LOCUS       NZ_CP120561             2328 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain B771 plasmid pB771_2328, complete sequence.
ACCESSION   NZ_CP120561
VERSION     NZ_CP120561.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33748626
            Assembly: GCF_029536675.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Fedrova,P., Hrala,M., Micenkova,L., Tom,N., Bosak,J. and Smajs,D.
  TITLE     Complete genome sequences of five Escherichia coli strains with
            probiotic features
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Fedrova,P., Hrala,M., Micenkova,L., Tom,N., Bosak,J. and Smajs,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Department of Biology, Masaryk University,
            Kamenice 5, Brno 62500, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 270x
            Sequencing Technology :: Illumina; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029536675.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 04:47:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,259
            CDSs (total)                      :: 5,145
            Genes (coding)                    :: 4,838
            CDSs (with protein)               :: 4,838
            Genes (RNA)                       :: 114
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 307
            CDSs (without protein)            :: 307
            Pseudo Genes (ambiguous residues) :: 1 of 307
            Pseudo Genes (frameshifted)       :: 123 of 307
            Pseudo Genes (incomplete)         :: 222 of 307
            Pseudo Genes (internal stop)      :: 67 of 307
            Pseudo Genes (multiple problems)  :: 87 of 307
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP120561.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="B771"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pB771_2328"
                     /geo_loc_name="Czech Republic"
                     /collection_date="2009"
     gene            182..607
                     /gene="mobA"
                     /locus_tag="P5709_RS26220"
                     /old_locus_tag="P5709_26220"
     CDS             182..607
                     /gene="mobA"
                     /locus_tag="P5709_RS26220"
                     /old_locus_tag="P5709_26220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107325.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_072652821.1"
                     /translation="MVKNKRNGSETRQRSKVITLRINNKIDREIRRLAKAEGMTISEYI
                     RTSCLNKEIIQRIPSRYLDELLELGRMQKNLFDKGRTPKDKDYLDVMHKIITLCAEAKE
                     VILRNSDIYKEMDLLKQEIKIIKQYYKNKYPGSDLFN"
                     /name="mobA"
     gene            complement(649..1176)
                     /locus_tag="P5709_RS26225"
                     /old_locus_tag="P5709_26225"
     CDS             complement(649..1176)
                     /locus_tag="P5709_RS26225"
                     /old_locus_tag="P5709_26225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279566.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_072650454.1"
                     /translation="MSVERYKSNPFLENMIVPVKGRQVKLSRLGKDNNILMNQSTGEFL
                     GTHVTTYKQVDADQFIKIFTDNIAMTFDFTSAGIKAFGVLLWAVQHHALSKDEVDLDSF
                     ILDAFISENNKENKKIRLSMATFKRGINELEKASIIAKTLRKGRYYINPNFVFNGDRIA
                     FTNLIERKAKDK"
                     /name="replication/maintenance protein RepL"
     Typing          1532..1764
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.362"
                     /coverage="89.0"
                     /evalue="3.19e-77"
ORIGIN
        1 tcattttttg tatcagaaat accaggttac gacgcggtat tttattttcg tcgaaaagtt
       61 gtagcggaaa atgggacagg atgtgtattt gtcataccgc gaatacattc gcttgtatga
      121 aaatacctcc tgtcagggca ggccccgaca ccccgaacgt ggaaaccaga ggttcattgt
      181 gatggttaaa aataagcgca acggttcaga aacacggcag agaagcaagg ttataacctt
      241 gcggataaat aacaagattg acagggagat ccgacgcctg gcaaaggcgg aagggatgac
      301 gatttcagaa tatataagaa cgtcatgttt aaataaagaa ataatacagc gcatcccttc
      361 cagatatttg gatgaattat tagagcttgg ccggatgcag aagaatttat ttgataaagg
      421 aagaacaccg aaggataagg actaccttga tgtgatgcac aaaataatca cgctgtgtgc
      481 agaagcgaag gaggttattt tacgtaattc tgatatatat aaagagatgg atttattaaa
      541 acaggaaata aaaataatta aacagtacta caaaaataaa tacccaggct ctgatttatt
      601 taattaaaat aaaccctgct caatttttag agcggggttt atatgttgtt atttgtcttt
      661 tgcttttctt tctatcagat ttgtgaaggc tattctatca ccattaaaaa caaagtttgg
      721 gtttatataa taacgtcctt ttcttagggt ttttgctatt atacttgctt tctcaagttc
      781 attaatgccc cttttaaatg tggccatact caaccttatc ttcttgtttt ctttattatt
      841 ttcagagatg aaggcatcca atataaaact atctaaatca acttcatctt tagacaatgc
      901 atgatgctga accgcccaaa gaaggacacc aaaagcttta attccggcgg aagtaaaatc
      961 aaatgtcatc gctatattat ctgtaaatat tttgataaat tgatcggcgt cgacttgttt
     1021 atatgttgtc acgtgagtac caaggaactc tcccgtcgat tgattcatta atatattatt
     1081 atctttgcct aaacgagata atttaacctg cctacctttg acaggcacga tcatattttc
     1141 caaaaatggg ttcgatttgt acctttcaac gctcataatt caccattata ttgaacctta
     1201 gtagctaaaa aagcgccctt tcaaagctca taatgaacga actatttgag ctttgaaagc
     1261 tcacactata gctaacacgg ctcaaaaaca acacctattt tgagccgtgt tggtaacagg
     1321 gttgagctaa caagttacag atccgaagat cacgatcccc atttcatgcg ggttgcagag
     1381 gattcccttc ttagttctta tacggtggtg cgtaagtgcc ctaggctgac atctggttca
     1441 cttccgcttc ctcgcccact cgctcactaa cgttcggtcg ctggggctgc ggcgagcgga
     1501 tcctcctctc tcgtagtggc tggcttttgg tgttgttctt tcgaagatga gtcgtcagca
     1561 tggccctaaa acgcgctcag agcctctcaa acgcacgaaa cggggtttaa atgcccgtga
     1621 gggcatggaa ggcgcgaaaa acgcttacag agggtttcag gaggccagac gcgccgatac
     1681 ccctaaccag gggctttcag gtcgctagga accgcgccag ccccctgcaa agcctgtttg
     1741 cacggcacag gtagccactc ggttggtagg tggtcgtaga ccgatgatgt tgcagcggtg
     1801 ggggtctggg gttctcccca gtcggtttcc ggttgcgctc aaggcgctgc cggaaccgtg
     1861 gcgactcggt aggtttgagg cgcatttccg gcgttcgttg gcacgcaaac catccccatg
     1921 gttagttctt gccggaaacg cgccctgatg ttctatcgta tcgatggtta tatgtcgagg
     1981 ttgtcagcag ctggcggagc actgcacgag gattaatgct cccattgcca ggacaaaata
     2041 aatattaaga gcaatgttaa gttatatttt taatatataa acgcgatatc aataactcac
     2101 ggatttatat tttatgcaca tgctactatc ttgctcttcc gcttcctgtt ccttggacac
     2161 actccgtatc actaacaggc aatggattat ataacaagga agatctaatc tttattcctc
     2221 caataatcca caaacgacag acaacaaaga tataaattaa tttggtgtta tttattaaat
     2281 aacaagcaat aatttttaaa atctgtgtaa ataatattga atgaagca
//
