LOCUS       NZ_CP120145             3128 bp    DNA     circular CON 06-JAN-2024
DEFINITION  Staphylococcus aureus strain Dog032 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP120145
VERSION     NZ_CP120145.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33696778
            Assembly: GCF_034042695.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Canine Staphylococcaceae circulating in a Kenyan animal shelter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
  REMARK    Annotation added by database at submitter's request
REFERENCE   3  (bases 1 to 3128)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2022
            Assembly Method                   :: Flye v. v2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 454x
            Sequencing Technology             :: PacBio Sequel II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/06/2024 11:03:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,759
            CDSs (total)                      :: 2,677
            Genes (coding)                    :: 2,596
            CDSs (with protein)               :: 2,596
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 43 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 19 of 81
            Pseudo Genes (multiple problems)  :: 15 of 81
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP120145.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="Dog032"
                     /isolation_source="nasal swab"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed2"
                     /geo_loc_name="Kenya:Nairobi"
                     /lat_lon="1.3345 S 36.7331 E"
                     /collection_date="2015-06-27"
                     /collected_by="Joerg Jores, Anna Lacasta, Anne Liljander,
                     Paul Ssajjakambwe (International Livestock Research
                     Institute, ILRI)"
     gene            complement(203..1198)
                     /locus_tag="P3U30_RS13785"
                     /old_locus_tag="P3U30_13785"
     CDS             complement(203..1198)
                     /locus_tag="P3U30_RS13785"
                     /old_locus_tag="P3U30_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001199892.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     gene            1650..2231
                     /locus_tag="P3U30_RS13790"
                     /old_locus_tag="P3U30_13790"
     CDS             1650..2231
                     /locus_tag="P3U30_RS13790"
                     /old_locus_tag="P3U30_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000991705.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIAD
                     KNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDIEN
                     KKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVAKKL
                     AKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
                     /name="hypothetical protein"
     gene            complement(2557..2745)
                     /locus_tag="P3U30_RS13795"
                     /old_locus_tag="P3U30_13795"
     CDS             complement(2557..2745)
                     /locus_tag="P3U30_RS13795"
                     /old_locus_tag="P3U30_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001039248.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     Typing          complement(2..133)
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.35e-63"
     Typing          complement(204..1198)
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.9"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaaatgata ggtctggcaa agccagacat tccaggggtt ttaaggtatt ttaaagtgat
       61 atcatacaaa gctgtactgt cttatttttg tgacacaata tattgcgttt gtcttatttt
      121 tgtgacacta catacagcat ttgtcacaaa aataagacag tactttttta taaaaaatca
      181 acaaaaaaga cattagtcaa cactaatgtc ttaaataata attttgcttt tcgaaattcc
      241 aaattgcggt aattgaatgt gctttttctt cttcgtctgt tgttttatct tcatcacttg
      301 cattaatcaa attaccatct tcgacatcgt ctaagtttaa aattttatgt ttttgtttaa
      361 gcaatccacc ataacttaac atacgttttc gatacaaacc tttttctaaa tcacttacaa
      421 tttcttgatt tttttcatca tcatcagtta aaaaatcaga tgatttaacc gaatattttg
      481 aggtctcttt gattgccgat tcaatgtctt tatcgccttt tctattcggt ttgatcgctt
      541 taacattagc aacaggtcga tagtcaactt gtaatgctct ttgccataaa ttaacccatt
      601 cttcttgagt tatataattc tctttttttc taaaatatgc attttcaaca cataacaaaa
      661 catgcatgtg ctgattataa ctaccgtcat ttttattaac ggtaacttct gttgaacgca
      721 taaatccaac aagattttgt ttaacctttt tatatctact caacctatca aatgctttag
      781 ttagatgctt caaactttgt tctaaagtat ctccatctat cgcatttttt gttgaaagtg
      841 ttaaaaacaa ccaacgtgct tttggctttt ccttaattac tttttcaatc actttttgag
      901 cttgataact atttttcata gcacgtctcc aattacaaac cggacataat tttgatttac
      961 aaaaccatgt cttatgtaac ttcaaatagc cttcatcagt tggcttaaat tctaaaatat
     1021 taccacattg ttttacatta aaagcctttt tgatttttaa aatttctagt atatctgcat
     1081 agcttacatt atctattttc ttttctctcc atgggcgttg tttcccactt ttcgtcatat
     1141 cttttaacgt tttattatct tgattttcgg ttatacttct agtagtatta tattgcatat
     1201 aaaaagaccc ccagtattat ttaagattcg acaccttaat aatataactg gggtttttta
     1261 ttgtcaaatt ttcctttcac gcacaaaaac acgtttaaac actgtaatat caacagttac
     1321 agctatatat ataaaatcga gttatttcta tatagtatca agacaagaag aaactcgttc
     1381 acactcgttt caaaaaacaa cctattcctt cttcttttta tgaccctcta aacctaaata
     1441 aattaatgtg aaaaccaatg aataaataaa actttctaaa aagtcaaaag gattatcaaa
     1501 taaaatcgaa ttaacaaaat taaaaattaa aaatacagat aataaaataa tcaaaaagta
     1561 aaaaaactta ttcatattaa actcctttgt ttttatatgt taatattata ttgaattcat
     1621 ttaaataaca aaacaagggg gcatttacaa tgaatgcaat tcaaaacagt atatcactaa
     1681 gaaagatttt agtagtatct ttaagtattt taaccatact ttcaatggtc ttggatttta
     1741 atttcaaaga agctcaagcc caaaacaaaa ataacattgc agataaaaac gttgaaactt
     1801 taaacgaaaa agaaattgaa aaagaactaa aatacatata tggcaaaatt attattttag
     1861 ataaagacgg aacagctaaa gatgttaatc tagaaaatgc caaatctcga tatggatatg
     1921 ttcctgaagg attccaaaaa ctaaaaaacg acatagagaa taaaaagaaa actagttcaa
     1981 tatcacctag agcagttggt gggaactaca aaaatagcgg tgactgtttc tattctgaag
     2041 ttttaaactc atatggagaa cttttaactg gaaacataat cacagcagtt tttgaagatg
     2101 ttaaagccaa aaacttaaag ggcgtagcaa aaaaattagc taaaattggg ataaaaggaa
     2161 acctagctgg aatcgcagct actatggttt cgaaggatgt acaatgtaat cttaaatacg
     2221 ggttacttta gatactattt attccaattg ctttattgac gttgagcctc ggaaccctta
     2281 acaatcccaa aacttgtcgc atggtcggct taatagctca cgctatgccg acattcgtct
     2341 gcaagtttag ttaagggttc ttctcaacat caataaattt tctcggcata aatgcgtcgt
     2401 ctatttttta ttatcttcat gataatcata tttttcttcc atctcgtcaa aagcatcatc
     2461 tttatagaaa aacaaaaagt aagcaacaac accaatagga ataaaaaaac agtgtgaaaa
     2521 caagaagaaa ctcgtttcaa aatcccctca aatattctaa ttattctttt tgaaattcat
     2581 aaccttaaca accaaataaa tagcatttat tcccatcaaa ataacatacg gaattaatat
     2641 atataaattt tccaaatgag atataaaaat taatccaagt aaaaagaaaa catttaaaat
     2701 agttgctata acgtaacctt catctaacac tgtgttttta ttcaaaataa acactccttt
     2761 tttaaaaaac tttatcacaa gacttactca aattataaat acctttattt tgagttttaa
     2821 gggggtattt taaaggttta ggggggtatc atataggttt ttaacttaaa aatatataca
     2881 agctcttaaa acgcaaatat gagccaaata aatatatggt aatttcacaa acaaaaattt
     2941 cagcaaaacc agtgactaat tttttaacac tgcccattta catgcaaatt aaaaattagc
     3001 ataaaatcct ttaaaacttc cacatgtttt tctaaattcc tgcagggcgt tttttatcaa
     3061 aaatcgagaa tttttaattt ttctctaaaa actccgtaag agcataagga gaatttgcta
     3121 tcattcaa
//
