LOCUS       NZ_CP120562             2310 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain B771 plasmid pB771_2310, complete sequence.
ACCESSION   NZ_CP120562
VERSION     NZ_CP120562.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33748626
            Assembly: GCF_029536675.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Fedrova,P., Hrala,M., Micenkova,L., Tom,N., Bosak,J. and Smajs,D.
  TITLE     Complete genome sequences of five Escherichia coli strains with
            probiotic features
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Fedrova,P., Hrala,M., Micenkova,L., Tom,N., Bosak,J. and Smajs,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Department of Biology, Masaryk University,
            Kamenice 5, Brno 62500, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 270x
            Sequencing Technology :: Illumina; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029536675.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 04:47:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,259
            CDSs (total)                      :: 5,145
            Genes (coding)                    :: 4,838
            CDSs (with protein)               :: 4,838
            Genes (RNA)                       :: 114
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 307
            CDSs (without protein)            :: 307
            Pseudo Genes (ambiguous residues) :: 1 of 307
            Pseudo Genes (frameshifted)       :: 123 of 307
            Pseudo Genes (incomplete)         :: 222 of 307
            Pseudo Genes (internal stop)      :: 67 of 307
            Pseudo Genes (multiple problems)  :: 87 of 307
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP120562.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="B771"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pB771_2310"
                     /geo_loc_name="Czech Republic"
                     /collection_date="2009"
     gene            1240..1737
                     /locus_tag="P5709_RS26230"
                     /old_locus_tag="P5709_26230"
     CDS             1240..1737
                     /locus_tag="P5709_RS26230"
                     /old_locus_tag="P5709_26230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027759.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_001027759.1"
                     /translation="MNITERTDSKKKVLRQNITEVIDRETGNITQEITDITVQFPQEPA
                     YVKIYIDNLCAVTKAPDSLKDVLFLILRKLDYDGYIALSTRYRKEICKLLGIKDGTLRN
                     RLYSLSKMGIIASCGGNEYQANPNLFARGEWKKIIEQRREFEMRIKYSPNGETIITTEE
                     IK"
                     /name="replication/maintenance protein RepL"
     gene            complement(1771..2196)
                     /gene="mobA"
                     /locus_tag="P5709_RS26235"
                     /old_locus_tag="P5709_26235"
     CDS             complement(1771..2196)
                     /gene="mobA"
                     /locus_tag="P5709_RS26235"
                     /old_locus_tag="P5709_26235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107325.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_001107325.1"
                     /translation="MAKNKRSGSEVRQRTKVITLRVNNRVASEIRRRAKNEGLTISEYI
                     RTASLNNEIKQRVPSRYLDELIRLGRMQKKLFDKGKRPKDKEYLEVMHKIILLCDEMKI
                     VTKRISDVYNEMDLIKDEIKIIKRIHKNKYPGSDLFK"
                     /name="mobA"
     Typing          complement(633..891)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.415"
                     /coverage="100.0"
                     /evalue="6.900000000000001e-74"
ORIGIN
        1 ccgctacaac ttttcgacga aaataaaata ccgcgtcgta acctggtatt tctgatacaa
       61 aaaaatgatg cttcattcaa tattatttac acagatttta aaaattattg cttgttattt
      121 aataaataac atcgaattaa ttgatgtctt tgttgtctgt cgtttgtgga ttattggagg
      181 gataaagatt agatcttcct cgttatataa tccattgcct gttaatgata cggagtgtgt
      241 ccaaggaaca ggaagcggaa gagcaagata gtagcatgta cataaaatat aaatccgtga
      301 gttattgata tcgcgtttat atattaaaaa tataacttaa cattgctctt aatatttatt
      361 ttgtcctggc aatgggagca ttaatccccg tgcagtgctc cgccagctgc tgacaacctc
      421 gacatataac catcgatacg atagaacatc agggcgcgtt tccggcaaga actaaccatg
      481 gggatggttt gcgtgccgac aaacgccgga acgcgcctca aatccaccaa gccaccacgg
      541 ttccggcagc gccttgagcg caaccggaaa ccgtctgggg agaaccccag acccccaccg
      601 ctgaaacatc atcggtctac gaccacctac caaccgagtg gctacctgtg ccgtgcgaac
      661 aggctttgca gggggctggc gcggttcctg gcgacctgaa agcccctggt taggggtatc
      721 ggcgcgcctg gcctcctgaa accctctgta agcgtttttc gcgcctttca tgccctgacg
      781 ggcatttaaa ccccgtttcg tgcgtctggg aggctctgag cgcgtttaac agatgaccag
      841 cgactcgatc gcctgagatc aaatcgggat catcaacgat atgcgagagg tggcaccgct
      901 cgccgcagcc ccagcgaccg aacgtagtga gcgagtgggc gaggaagcgg aagagaaccg
      961 gaagctacat tgagcacgca cgctcaatat taaaattgtc acttcgtaat atagagatta
     1021 tgcctcgtgc ttatggatac atttaaaaat ataagatatc cgaattaggc tacatacggg
     1081 ctgatcgcca atgttgtaac gtgtagaaga tacacctgta acgtgtacag gatactcctg
     1141 taacgtgtgg gcggtattgt cgtaatctat attgagtgac attttgtttt tttggcatta
     1201 tattgtctgt cacctttata aagtgacaaa ggcgataaaa tgaacattac tgaaagaacg
     1261 gatagcaaga aaaaagtttt aagacaaaat attactgagg ttatagatag agaaactgga
     1321 aacataacac aagaaataac cgatataaca gttcaatttc cacaagaacc tgcgtatgtg
     1381 aaaatttata ttgataattt atgtgctgtc acaaaagcac ctgattctct aaaagatgtt
     1441 ttatttttaa ttcttaggaa attggattat gacggatata ttgcattatc aactagatac
     1501 cgtaaggaga tctgtaaatt attgggaata aaagacggaa cattacgtaa cagattgtat
     1561 tcattatcaa aaatggggat tattgctagt tgtggcggta atgaatatca ggcgaaccct
     1621 aatttatttg ctcgtggtga atggaaaaaa ataattgaac agcgtcgcga gtttgaaatg
     1681 agaattaaat attcacctaa cggtgaaact attataacaa ccgaagaaat aaaataatgt
     1741 gcacctaaat aaggtctttt taaaaagacc ttatttgaat aaatccgaac ctggatattt
     1801 atttttatgt atccttttta ttatttttat ttcatccttt attaaatcca tttcattata
     1861 tacatcagaa attcgcttcg ttactatttt catttcatcg caaagtaaaa ttattttatg
     1921 catcacctcg aggtactcct tatccttcgg ccgctttccc ttatcaaata atttcttctg
     1981 catccgacca agtcggatta attcatctaa atacctggaa ggtacgcgct gttttatttc
     2041 attatttaaa cttgccgtcc tgatatattc tgaaattgtc agtccttcgt tcttcgctcg
     2101 ccgtctgatt tcgcttgcga ctctgttatt tactctaagg gtgataacct tagttctctg
     2161 ccgtacttct gaaccactac gtttattttt agccatcgca atgactctcc attttgcacg
     2221 tttggggtgt cggggcttgc cctgacagga ggtattctca tgcaagcgaa tgtattcgcg
     2281 gtatgacgaa tacacatcct gtccaatttt
//
