LOCUS       NZ_CP120570             6888 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain 582 plasmid p582_6888, complete sequence.
ACCESSION   NZ_CP120570
VERSION     NZ_CP120570.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33748628
            Assembly: GCF_029536655.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6888)
  AUTHORS   Fedrova,P., Hrala,M., Micenkova,L., Tom,N., Bosak,J. and Smajs,D.
  TITLE     Complete genome sequences of five Escherichia coli strains with
            probiotic features
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6888)
  AUTHORS   Fedrova,P., Hrala,M., Micenkova,L., Tom,N., Bosak,J. and Smajs,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Department of Biology, Masaryk University,
            Kamenice 5, Brno 62500, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 395x
            Sequencing Technology :: Illumina; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029536655.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 04:46:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,916
            CDSs (total)                      :: 4,792
            Genes (coding)                    :: 4,563
            CDSs (with protein)               :: 4,563
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 84 of 229
            Pseudo Genes (incomplete)         :: 159 of 229
            Pseudo Genes (internal stop)      :: 42 of 229
            Pseudo Genes (multiple problems)  :: 46 of 229
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP120570.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="582"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p582_6888"
                     /geo_loc_name="Czech Republic"
                     /collection_date="1956"
     gene            complement(join(6739..6888,1..369))
                     /locus_tag="P5711_RS24645"
     CDS             complement(join(6739..6888,1..369))
                     /locus_tag="P5711_RS24645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001318237.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSC
                     GTLWYLGGMIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPHQ
                     GKDWVQLME"
                     /name="MbeB family mobilization protein"
     gene            complement(<407..1105)
                     /locus_tag="P5711_RS24650"
                     /pseudo=""
     CDS             complement(<407..1105)
                     /locus_tag="P5711_RS24650"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1047..1370)
                     /locus_tag="P5711_RS24535"
                     /old_locus_tag="P5711_24535"
     CDS             complement(1047..1370)
                     /locus_tag="P5711_RS24535"
                     /old_locus_tag="P5711_24535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001430281.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1435..1626
                     /locus_tag="P5711_RS24540"
                     /old_locus_tag="P5711_24540"
     CDS             1435..1626
                     /locus_tag="P5711_RS24540"
                     /old_locus_tag="P5711_24540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165988.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDSENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2950..3177
                     /locus_tag="P5711_RS24545"
                     /old_locus_tag="P5711_24545"
     CDS             2950..3177
                     /locus_tag="P5711_RS24545"
                     /old_locus_tag="P5711_24545"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_240079845.1"
                     /translation="MLYAIVISTILCPYSKYAIEHIFFKFIKKDFFKKRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            complement(3174..4739)
                     /locus_tag="P5711_RS24550"
                     /old_locus_tag="P5711_24550"
     CDS             complement(3174..4739)
                     /locus_tag="P5711_RS24550"
                     /old_locus_tag="P5711_24550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]; GO:0031640 - killing of cells of another
                     organism [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_000447003.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEALRH
                     NSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEKEQ
                     AETERQLKLAEDEEKRLAALSEEARAVEVAQKNLAAAQSELAKVDEEINTLNTRLSSSI
                     HARDAETNTLSGKRNELDQASAKYKELDERVKLLSPRANDPLQSRPFFEATRLRARAGD
                     EMEEKQKQVTASETRLNQISSEINGIQEAISQANNKRSTAVSRIHDAEDNLKTAQTNLL
                     NSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAFEKYKDVL
                     NKKFSKADRDAIFNALEAVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTGD
                     WKPLFLTLEKKAVDAGVSYVVVLLFSVLAGTTLGIWGIAIVTGILCAFIDKNKLNTINE
                     VLGI"
                     /name="colicin-like pore-forming protein"
     gene            4999..5436
                     /locus_tag="P5711_RS24555"
                     /old_locus_tag="P5711_24555"
     CDS             4999..5436
                     /locus_tag="P5711_RS24555"
                     /old_locus_tag="P5711_24555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001318234.1"
                     /translation="MGHCNFMAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELIRAYGELKRNETPERHTQGHSEKPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDTERRRQEAERKQLQNEIAQLRQALEQEKKRGFWSRLFGH"
                     /name="hypothetical protein"
     gene            5517..5936
                     /locus_tag="P5711_RS24560"
                     /old_locus_tag="P5711_24560"
     CDS             5517..5936
                     /locus_tag="P5711_RS24560"
                     /old_locus_tag="P5711_24560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(6499..6732)
                     /locus_tag="P5711_RS24565"
                     /old_locus_tag="P5711_24565"
     CDS             complement(6499..6732)
                     /locus_tag="P5711_RS24565"
                     /old_locus_tag="P5711_24565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001555310.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMKLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(1712..1799)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          1810..2368
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.606"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6..1057)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.581"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          complement(6396..6886)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="84.211"
                     /coverage="99.0"
                     /evalue="1.5200000000000001e-56"
ORIGIN
        1 cttcctgata tcttccaggt tcgcctgtat catccctcca agataccaga gcgtcccgca
       61 actcacggta aacaggaaaa tgaccatccc cagtaacatc atgcccgtat tccctgccag
      121 ctttaacacg tccttcctgt gctgcatcat cgcctctttc accccttccc ggtgcttttt
      181 cagtgattcc tctgtcgaag ctgtgaacag ggctatagcg tctttgattt tcgtctcgtt
      241 tgatgtcaca gccttgctta cagatttttc gagcttgctg aactcgtcgt tcagcattgt
      301 ctctgtagat tcggctctct ctttcagctt tttctcgaac tccgcgcccg tctgtaaaag
      361 attgctcata gaatgctcct ttcagtctga tattcttccc gccgttcggg tctgcaatgc
      421 tgatactgct tcgcgtcacc ctgaccactt caatccctgc ttccgtgagc gcctgaatca
      481 catcctgacg gccttttatc tctcctgcat ggtaaagagc atctatcccg cgtgtgacgc
      541 cctcagcaaa cgcctgtttc gtttccggca ggttatcagg gagagtcagc gtccggcggt
      601 tctccggggc gttcgggtca tgcagcccgt aatggtgatt caccagcgtc tgccaggcat
      661 caattctcgg cctgtctgcc cggtcgtagt acggctgtag gcgttttccg gtctccagct
      721 ccatgttcgg aatgacaaaa ttcagctcaa gccgtccctt gtcctggtgc tccacccaca
      781 ggatgctgta ctgatttttt tcgagaccgg gcatcagtac acgctcaaag ctcgccatca
      841 ctttttcacg tcctcccggc ggcagctcct tctccgcgaa cgacagaaca ccggacgtgt
      901 atttcttcgc aaatggcgtg gcatcgatga gttcccggac ttcttccgga ttaccctgaa
      961 gcaccgttgc cccttcccgg ttacgctccc ttcccagcag gtaatcaacc ggaccactgc
     1021 caccaccttt tcccctggca tgaaacttaa ctatcatccc gcgccccctg ttccctgacg
     1081 gccagacgca accggcgcag ctcatccccg atggccatca gtgcggccac cacctgaacc
     1141 cggtcgccgg aagaccactg tccgctgttc accttacggg ctgtctggtt caggttattc
     1201 ccgatggctg ccagctgacg cagtaacggc ggtgccagtg tcggcagcct cccggaacgg
     1261 gcaaccggct cacccaggca gacccgccgc atccataccg ccagttgttt accctcacag
     1321 cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctcgcgt
     1381 ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgact
     1441 aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctg
     1501 ctggagaagc tcaacgaact ggacgcggat gaacaggcag atatctgtga atcgcttcac
     1561 gaccacgccg atgagcttta ccgcagctgc ctcgcgcgct tcggggatga cagtgaaaac
     1621 ctctgacata tgcagctccc ggagacggtc acagcttgcc tgtgagcgga tgccgggagc
     1681 agacaagccc gccagggcgc gtcagggtgt tttagcgggt ttcggggcgc agccctgaac
     1741 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcagcatca gtgcggattg
     1801 tatgaaaagt gcaccatgac cagtgtgaaa taccgcacag atgcgtaagg agaaaatgca
     1861 cgttccggcg cttttccgct tcctcgctca ctgactcgct acgctcggtc gttcggctgc
     1921 ggcgagcggt actgactcac tcaaaaacga taatacagtt atccacagaa tccggggata
     1981 agaccggaaa gaacatgtgc gcaaaaaggc aaagcccaac aaaaggccac gccgatggcg
     2041 tttttccata ggctccgccc ccctgacgag catcacaaaa accgacgctc aagtcagagg
     2101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2161 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     2221 agcgtggcgc tttctcatag ctcacgctgt tggtatctcg gctcggtgta ggtcgttcgc
     2281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2341 aactatcgtc ttgagtccaa cccggtaaga cacgacttag cgccactggc agcagccgtt
     2401 ggtaacttgg ttagcagagc gatgatggcg caaatggtgc aacagagttc ttgaagtagt
     2461 ggcccgacta cggctacact ggaaggacag tatttggtat ctgcgctctg ctgaagccag
     2521 ttacctcggt taagcagttc cccaactgac ttaaccttcg atcaaaccgc ctccccaggc
     2581 ggttttttcg tttacaggca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     2641 ttgatctttt ctactgaaca gagatccccg tcaatttaga agaagatgat ggagccacag
     2701 tccctccctg aacatcacgt atatagttag cctgacatcc aacaaggagg tttatagcga
     2761 atattcccac aaaaaatctt ttcctcataa ctcgatcctt ataaaatgaa aagaatatat
     2821 aacgaggttt aatttatgag tttaagatac tacataaaaa atattttgtt tggcctatac
     2881 tgcgcactta tatatatata ccttataaca aaaaacaacg aagggtatta tttcctagcg
     2941 tcagataaga tgctatacgc aatagtgata agcactattc tatgcccata ttcaaaatat
     3001 gctattgaac acatattttt taagttcata aagaaagatt ttttcaaaaa aagaaaaaac
     3061 ctaaataatg cccccgtggc aaaattaaac ctatttatgc tatacaatct actttgtttg
     3121 gtcctagcaa tcccatttgg attgctagga ctgtttatat caataaagaa taattaaata
     3181 cccaacacct catttatagt attaagttta ttcttatcaa taaaggcaca tagaatgcct
     3241 gtaacaatag caatccccca gatacctaat gtagttccag caagcacact aaaaagtaaa
     3301 acaacaacat aactaactcc agcatctaca gctttcttct ctaatgtaag aaatagcggc
     3361 ttccagtcac ctgtatcctt aatttttagg atatcagata ccacatcata tccaaaagaa
     3421 acatgtcccg taatcttcaa gtacttggca aactgatcta aatgcttagc ccagtcttca
     3481 tacttaaccg cctccagtgc attgaaaatt gcatcacggt ctgctttgct gaatttctta
     3541 tttaatacat ccttgtattt ctcaaaagca gctagagctt cattcacatt gctgattttc
     3601 ttacctttag atttatcagc aagttcctgt gccatttttg aatatttttc accatatttt
     3661 tcagatagcg tttgataaaa gctaactgtt gcatccacag catccttaat ctgcgagttc
     3721 aggagattag tctgtgctgt tttcaaatta tcttcagcat catgaatacg tgaaactgct
     3781 gtacttcgct tattattagc ctgagaaata gcctcttgga ttccatttat ctcagagcta
     3841 atctggttaa gacgcgtttc tgatgctgtt acctgctttt gtttttcctc catctcatca
     3901 ccagctctcg cccgtagtct ggtggcctca aaaaaaggac gactctgaag cggatcattc
     3961 gccctaggag ataaaagttt aaccctttca tccagttctt tatatttagc agatgcctga
     4021 tccaactcat ttcgttttcc ggacagcgta ttcgtttctg catcacgggc atgaatactg
     4081 gagcttaaac gggtattgag cgtattaatc tcttcatcca ctttcgccag ctcagattgt
     4141 gcagcagcaa gatttttttg tgccacctcc acagcccggg cctcttcact caatgctgcc
     4201 aggcgtttct cttcatcctc agccagtttc aattggcgtt ctgtttcagc ctgctccttc
     4261 tctatctctt tacgtcgttg ttctgcttcc tgaaaagcct tttccgcagc ttccgcttct
     4321 ttacgggctt tttcttctgc ttttgcaagg cgcaaccgct ctgcttctgc ctgcatcgct
     4381 gcattattgg catgagccag gtcaataacc tccggatgag tggaattatg gcgaagcgcc
     4441 tcattcacaa tatccttcag atgttgagtc agcgcatccc ggtttgcttt tgctttagcc
     4501 tgtgcttctg cggcagcttt tgctcgggca gcctgttctg cctgcgtttt cttcaattga
     4561 gcagtagacc atttagctgt ggcatgaatg gctgcagaac tttcactttt gctacctcca
     4621 gttccaccac caccgccaga gccactcccg tctggattac cattcaaaag agtaatgatt
     4681 acctgcccct tatcatcata aggaacacca tctttatagt acgctacagc tgtttccatt
     4741 ataaaatcct ctttgacatt aaaaacaaat agttaaaagc aatatactgt atatataatc
     4801 actggtttta tatacagttt aaaattacgc cgctgcattt tccctgtcaa ccctgtggat
     4861 tttcattttt gtgaaaacga tcaaaaaaac agccacccac accacgtgct atttccaccc
     4921 gatgccacaa aaaccagcac aaacattacc gttctcagac ctcattatgt tttactgaaa
     4981 ccatgagatg agacacctat gggacactgt aactttatgg catggcacac actccgggac
     5041 gcactgaaaa tgactggaag atcgcgttca cagttttacc gtgatatgcg ctcaggtctt
     5101 gtcagttacc gtaccagcca ggacggacga cgggagtttg aaaccagtga actgatccgg
     5161 gcatacggcg aattaaagcg gaatgagaca ccagaaaggc acactcaagg acactcagaa
     5221 aaacctcatg atcagcagac agaacgcatt ctccgggaac tgaatgagct gaaacaatgc
     5281 ctgacgctga tgcttgagga taaacaagcc caggatacgg agcgccggcg ccaggaagca
     5341 gaacggaaac agctacagaa tgagatagcc cagctcaggc aggctctgga gcaggaaaag
     5401 aaacggggat tctggtccag gctgttcggt cactgaacgc gctcagaaac tgatgataag
     5461 ataatcttcg gataataact caccgagaat aaatacttta aggtagggag acactcatga
     5521 gacgtaccgg aaacaaactt tgtcttatcg ccatgataac agcaacagta gctctcacag
     5581 cctgtacccc aaagggcagc gtggaacaac atacccggca ttacgtatat gcttctgatg
     5641 acggttttga tcccaacttt tccacccaaa aagccgacac aacacgaatg atggtgcctt
     5701 tttttcggca gttctgggat atgggagcta aagataaagc gacaggaaaa tcacggagtg
     5761 atgtgcaaca acgcattcaa cagtttcaca gccaggaatt tttaaactca ctccggggca
     5821 caactcaatt tgcgggtact gattaccgca gcaaagacct taccccgaaa aaatccaggc
     5881 tgctggctga cacgatttct gcggtttatc tcgatggcta cgagggcaga cagtaagtgg
     5941 atttaccata atcccttaat tgtacgcacc gccgaaacgc gttcggtgcg atcacggcag
     6001 cagacaggta aaaatggcaa caaaccaccc ggaaaactgc cgcgatcgca cccggtaaat
     6061 tttaaccaca tgcataccta tgcagccagg tgaatcacgc aggaattgcc agtcagagac
     6121 cgctgaaacg gatttttttc atactttcgg aagcgaagaa gacggggatt tgagcaggaa
     6181 aacagaagtt caggggaatg agcgcgatct ggcaatagag gcaaaaacac gactatgaaa
     6241 gacacaccag aatcgcgccc gtatgcgttt taacgcgttc ctgtgttttc agcaaggttt
     6301 atgactgaag cgcgttcccg gtgccccgaa ccaaccatta tcccaaaaaa tgcaccggaa
     6361 acgcgctgac aacattttag cctgtctctg attaccatcc cagcgaaggg ccatccagtg
     6421 tccgttcctg tatttccagc tggcgctcac gctccaggga taactcatgt tcgcgttcct
     6481 gtatcagccg ttcgtccctt atctcctgtt ctcgctgtat aactggctca agcgttctgt
     6541 ccgctcgctc aagtgctgca cctgctgact caacttcatg acccgctcgt tcagcatcgc
     6601 gttgtcccgt tgcgtaagcg aaaacatttt ctgcaattcc acgaaggcgc tctcccattc
     6661 gttcagccgc tgcatatagt cctgttgcag ttgctctaat gcgttcagca aatgtctttc
     6721 cagctccgtc actctgtgtc actccatcag ttgtacccag tccttcccct gatggggaat
     6781 cacctctgct gatttcccgt acggaaggac cagaaatttc ctgttcccgt cctgtacgaa
     6841 ctccacgccc catgtcttcg cgttcagttt ctgcaatgtc tcttcctg
//
