LOCUS       NZ_CP120154             3149 bp    DNA     circular CON 06-JAN-2024
DEFINITION  Staphylococcus aureus strain Dog027 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP120154
VERSION     NZ_CP120154.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33696773
            Assembly: GCF_034042795.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Canine Staphylococcaceae circulating in a Kenyan animal shelter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
  REMARK    Annotation added by database at the request of the submitter
REFERENCE   3  (bases 1 to 3149)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2022
            Assembly Method                   :: Flye v. v2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 381x
            Sequencing Technology             :: PacBio Sequel II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/06/2024 10:55:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,833
            CDSs (total)                      :: 2,751
            Genes (coding)                    :: 2,664
            CDSs (with protein)               :: 2,664
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 46 of 87
            Pseudo Genes (incomplete)         :: 35 of 87
            Pseudo Genes (internal stop)      :: 21 of 87
            Pseudo Genes (multiple problems)  :: 15 of 87
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP120154.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="Dog027"
                     /isolation_source="nasal swab"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed2"
                     /geo_loc_name="Kenya:Nairobi"
                     /lat_lon="1.3345 S 36.7331 E"
                     /collection_date="2015-06-27"
                     /collected_by="Joerg Jores, Anna Lacasta, Anne Liljander,
                     Paul Ssajjakambwe (International Livestock Research
                     Institute, ILRI)"
     gene            join(3000..3149,1..27)
                     /locus_tag="P3T91_RS14155"
                     /old_locus_tag="P3T91_14155"
     CDS             join(3000..3149,1..27)
                     /locus_tag="P3T91_RS14155"
                     /old_locus_tag="P3T91_14155"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_323710431.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIAD
                     KNVETLQIKTLKL"
                     /name="hypothetical protein"
     gene            complement(781..969)
                     /locus_tag="P3T91_RS14160"
                     /old_locus_tag="P3T91_14160"
     CDS             complement(781..969)
                     /locus_tag="P3T91_RS14160"
                     /old_locus_tag="P3T91_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001039248.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     gene            complement(1553..2548)
                     /locus_tag="P3T91_RS14165"
                     /old_locus_tag="P3T91_14165"
     CDS             complement(1553..2548)
                     /locus_tag="P3T91_RS14165"
                     /old_locus_tag="P3T91_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001199892.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     Typing          complement(1319..1483)
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.42e-81"
     Typing          complement(1554..2548)
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.9"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgcagataa aaacgttgaa actttaaacg aaaaagaaat tgaaaaagaa ctaaaataca
       61 tatatggcaa aattattatt ttagataaag acggaacagc taaagatgtt aatctagaaa
      121 atgccaaatc tcgatatgga tatgttcctg aaggattcca aaaactaaaa aacgacatag
      181 agaataaaaa gaaaactagt tcaatatcac ctagagcagt tggtgggaac tacaaaaata
      241 gcggtgactg tttctattct gaagttttaa actcatatgg agaactttta actggaaaca
      301 taatcacagc agtttttgaa gatgttaaag ccaaaaactt aaagggcgta gcaaaaaaat
      361 tagctaaaat tgggataaaa ggaaacctag ctggaatcgc agctactatg gtttcgaagg
      421 atgtacaatg taatcttaaa tacgggttac tttagatact atttattcca attgctttat
      481 tgacgttgag cctcggaacc cttaacaatc ccaaaacttg tcgcatggtc ggcttaatag
      541 ctcacgctat gccgacattc gtctgcaagt ttagttaagg gttcttctca acatcaataa
      601 attttctcgg cataaatgcg tcgtctattt tttattatct tcatgataat catatttttc
      661 ttccatctcg tcaaaagcat catctttata gaaaaacaaa aagtaagcaa caacaccaat
      721 aggaataaaa aaacagtgtg aaaacaagaa gaaactcgtt tcaaaatccc ctcaaatatt
      781 ctaattattc tttttgaaat tcataacctt aacaaccaaa taaatagcat ttattcccat
      841 caaaataaca tacggaatta atatatataa attttccaaa tgagatataa aaattaatcc
      901 aagtaaaaag aaaacattta aaatagttgc tataacgtaa ccttcatcta acactgtgtt
      961 tttattcaaa ataaacactc cttttttaaa aaactttatc acaagactta ctcaaattat
     1021 aaataccttt attttgagtt ttaagggggt attttaaagg tttagggggg tatcatatag
     1081 gtttttaact taaaaatata tacaagctct taaaacgcaa atatgagcca aataaatata
     1141 tggtaatttc acaaacaaaa atttcagcaa aaccagtgac taatttttta acactgccca
     1201 tttacatgca aattaaaaat tagcataaaa tcctttaaaa cttccacatg tttttctaaa
     1261 ttcctgcagg gcgtttttta tcaaaaatcg agaattttta atttttctct aaaaactccg
     1321 taagagcata aggagaattt gctatcattc aaaaatgata ggtctggcaa agccagacat
     1381 tccaggggtt ttaaggtatt ttaaagtgat atcatacaaa gctgtactgt cttatttttg
     1441 tgacacaata tattgcgttt gtcttatttt tgtgacacta catacagcat ttgtcacaaa
     1501 aataagacag tactttttta taaaaaatca acaaaaaaga cattagtcaa cactaatgtc
     1561 ttaaataata attttgcttt tcgaaattcc aaattgcggt aattgaatgt gctttttctt
     1621 cttcgtctgt tgttttatct tcatcacttg cattaatcaa attaccatct tcgacatcgt
     1681 ctaagtttaa aattttatgt ttttgtttaa gcaatccacc ataacttaac atacgttttc
     1741 gatacaaacc tttttctaaa tcacttacaa tttcttgatt tttttcatca tcatcagtta
     1801 aaaaatcaga tgatttaacc gaatattttg aggtctcttt gattgccgat tcaatgtctt
     1861 tatcgccttt tctattcggt ttgatcgctt taacattagc aacaggtcga tagtcaactt
     1921 gtaatgctct ttgccataaa ttaacccatt cttcttgagt tatataattc tctttttttc
     1981 taaaatatgc attttcaaca cataacaaaa catgcatgtg ctgattataa ctaccgtcat
     2041 ttttattaac ggtaacttct gttgaacgca taaatccaac aagattttgt ttaacctttt
     2101 tatatctact caacctatca aatgctttag ttagatgctt caaactttgt tctaaagtat
     2161 ctccatctat cgcatttttt gttgaaagtg ttaaaaacaa ccaacgtgct tttggctttt
     2221 ccttaattac tttttcaatc actttttgag cttgataact atttttcata gcacgtctcc
     2281 aattacaaac cggacataat tttgatttac aaaaccatgt cttatgtaac ttcaaatagc
     2341 cttcatcagt tggcttaaat tctaaaatat taccacattg ttttacatta aaagcctttt
     2401 tgatttttaa aatttctagt atatctgcat agcttacatt atctattttc ttttctctcc
     2461 atgggcgttg tttcccactt ttcgtcatat cttttaacgt tttattatct tgattttcgg
     2521 ttatacttct agtagtatta tattgcatat aaaaagaccc ccagtattat ttaagattcg
     2581 acaccttaat aatataactg gggtttttta ttgtcaaatt ttcctttcac gcacaaaaac
     2641 acgtttaaac actgtaatat caacagttac agctatatat ataaaatcga gttatttcta
     2701 tatagtatca agacaagaag aaactcgttc acactcgttt caaaaaacaa cctattcctt
     2761 cttcttttta tgaccctcta aacctaaata aattaatgtg aaaaccaatg aataaataaa
     2821 actttctaaa aagtcaaaag gattatcaaa taaaatcgaa ttaacaaaat taaaaattaa
     2881 aaatacagat aataaaataa tcaaaaagta aaaaaactta ttcatattaa actcctttgt
     2941 ttttatatgt taatattata ttgaattcat ttaaataaca aaacaagggg gcatttacaa
     3001 tgaatgcaat tcaaaacagt atatcactaa gaaagatttt agtagtatct ttaagtattt
     3061 taaccatact ttcaatggtc ttggatttta atttcaaaga agctcaagcc caaaacaaaa
     3121 ataacattgc agataaaaac gttgaaact
//
