LOCUS       NZ_CP120161             3157 bp    DNA     circular CON 06-JAN-2024
DEFINITION  Mammaliicoccus lentus strain Dog026 plasmid unnamed6, complete
            sequence.
ACCESSION   NZ_CP120161
VERSION     NZ_CP120161.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33696772
            Assembly: GCF_034042815.1
KEYWORDS    RefSeq.
SOURCE      Mammaliicoccus lentus
  ORGANISM  Mammaliicoccus lentus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Mammaliicoccus.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Canine Staphylococcaceae circulating in a Kenyan animal shelter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
  REMARK    Annotation added by database at the request of the submitter
REFERENCE   3  (bases 1 to 3157)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2022
            Assembly Method                   :: Flye v. v2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 283x
            Sequencing Technology             :: PacBio Sequel II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/06/2024 10:51:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,820
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 81
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 7 of 24
            Pseudo Genes (incomplete)         :: 16 of 24
            Pseudo Genes (internal stop)      :: 5 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP120161.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Mammaliicoccus lentus"
                     /mol_type="genomic DNA"
                     /strain="Dog026"
                     /isolation_source="nasal swab"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:42858"
                     /plasmid="unnamed6"
                     /geo_loc_name="Kenya:Nairobi"
                     /lat_lon="1.3345 S 36.7331 E"
                     /collection_date="2015-06-27"
                     /collected_by="Joerg Jores, Anna Lacasta, Anne Liljander,
                     Paul Ssajjakambwe (International Livestock Research
                     Institute, ILRI)"
     gene            complement(278..442)
                     /locus_tag="P3U54_RS14490"
                     /old_locus_tag="P3U54_14495"
     CDS             complement(278..442)
                     /locus_tag="P3U54_RS14490"
                     /old_locus_tag="P3U54_14495"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_323711125.1"
                     /translation="MEYNNTIYFENGIEHTLTEKELVLIKRLLYSAKVENGYEEELENL
                     KNLFLEHLD"
                     /name="hypothetical protein"
     gene            complement(442..1152)
                     /locus_tag="P3U54_RS14495"
                     /old_locus_tag="P3U54_14500"
     CDS             complement(442..1152)
                     /locus_tag="P3U54_RS14495"
                     /old_locus_tag="P3U54_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010892286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="WP_323711126.1"
                     /translation="MTKQITKARNFTFIIYPESIPEDWELYLEKLDVPMAISPLHDKDE
                     TERKFEELTEDEQVIINNGGKVYKKPHYHVLYIAKNPVTTESIRKKIKRALGNKSLSHI
                     EIVDGVESVYKYLTHESKDAIRKNKHKYDSEDIIHLNDFDIERYIFLDESQKRTLKNDL
                     LNIVKTKHIVNVIDLMSFLDMYGEEYGIDNMNYVQDVITSNASAFRLWFEGNYQCGYRA
                     IYSKTINPETGEIK"
                     /name="replication protein"
     gene            complement(1227..1367)
                     /locus_tag="P3U54_RS14500"
                     /old_locus_tag="P3U54_14505"
     CDS             complement(1227..1367)
                     /locus_tag="P3U54_RS14500"
                     /old_locus_tag="P3U54_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009385926.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="WP_323711127.1"
                     /translation="MTEKKRFTITMSDSFYEYLEKLCKEKGLSKSAMLTLILDEYKKGE
                     K"
                     /name="ribbon-helix-helix protein, CopG family"
     gene            complement(1930..3141)
                     /gene="mobV"
                     /locus_tag="P3U54_RS14505"
                     /old_locus_tag="P3U54_14510"
     CDS             complement(1930..3141)
                     /gene="mobV"
                     /locus_tag="P3U54_RS14505"
                     /old_locus_tag="P3U54_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002493196.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_323711124.1"
                     /translation="MSYSIIRVSKVKTGTNTTGIQKHVQRENNNYENEDIDHSKTYLNY
                     DLVNANKQNFNNLIDEKIEQNYTGKRKIRTDAIKHIDGLITSDNDFFDNQTPEDTKQFF
                     EYAKEFLEQEYGKDNLLYATVHMDEKTPHMHYGVVPITDDGRLSAKEVVGNKKALTAFQ
                     DRFNEYVNQRGYDLDRGQSRQVTNAKHDQVSRYKQKTEYHKQEYERESQKLSHIQQKSS
                     ELIVQYQKSLETLKKPLNVQYEHETEKVGGLFNKEIQETGNVVISQEEFNNFQKQIQAA
                     QTITEDYEYIKSDKALNDLKNENSKLREENKDMSETLARANEFIKDLEKHLKKALNVIK
                     VIREIFEKLEQAIGRNKYRNLMNDVSRDNAPMIKAIQMFDKQIHPEEYQEEKNTQKRDR
                     GRGR"
                     /name="mobV"
     Typing          complement(1934..3141)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.27"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tatataattc actcctcaac aataaggtta gttaggtaat ttaatacttt aagtctaaca
       61 cactagactt attttgcaat aagtcgttaa tacgtgtgct ctgcgaggct taaacctgtt
      121 tttgtgcaag cacaaaaaat ggactgaaca agtcagtcca taagttcaaa acctaaacac
      181 cagcaaccaa aaatggtggt tgctcatgtt cataaatttt atgatcacta tcgtgaacta
      241 aaatttaatt cactaagttc aaattcaaaa tctaaaatca atccaaatgt tctaaaaata
      301 aattcttcaa gttttctaat tcttcttcat aaccattttc aactttagca gaatatagca
      361 atcgtttaat taaaaccaat tctttttctg ttaatgtatg ttcaattcca ttttcaaaat
      421 atattgtatt attatactcc attactttat ctcccctgtt tccggattaa ttgtttttga
      481 atatatggct ctatatccac attgatagtt accttcaaac caaagtctaa aagcactagc
      541 attggacgta ataacatctt gaacataatt catattatca atgccatatt cttctccata
      601 catatccaga aatgacatta aatcaattac attaacgata tgtttagttt taactatatt
      661 taataaatca ttttttaaag tacgcttttg actttcatct aaaaaaatat atctctcaat
      721 atcaaaatca tttaaatgaa taatatcttc actatcatat ttatgtttgt tttttctaat
      781 agcatcttta ctttcgtgtg ttaaatattt ataaacactc tcgacaccat ctacaatttc
      841 tatatgagat aaagatttat ttcctaacgc tcgttttatt ttctttctta tactttcagt
      901 tgtaacagga tttttagcaa tatataaaac gtgatagtgt ggctttttat agaccttacc
      961 accgttattt ataattactt gttcatcttc ggttaactct tcaaactttc tttctgtttc
     1021 atctttatca tgtaacggac taatcgccat tggaacatca agtttttcta aatataattc
     1081 ccaatcttct ggtatacttt ctggataaat aataaacgta aagtttctag cctttgttat
     1141 ttgtttagtc atgttatata attacctttg aaaatataag catatattca agagcggtct
     1201 tttgcgagga cgctcttttt ttatactcat ttttctcctt tcttatattc atctaaaatc
     1261 aatgtaagca ttgctgattt actcaatccc ttttctttac ataacttttc taaatactca
     1321 taaaaactat ccgacatagt aattgtaaat cttttctttt cagtcatttt ttctcctctc
     1381 aatcaagtca atttattaaa aacatagtat catagttgtg tactttatac aaataaatac
     1441 gtaaaaatgg tgaaacgttg gaaaaatcac caacaaacgg cggtcatttt aaaatgcttg
     1501 tatcccttgt gccagtaggt ttaaaccatg attattgcga agtgttggca ctgggctcaa
     1561 taatgggggg gtcgtagtag ccccccattt tccaactaaa tttgataaat tctaaaccaa
     1621 aatacaaaaa cagggctaac agatcacttc gtgaagttag ccctaaaacc taaaccatta
     1681 actcaattta tttttttatt ccaatttcct tttggtctta aagtttgcgt ccttaacaat
     1741 cctaaaagct gtcagtcggt cgggttaata gctgacgcta tcgaccgctg tcgctttaag
     1801 tctaagttaa ggactcttta agacaaaaag ttttctcggc ataaacgtgg gtataattaa
     1861 attagatata cttaaaacaa ttcttttaag attttgaacg ccattctatt tttagagtgg
     1921 cgtttttatt tatctacctc tacccctatc acgtttttgc gtattctttt cttcttgata
     1981 ttcttcaggg tgtatttgtt tatcaaacat ttgtattgcc tttatcatag gagcattatc
     2041 tctactcaca tcattcatta aattcctata tttatttcta ccaatagctt gctctaactt
     2101 ctcaaaaatc tctctaataa ctttaataac attaagcgcc tttttaaggt gtttttcgag
     2161 gtcttttata aactcatttg ctcttgctaa agtttcagac atatccttgt tttcttctct
     2221 aagcttacta ttttcgtttt ttaggtcatt cagagcttta tctgacttaa tatactcata
     2281 atcttctgta atcgtttgtg ctgcttgtat ttgtttctga aagttattaa attcttcttg
     2341 gcttattaca acattccctg tttcttgtat ttctttgttg aacaatccac ctactttttc
     2401 agtttcatgt tcatattgga cgtttaaagg ctttttaagc gtttctaatg atttttggta
     2461 ttgtactatc aactcactgc ttttttgttg tatatggctt aatttttggc tctcacgctc
     2521 atattcttgc ttatggtatt ctgttttttg cttatatcgg cttacctgat catgtttagc
     2581 atttgtaact tgtcttgatt gtcctcggtc caaatcatac ccacgttgat taacatattc
     2641 gttaaatcta tcttgaaacg ctgttaacgc ctttttattc cctacaactt cttttgcact
     2701 taatctaccg tcatcagtga tagggacaac accataatgc atatgtgggg ttttttcgtc
     2761 catatgaact gttgcatata ataaattatc tttaccgtat tcttgttcta aaaactcttt
     2821 agcatattca aaaaactgct ttgtatcttc tggcgtttga ttatcaaaaa aatcattgtc
     2881 tgatgtaatt aaaccgtcaa tgtgtttaat cgcgtctgtt ctaatttttc gtttgcctgt
     2941 ataattctgt tcaatttttt catcaatcaa gttattaaaa ttctgtttat tagcatttac
     3001 caaatcataa tttaagtaag ttttactatg gtctatatct tcattttcat aattattatt
     3061 ttctctttga acatgttttt gtatgcctgt tgtatttgtt cctgttttga cttttgaaac
     3121 tctaataatt gaatacgaca tatataattc actcctc
//
