LOCUS       NZ_CP120560             9494 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain B771 plasmid pB771_9494, complete sequence.
ACCESSION   NZ_CP120560
VERSION     NZ_CP120560.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33748626
            Assembly: GCF_029536675.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9494)
  AUTHORS   Fedrova,P., Hrala,M., Micenkova,L., Tom,N., Bosak,J. and Smajs,D.
  TITLE     Complete genome sequences of five Escherichia coli strains with
            probiotic features
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9494)
  AUTHORS   Fedrova,P., Hrala,M., Micenkova,L., Tom,N., Bosak,J. and Smajs,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Department of Biology, Masaryk University,
            Kamenice 5, Brno 62500, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 270x
            Sequencing Technology :: Illumina; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029536675.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 04:47:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,259
            CDSs (total)                      :: 5,145
            Genes (coding)                    :: 4,838
            CDSs (with protein)               :: 4,838
            Genes (RNA)                       :: 114
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 307
            CDSs (without protein)            :: 307
            Pseudo Genes (ambiguous residues) :: 1 of 307
            Pseudo Genes (frameshifted)       :: 123 of 307
            Pseudo Genes (incomplete)         :: 222 of 307
            Pseudo Genes (internal stop)      :: 67 of 307
            Pseudo Genes (multiple problems)  :: 87 of 307
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP120560.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="B771"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pB771_9494"
                     /geo_loc_name="Czech Republic"
                     /collection_date="2009"
     gene            complement(1594..1827)
                     /locus_tag="P5709_RS26165"
                     /old_locus_tag="P5709_26165"
     CDS             complement(1594..1827)
                     /locus_tag="P5709_RS26165"
                     /old_locus_tag="P5709_26165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_087599083.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTEHLSQLYSESRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1834..2352)
                     /locus_tag="P5709_RS26170"
                     /old_locus_tag="P5709_26170"
     CDS             complement(1834..2352)
                     /locus_tag="P5709_RS26170"
                     /old_locus_tag="P5709_26170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001749510.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQTNLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2390..3088)
                     /locus_tag="P5709_RS26335"
                     /pseudo=""
     CDS             complement(<2390..3088)
                     /locus_tag="P5709_RS26335"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3030..3353)
                     /locus_tag="P5709_RS26180"
                     /old_locus_tag="P5709_26180"
     CDS             complement(3030..3353)
                     /locus_tag="P5709_RS26180"
                     /old_locus_tag="P5709_26180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_087599052.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSCDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3418..3609
                     /locus_tag="P5709_RS26185"
                     /old_locus_tag="P5709_26185"
     CDS             3418..3609
                     /locus_tag="P5709_RS26185"
                     /old_locus_tag="P5709_26185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165974.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3761..3979)
                     /locus_tag="P5709_RS26340"
     CDS             complement(3761..3979)
                     /locus_tag="P5709_RS26340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_072021258.1"
                     /translation="MFFPALSPDSVDNRITAFECAGTARRSRTTERSESVSEEAEECRK
                     LHFLLTHLCGISHRLWCTFHTIRTDAA"
                     /name="hypothetical protein"
     gene            4279..4437
                     /locus_tag="P5709_RS26345"
     CDS             4279..4437
                     /locus_tag="P5709_RS26345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(4634..4774)
                     /gene="crl"
                     /locus_tag="P5709_RS26190"
                     /old_locus_tag="P5709_26190"
     CDS             complement(4634..4774)
                     /gene="crl"
                     /locus_tag="P5709_RS26190"
                     /old_locus_tag="P5709_26190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin R system lysis protein"
                     /protein_id="WP_010892547.1"
                     /translation="MKMILSGGMLVLMALLSGCQANYIRDVQGGTVAPSSSSKLTGIAV
                     Q"
                     /name="crl"
     gene            4894..5418
                     /gene="cui"
                     /locus_tag="P5709_RS26195"
                     /old_locus_tag="P5709_26195"
     CDS             4894..5418
                     /gene="cui"
                     /locus_tag="P5709_RS26195"
                     /old_locus_tag="P5709_26195"
                     /inference="COORDINATES: protein motif:HMM:NF015795.4"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity protein Cui"
                     /protein_id="WP_021534853.1"
                     /translation="MRKEHTDNKDLNRTLFLITFAGITPLIIIFITYTSNPKFYLISII
                     FDNTQNIPSIISAYNPVMTKVMDIYGKSAPLLALIAFTLQLRDRKLETIANREKLITAS
                     IFSPFFYAFYAYFFLWNNFELTTAGRTVRWMSDNDFTLFIFYACLYFCSFFMTYALCYI
                     PVVSYKLWKER"
                     /name="cui"
     gene            complement(5446..7335)
                     /gene="cya"
                     /locus_tag="P5709_RS26200"
                     /old_locus_tag="P5709_26200"
     CDS             complement(5446..7335)
                     /gene="cya"
                     /locus_tag="P5709_RS26200"
                     /old_locus_tag="P5709_26200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010892543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin Y"
                     /protein_id="WP_109336664.1"
                     /translation="MVCFKSQRGMNMPGFNYGGYGDGTGWSSESGGPAPGGGMHGNSGG
                     QRGDNANSSNSVSQQISAIQNDQKLKQKVVNMLIAARKMNPEAKMILGSIAPSGVMQVT
                     IEGITSTQAKQLGLGGLVMGYNASGVIGAVGEIDTGHRLNASGASTPGSETSVESFVNG
                     QKPAGEWHAVAKDSWTGAGPVNVGLVNNAIKSVRIIKKGYVTGVLLPEEVMNKAEYKAM
                     RQAFDSLPLAKQGEAVRQIVAAWSLAYQDFPVNLKKDMGRVTERVVDAVNLALILNQIS
                     SGMSASQKDVDAANRIINETVKAINDVNLKIAEKKKQQVPLLSLMKQKQKEVEELKKVF
                     KNHSYHRIRDAQRAYDDARNKNDLLVSDINALQAQVSGLTARKQQAEKNKAAAEKAKAD
                     AKAKAEAEKAAAEAKAKAEAEKARKESEAKANNEKAVLTKASEIIISVGDKVGEYLGDK
                     YKALSREIAGNIKNFQGKTIRSYDEAMASVNKLMANPDLKINAADRDVIVNAWKAFDAE
                     DMGNKFAALGKTFKAADYVMKANNVREKSIEGYQTGNWGPLMREVESWVVSGIASAVAL
                     AIFSATLGAYLLAVGASAAVVGIIGIIIASFIGALIDDKFIDRLNNEIIRPAY"
                     /name="cya"
     gene            7560..7997
                     /locus_tag="P5709_RS26205"
                     /old_locus_tag="P5709_26205"
     CDS             7560..7997
                     /locus_tag="P5709_RS26205"
                     /old_locus_tag="P5709_26205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WP_170973632.1"
                     /translation="MGHCNFMAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELLRAYGELKQNETQERHTVGQAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRHQESEREQLQNEIAQLRQALEQEKKRGFWSRLFGH"
                     /name="entry exclusion protein 1"
     gene            8078..8506
                     /locus_tag="P5709_RS26210"
                     /old_locus_tag="P5709_26210"
     CDS             8078..8506
                     /locus_tag="P5709_RS26210"
                     /old_locus_tag="P5709_26210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_000830435.1"
                     /translation="MKRSGGTRYLYLISLITVAAALTACTPKGSVEQHTRHYVYASDDR
                     SDPNFYTNKADTTRMMIPFFQQFREMGEKDKAAGVSAETAQQRIKEFHSEKFLQSLRST
                     TTFAGRKYTNSDMPSPEKMKLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            9032..>9208
                     /locus_tag="P5709_RS26215"
                     /old_locus_tag="P5709_26215"
                     /pseudo=""
     CDS             9032..>9208
                     /locus_tag="P5709_RS26215"
                     /old_locus_tag="P5709_26215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611469.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     Typing          complement(3695..3782)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          3805..4351
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.536"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(1491..3040)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.702"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acacgctctg ttttttaatt tacacaagaa tatatgtctg gaaaatcagg aagaagaaat
       61 taatgggcat tctgtatacg aagggagagc gcgtttccgg tgccccatat tagatattat
      121 ccctgcaaca aacaccagag acgcgctcat aattcttcta gcgtaaatga tcctttcagg
      181 caagaaaaaa gccgtgattc tgtatcacgg ccttaatttt atcgaaaaca cggattttag
      241 tatgcacatg actgtactcc tgtggagtac aaggaaatca taatcagcga ccaaaccatt
      301 tatcaacaga aaaccaacat attgatgtta tttttgtgac aaatgttaca aattcgaaac
      361 cactgtattt ttactgcaac aaaatctggt tgatctacaa gttacactgg ggtaaaaagt
      421 tgtccggaat gtttctgcgt agtaaagcct ctagccggat tgcctttgca aaaacgcagg
      481 aacgttcccc ccttcatcag tactcctgac gctgtaagga gctgactgat gcagacaggg
      541 ctgatgatat ctaataaaag ctatctcatt tttgcgcgta cataaaataa atcaggcact
      601 tccctacact ccccctccgg aattgagggg gttttcttta caatgtaaat aaattgcttg
      661 cgtaaagttc aaaaaaactg caaattttta ctgtagtgtc gagcttcatg tagctcacca
      721 gggcatggac taaaaagaag taaacccatc ttttaccccc ccttatacgg ggggttcttt
      781 atagcacaaa gaacacttat aatttatcat ctcacagcta taaatataaa agaataatat
      841 tctaaactac agttatttat gcagaacata acatcaaact cacaataaat ataaaacaat
      901 tattttataa ctgttcactt attatattca gacatatcag aaacacgact caattctttt
      961 ttatttaaca aaatccataa aaaacttatg gttgaaatca catttaaact gtgaaggata
     1021 tcatccggag aaatttttca atccgacact ccatagaatg gacctttaag actttatcct
     1081 catcatgtat gaggattttt tttctggcaa acagtatctt tcagtaatcc ttgctcagtt
     1141 ccgattctga cgtatccccg tgtggatctg aaaaggagat aattacgctc tgtggccaac
     1201 aggaataact caagcagctc ctctctgata ccatcagaag aagcattaaa cgcattctgg
     1261 cgtcgattac agaagaagcg gaacgcgttc caggctcctg ttgctataca cactacccac
     1321 actaacccca tggtttttga gcgccagaaa cgcgtccgta tgcgttttaa cgcgttcctg
     1381 tgttttcagc aaggcctgac tgaagctcgt tcctagtgcc ctgaaccaac cattatccaa
     1441 aaaaatgcac cggaaacgcg ctgacaacac tttagcctgt ctttggttac catcccagcg
     1501 acgggccatc cagtgtccgt tcctgtattt ccagatggcg ctcacgctct aggaatagct
     1561 catgttcacg ttcctgtatc agtagttcgt cccttatctc ctgctctcgc tgtataactg
     1621 gctcaagtgt tctgtctgct cgctcaagtg ctgcacctgc tgactcaact gcatgacccg
     1681 ctcgttcagc atcgcgttgt cccgttgcgt aagcgaaaac atcttctgca attccacgaa
     1741 ggcgctctcc cattcgctca gccgctgcat atagtcctgt tgcagctgct ctaaggcgtt
     1801 cagcaaatgt gtttccagct ctgtcattct gtgtcactcc ttcagatgta cccactcttt
     1861 cccctgaaag ggaatcacct ctgctgattt cccgtacgga aggaccagaa atttcctgtt
     1921 cccgtcctgc acaaactcca cgccccatgt cttcgcgttc agtttctgca atgtctcttc
     1981 ctgcttcctg atttcttcca ggttcgtctg tatccgtccg ccaagatacc agagcgtccc
     2041 gccactcgcg gtaaacagga gaaagactat ccccagtaac atcatgcccg tattccctgc
     2101 cagctttaac acgtccttcc tgtgctgcat catcgcctct ttcacccctt ccctgtgttt
     2161 ttccagcgat ttctctgtcg aggctgtgaa cagggctata gcgtctctga ttttcgtctc
     2221 gtttgaggtc acagcctcgc tgacagactc gccgagcttc ctgaactcgt cgttcagcat
     2281 tttctctgta gattcggctc tctctttcag ctttttctcg aactccgcgc ccgtctgtaa
     2341 aagattgctc ataaaacgct cctttcagcc tgatattctt cccgccgttc gggtctgcaa
     2401 tgctgatgct gcttcgcgtc accctgacca cttcaagccc cgcctctgtg agcgcctgaa
     2461 tcacatccag acggcctttt atctccccta catgataaag agcgtctatc ccgcgtgtga
     2521 cgctctcagc aagcgcctgt ttcgtttccg gcaggttatc agagagagtc agtatccggc
     2581 ggttctccgg ggcgttgggg tcatgcagcc cgtaatggtg attcaccagc gtctgccagg
     2641 catcaattct cggcctgtct gcccggtcgt agtacggctg gagacgtttt ccggtctgta
     2701 gctccatgtt cggaatgaca aaattcagct caagccgtcc cttgtcctgg tgctccaccc
     2761 acaggatgct gtactgattc ttttcgagac ctggcatcag tacgcgctca aagctcgcca
     2821 tcactttttc acgtcctccc ggcggcagct ccttctccgc gaacgacaga acgcctgacg
     2881 tgtatttctt cgcaaatggc gtggcatcga tgagttcccg gacttcttcc ggattaccct
     2941 gaagcaccgt tgcgccttcg cggttacgct ccctccccag caggtaatca accggaccac
     3001 tgccaccacc ttttcccctg gcatgaaatt taactatcat cccgcgcccc ctgttccctg
     3061 acggccagac gcagccggcg cagctcatcc ccgatggcca tcagtgcggc caccacctga
     3121 acccgatcac aggaagacca ctgcccgctg ttcaccttcc tcgcggtctg attcaggtta
     3181 ttcccgatgg cggccagctg acgcagtaac ggcggtgcca gtgtcggcag cttcccggaa
     3241 cgggcaaccg gctcccccag gcagacccgc cgcatccata ccgccagttg tttaccctca
     3301 cagcgttcca gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctcg
     3361 cgtttcatcg gtatcattac ccccatgaac agaaatcccc cttacacgga ggcatcagtg
     3421 actaaacagg aaaaaaccgc ccttaacatg gcccgcttca tcagaggcca gacattaacg
     3481 ctgctggaga aactcaacga actggacgcg gatgaacagg cagatatctg tgaatcgctt
     3541 cacgaccacg ccgatgagct ttaccgcagc tgcctcgcac gtttcgggga tgacggtgaa
     3601 aacctctgac acatgcagct cccggagacg gacacagctt gtctgtgagc ggatgccggg
     3661 agcagacaag cccgtcaggg cgcgtcagca ggttttagcg ggtgtcgggg cgcagccctg
     3721 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagtgcgga
     3781 ttgtatgaaa agtgcaccat agccgatgtg aaatgccgca cagatgcgta aggagaaaat
     3841 gcaacttccg acactcttcc gcttcctcgc tcactgactc gctacgctcg gtcgttcggc
     3901 tgcggcgagc ggtaccggcg cactcaaagg cggtaatacg gttatccaca gaatcagggg
     3961 ataaggccgg aaagaacatg tgagcaaaaa ggcaaaggcc aacgaaaggc cgcgccgctg
     4021 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     4081 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     4141 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     4201 ggaagcgtgg cgctttctca tagctcacgc tgttggtatc tcggcttggt gtaggtcgtt
     4261 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     4321 ggtaactatc gtcttgagtc caacccggta aggcacgcct taacgccact ggcagcagcc
     4381 actggtaacc ggattagcag agcgatgatg gcacaaacgg tgctacagag ttcttgaagt
     4441 agtggcccga ctacggctac actgaaagga cagtatttgg tatctgcgct ctgctgaagc
     4501 cagttacctt ggttaagcag ttccccaact gacctaacct tcgatcaaac cgcctcccca
     4561 ggcggttttt tcgtttacag gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     4621 ccttttaact tttctactga accgcgatcc ccgtcagttt agaagaggag gatggtgcga
     4681 cagtccctcc ctgaacatcg cggatgtaat tagcttggca gcctgatagc aaagccatca
     4741 agacaagcat ccctcctgat aatatcattt tcatatattc cctcattaac acatacccct
     4801 aatcttagga tgaccattac ttgtacagca tgatatgctt atacacatct acaactaaat
     4861 taataataat gaaaagcttg gagagttata ccaatgagaa aagagcacac tgacaataaa
     4921 gatttaaaca ggacgctttt tttgattact tttgcaggca taacaccatt aatcattatt
     4981 tttataactt atacatcaaa ccccaagttc tatttaatta gcatcatttt tgataacaca
     5041 caaaatatac cttcaataat ttccgcatac aatcctgtga tgacaaaggt gatggatatc
     5101 tacgggaaaa gcgctccctt actggcactt attgctttta ctctccaact ccgcgacaga
     5161 aagctagaga caatagccaa cagagaaaaa ctaattactg ccagcatatt tagtccattt
     5221 ttttatgctt tttatgcata tttctttctg tggaataatt tcgaattaac cactgcaggt
     5281 aggactgtca gatggatgtc cgataatgat tttactttgt ttatttttta cgcctgcctc
     5341 tatttttgct ctttctttat gacttatgct ctgtgttata ttccggttgt gtcatataaa
     5401 ctctggaaag aaaggtagtc ttcttgacta cctttcgact ctaggttaat acgcagggcg
     5461 aataatttcg ttatttaacc tgtctataaa tttgtcatca atcaatgctc caataaaact
     5521 ggctataata ataccgataa tccctacgac tgcagctgat gcccccacag caagcaggta
     5581 agctccaagg gtggcagaaa aaatagcaag agccacagca gaagcaatac cactgacaac
     5641 ccaggactcg acctcccgca ttaacggccc ccagtttccg gtctgatatc cctctatact
     5701 cttctcccgg acgttatttg ccttcatcac ataatctgcg gctttaaatg tcttacccag
     5761 cgcggcaaac ttattcccca tatcctctgc atcaaatgct ttccaggcat tcacaatgac
     5821 atccctgtct gcagcattaa ttttaagatc aggattagcc atcagtttat tgacagaagc
     5881 catcgcctca tcataactac ggatcgtctt accttgaaag tttttgatat tacctgctat
     5941 ctcacgagaa agagctttat atttatcgcc aagatattct ccgaccttat cacccacact
     6001 aatgataatt tcactggctt ttgtcagaac agctttctcg ttatttgctt ttgcttcaga
     6061 ttcctttctg gctttttcag cttcagcttt tgcttttgct tcagcagccg ctttttcagc
     6121 ttcagctttc gcctttgcat cagcttttgc tttttctgct gccgccttgt tcttttctgc
     6181 ctgctgtttc cttgcagtca gtccggaaac ctgtgcctgc agcgcattaa tatcactgac
     6241 cagtaaatca tttttgttcc gggcatcatc atatgctctc tgagcatcac gaatacggtg
     6301 ataagaatgg ttcttaaata ctttttttag ttcttcaacc tctttctgtt tttgtttcat
     6361 aaggcttaat aatggtacct gttgtttctt cttctccgct attttaagat ttacgtcgtt
     6421 aatggctttc acggtctcat taataatccg gttcgccgca tcaacatcct tctgtgaggc
     6481 cgacattccg gatgatatct ggttgagaat gagcgccaaa ttaaccgcat ccaccacccg
     6541 ttcagtaact cgccccatat cttttttcag atttaccggg aaatcctgat atgccagact
     6601 ccaggctgca acaatctgtc tgacagcctc tccctgtttt gccagtggaa gggaatcgaa
     6661 agcctgccgc attgctttgt actctgcttt attcatcacc tcttcaggca agagaacacc
     6721 agtcacatag ccttttttga taatccggac acttttgatg gcgttattaa ccagccctac
     6781 attcactgga ccagcccctg tccagctgtc cttcgccaca gcatgccatt ctccggcggg
     6841 tttctgtccg ttcacaaaac tctccacaga agtttcgctt ccgggagtac ttgctcctga
     6901 tgcattaagg cggtgaccgg tgtctatttc gcccacagcc ccaataacac cagaagcgtt
     6961 atatcccatg accagccctc ccaaaccaag ctgcttggcc tgagtggagg ttatcccctc
     7021 aatggtgacc tgcataacac cagaaggagc aatacttccc agaatcattt ttgcttcagg
     7081 attcattttt ctcgcagcaa tcagcatatt caccactttc tgtttaagtt tctgatcatt
     7141 ctgaatggca gaaatctgct gactcacaga attgctactg tttgcgttat ctccgcgctg
     7201 gcctccacta tttccatgca taccaccgcc tggagctgga ccaccacttt cagaactcca
     7261 gccagtaccg tcaccatacc caccgtaatt aaaccccggc atattcattc ctctttgact
     7321 tttaaaacaa accattaaaa acaaatactg tatacataac cactggtttt atgtacagta
     7381 caaaagctac gccgctgcat tttccctgtc aagactgtgg acttctattt ttgtgaaaac
     7441 gatcaaaaaa cagtctctca caccacgcgc tattcccgcc ggataccaca aaaaccggcg
     7501 caaacattac cgttctcaga cctcattatg ttttactgaa accatgagat gagacaccta
     7561 tgggacactg taactttatg gcatggcaca cactccggga cgcactgaaa atgactggaa
     7621 gatcgcgttc acagttttac cgtgatatgc gctcaggtct tgtcagttac cgtaccagcc
     7681 aggacggacg aagggagttt gaaaccagtg aactgctccg ggcatacggc gaattaaagc
     7741 aaaatgagac acaagaaagg cacactgtgg gacaggcaga aaatccacat gatcagcaga
     7801 cagaacgcat tctccgggaa ctgaatgagc tgaaacaatg cctgacgctg atgcttgagg
     7861 ataaacaggc acaggatatg gatcgcaggc accaggaatc agaacgggaa cagttacaga
     7921 atgagatagc ccagctcagg caggcactgg aacaggaaaa gaaacgggga ttctggtcca
     7981 gactcttcgg tcactgaact cggtcagagg ctgatgataa gatagtattc ggataataac
     8041 tcaccgagaa taaatacttt aaggtaagga gacacgtatg aaacgttctg gtggcacacg
     8101 ctatctgtat cttatatccc tgataactgt ggctgcagcc ctcacggcct gtaccccgaa
     8161 aggcagtgtg gaacaacata cccgacatta tgtgtacgct tccgatgacc gttctgaccc
     8221 taacttctac accaacaagg ccgacaccac acggatgatg attccgttct ttcagcagtt
     8281 cagggagatg ggggagaaag acaaagcagc cggagtatca gcagaaacgg cacagcaacg
     8341 cataaaagaa ttccactcag aaaaatttct tcagtctctc agaagcacaa ccacctttgc
     8401 gggcagaaaa tatacgaaca gtgatatgcc atcaccggaa aaaatgaagc tactggctga
     8461 cacgatttct gcggtttatc tcgatggcta cgagggaaga cagtaaatgg atttaccata
     8521 atcccttaat tgtacgcacc gctgaaatgc attcagcgca atcacggcag cagacaggta
     8581 aaaatcgcaa caaccccccc gaaaaactac cgcgatcgcg cctgataaat tttaacctta
     8641 tgcataccta tgcatccagg tgaatcacaa gcgaatttcc ttactgagac gactgaaacg
     8701 gttttttttc acaattttca gctgttttcg attccacaaa atagaagtta caggataggg
     8761 attaatcccc ttcgccgcca aggggagcag gatgagtcag gggaatgtct attcacctgc
     8821 gctgtacagg ctgccaggta aaagtattcg ctgcggcacg atactctttt gccctcatag
     8881 cctttgcgct tcttactttt cgttcttcca gaatcattct taagacattt tcccgcactt
     8941 ttctctaact tcccgtggca atttatctct gccggactga accgctccgg aaatcctgga
     9001 gactaaactt cctgagaaag atgtaaacag gatgactaaa aatactcgtt tttcccccga
     9061 agtccgtcaa cgggcagttc gtatggttct ggaaagccag ggcgaatatg actcacaatg
     9121 ggcggcaatt tgttccattg ctccaaagat tggctgtacg ccagagaccc tgcgtgtctg
     9181 ggttcgccag catgagcggg ataccgggta ttacctttcg aagaaatttt tgacatcctc
     9241 tacgccctga aggaccgggt tttacggcgt accagataaa aaaacccctc tactgaagag
     9301 gggtagtgta aggaacttgc tgattttctt gatattatta accatcaata actgagcata
     9361 caaaaaataa caaaactctg acacagatac aaccatccct ctttgtataa gaccgatgtg
     9421 catctcaaaa aagaagcgtg tttctggaac acagaacaac aatgcacatt gtagccaaca
     9481 aattcacacc agaa
//
