LOCUS       NZ_CP120987             3091 bp    DNA     linear   CON 17-AUG-2023
DEFINITION  Streptomyces sp. Alt3 plasmid unnamed4.
ACCESSION   NZ_CP120987
VERSION     NZ_CP120987.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33694647
            Assembly: GCF_030719215.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. Alt3
  ORGANISM  Streptomyces sp. Alt3
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Widen,T. and Larsbrink,J.
  TITLE     Isolation and description of six Streptomyces strains from soil
            environments, able to metabolize different microbial glucans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Widen,T. and Larsbrink,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAR-2023) DNASense, DNASense ApS, Niels Jernes Vej 10,
            Aalborg 9220, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9.1-b1780
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 64x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/15/2023 08:36:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,716
            CDSs (total)                      :: 7,624
            Genes (coding)                    :: 7,361
            CDSs (with protein)               :: 7,361
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 264
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 264
            Pseudo Genes (frameshifted)       :: 153 of 264
            Pseudo Genes (incomplete)         :: 149 of 264
            Pseudo Genes (internal stop)      :: 16 of 264
            Pseudo Genes (multiple problems)  :: 47 of 264
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP120987.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Streptomyces sp. Alt3"
                     /mol_type="genomic DNA"
                     /strain="Alt3"
                     /isolation_source="soil [ENVO:00001998]|soil organic matter
                     [ENVO:04000008]|plant litter [ENVO:01000628]|plant matter
                     [ENVO:01001121]"
                     /db_xref="taxon:3033808"
                     /plasmid="unnamed4"
                     /geo_loc_name="Sweden: Gothenburg"
                     /lat_lon="57.700650 N 11.963895 E"
                     /collection_date="2022-11-09"
     gene            complement(<1..1004)
                     /gene="mobV"
                     /locus_tag="P8A20_RS38570"
                     /old_locus_tag="P8A20_38570"
     CDS             complement(<1..1004)
                     /gene="mobV"
                     /locus_tag="P8A20_RS38570"
                     /old_locus_tag="P8A20_38570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951564.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_306105481.1"
                     /translation="MLDQIIGFSQSSCYADYQKGGLLVAHVEKYTKGSVQGLSIHWDRK
                     TENHSNQEIDNERSNLNYDLCEKEGDTLSRMNDRLREVHCLNRKDVKVCSDWVVTLPEN
                     LKGTSEKEQREFFEKTYEFLANRYGGEKNVLSANVHKDETTPHMHFAFMPVVWDEKKQR
                     EKVSAKEVLTRKDLKTFHQDLDTFLKKEIPHIYKDGILNDKTIGVDTVKDLKKHSLEIE
                     KQKKAMDAEIKIKEKDLERKIQSLDKEFESKKKKLLDLSEQLPQEIKINAKGKEKKTEV
                     VKKGLFKTETITKNTGNWIIGTNELKRVQKMVNAAYMVKRDYERLQSTDLVEE"
                     /name="mobV"
     gene            2294..2752
                     /locus_tag="P8A20_RS38575"
                     /old_locus_tag="P8A20_38575"
     CDS             2294..2752
                     /locus_tag="P8A20_RS38575"
                     /old_locus_tag="P8A20_38575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125938.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_001125937.1"
                     /translation="MPKQKTENEIIYNGKSRWRNLDTGEIIETDEVIKKTPRNGFMITY
                     LTAIVQLIDSLGNKKMQVVKYILENMDKSTNTLIITTRELTEKSKVSRQTVIDTLKTLE
                     QAQIITRRTGAIMIHPSLVHRGKDTKEKYLLARFEDFNNNKAPINAVE"
                     /name="replication/maintenance protein RepL"
     gene            complement(2782..>3091)
                     /locus_tag="P8A20_RS38580"
                     /old_locus_tag="P8A20_38580"
                     /pseudo=""
     CDS             complement(2782..>3091)
                     /locus_tag="P8A20_RS38580"
                     /old_locus_tag="P8A20_38580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951564.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=2
                     /transl_table=11
                     /product="mob protein"
                     /name="mob protein"
     Typing          2295..2752
                     /name="rep_cluster_1950"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4..1076)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.721"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ttttcttcca ccaaatctgt actttgcaaa cgttcataat cccttttgac catataggca
       61 gcgttcacca ttttttgtac tcttttcaat tcgttcgttc caataatcca attcccagtg
      121 tttttggtta tcgtttctgt tttaaataat ccttttttca caacctctgt ttttttctct
      181 ttcccttttg cattgatttt gatttcttgt ggtaattgtt cactgagatc taaaagcttt
      241 ttctttttac tttcaaattc tttatctaaa gattggattt ttctctccaa gtctttttct
      301 tttatcttta tttccgcatc catcgccttt ttttgtttct ctatttctag agaatgcttt
      361 tttaaatcct taacggtatc aacaccaatc gttttatcat ttaaaattcc atctttgtag
      421 atgtgtggta tttccttttt caaaaatgta tctaaatctt ggtggaatgt ctttaaatct
      481 tttcttgtta aaacctcttt ggctgaaact ttttcccgtt gttttttctc gtcccaaaca
      541 acaggcataa aagcaaaatg catatgaggt gttgtttcgt ccttatgtac gttagcggac
      601 aatacatttt tttcaccgcc ataacgattc gctaaaaact cataggtttt ttcaaaaaat
      661 tcacgttgtt ctttttctga agtccccttc aagttttctg gtaacgtaac aacccaatca
      721 ctacaaactt ttacatcttt ccgattcaaa caatgtactt cacgtaatcg atcattcatg
      781 cgggaaagtg tatctccttc tttttcgcat aaatcataat ttaaatttga ccgctcatta
      841 tcaatctctt gattcgaatg attttctgtt tttctgtccc aatgaatcga taatccttgt
      901 acgctgcctt ttgtatattt ttctacatga gccacaagta agcccccctt ttggtaatca
      961 gcatagcacg aagattgaga gaatccaata atttggtcta gcacgttata ccaaatttca
     1021 tttggacgtg ctctgcgagg ctcgttggct tcgccaaacc atgccttgca tggcaaaaaa
     1081 acctcggact ttgtccgaac ctacaagggg aactcttccc cttgacccca tcaatcgaac
     1141 tccccaaccc ctcaatcctt gagggactcc ctcgccggtt ggcaggccaa aacgtttgac
     1201 gttttgacag gccaagaggg tgaagaatca attgcacgtg tcaattcctt aatgctcctt
     1261 tcatggatca gcacaaaccg ctgacccctt ccagtccgct taccattgac acttaccgct
     1321 aatttttcct tttccaacaa tcgctttgca aattactcca aacatatgtg tttcctattc
     1381 gaatgaatat aagagtaaag tacataataa atatgaaata taccttcaac cttcaactgt
     1441 tacccctaaa aagaacccta tccaacatta gggttctttt ttcataccaa gccaaaaata
     1501 aaaaaggctt atccagttat atacacattt ttgtcccctg catacactac ttatgtacaa
     1561 ctgcctactt gataaattgc catacaacag agtgattgaa aggggatttt atgaattatt
     1621 catttaataa atctgataaa ttaatccctt ttaaaaagaa ctacgggtgt tcaaaaacag
     1681 cctgtaggga cccttgtagt tcaataccaa taaaaaagtc aaaatcctat atagtggatt
     1741 ttgacttttt tattggtatc ccccttgttt ttgggtgtga gtgctggctg ggggtttggg
     1801 ggctagcccc caggaactta acgtaatttt atatggcatt agtgaaattc ttgaacgata
     1861 gatcaaggcg gttcggcagg tacaattgct agtccaagtc gtgcaactta aatcctcgtt
     1921 tttggtatgt tcggcttggc gacatgctca cacctgccga atttcactat gcaatagcaa
     1981 aagagtggag attttttgag tggttttctc aaaaaatact actcgactat tgcattgtgg
     2041 aaagccctct aaaaaaaatg aaaaaagaat atatttaagg cgatatttat cgccttaaat
     2101 atatagaaaa gagtaagcag ggggtaaaaa gtagatttta agctgatata tagctattga
     2161 agggcatttt ctttggtcta attttttata ctttttggtc taatttttta taccttttgg
     2221 tctatttttt tttgaccttt tggtctaatt atgataaaat ggaaatataa aaatatagga
     2281 gaaaggtgat aatatgccaa aacaaaaaac tgaaaacgaa ataatatata acggtaaaag
     2341 tcgatggaga aacctagata caggtgaaat aatagaaact gatgaagtca taaagaaaac
     2401 tcctagaaat ggttttatga tcacatattt aactgcaatc gttcaattaa tagatagttt
     2461 aggtaataaa aaaatgcaag ttgtaaaata catattagaa aacatggaca aatcaacaaa
     2521 tacgttaatt ataactacaa gagaattgac cgaaaaaagc aaggtatcta gacaaactgt
     2581 aatagacaca ttaaaaacac tggagcaagc ccaaataatt actagaagaa ctggagcaat
     2641 aatgatacat ccaagccttg ttcatcgtgg taaagataca aaagagaaat acttacttgc
     2701 tagattcgaa gattttaata ataataaggc tccaattaat gcagttgaat aaaccgccca
     2761 agaattgatt ctaaggcggt tttacctttc catatcatat cctcgttgtt tagtcatttc
     2821 gtttttatgt tcacgctcaa agtaatcctc tacttccctt ccaactagtt cattcttaac
     2881 aagccctcta aacgttttaa attgctcttt aaagactttt ttcgtttgtt gatataaaac
     2941 ctttacgttt atcttcaaat cgtttatatg agcttgtaac gagcctattt ttgtatgtaa
     3001 ttctttattt ctctctctca actccgcatt aatctgatga gatgctttta aattgttaga
     3061 caagccctct acttgcaggt gtaatttttt a
//
