LOCUS       NZ_CP120525             5103 bp    DNA     circular CON 08-APR-2024
DEFINITION  Enterobacter roggenkampii strain 0-E plasmid pER0-E4, complete
            sequence.
ACCESSION   NZ_CP120525
VERSION     NZ_CP120525.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33770999
            Assembly: GCF_029536245.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 5103)
  AUTHORS   Gauthier,J., Kukavica-Ibrulj,I., Cockenpot,L., Mohit,V., Brisson,C.
            and Levesque,R.C.
  TITLE     Complete Genome Sequence of Enterobacter roggenkampii 0-E
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5103)
  AUTHORS   Gauthier,J., Kukavica-Ibrulj,I., Cockenpot,L., Mohit,V., Brisson,C.
            and Levesque,R.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAR-2023) Institut de Biologie Integrative et des
            Systemes (IBIS), Universite Laval, 1030, avenue de la Medecine,
            Quebec City, QC G1V0A6, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 04-JUL-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Assembly Name                     :: UL_Erog_v1.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 26.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029536245.1-RS_2024_04_08
            Annotation Date                   :: 04/08/2024 17:05:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,862
            CDSs (total)                      :: 4,747
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 156 of 234
            Pseudo Genes (incomplete)         :: 84 of 234
            Pseudo Genes (internal stop)      :: 25 of 234
            Pseudo Genes (multiple problems)  :: 27 of 234
            Pseudo Genes (short protein)      :: 1 of 234
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP120525.1.
            Bacteria and source DNA available from Institute of integrative and
            systems biology, Laval University, Quebec, Canada.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5103
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="0-E"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1812935"
                     /plasmid="pER0-E4"
                     /geo_loc_name="Canada: Quebec"
                     /lat_lon="46.8131 N 71.2075 W"
                     /collection_date="2022"
                     /collected_by="MELCCFFP"
     gene            complement(join(5100..5103,1..170))
                     /locus_tag="P3S52_RS24240"
                     /old_locus_tag="P3S52_24275"
     CDS             complement(join(5100..5103,1..170))
                     /locus_tag="P3S52_RS24240"
                     /old_locus_tag="P3S52_24275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_277592342.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1514..2083
                     /locus_tag="P3S52_RS24245"
                     /old_locus_tag="P3S52_24280"
     CDS             1514..2083
                     /locus_tag="P3S52_RS24245"
                     /old_locus_tag="P3S52_24280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_085843690.1"
                     /translation="MAKLPLPLPIASPPTVNNSGSERAKKSGRRTLAAKRKTLLEELWG
                     DELEQLKVWNRHEHDGYTTVPRTLTYISRILDYLGGTGLPLSQTYLALWCRVFDEGFVE
                     IRDKDGFAYEAGFSGQRAVTTWTGRMRKLRDLGFITTKPGTSGEFQYVILLNPLTVIKE
                     LYEGKEKDERYNALVGRMQEVGAKWE"
                     /name="hypothetical protein"
     gene            2087..2632
                     /locus_tag="P3S52_RS24250"
                     /old_locus_tag="P3S52_24285"
     CDS             2087..2632
                     /locus_tag="P3S52_RS24250"
                     /old_locus_tag="P3S52_24285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010450637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_009310050.1"
                     /translation="MASYFPTLDISGEEFDFTHLEPFIMHVNSDMAKKVLKVHVRFTNH
                     CFTKGYEAFYHPAGEQIIYDHSGNGRTFCPIRYRLSLALPEIINRLCDPAEKVYQTSAK
                     RNWAYSITIDDPEGPYHLFFEIRRASRDEKHLQDLNMVIESAYPEDRGYGPPSLLGRIG
                     FVGLCGRVFCGKPLATKR"
                     /name="hypothetical protein"
     gene            complement(3928..4329)
                     /locus_tag="P3S52_RS24255"
                     /old_locus_tag="P3S52_24290"
     CDS             complement(3928..4329)
                     /locus_tag="P3S52_RS24255"
                     /old_locus_tag="P3S52_24290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_087840457.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGSFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTEFPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(4329..4520)
                     /locus_tag="P3S52_RS24260"
                     /old_locus_tag="P3S52_24295"
     CDS             complement(4329..4520)
                     /locus_tag="P3S52_RS24260"
                     /old_locus_tag="P3S52_24295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          292..695
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.827"
                     /coverage="99.0"
                     /evalue="4.5799999999999997e-172"
     Typing          4642..5045
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.827"
                     /coverage="99.0"
                     /evalue="4.5799999999999997e-172"
ORIGIN
        1 tccagtctcg aggacagtgt acggaaaaga tgctccgcat catcgtggag tttctcacaa
       61 aggtcggcct cgacatcgag atcaagctcg ttaagcttct ccagcaggag cagagactga
      121 ttctggatga attttgccat attcagcgct gttttctgtt gtttcgtcat aagaggcctc
      181 acagcgtcag tgctcagtgt agggatgttc tcttttccgc ttcctcgctc actcagtcgc
      241 tacgctcctg ccgttcggct gcggcgagcg gcaggagcat gcgtacaaaa gctcgagaaa
      301 accgagcaat cgcgtgatgg ggaaaaggaa aggccgcggc gttgccgttt ttccataggc
      361 tccgcccccc tgacaagcat cacagaatct gacgctcaaa tcagtggtgg cgaaacccga
      421 caggactata aagataccag gcgtttcccc ctgatggctc cctcgtgcgc tctcctgttc
      481 ctgccattcg gtttgccggt gtcattccgc tgttatggcc gcgtttatct cattccacgc
      541 cagacactca gttccgggaa ggcagttcgc tccaagctgg actgtctgca cgaacccccc
      601 gttcagtccg accgctgcgc cttaaccggt aactatcatc ttgagtccaa cccggaaaga
      661 cacgacaaag cgccactggc agcagccatt ggtaagagtc aatgtgcgag agattttaga
      721 gagtacttga agtgatggcc ggactacagc tacactgaaa gaacagtttt ggtgactcgt
      781 ctcgcacaag acagttacct ggttaagcag ttccccaact aacttaacct tcgaaaaaac
      841 cacctcccca ggtggttttt tcgttttcag agcaagagat tacgacgata caaatgggat
      901 ctcaagaaga tccttaacta attgcagctc gcttcactat cacattcaac tcccgtcacc
      961 gtcgattaaa accatataca actacggtca ggtgattccg ctcactggag cgaaattacc
     1021 cgttaagctc aaaacgctta acgtaggata attcccgttt ccaagctaac cccatcaaca
     1081 tccacacata gctctcagac gcgctcaggc aagagccaag tcatttcctg ccccaaacca
     1141 caccataacc ataaaaacca ccgaaaatcg aacacagggc actatgagca ggcatatcga
     1201 tttgcattaa atggctgccg taaaattatt accttgcccc cttgcatccc cattcagcac
     1261 cgcgagcaca ttcagcacat tgagcacaat taagcacagc gagcacagaa acctgtcata
     1321 actcgttgaa tatcagtcac catatgctgt atttattaca actcacagat tgagttgtaa
     1381 taaatacata ctcacaagat acctatacgt tgaaaggcaa catcacaacc acacgcaaaa
     1441 cacaacataa ctcattgttt taaaagaaaa aacacttaaa ataaccgtat gttaaatgat
     1501 tttggagaat ataatggcga agttaccact gccactaccg atagcgtccc ccccaacagt
     1561 aaacaatagt ggctcagaaa gagcgaagaa gtcaggaaga agaactcttg cagcaaaaag
     1621 gaaaaccttg ttagaagaac tatggggaga cgaacttgaa caactcaagg tatggaatcg
     1681 ccatgagcat gacgggtata caactgtgcc aagaacgctg acatatatca gtcgaattct
     1741 cgattatctc ggaggaacgg gactgccatt gtcccaaaca tatctggctc tttggtgcag
     1801 ggtatttgat gagggctttg tggaaataag agataaagat ggatttgctt atgaagctgg
     1861 ttttagtgga cagcgagcag taaccacatg gacagggagg atgagaaaac taagagattt
     1921 aggtttcata actacaaaac ctggcacatc aggtgagttt caatatgtga ttttgcttaa
     1981 cccactgact gtcattaagg agttatacga aggtaaggaa aaagatgaaa gatacaatgc
     2041 attagtgggc cgtatgcagg aggttggtgc taaatgggag taattcatgg ctagctattt
     2101 cccgacgtta gacatatctg gagaagagtt cgatttcact caccttgagc cattcattat
     2161 gcacgtaaat agcgatatgg ctaaaaaggt attaaaagta cacgttaggt tcacaaatca
     2221 ttgttttaca aaaggttatg aagcttttta tcacccagca ggagaacaga taatttacga
     2281 ccatagcggg aatgggagaa cgttttgccc aattagatat agattatctt tggcattacc
     2341 tgagattatc aaccgtctat gcgatccagc agaaaaggtg tatcaaacat cagcaaagag
     2401 aaactgggcc tactcgatta cgattgatga tccagagggg ccatatcact tattttttga
     2461 aataagacgc gcctcgcgtg atgagaaaca cctacaagat ttgaacatgg ttatcgagag
     2521 tgcctatcct gaagatcgag ggtacgggcc tccgtcctta ctagggcgaa ttggctttgt
     2581 agggttatgc ggtagggtat tttgtgggaa gcctctggcg actaaaagat gaaaaaaaag
     2641 cacctgaaag gtgccttttt tctgtttctt tggtaatcgt tccggacacc actagctttt
     2701 gcttaggttc gccttgcgcg aacacaggaa caagatttgt cctgctgggt agcgctcaat
     2761 ctcatccggt cagtcgctac gatgtaaaca atataacaaa accaataacg caattcagca
     2821 ccaccggttc aatgcaatgc tttgattctt atggagtacc atgagtacag aaaccataag
     2881 taaccatctg aatttaaatg gcaagatgca ttttataaaa caactcaact tatgagttgt
     2941 tttataaaat gcatctattc ttatgattca ccatgatttc actacaatag aaaaaaacat
     3001 agatagttgt tttcattaaa aaaataaatc ccctcctgtt ttcatcacct tgatgcattg
     3061 gcaatcgtcg cacggtagct ttcactgata ccctcagagg gagctgccga acaggtcgcc
     3121 gggaggcggc ccgtttcgtc ccttagcagg aaaaccttca ggccccttcg gggctttttt
     3181 gcatctgtca tttaacataa aggcatgatt gtacgcaccg ctaaaacggc ctcagggcgt
     3241 ttccgacggg aaaccggtaa aacaggcaac aaaccacccg aaaacccgcc agaaacgcgc
     3301 aggctctgtt tttacggcat gcatgagtat gcacgcaagt gcatagcgcg aaaattcacg
     3361 ccattcagcc agggtaagtc ctgtttccgg cgttcttcct atccgaaaac ggtgaacggg
     3421 gacgcaattt attgcgtggc cgtgaagcgt tgccggcgca gccggaaaac cggttttcag
     3481 cacgatcagg aatcgggttc gtcgacggcc ggcaatcacg ttatggcacg tcgccggacg
     3541 actcaccaca cgattcaccg gcgattcgac tcgtgacttg actctagagt cgtcaaggtc
     3601 aaaaaaacac caaagtgacg attcgactct agagtcaagt cgtcataagt tactgatagt
     3661 cttaagtgag tcgtcgatga ctctatgacg agtcgtcggc aagtcgtaca cgaatcgccg
     3721 acgacttgga ttcaccttac agaaagtaat actgcgtaat ttaataaccc acaactaact
     3781 gatattaatg agttatcttc aaaattttaa ttttgcgtgg ggtgtgggta tttttagtgg
     3841 tgaggagcgt tcagagatct gacgattttt gggaaggttc aggaactccc cgggatggcc
     3901 ggagaggaaa actgaagcat gacggggtca ggattttttc cagaatttcc accatggcgc
     3961 cgggaattca gttaattttg cctggtgatt ttcaagaagg agcatggcct gtttcaggct
     4021 gtcaatgtgc gaatccttgg acaaaagtaa ggtgttcagg tgttcaatct cctgtttcag
     4081 atgacgaatc acctgttcac tctggtcagc agaggctgta tcacgctgtt cagagacctg
     4141 tccagaatgg tgttcaccct tcggttgctc aaaggaacca aagacacgta aaagctctga
     4201 agtatctacc atttttgcat tacgttcacc gctacaggtg gacaggtcac ctgctttaat
     4261 taacctgtgc agtgttgttc tactcttacc cgtcagtcgg gcagcctcac taatagacac
     4321 tctggccatc agttaccatc ctgtaatgca tccagtctcg aggacagtgt acggaaaaga
     4381 tgctccgcat catcgtggag tttctcacaa aggtcggcct cgacatcgag atcaagctcg
     4441 ttaagcttct ccagcaggag cagagactga ttctggatga attttgccat attcagcgct
     4501 gttttctgtt gtttcgtcat aagaggcctc acagcgtcag tgctcagtgt agggatgttc
     4561 tcttttccgc ttcctcgctc actcagtcgc tacgctcctg ccgttcggct gcggcgagcg
     4621 gcaggagcat gcgtacaaaa gctcgagaaa accgagcaat cgcgtgatgg ggaaaaggaa
     4681 aggccgcggc gttgccgttt ttccataggc tccgcccccc tgacaagcat cacagaatct
     4741 gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     4801 ctgatggctc cctcgtgcgc tctcctgttc ctgccattcg gtttgccggt gtcattccgc
     4861 tgttatggcc gcgtttatct cattccacgc cagacactca gttccgggaa ggcagttcgc
     4921 tccaagctgg actgtctgca cgaacccccc gttcagtccg accgctgcgc cttaaccggt
     4981 aactatcatc ttgagtccaa cccggaaaga cacgacaaag cgccactggc agcagccatt
     5041 ggtaagagtc aatgtgcgag agattttaga gagtacttga agtgatggcc ggactacagc
     5101 tac
//
