LOCUS       NZ_CP120136             3135 bp    DNA     circular CON 06-JAN-2024
DEFINITION  Staphylococcus aureus strain Dog043 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP120136
VERSION     NZ_CP120136.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33696784
            Assembly: GCF_034041215.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Canine Staphylococcaceae circulating in a Kenyan animal shelter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
  REMARK    Annotation added by database at submitter's request
REFERENCE   3  (bases 1 to 3135)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2022
            Assembly Method                   :: Flye v. v2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 574x
            Sequencing Technology             :: PacBio Sequel II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/06/2024 10:59:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,823
            CDSs (total)                      :: 2,741
            Genes (coding)                    :: 2,653
            CDSs (with protein)               :: 2,653
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 34 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP120136.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="Dog043"
                     /isolation_source="nasal swab"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed2"
                     /geo_loc_name="Kenya:Nairobi"
                     /lat_lon="1.3345 S 36.7331 E"
                     /collection_date="2015-06-27"
                     /collected_by="Joerg Jores, Anna Lacasta, Anne Liljander,
                     Paul Ssajjakambwe (International Livestock Research
                     Institute, ILRI)"
     gene            join(2970..3135,1..425)
                     /locus_tag="P3U51_RS14105"
                     /old_locus_tag="P3U51_14105"
                     /pseudo=""
     CDS             join(2970..3135,1..425)
                     /locus_tag="P3U51_RS14105"
                     /old_locus_tag="P3U51_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(751..939)
                     /locus_tag="P3U51_RS14110"
                     /old_locus_tag="P3U51_14110"
     CDS             complement(751..939)
                     /locus_tag="P3U51_RS14110"
                     /old_locus_tag="P3U51_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001039248.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     gene            complement(1523..2518)
                     /locus_tag="P3U51_RS14115"
                     /old_locus_tag="P3U51_14115"
     CDS             complement(1523..2518)
                     /locus_tag="P3U51_RS14115"
                     /old_locus_tag="P3U51_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001199892.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     Typing          complement(1289..1453)
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.42e-81"
     Typing          complement(1524..2518)
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.9"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaaagaaat tgaaaaagaa ctaaaataca tatatggcaa aattattatt ttagataaag
       61 acggaacagc taaagatgtt aatctagaaa atgccaaatc tcgatatgga tatgttcctg
      121 aaggattcca aaaactaaaa aacgacatag agaataaaaa gaaaactagt tcaatatcac
      181 ctagagcagt tggtgggaac tacaaaaata gcggtgactg tttctattct gaagttttaa
      241 actcatatgg agaactttta actggaaaca taatcacagc agtttttgaa gatgttaaag
      301 ccaaaaactt aaagggcgta gcaaaaaaat tagctaaaat tgggataaaa ggaaacctag
      361 ctggaatcgc agctactatg gtttcgaagg atgtacaatg taatcttaaa tacgggttac
      421 tttagatact atttattcca attgctttat tgacgttgag cctcggaacc cttaacaatc
      481 ccaaaacttg tcgcatggtc ggcttaatag ctcacgctat gccgacattc gtctgcaagt
      541 ttagttaagg gttcttctca acatcaataa attttctcgg cataaatgcg tcgtctattt
      601 tttattatct tcatgataat catatttttc ttccatctcg tcaaaagcat catctttata
      661 gaaaaacaaa aagtaagcaa caacaccaat aggaataaaa aaacagtgtg aaaacaagaa
      721 gaaactcgtt tcaaaatccc ctcaaatatt ctaattattc tttttgaaat tcataacctt
      781 aacaaccaaa taaatagcat ttattcccat caaaataaca tacggaatta atatatataa
      841 attttccaaa tgagatataa aaattaatcc aagtaaaaag aaaacattta aaatagttgc
      901 tataacgtaa ccttcatcta acactgtgtt tttattcaaa ataaacactc cttttttaaa
      961 aaactttatc acaagactta ctcaaattat aaataccttt attttgagtt ttaagggggt
     1021 attttaaagg tttagggggg tatcatatag gtttttaact taaaaatata tacaagctct
     1081 taaaacgcaa atatgagcca aataaatata tggtaatttc acaaacaaaa atttcagcaa
     1141 aaccagtgac taatttttta acactgccca tttacatgca aattaaaaat tagcataaaa
     1201 tcctttaaaa cttccacatg tttttctaaa ttcctgcagg gcgtttttta tcaaaaatcg
     1261 agaattttta atttttctct aaaaactccg taagagcata aggagaattt gctatcattc
     1321 aaaaatgata ggtctggcaa agccagacat tccaggggtt ttaaggtatt ttaaagtgat
     1381 atcatacaaa gctgtactgt cttatttttg tgacacaata tattgcgttt gtcttatttt
     1441 tgtgacacta catacagcat ttgtcacaaa aataagacag tactttttta taaaaaatca
     1501 acaaaaaaga cattagtcaa cactaatgtc ttaaataata attttgcttt tcgaaattcc
     1561 aaattgcggt aattgaatgt gctttttctt cttcgtctgt tgttttatct tcatcacttg
     1621 cattaatcaa attaccatct tcgacatcgt ctaagtttaa aattttatgt ttttgtttaa
     1681 gcaatccacc ataacttaac atacgttttc gatacaaacc tttttctaaa tcacttacaa
     1741 tttcttgatt tttttcatca tcatcagtta aaaaatcaga tgatttaacc gaatattttg
     1801 aggtctcttt gattgccgat tcaatgtctt tatcgccttt tctattcggt ttgatcgctt
     1861 taacattagc aacaggtcga tagtcaactt gtaatgctct ttgccataaa ttaacccatt
     1921 cttcttgagt tatataattc tctttttttc taaaatatgc attttcaaca cataacaaaa
     1981 catgcatgtg ctgattataa ctaccgtcat ttttattaac ggtaacttct gttgaacgca
     2041 taaatccaac aagattttgt ttaacctttt tatatctact caacctatca aatgctttag
     2101 ttagatgctt caaactttgt tctaaagtat ctccatctat cgcatttttt gttgaaagtg
     2161 ttaaaaacaa ccaacgtgct tttggctttt ccttaattac tttttcaatc actttttgag
     2221 cttgataact atttttcata gcacgtctcc aattacaaac cggacataat tttgatttac
     2281 aaaaccatgt cttatgtaac ttcaaatagc cttcatcagt tggcttaaat tctaaaatat
     2341 taccacattg ttttacatta aaagcctttt tgatttttaa aatttctagt atatctgcat
     2401 agcttacatt atctattttc ttttctctcc atgggcgttg tttcccactt ttcgtcatat
     2461 cttttaacgt tttattatct tgattttcgg ttatacttct agtagtatta tattgcatat
     2521 aaaaagaccc ccagtattat ttaagattcg acaccttaat aatataactg gggtttttta
     2581 ttgtcaaatt ttcctttcac gcacaaaaac acgtttaaac actgtaatat caacagttac
     2641 agctatatat ataaaatcga gttatttcta tatagtatca agacaagaag aaactcgttc
     2701 acactcgttt caaaaaacaa cctattcctt cttcttttta tgaccctcta aacctaaata
     2761 aattaatgtg aaaaccaatg aataaataaa actttctaaa aagtcaaaag gattatcaaa
     2821 taaaatcgaa ttaacaaaat taaaaattaa aaatacagat aataaaataa tcaaaaagta
     2881 aaaaaactta ttcatattaa actcctttgt ttttatatgt taatattata ttgaattcat
     2941 ttaaataaca aaacaagggg gcatttacaa tgaatgcaat tcaaaacagt atatcactaa
     3001 gaaagatttt agtagtatct ttaagtattt taaccatact ttcaatggtc ttggatttta
     3061 atttcaaaga agctcaagcc caaaacaaaa ataacattgc agataaaaac gttgaaactt
     3121 taaacgaaaa agaaa
//
