LOCUS       NZ_CP120553             6988 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain B1172 plasmid pB1172_6988, complete
            sequence.
ACCESSION   NZ_CP120553
VERSION     NZ_CP120553.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33748627
            Assembly: GCF_029536615.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6988)
  AUTHORS   Fedrova,P., Hrala,M., Micenkova,L., Tom,N., Bosak,J. and Smajs,D.
  TITLE     Complete genome sequences of five Escherichia coli strains with
            probiotic features
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6988)
  AUTHORS   Fedrova,P., Hrala,M., Micenkova,L., Tom,N., Bosak,J. and Smajs,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Department of Biology, Masaryk University,
            Kamenice 5, Brno 62500, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 416x
            Sequencing Technology :: Illumina; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029536615.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 04:44:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,290
            CDSs (total)                      :: 5,174
            Genes (coding)                    :: 4,916
            CDSs (with protein)               :: 4,916
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 110 of 258
            Pseudo Genes (incomplete)         :: 182 of 258
            Pseudo Genes (internal stop)      :: 45 of 258
            Pseudo Genes (multiple problems)  :: 64 of 258
            Pseudo Genes (short protein)      :: 1 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP120553.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="B1172"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pB1172_6988"
                     /geo_loc_name="Czech Republic"
                     /collection_date="2009"
     gene            complement(589..780)
                     /locus_tag="P5710_RS26295"
                     /old_locus_tag="P5710_26295"
     CDS             complement(589..780)
                     /locus_tag="P5710_RS26295"
                     /old_locus_tag="P5710_26295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            848..1171
                     /locus_tag="P5710_RS26300"
                     /old_locus_tag="P5710_26300"
     CDS             848..1171
                     /locus_tag="P5710_RS26300"
                     /old_locus_tag="P5710_26300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1380..>1814
                     /locus_tag="P5710_RS26305"
                     /old_locus_tag="P5710_26305"
                     /pseudo=""
     CDS             1380..>1814
                     /locus_tag="P5710_RS26305"
                     /old_locus_tag="P5710_26305"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1849..2409
                     /locus_tag="P5710_RS26310"
                     /old_locus_tag="P5710_26310"
     CDS             1849..2409
                     /locus_tag="P5710_RS26310"
                     /old_locus_tag="P5710_26310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067492.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            2416..2649
                     /locus_tag="P5710_RS26315"
                     /old_locus_tag="P5710_26315"
     CDS             2416..2649
                     /locus_tag="P5710_RS26315"
                     /old_locus_tag="P5710_26315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137427.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDHLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3133..3555)
                     /locus_tag="P5710_RS26320"
                     /old_locus_tag="P5710_26320"
     CDS             complement(3133..3555)
                     /locus_tag="P5710_RS26320"
                     /old_locus_tag="P5710_26320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001255518.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTA
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            3677..3955
                     /locus_tag="P5710_RS26325"
                     /old_locus_tag="P5710_26325"
     CDS             3677..3955
                     /locus_tag="P5710_RS26325"
                     /old_locus_tag="P5710_26325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000710675.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(3947..4237)
                     /locus_tag="P5710_RS26330"
                     /old_locus_tag="P5710_26330"
     CDS             complement(3947..4237)
                     /locus_tag="P5710_RS26330"
                     /old_locus_tag="P5710_26330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000047032.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            4477..6123
                     /gene="cka"
                     /locus_tag="P5710_RS26335"
                     /old_locus_tag="P5710_26335"
     CDS             4477..6123
                     /gene="cka"
                     /locus_tag="P5710_RS26335"
                     /old_locus_tag="P5710_26335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="WP_011264160.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGHG
                     NNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNPFG
                     GVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVGNVINEKQK
                     QKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKLNSYK
                     NTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVAEAEKRLAEAKAELAKAESD
                     VQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAVENELKN
                     AVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEKYAKIAQD
                     LAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVNYDELSKNLTKIS
                     KSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGVP
                     LGFWGIAIVTGIVSSYIGDDELSKLNELLGI"
                     /name="cka"
     gene            complement(6120..6410)
                     /gene="cki"
                     /locus_tag="P5710_RS26340"
                     /old_locus_tag="P5710_26340"
     CDS             complement(6120..6410)
                     /gene="cki"
                     /locus_tag="P5710_RS26340"
                     /old_locus_tag="P5710_26340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_011264161.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            6440..6571
                     /locus_tag="P5710_RS26345"
                     /old_locus_tag="P5710_26345"
     CDS             6440..6571
                     /locus_tag="P5710_RS26345"
                     /old_locus_tag="P5710_26345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_077521118.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            complement(6766..6909)
                     /locus_tag="P5710_RS26350"
                     /old_locus_tag="P5710_26350"
     CDS             complement(6766..6909)
                     /locus_tag="P5710_RS26350"
                     /old_locus_tag="P5710_26350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_152896351.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWIVDLDTQ
                     NS"
                     /name="replication initiation protein"
     Typing          417..504
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          complement(3..406)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.103"
                     /coverage="99.0"
                     /evalue="6.380000000000001e-167"
     Typing          complement(6801..6988)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.413"
                     /coverage="99.0"
                     /evalue="3.96e-88"
     Typing          1162..2756
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.624"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
       61 agggagccac cagggggaaa cgcctggtat ctttaagtcc tgtcgggttt cgccacctct
      121 gacttgagcg tcggtttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
      181 gctgcgtggc cttttccggg ttcttgtttt ttgctcacat gtttcttttg gtgttatccc
      241 ctgattctgt ggataaccgc attaccgtct ttgagtgagc tgataccgct cgccgcagcc
      301 gaacgaccga gcggagcgag tcagtgagcg aggaagcgga aaagcgcctg gacgagcatt
      361 ttctccttac gcatctgtgc ggcatttcac acccggcatg gtgcactttt catacaatcc
      421 tcattgatgc caaatgatta agccagtata cactccgcta tcgctacgtg actgggtcat
      481 ggctgcgccc cgacacccgc taaaacccgc tgacgcgccc tgacgggctt gtctgctccc
      541 ggcatccgct cacaggccag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
      601 accgtcatcc ccgaagcgtg cgaggcagct gcggtaaagc tcgtcagcgt ggtcgtgaag
      661 cgcttcgcag atgtcagcct ggtcgtcggc gtccagttca ttcagttttt ccagcagggt
      721 cagcgtctgg cttctgatga agcgggccat gttaagggcg gttttttcct gtttagtcac
      781 agttctgttt ctccatgtgt ggggagtctg tcggaggttt ttaatgatac cggtgaaacg
      841 cgaaaggatg ctcacgatac gggtcacgga tgaggaacat gcgcggctgc tggcacgttg
      901 tgagggaaaa caacttgcgt catggatgcg taaggtctgc ctgggggcac cgccatcgaa
      961 aacgtcaggt cttccgacac tggcaccgcc gctgttgcgc cagttcgctt cggtcggcaa
     1021 taacctgaat cagatagccc ggaagataaa cagcggacag tggtcggggc atgaccgggt
     1081 tcatgtggtg gctgcactga tggccatcgg acgtgaactg agtgagctga gggatgaggt
     1141 ccgtaaacag ggagaacggg atgatagtta agtttcatgc caggggaaaa ggcggtggca
     1201 gtggtccggt tgattacctg ctgggccggg agcgtaaccg ggaaggggcg agagttctgc
     1261 ggggtgctcc cgaagaggtg cgggaactca tcgatgccac gccgtttgcg aaaaaataca
     1321 cctcgggggt tctgtctttt gcagagcaga ccctgccacc gggagagcgt gaaagggtga
     1381 tggagagctt tgagcgggta ctgatgcccg gtcttgaaaa aaatcagtac agcatcctgt
     1441 gggtggagca ccaggacaag gggcgtcttg agctgaattt tgtcatcccg aatatggagc
     1501 tggcgagcgg aaaacggctc cagccctatt acgaccgagc agaccgtccg cgaatcaatg
     1561 cctggcagac actggtgaat caccattacg ggctgcacga cccgaacgca ccggagaaca
     1621 ggcggacact tgtcaccccg aataatctgc cgaaagcgaa acaggaggcc gcagaagcga
     1681 ttacgagggg actggaggct ctttaccatg ccggagcgct aaaaacgcgt caggatgtca
     1741 ctgaggcgct tacagcggca ggttttgagg ttgtgaggac aaccaggagc agtatcagta
     1801 tcgccgaccc ggagggtggg cgtaatctca ggctgaaagg ggcgctgtat gagcaatctt
     1861 ttacaaatgg ggacagactt cgagaagaaa ctgaaagagc gtgccgtctc tacagagaac
     1921 atgctgaaca gcgagttcag gaagctcgaa gcgtctgtag acagggaact gaacttaaac
     1981 gggcagaaaa tcagagacgc tatcagcgca cacacagcag cggtgaaaga acaactggag
     2041 acgctgaaca gtgccgtgaa cgcgcagttc agcgccacag agaacgaact ggcccggcgg
     2101 caggaagagc tgctctggaa gctggtcaaa gggcggatac tgtacccgtc gctgacggcg
     2161 ctttgcgtca cgggcgggat ttttctggcg tcatgggggc tgattcagtg gcaggaaagc
     2221 cggattgcgg cgaacatcct ggctatcagg gaccaggaag agacgctggc gaaactccgg
     2281 gaaaaaacat ggggtgtgac gtatcacgag ggcaggaacg ggaaatttct ggtgttgccg
     2341 tccggggtga agggggaaaa caactggacg gtggagaaga agaacgcagt aagactggtc
     2401 agggagtaag tgagtatgac agagctggaa atgcacttac tgaacgcctt agagcaattg
     2461 cagcaggact atacgcagcg gctgaaagac tgggagagcg ccttcacgga tttgcagcat
     2521 atgttcgagc tgacacgaca ggagaacggg caactgaggg aacagtgcga cgacttgagt
     2581 cagcaggtgc agagattagc cgggcaggtg gaccacttga gtcggttatt tatcacgaac
     2641 agcagataag ggatgaacag gaaaggcagg cacgcctgga acgggagcgt gcgcggtcct
     2701 ggcatgaaga gcaggaacgg gaagcacggg tatggcgtgg cccgtcaata gggatgtgac
     2761 ggtgagttgt tccgggaggt tgccagatgg taccggaacg cgttaaaaac gcatccgggc
     2821 gcgattctgg cgtgtcgttt ttgttgctgt gttttgcctc tttgccagat cgcgctcatt
     2881 cctcagcctc atcccgcgat gtctgcgtat gtcatcaact gaaaagatgg aaaaaaaaca
     2941 gtgtcatgag gtttacgcag gcaaaactag cgttattcac atggctgaat agctatgcat
     3001 acggttaaaa tttatcaggt gcgatcgcgg cagcttttcg ggtggtttgt tgccattttt
     3061 acctgtcagc agccgtgatc gcgctgaacg catttcagcg gtgcgtacaa ttaagggatt
     3121 atggtaaatc ccttactgtc tgccctcgta tccatcgaga taaaccgcag aaatggtgtc
     3181 tgccattagt ttcatttttt tcggcgaagg catatcgctg tttgtgtatt ttctgccagc
     3241 aaaggctgtt gtgctccgga gtgagtgaaa aaatttttct gagtggaatt cttttacacg
     3301 ttgctgtgcc gtttctgctg atactccggc tgctttgtct ttcatcccca tatcccggaa
     3361 ctgctgaaag aacgggagca tcatacgtat ggtgtctgct ttctgggtgt agaagttagg
     3421 atcaaatcca tcatctgaag cataaacata atgccgggta tgtcgttcca ggctggtctt
     3481 tggtgtgcat cctgtaagag ctattgttgc tgttatcaga ggtatcagaa gcagcctggc
     3541 agcggcagat ctcataaaca tctccttgcg tttttaatct tgatactcag ggagttatat
     3601 ctccgaagag tatctcttat catacgtcgg tttactccca atgtgatttg atggtcaatg
     3661 agtcaggaat aatcccgtga aaaaatgtgt gccagtcctg ctttttagtg ttctttctgc
     3721 ttcggttgcc agtcatgcgg acagcaatgt ttttcaacaa aaggccgaga agacaaaaaa
     3781 cggcattgtt gcggatgtta atgccagaca gaaaatgttc agtaacagac tgaagataca
     3841 acttcagatc gatgagttga gagcagagct ggctggcacc agagatgccc aaaaaagaga
     3901 ggatttgcgt aaaaaactgg agtctctgga gcggcaaaag aaatccttac gctgatgggg
     3961 gcgtattttc cccggtttct tccataacct ttttaccgtt cagatattta cgggcgaggc
     4021 gaatgcgtct tgcctggtat accgccgagt aagcctgggt gggattcaca ccattactga
     4081 tttcactgac cacatgacgc tgatcagcca gtgtcagcgt gtaaacctgt ttctggatga
     4141 ggtcacgggc tttgtcatgc tcatatccgg cagcaacaag ggtgtcgaga agtcgtttgt
     4201 ctgttttgtt cagtttatgg cggggtgatt tgctcacatc gttctccgtt ttttcatgat
     4261 ccaacagttt tatatctcat cctgatttgc tccggtgttc tgtttcgacg ttctcaatgc
     4321 ctgcctcccc atagtgtgat ccttattgca aaaatgaaaa tccatgctct tgacatggac
     4381 aatgctgagt agtaggtttt actgtatata aaaccagtgg ttatatatac agtatattgt
     4441 ttttaattta ttgttttaat tatcaaagag gaatttatgg ctaaagaact aagtggatat
     4501 ggaccaactg ctggtgagtc tatgggggga acaggggcga atctgaatca gcaaggtgga
     4561 aataataata gtaatagtgg ggttcactgg ggtggtggct ctgggcatgg taataatggt
     4621 ggtcagggaa attcaaattc atctggttct acatcaactg tgatgaaaac cggtgagtca
     4681 tatctgactc cgtggggtga tgttgtcatt aataatgatg gccttccggt gatgaatggt
     4741 attgttatga cagaagagaa ttcaactctg gttgataatc catttggagg ggtctccaga
     4801 gttcttaatt ctttaattag tgatatgcca tcactttttg cagaaagctc aggaaataac
     4861 aacaataaca ctgcttcagt taatactgca ccaacgaatg ctcaggtcag tgatatggat
     4921 aagagttcga aggtggtggg taatgtaatt aatgaaaaac aaaagcagaa gaataaaatt
     4981 gctacacaga ttagtgaaaa acagaagaaa atcgaggaga tgaaaaaagt attcaagcat
     5041 cattcttatc atggtatcac tgatcttgag agggatgttg atgagttgca aaaaaaatca
     5101 aatcagcttg atgcagatat tagcaaactt aattcttata aaaatacatt gcaaagtaaa
     5161 attggtgatg tgaataaaca gaaggaagct gaagaaaagg ctcgtgaaaa tgcagaggtc
     5221 gctgaacatg aaactcttaa tgaagagaaa caggcggtgg cggaagcaga gaagcgttta
     5281 gctgaggcga aagctgaact ggcgaaggct gaaagtgatg tacagagtaa gcaagctact
     5341 gtttccagag ttgcaggaga gcttgaaaac gctcagaaga gtgttgacgt gaaggtcacc
     5401 ggatttccgg gatggcgtga cgttcagaaa aaactacagc gtcagcttga agctaagcag
     5461 gctgagtact ccgcagttga aaatgaactt aagaatgctg ttagtttcag ggatggtaag
     5521 gccgctgagg ttaaagaggc agagcagaaa ttaaaagaag ctcaggatgc tcttgaaaag
     5581 agccagataa aagatgctgt tgatactatg gttggatttt atcaatatat aaccgaacaa
     5641 tatggagaaa aatacgcaaa aattgctcag gatttagcgg aaaagtctaa aggaaagaaa
     5701 attcaaggtg ttgatgaagc tctggcagca tttgaaaagt ataagaatgt actggataag
     5761 aaattcagta aggttgatcg ggatgctatt tttaatgcgt tagagtctgt taattatgat
     5821 gagttatcta agaatttaac gaagatatca aagtcgctta aaatcaccag tcgagtatct
     5881 tttctatatg atgttggttc tgattttaaa aatgcaatag aaactggtaa ctggcgacct
     5941 ttgtttgtca cgctggagaa aagtgcggtc gatgtaggtg tggcaaaaat tgtggcatta
     6001 atgtttagtt ttattgttgg tgtgcctctt ggtttctggg ggattgcaat tgtcacaggt
     6061 attgtttctt cttacatagg ggatgatgag ttgagcaaac ttaatgagtt gttagggatt
     6121 taatttctct ttagagtgta atatatgaaa cttggcaatc ctactgggat tgccattaat
     6181 aaataaaaca atgcagataa cttgcataag taccagggtt ctttttttaa ctttgtattt
     6241 tctttgatat atctagatat ggttaattta gagtatggat atagcaatac atgtattgat
     6301 gatataaata taaataacaa aggagtgtta tcattgtcgt taaatattaa aataagaatc
     6361 catggtataa gtgattcagg taaattatgt aggtagtatt ttaaatgcat aagtattttt
     6421 acttttgttg gaaagaaata tgaaaataat aatttttcta attacaatat tattcttgtc
     6481 aggatgtcaa gcaaactata ttcgtgatgt acaaggagga accgttgcac catcttcgtc
     6541 ttctgaactg acggggatcg cggttcagta gaaaagatca aaggatcttc ttgagatcct
     6601 ttttttctgc gcgtaatctg ctgcctgtaa acgaaaaaac cgcctgggga ggcggtttag
     6661 tcgaaggtta agtcagttgg ggactgctta accgggtaac tggctttagt ggagcacaga
     6721 taccaaatgc tgtccttcca gtgtagccgc tataaggctt caacttcaag agttctgcgt
     6781 atctaaatcc actatccagt taccaatggc tgctgccagt ggcgttaagt cgtgtcttac
     6841 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     6901 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat accaacagcg
     6961 tgagctatga gaaagcgcca cgcttccc
//
