LOCUS       NZ_CP120169             3139 bp    DNA     circular CON 06-JAN-2024
DEFINITION  Staphylococcus aureus strain Dog023 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP120169
VERSION     NZ_CP120169.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33696768
            Assembly: GCF_034013695.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Canine Staphylococcaceae circulating in a Kenyan animal shelter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
  REMARK    Annotation added by database at the request of the submitter
REFERENCE   3  (bases 1 to 3139)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2022
            Assembly Method                   :: Flye v. v2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 328x
            Sequencing Technology             :: PacBio Sequel II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/06/2024 10:56:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,824
            CDSs (total)                      :: 2,742
            Genes (coding)                    :: 2,656
            CDSs (with protein)               :: 2,656
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 45 of 86
            Pseudo Genes (incomplete)         :: 35 of 86
            Pseudo Genes (internal stop)      :: 21 of 86
            Pseudo Genes (multiple problems)  :: 15 of 86
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP120169.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="Dog023"
                     /isolation_source="nasal swab"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed2"
                     /geo_loc_name="Kenya:Nairobi"
                     /lat_lon="1.3345 S 36.7331 E"
                     /collection_date="2015-06-27"
                     /collected_by="Joerg Jores, Anna Lacasta, Anne Liljander,
                     Paul Ssajjakambwe (International Livestock Research
                     Institute, ILRI)"
     gene            202..1197
                     /locus_tag="P3U02_RS14110"
                     /old_locus_tag="P3U02_14110"
     CDS             202..1197
                     /locus_tag="P3U02_RS14110"
                     /old_locus_tag="P3U02_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001199892.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     gene            1781..1969
                     /locus_tag="P3U02_RS14115"
                     /old_locus_tag="P3U02_14115"
     CDS             1781..1969
                     /locus_tag="P3U02_RS14115"
                     /old_locus_tag="P3U02_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001039248.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     gene            complement(2295..2876)
                     /locus_tag="P3U02_RS14120"
                     /old_locus_tag="P3U02_14120"
     CDS             complement(2295..2876)
                     /locus_tag="P3U02_RS14120"
                     /old_locus_tag="P3U02_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000991705.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIAD
                     KNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDIEN
                     KKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVAKKL
                     AKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
                     /name="hypothetical protein"
     Typing          1268..1432
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.42e-81"
     Typing          203..1197
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.9"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggttttttga aacgagtgtg aacgagtttc ttcttgtctt gatactatat agaaataact
       61 cgattttata tatatagctg taactgttga tattacagtg tttaaacgtg tttttgtgcg
      121 tgaaaggaaa atttgacaat aaaaaacccc agttatatta ttaaggtgtc gaatcttaaa
      181 taatactggg ggtcttttta tatgcaatat aatactacta gaagtataac cgaaaatcaa
      241 gataataaaa cgttaaaaga tatgacgaaa agtgggaaac aacgcccatg gagagaaaag
      301 aaaatagata atgtaagcta tgcagatata ctagaaattt taaaaatcaa aaaggctttt
      361 aatgtaaaac aatgtggtaa tattttagaa tttaagccaa ctgatgaagg ctatttgaag
      421 ttacataaga catggttttg taaatcaaaa ttatgtccgg tttgtaattg gagacgtgct
      481 atgaaaaata gttatcaagc tcaaaaagtg attgaaaaag taattaagga aaagccaaaa
      541 gcacgttggt tgtttttaac actttcaaca aaaaatgcga tagatggaga tactttagaa
      601 caaagtttga agcatctaac taaagcattt gataggttga gtagatataa aaaggttaaa
      661 caaaatcttg ttggatttat gcgttcaaca gaagttaccg ttaataaaaa tgacggtagt
      721 tataatcagc acatgcatgt tttgttatgt gttgaaaatg catattttag aaaaaaagag
      781 aattatataa ctcaagaaga atgggttaat ttatggcaaa gagcattaca agttgactat
      841 cgacctgttg ctaatgttaa agcgatcaaa ccgaatagaa aaggcgataa agacattgaa
      901 tcggcaatca aagagacctc aaaatattcg gttaaatcat ctgatttttt aactgatgat
      961 gatgaaaaaa atcaagaaat tgtaagtgat ttagaaaaag gtttgtatcg aaaacgtatg
     1021 ttaagttatg gtggattgct taaacaaaaa cataaaattt taaacttaga cgatgtcgaa
     1081 gatggtaatt tgattaatgc aagtgatgaa gataaaacaa cagacgaaga agaaaaagca
     1141 cattcaatta ccgcaatttg gaatttcgaa aagcaaaatt attatttaag acattagtgt
     1201 tgactaatgt cttttttgtt gattttttat aaaaaagtac tgtcttattt ttgtgacaaa
     1261 tgctgtatgt agtgtcacaa aaataagaca aacgcaatat attgtgtcac aaaaataaga
     1321 cagtacagct ttgtatgata tcactttaaa ataccttaaa acccctggaa tgtctggctt
     1381 tgccagacct atcatttttg aatgatagca aattctcctt atgctcttac ggagttttta
     1441 gagaaaaatt aaaaattctc gatttttgat aaaaaacgcc ctgcaggaat ttagaaaaac
     1501 atgtggaagt tttaaaggat tttatgctaa tttttaattt gcatgtaaat gggcagtgtt
     1561 aaaaaattag tcactggttt tgctgaaatt tttgtttgtg aaattaccat atatttattt
     1621 ggctcatatt tgcgttttaa gagcttgtat atatttttaa gttaaaaacc tatatgatac
     1681 ccccctaaac ctttaaaata cccccttaaa actcaaaata aaggtattta taatttgagt
     1741 aagtcttgtg ataaagtttt ttaaaaaagg agtgtttatt ttgaataaaa acacagtgtt
     1801 agatgaaggt tacgttatag caactatttt aaatgttttc tttttacttg gattaatttt
     1861 tatatctcat ttggaaaatt tatatatatt aattccgtat gttattttga tgggaataaa
     1921 tgctatttat ttggttgtta aggttatgaa tttcaaaaag aataattaga atatttgagg
     1981 ggattttgaa acgagtttct tcttgttttc acactgtttt tttattccta ttggtgttgt
     2041 tgcttacttt ttgtttttct ataaagatga tgcttttgac gagatggaag aaaaatatga
     2101 ttatcatgaa gataataaaa aatagacgac gcatttatgc cgagaaaatt tattgatgtt
     2161 gagaagaacc cttaactaaa cttgcagacg aatgtcggca tagcgtgagc tattaagccg
     2221 accatgcgac aagttttggg attgttaagg gttccgaggc tcaacgtcaa taaagcaatt
     2281 ggaataaata gtatctaaag taacccgtat ttaagattac attgtacatc cttcgaaacc
     2341 atagtagctg cgattccagc taggtttcct tttatcccaa ttttagctaa tttttttgct
     2401 acgcccttta agtttttggc tttaacatct tcaaaaactg ctgtgattat gtttccagtt
     2461 aaaagttctc catatgagtt taaaacttca gaatagaaac agtcaccgct atttttgtag
     2521 ttcccaccaa ctgctctagg tgatattgaa ctagttttct ttttattctc tatgtcgttt
     2581 tttagttttt ggaatccttc aggaacatat ccatatcgag atttggcatt ttctagatta
     2641 acatctttag ctgttccgtc tttatctaaa ataataattt tgccatatat gtattttagt
     2701 tctttttcaa tttctttttc gtttaaagtt tcaacgtttt tatctgcaat gttatttttg
     2761 ttttgggctt gagcttcttt gaaattaaaa tccaagacca ttgaaagtat ggttaaaata
     2821 cttaaagata ctactaaaat ctttcttagt gatatactgt tttgaattgc attcattgta
     2881 aatgccccct tgttttgtta tttaaatgaa ttcaatataa tattaacata taaaaacaaa
     2941 ggagtttaat atgaataagt ttttttactt tttgattatt ttattatctg tatttttaat
     3001 ttttaatttt gttaattcga ttttatttga taatcctttt gactttttag aaagttttat
     3061 ttattcattg gttttcacat taatttattt aggtttagag ggtcataaaa agaagaagga
     3121 ataggttgtt ttttgaaac
//
