LOCUS       NZ_CP120127             3090 bp    DNA     circular CON 06-JAN-2024
DEFINITION  Staphylococcus aureus strain Dog052 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP120127
VERSION     NZ_CP120127.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33696790
            Assembly: GCF_034035835.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Canine Staphylococcaceae circulating in a Kenyan animal shelter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
  REMARK    Annotation added by database at submitter's request
REFERENCE   3  (bases 1 to 3090)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2022
            Assembly Method                   :: Flye v. v2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 309x
            Sequencing Technology             :: PacBio Sequel II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/06/2024 10:54:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,832
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,663
            CDSs (with protein)               :: 2,663
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 46 of 87
            Pseudo Genes (incomplete)         :: 35 of 87
            Pseudo Genes (internal stop)      :: 21 of 87
            Pseudo Genes (multiple problems)  :: 15 of 87
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP120127.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="Dog052"
                     /isolation_source="nasal swab"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed2"
                     /geo_loc_name="Kenya:Nairobi"
                     /lat_lon="1.3345 S 36.7331 E"
                     /collection_date="2015-06-27"
                     /collected_by="Joerg Jores, Anna Lacasta, Anne Liljander,
                     Paul Ssajjakambwe (International Livestock Research
                     Institute, ILRI)"
     gene            407..595
                     /locus_tag="P3U11_RS14150"
                     /old_locus_tag="P3U11_14150"
     CDS             407..595
                     /locus_tag="P3U11_RS14150"
                     /old_locus_tag="P3U11_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001039248.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     gene            complement(921..1502)
                     /locus_tag="P3U11_RS14155"
                     /old_locus_tag="P3U11_14155"
     CDS             complement(921..1502)
                     /locus_tag="P3U11_RS14155"
                     /old_locus_tag="P3U11_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000991705.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIAD
                     KNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDIEN
                     KKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVAKKL
                     AKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
                     /name="hypothetical protein"
     gene            1954..2913
                     /locus_tag="P3U11_RS14160"
                     /old_locus_tag="P3U11_14160"
     CDS             1954..2913
                     /locus_tag="P3U11_RS14160"
                     /old_locus_tag="P3U11_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_323711130.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQVDYRPVANVKAIKPNRKGDKDIES
                     AIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLKQKHKILNLDDVE
                     DGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     Typing          2984..3090
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.22e-49"
     Typing          1955..2548
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.832"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          2546..2913
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 tggctttgcc agacctatca tttttgaatg atagcaaatt ctccttatgc tcttacggag
       61 tttttagaga aaaattaaaa attctcgatt tttgataaaa aacgccctgc aggaatttag
      121 aaaaacatgt ggaagtttta aaggatttta tgctaatttt taatttgcat gtaaatgggc
      181 agtgttaaaa aattagtcac tggttttgct gaaatttttg tttgtgaaat taccatatat
      241 ttatttggct catatttgcg ttttaagagc ttgtatatat ttttaagtta aaaacctata
      301 tgataccccc ctaaaccttt aaaatacccc cttaaaactc aaaataaagg tatttataat
      361 ttgagtaagt cttgtgataa agttttttaa aaaaggagtg tttattttga ataaaaacac
      421 agtgttagat gaaggttacg ttatagcaac tattttaaat gttttctttt tacttggatt
      481 aatttttata tctcatttgg aaaatttata tatattaatt ccgtatgtta ttttgatggg
      541 aataaatgct atttatttgg ttgttaaggt tatgaatttc aaaaagaata attagaatat
      601 ttgaggggat tttgaaacga gtttcttctt gttttcacac tgttttttta ttcctattgg
      661 tgttgttgct tactttttgt ttttctataa agatgatgct tttgacgaga tggaagaaaa
      721 atatgattat catgaagata ataaaaaata gacgacgcat ttatgccgag aaaatttatt
      781 gatgttgaga agaaccctta actaaacttg cagacgaatg tcggcatagc gtgagctatt
      841 aagccgacca tgcgacaagt tttgggattg ttaagggttc cgaggctcaa cgtcaataaa
      901 gcaattggaa taaatagtat ctaaagtaac ccgtatttaa gattacattg tacatccttc
      961 gaaaccatag tagctgcgat tccagctagg tttcctttta tcccaatttt agctaatttt
     1021 tttgctacgc cctttaagtt tttggcttta acatcttcaa aaactgctgt gattatgttt
     1081 ccagttaaaa gttctccata tgagtttaaa acttcagaat agaaacagtc accgctattt
     1141 ttgtagttcc caccaactgc tctaggtgat attgaactag ttttcttttt attctctatg
     1201 tcgtttttta gtttttggaa tccttcagga acatatccat atcgagattt ggcattttct
     1261 agattaacat ctttagctgt tccgtcttta tctaaaataa taattttgcc atatatgtat
     1321 tttagttctt tttcaatttc tttttcgttt aaagtttcaa cgtttttatc tgcaatgtta
     1381 tttttgtttt gggcttgagc ttctttgaaa ttaaaatcca agaccattga aagtatggtt
     1441 aaaatactta aagatactac taaaatcttt cttagtgata tactgttttg aattgcattc
     1501 attgtaaatg cccccttgtt ttgttattta aatgaattca atataatatt aacatataaa
     1561 aacaaaggag tttaatatga ataagttttt ttactttttg attattttat tatctgtatt
     1621 tttaattttt aattttgtta attcgatttt atttgataat ccttttgact ttttagaaag
     1681 ttttatttat tcattggttt tcacattaat ttatttaggt ttagagggtc ataaaaagaa
     1741 gaaggaatag gttgtttttt gaaacgagtg tgaacgagtt tcttcttgtc ttgatactat
     1801 atagaaataa ctcgatttta tatatatagc tgtaactgtt gatattacag tgtttaaacg
     1861 tgtttttgtg cgtgaaagga aaatttgaca ataaaaaacc ccagttatat tattaaggtg
     1921 tcgaatctta aataatactg ggggtctttt tatatgcaat ataatactac tagaagtata
     1981 accgaaaatc aagataataa aacgttaaaa gatatgacga aaagtgggaa acaacgccca
     2041 tggagagaaa agaaaataga taatgtaagc tatgcagata tactagaaat tttaaaaatc
     2101 aaaaaggctt ttaatgtaaa acaatgtggt aatattttag aatttaagcc aactgatgaa
     2161 ggctatttga agttacataa gacatggttt tgtaaatcaa aattatgtcc ggtttgtaat
     2221 tggagacgtg ctatgaaaaa tagttatcaa gctcaaaaag tgattgaaaa agtaattaag
     2281 gaaaagccaa aagcacgttg gttgttttta acactttcaa caaaaaatgc gatagatgga
     2341 gatactttag aacaaagttt gaagcatcta actaaagcat ttgataggtt gagtagatat
     2401 aaaaaggtta aacaaaatct tgttggattt atgcgttcaa cagaagttac cgttaataaa
     2461 aatgacggta gttataatca gcacatgcat gttttgttat gtgttgaaaa tgcatatttt
     2521 agaaaaaaag agaattatat aactcaagtt gactatcgac ctgttgctaa tgttaaagcg
     2581 atcaaaccga atagaaaagg cgataaagac attgaatcgg caatcaaaga gacctcaaaa
     2641 tattcggtta aatcatctga ttttttaact gatgatgatg aaaaaaatca agaaattgta
     2701 agtgatttag aaaaaggttt gtatcgaaaa cgtatgttaa gttatggtgg attgcttaaa
     2761 caaaaacata aaattttaaa cttagacgat gtcgaagatg gtaatttgat taatgcaagt
     2821 gatgaagata aaacaacaga cgaagaagaa aaagcacatt caattaccgc aatttggaat
     2881 ttcgaaaagc aaaattatta tttaagacat tagtgttgac taatgtcttt tttgttgatt
     2941 ttttataaaa aagtactgtc ttatttttgt gacaaatgct gtatgtagtg tcacaaaaat
     3001 aagacaaacg caatatattg tgtcacaaaa ataagacagt acagctttgt atgatatcac
     3061 tttaaaatac cttaaaaccc ctggaatgtc
//
