LOCUS       NZ_CP120184             3152 bp    DNA     circular CON 06-JAN-2024
DEFINITION  Staphylococcus aureus strain Dog014 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP120184
VERSION     NZ_CP120184.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33696760
            Assembly: GCF_034016595.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Canine Staphylococcaceae circulating in a Kenyan animal shelter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
  REMARK    Annotation added by database at the request of the submitter
REFERENCE   3  (bases 1 to 3152)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2022
            Assembly Method                   :: Flye v. v2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 199x
            Sequencing Technology             :: PacBio Sequel II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/06/2024 10:55:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,829
            CDSs (total)                      :: 2,747
            Genes (coding)                    :: 2,660
            CDSs (with protein)               :: 2,660
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 46 of 87
            Pseudo Genes (incomplete)         :: 35 of 87
            Pseudo Genes (internal stop)      :: 21 of 87
            Pseudo Genes (multiple problems)  :: 15 of 87
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP120184.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="Dog014"
                     /isolation_source="nasal swab"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed2"
                     /geo_loc_name="Kenya:Nairobi"
                     /lat_lon="1.3345 S 36.7331 E"
                     /collection_date="2015-06-27"
                     /collected_by="Joerg Jores, Anna Lacasta, Anne Liljander,
                     Paul Ssajjakambwe (International Livestock Research
                     Institute, ILRI)"
     gene            126..1121
                     /locus_tag="P3U66_RS14135"
                     /old_locus_tag="P3U66_14135"
     CDS             126..1121
                     /locus_tag="P3U66_RS14135"
                     /old_locus_tag="P3U66_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001199892.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     gene            1705..1893
                     /locus_tag="P3U66_RS14140"
                     /old_locus_tag="P3U66_14140"
     CDS             1705..1893
                     /locus_tag="P3U66_RS14140"
                     /old_locus_tag="P3U66_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001039248.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     gene            complement(2219..2800)
                     /locus_tag="P3U66_RS14145"
                     /old_locus_tag="P3U66_14145"
     CDS             complement(2219..2800)
                     /locus_tag="P3U66_RS14145"
                     /old_locus_tag="P3U66_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000991705.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIAD
                     KNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDIEN
                     KKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVAKKL
                     AKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
                     /name="hypothetical protein"
     Typing          1192..1356
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.42e-81"
     Typing          127..1121
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.9"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gctgtaactg ttgatattac agtgtttaaa cgtgtttttg tgcgtgaaag gaaaatttga
       61 caataaaaaa ccccagttat attattaagg tgtcgaatct taaataatac tgggggtctt
      121 tttatatgca atataatact actagaagta taaccgaaaa tcaagataat aaaacgttaa
      181 aagatatgac gaaaagtggg aaacaacgcc catggagaga aaagaaaata gataatgtaa
      241 gctatgcaga tatactagaa attttaaaaa tcaaaaaggc ttttaatgta aaacaatgtg
      301 gtaatatttt agaatttaag ccaactgatg aaggctattt gaagttacat aagacatggt
      361 tttgtaaatc aaaattatgt ccggtttgta attggagacg tgctatgaaa aatagttatc
      421 aagctcaaaa agtgattgaa aaagtaatta aggaaaagcc aaaagcacgt tggttgtttt
      481 taacactttc aacaaaaaat gcgatagatg gagatacttt agaacaaagt ttgaagcatc
      541 taactaaagc atttgatagg ttgagtagat ataaaaaggt taaacaaaat cttgttggat
      601 ttatgcgttc aacagaagtt accgttaata aaaatgacgg tagttataat cagcacatgc
      661 atgttttgtt atgtgttgaa aatgcatatt ttagaaaaaa agagaattat ataactcaag
      721 aagaatgggt taatttatgg caaagagcat tacaagttga ctatcgacct gttgctaatg
      781 ttaaagcgat caaaccgaat agaaaaggcg ataaagacat tgaatcggca atcaaagaga
      841 cctcaaaata ttcggttaaa tcatctgatt ttttaactga tgatgatgaa aaaaatcaag
      901 aaattgtaag tgatttagaa aaaggtttgt atcgaaaacg tatgttaagt tatggtggat
      961 tgcttaaaca aaaacataaa attttaaact tagacgatgt cgaagatggt aatttgatta
     1021 atgcaagtga tgaagataaa acaacagacg aagaagaaaa agcacattca attaccgcaa
     1081 tttggaattt cgaaaagcaa aattattatt taagacatta gtgttgacta atgtcttttt
     1141 tgttgatttt ttataaaaaa gtactgtctt atttttgtga caaatgctgt atgtagtgtc
     1201 acaaaaataa gacaaacgca atatattgtg tcacaaaaat aagacagtac agctttgtat
     1261 gatatcactt taaaatacct taaaacccct ggaatgtctg gctttgccag acctatcatt
     1321 tttgaatgat agcaaattct ccttatgctc ttacggagtt tttagagaaa aattaaaaat
     1381 tctcgatttt tgataaaaaa cgccctgcag gaatttagaa aaacatgtgg aagttttaaa
     1441 ggattttatg ctaattttta atttgcatgt aaatgggcag tgttaaaaaa ttagtcactg
     1501 gttttgctga aatttttgtt tgtgaaatta ccatatattt atttggctca tatttgcgtt
     1561 ttaagagctt gtatatattt ttaagttaaa aacctatatg atacccccct aaacctttaa
     1621 aataccccct taaaactcaa aataaaggta tttataattt gagtaagtct tgtgataaag
     1681 ttttttaaaa aaggagtgtt tattttgaat aaaaacacag tgttagatga aggttacgtt
     1741 atagcaacta ttttaaatgt tttcttttta cttggattaa tttttatatc tcatttggaa
     1801 aatttatata tattaattcc gtatgttatt ttgatgggaa taaatgctat ttatttggtt
     1861 gttaaggtta tgaatttcaa aaagaataat tagaatattt gaggggattt tgaaacgagt
     1921 ttcttcttgt tttcacactg tttttttatt cctattggtg ttgttgctta ctttttgttt
     1981 ttctataaag atgatgcttt tgacgagatg gaagaaaaat atgattatca tgaagataat
     2041 aaaaaataga cgacgcattt atgccgagaa aatttattga tgttgagaag aacccttaac
     2101 taaacttgca gacgaatgtc ggcatagcgt gagctattaa gccgaccatg cgacaagttt
     2161 tgggattgtt aagggttccg aggctcaacg tcaataaagc aattggaata aatagtatct
     2221 aaagtaaccc gtatttaaga ttacattgta catccttcga aaccatagta gctgcgattc
     2281 cagctaggtt tccttttatc ccaattttag ctaatttttt tgctacgccc tttaagtttt
     2341 tggctttaac atcttcaaaa actgctgtga ttatgtttcc agttaaaagt tctccatatg
     2401 agtttaaaac ttcagaatag aaacagtcac cgctattttt gtagttccca ccaactgctc
     2461 taggtgatat tgaactagtt ttctttttat tctctatgtc gttttttagt ttttggaatc
     2521 cttcaggaac atatccatat cgagatttgg cattttctag attaacatct ttagctgttc
     2581 cgtctttatc taaaataata attttgccat atatgtattt tagttctttt tcaatttctt
     2641 tttcgtttaa agtttcaacg tttttatctg caatgttatt tttgttttgg gcttgagctt
     2701 ctttgaaatt aaaatccaag accattgaaa gtatggttaa aatacttaaa gatactacta
     2761 aaatctttct tagtgatata ctgttttgaa ttgcattcat tgtaaatgcc cccttgtttt
     2821 gttatttaaa tgaattcaat ataatattaa catataaaaa caaaggagtt taatatgaat
     2881 aagttttttt actttttgat tattttatta tctgtatttt taatttttaa ttttgttaat
     2941 tcgattttat ttgataatcc ttttgacttt ttagaaagtt ttatttattc attggttttc
     3001 acattaattt atttaggttt agagggtcat aaaaagaaga aggaataggt tgttttttga
     3061 aacgagtgtg aacgagtttc ttcttgtctt gatactatat agaaataact cgattttata
     3121 tatatagctg taactgttga tattacagtg tt
//
