LOCUS       NZ_CP120552            11599 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain B1172 plasmid pB1172_11599, complete
            sequence.
ACCESSION   NZ_CP120552
VERSION     NZ_CP120552.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33748627
            Assembly: GCF_029536615.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11599)
  AUTHORS   Fedrova,P., Hrala,M., Micenkova,L., Tom,N., Bosak,J. and Smajs,D.
  TITLE     Complete genome sequences of five Escherichia coli strains with
            probiotic features
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11599)
  AUTHORS   Fedrova,P., Hrala,M., Micenkova,L., Tom,N., Bosak,J. and Smajs,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Department of Biology, Masaryk University,
            Kamenice 5, Brno 62500, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 416x
            Sequencing Technology :: Illumina; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029536615.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 04:44:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,290
            CDSs (total)                      :: 5,174
            Genes (coding)                    :: 4,916
            CDSs (with protein)               :: 4,916
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 110 of 258
            Pseudo Genes (incomplete)         :: 182 of 258
            Pseudo Genes (internal stop)      :: 45 of 258
            Pseudo Genes (multiple problems)  :: 64 of 258
            Pseudo Genes (short protein)      :: 1 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP120552.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="B1172"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pB1172_11599"
                     /geo_loc_name="Czech Republic"
                     /collection_date="2009"
     gene            join(11585..11599,1..144)
                     /locus_tag="P5710_RS26225"
                     /old_locus_tag="P5710_26225"
     CDS             join(11585..11599,1..144)
                     /locus_tag="P5710_RS26225"
                     /old_locus_tag="P5710_26225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            642..869
                     /locus_tag="P5710_RS26230"
                     /old_locus_tag="P5710_26230"
     CDS             642..869
                     /locus_tag="P5710_RS26230"
                     /old_locus_tag="P5710_26230"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_229471606.1"
                     /translation="MLYAIVISTILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            complement(866..2434)
                     /gene="cea"
                     /locus_tag="P5710_RS26235"
                     /old_locus_tag="P5710_26235"
     CDS             complement(866..2434)
                     /gene="cea"
                     /locus_tag="P5710_RS26235"
                     /old_locus_tag="P5710_26235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            2694..3131
                     /locus_tag="P5710_RS26240"
                     /old_locus_tag="P5710_26240"
     CDS             2694..3131
                     /locus_tag="P5710_RS26240"
                     /old_locus_tag="P5710_26240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021532742.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSKGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            3212..3631
                     /locus_tag="P5710_RS26245"
                     /old_locus_tag="P5710_26245"
     CDS             3212..3631
                     /locus_tag="P5710_RS26245"
                     /old_locus_tag="P5710_26245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(3948..4181)
                     /locus_tag="P5710_RS26250"
                     /old_locus_tag="P5710_26250"
     CDS             complement(3948..4181)
                     /locus_tag="P5710_RS26250"
                     /old_locus_tag="P5710_26250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4188..4706)
                     /locus_tag="P5710_RS26255"
                     /old_locus_tag="P5710_26255"
     CDS             complement(4188..4706)
                     /locus_tag="P5710_RS26255"
                     /old_locus_tag="P5710_26255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4744..5175)
                     /locus_tag="P5710_RS26260"
                     /old_locus_tag="P5710_26260"
                     /pseudo=""
     CDS             complement(<4744..5175)
                     /locus_tag="P5710_RS26260"
                     /old_locus_tag="P5710_26260"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5269..8274)
                     /locus_tag="P5710_RS26265"
                     /old_locus_tag="P5710_26265"
     CDS             complement(5269..8274)
                     /locus_tag="P5710_RS26265"
                     /old_locus_tag="P5710_26265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="WP_015586051.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHADNLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLGENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQASVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGALGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEVENVA"
                     /name="Tn3-like element Tn3 family transposase"
     gene            8437..8994
                     /locus_tag="P5710_RS26270"
                     /old_locus_tag="P5710_26270"
     CDS             8437..8994
                     /locus_tag="P5710_RS26270"
                     /old_locus_tag="P5710_26270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001217881.1"
                     /translation="MRIFGYARVSTSQQSLDIQIRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            9177..10037
                     /locus_tag="P5710_RS26275"
                     /old_locus_tag="P5710_26275"
     CDS             9177..10037
                     /locus_tag="P5710_RS26275"
                     /old_locus_tag="P5710_26275"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="WP_000027057.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="broad-spectrum class A beta-lactamase TEM-1"
     gene            complement(<10181..10348)
                     /locus_tag="P5710_RS26280"
                     /old_locus_tag="P5710_26280"
                     /pseudo=""
     CDS             complement(<10181..10348)
                     /locus_tag="P5710_RS26280"
                     /old_locus_tag="P5710_26280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /name="nuclease"
     gene            complement(10338..10661)
                     /locus_tag="P5710_RS26285"
                     /old_locus_tag="P5710_26285"
     CDS             complement(10338..10661)
                     /locus_tag="P5710_RS26285"
                     /old_locus_tag="P5710_26285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            10725..10916
                     /locus_tag="P5710_RS26290"
                     /old_locus_tag="P5710_26290"
     CDS             10725..10916
                     /locus_tag="P5710_RS26290"
                     /old_locus_tag="P5710_26290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     ARG             9178..10034
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(11001..11088)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          11099..11599
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.03"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3845..5247)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.474"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(10342..10691)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.436"
                     /coverage="99.0"
                     /evalue="1.5899999999999998e-68"
ORIGIN
        1 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaagg
       61 cacgccttaa cgccactggc agcagccact ggtaaccgga ttagcagagc gatgatggca
      121 caaacggtgc tacagagttc ttgaagtagt ggcccgacta cggctacact agaaggacag
      181 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      241 gatccggcaa acaaaccacc gttggtagcg gtggtttttt tgtttgcaag cagcagatta
      301 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctactgaa ccgcgatccc
      361 cgtcagttta gaagaggagg atggtgcgat ggtccctccc tgaacatcac gtatatagtt
      421 agcctgacat ccaacaagga ggtttatcgc gaatattccc acaaaaaatc ttttcctcat
      481 aactcgatcc ttataaaatg aaaagaatat atggcgaggt ttaatttatg agcttaagat
      541 actacataaa aaatatttta tttggcctgt actgcacact tatatatata taccttataa
      601 caaaaaacag cgaagggtat tatttccttg tgtcagataa gatgctatat gcaatagtga
      661 taagcactat tctatgtcca tattcaaaat atgctattga atacatagct tttaacttca
      721 taaagaaaga ttttttcgaa agaagaaaaa acctaaataa cgcccccgta gcaaaattaa
      781 acctatttat gctatataat ctactttgtt tggtcctagc aatcccattt ggattgctag
      841 gactttttat atcaataaag aataattaaa tccctaacac ctcatttata gtattaagtt
      901 tattcttatc aatataggag catagaattc ctgtaacaat agcaataccc caaataccta
      961 atgtagttcc agcaagcaag ctaaaaagta aagcaacaac ataactcacc cctgcatctg
     1021 cagctttctt ctctaatgta agaaatagtg gcttccagtc acctgtatcc ttaattttta
     1081 ggatatcaga taccacatca tatccaaaag aaacatgccc cgtaatcttc aagtacttgg
     1141 caaactgatc taaatgttta gcccagtcat catacttcac cgatgccaac gcattaaaaa
     1201 tagcatcacg atcggctttg ctgaatttct tatttaaaac atccttgtat ttttcaaaag
     1261 cagcgagagc ttcattcaca ttgccgattt tcttaccttt agacttatca gcaagttcct
     1321 gtgccatttt cgaatatttt tcaccatatt tttcagtcag cgtttgataa aagctaactg
     1381 ttgcatcaac agcatcctta atctgtgaat taaggagatt attctgtgct tttttcaaat
     1441 tttcttcagc ttcatgaaca cgagcgatac cggcattacg attattactg acctgagaaa
     1501 tagccttctg gatctgagtt atatcagcat ttatccggtt aatacgtgtt tctgatgctg
     1561 ttacctgttt ttgtttttct tctctaatct taccggcccc aacccgtcgt ctggttgctt
     1621 caaaaaaagg acggttctga agcggatcat tggctcttgg tgatagtttt ttgaccagct
     1681 catccagttc tttatattta gcggatgcct gagccagttc atttcgtttt ccagcgagcg
     1741 ttttcatttc tgcatcacgg gcatggatac tggagcttaa acgagaattg agagtcttaa
     1801 tctctccatc cattttcacc acttcagatt gtgcagcaga aagttttttt tgggcgatct
     1861 caacagcttt agcttcttca ctcaatgcag ccagtcgttt ctcttcagct tcagccagtt
     1921 tcaactggcg ttctgtttca gccttctccc gttcaatctc tttacgtcgt tgttctgctt
     1981 cctgaaaagc cttttctgct gcttccgctt ctttacgggc tttttcttct gctttcgcaa
     2041 ggcgcaaacg ctctgcttcc gcctgcatag ctgcattatt agcatgagca agctctgttg
     2101 ctgaaggcgt acgtgaggca ttgtgacgaa gagcctcatt cacgatatcc ttcaggcgct
     2161 gagtcagcgc atccctgttt gcctttgctt tcgcctgtgc ttccgctgca gcttttgccc
     2221 gggcagcctg ctctgcctgt gttttcttta attgagcagt agaccattta gcagttgcat
     2281 gaatagctgc agaactttca cttttactgc ctccttttcc acctccgccg ccagagccac
     2341 tcccgtcagg agtaccattc aaaagagtaa taattacctg tcccttatca tcataaggaa
     2401 caccatcttt atagtacgct accgcggttt ccattataaa atcctctttg acttttaaaa
     2461 caataagtta aaaataaata ctgtacatat aaccactggt tttatataca gcataaaagc
     2521 tacgccgctg cattttccct gtcaagactg tggacttcca tttttgtgaa aacgatcaaa
     2581 aaaacagtct ttcacaccac gcgctattct cgcccgatgc cacaaaaacc agcacaaaca
     2641 ttaccgttct cagacctcat tatgttttac tgaaactatg agatgagaca tctatgggac
     2701 actgtcactt tatggcatgg cacacactcc gggacgcact aaaaatgaca ggcagatcgc
     2761 gttcacagtt ttaccgtgat atgcgcgcag gccttgtcag ttaccgtacc ggcagggacg
     2821 gacgacggga gtttgaaacc agtgaactga tccgggcata cggcgaatta aagcagaatg
     2881 agacaccaga aaggcacagt aagggacatg cagaaaatcc acatgatcag cagacagaac
     2941 gcattctccg ggagctgaat gagctgaaac aatgcctgac gctgatgctt gaggataaac
     3001 aggcacagga tatggatcgc agacgccagg aagcagaacg ggaacagcta caaaatgaga
     3061 tagcccagct caggcaggca ctggaactgg aaaagaaacg gggattctgg tccaggttgt
     3121 tcggtcgctg aacgctgtca gagactgatg ataaaatagt cttcggataa taactcaccg
     3181 agaataaata ctttaaggta gggagacact catgagacgt accggaaaca aactttgtct
     3241 tatcgccatg ataacagcaa cagtagctct cacagcctgt accccaaagg gcagcgtgga
     3301 acaacatacc cggcattacg tatatgcttc tgatgacggt tttgatccca acttttccac
     3361 ccaaaaagcc gacacaacac gaatgatggt gccttttttt cggcagttct gggatatggg
     3421 agctaaagat aaagcgacag gaaaatcacg gagtgatgtg caacaacgca ttcagcagtt
     3481 tcacagccag gaatttttaa actcactccg gggcacaact caatttgcgg gtactgatta
     3541 ccgcagcaaa gaccttaccc cgaaaaaatc caggctgctg gctgacacga tttctgcggt
     3601 ttatctcgat ggctacgagg gcagacagta agtggattta ccataatccc ttattgtacg
     3661 caccgctaaa acgcgttcag cgcgatcacg gcagcagaca ggtaaaaatg gcaacaaacc
     3721 acccgaaaaa ctgccgcgat cgcgcctgat aaattttaac cgtatgaata cctatgcaac
     3781 cagagggtac aggccacatt acccccactt aatccactga agctgccatt tttcatggtt
     3841 tcaccatccc agcgaagggc catccagcgt gcgttcctgt atttccggct gacgctcccg
     3901 ttccagggat aacacatgtt cgcgctcctg tatcagccgt tcctctctta tctccagttc
     3961 tcgctgtata actggctcaa gcgttctgtc tgctcgctca agtgctgcac ctgctgactc
     4021 aactgcatga cccgctcgtt cagcatcgcg ttgtcccgtt gcgtaagcga aaacatcttc
     4081 tgcaattcca cgaaggcgct ctcccattcg ctcagccgct gcatatagtc ctgttgcagc
     4141 tgctctaagg cgttcagcaa atgtgtttcc agctctgtca ctctgtgtca ctccttcaga
     4201 tgtacccact ctttcccctg aaagggaatc acctccgctg atttcccgta cggaaggaca
     4261 aggaatttcc tgttcccgtc ctgcacaaac tccacgcccc atgtcttcgc gttcagtttc
     4321 tgcaatgtct cttcctgctt cctgatttct tccaggttcg cctgtatcct ccctccaaga
     4381 taccagagcg tcccgccact cgcggtaaac aggagaaaga ctatccccag taacatcatg
     4441 cccgtattcc ctgccagctt taacacgtcc ctcctgtgct gcatcatcgc ctctttcacc
     4501 ccttcccggt gtttttccag cgattcctct gtcgaggctg tgaacagggc tatagcgtct
     4561 ctgattttcg tctcgtttga tgtcacagcc tcgcttacag attcgccgag cctcctgaac
     4621 tcgttgttca gcattttctc tgtagattcg gctctctctt tcagcttttt ctcgaactcc
     4681 gcgcccgtct gcaaaagatt gctcataaaa tgctcctttc agcctgatat tcttcccgcc
     4741 gttcggatct gcaatgctga tactgcttcg cgtcaccctg atcacttcca gccccgcctc
     4801 agtgagcgcc tgaatcacat cctgacggcc ttttatctct ccggcatggt aaagtgcatc
     4861 tatacctcgc gtgacgccct cagcaagcgc ctgtttcgtt tcaggcaggt tatcagggag
     4921 tgtcagcgtc ctgcggttct ccggggcgtt cgggtcatgc agcccgtaat ggtgattcac
     4981 cagcgtctgc caggcatcaa ttctaggcct gtctgcgcgg tcgtagtacg gctggaggcg
     5041 ttttccggtc tgtagctcca tgttcggaat gacaaaattc agctcaagcc gtcccttgtc
     5101 ctggtgctcc acccacagga tgctgtactg atttttttcg agaccgggca tcagtacacg
     5161 ctcaaagctc gccatcactt tttcacgtcc tcccggcggc agctccttct ccgcgaacga
     5221 cagaacaccg gacgtggggt ctgacgctca gtggaacgaa aactcacgtt aagcaacgtt
     5281 ttctacctct gacgcctctt ttaatggtct cagatgtcct ttggtcacca gttctgccag
     5341 cgtgaaggaa taatggccga gcatattgat atgtccgtgg caaagcgggg agaggcgtgc
     5401 gatatcttca tcattcagtg tttcaccctg cgcccggaga tgatccaggg ctgcctgcat
     5461 ataaatagtg ttccataaca cgacggcgtt agtgaccagc cccagtgcgc ccagttgatc
     5521 ttcctgaccg tcggtatatc gttttcttat ctcacctttt tgaccgtgac agatggctct
     5581 ggcaacggca tggcggcttt ctccccgatt aagctgggtc agaatgcgcc ggcggtaatc
     5641 ttcatcatca atataattaa gcagatacag cgttttgttg atgcgcccca cttcaatgat
     5701 tgcctgagtc agtccggaag gacgttcact tttcagcaat gaacggacca gcactgaagc
     5761 ctgtactttg cccagcttca gggagccagc ggtccggatc atttcgtccc actgaaggac
     5821 tatttttcgg ggatctgatt gccctctggc aatatcattc agcacgccat agtcggcatc
     5881 atggtccatt cgccagaaaa ccgaagcacc ggcatcagcc aggcgtggag aaaactggta
     5941 tcccagcagc cagaaaaggc caaagacaag ttcgctggca cctgctgtat cggtcataat
     6001 ttcggttgga ttcagcccgg tctcctgttc cagaaggcct tccagcacaa agatagagtc
     6061 cctcagcgtc cccggtataa cgatgccatg aaagccggaa tactgatcgg acacaaagtt
     6121 gtaccaggtg atccctctgt tattaccaaa gtatttgcgg ttcggtccgg cattgattgt
     6181 tctgactggc gtaacaaagc gcattccatc tgcagatgcc acttctcctc caccccatat
     6241 ctgtgccagt ggcagcgttg cctgaaaatc aaccagtctg gcattagcgc tggtgatagt
     6301 ttcagcccgc agatagttcg cttttgtcca gttcagccgg tgtcgggtca gtgcaggaac
     6361 atttgatctg atcagtggtt ccagaccgat attgcaggct tcagccatca gcacggcgct
     6421 gatgctgacg ggcagatcat caactctggc actggcttca ctagcatgga aaaactcatc
     6481 agcaaatccg gtatgggcgt taatttcgag cagcaactcc gttaaatcca ccggagggag
     6541 cagatcactg atcattttgc tcagtcgttt cagactgtcc ggctcatcaa gactggcgag
     6601 gggagaaatt gtcaaccggg gcttcgggcc agaaacatcg agttcgacag cctcattttc
     6661 gccaagacgt gcagcaacct gtctgtaacg actatcaagc tgatgaccca gagattttat
     6721 tgcttcctgc gggtctgtcg ggtgtcccag agaacgataa accttaatcc ggtttgcctg
     6781 ccagtcagca ccctgtagta atcttgctcg gggatctccc caccggttac tgccggtaac
     6841 gtagacatcc ctccgcctca gactatcctg cagtttactg agaaagcaga gcgtgtatcc
     6901 cctgcgggtg atatgttttt ccttgttaat caccagccgt ttccatgacc gactgataat
     6961 ttccgttggt gcgtcgtcaa aaaactgccg ccgtgagctg aactcccggc tgaggtagtc
     7021 acaggcattc agagtggtaa ccccggcagg tgcggatgaa aatttaacgg tattcagcag
     7081 atggggcagg aaacgacgaa cgcgcccgta ctgctccacc atttcttcat gaaaattatc
     7141 gtctgagggc cgggcaattt cacggacaag cgtgatgatt tcagccagct tttgcctggg
     7201 gatgtagctg aacacctcag cacgaatcga ttcgtccggt gtttcttctt tcagcaggta
     7261 cgaacatgcg ctggcgagcg ccaatgcaga tttatccaga tccttcagcg agcggagccg
     7321 ttttttctgc ccaatctttc tggcgtcacg gatgataacg gccagcatgg cgtccagaac
     7381 gtccagtgca tcatccagcg ccagcgtttc ccatgcaagg acaaaggcaa ccagaaccgc
     7441 catccttttc tgcggtgaca tcctggcaat attgaacacc gaagtcatac cagcataacg
     7501 tgcgagattt ttcaggcgca cagccgggag tgtactcagg ttgtcagcat gcaagccaaa
     7561 atcgttcaga gttttccagc gttcaattgc ttcattaaac gccggaccac tgatggtcac
     7621 agggcctttt ttcagtgatt ccagtaaaga caggcggctg caatcagttg gccccagcag
     7681 catctccagc tgtgaacgct gttcggctga cggtatcagt gccagtttgt tccacaggcg
     7741 caacgtcgcc ttttccctta cctctgaaat caaccgagtc agcgtagtgg ctccggggag
     7801 aataatacga tgttgcataa gccaccctgt cgccagatcg aaaagcaggc caggacgttc
     7861 gttgcttatc cagctccggg tatataaaag acgggtaagg cgaaatgtcc agggccaggc
     7921 aaattcacga tactgatagt gctgacgtat cagcgctgca tgctcacggc gggtattttc
     7981 cctctgaccg tattctgcaa gaacggtgat atcacgaatc ccgagctgtc tggcggtaaa
     8041 atgccggacg ccggaaggaa tatgattcat atcggtgagg aaggtgccca gaaaacggac
     8101 acatccaatt tgcagggcaa tgcccagacg gttgtgatca cctctgcttt ttccgataaa
     8161 ttccttgtct gcttcatcaa ggtgaaaata tcgtgccagc tgaagctcat ccggttcacc
     8221 ggtgaatctg ccatagcttt cagtctgctc agtggtcaga aagtcaacgg gcatataggc
     8281 ctccctgcct tacgggcatt tagtaacatt tttgcaaccg tccgaaatgt tataaattat
     8341 cagacatagt aaaacggctt cgtttgagtg tccattaaat cgtcattttg gcataataga
     8401 cacatcgtgt ctgatattcg atttaaggta cattttatgc gaatttttgg ttatgcgcgg
     8461 gtctcaacca gccagcagtc cctcgatatt cagatcagag cgctcaaaga tgcaggggta
     8521 aaagctaacc gcatctttac cgacaaggca tccggcagtt caacagatcg ggaagggctg
     8581 gatttgctga ggatgaaggt ggaggaaggt gatgtcattc tggtgaagaa gctcgaccgt
     8641 cttggccgcg acaccgccga catgatccaa ctgataaaag agtttgatgc tcagggtgtc
     8701 gcggttcggt ttattgacga cgggatcagt accgacggtg atatggggca aatggtggtc
     8761 accatcctgt cggctgtggc acaagctgaa cgccggagga tcctagagcg cacgaatgag
     8821 ggccgacagg aagcaaagct gaaaggaatc aaatttggcc gcaggcgtac cgtggacagg
     8881 aacgtcgtgc tgacgcttca tcagaagggc actggtgcaa cggaaattgc tcatcagctc
     8941 agtattgccc gctccacggt ttataaaatt cttgaagacg aaagggcctc gtgatacgcc
     9001 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     9061 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     9121 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     9181 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     9241 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     9301 tgggttacat cgagctggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     9361 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggtgcggta ttatcccgtg
     9421 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     9481 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     9541 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     9601 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaacc cgccttgatc
     9661 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     9721 cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     9781 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     9841 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt ggatctcgcg
     9901 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     9961 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
    10021 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
    10081 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
    10141 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgacgtg tatttcttcg
    10201 caaatggcgt ggcatcgatg agttcccgga cttcttccgg attaccctga agcaccgttg
    10261 cgccttcgcg gttacgctcc ctccccagca ggtaatcaac cggaccactg ccaccacctt
    10321 ttcccctggc atgaaattta actatcatcc cgcgccccct gttccctgac agccagacgc
    10381 agccggcgca gctcatcccc gatggccatc agtgcggcca ccacctgaac ccggtcaccg
    10441 gaagaccact gcccgctgtt caccttacgg gctgtctgat tcaggttatt tccgatggcg
    10501 gccagctgac gcagtaacgg cggtgccagt gtcggcagtt ttccggaacg ggcaaccggc
    10561 tcccccagac agacccgccg catccatacc gccagttgtt taccctcaca gcgttcaagt
    10621 aaccgggcat gttcatcatc agtaacccgt attgtgagca tcctctcgcg tttcatcggt
    10681 atcattaccc catgaacaga aatccccctt acacggaggc atcagtgact aaacaggaaa
    10741 aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctg ctggagaagc
    10801 tcaacgaact ggacgcagat gaacaggccg atatttgtga atcgcttcac gaccacgccg
    10861 atgagcttta ccgcagctgc ctcgcacgtt tcggggatga cggtgaaaac ctctgacaca
    10921 tgcagctccc ggagacggtc acagcttgtc tgtgagcgga tgccgggagc tgacaagccc
    10981 gtcaggggcg tcagcaggtt ttagcgggtg tcggggcgca gccctgaccc agtcacgtag
    11041 cgatagcgga gtgtatactg gcttaaccat gcggcatcag tgcggattgt atgaaaagta
    11101 cgccatgccg ggtgtgaaat gccgcacaga tgcgtaagga gaaaatgcac gtccaggcgc
    11161 ttttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggta
    11221 ctgactcaca caaaaacggt aacacagtta tccacagaat caggggataa ggccggaaag
    11281 aacatgtgag caaaagacca ggaacaggaa gaaggccacg tagcaggcgt ttttccatag
    11341 gctccgcccc cctgacgagc atcacaaaaa tagacgctca agtcagaggt ggcgaaaccc
    11401 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
    11461 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
    11521 ttctcatagc tcacgctgtt ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
    11581 ctgtgtgcac gaacccccc
//
