LOCUS       NZ_CP120964             3166 bp    DNA     circular CON 04-APR-2024
DEFINITION  Bacillus cereus strain SCBCM001 plasmid unnamed6, complete sequence.
ACCESSION   NZ_CP120964
VERSION     NZ_CP120964.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33826275
            Assembly: GCF_029590375.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Liu,J., Wu,X., Ding,H., Luo,Y. and Li,Y.
  TITLE     Isolation, Identification and Whole Genome Sequence Analysis of
            Bacillus cereus SCBCM001 Strain from Forest Musk Deer
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Liu,J., Wu,X., Ding,H., Luo,Y. and Li,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAR-2023) College of Veterinary Medicine, Sichuan
            Agricultural University, Huimin Road, Wenjiang District, Chengdu,
            Sichuan 611130, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. v4; CANU v. v1.7.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 168.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029590375.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 07:43:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,881
            CDSs (total)                      :: 5,728
            Genes (coding)                    :: 5,585
            CDSs (with protein)               :: 5,585
            Genes (RNA)                       :: 153
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 106
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 97 of 143
            Pseudo Genes (internal stop)      :: 30 of 143
            Pseudo Genes (multiple problems)  :: 37 of 143
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP120964.1.
            Bacteria and source DNA available from Sichuan Agricultural
            University.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="SCBCM001"
                     /isolation_source="liver"
                     /host="Moschus berezovskii"
                     /db_xref="taxon:1396"
                     /plasmid="unnamed6"
                     /geo_loc_name="China"
                     /lat_lon="30.3 N 103.0 E"
                     /collection_date="2022-10-22"
                     /collected_by="Jie Liu"
     gene            1..1245
                     /gene="mobV"
                     /locus_tag="P4G50_RS29350"
     CDS             1..1245
                     /gene="mobV"
                     /locus_tag="P4G50_RS29350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017562244.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000951644.1"
                     /translation="MAHVQKYAKGNVQGLSIHWDRKTENHSNQDIDNERSHLNYDLCEK
                     EGDTLSRMNDRLSEVHCLNRKDVKVCADWAVTLPESLKDASEKEQREFFEKTYEFLANR
                     YGGEKNVLSANVHNDETRPHMHFAFMPVVWDEKKQQAKVSAKEVLTRKDLKTFHQDLDE
                     FLKKEIPQIYKEGILNDKTIGVDTVKDLKRYSEEIQKQKDVMDAEVKVKERELEGKIQS
                     LDKELESKKNEFLNLSEALPSKINIKVKGKEKKTEVVKTGFLKSETVTTETGNWIVSDS
                     EMRRMQKVLSDANFVKEDYERLQRTDLVKENKELHDRVDSLADGYVKAINENTDLYEKN
                     RELRKEISSLKAHVKDLKENVKVLYHNTKKVLGEHFKAFRGLVKNELDIKGVDNQFDCE
                     YKKEIKKQRGYNMER"
                     /name="mobV"
     gene            complement(1259..1759)
                     /locus_tag="P4G50_RS29355"
     CDS             complement(1259..1759)
                     /locus_tag="P4G50_RS29355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182216.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_001182216.1"
                     /translation="MQPERPLKKVSKVTKNKIIDINTGEIQEEEEIINAYIDKEPDYVK
                     IYLKDIVALNDLPKGLDRVINVLLKVMSYDNMIILNSFIKKQMAQELGYKTVQVLNNNI
                     NKLVNAEILFRKGTGTYQVNARYFGRGHWHDIQEIRLQQVYSTKGRELKTEITYKNDEP
                     SQN"
                     /name="replication/maintenance protein RepL"
     gene            2566..2799
                     /locus_tag="P4G50_RS29360"
     CDS             2566..2799
                     /locus_tag="P4G50_RS29360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="WP_046199669.1"
                     /translation="MTKLEELEKDFNQMNLDLKAIQHDMKSLEVRILVAEKDVLTINKQ
                     LDKISANTTWILRLIISGLLTGVLGVVAKNLL"
                     /name="hemolysin XhlA family protein"
     Typing          2..1242
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggctcacg ttcaaaaata tgcaaaagga aatgttcaag gattgtccat ccactgggac
       61 agaaaaacag aaaatcattc gaatcaagac attgataatg aacggtcaca tttgaattat
      121 gatttatgtg aaaaagaagg agatacactt tcacgcatga atgatcggtt aagtgaagtg
      181 cattgcttga atcgaaaaga tgtaaaagtc tgtgcagatt gggctgttac attaccggaa
      241 agtttgaaag atgcttctga gaaggaacaa cgtgaatttt ttgagaaaac ctatgaattt
      301 ttagcgaacc gttatggtgg tgaaaaaaat gtgttgtcag ccaatgtaca taatgacgaa
      361 acaagaccgc atatgcattt tgcttttatg cctgttgttt gggacgagaa aaagcaacag
      421 gcaaaggttt cagctaaaga agttttaaca agaaaagatt taaaaacatt tcaccaagat
      481 ttagatgagt ttttgaaaaa agaaatacca caaatctaca aagaaggtat tttaaacgat
      541 aaaacgattg gcgttgatac ggttaaagat ttgaaaaggt attctgaaga aatacagaaa
      601 caaaaagatg taatggatgc ggaagtaaag gtcaaagaaa gagagttaga ggggaaaatt
      661 caatcattag ataaagaatt ggaaagtaaa aaaaatgagt ttctgaattt gagtgaagca
      721 ctcccttcaa aaattaatat caaagtaaaa ggaaaagaga aaaaaacaga ggttgtaaaa
      781 acaggttttt taaaatcaga aacagtaact acagaaactg ggaattggat tgtttctgat
      841 agtgaaatga gaaggatgca aaaagttcta agcgatgcaa atttcgtaaa ggaagattat
      901 gagcgtttgc agcgtacaga tttagttaaa gaaaacaaag aattacatga tagagttgat
      961 agtttagccg atggttatgt taaagctatt aatgagaata cagatttgta tgagaagaat
     1021 agagaattac gtaaggaaat aagctcgtta aaagcccatg taaaagattt aaaggaaaat
     1081 gtaaaggttt tataccacaa tacgaaaaaa gtccttggag agcattttaa agcgtttaga
     1141 ggacttgtta agaatgaatt ggatataaaa ggtgtagata atcaatttga ttgtgaatat
     1201 aagaaagaaa ttaagaaaca acgtggatat aacatggaac gttaaaaccg tctcagaatt
     1261 aattctgaga cggttcgtcg tttttgtatg ttatttctgt tttaagctct cttcctttag
     1321 ttgaatagac ttgttgtaat cggatttctt gtatatcgtg ccaatggcca cgaccaaaat
     1381 aacgagcatt tacttgatat gtaccagtac cttttctaaa aagaatttct gcatttacaa
     1441 gtttgttgat gttgttatta agaacttgta ctgttttata tcctagttct tgtgccattt
     1501 gtttttttat aaagctattt agaatgatca tattgtcata agacattact tttaataaaa
     1561 cgttaataac tctatctaaa cctttaggta aatcatttaa ggctacaata tcttttaaat
     1621 atatttttac gtagtctggt tctttgtcga tataagcatt tataatttct tcttcttctt
     1681 gaatttcacc agtatttata tctattattt tattttttgt aactttagaa acttttttca
     1741 agggtctttc cggttgcatc aaatctacct cctacccgat ttataattta attaaattat
     1801 aaatcgggta ttttaaaagt gcaatatttt ttataggatt tcacatttat aatcccacaa
     1861 attataaatg atataaaaaa taaatttaat ttataactcc acaaattacg gtatataact
     1921 ccacaaatta cggttataat cccacaaatt ataacttata atcccacaaa ttatagtatt
     1981 ttcctagaaa tccagtcata tcaacggttc aacctatgtt ctcccttcta ttctattatt
     2041 ttataaaatt taaaagggct tttcactatg caatagtcga gtagtatttt ttgagaaaac
     2101 cattcaaaaa atctccactc ttttgctatt gcatagtgaa attcggcagg tgtgagcatg
     2161 tcgccaagcc gaacagacca aaaacgagga tttagattgt tcgacttgga ctagcaattg
     2221 tacctgccga accgccttgc tctatcgttc taaacaatcc gcttattcca tattcattta
     2281 cgttaagatc cagggggcta gcccccaaac ccccagccag cactcacacc caaaaacaag
     2341 gagagaatga acttagaaca gcacatttat agaaactgtg tttttgctag atgcatatta
     2401 aaaaggataa gcatatgcta tattacgctt tcgtataatt cccttatctt taaagagaac
     2461 ttaaatctta ataatagatt tgagttctct ttttatatca ttattctcta attaccatat
     2521 acatataata tatttaaaat taatttaaaa ggagaagtat gttatatgac aaaattggag
     2581 gaactagaaa aagattttaa tcaaatgaat ctagatttaa aagctataca acatgatatg
     2641 aaaagtttag aagtaagaat attagtagct gaaaaggatg ttttaactat taataaacaa
     2701 ttagataaaa ttagtgcaaa tactacatgg attttacgat tgatcataag cggattgtta
     2761 acaggagtac ttggtgtagt agctaaaaac ctgttatagg aaaaaagggt gttggtcaat
     2821 caaaacggac ctagcaaagc gaagggagta tttgacttga ccaaccgtac tacacccaaa
     2881 ccctcttggc attgaacaaa caatcgtttg ttcctgctaa ccggcgaggg agcccctcaa
     2941 ggattgaggg gttggggagt ttgattgagg gggtctgggg aagagtttcc ccagtgggtt
     3001 tgggcaaagc ccaaggtttg ttttttgcca ggcaaagcat ggcttggcgg agccaaagag
     3061 cctcgcagag cacgtccaaa tgaaatttgg tataacgtgc tagaccaaat ttggtttttc
     3121 ttctatcccc atgttaaact agttaccaaa agggggggtc ttgctc
//
