LOCUS       NZ_CP120176             3177 bp    DNA     circular CON 06-JAN-2024
DEFINITION  Staphylococcus aureus strain Dog020 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP120176
VERSION     NZ_CP120176.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33696765
            Assembly: GCF_034014575.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Canine Staphylococcaceae circulating in a Kenyan animal shelter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
  REMARK    Annotation added by database at the request of the submitter
REFERENCE   3  (bases 1 to 3177)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2022
            Assembly Method                   :: Flye v. v2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 260x
            Sequencing Technology             :: PacBio Sequel II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/06/2024 10:50:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,829
            CDSs (total)                      :: 2,747
            Genes (coding)                    :: 2,661
            CDSs (with protein)               :: 2,661
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 45 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 21 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP120176.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="Dog020"
                     /isolation_source="nasal swab"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed2"
                     /geo_loc_name="Kenya:Nairobi"
                     /lat_lon="1.3345 S 36.7331 E"
                     /collection_date="2015-06-27"
                     /collected_by="Joerg Jores, Anna Lacasta, Anne Liljander,
                     Paul Ssajjakambwe (International Livestock Research
                     Institute, ILRI)"
     gene            complement(190..1185)
                     /locus_tag="P3T95_RS14135"
                     /old_locus_tag="P3T95_14135"
     CDS             complement(190..1185)
                     /locus_tag="P3T95_RS14135"
                     /old_locus_tag="P3T95_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001199892.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     gene            1637..2218
                     /locus_tag="P3T95_RS14140"
                     /old_locus_tag="P3T95_14140"
     CDS             1637..2218
                     /locus_tag="P3T95_RS14140"
                     /old_locus_tag="P3T95_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000991705.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIAD
                     KNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDIEN
                     KKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVAKKL
                     AKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
                     /name="hypothetical protein"
     gene            complement(2544..2732)
                     /locus_tag="P3T95_RS14145"
                     /old_locus_tag="P3T95_14145"
     CDS             complement(2544..2732)
                     /locus_tag="P3T95_RS14145"
                     /old_locus_tag="P3T95_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001039248.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     Typing          complement(2..120)
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="9.01e-56"
     Typing          complement(3082..3177)
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.499999999999999e-43"
     Typing          complement(191..1185)
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.9"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctggcaaagc cagacattcc aggggtttta aggtatttta aagtgatatc atacaaagct
       61 gtactgtctt atttttgtga cacaatatat tgcgtttgtc ttatttttgt gacactacat
      121 acagcatttg tcacaaaaat aagacagtac ttttttataa aaaatcaaca aaaaagacat
      181 tagtcaacac taatgtctta aataataatt ttgcttttcg aaattccaaa ttgcggtaat
      241 tgaatgtgct ttttcttctt cgtctgttgt tttatcttca tcacttgcat taatcaaatt
      301 accatcttcg acatcgtcta agtttaaaat tttatgtttt tgtttaagca atccaccata
      361 acttaacata cgttttcgat acaaaccttt ttctaaatca cttacaattt cttgattttt
      421 ttcatcatca tcagttaaaa aatcagatga tttaaccgaa tattttgagg tctctttgat
      481 tgccgattca atgtctttat cgccttttct attcggtttg atcgctttaa cattagcaac
      541 aggtcgatag tcaacttgta atgctctttg ccataaatta acccattctt cttgagttat
      601 ataattctct ttttttctaa aatatgcatt ttcaacacat aacaaaacat gcatgtgctg
      661 attataacta ccgtcatttt tattaacggt aacttctgtt gaacgcataa atccaacaag
      721 attttgttta acctttttat atctactcaa cctatcaaat gctttagtta gatgcttcaa
      781 actttgttct aaagtatctc catctatcgc attttttgtt gaaagtgtta aaaacaacca
      841 acgtgctttt ggcttttcct taattacttt ttcaatcact ttttgagctt gataactatt
      901 tttcatagca cgtctccaat tacaaaccgg acataatttt gatttacaaa accatgtctt
      961 atgtaacttc aaatagcctt catcagttgg cttaaattct aaaatattac cacattgttt
     1021 tacattaaaa gcctttttga tttttaaaat ttctagtata tctgcatagc ttacattatc
     1081 tattttcttt tctctccatg ggcgttgttt cccacttttc gtcatatctt ttaacgtttt
     1141 attatcttga ttttcggtta tacttctagt agtattatat tgcatataaa aagaccccca
     1201 gtattattta agattcgaca ccttaataat ataactgggg ttttttattg tcaaattttc
     1261 ctttcacgca caaaaacacg tttaaacact gtaatatcaa cagttacagc tatatatata
     1321 aaatcgagtt atttctatat agtatcaaga caagaagaaa ctcgttcaca ctcgtttcaa
     1381 aaaacaacct attccttctt ctttttatga ccctctaaac ctaaataaat taatgtgaaa
     1441 accaatgaat aaataaaact ttctaaaaag tcaaaaggat tatcaaataa aatcgaatta
     1501 acaaaattaa aaattaaaaa tacagataat aaaataatca aaaagtaaaa aaacttattc
     1561 atattaaact cctttgtttt tatatgttaa tattatattg aattcattta aataacaaaa
     1621 caagggggca tttacaatga atgcaattca aaacagtata tcactaagaa agattttagt
     1681 agtatcttta agtattttaa ccatactttc aatggtcttg gattttaatt tcaaagaagc
     1741 tcaagcccaa aacaaaaata acattgcaga taaaaacgtt gaaactttaa acgaaaaaga
     1801 aattgaaaaa gaactaaaat acatatatgg caaaattatt attttagata aagacggaac
     1861 agctaaagat gttaatctag aaaatgccaa atctcgatat ggatatgttc ctgaaggatt
     1921 ccaaaaacta aaaaacgaca tagagaataa aaagaaaact agttcaatat cacctagagc
     1981 agttggtggg aactacaaaa atagcggtga ctgtttctat tctgaagttt taaactcata
     2041 tggagaactt ttaactggaa acataatcac agcagttttt gaagatgtta aagccaaaaa
     2101 cttaaagggc gtagcaaaaa aattagctaa aattgggata aaaggaaacc tagctggaat
     2161 cgcagctact atggtttcga aggatgtaca atgtaatctt aaatacgggt tactttagat
     2221 actatttatt ccaattgctt tattgacgtt gagcctcgga acccttaaca atcccaaaac
     2281 ttgtcgcatg gtcggcttaa tagctcacgc tatgccgaca ttcgtctgca agtttagtta
     2341 agggttcttc tcaacatcaa taaattttct cggcataaat gcgtcgtcta ttttttatta
     2401 tcttcatgat aatcatattt ttcttccatc tcgtcaaaag catcatcttt atagaaaaac
     2461 aaaaagtaag caacaacacc aataggaata aaaaaacagt gtgaaaacaa gaagaaactc
     2521 gtttcaaaat cccctcaaat attctaatta ttctttttga aattcataac cttaacaacc
     2581 aaataaatag catttattcc catcaaaata acatacggaa ttaatatata taaattttcc
     2641 aaatgagata taaaaattaa tccaagtaaa aagaaaacat ttaaaatagt tgctataacg
     2701 taaccttcat ctaacactgt gtttttattc aaaataaaca ctcctttttt aaaaaacttt
     2761 atcacaagac ttactcaaat tataaatacc tttattttga gttttaaggg ggtattttaa
     2821 aggtttaggg gggtatcata taggttttta acttaaaaat atatacaagc tcttaaaacg
     2881 caaatatgag ccaaataaat atatggtaat ttcacaaaca aaaatttcag caaaaccagt
     2941 gactaatttt ttaacactgc ccatttacat gcaaattaaa aattagcata aaatccttta
     3001 aaacttccac atgtttttct aaattcctgc agggcgtttt ttatcaaaaa tcgagaattt
     3061 ttaatttttc tctaaaaact ccgtaagagc ataaggagaa tttgctatca ttcaaaaatg
     3121 ataggtctgg caaagccaga cattccaggg gttttaaggt attttaaagt gatatca
//
