LOCUS       NZ_CP120120             3174 bp    DNA     circular CON 06-JAN-2024
DEFINITION  Staphylococcus aureus strain Dog100 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP120120
VERSION     NZ_CP120120.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33696792
            Assembly: GCF_034035055.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Canine Staphylococcaceae circulating in a Kenyan animal shelter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
  REMARK    Annotation added by database at submitter's request
REFERENCE   3  (bases 1 to 3174)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2022
            Assembly Method                   :: Flye v. v2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 262x
            Sequencing Technology             :: PacBio Sequel II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/06/2024 10:53:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,752
            Genes (coding)                    :: 2,664
            CDSs (with protein)               :: 2,664
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 35 of 88
            Pseudo Genes (internal stop)      :: 21 of 88
            Pseudo Genes (multiple problems)  :: 15 of 88
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP120120.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="Dog100"
                     /isolation_source="nasal swab"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed2"
                     /geo_loc_name="Kenya:Nairobi"
                     /lat_lon="1.3345 S 36.7331 E"
                     /collection_date="2015-06-27"
                     /collected_by="Joerg Jores, Anna Lacasta, Anne Liljander,
                     Paul Ssajjakambwe (International Livestock Research
                     Institute, ILRI)"
     gene            209..397
                     /locus_tag="P3U76_RS14160"
                     /old_locus_tag="P3U76_14160"
     CDS             209..397
                     /locus_tag="P3U76_RS14160"
                     /old_locus_tag="P3U76_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001039248.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     gene            complement(723..1304)
                     /locus_tag="P3U76_RS14165"
                     /old_locus_tag="P3U76_14165"
     CDS             complement(723..1304)
                     /locus_tag="P3U76_RS14165"
                     /old_locus_tag="P3U76_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000991705.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIAD
                     KNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDIEN
                     KKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVAKKL
                     AKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
                     /name="hypothetical protein"
     gene            1756..2751
                     /locus_tag="P3U76_RS14170"
                     /old_locus_tag="P3U76_14170"
     CDS             1756..2751
                     /locus_tag="P3U76_RS14170"
                     /old_locus_tag="P3U76_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001199892.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     Typing          2822..2986
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.42e-81"
     Typing          1757..2751
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.9"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 actggttttg ctgaaatttt tgtttgtgaa attaccatat atttatttgg ctcatatttg
       61 cgttttaaga gcttgtatat atttttaagt taaaaaccta tatgataccc ccctaaacct
      121 ttaaaatacc cccttaaaac tcaaaataaa ggtatttata atttgagtaa gtcttgtgat
      181 aaagtttttt aaaaaaggag tgtttatttt gaataaaaac acagtgttag atgaaggtta
      241 cgttatagca actattttaa atgttttctt tttacttgga ttaattttta tatctcattt
      301 ggaaaattta tatatattaa ttccgtatgt tattttgatg ggaataaatg ctatttattt
      361 ggttgttaag gttatgaatt tcaaaaagaa taattagaat atttgagggg attttgaaac
      421 gagtttcttc ttgttttcac actgtttttt tattcctatt ggtgttgttg cttacttttt
      481 gtttttctat aaagatgatg cttttgacga gatggaagaa aaatatgatt atcatgaaga
      541 taataaaaaa tagacgacgc atttatgccg agaaaattta ttgatgttga gaagaaccct
      601 taactaaact tgcagacgaa tgtcggcata gcgtgagcta ttaagccgac catgcgacaa
      661 gttttgggat tgttaagggt tccgaggctc aacgtcaata aagcaattgg aataaatagt
      721 atctaaagta acccgtattt aagattacat tgtacatcct tcgaaaccat agtagctgcg
      781 attccagcta ggtttccttt tatcccaatt ttagctaatt tttttgctac gccctttaag
      841 tttttggctt taacatcttc aaaaactgct gtgattatgt ttccagttaa aagttctcca
      901 tatgagttta aaacttcaga atagaaacag tcaccgctat ttttgtagtt cccaccaact
      961 gctctaggtg atattgaact agttttcttt ttattctcta tgtcgttttt tagtttttgg
     1021 aatccttcag gaacatatcc atatcgagat ttggcatttt ctagattaac atctttagct
     1081 gttccgtctt tatctaaaat aataattttg ccatatatgt attttagttc tttttcaatt
     1141 tctttttcgt ttaaagtttc aacgttttta tctgcaatgt tatttttgtt ttgggcttga
     1201 gcttctttga aattaaaatc caagaccatt gaaagtatgg ttaaaatact taaagatact
     1261 actaaaatct ttcttagtga tatactgttt tgaattgcat tcattgtaaa tgcccccttg
     1321 ttttgttatt taaatgaatt caatataata ttaacatata aaaacaaagg agtttaatat
     1381 gaataagttt ttttactttt tgattatttt attatctgta tttttaattt ttaattttgt
     1441 taattcgatt ttatttgata atccttttga ctttttagaa agttttattt attcattggt
     1501 tttcacatta atttatttag gtttagaggg tcataaaaag aagaaggaat aggttgtttt
     1561 ttgaaacgag tgtgaacgag tttcttcttg tcttgatact atatagaaat aactcgattt
     1621 tatatatata gctgtaactg ttgatattac agtgtttaaa cgtgtttttg tgcgtgaaag
     1681 gaaaatttga caataaaaaa ccccagttat attattaagg tgtcgaatct taaataatac
     1741 tgggggtctt tttatatgca atataatact actagaagta taaccgaaaa tcaagataat
     1801 aaaacgttaa aagatatgac gaaaagtggg aaacaacgcc catggagaga aaagaaaata
     1861 gataatgtaa gctatgcaga tatactagaa attttaaaaa tcaaaaaggc ttttaatgta
     1921 aaacaatgtg gtaatatttt agaatttaag ccaactgatg aaggctattt gaagttacat
     1981 aagacatggt tttgtaaatc aaaattatgt ccggtttgta attggagacg tgctatgaaa
     2041 aatagttatc aagctcaaaa agtgattgaa aaagtaatta aggaaaagcc aaaagcacgt
     2101 tggttgtttt taacactttc aacaaaaaat gcgatagatg gagatacttt agaacaaagt
     2161 ttgaagcatc taactaaagc atttgatagg ttgagtagat ataaaaaggt taaacaaaat
     2221 cttgttggat ttatgcgttc aacagaagtt accgttaata aaaatgacgg tagttataat
     2281 cagcacatgc atgttttgtt atgtgttgaa aatgcatatt ttagaaaaaa agagaattat
     2341 ataactcaag aagaatgggt taatttatgg caaagagcat tacaagttga ctatcgacct
     2401 gttgctaatg ttaaagcgat caaaccgaat agaaaaggcg ataaagacat tgaatcggca
     2461 atcaaagaga cctcaaaata ttcggttaaa tcatctgatt ttttaactga tgatgatgaa
     2521 aaaaatcaag aaattgtaag tgatttagaa aaaggtttgt atcgaaaacg tatgttaagt
     2581 tatggtggat tgcttaaaca aaaacataaa attttaaact tagacgatgt cgaagatggt
     2641 aatttgatta atgcaagtga tgaagataaa acaacagacg aagaagaaaa agcacattca
     2701 attaccgcaa tttggaattt cgaaaagcaa aattattatt taagacatta gtgttgacta
     2761 atgtcttttt tgttgatttt ttataaaaaa gtactgtctt atttttgtga caaatgctgt
     2821 atgtagtgtc acaaaaataa gacaaacgca atatattgtg tcacaaaaat aagacagtac
     2881 agctttgtat gatatcactt taaaatacct taaaacccct ggaatgtctg gctttgccag
     2941 acctatcatt tttgaatgat agcaaattct ccttatgctc ttacggagtt tttagagaaa
     3001 aattaaaaat tctcgatttt tgataaaaaa cgccctgcag gaatttagaa aaacatgtgg
     3061 aagttttaaa ggattttatg ctaattttta atttgcatgt aaatgggcag tgttaaaaaa
     3121 ttagtcactg gttttgctga aatttttgtt tgtgaaatta ccatatattt attt
//
