LOCUS       NZ_CP120117             3126 bp    DNA     circular CON 06-JAN-2024
DEFINITION  Staphylococcus aureus strain Dog101 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP120117
VERSION     NZ_CP120117.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33696793
            Assembly: GCF_034034275.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Canine Staphylococcaceae circulating in a Kenyan animal shelter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
  REMARK    Annotation added by database at submitter's request
REFERENCE   3  (bases 1 to 3126)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2022
            Assembly Method                   :: Flye v. v2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 302x
            Sequencing Technology             :: PacBio Sequel II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/06/2024 10:52:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,752
            Genes (coding)                    :: 2,665
            CDSs (with protein)               :: 2,665
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 46 of 87
            Pseudo Genes (incomplete)         :: 35 of 87
            Pseudo Genes (internal stop)      :: 21 of 87
            Pseudo Genes (multiple problems)  :: 15 of 87
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP120117.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="Dog101"
                     /isolation_source="nasal swab"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed2"
                     /geo_loc_name="Kenya:Nairobi"
                     /lat_lon="1.3345 S 36.7331 E"
                     /collection_date="2015-06-27"
                     /collected_by="Joerg Jores, Anna Lacasta, Anne Liljander,
                     Paul Ssajjakambwe (International Livestock Research
                     Institute, ILRI)"
     gene            join(2589..3126,1..44)
                     /locus_tag="P3U56_RS14160"
                     /old_locus_tag="P3U56_14160"
     CDS             join(2589..3126,1..44)
                     /locus_tag="P3U56_RS14160"
                     /old_locus_tag="P3U56_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000991705.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIAD
                     KNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDIEN
                     KKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVAKKL
                     AKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
                     /name="hypothetical protein"
     gene            complement(370..558)
                     /locus_tag="P3U56_RS14165"
                     /old_locus_tag="P3U56_14165"
     CDS             complement(370..558)
                     /locus_tag="P3U56_RS14165"
                     /old_locus_tag="P3U56_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001039248.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     gene            complement(1142..2137)
                     /locus_tag="P3U56_RS14170"
                     /old_locus_tag="P3U56_14170"
     CDS             complement(1142..2137)
                     /locus_tag="P3U56_RS14170"
                     /old_locus_tag="P3U56_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001199892.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     Typing          complement(908..1072)
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.42e-81"
     Typing          complement(1143..2137)
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.9"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttcgaagga tgtacaatgt aatcttaaat acgggttact ttagatacta tttattccaa
       61 ttgctttatt gacgttgagc ctcggaaccc ttaacaatcc caaaacttgt cgcatggtcg
      121 gcttaatagc tcacgctatg ccgacattcg tctgcaagtt tagttaaggg ttcttctcaa
      181 catcaataaa ttttctcggc ataaatgcgt cgtctatttt ttattatctt catgataatc
      241 atatttttct tccatctcgt caaaagcatc atctttatag aaaaacaaaa agtaagcaac
      301 aacaccaata ggaataaaaa aacagtgtga aaacaagaag aaactcgttt caaaatcccc
      361 tcaaatattc taattattct ttttgaaatt cataacctta acaaccaaat aaatagcatt
      421 tattcccatc aaaataacat acggaattaa tatatataaa ttttccaaat gagatataaa
      481 aattaatcca agtaaaaaga aaacatttaa aatagttgct ataacgtaac cttcatctaa
      541 cactgtgttt ttattcaaaa taaacactcc ttttttaaaa aactttatca caagacttac
      601 tcaaattata aataccttta ttttgagttt taagggggta ttttaaaggt ttaggggggt
      661 atcatatagg tttttaactt aaaaatatat acaagctctt aaaacgcaaa tatgagccaa
      721 ataaatatat ggtaatttca caaacaaaaa tttcagcaaa accagtgact aattttttaa
      781 cactgcccat ttacatgcaa attaaaaatt agcataaaat cctttaaaac ttccacatgt
      841 ttttctaaat tcctgcaggg cgttttttat caaaaatcga gaatttttaa tttttctcta
      901 aaaactccgt aagagcataa ggagaatttg ctatcattca aaaatgatag gtctggcaaa
      961 gccagacatt ccaggggttt taaggtattt taaagtgata tcatacaaag ctgtactgtc
     1021 ttatttttgt gacacaatat attgcgtttg tcttattttt gtgacactac atacagcatt
     1081 tgtcacaaaa ataagacagt acttttttat aaaaaatcaa caaaaaagac attagtcaac
     1141 actaatgtct taaataataa ttttgctttt cgaaattcca aattgcggta attgaatgtg
     1201 ctttttcttc ttcgtctgtt gttttatctt catcacttgc attaatcaaa ttaccatctt
     1261 cgacatcgtc taagtttaaa attttatgtt tttgtttaag caatccacca taacttaaca
     1321 tacgttttcg atacaaacct ttttctaaat cacttacaat ttcttgattt ttttcatcat
     1381 catcagttaa aaaatcagat gatttaaccg aatattttga ggtctctttg attgccgatt
     1441 caatgtcttt atcgcctttt ctattcggtt tgatcgcttt aacattagca acaggtcgat
     1501 agtcaacttg taatgctctt tgccataaat taacccattc ttcttgagtt atataattct
     1561 ctttttttct aaaatatgca ttttcaacac ataacaaaac atgcatgtgc tgattataac
     1621 taccgtcatt tttattaacg gtaacttctg ttgaacgcat aaatccaaca agattttgtt
     1681 taaccttttt atatctactc aacctatcaa atgctttagt tagatgcttc aaactttgtt
     1741 ctaaagtatc tccatctatc gcattttttg ttgaaagtgt taaaaacaac caacgtgctt
     1801 ttggcttttc cttaattact ttttcaatca ctttttgagc ttgataacta tttttcatag
     1861 cacgtctcca attacaaacc ggacataatt ttgatttaca aaaccatgtc ttatgtaact
     1921 tcaaatagcc ttcatcagtt ggcttaaatt ctaaaatatt accacattgt tttacattaa
     1981 aagccttttt gatttttaaa atttctagta tatctgcata gcttacatta tctattttct
     2041 tttctctcca tgggcgttgt ttcccacttt tcgtcatatc ttttaacgtt ttattatctt
     2101 gattttcggt tatacttcta gtagtattat attgcatata aaaagacccc cagtattatt
     2161 taagattcga caccttaata atataactgg ggttttttat tgtcaaattt tcctttcacg
     2221 cacaaaaaca cgtttaaaca ctgtaatatc aacagttaca gctatatata taaaatcgag
     2281 ttatttctat atagtatcaa gacaagaaga aactcgttca cactcgtttc aaaaaacaac
     2341 ctattccttc ttctttttat gaccctctaa acctaaataa attaatgtga aaaccaatga
     2401 ataaataaaa ctttctaaaa agtcaaaagg attatcaaat aaaatcgaat taacaaaatt
     2461 aaaaattaaa aatacagata ataaaataat caaaaagtaa aaaaacttat tcatattaaa
     2521 ctcctttgtt tttatatgtt aatattatat tgaattcatt taaataacaa aacaaggggg
     2581 catttacaat gaatgcaatt caaaacagta tatcactaag aaagatttta gtagtatctt
     2641 taagtatttt aaccatactt tcaatggtct tggattttaa tttcaaagaa gctcaagccc
     2701 aaaacaaaaa taacattgca gataaaaacg ttgaaacttt aaacgaaaaa gaaattgaaa
     2761 aagaactaaa atacatatat ggcaaaatta ttattttaga taaagacgga acagctaaag
     2821 atgttaatct agaaaatgcc aaatctcgat atggatatgt tcctgaagga ttccaaaaac
     2881 taaaaaacga catagagaat aaaaagaaaa ctagttcaat atcacctaga gcagttggtg
     2941 ggaactacaa aaatagcggt gactgtttct attctgaagt tttaaactca tatggagaac
     3001 ttttaactgg aaacataatc acagcagttt ttgaagatgt taaagccaaa aacttaaagg
     3061 gcgtagcaaa aaaattagct aaaattggga taaaaggaaa cctagctgga atcgcagcta
     3121 ctatgg
//
