcgc cgtcagcgca
     6721 gccggttccg ctgcgtcacg tcaagcggga gacgtaggcc ggcgcgaggg ctcgaactgt
     6781 ggattcgggc aggtcaccgc tgagggcctg tactgccatg tcgacgccgg gtgtggtggg
     6841 tccaccgaaa atgtcttgga ggagctgcag gccttcccgg gtgatctcgc cgactcgggg
     6901 atcgtcgctc aggctggcaa aaatggctgc gacctgagcc acggggatgg ctcggatgat
     6961 gcgcaggacg tcgccggcat ccttgttggt gaggcgtgcg gggcggtcat ccgcttgctt
     7021 caggcggtcg ttgattttga aggccttggc cacaaggagg gcggcggggc cggcgacgtt
     7081 cacggtgacc gctcgggtgt cggacgcctc gaggcttgtg atggccatgg gtgagcggtc
     7141 gacggcagcg acttccaggc ccgggaccca tcgcgctgac atcacaccgt gcggggggat
     7201 cttgacgctg cgactgccga ttctgggggc cagtcccttg gccatgagca ggtcgaactc
     7261 gaccggaacc tcgcggtcgc cgatctgctg agtccgcgcc cacaagccgg gctggttgtt
     7321 acgcagcagc tcgaagcccg cctcctggag cgcttgatcg agaagcgggg cattcagcag
     7381 gcggtcgggg tcaatgctga tgtcgccgtc ggaggtgaag ggagaggtcc gcagggcggc
     7441 ctcgggtgtg cgcaggtata cggcctgcgc gccgacgacg gtcagggcgt cgagctggtc
     7501 gctgagggcg gtcagtccgt ccaggaggac ccggcgcgca actacgtgga gctgctgggc
     7561 atccacgcct tcaggaacta tggccattgt ctccccaccc ttgctgactg cacaggacag
     7621 ctgagcttag gcggccttcc agtccttgat cgtgtgaatg tccaagttcc tccactgttc
     7681 gtgggcggcc attctctcca ggactgcttc gccttcggcc ggtagacgtc cgggaccgct
     7741 gagacagtcg agggcgagct ggctgagtgc cacatgccgc aggccgccga cagtgcggga
     7801 attccagaag accacggggt cgtgggcatc gaggaagagc acgtcagcct gctgcttcac
     7861 ctcaagaagc cccagatggt ccacgagttc gtcggagaag tggtcagggc ggatgtagac
     7921 catgagttgc ccgccgacgg acagcggagc cacctcccgt gcggcgtagg agccggtgat
     7981 ggcgatgagg tggcgggagg cgaaggcgtt gcggagctgg ttgagcaggt agtcgatgcc
     8041 gttgggagcg atgaagccgc tatagggatt caggttgagc aagtgggaat gctctgcccg
     8101 ggcgcgcagc agcccctgcc agttgaccgt gtggatggtg cgaccatcgc gccggatcag
     8161 aagctggtcc tcgagggccc ccagaagccg ggagacatag gcgaggctga gcccggtcgc
     8221 ctcggcaagg tcgctggcac ggtagggcgg ccttacgtcc gccaggatgc gcaccaggcg
     8281 ttcggccttc gagcccgaca aggtcaccgc cgacgaggac ggcgaggcgc tgcgcggggc
     8341 tcgcatctgg ccctcggtgt agatgaaaat ggccggacgg tccgcccgga tccagacgtt
     8401 gcgggtcagg tccaggtagc tgatgccgtg ggcgcgcagg agatcctggg tcttgggaga
     8461 gatccaggga gccatgacca tcagcgaggt gaacgagttc acctgacgga ccaggtgcag
     8521 cttgctcagg agctcggtgt tgacggtgcg aggcgtgagt gaggacttca ggtccaccac
     8581 gtaccgggtg gtgtggccca gatcggtgga gcggatctcg atgacgcggt ccgtgtacca
     8641 gtcagtagca tcctgctgat ggccgggggc cgcctcaacc gtccagccct cgccgaagag
     8701 accgcgcaac cgctccaggc ccgtatcgaa gagttgccta gccgactcct ctccgtcact
     8761 tttcaggtcc acatccactc caatggttac ttaaagtgac aacttgcctg gttggggaac
     8821 tttccttaac taagcaagct ccatttgcag gcaagttaac tctactgctc atgtttcctt
     8881 ggttaagcaa gctcgaagat cgagcaagtt tccctcccgg cggttccccc aaggcggcag
     8941 ccaacgccgc aggcggatcg cattctcaca ggacagaacc gggcgccccg gcgtcccggc
     9001 ccgccgggcg ggatcccacc cggccaacag acgtgatgca ggaccggtgg ccgggccggc
     9061 tgctcgtcgc cctcagcggg cccgcggcgc cggggtgaac atgcctgacg ggccatcgag
     9121 gaacgtaggg gcggcgcagc gctgcgaggg aggattacac cgataggtcg acaccgagct
     9181 agggcagtgg ttcggagagt cgatcgagtt cgttcagcac cgcgttcagt cctcgtctct
     9241 tcccagccgg taagagacgg gcgcccggtg cagctcagcg cgctttcata tcctcggagg
     9301 gtggcaagag tccgatttct cgtgcggcct gcaggtcggc gtacgcctgt gtccgcccta
     9361 cgtgggtggc tgggcagacc aacgccgtga tcacgcgccg ggtaggccag tcggcaggtg
     9421 cctcccaaac gatctctgcc gtcttctcga tccggggtag gtagatggac caccacttgg
     9481 gcccgcagcg gcgccggagc tcctccaaca gtgcggtctg tcggctgtcc gccgggcttg
     9541 tgcgcatcgt gctgcctggc gaccagccag cgactgccca cgtccagccc tgcgatgact
     9601 gttcgaatgc gtcatcgggc atcgttgcga gcgcgagcgg cagcgcgagc ttgcgggcgg
     9661 aggcccggac gtagtccttc tccacgccgg aggagagccc ggcaacggag gtcgcgttgt
     9721 ccggctcgat gaacccgccc tgttcgactt ccaccgcggt gcacatctcg atctttgtgg
     9781 cggcgcaccg caggctgata cggaatcgag gaggaaacag ccgtcccgcc actgatacca
     9841 gtgcttcctc gtaccagggt tgatcaccgt catggtgcgc gggctcaacg ctcatacctc
     9901 cggtccagta cgtgttcacg acgacctcgc accgggccag aggccgccga ccaaaaggcc
     9961 gagcgtcgtg gtgacaccga acgtgcccga agccgtacga accgcgggga caagggggtc
    10021 atgactggcc tgggagagga ccatggagat caacgcggcg acgaggccaa ccacgatggc
    10081 ccccaagatg tagtggagtg gaatcttcac tcggccactc tctcgcccca aagccccata
    10141 aatccacgcc ctccggtccg gggaaccagc ttcgaaaaag cggacaacct gcgagcaggg
    10201 aacacgccca gtttgttcga atccagcggc gatcacctgt cgccctcggg cactggtcag
    10261 acagctgaga cgccaggcac acagcgtatg ccgggctcag gcagcccagc cgatggagga
    10321 gacgttgccg tacgcgtcgt actcggagcg ggactgctgc acgcgtaggt gacagcgggt
    10381 tctatgcagc cagggctgcg ggtggggtca tgatggcttc gtactcgatg ggggtcaatc
    10441 gggccaggcg tttctgacgc cggcgtcggt ggtaggtgcg ctcgatccag gtgacgatgg
    10501 cgatccgcag ttcctggcgg gtggcccagg tgcggcggtc gaggacgttc ttctgcagca
    10561 gcgcgaagaa gctttccatg gccgcgttgt cgccggcggc accgactctg cccatcgatc
    10621 cgatcatgct gtgacgtgcg agaacggcaa cgaacctccg ggaacgaaat tgcgaccctc
    10681 ggtcggaatg cacgatgcat ccagcgacct ggccgcggcg ggcgacggcg gattccaggg
    10741 ctttgacggc aaggctggac ttcatccggg cgtcgatgga gtagcccacg atgcggctgg
    10801 agtacacgtc cttgaccgcg cacaggtaga gcttgccctc gccggtggcg tgctcggtga
    10861 tgtccgtcag ccacagccgg ttcggcccgg cagcagtgaa gtcccgccgg acccggtcgt
    10921 cgtggaccgg cgggccggcc ttggtgttct tgcctcggcc tcggcgtttg ccgaacgcgc
    10981 tccaccagcg gttgtcgcgg cagatccgcc aggcggtccg ctcggccatg gcctcaccgg
    11041 ccgcccgggc ctcgtcggcc aggaaacggt gtccgaactc cgggtcgtcg cgatgcgcgt
    11101 cgaacagggc gtcggcccgg tatgcctcgg ccctctcggt gtcggtgacc ggtgaagcca
    11161 gccaccggta gtagggctgg cgggccagat tgagcacccg gcacgtcacc gccaccggca
    11221 cccggtcagg ggcagcggcg gcggccagct cgcggacgag cgggtacatc attttgacgg
    11281 caggttcgcc tgcgacagat acgccgcagc cctgcgcagt acctcgttct cctgctccag
    11341 gagccggatg cgcttacggg cctcgcgcag ctcggccgac tcacccgaca ccggcgcggg
    11401 cttggcgccg ccctcgtcgg tgtcggcgcg gcgcagccac ttcgacaacg tgatcgggtg
    11461 gacgccgaag tcggcggcga tctgttccag cgtgacgccg ggctcgcggt tgcgtgcgac
    11521 ccgcacgacg tcctcgcgga actctttcgg atacggcttg ggcactgcaa catccttcca
    11581 ggccgcctct cagcaagcca ggtcaggtgt cacctatccg tgcagcagtc ccca
//
