LOCUS       NZ_CP120203             3128 bp    DNA     circular CON 06-JAN-2024
DEFINITION  Staphylococcus aureus strain Dog002_mouth plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP120203
VERSION     NZ_CP120203.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33696749
            Assembly: GCF_034020215.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Canine Staphylococcaceae circulating in a Kenyan animal shelter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
  REMARK    Annotation added by the database at the request of the submitter
REFERENCE   3  (bases 1 to 3128)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2022
            Assembly Method                   :: Flye v. v2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 320x
            Sequencing Technology             :: PacBio Sequel II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/06/2024 10:57:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,752
            Genes (coding)                    :: 2,665
            CDSs (with protein)               :: 2,665
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 46 of 87
            Pseudo Genes (incomplete)         :: 35 of 87
            Pseudo Genes (internal stop)      :: 21 of 87
            Pseudo Genes (multiple problems)  :: 15 of 87
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP120203.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="Dog002_mouth"
                     /isolation_source="mouth swab"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed2"
                     /geo_loc_name="Kenya:Nairobi"
                     /lat_lon="1.3345 S 36.7331 E"
                     /collection_date="2015-06-27"
                     /collected_by="Joerg Jores, Anna Lacasta, Anne Liljander,
                     Paul Ssajjakambwe (International Livestock Research
                     Institute, ILRI)"
     gene            join(3040..3128,1..102)
                     /locus_tag="P3U42_RS14160"
                     /old_locus_tag="P3U42_14160"
                     /pseudo=""
     CDS             join(3040..3128,1..102)
                     /locus_tag="P3U42_RS14160"
                     /old_locus_tag="P3U42_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(428..1009)
                     /locus_tag="P3U42_RS14165"
                     /old_locus_tag="P3U42_14165"
     CDS             complement(428..1009)
                     /locus_tag="P3U42_RS14165"
                     /old_locus_tag="P3U42_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000991705.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIAD
                     KNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDIEN
                     KKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVAKKL
                     AKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
                     /name="hypothetical protein"
     gene            1461..2456
                     /locus_tag="P3U42_RS14170"
                     /old_locus_tag="P3U42_14170"
     CDS             1461..2456
                     /locus_tag="P3U42_RS14170"
                     /old_locus_tag="P3U42_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001199892.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     Typing          2527..2691
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.42e-81"
     Typing          1462..2456
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.9"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 catttggaaa atttatatat attaattccg tatgttattt tgatgggaat aaatgctatt
       61 tatttggttg ttaaggttat gaatttcaaa aagaataatt agaatatttg aggggatttt
      121 gaaacgagtt tcttcttgtt ttcacactgt ttttttattc ctattggtgt tgttgcttac
      181 tttttgtttt tctataaaga tgatgctttt gacgagatgg aagaaaaata tgattatcat
      241 gaagataata aaaaatagac gacgcattta tgccgagaaa atttattgat gttgagaaga
      301 acccttaact aaacttgcag acgaatgtcg gcatagcgtg agctattaag ccgaccatgc
      361 gacaagtttt gggattgtta agggttccga ggctcaacgt caataaagca attggaataa
      421 atagtatcta aagtaacccg tatttaagat tacattgtac atccttcgaa accatagtag
      481 ctgcgattcc agctaggttt ccttttatcc caattttagc taattttttt gctacgccct
      541 ttaagttttt ggctttaaca tcttcaaaaa ctgctgtgat tatgtttcca gttaaaagtt
      601 ctccatatga gtttaaaact tcagaataga aacagtcacc gctatttttg tagttcccac
      661 caactgctct aggtgatatt gaactagttt tctttttatt ctctatgtcg ttttttagtt
      721 tttggaatcc ttcaggaaca tatccatatc gagatttggc attttctaga ttaacatctt
      781 tagctgttcc gtctttatct aaaataataa ttttgccata tatgtatttt agttcttttt
      841 caatttcttt ttcgtttaaa gtttcaacgt ttttatctgc aatgttattt ttgttttggg
      901 cttgagcttc tttgaaatta aaatccaaga ccattgaaag tatggttaaa atacttaaag
      961 atactactaa aatctttctt agtgatatac tgttttgaat tgcattcatt gtaaatgccc
     1021 ccttgttttg ttatttaaat gaattcaata taatattaac atataaaaac aaaggagttt
     1081 aatatgaata agttttttta ctttttgatt attttattat ctgtattttt aatttttaat
     1141 tttgttaatt cgattttatt tgataatcct tttgactttt tagaaagttt tatttattca
     1201 ttggttttca cattaattta tttaggttta gagggtcata aaaagaagaa ggaataggtt
     1261 gttttttgaa acgagtgtga acgagtttct tcttgtcttg atactatata gaaataactc
     1321 gattttatat atatagctgt aactgttgat attacagtgt ttaaacgtgt ttttgtgcgt
     1381 gaaaggaaaa tttgacaata aaaaacccca gttatattat taaggtgtcg aatcttaaat
     1441 aatactgggg gtctttttat atgcaatata atactactag aagtataacc gaaaatcaag
     1501 ataataaaac gttaaaagat atgacgaaaa gtgggaaaca acgcccatgg agagaaaaga
     1561 aaatagataa tgtaagctat gcagatatac tagaaatttt aaaaatcaaa aaggctttta
     1621 atgtaaaaca atgtggtaat attttagaat ttaagccaac tgatgaaggc tatttgaagt
     1681 tacataagac atggttttgt aaatcaaaat tatgtccggt ttgtaattgg agacgtgcta
     1741 tgaaaaatag ttatcaagct caaaaagtga ttgaaaaagt aattaaggaa aagccaaaag
     1801 cacgttggtt gtttttaaca ctttcaacaa aaaatgcgat agatggagat actttagaac
     1861 aaagtttgaa gcatctaact aaagcatttg ataggttgag tagatataaa aaggttaaac
     1921 aaaatcttgt tggatttatg cgttcaacag aagttaccgt taataaaaat gacggtagtt
     1981 ataatcagca catgcatgtt ttgttatgtg ttgaaaatgc atattttaga aaaaaagaga
     2041 attatataac tcaagaagaa tgggttaatt tatggcaaag agcattacaa gttgactatc
     2101 gacctgttgc taatgttaaa gcgatcaaac cgaatagaaa aggcgataaa gacattgaat
     2161 cggcaatcaa agagacctca aaatattcgg ttaaatcatc tgatttttta actgatgatg
     2221 atgaaaaaaa tcaagaaatt gtaagtgatt tagaaaaagg tttgtatcga aaacgtatgt
     2281 taagttatgg tggattgctt aaacaaaaac ataaaatttt aaacttagac gatgtcgaag
     2341 atggtaattt gattaatgca agtgatgaag ataaaacaac agacgaagaa gaaaaagcac
     2401 attcaattac cgcaatttgg aatttcgaaa agcaaaatta ttatttaaga cattagtgtt
     2461 gactaatgtc ttttttgttg attttttata aaaaagtact gtcttatttt tgtgacaaat
     2521 gctgtatgta gtgtcacaaa aataagacaa acgcaatata ttgtgtcaca aaaataagac
     2581 agtacagctt tgtatgatat cactttaaaa taccttaaaa cccctggaat gtctggcttt
     2641 gccagaccta tcatttttga atgatagcaa attctcctta tgctcttacg gagtttttag
     2701 agaaaaatta aaaattctcg atttttgata aaaaacgccc tgcaggaatt tagaaaaaca
     2761 tgtggaagtt ttaaaggatt ttatgctaat ttttaatttg catgtaaatg ggcagtgtta
     2821 aaaaattagt cactggtttt gctgaaattt ttgtttgtga aattaccata tatttatttg
     2881 gctcatattt gcgttttaag agcttgtata tatttttaag ttaaaaacct atatgatacc
     2941 cccctaaacc tttaaaatac ccccttaaaa ctcaaaataa aggtatttat aatttgagta
     3001 agtcttgtga taaagttttt taaaaaagga gtgtttattt tgaataaaaa cacagtgtta
     3061 gatgaaggtt acgttatagc aactatttta aatgttttct ttttacttgg attaattttt
     3121 atatctca
//
