LOCUS       NZ_CP120961            16013 bp    DNA     circular CON 04-APR-2024
DEFINITION  Bacillus cereus strain SCBCM001 plasmid unnamed3, complete sequence.
ACCESSION   NZ_CP120961
VERSION     NZ_CP120961.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33826275
            Assembly: GCF_029590375.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 16013)
  AUTHORS   Liu,J., Wu,X., Ding,H., Luo,Y. and Li,Y.
  TITLE     Isolation, Identification and Whole Genome Sequence Analysis of
            Bacillus cereus SCBCM001 Strain from Forest Musk Deer
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16013)
  AUTHORS   Liu,J., Wu,X., Ding,H., Luo,Y. and Li,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAR-2023) College of Veterinary Medicine, Sichuan
            Agricultural University, Huimin Road, Wenjiang District, Chengdu,
            Sichuan 611130, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. v4; CANU v. v1.7.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 168.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029590375.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 07:43:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,881
            CDSs (total)                      :: 5,728
            Genes (coding)                    :: 5,585
            CDSs (with protein)               :: 5,585
            Genes (RNA)                       :: 153
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 106
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 59 of 143
            Pseudo Genes (incomplete)         :: 97 of 143
            Pseudo Genes (internal stop)      :: 30 of 143
            Pseudo Genes (multiple problems)  :: 37 of 143
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP120961.1.
            Bacteria and source DNA available from Sichuan Agricultural
            University.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..16013
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="SCBCM001"
                     /isolation_source="liver"
                     /host="Moschus berezovskii"
                     /db_xref="taxon:1396"
                     /plasmid="unnamed3"
                     /geo_loc_name="China"
                     /lat_lon="30.3 N 103.0 E"
                     /collection_date="2022-10-22"
                     /collected_by="Jie Liu"
     gene            1..3243
                     /locus_tag="P4G50_RS29460"
     CDS             1..3243
                     /locus_tag="P4G50_RS29460"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="WP_338405905.1"
                     /translation="MSNNNIPSPFFFNNFNPELIGPTFPPIPPLTLPTGPTGATGPTGS
                     TGATGSTGPTGATGPTGPTGPTGATGSTGPTGATGATGPTGPTGATGATGSTGSTGPTG
                     STGPTGATGPTGATGATGATGATGATGATGPTGSTGSTGATGSTGSTGSTGSTGPTGAT
                     GPTGATGPTGATGSTGATGPTGSTGSTGATGSTGATGATGSTGATGATGSTGPTGSTGA
                     TGSTGATGPTGSTGSTGATGSTGATGATGSTGATGATGSTGPTGSTGATGSTGATGPTG
                     STGSTGATGSTGATGATGSTGATGATGSTGPTGSTGATGSTGATGPTGSTGSTGATGST
                     GSTGATGSTGATGATGSTGPTGSTGATGATGPTGATGPTGATGATGPTGATGSTGATGP
                     TGSTGSTGATGSTGATGATGSTGATGATGSTGATGATGATGSTGATGATGSTGPTGSTG
                     ATGSTGATGPTGATGATGPTGATGSTGATGPTGSTGSTGATGSTGATGATGSTGATGAT
                     GSTGPTGSTGATGSTGATGATGSTGATGATGSTGPTGSTGATGSTGATGPTGSTGSTGA
                     TGSTGSTGATGSTGATGATGSTGPTGSTGATGATGPTGATGSTGPTGSTGATGSTGATG
                     PTGSTGSTGATGSTGSTGATGSTGATGATGSTGPTGSTGATGATGPTGATGPTGATGPT
                     GATGATGSTGATGPTGATGATGSTGATGPTGSTGSTGATGSTGSTGSTGSTGPTGATGP
                     TGATGPTGATGSTGATGPTGSTGSTGATGSTGATGATGSTGATGSTGSTGPTGSTGATG
                     ATGPTGATGSTGPTGSTGATGSTGPTGATGSTGPTGSTGATGSTGATGPTGSTGSTGAT
                     GSTGSTGATGSTGATGATGSTGPTGSTGATGATGPTGATGPTGATGPTGATGATGSTGA
                     TGPTGATGTFSSANASIVTPAPQTVNNLAPIQFTAPVLISKNVTFNGIDTFTIQIPGNY
                     FFIGAVMTSNNQAGPVAVGVGFNGIPVPSLDGANYGTPTGQQVVCFGFSGQIPAGTTIN
                     LYNISDRTISIGGATAARSSIVAARLSFFRIS"
                     /name="collagen-like protein"
     gene            complement(3705..>4180)
                     /locus_tag="P4G50_RS29190"
                     /pseudo=""
     CDS             complement(3705..>4180)
                     /locus_tag="P4G50_RS29190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016136977.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /name="MarR family transcriptional regulator"
     gene            complement(4323..5759)
                     /locus_tag="P4G50_RS29195"
     CDS             complement(4323..5759)
                     /locus_tag="P4G50_RS29195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053955.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="WP_001053964.1"
                     /translation="MNLSIQDELQLFSEELYRHLTPSLLEELAKELGFVKRKRKFSGNE
                     LATICIWVSQRTASDSLVRLCSQLHAATGTLMSPEGLNKRFDKKAVEFLKYIFSALWKS
                     KICETSAISSAALTYFQRIRILDATIFQVPKHLAHVYPGSGGCAQTAGIKIQLEYDLHS
                     GQFLNFQIGPGKNNDKTFGTECLDTLRPSDLCIRDLGYFSLEDLDQMDQRGAYYISRLK
                     LNHTVYVKNPFPEYFRNGTVKKQSQYIQVDLEHIMNNLKPGQTYEIKEAYIGKNQKLFT
                     RVIIYRLTDKQIQERRKKQSYTESKKGITFSEKSKRLTGINIYVSNTPEGVVPMEQIHD
                     FYSLRWQIEIIFKTWKSLFQIHHWQTIKKERLECHVYGRLIAIFICSSTMFKMRKLLLQ
                     KNKRELSEYKAIGMIQDHVSLLYQAIQRNTQNLTKILIRLFDLLQKNGRKSHRYEKKTI
                     FDIMGVVYEYNGFGKQKKAA"
                     /name="IS4 family transposase"
     gene            complement(6112..6558)
                     /locus_tag="P4G50_RS29200"
     CDS             complement(6112..6558)
                     /locus_tag="P4G50_RS29200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620148.1"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_000620148.1"
                     /translation="MIRLEKINADNYQKVFDLELVGEQENFVSSNMRSLAQAWVFYERA
                     KPYAIYNDDTIVGFIMFDYKIENRKVEIWRFMLGSAYQGKGYGKEALNKAIEFLKQENL
                     FDYIQINYVEENLVAKKLYQKVGFSETGEMEGNEVVMRLSIVKE"
                     /name="GNAT family N-acetyltransferase"
     gene            complement(<6861..7070)
                     /locus_tag="P4G50_RS29205"
                     /pseudo=""
     CDS             complement(<6861..7070)
                     /locus_tag="P4G50_RS29205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002188100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /name="DUF421 domain-containing protein"
     gene            complement(7339..7860)
                     /locus_tag="P4G50_RS29210"
     CDS             complement(7339..7860)
                     /locus_tag="P4G50_RS29210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3967 domain-containing protein"
                     /protein_id="WP_000079496.1"
                     /translation="MAQTYWGSEVAKNLGIGSSTLRKYCLALEEVGYPFERGSNNSRIF
                     YHKDVAIIERLLTAMNKKNVTLEQAVNLAMTSVIENEIATVATDSVVDTEQIIVLSERI
                     ERLEQLNLELIQRLDQQSKFLQETDAQRIIREEQRDIQLMQVLREIQDSKRLIAASEQR
                     KSLWSRLFGK"
                     /name="DUF3967 domain-containing protein"
     gene            complement(8041..8652)
                     /locus_tag="P4G50_RS29215"
     CDS             complement(8041..8652)
                     /locus_tag="P4G50_RS29215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002188102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="WP_000558980.1"
                     /translation="MHYIPFMYRYQYPYYVNVPIPMYNYGRQSVYCTFPNEIEHANRFD
                     FFRFSNGDGSISLKDYGSEPFVVNINEAAKQNNTYRTALWTGKHLQVTLMSINVGEDIG
                     LEIHPNVDQFLRIEQGQGIVQMGKSKDNLNFKRNAYDDFAIMIPAGTWHNVINTGNIPL
                     KLYSIYAPPNHSFGTVHVTKADALAVEEDYSHGNRNTVGY"
                     /name="cupin domain-containing protein"
     gene            9257..10207
                     /locus_tag="P4G50_RS29220"
     CDS             9257..10207
                     /locus_tag="P4G50_RS29220"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016107168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="WP_046199663.1"
                     /translation="MPKKVLLFTDLGIDDAFAILYTFFRNDIQLVGIVADYGNVSRENA
                     IKNINYLKYISGRKEIPVFLGASVPLTGILVQYFPEVHGKVGLGPIIPPEIPYPIYPMN
                     DIYKIINSNLDDLTIINLGRLSSLATSFILNLEAMRNVKEYICMGGAFFYPGNVTAVAE
                     ANFYADPYAANLILQHAKNLTIIPLNVTQHAIVTPEMVQQIDAFHRNTQDLAGLIIKPM
                     LDYYYNFYSKSNPGIGGSPMHDFVTIWYLLNPDAVRLSKVPIKVIPDQGEGFGQSIADF
                     RFVTNPGYKTHNIAFQFNYERFKRDIMETFLRKRL"
                     /name="nucleoside hydrolase"
     gene            complement(10888..11550)
                     /locus_tag="P4G50_RS29225"
     CDS             complement(10888..11550)
                     /locus_tag="P4G50_RS29225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002035308.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000162379.1"
                     /translation="MTKKIIDFGQAEKKAKERDSKINSIYEKLEGSGGLSEEERVIMLQ
                     VLSKMSGGEEYFIGKKKKPTDRVRFVQIITDNINYLCKTGYLTNAEKAFLIDLTPYIEF
                     KTNILIECSNEDSEEVDADAATPSYLARKLGKDRSNLSHLMNGLLEKGVLAVAESGMTT
                     EDGRICSSRTWFVNPNVLCCSPKDGVDKATMKIFKKSLRNFKITGDKKKHNLPIYLF"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(11771..13237)
                     /locus_tag="P4G50_RS29230"
     CDS             complement(11771..13237)
                     /locus_tag="P4G50_RS29230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901551.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_000901539.1"
                     /translation="MLGSISFNQSHQSSLSHNNRENIHGNPGIDPARSEENIYFVQKDI
                     RSVYKDVFQEAVDKYNEKQKRNDRKIENYYDKIHKDDKTHEQRELVVAIGEGKDDPKYR
                     EAKKEALKRYAEAFQERNPNLAVYNMVLHDDEANPHLHINYVPNFESSRGLTRRVGMDR
                     ALQQQGVEGTGRKLIAHWRERETAYIEQLAKEYIPNFERANVGSHKYMKVRQYKEYAEA
                     KSTIENQVEEKETKLQTIDDHLKNVEEKTNELEMTKKSLESDVVDTYKELEIVKQQVES
                     ENEKLQLIGQRHVELEKRVEQMQKELDSASDRVPNEPVKIPFLRKEVVVEVQDKMFGKA
                     EITKKQTRNYVLSPEQYQEVTKQVNAAVTIKKDHERLRETDFVKEYESLKMTADGWMKE
                     NRTLKQEKNQLQKEVGVLNREISSLKAHIKGLQTNIRVLYVQTKKVLKEQFKGFRESVK
                     NELGSKGVDNQFEREHKKEVNKHRGFDMER"
                     /name="plasmid recombination protein"
     gene            13869..15305
                     /locus_tag="P4G50_RS29235"
     CDS             13869..15305
                     /locus_tag="P4G50_RS29235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053955.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="WP_001053964.1"
                     /translation="MNLSIQDELQLFSEELYRHLTPSLLEELAKELGFVKRKRKFSGNE
                     LATICIWVSQRTASDSLVRLCSQLHAATGTLMSPEGLNKRFDKKAVEFLKYIFSALWKS
                     KICETSAISSAALTYFQRIRILDATIFQVPKHLAHVYPGSGGCAQTAGIKIQLEYDLHS
                     GQFLNFQIGPGKNNDKTFGTECLDTLRPSDLCIRDLGYFSLEDLDQMDQRGAYYISRLK
                     LNHTVYVKNPFPEYFRNGTVKKQSQYIQVDLEHIMNNLKPGQTYEIKEAYIGKNQKLFT
                     RVIIYRLTDKQIQERRKKQSYTESKKGITFSEKSKRLTGINIYVSNTPEGVVPMEQIHD
                     FYSLRWQIEIIFKTWKSLFQIHHWQTIKKERLECHVYGRLIAIFICSSTMFKMRKLLLQ
                     KNKRELSEYKAIGMIQDHVSLLYQAIQRNTQNLTKILIRLFDLLQKNGRKSHRYEKKTI
                     FDIMGVVYEYNGFGKQKKAA"
                     /name="IS4 family transposase"
     Typing          complement(10889..11550)
                     /name="rep_cluster_1821"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(11775..13237)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.598"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgtcaaata acaacattcc atcaccgttt tttttcaata atttcaatcc agaattaata
       61 ggtccaacat tccctcctat tcctccactt actttaccta ctggacctac tggagctact
      121 ggacctacag gatctacagg agctactgga tctactggac ctactggagc tactggacct
      181 actggaccta ctggacctac aggagctact ggatctactg gacctactgg agctactgga
      241 gctactggac ctactggacc tactggagct acaggagcta ctggatctac tggatctact
      301 ggacctactg gatctactgg acctacagga gctactggac ctactggagc tacaggagct
      361 actggagcta caggagctac aggagctaca ggagctactg gacctactgg atctacagga
      421 tctactggag ctactggatc tacaggatct acaggatcta caggatctac tggacctaca
      481 ggagctactg gacctactgg agctactgga cctacaggag ctacaggatc tactggagct
      541 actggaccta ctggatctac aggatctact ggagctactg gatctacagg agctacagga
      601 gctacaggat ctactggagc tacaggagct actggatcta ctggacctac tggatctaca
      661 ggagctacag gatctactgg agctactgga cctactggat ctacaggatc tactggagct
      721 actggatcta caggagctac aggagctaca ggatctactg gagctacagg agctactgga
      781 tctactggac ctactggatc tacaggagct acaggatcta ctggagctac tggacctact
      841 ggatctacag gatctactgg agctactgga tctacaggag ctacaggagc tacaggatct
      901 actggagcta caggagctac tggatctact ggacctactg gatctacagg agctacagga
      961 tctactggag ctactggacc tactggatct acaggatcta ctggagctac tggatctaca
     1021 ggatctactg gagctactgg atctactgga gctacaggag ctactggatc tactggacct
     1081 actggatcta caggagctac aggagctact ggacctactg gagctactgg acctacagga
     1141 gctacaggag ctactggacc tacaggagct acaggatcta ctggagctac tggacctact
     1201 ggatctacag gatctactgg agctactgga tctacaggag ctacaggagc tacaggatct
     1261 actggagcta caggagctac tggatctaca ggagctacag gagctacagg agctacagga
     1321 tctactggag ctacaggagc tactggatct actggaccta ctggatctac aggagctaca
     1381 ggatctactg gagctactgg acctacagga gctacaggag ctactggacc tacaggagct
     1441 acaggatcta ctggagctac tggacctact ggatctacag gatctactgg agctactgga
     1501 tctacaggag ctacaggagc tacaggatct actggagcta caggagctac tggatctact
     1561 ggacctactg gatctacagg agctactgga tctacaggag ctacaggagc tacaggatct
     1621 actggagcta caggagctac tggatctact ggacctactg gatctacagg agctacagga
     1681 tctactggag ctactggacc tactggatct acaggatcta ctggagctac tggatctaca
     1741 ggatctacag gagctacagg atctactgga gctacaggag ctactggatc tactggacct
     1801 actggatcta caggagctac aggagctact ggacctacag gagctactgg atctactgga
     1861 cctactggat ctacaggagc tacaggatct actggagcta ctggacctac tggatctaca
     1921 ggatctactg gagctactgg atctacagga tctacaggag ctacaggatc tactggagct
     1981 acaggagcta ctggatctac tggacctact ggatctacag gagctacagg agctactgga
     2041 cctactggag ctactggacc tactggagct actggaccta caggagctac aggagctaca
     2101 ggatctactg gagctactgg acctactgga gctacaggag ctacaggatc tactggagct
     2161 actggaccta ctggatctac aggatctact ggagctactg gatctacagg atctacagga
     2221 tctacaggat ctactggacc tacaggagct actggaccta ctggagctac tggacctaca
     2281 ggagctacag gatctactgg agctactgga cctactggat ctacaggatc tactggagct
     2341 actggatcta caggagctac aggagctaca ggatctactg gagctacagg atctactgga
     2401 tctactggac ctactggatc tacaggagct acaggagcta ctggacctac aggagctact
     2461 ggatctactg gacctactgg atctacagga gctacaggat ctactggacc tacaggagct
     2521 actggatcta ctggacctac tggatctaca ggagctacag gatctactgg agctactgga
     2581 cctactggat ctacaggatc tactggagct actggatcta caggatctac aggagctaca
     2641 ggatctactg gagctacagg agctactgga tctactggac ctactggatc tacaggagct
     2701 acaggagcta ctggacctac aggagctact ggacctactg gagctactgg acctacagga
     2761 gctacaggag ctacaggatc tactggagct actggaccta ctggagctac tggaactttt
     2821 tcttctgcta acgctagtat agtaactcct gctcctcaaa ctgttaataa tttagcacct
     2881 attcaattta cagcaccagt tttaataagt aaaaatgtta catttaatgg tattgatact
     2941 tttactattc aaattcctgg taattacttt tttattggtg ctgtaatgac ttcaaataat
     3001 caagctggcc ctgttgctgt tggtgtagga tttaatggta ttccagttcc tagtttagat
     3061 ggagctaatt atggtacacc tacaggtcaa caagttgttt gtttcggatt ttctggtcaa
     3121 ataccagctg gcacaacaat taatttatat aatattagtg atagaactat ttcaataggt
     3181 ggagctacag cagcaagatc aagtattgta gcagctagat tatctttttt tcgcatttct
     3241 taattaatat tattacaaat aatattaatt atataaaatt ttgctttttg gctagagtgc
     3301 tggctcgtcg tgtgggggcg aaaccccacg aattacgtat ctattttaga ataaaaacta
     3361 tttttggacg attgatctaa gcgattcggc ttgtatactt gtttgttcaa gtcgagaacc
     3421 attgttttgc tttttgtctg tcggcttggc gaaaaaatca tacaagccga aaaaagttcc
     3481 gcaaggataa tttttttaaa ggtggagatt tttgagcgtt atttgctcaa aaatactacc
     3541 tgttccttgc ggaactttta gctctatata taagagtgag aagaacaaaa tgaaaaaagt
     3601 taataatatc aagggttcat aagatgttag gtgtcatgtt ttcatgacac ctaacaattg
     3661 ggaaataaca aaaaaaaaaa gaatacaata gaaatttgta ttctttaaaa caaataaaca
     3721 ggaagtttat acttcttttt ttcccttttt cttccaattt aaaattttgt aatgaatctc
     3781 taaaaatatg ttgtgttgct ttgtctatac catcctttgg actacaacac ataatattag
     3841 gatttacaaa ccatgtacga cttgtacaca cccgaccatc ttctgttgtt acccctgctt
     3901 gcgcaacagc taatactcct ttttttaaca aagtattcat tgtacctgaa atattttgac
     3961 gagctatttt aaacttttca ctcaaatatg tgggagatgc tggactacct gtttcacgtt
     4021 ctacaataac attagtctta aattccacaa atgaagctaa tttaaataaa aaagcttctt
     4081 ctttactact caaataacca atctcagtca aataattgat attatccatt attaattgca
     4141 caaacctcac ccgatctaaa ggttttctct tttttcctat aaggggtagc gtcacatgcc
     4201 catcaactta agaacgaaaa caaaatgaac tttcgttcaa acaaactaaa aaacacgtaa
     4261 tgtacttttg gtattgaaaa gtacacacac caaataaact ctaacgagtt tcattttaaa
     4321 aattatgcag ctttcttttg ttttccaaac ccattatact catagacaac acccataata
     4381 tcaaagatag ttttcttctc atatcgatga gatttccgtc cattcttctg taggaggtcg
     4441 aacaaacgga ttaaaatctt tgttagattt tgggtgtttc tctgtatcgc ttgatataac
     4501 agcgatacat gatcttgaat catcccaatg gctttatatt cacttaattc tcttttgttc
     4561 ttttgcaaaa gaagtttacg catcttaaac atcgtagaag aacagataaa gatggcaata
     4621 agtcttccat acacatgaca ttctaatcgc tcttttttga tagtttgcca atgatgaatt
     4681 tgaaataagg atttccacgt tttaaaaata atttctattt gccagcgaag ggagtaaaag
     4741 tcatgtattt gttccatcgg aacaactcct tcaggcgtat ttgagacata tatgttgata
     4801 cccgttaatc gtttactctt ttctgaaaat gtaataccct ttttactttc agtgtaactt
     4861 tgtttttttc tacgttcctg tatctgtttg tctgttaaac gatagataat tacacgtgtg
     4921 aatagttttt gattctttcc aatgtaagct tcttttattt cgtatgtttg accgggtttt
     4981 aaattgttca taatatgttc taaatcaact tggatatact gtgattgctt ttttacggtt
     5041 ccgtttcgaa agtattctgg aaacggattt tttacataca cggtatggtt taattttaat
     5101 ctggatatat aataagcacc tcgttgatcc atttgatcta agtcttctaa tgaaaaataa
     5161 cctaaatcac ggatacataa atcgcttgga cgtaatgtgt ctaagcattc tgttccaaag
     5221 gtcttatcat tattttttcc tggacctatc tgaaagttta gaaactgtcc gctatgtaag
     5281 tcatattcta attgaatctt gatacccgct gtttgtgcac aaccacctga tccaggatat
     5341 acatgggcta aatgttttgg tacttggaaa atcgtcgcat ctaaaatacg aattcgttga
     5401 aaatacgtga gcgccgcgct tgaaatggcc gatgtttcac agattttact tttccataat
     5461 gccgaaaaaa tatatttcaa aaattcaacc gcttttttat caaagcgttt attgagtcct
     5521 tctggactca taagagtgcc tgtggccgca tgtaattgac tgcatagtcg aacaagcgag
     5581 tcactcgctg tacgttgact tacccaaata catatggtag ctaattcatt tccagaaaac
     5641 tttcgttttc tttttacaaa acctaattct tttgcgagtt cttccaaaag agaaggggtt
     5701 aaatgacgat acagttcttc agaaaatagt tgtaattcat cttgaatcga aagattcata
     5761 aaaaatgtca ccctttctag ctagcttttt agaaagaata acgttttttg tcacttgggg
     5821 gtagtgaaaa ttcttaagtt gatgggcatg gggtagcgtc aggaaaatgc gtatttacaa
     5881 cgctaaggaa gttccaatca taaaaaaagc cgatttccag tctctcaagg aatcgacttt
     5941 ttaatttaga ttgttgaact aaagcaccct ttagtttaag tgtaactatt cacaaaacaa
     6001 gaatacaaaa agggctagca gctgccaacc taaaacgttc tatctatttg aaatatactt
     6061 ttagctattc atcactgctg ttaggtaatg aaactggaat atatttgaca cctattcttt
     6121 cactattgag agcctcataa caacttcgtt tccttccatt tctcccgttt ctgaaaaacc
     6181 aactttttga taaagttttt ttgccactaa attttcttca acataattta tttggatata
     6241 gtcgaataaa ttttcttgct tcaaaaattc tatagctttg tttaatgctt ctttaccata
     6301 gccttttcct tgatatgcac tccctaacat aaatcgccaa atctctactt tcctattttc
     6361 tattttatag tcaaacatta taaatcctac aattgtatca tcattataaa tagcataagg
     6421 cttggctctt tcgtaaaata cccaggcttg tgccaacgat ctcatatttg atgaaacaaa
     6481 gttttcctgt tctcctacaa gttctaagtc aaaaactttt tggtagttat cagcgtttat
     6541 tttctctaat ctaatcatct tatacatctc cgagttttgt ttaaatactt gttcttgcga
     6601 taattataac ataatttaaa agactgggca ggggtttata tataacacta aaatttgttg
     6661 aagtcttaat atatatttta aataacttgc ctttcactaa cctgccccat tagtttaagt
     6721 acattttttc acaatctcaa tagagtaagc ctattggtac ataattaaat tatcgttaaa
     6781 ttaggacttc ataatttctc ataagacttg tatcaaaatg cttaacgttg taaatccgca
     6841 tttttctgac gctaccccaa tactaacata agaaaagcaa tgactgcatg atgagttttt
     6901 atatttaaat taaatgccaa attcgcaata atacttccga tagagatagc accaataaaa
     6961 tcaaaagtat tcatttcagc aataatttgc tttcctagta gtttagctcc gataaataaa
     7021 atcgaaaaag ctaatagtgt tctcaaaata atctctatat gttcagacac aagtagctaa
     7081 ccccttttga attaagtgtt cgtattataa caatttatat aaggatagta tgtgtcattg
     7141 taaaagaatt tattatgttc aatagtatta aataattcaa aattaatgtc atggttattc
     7201 ctctgtagct ccccttgaca tcggggagct actcccaatt ttagaaatta taataacacc
     7261 acttataagt aacaaaaaag ctcactcctt atttattttt atataggagt aagcttttct
     7321 ctattattaa attctaattt atttaccaaa tagacgactc caaagcgatt tcctctgttc
     7381 tgaagctgct attaatcgct tactgtcttg tatttctctt aatacttgca ttaattgaat
     7441 atctctttgt tcttctctta ttattctttg agcatctgtt tcctgtaaaa atttactttg
     7501 ttgatccaat ctttgaatta attctaaatt aagctgttct aaacgttcta tacgttctga
     7561 taatactatt atctgttctg tatctactac gctatctgta gcaacagttg ctatctcatt
     7621 ttctataaca cttgtcattg ctagatttac agcctgttct agtgttacgt tcttcttgtt
     7681 catagctgtt aatagccgtt ctataattgc tacatcctta tgataaaaaa ttctagaatt
     7741 attactaccg cgctcgaaag gatacccaac ttcctccaga gcaaggcaat atttacgcaa
     7801 agtacttgag ccaatcccta aattcttggc tacctcagat ccccaataag tttgagccat
     7861 attttgtcct ccctctgctt caagctgttc tccagttgct atatagtctt ttccttcttt
     7921 gaaaaggtaa caccttcttt ttaaaattta aagaagagtt ttcaattgtt ttttaagatt
     7981 aacaaaagtt tatttttttg ctttttaaaa cacagttata gattttataa taaatcttga
     8041 ttaataacca actgtattcc tattgccatg actataatct tcttccacag ccaatgcatc
     8101 tgctttagta acatgaacgg taccaaatga atgatttgga ggagcatata tcgaataaag
     8161 ttttaacgga atattacctg tgttaattac attatgccat gttcctgcag gtatcattat
     8221 tgcaaaatca tcataagcat ttcttttgaa atttaaatta tctttactct ttcccatttg
     8281 aacaatcccc tgaccttgtt caatacgtaa aaattgatca acattaggat gtatttccaa
     8341 accgatatct tcaccaacat taatgctcat taaggtaact tgtaaatgtt tccctgtcca
     8401 taaagcagta cgatacgtat tattttgctt tgctgcttca ttgatattaa caacaaatgg
     8461 ttctgatcca taatctttta acgaaatact tccatcacca ttggaaaaac gaaaaaaatc
     8521 aaatctgttt gcatgctcta tctcattagg aaaagtacaa taaacagact gtcttccata
     8581 gttatacatt ggtattggta cattaacata ataaggatat tgatatcgat acataaaagg
     8641 aatatagtgc atttttctca atccttcaca attttataaa attatcctat gcactggtta
     8701 agtcaatgta cttaaaattc aaagtgactt ttgtaaagaa agatttttag cctaaaacac
     8761 ttatttcatt aaatggacct ttaacttaac aatacaattg aagtttaaaa atcaaaagaa
     8821 aaaaactata tcaacaaagt ttttcatcaa actttgtttt aaaattatca atctttttta
     8881 tacaagattt tatacatttt tttgtactaa tacaggacat tcgtaaaccg gaaaaaccat
     8941 agtatctcaa caatgtataa aatcatgcat aggatgacaa acaaagaaaa agcggaatcc
     9001 ttattatatc aatggattcc gctttttcct tgctgccgat aatgagacgt tatgtcaact
     9061 tcacatgaat atgatataca aaacaaaaat actattttag atgcaaacac atataagata
     9121 tctaaagtta tagatgaaca gaatgtacta ttgaagaata tataaagaat atataaaata
     9181 tatatcaaat atagtacaag cctattcatt acatccttag taaaaataaa attttatggc
     9241 ttttggagga aaatatatgc ccaaaaaagt tctcttgttt acagatttgg gaattgatga
     9301 tgcgtttgct attctgtaca ccttttttcg taatgatatc caacttgtgg ggattgtagc
     9361 tgattatgga aatgtatcga gagagaatgc tataaaaaat attaactatt tgaagtatat
     9421 ttcgggaaga aaagagatac ccgtatttct cggtgcttct gtacctttaa caggtatatt
     9481 ggttcaatat tttcctgaag tacatggaaa agtaggacta ggacctatca ttccacctga
     9541 aatcccttat ccaatttatc ccatgaatga tatttacaaa attataaact caaatttaga
     9601 cgatcttaca attatcaatt tgggaagact ttcctcattg gctacgtcat ttattttgaa
     9661 tttagaagca atgcggaatg tgaaagaata catttgcatg ggtggagcct ttttttaccc
     9721 cggaaacgtg actgctgtgg ctgaagctaa cttttacgca gacccttatg cagcaaactt
     9781 aattctgcag catgcgaaga atttgacaat tattccatta aacgtcaccc aacacgcgat
     9841 tgttacacct gaaatggttc aacaaattga tgcatttcac cggaatacac aagaccttgc
     9901 aggactcatc atcaaaccta tgttagacta ctactataat ttctactcta aatcgaatcc
     9961 tggcatagga ggaagcccta tgcatgattt tgtaacaata tggtatttgt tgaatccaga
    10021 tgcagttcgt ctgtcaaaag tgcccatcaa agtaattccc gatcaaggag aaggatttgg
    10081 tcaaagtatc gcagactttc gttttgtgac aaatccaggt tataaaacac ataatatcgc
    10141 ttttcagttc aattatgaaa gatttaagag agatattatg gaaacatttt taaggaaaag
    10201 actatagcat atcctaagta aagataaaaa tacatattta aaaaagaatt atttatagtt
    10261 ggatgtattg tagttgctat gagtttacta aaatagaact tcctatatcg tgaattatgt
    10321 aaataagccg tccatatgga cggcttattt ttgtttagcc tgattttgtc cttgtttttt
    10381 ggtctacttt ttgctttttg gctagagtgc tggctcgtcg tgtgggggcg aaaccccacg
    10441 tattccgtat caattttgga gtaagcacta ttttaggacg aatgatctag gcggttcggc
    10501 ttgtatactt actagtccaa gtcgatcggc attgctttgc tttttgtctg tcggcttggc
    10561 gagaaaagca tacaagccga aaaaagtttt gcaaggacat aataaaaaag aaaagacggt
    10621 agtctttagg tgaggatgaa cgaaccgact tttttattat gaaggtggag atttttgagc
    10681 gttgtttgct caaaaatact acctgttcct tgcggaactt ttagccctat atataagagt
    10741 gagaagagca aagtaaagaa agtcagtaat atcaagggtt taacaagggt agggtgtggg
    10801 aaaattccca cacatgtgtt gttaaattcc cacacctaaa aaatggaaaa taataaaaaa
    10861 aaaaaagatg tcaataacaa atgacatcta aaataaataa atcggtaagt tatgtttttt
    10921 cttatctcca gttattttaa aatttctaag agacttctta aaaatcttca tagtagcctt
    10981 gtctactcca tctttagggg aacaacataa aacattagga ttcacaaacc aagtacgaga
    11041 actacaaatg cgaccatctt cagttgtcat tccagattct gcaacagcta aaacaccttt
    11101 ttccaataaa ccattcatta aatgagaaag attacttcta tcttttccta acttccttgc
    11161 taaataactt ggtgtagctg catctgcatc tacttcttca gaatcttcat ttgaacattc
    11221 aattaaaata ttagttttaa actctatata cggtgttaaa tcaatcaaaa aagctttttc
    11281 agcatttgtc agatatcccg ttttacatag ataattaata ttatcagtaa ttatctgcac
    11341 aaatctcacc cgatctgtag gttttttctt tttaccaata aaatattctt ctccaccaga
    11401 cattttactt aatacttgta gcataatgac tctttcttct tcagaaagtc cgccactacc
    11461 ttcaagtttt tcataaatac tattaatttt actatctctt tcttttgctt ttttttcagc
    11521 ttgtccaaaa tcaataattt tctttgtcat atctaagtta attcctcctt aattgaggga
    11581 atcaacaaaa atacctattt tttgctattt gattataaat caaattatga taatctataa
    11641 tcagataagt gaagtttagg tattttcgta aaatacccta tgaaaagagt catgttcccg
    11701 catggctctt tttttcattc tatcatgttt ttaacaacct taaagataaa aacaaagttt
    11761 ccctgttttt ttaccgttcc atatcaaatc cacgatgttt attcacctct tttttatgct
    11821 cacgttcaaa ttgattatct actcccttgc tacccaattc atttttaaca ctctctctaa
    11881 accccttaaa ttgctctttt aaaacttttt tcgtttgtac atataaaact cttatattcg
    11941 tctgcaaacc ctttatatga gcttttaacg agctaatttc tctatttaat acaccaactt
    12001 ccttttgcaa ctggtttttt tcctgtttca atgttctatt ttctttcatc caaccgtctg
    12061 cagtcatttt taaactttca tactctttca caaagtctgt ttctctcaaa cgctcatgat
    12121 cctttttgat agtgaccgct gcattcactt gctttgttac ttcttgatat tgttctggtg
    12181 ataacacata atttcttgtc tgtttctttg taatttcagc ttttccaaac attttatcct
    12241 gtacttctac tactacttct ttacgtaaaa atggaatttt aacaggttca tttggcactc
    12301 gatcactagc actatctaat tctttttgca tttgttctac tcttttttct aattccacat
    12361 gacgctggcc aattagctgc agcttttcat tttcactctc tacctgttgc tttactatct
    12421 ctaattcttt gtacgtatca acaacatcac tttccaaact ctttttcgtc atttctaatt
    12481 cgtttgtttt ctcttcaaca ttttttaaat gatcgtcaat cgtttgtaac ttagtttctt
    12541 tttcctctac ttgattttca atagttgact ttgcttctgc atactccttg tattgacgga
    12601 ctttcatgta cttatgtgaa cctacattag cacgttcaaa attcggaata tattctttag
    12661 ccaattgctc tatataagcg gtttcccttt ctctccaatg cgcaattaac tttcttcccg
    12721 ttccttcgac accttgttgt tgcaaagctc tatccattcc gacacgcctc gttaatcctc
    12781 gactactttc aaagttcggt acatagttaa tatgtaaatg tggatttgct tcgtcatcat
    12841 gtaaaaccat gttatagact gccagattgg gattccgctc ttgaaacgct tcagcatacc
    12901 gttttaacgc ttctttctta gcttccctat acttcgggtc gtctttacct tctccaatcg
    12961 ctacgaccaa ttctcgttgc tcatgcgttt tatcatcttt atgtattttg tcatagtaat
    13021 tctcaatctt acggtcattc cgtttttgct tctcgttata cttgtccacc gcttcttgaa
    13081 agacatcctt gtatacactt cggatgtcct tttgaacaaa gtaaatattc tcctctgacc
    13141 tagctggatc aatgccagga ttcccatgaa tattttccct attattgtgg cttaatgaac
    13201 tttggtgaga ttgattaaat gagatagaac ccaacataaa gtgccaccac tttccctaga
    13261 ttaattgata ccataagtct agcaaagcga cgaaatggtg tcaattagag taaccctata
    13321 cttattttga cgctttcgca tcaaaatcga taggggtctg cgacgctttt cggcttcgcc
    13381 gagccatgct tagcatggaa aaaacaacct tggactctgt ccaaacctgc tggggaaatc
    13441 ttccccagac ccccttatcc aactccccaa cccctcaaat ccttgagggc ctccctcgcc
    13501 ggttggcgtc cccaaaacaa tttgttttgg ggaactgcca agaggttgga aaatcagatg
    13561 cacgagtcaa ttccttaatg ctcctttcaa gagatcagca aaaaaacgct gatctctttc
    13621 agtccgctta ccattgactc ttaccactta tttttcttct cccaataatc actttactaa
    13681 ttattcaata ctacacttat taaaaagtaa aaagtttttg agttactttt tttaaacgta
    13741 tttcaaatgc ttttaagttg tattaggggt caccatacat gcccatcaac ttaagaattt
    13801 tcactacccc caagtgacaa aaaacgttat tctttctaaa aagctagcta gaaagggtga
    13861 cattttttat gaatctttcg attcaagatg aattacaact attttctgaa gaactgtatc
    13921 gtcatttaac cccttctctt ttggaagaac tcgcaaaaga attaggtttt gtaaaaagaa
    13981 aacgaaagtt ttctggaaat gaattagcta ccatatgtat ttgggtaagt caacgtacag
    14041 cgagtgactc gcttgttcga ctatgcagtc aattacatgc ggccacaggc actcttatga
    14101 gtccagaagg actcaataaa cgctttgata aaaaagcggt tgaatttttg aaatatattt
    14161 tttcggcatt atggaaaagt aaaatctgtg aaacatcggc catttcaagc gcggcgctca
    14221 cgtattttca acgaattcgt attttagatg cgacgatttt ccaagtacca aaacatttag
    14281 cccatgtata tcctggatca ggtggttgtg cacaaacagc gggtatcaag attcaattag
    14341 aatatgactt acatagcgga cagtttctaa actttcagat aggtccagga aaaaataatg
    14401 ataagacctt tggaacagaa tgcttagaca cattacgtcc aagcgattta tgtatccgtg
    14461 atttaggtta tttttcatta gaagacttag atcaaatgga tcaacgaggt gcttattata
    14521 tatccagatt aaaattaaac cataccgtgt atgtaaaaaa tccgtttcca gaatactttc
    14581 gaaacggaac cgtaaaaaag caatcacagt atatccaagt tgatttagaa catattatga
    14641 acaatttaaa acccggtcaa acatacgaaa taaaagaagc ttacattgga aagaatcaaa
    14701 aactattcac acgtgtaatt atctatcgtt taacagacaa acagatacag gaacgtagaa
    14761 aaaaacaaag ttacactgaa agtaaaaagg gtattacatt ttcagaaaag agtaaacgat
    14821 taacgggtat caacatatat gtctcaaata cgcctgaagg agttgttccg atggaacaaa
    14881 tacatgactt ttactccctt cgctggcaaa tagaaattat ttttaaaacg tggaaatcct
    14941 tatttcaaat tcatcattgg caaactatca aaaaagagcg attagaatgt catgtgtatg
    15001 gaagacttat tgccatcttt atctgttctt ctacgatgtt taagatgcgt aaacttcttt
    15061 tgcaaaagaa caaaagagaa ttaagtgaat ataaagccat tgggatgatt caagatcatg
    15121 tatcgctgtt atatcaagcg atacagagaa acacccaaaa tctaacaaag attttaatcc
    15181 gtttgttcga cctcctacag aagaatggac ggaaatctca tcgatatgag aagaaaacta
    15241 tctttgatat tatgggtgtt gtctatgagt ataatgggtt tggaaaacaa aagaaagctg
    15301 cataattttt aaaatgaaac tcgttagagt ttatttggtg tgtgtacttt tcaataccaa
    15361 aagtacatta cgtgtttttt agtttgtttg aacgaaagtt cattttgttt tcgttcttaa
    15421 gttgatgggc atggggtcac cataccattt taggaaaatt gtacgtttag acacaattcg
    15481 gacacattcc aagcgtttcc tgtacgctaa gagatatctg tccagaaaac cttatattta
    15541 tgaacagagg gtaaaactca acaatatcaa cacgttttct gactataatc atgttcataa
    15601 agcatgtaaa caaatcaatg ttcaattaaa cttaggtttc tatctttctg aacatgtatt
    15661 tacctttaaa gatactgtaa gaataaggtt ttgtagtcct tagcgtacaa ttttcccaaa
    15721 ataatatggt gacccctatt aaaagaaatg aaacatttat ttcagcagac tttataatta
    15781 cactttttaa caatacttaa ttataaaatt caatataaga acttttttta aaacttatac
    15841 atataataaa tattgacata aattcacctg aatagggaaa aaagagatga actggtcctt
    15901 catctctttt tttatgtcaa aaaattaata catttataat aatcaatttt atatctttat
    15961 acatttacta tatataatat atttttttaa taaactagat aggaggtttc tat
//
